LOCUS       NM_018263              12849 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens ASXL transcriptional regulator 2 (ASXL2), transcript
            variant 1, mRNA.
ACCESSION   NM_018263 XM_351425 XM_352944
VERSION     NM_018263.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12849)
  AUTHORS   Abuasab,T., Borthakur,G., Kanagal-Shamanna,R., Masarova,L.,
            Patel,K., Takahashi,K., Bose,P., Villarreal,J., Pierce,S.,
            Kadia,T., Garcia-Manero,G., Short,N.J., DiNardo,C., Daver,N.,
            Ravandi,F., Kantarjian,H., Verstovsek,S. and Yilmaz,M.
  TITLE     Exploring the landscape of somatic ASXL2 mutations in myeloid
            neoplasms: Frequency and clinical implications
  JOURNAL   Am J Hematol 99 (7), 1434-1436 (2024)
   PUBMED   38613831
  REMARK    GeneRIF: Exploring the landscape of somatic ASXL2 mutations in
            myeloid neoplasms: Frequency and clinical implications.
REFERENCE   2  (bases 1 to 12849)
  AUTHORS   Peng,H., Cassel,J., McCracken,D.S., Prokop,J.W., Sementino,E.,
            Cheung,M., Collop,P.R., Polo,A., Joshi,S., Mandell,J.P.,
            Ayyanathan,K., Hinds,D., Malkowicz,S.B., Harbour,J.W.,
            Bowcock,A.M., Salvino,J., Kennedy,E.J., Testa,J.R. and
            Rauscher,F.J. 3rd.
  TITLE     Kinetic Characterization of ASXL1/2-Mediated Allosteric Regulation
            of the BAP1 Deubiquitinase
  JOURNAL   Mol Cancer Res 19 (7), 1099-1112 (2021)
   PUBMED   33731362
  REMARK    GeneRIF: Kinetic Characterization of ASXL1/2-Mediated Allosteric
            Regulation of the BAP1 Deubiquitinase.
REFERENCE   3  (bases 1 to 12849)
  AUTHORS   Cuddapah,V.A., Dubbs,H.A., Adang,L., Kugler,S.L., McCormick,E.M.,
            Zolkipli-Cunningham,Z., Ortiz-Gonzalez,X.R., McCormack,S.,
            Zackai,E., Licht,D.J., Falk,M.J. and Marsh,E.D.
  TITLE     Understanding the phenotypic spectrum of ASXL-related disease: Ten
            cases and a review of the literature
  JOURNAL   Am J Med Genet A 185 (6), 1700-1711 (2021)
   PUBMED   33751773
  REMARK    GeneRIF: Understanding the phenotypic spectrum of ASXL-related
            disease: Ten cases and a review of the literature.
REFERENCE   4  (bases 1 to 12849)
  AUTHORS   Cao,H.Q. and Tuo,J.B.
  TITLE     [The Relationship between ASXL2 and ZBTB7A Gene Mutations and
            Prognosis in Patients with Acute Myeloid Leukemia]
  JOURNAL   Zhongguo Shi Yan Xue Ye Xue Za Zhi 29 (2), 450-455 (2021)
   PUBMED   33812414
  REMARK    GeneRIF: [The Relationship between ASXL2 and ZBTB7A Gene Mutations
            and Prognosis in Patients with Acute Myeloid Leukemia].
REFERENCE   5  (bases 1 to 12849)
  AUTHORS   Katoh,M.
  TITLE     Functional proteomics of the epigenetic regulators ASXL1, ASXL2 and
            ASXL3: a convergence of proteomics and epigenetics for
            translational medicine
  JOURNAL   Expert Rev Proteomics 12 (3), 317-328 (2015)
   PUBMED   25835095
  REMARK    GeneRIF: ASXL1, ASXL2 and ASXL3 are epigenetic scaffold proteins
            that are involved in the pathogenesis of non-cancerous diseases and
            cancers.[Review]
            Review article
REFERENCE   6  (bases 1 to 12849)
  AUTHORS   Park,U.H., Seong,M.R., Kim,E.J., Hur,W., Kim,S.W., Yoon,S.K. and
            Um,S.J.
  TITLE     Reciprocal regulation of LXRalpha activity by ASXL1 and ASXL2 in
            lipogenesis
  JOURNAL   Biochem Biophys Res Commun 443 (2), 489-494 (2014)
   PUBMED   24321552
REFERENCE   7  (bases 1 to 12849)
  AUTHORS   Lai,H.L. and Wang,Q.T.
  TITLE     Additional sex combs-like 2 is required for polycomb repressive
            complex 2 binding at select targets
  JOURNAL   PLoS One 8 (9), e73983 (2013)
   PUBMED   24040135
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 12849)
  AUTHORS   Park,U.H., Yoon,S.K., Park,T., Kim,E.J. and Um,S.J.
  TITLE     Additional sex comb-like (ASXL) proteins 1 and 2 play opposite
            roles in adipogenesis via reciprocal regulation of peroxisome
            proliferator-activated receptor {gamma}
  JOURNAL   J Biol Chem 286 (2), 1354-1363 (2011)
   PUBMED   21047783
  REMARK    GeneRIF: ASXL1 represses, whereas ASXL2 increases, the expression
            of adipogenic genes, most of which are PPARgamma targets
REFERENCE   9  (bases 1 to 12849)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     Identification and characterization of ASXL2 gene in silico
  JOURNAL   Int J Oncol 23 (3), 845-850 (2003)
   PUBMED   12888926
REFERENCE   10 (bases 1 to 12849)
  AUTHORS   Porter,J.M., Pena,L.D.M., Spillmann,R.C., Johnson,A. and Shashi,V.
  TITLE     Shashi-Pena Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   39527683
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX647527.1, AB051472.2 and
            AC010150.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Apr 4, 2019 this sequence version replaced NM_018263.5.
            
            Summary: This gene encodes a member of a family of epigenetic
            regulators that bind various histone-modifying enzymes and are
            involved in the assembly of transcription factors at specific
            genomic loci. Naturally occurring mutations in this gene are
            associated with cancer in several tissue types (breast, bladder,
            pancreas, ovary, prostate, and blood). This gene plays an important
            role in neurodevelopment, cardiac function, adipogenesis, and
            osteoclastogenesis. [provided by RefSeq, Feb 2017].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CR933613.1, BX648218.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000435504.9/ ENSP00000391447.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                BX647527.1         30-73
            45-7218             AB051472.2         1-7174
            7219-12849          AC010150.3         55104-60734
FEATURES             Location/Qualifiers
     source          1..12849
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p23.3"
     gene            1..12849
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /note="ASXL transcriptional regulator 2"
                     /db_xref="GeneID:55252"
                     /db_xref="HGNC:HGNC:23805"
                     /db_xref="MIM:612991"
     exon            1..322
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /inference="alignment:Splign:2.1.0"
     CDS             266..4573
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /note="isoform 1 is encoded by transcript variant 1;
                     polycomb group protein ASXH2; putative Polycomb group
                     protein ASXL2; additional sex combs-like protein 2;
                     additional sex combs like 2, transcriptional regulator"
                     /codon_start=1
                     /product="putative Polycomb group protein ASXL2 isoform 1"
                     /protein_id="NP_060733.4"
                     /db_xref="CCDS:CCDS92720.1"
                     /db_xref="GeneID:55252"
                     /db_xref="HGNC:HGNC:23805"
                     /db_xref="MIM:612991"
                     /translation="MREKGRRKKGRTWAEAAKTVLEKYPNTPMSHKEILQVIQREGLK
                     EIRSGTSPLACLNAMLHTNSRGEEGIFYKVPGRMGVYTLKKDVPDGVKELSEGSEESS
                     DGQSDSQSSENSSSSSDGGSNKEGKKSRWKRKVSSSSPQSGCPSPTIPAGKVISPSQK
                     HSKKALKQALKQQQQKKQQQQCRPSISISSNQHLSLKTVKAASDSVPAKPATWEGKQS
                     DGQTGSPQNSNSSFSSSVKVENTLLGLGKKSFQRSERLHTRQMKRTKCADIDVETPDS
                     ILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTS
                     AAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVEPWKEQFFESYYGQSSGLSLEDSKKL
                     TASPSDPKVKKTPAEQPKSMPVSEASLIRIVPVVSQSECKEEALQMSSPGRKEECESQ
                     GEVQPNFSTSSEPLLSSALNTHELSSILPIKCPKDEDLLEQKPVTSAEQESEKNHLTT
                     ASNYNKSESQESLVTSPSKPKSPGVEKPIVKPTAGAGPQETNMKEPLATLVDQSPESL
                     KRKSSLTQEEAPVSWEKRPRVTENRQHQQPFQVSPQPFLNRGDRIQVRKVPPLKIPVS
                     RISPMPFHPSQVSPRARFPVSITSPNRTGARTLADIKAKAQLVKAQRAAAAAAAAAAA
                     AASVGGTIPGPGPGGGQGPGEGGEGQTARGGSPGSDRVSETGKGPTLELAGTGSRGGT
                     RELLPCGPETQPQSETKTTPSQAQPHSVSGAQLQQTPPVPPTPAVSGACTSVPSPAHI
                     EKLDNEKLNPTRATATVASVSHPQGPSSCRQEKAPSPTGPALISGASPVHCAADGTVE
                     LKAGPSKNIPNPSASSKTDASVPVAVTPSPLTSLLTTATLEKLPVPQVSATTAPAGSA
                     PPSSTLPAASSLKTPGTSLNMNGPTLRPTSSIPANNPLVTQLLQGKDVPMEQILPKPL
                     TKVEMKTVPLTAKEERGMGALIATNTTENSTREEVNERQSHPATQQQLGKTLQSKQLP
                     QVPRPLQLFSAKELRDSSIDTHQYHEGLSKATQDQILQTLIQRVRRQNLLSVVPPSQF
                     NFAHSGFQLEDISTSQRFMLGFAGRRTSKPAMAGHYLLNISTYGRGSESFRRTHSVNP
                     EDRFCLSSPTEALKMGYTDCKNATGESSSSKEDDTDEESTGDEQESVTVKEEPQVSQS
                     AGKGDTSSGPHSRETLSTSDCLASKNVKAEIPLNEQTTLSKENYLFTRGQTFDEKTLA
                     RDLIQAAQKQMAHAVRGKAIRSSPELFSSTVLPLPADSPTHQPLLLPPLQTPKLYGSP
                     TQIGPSYRGMINVSTSSDMDHNSAVPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSH
                     GQTIPVQAFSEENSIEGTPSKCYCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR"
     exon            323..405
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /inference="alignment:Splign:2.1.0"
     exon            406..408
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /inference="alignment:Splign:2.1.0"
     exon            409..517
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /inference="alignment:Splign:2.1.0"
     exon            518..668
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /inference="alignment:Splign:2.1.0"
     exon            669..769
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /inference="alignment:Splign:2.1.0"
     exon            770..896
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /inference="alignment:Splign:2.1.0"
     exon            897..1040
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1204
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1301
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1407
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..2125
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /inference="alignment:Splign:2.1.0"
     exon            2126..12849
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9105..9110
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /note="hexamer: ATTAAA"
     polyA_site      9115
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /note="major polyA site"
     regulatory      12825..12830
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /note="hexamer: AATAAA"
     polyA_site      12849
                     /gene="ASXL2"
                     /gene_synonym="ASXH2; SHAPNS"
                     /note="major polyA site"
ORIGIN      
        1 acatgaccgt agcggcagcc tgtacagtaa gaacccaata tggcagcggc ggtagcagtg
       61 gcaacggcag caagaggacc ggccgcccca tagacccccc cgcgcaccac ccccgccgct
      121 tcctctgctt gggtgccccc ccggtctgac ttcccttttc tcccaccttt cccggcaccg
      181 cgggaaaagc agcgcagggc agagcaggca gggagggcgg ccggagcccg gacacgggag
      241 cctcccagtc agttcaagac ccgacatgag ggaaaaggga cgtaggaaga agggcaggac
      301 ctgggcggag gccgccaaga cggtcttaga aaaatacccc aatacaccca tgagtcataa
      361 agaaattctt caagttatcc agagagaagg actaaaagaa atcagaagtg ggacttctcc
      421 tcttgcatgc ctgaatgcaa tgcttcacac aaactccaga ggtgaagagg gcatcttcta
      481 taaggttcca ggtagaatgg gagtatatac tttgaagaaa gatgtgccgg atggggtgaa
      541 agagctgtca gaaggttcag aagaaagcag tgatggtcag tcagattccc agagttctga
      601 gaacagcagc agcagcagtg atggtggcag caacaaggag ggaaaaaaga gcaggtggaa
      661 aaggaaagta tcgtcgtcct ccccgcagtc aggctgccca tcacccacca ttccagcagg
      721 taaagtcatt tctccatcac agaagcacag caagaaggca ctaaagcagg cgctaaagca
      781 gcaacagcag aagaagcagc agcagcaatg caggccaagc atatccatct cctccaacca
      841 gcatctctca ctaaagactg tcaaagcagc cagtgactct gtacctgcca aacctgcaac
      901 atgggaagga aagcaatctg atggacagac aggcagccct caaaactcaa actccagctt
      961 ttcttcctca gttaaagtgg aaaatacttt actaggcttg gggaagaagt cattccagag
     1021 atctgagaga ctccatacca gacaaatgaa aagaactaaa tgtgctgaca ttgacgttga
     1081 gacaccggac tccattctgg ttaatacaaa tctgcgagca ctgatcaaca agcacacatt
     1141 ttcagtcctt cctggagatt gccagcaacg actgctttta ctactcccag aggtagatcg
     1201 acaggttggt ccagatggtt taatgaagtt aaatggctca gcccttaaca atgaattctt
     1261 cacttcagca gcccaaggct ggaaggaaag actctcagaa ggtgagttta cacctgagat
     1321 gcaggtgaga attcgacaag agattgagaa ggagaaaaaa gtggagccat ggaaagaaca
     1381 attctttgaa agctactatg ggcagagttc tggcctgagc cttgaagatt ctaagaaatt
     1441 gacagcttct cccagtgatc ccaaagtaaa gaaaacccca gctgaacaac caaaatccat
     1501 gcctgtgtca gaggcctctc ttatcagaat agttccagta gtctcccagt cagagtgtaa
     1561 agaagaagca ttgcaaatgt catcaccagg cagaaaagaa gagtgtgaaa gccaaggtga
     1621 agtgcagccg aacttctcca catcttcaga gcccctgctt tcctcagctc tcaatacaca
     1681 tgagcttagc agcattcttc ccatcaagtg cccaaaggat gaggatctct tggagcagaa
     1741 gccagtcacc tctgctgaac aggaatctga gaagaaccat ctcaccacag cttctaatta
     1801 taacaaaagt gaaagccaag aatctttagt tacatcgcca agcaaaccca agagtcctgg
     1861 ggttgaaaaa ccaatagtga agcccacagc aggagcgggt ccacaggaga ctaatatgaa
     1921 agaacctcta gcaactcttg ttgatcagag cccagaaagc ctcaagagga agtcttccct
     1981 cacccaagaa gaggcccctg tgagctggga gaagaggcca cgtgtcactg agaatcgcca
     2041 gcaccagcag ccatttcagg tctcaccaca gccctttctc aatagagggg acagaatcca
     2101 ggtgcgaaaa gtaccacctc tcaagatccc ggtctccaga atctccccca tgccgtttca
     2161 tccatcgcag gtctctccca gggctcgttt tccagtctcc atcactagtc ctaacagaac
     2221 aggagccaga actcttgcag acatcaaagc aaaagcccaa ctggtcaaag cacagagggc
     2281 agcagctgcc gctgccgccg cagctgctgc agccgcctca gttggaggga ccattccagg
     2341 acctggccca gggggtggac aaggtccagg agagggtggt gaagggcaga ctgctagagg
     2401 aggcagtcca ggctcagaca gagtcagtga aactggaaag ggccccacac tggaactggc
     2461 aggaactgga agcaggggag gtacgagaga gcttttaccc tgtggtccag agactcagcc
     2521 ccagtctgag accaagacca ccccaagcca ggcacagcct catagtgtct ctggagcaca
     2581 actacagcaa acccccccag tgcctccaac acctgccgtc agtggagcat gcacaagtgt
     2641 cccatcacca gcccacatag agaaattgga taatgaaaaa ctgaacccca ccagagcaac
     2701 agccacagtg gcctctgtca gccatccaca agggcccagt agttgcagac aggagaaagc
     2761 accttctcca acaggtcctg ctctaatctc aggtgcctca cctgttcatt gtgcagctga
     2821 tggcacagtt gagctcaaag caggtcctag taagaatata cctaaccctt cagcctcatc
     2881 aaagacagat gctagtgtgc cagtggctgt aactccctcc cctttaacat ctttattgac
     2941 cacagccact ttagaaaagc ttcctgtacc ccaggtcagt gcaactacag cacctgctgg
     3001 atcagctcca ccctcgagca ctttgccagc agcttctagc cttaaaaccc caggaacttc
     3061 tttaaacatg aatggaccca ctttaagacc aacctctagt atccctgcta ataatccttt
     3121 agtgactcag ctgcttcaag gcaaagatgt tcccatggag caaattctgc ctaaacctct
     3181 caccaaagtt gaaatgaaaa cggttccact gactgcaaaa gaggaaaggg ggatgggagc
     3241 gctcatagct accaacacaa cagaaaatag caccagagag gaagttaatg agagacagtc
     3301 ccatccagct acgcagcagc agctgggcaa aaccttgcaa agtaagcagc tcccccaggt
     3361 tccaaggccc cttcagctct tttcagctaa ggagctgagg gactccagca ttgacacaca
     3421 ccaataccac gaaggactaa gtaaagcaac ccaagatcag atccttcaga ctctcattca
     3481 gagggttcgg aggcagaatc ttctctcagt tgtgccgccc tcacagttca acttcgctca
     3541 ctcaggtttc cagctggaag acatctccac aagccagagg ttcatgctgg gttttgctgg
     3601 cagaaggaca tccaaacctg caatggcagg gcactactta ctgaatattt ctacctacgg
     3661 ccggggctca gagagcttta ggaggaccca ttctgtaaac cctgaagatc gtttttgtct
     3721 aagcagcccc actgaagcct tgaaaatggg atatacagac tgtaaaaatg caacaggaga
     3781 gagtagcagc agcaaagaag atgacactga tgaggaaagt actggtgatg agcaggaatc
     3841 tgtcacagtg aaagaggagc cccaggtttc ccagagtgct ggcaagggtg acacaagttc
     3901 aggacctcac agcagggaaa ctctatctac cagtgattgc ttagctagca agaatgtgaa
     3961 ggctgagata ccattgaatg agcaaaccac tttaagtaag gagaattacc tgttcactag
     4021 aggccaaaca tttgatgaaa agaccctagc cagagattta attcaggcag cacagaagca
     4081 gatggctcat gcagtgagag gtaaggcaat ccgtagcagc cccgagcttt tcagttctac
     4141 tgttcttcct ctgcctgcag acagccccac ccaccagccc ctactccttc cacccctgca
     4201 aaccccgaag ttgtatggaa gccccaccca gatagggcca agctatagag gcatgatcaa
     4261 tgtctccacc tcatctgaca tggaccataa ctctgctgta ccaggtagcc aggtatctag
     4321 caatgtaggt gatgtcatgt cattttcagt gactgtcact accatccctg ctagccaagc
     4381 tatgaatccc agcagccatg gccagaccat tcctgttcag gcgttctccg aagagaacag
     4441 catagagggc acgccttcga aatgttactg ccgcttgaaa gccatgatca tgtgcaaagg
     4501 ctgtggcgct ttctgccatg atgattgcat cggcccctcc aaactgtgcg tctcctgcct
     4561 tgtcgttcgg taatgagact agaaagagat acactgtaaa ggagggggaa gggaagggtt
     4621 gaccagttgg gttttttgcg tccttttgga atcacagaaa taaacaagta ggtttcagtt
     4681 gtcttcagag acaaatgtta cttaatcagg aatacaaagt acagattttt tacattttag
     4741 aggaagagca ccttgtatag taagtctaag tcaagcaaga ctttgtgaat ttgtgacaag
     4801 tttgcactta caaaatcatg taaatatgtc acattttgtt taacattatt tttccaagtg
     4861 tttaggtaat aatgtattac tttgaattca gattaatgtt ctactacttg tgactgagaa
     4921 aagtttaatg ccggcatggt aaaaggaagc tgtctccctt ctgacatttc ctaggaagct
     4981 aggaaaagga aagtgaagga atggtctaaa gaaatgacca ttcacactga ttttgtctgg
     5041 acagtctggc cgcaggttat tcatagatta ttcagccttt gcaggacttg gattcagggt
     5101 tttactcagt ccctttacct taggtggaat cttcttaaat tgaaattttt ggtaaggaat
     5161 tcatttcaca ggtagtgttt cagactctga aagccctgac ttggttcttg gcttctactg
     5221 tactagttac tagttactgg tactgttgcc aagcaatctg cttggaattt gtggatccct
     5281 gctgttccct aatcccaccc cctgccctga gacagtgaat gtagtccgtg aagggagtgc
     5341 ctctctggga ccccctgtgt tgttacaggc tgtgcagtgc aacaattcca gcaaaaatac
     5401 cctatccccg cacttagtca ttcgtggtaa ctaacaattt tgaaatactc atataaaatg
     5461 aacaggaaag tgttagtgat tgggtgattt gagctacatc ttgttgagca aggggagtga
     5521 aaccacagaa actgttaaat tgaacgtaaa taactttgga aaacagtttt gacactagaa
     5581 caaggtcgct tctcttttcc tcttctctcc caacctgtta acctagtgct agatagtcag
     5641 ctgctgttgc aggtagaaaa gctcaccaat gccacatttg ttcattctgt tcaacctgtt
     5701 ttgacctccc aggcatctga acaggatgac atcatcttat tatatcttac tttacacata
     5761 cgtgtatgta cacacacaca cacaatacaa gacatttttc ctgttttaga aaatatagcc
     5821 cttgtttgga ttactgctgt ctgagcacta gaaatttcta atggaaagag gcctctgaat
     5881 ggctaaggga acatctggaa aggaaggaaa atgagcccta aaggtttttt ggttgcttgt
     5941 ttttgtttct ttttctttct tgtttccttt tttgttttta aatccttctc tagtgactga
     6001 agaaattgtg agtacataat atttaggtca tggttaagga acattaaaat aaacctgcag
     6061 tttaacagaa ttttgtatta aatgggggag tgtcaatttg catcatttgc atattgattt
     6121 caagtctcct aatttggaat gggtaaagta caaatgttaa tgcaacatag tacagttgta
     6181 tttgtccttc atattcagga tgtgatattt acaaaatgca gaacagttta aacttgttaa
     6241 gtcacattcc agattaggag aaaatgggca ataatcatct ctgttttgcc cccattctac
     6301 tacccaccac tgcccacccc acccccacat cacacactct tcgatgcttt cccacagccc
     6361 ctgctccgct cctctccgta agaaactcac ttcactgttt ttcacttttc acttctggct
     6421 tttttgtggc ccaaggccaa tataggatta atatctgtgt tccagcttca gccagaacga
     6481 tatgtctgtc catctttctc ctttcttcca gcacacacag ctaagagata agaaggctgc
     6541 aaggattggg gataaggagt atattttcaa gccaagaacc agtagaaaac agaaataatg
     6601 gtgctcccaa atattcagta ccttgggcaa tccaggaagg gtttaaagag acctgttgct
     6661 gcatagaact ctccctgccc taccccttaa ctccacctta gtgccagggc cgagggctgg
     6721 tctgcatgct ggaagaggtt gagagttttg gaattgattc catgctaaat gtcagttacc
     6781 agtttggatt gatgaaagtg atttttctgc tttacctttt tcctgtcaga aaggttttcc
     6841 tctgtgctac ccttaactta gggatgaaca gactataact aggaaaggag gaaaggtatc
     6901 agggattttt aagtccgttt agagagcaaa ccatattgtg cctcttcatc ttgacccagg
     6961 ctcttataga taggtcctaa acattgctgt tcactagatt atcttaacaa ataatactca
     7021 gctaaatatt tgcatttagg aatactccta aaacagatat tgccaactag atacactgcc
     7081 tcaatcttga ccagacaaga gaacagttaa gttctctcag ttgggtagta acttgaatac
     7141 cagtctattt atcagttggt ttcaacacag aggtttcaag tggaaagcca ttcatcttaa
     7201 aagctgaaca gaaaaattaa aaaaaaaaaa aacacattac aatctggcct tttagaaaat
     7261 gaagcagtag aaacaaaacc tttattagta tggtattcca gcagcacctt taaagcaatc
     7321 tttaccttag ttttttgagt tgttttacct ttcatcccaa tctgaaaagt tttaataaat
     7381 gctgagctta ttcttagggt atgacctggc attatttcca gtgactagtc aggcattatg
     7441 cctgatgcat acgcctcccg tgcaccccta tctagccttc agatgtcctc cactgcaggt
     7501 caccccagga gatagcatca ataatatagt ctcagtcttg tttataaaga ttctaagttg
     7561 tctagtaaat aagtctccct gaatcttaaa taattttgga gaattagaat tctccatcct
     7621 attaaacaga aagtaaagcc aaatagcctg gagttgtttg tcctacttgt tccataaccc
     7681 ctatgctcaa gatactgaag ctatctgtgg tatttcatgt aacatttcta agaataagag
     7741 ctggcaaatg ggaaacttgg ggtggagaga gcatacttat tgggctgtga aggcatacgg
     7801 atgtacatgt tttagagctt atctcagatt taaatagcca aaggaagacc tcattgcctt
     7861 ccaggtgaag gtgcaaagtc atacatatta atgacctgac agttaacatt ctgttgttta
     7921 ttattgcctt tctccacctt agagagccat aaataactct ctggtgaagt gatttgctat
     7981 aatgggggtg aggagtgggg agacttctct ggtcattaca ctgctattta tttctagaac
     8041 tcactatcag tgagcagtgc cagagtgttt tctctttgac ctagaggttt cacatcatag
     8101 caaggaggga aaagtctccc catctcctta caactgtgtg ctaagtaagt aacttggcca
     8161 agggtagttt tcagctcaat ttatagaata gtccaaagag actttgagaa acatggtgat
     8221 aggcaggggc ctttcctacc atgtttcaat acctacaaac ctattgaaag agaacagtag
     8281 gtaaaacatt tcctaaagtt gaagcaatag cttcgtagat tcttggttac tttatttttt
     8341 ttaatgcttt acatttgata caactattga agatcaattt taaaaatagc tttccagtaa
     8401 tatattttaa gtaatatata ttttaatatc tgtaaaaagt gacagtggaa gactttgaat
     8461 ttctcatata tggtatgttt gatgtaggac ctcactgtta aggcacaaat tatttcaaag
     8521 gaagttactc taaagacact cagattattt cagaaaaatg caataagggg aatagttttg
     8581 gggtttattt tttattttaa aagaaaattg acttatttac cacaagctat ttttttccct
     8641 tctggctgta aggtttgtac agactggttt ggtaaatgct aaacttttgt gtcttttgcc
     8701 tttttaaagg aattgttaac attggaattg agggtatgta cagagaagtt ggtgtcaaca
     8761 ccatttaaaa agtcatattt tttcacaaat atagaaaatc attttacata agaattttaa
     8821 gtatttgcaa taaatatatg tatatagatt gtatgtattc ctatattctt atttttcatt
     8881 ttattattaa gtaaaagatc attaaaagtg aaaataaaaa ccttggagtt ttttggtgaa
     8941 tcttgaggtt taacatacat ctgagagtgg cgtgggtaag agtcctcagt tactgcctta
     9001 tactcctatg ggatggttcc cacagattgt atggaagaga gtaaaatgag gaacttggtg
     9061 ataaatcagg gtagtgtatt tcttttggaa tttaagtaaa actgattaaa ttttccttat
     9121 ctgtctgtct ccatgttttc tcccttaatc tgttttgtct cctaaccccc taacattact
     9181 ggaaaagtga agaaatgcgg tgcagcctct ttattaatgt gcccattgtt tgctcctaaa
     9241 tcaaagaatg gtaaggaaat atctcggggg aaaaaatagg ttttcctttc tccacagtgg
     9301 ttgacattta ctgccaccac tttccaggcc ttaggggagt gtgaagaaat agtttcttga
     9361 agatgataga aatttttttt tttccgtttc ccagttctct cagaagtgag tttcatagcc
     9421 agtcatgtcc ttcctgcccg tctaatctgg agaggtcaaa gagcaaggaa aataatgtga
     9481 agagaaacac ctgtgccttg attaggttct atttcagcat ttgacacttg aattttgaac
     9541 tcctctgggt caggaagggt agctatggaa atgaggtctg agccagttgg taggagaggt
     9601 tgtctatgtt cccataccga gattgctgtc taatccacga gaactcattt tgtggctgca
     9661 aagtaaatac ttgattttag gatatgatag gagcaaaatg gtgcctacat cgtctttata
     9721 aagaaaagta tatagcccat tttttaacaa atgaataata tatcgaatca ttgatgacct
     9781 aaaagaaaaa ttgttaaggt cagattttcg agagtgtatc ttgatgaccc ataaaactga
     9841 ctttttattg tagttcacta gttcccataa ttgcccagct gtggttactt tcactgaaaa
     9901 gaaaaaatat attttggaag ttaatgtaag gatacattgg cttttttttt ttttctttac
     9961 attggctttt taaaagacag tttttatttt tcagatactg gaatgtcaag tatctgggtt
    10021 gtcccaagac gttggagata attcagcctt ccaagagaac agcagtctag atcttagctg
    10081 aaaatgtgct gtttctaaag gaagcttctt aagtctcagg ggacattagc atcttcataa
    10141 tttccatata taataagaat gaaggtaaaa tatttaaatg tgtataaact aatccataat
    10201 gaccatttac aaagattgac tccccatttc tggtacatat ttatcattat ttagatgggt
    10261 ctcctatctt gtgggaagat aaatttatat tcaggtgatg acatgggtgc ctgcaagtag
    10321 agttggtctg cagctgaggg agaaggcaac ataccagaag acaaggcagc ttcaacctgg
    10381 atccccagct accctttatt tacttcactg agtcagaaac ccgtgttcat tgcttctgag
    10441 actacttgac ctcagctggt ctttagaagt ctggtaactt tttatgtaca aatcatgtta
    10501 tttactaatt caagtattta tagagcacct actgtgcact aagcactaaa gtgctggtta
    10561 tgcaaaggta agcaaaaata gtcatgtaag ttgcagaaag gttttcctaa agtagtgact
    10621 atacagcatg tttttagttc taggctcaag gagtaatact acctttttcc caagataggt
    10681 tgaaaattta aatgagttat gaaatggaag tgtttgagac actggacaca gtagtggcaa
    10741 atacatgaag aaagaaggtc cctagatgaa aaaagtgtgg aaatgaggag ccatattaat
    10801 aaaatgcacc ttttttgaga actattgttg gaatagctaa agttgtctat agaaatttca
    10861 agaaatctct taacatctaa attagaagca ggccaacagc aatctctgtc agtagttaat
    10921 ttaggtcagt aagttagtgt tttgttaaac taaccctttc aaacacagtt gggtaaataa
    10981 tgcagctttc ctgtcactgt tcaaacctaa aagaagctaa agggaatttt tcttaaaaca
    11041 tgattttcat cttatagatg ataagtaact gtatctcttc atttggaatt gttgcatccc
    11101 agtttttctc cataatggat aaaattactt ttgtgtcctt ttctattcta atgtagaagt
    11161 ggcttttcct tttttctctt gagtcacaac ttgaattaaa aagggcttca gcaggactct
    11221 agcgcttttt aaagaagaat ttggtgtatt ccactatgta acgtagtctc tgtccaggaa
    11281 ccaggaggat gagtgtcctc agttttccta atacttggta acatttaata atagattgag
    11341 ttttgctttt gtttttcata gagactgtat agactctgta cttccatgta actttctgcc
    11401 ttaacctaaa aaggcattcc ctggaaggag atcttgtaac aggaagaaaa agcgaaacca
    11461 ggaaaagagc cagaaaggat caacttggag atgggctgtg gctaaatagt gggttttcct
    11521 gaaaagtaac tgcaaccttg cttttcgtgc ttcttcaaac ccaaaagaac ctaaagggaa
    11581 tttttaaaaa tcctgatttt cttcttaatt gtaaaaccta gaggatgcct tagggagaag
    11641 aaagataacc tggaatttgg ttttaaatgg atttaagtaa actttgaatt aagtagctta
    11701 ttccattttt gagagatttc tttttaagat tttagattca cttaatgtaa tgttgaattg
    11761 ccatgaatgt aacctggagt ttgatttgag acattctgtg ggataaggaa tagttttcta
    11821 ttgaactgtg gtggcttttg ccactaatca ggatttaggg ctgtgcacct ccatctgtta
    11881 agtgaaatgg aacttggctt cctaaacact atattagacc aacttgagtc aatactttta
    11941 ttaagtttaa tcagggattc tcaaaatatt taggattcaa attagcccaa ttagaaagca
    12001 gtgcattaaa tgttaccaaa atatctgaag atactttaga actattttac acaaagctta
    12061 tgtctttcct atggaaagta tcatacataa aattttacat tcttttaaaa aacagaatct
    12121 aatgtcttca gatacctaga gcttggaaat tataatagag ttgggctttg acccgaagtc
    12181 agcagttctt ttcattttag attttcaggc aagtttcagt agtttggaga cttcttcagt
    12241 agtgacccca gcttaaccta catataatca ctttgcacac attttaagtt aatcagatgc
    12301 ttccagagtc ttggatttgg ttaccccttt gttagataag gagacagatt ctaggttggg
    12361 acaaacatct taatttattc tctaataata aagtactgat taaagagcaa gttctcagag
    12421 aattcacaaa atttctagaa tccatgaaag atctagagaa cataacaaat gaaactctag
    12481 aaatttgaaa gaaaaaaaaa cacttagctg taagtgcttt gacctatttt ttttaaaaaa
    12541 aaaaaacctg tcttaattct gtgactgcat tgcacccttc accagcacta agagaagtcg
    12601 gaatggaaga aattaccgta tgtaattctt attcacttaa aatctttgtg cgcataggcc
    12661 ttagcacttt gacaaataaa ctctgccttt tagctcctca ctattgtctt ctttcctgtc
    12721 acttttaggc tgtttcattg gtcgtataaa aacaaccttc ttcgaagttg aatgtatgtt
    12781 tctgcacata gacaaaccat attttactga ctcattctat gttgaataaa ggcatacagt
    12841 tttacccta
//
