LOCUS       NM_001083926            2270 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens asparaginase and isoaspartyl peptidase 1 (ASRGL1),
            transcript variant 1, mRNA.
ACCESSION   NM_001083926
VERSION     NM_001083926.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Garcia-Montojo,M., Fathi,S., Rastegar,C., Simula,E.R.,
            Doucet-O'Hare,T., Cheng,Y.H.H., Abrams,R.P.M., Pasternack,N.,
            Malik,N., Bachani,M., Disanza,B., Maric,D., Lee,M.H., Wang,H.,
            Santamaria,U., Li,W., Sampson,K., Lorenzo,J.R., Sanchez,I.E.,
            Mezghrani,A., Li,Y., Sechi,L.A., Pineda,S., Heiman,M., Kellis,M.,
            Steiner,J. and Nath,A.
  TITLE     TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and
            associated with HML-2 expression
  JOURNAL   Nat Commun 15 (1), 4163 (2024)
   PUBMED   38755145
  REMARK    GeneRIF: TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1
            and associated with HML-2 expression.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Wang,X., Wang,Y., Yang,L., Yuan,J., Shen,W., Zhang,W., Wang,J. and
            Tao,K.
  TITLE     ASRGL1 downregulation suppresses hepatocellular carcinoma
            tumorigenesis in a CDK1-dependent manner
  JOURNAL   Dig Liver Dis 55 (7), 955-966 (2023)
   PUBMED   36572570
  REMARK    GeneRIF: ASRGL1 downregulation suppresses hepatocellular carcinoma
            tumorigenesis in a CDK1-dependent manner.
REFERENCE   3  (bases 1 to 2270)
  AUTHORS   Seike,M., Asahara,S.I., Inoue,H., Kudo,M., Kanno,A., Yokoi,A.,
            Suzuki,H., Kimura-Koyanagi,M., Kido,Y. and Ogawa,W.
  TITLE     l-Asparaginase regulates mTORC1 activity via a TSC2-dependent
            pathway in pancreatic beta cells
  JOURNAL   Biochem Biophys Res Commun 652, 121-130 (2023)
   PUBMED   36842323
  REMARK    GeneRIF: l-Asparaginase regulates mTORC1 activity via a
            TSC2-dependent pathway in pancreatic beta cells.
REFERENCE   4  (bases 1 to 2270)
  AUTHORS   Morais,S.B., Pirolla,R.A.S., Frota,N.F., Lourenzoni,M.R.,
            Gozzo,F.C. and Souza,T.A.C.B.
  TITLE     The role of the quaternary structure in the activation of human
            L-asparaginase
  JOURNAL   J Proteomics 224, 103818 (2020)
   PUBMED   32434038
  REMARK    GeneRIF: The role of the quaternary structure in the activation of
            human L-asparaginase.
REFERENCE   5  (bases 1 to 2270)
  AUTHORS   Lv,X.F., Hong,H.Q., Liu,L., Cui,S.H., Ren,C.C., Li,H.Y.,
            Zhang,X.A., Zhang,L.D., Wei,T.X., Liu,J.J., Xing,W.Y., Fu,H. and
            Yan,S.J.
  TITLE     RNAi-mediated downregulation of asparaginase-like protein 1
            inhibits growth and promotes apoptosis of human cervical cancer
            line SiHa
  JOURNAL   Mol Med Rep 18 (1), 931-937 (2018)
   PUBMED   29767260
  REMARK    GeneRIF: ASRGL1 was closely associated with growth and apoptosis in
            cervical cancer. Therefore, ASRGL1 may be a novel, potentially
            effective anticervical cancer therapy.
REFERENCE   6  (bases 1 to 2270)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   7  (bases 1 to 2270)
  AUTHORS   Cantor,J.R., Stone,E.M., Chantranupong,L. and Georgiou,G.
  TITLE     The human asparaginase-like protein 1 hASRGL1 is an Ntn hydrolase
            with beta-aspartyl peptidase activity
  JOURNAL   Biochemistry 48 (46), 11026-11031 (2009)
   PUBMED   19839645
  REMARK    GeneRIF: ASRGL1 exhibits beta-aspartyl peptidase activity
            consistent with plant-type asparaginases. ASRGL1 is shown to be an
            Ntn hydrolase for which Thr168 serves as the essential N-terminal
            nucleophile for intramolecular processing and catalysis.
REFERENCE   8  (bases 1 to 2270)
  AUTHORS   Weidle,U.H., Evtimova,V., Alberti,S., Guerra,E., Fersis,N. and
            Kaul,S.
  TITLE     Cell growth stimulation by CRASH, an asparaginase-like protein
            overexpressed in human tumors and metastatic breast cancers
  JOURNAL   Anticancer Res 29 (4), 951-963 (2009)
   PUBMED   19414332
  REMARK    GeneRIF: Overexpression of CRASH is associated with metastatic
            breast cancer.
REFERENCE   9  (bases 1 to 2270)
  AUTHORS   Kim,J.M., Lee,K.H., Jeon,Y.J., Oh,J.H., Jeong,S.Y., Song,I.S.,
            Kim,J.M., Lee,D.S. and Kim,N.S.
  TITLE     Identification of genes related to Parkinson's disease using
            expressed sequence tags
  JOURNAL   DNA Res 13 (6), 275-286 (2006)
   PUBMED   17213182
REFERENCE   10 (bases 1 to 2270)
  AUTHORS   Bush,L.A., Herr,J.C., Wolkowicz,M., Sherman,N.E., Shore,A. and
            Flickinger,C.J.
  TITLE     A novel asparaginase-like protein is a sperm autoantigen in rats
  JOURNAL   Mol Reprod Dev 62 (2), 233-247 (2002)
   PUBMED   11984834
  REMARK    GeneRIF: Human ALP cDNA was subsequently cloned. It showed 77%
            identity to the rat ALP sequence and the gene, ASRGL1
            (asparaginase-like 1), mapped to chromosome locus 11q12.3.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA159051.1, BC093070.1, AP003064.2 and DT220454.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001083926.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK091894.1, HM005655.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000415229.6/ ENSP00000400057.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-325               DA159051.1         1-325
            326-1351            BC093070.1         297-1322
            1352-2252           AP003064.2         95642-96542
            2253-2270           DT220454.1         78-95
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.3"
     gene            1..2270
                     /gene="ASRGL1"
                     /gene_synonym="ALP; ALP1; CRASH"
                     /note="asparaginase and isoaspartyl peptidase 1"
                     /db_xref="GeneID:80150"
                     /db_xref="HGNC:HGNC:16448"
                     /db_xref="MIM:609212"
     exon            1..127
                     /gene="ASRGL1"
                     /gene_synonym="ALP; ALP1; CRASH"
                     /inference="alignment:Splign:2.1.0"
     exon            128..405
                     /gene="ASRGL1"
                     /gene_synonym="ALP; ALP1; CRASH"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..1142
                     /gene="ASRGL1"
                     /gene_synonym="ALP; ALP1; CRASH"
                     /EC_number="3.5.1.1"
                     /EC_number="3.4.19.5"
                     /note="asparaginase-like 1 protein; L-asparagine
                     amidohydrolase; L-asparaginase; asparaginase like 1;
                     asparaginase-like protein 1; beta-aspartyl-peptidase;
                     isoaspartyl dipeptidase; testis secretory sperm-binding
                     protein Li 242mP"
                     /codon_start=1
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="NP_001077395.1"
                     /db_xref="CCDS:CCDS8019.1"
                     /db_xref="GeneID:80150"
                     /db_xref="HGNC:HGNC:16448"
                     /db_xref="MIM:609212"
                     /translation="MNPIVVVHGGGAGPISKDRKERVHQGMVRAATVGYGILREGGSA
                     VDAVEGAVVALEDDPEFNAGCGSVLNTNGEVEMDASIMDGKDLSAGAVSAVQCIANPI
                     KLARLVMEKTPHCFLTDQGAAQFAAAMGVPEIPGEKLVTERNKKRLEKEKHEKGAQKT
                     DCQKNLGTVGAVALDCKGNVAYATSTGGIVNKMVGRVGDSPCLGAGGYADNDIGAVST
                     TGHGESILKVNLARLTLFHIEQGKTVEEAADLSLGYMKSRVKGLGGLIVVSKTGDWVA
                     KWTSTSMPWAAAKDGKLHFGIDPDDTTITDLP"
     exon            406..548
                     /gene="ASRGL1"
                     /gene_synonym="ALP; ALP1; CRASH"
                     /inference="alignment:Splign:2.1.0"
     exon            549..706
                     /gene="ASRGL1"
                     /gene_synonym="ALP; ALP1; CRASH"
                     /inference="alignment:Splign:2.1.0"
     exon            707..825
                     /gene="ASRGL1"
                     /gene_synonym="ALP; ALP1; CRASH"
                     /inference="alignment:Splign:2.1.0"
     exon            826..936
                     /gene="ASRGL1"
                     /gene_synonym="ALP; ALP1; CRASH"
                     /inference="alignment:Splign:2.1.0"
     exon            937..2270
                     /gene="ASRGL1"
                     /gene_synonym="ALP; ALP1; CRASH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2254..2259
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASRGL1"
                     /gene_synonym="ALP; ALP1; CRASH"
                     /note="hexamer: AATATA"
     polyA_site      2270
                     /gene="ASRGL1"
                     /gene_synonym="ALP; ALP1; CRASH"
                     /note="major polyA site"
ORIGIN      
        1 gttgtgacca gagcggttgc gggctgagcg gtttcgagcc ggcgtcgggg agcggcggta
       61 ccgggcggct gcggggctgg ctcgacccag cttgaggtct cggcgtccgc gtcctgcggt
      121 gccctggggt ctcccgagga ccttgtaccc gcgcggcttc cttgggctgg ctttggacga
      181 cgctttcgcc ttcctgctgc ctaggatccg ccgacatgaa tcccatcgta gtggtccacg
      241 gcggcggagc cggtcccatc tccaaggatc ggaaggagcg agtgcaccag ggcatggtca
      301 gagccgccac cgtgggctac ggcatcctcc gggagggcgg gagcgccgtg gatgccgtag
      361 agggagctgt cgtcgccctg gaagacgatc ccgagttcaa cgcaggttgt gggtctgtct
      421 tgaacacaaa tggtgaggtt gaaatggatg ctagtatcat ggatggaaaa gacctgtctg
      481 caggagcagt gtccgcagtc cagtgtatag caaatcccat taaacttgct cggcttgtca
      541 tggaaaagac acctcattgc tttctgactg accaaggcgc agcgcagttt gcagcagcta
      601 tgggggttcc agagattcct ggagaaaaac tggtgacaga gagaaacaaa aagcgcctgg
      661 aaaaagagaa gcatgaaaaa ggtgctcaga aaacagattg tcaaaaaaac ttgggaaccg
      721 tgggtgctgt tgccttggac tgcaaaggga atgtagccta cgcaacctcc acaggcggta
      781 tcgttaataa aatggtcggc cgcgttgggg actcaccgtg tctaggagct ggaggttatg
      841 ccgacaatga catcggagcc gtctcaacca cagggcatgg ggaaagcatc ctgaaggtga
      901 acctggctag actcaccctg ttccacatag aacaaggaaa gacggtagaa gaggctgcgg
      961 acctatcgtt gggttatatg aagtcaaggg ttaaaggttt aggtggcctc atcgtggtta
     1021 gcaaaacagg agactgggtg gcaaagtgga cctccacctc catgccctgg gcagccgcca
     1081 aggacggcaa gctgcacttc ggaattgatc ctgacgatac tactatcacc gaccttccct
     1141 aagccgctgg aagattgtat tccagatgct agcttagagg tcaagtacag tctcctcatg
     1201 agacatagcc taatcaatta gatctagaat tggaaaaatt gtcccgtctg tcacttgttt
     1261 tgttgcctta ataagcatct gaatgtttgg ttgtggggcg ggttctgaag cgatgagaga
     1321 aatgcccgta ttaggaggat tacttgagcc caggaggtca aagctgaggt gagccatgat
     1381 tactccactg cactccagcc tgggcaacag agccaggccc tgtatcaaaa aaaaaaaaaa
     1441 aaagaaaagg gaaaaaagaa agaaagcagc agcatgatcc tgacatgaca gatgtgggag
     1501 acccacagcc tgcagacact gtgggctgga aggtgggaag ggaggggccg gtggaggtgg
     1561 agctgtttga aagtgacaca gcagcagtag aagcagtggt gggcgaagcc caggtgaccc
     1621 tcagaacgtt gcacaagaac atcagggaaa agaaccagaa tcctttaagg aaaatgttct
     1681 tcatgtatga gagactaaag tgatttttct aagaaagttc agcccttctc tgacttacct
     1741 ggacatttct agatacttcc aaaggaccct ctgggaatcc atagcttcct aatctggaga
     1801 tgggaggtca taagggagac gctgtggggt tccttgaagt ttcttgggtt cacagaggag
     1861 ccccctcact tggtgttctc ccgtgagcca gcctccacct gccaaagaca ctctggtcct
     1921 cgtatagtga gtaatggggc tcagggcctc tccaacaaca gagaggagct gatgctgtag
     1981 ggctgacccc gtgacttcct gagtcctcac cctgtccagt gctttgagat tcttcccacc
     2041 tccccatcct caccagccgg atcgggcgct gtgcagtgtg gtcagcatgg tgaagaaagt
     2101 catttcctcg gtgggcagta ttcctcttta tctctcatta cactggaaat gttatttctg
     2161 ctgtatcatc cgtgctcaac gttttagtct gtcaggctca ccttctctct ggaaagaatt
     2221 tgcttaactt gacattccat gtgccgctaa taaaatatat tttgaaagaa
//
