LOCUS       NM_004318               5301 bp    mRNA    linear   PRI 17-SEP-2024
DEFINITION  Homo sapiens aspartate beta-hydroxylase (ASPH), transcript variant
            1, mRNA.
ACCESSION   NM_004318
VERSION     NM_004318.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Chen,Z.X., Jia,W.N., Sun,Y. and Jiang,Y.X.
  TITLE     Genotype-phenotype profile of global ASPH-associated ectopia lentis
            and clinical findings from a Chinese cohort
  JOURNAL   Gene 925, 148600 (2024)
   PUBMED   38788814
  REMARK    GeneRIF: Genotype-phenotype profile of global ASPH-associated
            ectopia lentis and clinical findings from a Chinese cohort.
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Zou,Y., Lin,J., Liu,J., Zhang,F., Yang,T., Gong,J., Jiang,T.,
            Zuo,J., Song,R., Shen,H., Shen,F. and Li,J.
  TITLE     Aspartate beta-hydroxylase (ASPH) Accelerates Intrahepatic
            Cholangiocarcinoma Metastasis via Upregulating SHH Signaling
            Pathway
  JOURNAL   Curr Protein Pept Sci 24 (5), 436-446 (2023)
   PUBMED   37132101
  REMARK    GeneRIF: Aspartate beta-hydroxylase (ASPH) Accelerates Intrahepatic
            Cholangiocarcinoma Metastasis via Upregulating SHH Signaling
            Pathway.
REFERENCE   3  (bases 1 to 5301)
  AUTHORS   Finotti,A., Treves,S., Zorzato,F., Gambari,R. and Feriotto,G.
  TITLE     Upstream stimulatory factors are involved in the P1 promoter
            directed transcription of the A beta H-J-J locus
  JOURNAL   BMC Mol Biol 9, 110 (2008)
   PUBMED   19087304
  REMARK    GeneRIF: USF1 and USF2 positively regulate the core of P1 promoter
            od AAH.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5301)
  AUTHORS   Treves,S., Franzini-Armstrong,C., Moccagatta,L., Arnoult,C.,
            Grasso,C., Schrum,A., Ducreux,S., Zhu,M.X., Mikoshiba,K.,
            Girard,T., Smida-Rezgui,S., Ronjat,M. and Zorzato,F.
  TITLE     Junctate is a key element in calcium entry induced by activation of
            InsP3 receptors and/or calcium store depletion
  JOURNAL   J Cell Biol 166 (4), 537-548 (2004)
   PUBMED   15302852
  REMARK    GeneRIF: junctate has a role in calcium homeostasis in eukaryotic
            cells
REFERENCE   5  (bases 1 to 5301)
  AUTHORS   Treves,S., Feriotto,G., Moccagatta,L., Gambari,R. and Zorzato,F.
  TITLE     Molecular cloning, expression, functional characterization,
            chromosomal localization, and gene structure of junctate, a novel
            integral calcium binding protein of sarco(endo)plasmic reticulum
            membrane
  JOURNAL   J Biol Chem 275 (50), 39555-39568 (2000)
   PUBMED   11007777
REFERENCE   6  (bases 1 to 5301)
  AUTHORS   Dinchuk,J.E., Henderson,N.L., Burn,T.C., Huber,R., Ho,S.P.,
            Link,J., O'Neil,K.T., Focht,R.J., Scully,M.S., Hollis,J.M.,
            Hollis,G.F. and Friedman,P.A.
  TITLE     Aspartyl beta -hydroxylase (Asph) and an evolutionarily conserved
            isoform of Asph missing the catalytic domain share exons with
            junctin
  JOURNAL   J Biol Chem 275 (50), 39543-39554 (2000)
   PUBMED   10956665
REFERENCE   7  (bases 1 to 5301)
  AUTHORS   Wetzel,G.T., Ding,S. and Chen,F.
  TITLE     Molecular cloning of junctin from human and developing rabbit heart
  JOURNAL   Mol Genet Metab 69 (3), 252-258 (2000)
   PUBMED   10767180
REFERENCE   8  (bases 1 to 5301)
  AUTHORS   Zhang,L., Kelley,J., Schmeisser,G., Kobayashi,Y.M. and Jones,L.R.
  TITLE     Complex formation between junctin, triadin, calsequestrin, and the
            ryanodine receptor. Proteins of the cardiac junctional sarcoplasmic
            reticulum membrane
  JOURNAL   J Biol Chem 272 (37), 23389-23397 (1997)
   PUBMED   9287354
REFERENCE   9  (bases 1 to 5301)
  AUTHORS   Lavaissiere,L., Jia,S., Nishiyama,M., de la Monte,S., Stern,A.M.,
            Wands,J.R. and Friedman,P.A.
  TITLE     Overexpression of human aspartyl(asparaginyl)beta-hydroxylase in
            hepatocellular carcinoma and cholangiocarcinoma
  JOURNAL   J Clin Invest 98 (6), 1313-1323 (1996)
   PUBMED   8823296
REFERENCE   10 (bases 1 to 5301)
  AUTHORS   Korioth,F., Gieffers,C. and Frey,J.
  TITLE     Cloning and characterization of the human gene encoding aspartyl
            beta-hydroxylase
  JOURNAL   Gene 150 (2), 395-399 (1994)
   PUBMED   7821814
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC067881.5, S83325.1, U03109.1
            and AC090094.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_004318.3.
            
            Summary: This gene is thought to play an important role in calcium
            homeostasis. The gene is expressed from two promoters and undergoes
            extensive alternative splicing. The encoded set of proteins share
            varying amounts of overlap near their N-termini but have
            substantial variations in their C-terminal domains resulting in
            distinct functional properties. The longest isoforms (a and f)
            include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain
            that hydroxylates aspartic acid or asparagine residues in the
            epidermal growth factor (EGF)-like domains of some proteins,
            including protein C, coagulation factors VII, IX, and X, and the
            complement factors C1R and C1S. Other isoforms differ primarily in
            the C-terminal sequence and lack the hydroxylase domain, and some
            have been localized to the endoplasmic and sarcoplasmic reticulum.
            Some of these isoforms are found in complexes with calsequestrin,
            triadin, and the ryanodine receptor, and have been shown to
            regulate calcium release from the sarcoplasmic reticulum. Some
            isoforms have been implicated in metastasis. [provided by RefSeq,
            Sep 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform. The distinct C-terminus
            of this isoform has enzymatic activity which hydroxylates the beta
            carbon of aspartic acid or asparagine residues in certain epidermal
            growth factor-like domains of proteins such as protein C,
            coagulation factors VII, IX, and X, and the complement factors C1R
            and C1S. This variant is widely expressed among heart, placenta,
            skeletal muscle, kidney, and lung tissues.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.139191.1,
                                           SRR1660803.41362.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000379454.9/ ENSP00000368767.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-210               AC067881.5         152243-152452       c
            211-367             S83325.1           1-157
            368-375             U03109.1           224-231
            376-2341            S83325.1           166-2131
            2342-2596           U03109.1           2195-2449
            2597-5301           AC090094.5         111235-113939
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q12.3"
     gene            1..5301
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /note="aspartate beta-hydroxylase"
                     /db_xref="GeneID:444"
                     /db_xref="HGNC:HGNC:757"
                     /db_xref="MIM:600582"
     exon            1..324
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     CDS             222..2498
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /EC_number="1.14.11.16"
                     /note="isoform 1 is encoded by transcript variant 1;
                     junctate; peptide-aspartate beta-dioxygenase; humbug;
                     aspartyl/asparaginyl beta-hydroxylase; cardiac junctin; A
                     beta H-J-J; ASP beta-hydroxylase"
                     /codon_start=1
                     /product="aspartyl/asparaginyl beta-hydroxylase isoform 1"
                     /protein_id="NP_004309.2"
                     /db_xref="CCDS:CCDS34898.1"
                     /db_xref="GeneID:444"
                     /db_xref="HGNC:HGNC:757"
                     /db_xref="MIM:600582"
                     /translation="MAQRKNAKSSGNSSSSGSGSGSTSAGSSSPGARRETKHGGHKNG
                     RKGGLSGTSFFTWFMVIALLGVWTSVAVVWFDLVDYEEVLGKLGIYDADGDGDFDVDD
                     AKVLLGLKERSTSEPAVPPEEAEPHTEPEEQVPVEAEPQNIEDEAKEQIQSLLHEMVH
                     AEHVEGEDLQQEDGPTGEPQQEDDEFLMATDVDDRFETLEPEVSHEETEHSYHVEETV
                     SQDCNQDMEEMMSEQENPDSSEPVVEDERLHHDTDDVTYQVYEEQAVYEPLENEGIEI
                     TEVTAPPEDNPVEDSQVIVEEVSIFPVEEQQEVPPETNRKTDDPEQKAKVKKKKPKLL
                     NKFDKTIKAELDAAEKLRKRGKIEEAVNAFKELVRKYPQSPRARYGKAQCEDDLAEKR
                     RSNEVLRGAIETYQEVASLPDVPADLLKLSLKRRSDRQQFLGHMRGSLLTLQRLVQLF
                     PNDTSLKNDLGVGYLLIGDNDNAKKVYEEVLSVTPNDGFAKVHYGFILKAQNKIAESI
                     PYLKEGIESGDPGTDDGRFYFHLGDAMQRVGNKEAYKWYELGHKRGHFASVWQRSLYN
                     VNGLKAQPWWTPKETGYTELVKSLERNWKLIRDEGLAVMDKAKGLFLPEDENLREKGD
                     WSQFTLWQQGRRNENACKGAPKTCTLLEKFPETTGCRRGQIKYSIMHPGTHVWPHTGP
                     TNCRLRMHLGLVIPKEGCKIRCANETKTWEEGKVLIFDDSFEHEVWQDASSFRLIFIV
                     DVWHPELTPQQRRSLPAI"
     exon            325..474
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            475..543
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            544..636
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            637..711
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            712..840
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            841..873
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            874..930
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            931..978
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1011
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1053
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1110
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1155
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1197
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1283
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1370
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1521
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1658
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1757
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1847
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1848..1985
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..2121
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            2122..2213
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            2214..2347
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            2348..5301
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4606..4611
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /note="hexamer: AATAAA"
     polyA_site      4628
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /note="major polyA site"
     regulatory      5278..5283
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /note="hexamer: AATAAA"
     polyA_site      5301
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
ORIGIN      
        1 agtcacaggt tcgtacacgc gaggccgggc gcgcgcagcc ggcaggcggg tgaagtcttc
       61 ccaggtgctg caggcggtgc tgaggcacag ggtctgctgc aggagcagcg gcccgaaccc
      121 gctccagcgg cccgccgccg ccagcggttc ccgcgtcgcg tgtgtacccc cgcgcactga
      181 aggaggtccg ccagccctca ccagcccccg cggaccgtgc aatggcccag cgtaagaatg
      241 ccaagagcag cggcaacagc agcagcagcg gctccggcag cggtagcacg agtgcgggca
      301 gcagcagccc cggggcccgg agagagacaa agcatggagg acacaagaat gggaggaaag
      361 gcggactctc aggaacttca ttcttcacgt ggtttatggt gattgcattg ctgggcgtct
      421 ggacatctgt agctgtcgtt tggtttgatc ttgttgacta tgaggaagtt ctaggaaaac
      481 taggaatcta tgatgctgat ggtgatggag attttgatgt ggatgatgcc aaagttttat
      541 taggacttaa agagagatct acttcagagc cagcagtccc gccagaagag gctgagccac
      601 acactgagcc cgaggagcag gttcctgtgg aggcagaacc ccagaatatc gaagatgaag
      661 caaaagaaca aattcagtcc cttctccatg aaatggtaca cgcagaacat gttgagggag
      721 aagacttgca acaagaagat ggacccacag gagaaccaca acaagaggat gatgagtttc
      781 ttatggcgac tgatgtagat gatagatttg agaccctgga acctgaagta tctcatgaag
      841 aaaccgagca tagttaccac gtggaagaga cagtttcaca agactgtaat caggatatgg
      901 aagagatgat gtctgagcag gaaaatccag attccagtga accagtagta gaagatgaaa
      961 gattgcacca tgatacagat gatgtaacat accaagtcta tgaggaacaa gcagtatatg
     1021 aacctctaga aaatgaaggg atagaaatca cagaagtaac tgctccccct gaggataatc
     1081 ctgtagaaga ttcacaggta attgtagaag aagtaagcat ttttcctgtg gaagaacagc
     1141 aggaagtacc accagaaaca aatagaaaaa cagatgatcc agaacaaaaa gcaaaagtta
     1201 agaaaaagaa gcctaaactt ttaaataaat ttgataagac tattaaagct gaacttgatg
     1261 ctgcagaaaa actccgtaaa aggggaaaaa ttgaggaagc agtgaatgca tttaaagaac
     1321 tagtacgcaa ataccctcag agtccacgag caagatatgg gaaggcgcag tgtgaggatg
     1381 atttggctga gaagaggaga agtaatgagg tgctacgtgg agccatcgag acctaccaag
     1441 aggtggccag cctacctgat gtccctgcag acctgctgaa gctgagtttg aagcgtcgct
     1501 cagacaggca acaatttcta ggtcatatga gaggttccct gcttaccctg cagagattag
     1561 ttcaactatt tcccaatgat acttccttaa aaaatgacct tggcgtggga tacctcttga
     1621 taggagataa tgacaatgca aagaaagttt atgaagaggt gctgagtgtg acacctaatg
     1681 atggctttgc taaagtccat tatggcttca tcctgaaggc acagaacaaa attgctgaga
     1741 gcatcccata tttaaaggaa ggaatagaat ccggagatcc tggcactgat gatgggagat
     1801 tttatttcca cctgggggat gccatgcaga gggttgggaa caaagaggca tataagtggt
     1861 atgagcttgg gcacaagaga ggacactttg catctgtctg gcaacgctca ctctacaatg
     1921 tgaatggact gaaagcacag ccttggtgga ccccaaaaga aacgggctac acagagttag
     1981 taaagtcttt agaaagaaac tggaagttaa tccgagatga aggccttgca gtgatggata
     2041 aagccaaagg tctcttcctg cctgaggatg aaaacctgag ggaaaaaggg gactggagcc
     2101 agttcacgct gtggcagcaa ggaagaagaa atgaaaatgc ctgcaaagga gctcctaaaa
     2161 cctgtacctt actagaaaag ttccccgaga caacaggatg cagaagagga cagatcaaat
     2221 attccatcat gcaccccggg actcacgtgt ggccgcacac agggcccaca aactgcaggc
     2281 tccgaatgca cctgggcttg gtgattccca aggaaggctg caagattcga tgtgccaacg
     2341 agaccaagac ctgggaggaa ggcaaggtgc tcatctttga tgactccttt gagcacgagg
     2401 tatggcagga tgcctcatct ttccggctga tattcatcgt ggatgtgtgg catccggaac
     2461 tgacaccaca gcagagacgc agccttccag caatttagca tgaattcatg caagcttggg
     2521 aaactctgga gagaggctgc ctttctggtt ccatctcctt gggtgtgagg atagaatttc
     2581 gaacaccaag agtcaattcc cttgacttgc agcccgagta attcaaagcc tcctcctagg
     2641 gtcagaagac actaaaggga atatttgcct cgctgcaatt catttaggaa acaccctgct
     2701 gtgtgtcatc tcatgacagc actggtcttc tgccagtatt taaggtgaac atttgatagc
     2761 ttctacctta ccagccaaag atattttttc cacatagaat aggtctaatt caatgtataa
     2821 tgagaacata tgtagaaact gtgaatggat tgctttagtt tgtaattttt ctatgcagtt
     2881 atatttttct agtgtagcta gactattttg tcatcatgta ccactacatt tttgtttatt
     2941 ttaatgacaa gctgtataaa tgctttactt ctagctattt aatggtagca ttactgggga
     3001 actcagactt ccctctttta attcttctta gtaaaagata ctcatgaaaa aagcagtttt
     3061 attttcctaa caaaaaagaa agagctcatt atgtcagtgt ctatgaactg tacccatccc
     3121 aactctcaaa tcgtttggtt ttttttatct tgattgagat cctcttctca ctatgctagt
     3181 ggtggagata ttgacaaaat cctatttctt tcaaagagga acttttcaca ccgaaaaaag
     3241 agcatggaat tattttatat tgttataaaa atcccagatg caaatttttt taatgccaat
     3301 tattagagct tctggggaaa aagtatagtt cacggaaata aaactatgtt ctttcagggt
     3361 tgggtggata ggtggctgct agggtgtctg gctcctggcg gctttgccat ccatgaggca
     3421 agggctggga acacagtgtc tttgcctatg gtagatccat gtgaatgtca ggaagccagc
     3481 tcttcagtct tggagatgat ttctgctaca attctgtaga aagattaagg atggcagagt
     3541 aaaaggttac caagaatgcc aggatgtttt tcttgggcgt aggaggtcca gattactttc
     3601 ctttttgatg aaagagtttg gaagactgtc ccatctctct ggcttgagaa atctctgcca
     3661 ttttaaacat cactgtgaaa tagcaattat tatcatctgt atttagtttt aacattaccc
     3721 acaacataga aataataggt aaaaatcgtc ttgcctactc attccaaaga tgatcaagtc
     3781 attaatctag caaagtattc atgtatcaga ttttgtatat tttgaatcaa agctaactag
     3841 gaatgttaga tataagaatg taatgatatt catgcactga attctaagcc aatatgaaca
     3901 aaaatgctgc atgaatggca catataggtc accaaagttc attcacaggt agaaaaaact
     3961 tgtgctttct tttccatcta aaaacaaaag gagactttct ttatctcatt taaagaacag
     4021 ctctttgaaa ttgaaattga ccctttttgc ttgaccttaa ggagattagc ttccagtaga
     4081 tgagtttgca aaatactttt cctgttcttt tgttttgctg gtattgaaaa catcccacta
     4141 aatcagatga agaggcatgg gaggaaaaat atccaaatta attactaaaa tcgagaagag
     4201 aaggcaaact cttgaaaagt aaaaaggtgt ttgtgacctt cagtatttat tgaacagagg
     4261 aaataactga caagggcaat acaattcaat gttcatgtag taacattcat gtcacttgtt
     4321 gaatttggtt ctcatatgta tattgcatac acataaattc aaactataag tcgtcatttt
     4381 tgagccatca tcttacattc atgtaatgaa attatggaag agagtaaaaa ctagctctta
     4441 acttagtaaa tataatatgg tatttaaaat caggtcacta cagtaaggtt ctaagtattg
     4501 ccaattgaaa agctagaaat ggtattactg ttgcaaagtg ttgtcaataa ttgactccaa
     4561 tagcattgta aatacttgta tcccacaact attttaaacc caagcaataa aatggatttt
     4621 ctaattcact tcaattcttt atttctctta cctatctatg tcttggtaca taagaaaagt
     4681 gtttccatca ctgcattggt agaattattt cagtgttatt atttttgtga tttcgtatgt
     4741 ctacacaaaa atgaattagt ttaatttata ttgtgataca gttgtttgag aaatattttt
     4801 aattctgttt caactttatt tctccaagtg tataatataa aaattacttc tgttattgtt
     4861 ctctaccaat aggggaaaaa attaaaacat tagatccatt gagaaagaga tgatgtaata
     4921 aataattaag attagtaata atattatcag gggtgattat gaccagttga ataatctctt
     4981 tcccttgaat tatttagcta acaaattaac tctcccaaat atttaaaata atgtaaaatc
     5041 atattttact gcccattatt aactaaaata tttttgtttg actttgagca ccaactggta
     5101 atactaataa atacccatgt catgcagatg gctgggcgaa taagagatgt ctaaaaatat
     5161 gcactggtct tggaaaacat ggcacaagta aggatatcat atatgatgtc tgtttatttt
     5221 atgtctgatt tcttttgaat gagtagttgg ggactccatt tctaaggaga ctaggtaaat
     5281 aaaatgacct ttgacatttc a
//
