LOCUS       NM_001413894            5040 bp    mRNA    linear   PRI 17-SEP-2024
DEFINITION  Homo sapiens aspartate beta-hydroxylase (ASPH), transcript variant
            62, mRNA.
ACCESSION   NM_001413894
VERSION     NM_001413894.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Chen,Z.X., Jia,W.N., Sun,Y. and Jiang,Y.X.
  TITLE     Genotype-phenotype profile of global ASPH-associated ectopia lentis
            and clinical findings from a Chinese cohort
  JOURNAL   Gene 925, 148600 (2024)
   PUBMED   38788814
  REMARK    GeneRIF: Genotype-phenotype profile of global ASPH-associated
            ectopia lentis and clinical findings from a Chinese cohort.
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Zou,Y., Lin,J., Liu,J., Zhang,F., Yang,T., Gong,J., Jiang,T.,
            Zuo,J., Song,R., Shen,H., Shen,F. and Li,J.
  TITLE     Aspartate beta-hydroxylase (ASPH) Accelerates Intrahepatic
            Cholangiocarcinoma Metastasis via Upregulating SHH Signaling
            Pathway
  JOURNAL   Curr Protein Pept Sci 24 (5), 436-446 (2023)
   PUBMED   37132101
  REMARK    GeneRIF: Aspartate beta-hydroxylase (ASPH) Accelerates Intrahepatic
            Cholangiocarcinoma Metastasis via Upregulating SHH Signaling
            Pathway.
REFERENCE   3  (bases 1 to 5040)
  AUTHORS   Finotti,A., Treves,S., Zorzato,F., Gambari,R. and Feriotto,G.
  TITLE     Upstream stimulatory factors are involved in the P1 promoter
            directed transcription of the A beta H-J-J locus
  JOURNAL   BMC Mol Biol 9, 110 (2008)
   PUBMED   19087304
  REMARK    GeneRIF: USF1 and USF2 positively regulate the core of P1 promoter
            od AAH.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5040)
  AUTHORS   Treves,S., Franzini-Armstrong,C., Moccagatta,L., Arnoult,C.,
            Grasso,C., Schrum,A., Ducreux,S., Zhu,M.X., Mikoshiba,K.,
            Girard,T., Smida-Rezgui,S., Ronjat,M. and Zorzato,F.
  TITLE     Junctate is a key element in calcium entry induced by activation of
            InsP3 receptors and/or calcium store depletion
  JOURNAL   J Cell Biol 166 (4), 537-548 (2004)
   PUBMED   15302852
  REMARK    GeneRIF: junctate has a role in calcium homeostasis in eukaryotic
            cells
REFERENCE   5  (bases 1 to 5040)
  AUTHORS   Treves,S., Feriotto,G., Moccagatta,L., Gambari,R. and Zorzato,F.
  TITLE     Molecular cloning, expression, functional characterization,
            chromosomal localization, and gene structure of junctate, a novel
            integral calcium binding protein of sarco(endo)plasmic reticulum
            membrane
  JOURNAL   J Biol Chem 275 (50), 39555-39568 (2000)
   PUBMED   11007777
REFERENCE   6  (bases 1 to 5040)
  AUTHORS   Dinchuk,J.E., Henderson,N.L., Burn,T.C., Huber,R., Ho,S.P.,
            Link,J., O'Neil,K.T., Focht,R.J., Scully,M.S., Hollis,J.M.,
            Hollis,G.F. and Friedman,P.A.
  TITLE     Aspartyl beta -hydroxylase (Asph) and an evolutionarily conserved
            isoform of Asph missing the catalytic domain share exons with
            junctin
  JOURNAL   J Biol Chem 275 (50), 39543-39554 (2000)
   PUBMED   10956665
REFERENCE   7  (bases 1 to 5040)
  AUTHORS   Wetzel,G.T., Ding,S. and Chen,F.
  TITLE     Molecular cloning of junctin from human and developing rabbit heart
  JOURNAL   Mol Genet Metab 69 (3), 252-258 (2000)
   PUBMED   10767180
REFERENCE   8  (bases 1 to 5040)
  AUTHORS   Zhang,L., Kelley,J., Schmeisser,G., Kobayashi,Y.M. and Jones,L.R.
  TITLE     Complex formation between junctin, triadin, calsequestrin, and the
            ryanodine receptor. Proteins of the cardiac junctional sarcoplasmic
            reticulum membrane
  JOURNAL   J Biol Chem 272 (37), 23389-23397 (1997)
   PUBMED   9287354
REFERENCE   9  (bases 1 to 5040)
  AUTHORS   Lavaissiere,L., Jia,S., Nishiyama,M., de la Monte,S., Stern,A.M.,
            Wands,J.R. and Friedman,P.A.
  TITLE     Overexpression of human aspartyl(asparaginyl)beta-hydroxylase in
            hepatocellular carcinoma and cholangiocarcinoma
  JOURNAL   J Clin Invest 98 (6), 1313-1323 (1996)
   PUBMED   8823296
REFERENCE   10 (bases 1 to 5040)
  AUTHORS   Korioth,F., Gieffers,C. and Frey,J.
  TITLE     Cloning and characterization of the human gene encoding aspartyl
            beta-hydroxylase
  JOURNAL   Gene 150 (2), 395-399 (1994)
   PUBMED   7821814
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC067881.5 and AC090094.5.
            
            Summary: This gene is thought to play an important role in calcium
            homeostasis. The gene is expressed from two promoters and undergoes
            extensive alternative splicing. The encoded set of proteins share
            varying amounts of overlap near their N-termini but have
            substantial variations in their C-terminal domains resulting in
            distinct functional properties. The longest isoforms (a and f)
            include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain
            that hydroxylates aspartic acid or asparagine residues in the
            epidermal growth factor (EGF)-like domains of some proteins,
            including protein C, coagulation factors VII, IX, and X, and the
            complement factors C1R and C1S. Other isoforms differ primarily in
            the C-terminal sequence and lack the hydroxylase domain, and some
            have been localized to the endoplasmic and sarcoplasmic reticulum.
            Some of these isoforms are found in complexes with calsequestrin,
            triadin, and the ryanodine receptor, and have been shown to
            regulate calcium release from the sarcoplasmic reticulum. Some
            isoforms have been implicated in metastasis. [provided by RefSeq,
            Sep 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.332135.1,
                                           SRR14038192.3268490.1 [ECO:0000332]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-324               AC067881.5         152129-152452       c
            325-474             AC067881.5         121899-122048       c
            475-543             AC067881.5         118828-118896       c
            544-636             AC067881.5         91428-91520         c
            637-669             AC067881.5         82460-82492         c
            670-717             AC067881.5         81246-81293         c
            718-750             AC067881.5         80748-80780         c
            751-792             AC067881.5         76182-76223         c
            793-849             AC067881.5         75807-75863         c
            850-894             AC067881.5         71543-71587         c
            895-936             AC067881.5         56838-56879         c
            937-1022            AC090094.5         30466-30551
            1023-1109           AC090094.5         37637-37723
            1110-1260           AC090094.5         47177-47327
            1261-1397           AC090094.5         51615-51751
            1398-1496           AC090094.5         58473-58571
            1497-1586           AC090094.5         61375-61464
            1587-1724           AC090094.5         66287-66424
            1725-1860           AC090094.5         88383-88518
            1861-1952           AC090094.5         96372-96463
            1953-2086           AC090094.5         96834-96967
            2087-5040           AC090094.5         110986-113939
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q12.3"
     gene            1..5040
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /note="aspartate beta-hydroxylase"
                     /db_xref="GeneID:444"
                     /db_xref="HGNC:HGNC:757"
                     /db_xref="MIM:600582"
     exon            1..324
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     CDS             222..2237
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /EC_number="1.14.11.16"
                     /note="isoform 50 is encoded by transcript variant 62;
                     junctate; peptide-aspartate beta-dioxygenase; humbug;
                     aspartyl/asparaginyl beta-hydroxylase; cardiac junctin; A
                     beta H-J-J; ASP beta-hydroxylase"
                     /codon_start=1
                     /product="aspartyl/asparaginyl beta-hydroxylase isoform
                     50"
                     /protein_id="NP_001400823.1"
                     /db_xref="GeneID:444"
                     /db_xref="HGNC:HGNC:757"
                     /db_xref="MIM:600582"
                     /translation="MAQRKNAKSSGNSSSSGSGSGSTSAGSSSPGARRETKHGGHKNG
                     RKGGLSGTSFFTWFMVIALLGVWTSVAVVWFDLVDYEEVLGKLGIYDADGDGDFDVDD
                     AKVLLGLKERSTSEPAVPPEEAEPHTEPEEQVPVEAETEHSYHVEETDSSEPVVEDER
                     LHHDTDDVTYQVYEEQAVYEPLENEGIEITEVTAPPEDNPVEDSQVIVEEVSIFPVEE
                     QQEVPPETNRKTDDPEQKAKVKKKKPKLLNKFDKTIKAELDAAEKLRKRGKIEEAVNA
                     FKELVRKYPQSPRARYGKAQCEDDLAEKRRSNEVLRGAIETYQEVASLPDVPADLLKL
                     SLKRRSDRQQFLGHMRGSLLTLQRLVQLFPNDTSLKNDLGVGYLLIGDNDNAKKVYEE
                     VLSVTPNDGFAKVHYGFILKAQNKIAESIPYLKEGIESGDPGTDDGRFYFHLGDAMQR
                     VGNKEAYKWYELGHKRGHFASVWQRSLYNVNGLKAQPWWTPKETGYTELVKSLERNWK
                     LIRDEGLAVMDKAKGLFLPEDENLREKGDWSQFTLWQQGRRNENACKGAPKTCTLLEK
                     FPETTGCRRGQIKYSIMHPGTHVWPHTGPTNCRLRMHLGLVIPKEGCKIRCANETKTW
                     EEGKVLIFDDSFEHEVWQDASSFRLIFIVDVWHPELTPQQRRSLPAI"
     exon            325..474
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            475..543
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            544..636
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            637..669
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            670..717
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            718..750
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            751..792
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            793..849
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            850..894
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            895..936
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1022
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1109
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1260
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1397
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1398..1496
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1497..1586
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1587..1724
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1725..1860
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1861..1952
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            1953..2086
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     exon            2087..5040
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4345..4350
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /note="hexamer: AATAAA"
     polyA_site      4367
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /note="major polyA site"
     regulatory      5017..5022
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
                     /note="hexamer: AATAAA"
     polyA_site      5040
                     /gene="ASPH"
                     /gene_synonym="AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;
                     junctin"
ORIGIN      
        1 agtcacaggt tcgtacacgc gaggccgggc gcgcgcagcc ggcaggcggg tgaagtcttc
       61 ccaggtgctg caggcggtgc tgaggcacag ggtctgctgc aggagcagcg gcccgaaccc
      121 gctccagcgg cccgccgccg ccagcggttc ccgcgtcgcg tgtgtacccc cgcgcactga
      181 aggaggtccg ccagccctca ccagcccccg cggaccgtgc aatggcccag cgtaagaatg
      241 ccaagagcag cggcaacagc agcagcagcg gctccggcag cggtagcacg agtgcgggca
      301 gcagcagccc cggggcccgg agagagacaa agcatggagg acacaagaat gggaggaaag
      361 gcggactctc aggaacttca ttcttcacgt ggtttatggt gattgcattg ctgggcgtct
      421 ggacatctgt agctgtcgtt tggtttgatc ttgttgacta tgaggaagtt ctaggaaaac
      481 taggaatcta tgatgctgat ggtgatggag attttgatgt ggatgatgcc aaagttttat
      541 taggacttaa agagagatct acttcagagc cagcagtccc gccagaagag gctgagccac
      601 acactgagcc cgaggagcag gttcctgtgg aggcagaaac cgagcatagt taccacgtgg
      661 aagagacaga ttccagtgaa ccagtagtag aagatgaaag attgcaccat gatacagatg
      721 atgtaacata ccaagtctat gaggaacaag cagtatatga acctctagaa aatgaaggga
      781 tagaaatcac agaagtaact gctccccctg aggataatcc tgtagaagat tcacaggtaa
      841 ttgtagaaga agtaagcatt tttcctgtgg aagaacagca ggaagtacca ccagaaacaa
      901 atagaaaaac agatgatcca gaacaaaaag caaaagttaa gaaaaagaag cctaaacttt
      961 taaataaatt tgataagact attaaagctg aacttgatgc tgcagaaaaa ctccgtaaaa
     1021 ggggaaaaat tgaggaagca gtgaatgcat ttaaagaact agtacgcaaa taccctcaga
     1081 gtccacgagc aagatatggg aaggcgcagt gtgaggatga tttggctgag aagaggagaa
     1141 gtaatgaggt gctacgtgga gccatcgaga cctaccaaga ggtggccagc ctacctgatg
     1201 tccctgcaga cctgctgaag ctgagtttga agcgtcgctc agacaggcaa caatttctag
     1261 gtcatatgag aggttccctg cttaccctgc agagattagt tcaactattt cccaatgata
     1321 cttccttaaa aaatgacctt ggcgtgggat acctcttgat aggagataat gacaatgcaa
     1381 agaaagttta tgaagaggtg ctgagtgtga cacctaatga tggctttgct aaagtccatt
     1441 atggcttcat cctgaaggca cagaacaaaa ttgctgagag catcccatat ttaaaggaag
     1501 gaatagaatc cggagatcct ggcactgatg atgggagatt ttatttccac ctgggggatg
     1561 ccatgcagag ggttgggaac aaagaggcat ataagtggta tgagcttggg cacaagagag
     1621 gacactttgc atctgtctgg caacgctcac tctacaatgt gaatggactg aaagcacagc
     1681 cttggtggac cccaaaagaa acgggctaca cagagttagt aaagtcttta gaaagaaact
     1741 ggaagttaat ccgagatgaa ggccttgcag tgatggataa agccaaaggt ctcttcctgc
     1801 ctgaggatga aaacctgagg gaaaaagggg actggagcca gttcacgctg tggcagcaag
     1861 gaagaagaaa tgaaaatgcc tgcaaaggag ctcctaaaac ctgtacctta ctagaaaagt
     1921 tccccgagac aacaggatgc agaagaggac agatcaaata ttccatcatg caccccggga
     1981 ctcacgtgtg gccgcacaca gggcccacaa actgcaggct ccgaatgcac ctgggcttgg
     2041 tgattcccaa ggaaggctgc aagattcgat gtgccaacga gaccaagacc tgggaggaag
     2101 gcaaggtgct catctttgat gactcctttg agcacgaggt atggcaggat gcctcatctt
     2161 tccggctgat attcatcgtg gatgtgtggc atccggaact gacaccacag cagagacgca
     2221 gccttccagc aatttagcat gaattcatgc aagcttggga aactctggag agaggctgcc
     2281 tttctggttc catctccttg ggtgtgagga tagaatttcg aacaccaaga gtcaattccc
     2341 ttgacttgca gcccgagtaa ttcaaagcct cctcctaggg tcagaagaca ctaaagggaa
     2401 tatttgcctc gctgcaattc atttaggaaa caccctgctg tgtgtcatct catgacagca
     2461 ctggtcttct gccagtattt aaggtgaaca tttgatagct tctaccttac cagccaaaga
     2521 tattttttcc acatagaata ggtctaattc aatgtataat gagaacatat gtagaaactg
     2581 tgaatggatt gctttagttt gtaatttttc tatgcagtta tatttttcta gtgtagctag
     2641 actattttgt catcatgtac cactacattt ttgtttattt taatgacaag ctgtataaat
     2701 gctttacttc tagctattta atggtagcat tactggggaa ctcagacttc cctcttttaa
     2761 ttcttcttag taaaagatac tcatgaaaaa agcagtttta ttttcctaac aaaaaagaaa
     2821 gagctcatta tgtcagtgtc tatgaactgt acccatccca actctcaaat cgtttggttt
     2881 tttttatctt gattgagatc ctcttctcac tatgctagtg gtggagatat tgacaaaatc
     2941 ctatttcttt caaagaggaa cttttcacac cgaaaaaaga gcatggaatt attttatatt
     3001 gttataaaaa tcccagatgc aaattttttt aatgccaatt attagagctt ctggggaaaa
     3061 agtatagttc acggaaataa aactatgttc tttcagggtt gggtggatag gtggctgcta
     3121 gggtgtctgg ctcctggcgg ctttgccatc catgaggcaa gggctgggaa cacagtgtct
     3181 ttgcctatgg tagatccatg tgaatgtcag gaagccagct cttcagtctt ggagatgatt
     3241 tctgctacaa ttctgtagaa agattaagga tggcagagta aaaggttacc aagaatgcca
     3301 ggatgttttt cttgggcgta ggaggtccag attactttcc tttttgatga aagagtttgg
     3361 aagactgtcc catctctctg gcttgagaaa tctctgccat tttaaacatc actgtgaaat
     3421 agcaattatt atcatctgta tttagtttta acattaccca caacatagaa ataataggta
     3481 aaaatcgtct tgcctactca ttccaaagat gatcaagtca ttaatctagc aaagtattca
     3541 tgtatcagat tttgtatatt ttgaatcaaa gctaactagg aatgttagat ataagaatgt
     3601 aatgatattc atgcactgaa ttctaagcca atatgaacaa aaatgctgca tgaatggcac
     3661 atataggtca ccaaagttca ttcacaggta gaaaaaactt gtgctttctt ttccatctaa
     3721 aaacaaaagg agactttctt tatctcattt aaagaacagc tctttgaaat tgaaattgac
     3781 cctttttgct tgaccttaag gagattagct tccagtagat gagtttgcaa aatacttttc
     3841 ctgttctttt gttttgctgg tattgaaaac atcccactaa atcagatgaa gaggcatggg
     3901 aggaaaaata tccaaattaa ttactaaaat cgagaagaga aggcaaactc ttgaaaagta
     3961 aaaaggtgtt tgtgaccttc agtatttatt gaacagagga aataactgac aagggcaata
     4021 caattcaatg ttcatgtagt aacattcatg tcacttgttg aatttggttc tcatatgtat
     4081 attgcataca cataaattca aactataagt cgtcattttt gagccatcat cttacattca
     4141 tgtaatgaaa ttatggaaga gagtaaaaac tagctcttaa cttagtaaat ataatatggt
     4201 atttaaaatc aggtcactac agtaaggttc taagtattgc caattgaaaa gctagaaatg
     4261 gtattactgt tgcaaagtgt tgtcaataat tgactccaat agcattgtaa atacttgtat
     4321 cccacaacta ttttaaaccc aagcaataaa atggattttc taattcactt caattcttta
     4381 tttctcttac ctatctatgt cttggtacat aagaaaagtg tttccatcac tgcattggta
     4441 gaattatttc agtgttatta tttttgtgat ttcgtatgtc tacacaaaaa tgaattagtt
     4501 taatttatat tgtgatacag ttgtttgaga aatattttta attctgtttc aactttattt
     4561 ctccaagtgt ataatataaa aattacttct gttattgttc tctaccaata ggggaaaaaa
     4621 ttaaaacatt agatccattg agaaagagat gatgtaataa ataattaaga ttagtaataa
     4681 tattatcagg ggtgattatg accagttgaa taatctcttt cccttgaatt atttagctaa
     4741 caaattaact ctcccaaata tttaaaataa tgtaaaatca tattttactg cccattatta
     4801 actaaaatat ttttgtttga ctttgagcac caactggtaa tactaataaa tacccatgtc
     4861 atgcagatgg ctgggcgaat aagagatgtc taaaaatatg cactggtctt ggaaaacatg
     4921 gcacaagtaa ggatatcata tatgatgtct gtttatttta tgtctgattt cttttgaatg
     4981 agtagttggg gactccattt ctaaggagac taggtaaata aaatgacctt tgacatttca
//
