LOCUS       NM_001080464            4831 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens asparaginase (ASPG), transcript variant 1, mRNA.
ACCESSION   NM_001080464 XM_370777
VERSION     NM_001080464.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Cui,J., Jiang,L., Xu,B. and Bai,Y.
  TITLE     L-asparaginase activity and anti-L-asparaginase antibody as
            biomarkers in estimating PEG-asp-related anaphylaxis risk in
            childhood acute lymphoblastic leukemia patients
  JOURNAL   Allergol Immunopathol (Madr) 51 (3), 28-35 (2023)
   PUBMED   37169557
  REMARK    GeneRIF: L-asparaginase activity and anti-L-asparaginase antibody
            as biomarkers in estimating PEG-asp-related anaphylaxis risk in
            childhood acute lymphoblastic leukemia patients.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Van Trimpont,M., Schalk,A.M., De Visser,Y., Nguyen,H.A., Reunes,L.,
            Vandemeulebroecke,K., Peeters,E., Su,Y., Lee,H., Lorenzi,P.L.,
            Chan,W.K., Mondelaers,V., De Moerloose,B., Lammens,T., Goossens,S.,
            Van Vlierberghe,P. and Lavie,A.
  TITLE     In vivo stabilization of a less toxic asparaginase variant leads to
            a durable antitumor response in acute leukemia
  JOURNAL   Haematologica 108 (2), 409-419 (2023)
   PUBMED   35979719
  REMARK    GeneRIF: In vivo stabilization of a less toxic asparaginase variant
            leads to a durable antitumor response in acute leukemia.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4831)
  AUTHORS   Guimaraes,A.V.F., Frota,N.F. and Lourenzoni,M.R.
  TITLE     Molecular dynamics simulations of human L-asparaginase1: Insights
            into structural determinants of enzymatic activity
  JOURNAL   J Mol Graph Model 109, 108007 (2021)
   PUBMED   34461521
  REMARK    GeneRIF: Molecular dynamics simulations of human L-asparaginase1:
            Insights into structural determinants of enzymatic activity.
REFERENCE   4  (bases 1 to 4831)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 4831)
  AUTHORS   Malhotra,P., Jain,S. and Kapoor,G.
  TITLE     Symptomatic Cerebral Sinovenous Thrombosis Associated With
            L-Asparaginase In Children With Acute Lymphoblastic Leukemia: A
            Single Institution Experience Over 17 Years
  JOURNAL   J Pediatr Hematol Oncol 40 (7), e450-e453 (2018)
   PUBMED   29554021
  REMARK    GeneRIF: Incidence of symptomatic L-asparaginase associated
            Cerebral Sinovenous Thrombosis during ALL treatment was 1.5% with
            high case fatality rate (28%).
REFERENCE   6  (bases 1 to 4831)
  AUTHORS   Nomme,J., Su,Y. and Lavie,A.
  TITLE     Elucidation of the specific function of the conserved threonine
            triad responsible for human L-asparaginase autocleavage and
            substrate hydrolysis
  JOURNAL   J Mol Biol 426 (13), 2471-2485 (2014)
   PUBMED   24768817
  REMARK    GeneRIF: To determine the individual contribution of each of the
            three conserved active-site threonines (threonine triad Thr168,
            Thr186, Thr219) for the enzyme-activating autocleavage and
            asparaginase reactions, we prepared the T168S, T186V and T219A/V
            mutants.
REFERENCE   7  (bases 1 to 4831)
  AUTHORS   Shin,S.Y., Fauman,E.B., Petersen,A.K., Krumsiek,J., Santos,R.,
            Huang,J., Arnold,M., Erte,I., Forgetta,V., Yang,T.P., Walter,K.,
            Menni,C., Chen,L., Vasquez,L., Valdes,A.M., Hyde,C.L., Wang,V.,
            Ziemek,D., Roberts,P., Xi,L., Grundberg,E., Waldenberger,M.,
            Richards,J.B., Mohney,R.P., Milburn,M.V., John,S.L., Trimmer,J.,
            Theis,F.J., Overington,J.P., Suhre,K., Brosnan,M.J., Gieger,C.,
            Kastenmuller,G., Spector,T.D. and Soranzo,N.
  CONSRTM   Multiple Tissue Human Expression Resource (MuTHER) Consortium
  TITLE     An atlas of genetic influences on human blood metabolites
  JOURNAL   Nat Genet 46 (6), 543-550 (2014)
   PUBMED   24816252
REFERENCE   8  (bases 1 to 4831)
  AUTHORS   Karamitros,C.S. and Konrad,M.
  TITLE     Human 60-kDa lysophospholipase contains an N-terminal
            L-asparaginase domain that is allosterically regulated by
            L-asparagine
  JOURNAL   J Biol Chem 289 (19), 12962-12975 (2014)
   PUBMED   24657844
  REMARK    GeneRIF: Human 60-kDa lysophospholipase contains an N-terminal
            L-asparaginase domain that is allosterically regulated by
            L-asparagine.
REFERENCE   9  (bases 1 to 4831)
  AUTHORS   Nomme,J., Su,Y., Konrad,M. and Lavie,A.
  TITLE     Structures of apo and product-bound human L-asparaginase: insights
            into the mechanism of autoproteolysis and substrate hydrolysis
  JOURNAL   Biochemistry 51 (34), 6816-6826 (2012)
   PUBMED   22861376
  REMARK    GeneRIF: In the cleaved state, Thr168 of L-asparaginase directly
            activates its hydroxyl group (distance of 2.9 A).
REFERENCE   10 (bases 1 to 4831)
  AUTHORS   Menniti,M., Iuliano,R., Foller,M., Sopjani,M., Alesutan,I.,
            Mariggio,S., Nofziger,C., Perri,A.M., Amato,R., Blazer-Yost,B.,
            Corda,D., Lang,F. and Perrotti,N.
  TITLE     60kDa lysophospholipase, a new Sgk1 molecular partner involved in
            the regulation of ENaC
  JOURNAL   Cell Physiol Biochem 26 (4-5), 587-596 (2010)
   PUBMED   21063096
  REMARK    GeneRIF: The 60kDa lysophospholipase downregulates basal and
            Sgk1-dependent ENaC activity.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX407951.2, BX280385.1, BC136637.1, BM544197.1, AW024833.1 and
            AL136001.6.
            
            On Nov 22, 2018 this sequence version replaced NM_001080464.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC136637.1, DRR138522.1476326.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000551177.6/ ENSP00000450040.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                BX407951.2         15-32
            19-44               BX280385.1         8-33
            45-1102             BC136637.1         1-1058
            1103-1204           BM544197.1         659-760
            1205-1817           BC136637.1         1161-1773
            1818-1958           AW024833.1         1-141               c
            1959-4831           AL136001.6         178712-181584
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.33"
     gene            1..4831
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /note="asparaginase"
                     /db_xref="GeneID:374569"
                     /db_xref="HGNC:HGNC:20123"
                     /db_xref="MIM:618472"
     exon            1..153
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..1793
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.1.47"
                     /EC_number="3.5.1.1"
                     /note="isoform 1 is encoded by transcript variant 1; 60
                     kDa lysophospholipase; wild-type L-asparaginase;
                     asparaginase homolog; lysophospholipase-transacylase; PAF
                     acetylhydrolase"
                     /codon_start=1
                     /product="60 kDa lysophospholipase isoform 1"
                     /protein_id="NP_001073933.2"
                     /db_xref="CCDS:CCDS45170.2"
                     /db_xref="GeneID:374569"
                     /db_xref="HGNC:HGNC:20123"
                     /db_xref="MIM:618472"
                     /translation="MARAVGPERRLLAVYTGGTIGMRSELGVLVPGTGLAAILRTLPM
                     FHDEEHARARGLSEDTLVLPPASRNQRILYTVLECQPLFDSSDMTIAEWVCLAQTIKR
                     HYEQYHGFVVIHGTDTMAFAASMLSFMLENLQKTVILTGAQVPIHALWSDGRENLLGA
                     LLMAGQYVIPEVCLFFQNQLFRGNRATKVDARRFAAFCSPNLLPLATVGADITINREL
                     VRKVDGKAGLVVHSSMEQDVGLLRLYPGIPAALVRAFLQPPLKGVVMETFGSGNGPTK
                     PDLLQELRVATERGLVIVNCTHCLQGAVTTDYAAGMAMAGAGVISGFDMTSEAALAKL
                     SYVLGQPGLSLDVRKELLTKDLRGEMTPPSVEERRPSLQGNTLGGGVSWLLSLSGSQE
                     ADALRNALVPSLACAAAHAGDVEALQALVELGSDLGLVDFNGQTPLHAAARGGHTEAV
                     TMLLQRGVDVNTRDTDGFSPLLLAVRGRHPGVIGLLREAGASLSTQELEEAGTELCRL
                     AYRADLEGLQVWWQAGADLGQPGYDGHSALHVAEAAGNLAVVAFLQSLEGAVGAQAPC
                     PEVLPGV"
     exon            154..262
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     exon            263..374
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     exon            375..500
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     exon            501..584
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     exon            585..711
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     exon            712..824
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     exon            825..1007
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1121
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1122..1244
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1340
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1504
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1505..1591
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..1691
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1772
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1773..4831
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1935..1940
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /note="hexamer: AATAAA"
     polyA_site      1958
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /note="major polyA site"
     regulatory      4813..4818
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /note="hexamer: AATAAA"
     polyA_site      4831
                     /gene="ASPG"
                     /gene_synonym="C14orf76; GPA/WT; hASNase1; LYSOLP"
                     /note="major polyA site"
ORIGIN      
        1 agtccctgag tcccgcaggc cctgcgtccc cgctgcacac ccccgtccac tcccgtggtc
       61 cccggtccgg catggcgcgc gcggtggggc ccgagcggag gctgctggcc gtctacaccg
      121 gcggcaccat tggcatgcgg agtgagctcg gcgtgcttgt gcccgggacg ggcctggctg
      181 ccatcctgag gacactgccc atgttccatg acgaggagca cgcccgagcc cgcggcctct
      241 ctgaggacac cctggtgcta cccccggcca gccgcaacca gaggattctc tacaccgtgc
      301 tggagtgcca gcccctcttc gactccagtg acatgaccat cgctgagtgg gtttgccttg
      361 cccagaccat caagaggcac tacgagcagt accacggctt tgtggtcatc cacggcaccg
      421 acaccatggc ctttgctgcc tcgatgctgt ccttcatgct ggagaacctg cagaagactg
      481 tcatcctcac tggggcccag gtgcccatcc atgccctgtg gagcgacggc cgtgagaacc
      541 tgctgggggc actgctcatg gctggccagt atgtgatccc agaggtctgc cttttcttcc
      601 agaatcagct gtttcggggc aaccgggcaa ccaaggtaga cgctcggagg ttcgcagctt
      661 tctgctcccc gaacctgctg cctctggcca cagtgggtgc tgacatcaca atcaacaggg
      721 agctggtgcg gaaggtggac gggaaggctg ggctggtggt gcacagcagc atggagcagg
      781 acgtgggcct gctgcgcctc taccctggga tccctgccgc cctggttcgg gccttcttgc
      841 agcctcccct gaagggcgtg gtcatggaga ccttcggttc agggaacgga cccaccaagc
      901 ccgacctgct gcaggagctg cgggtggcca ccgagcgcgg cctggtcatc gtcaactgta
      961 cccactgcct ccagggggct gtgaccacag actatgcagc tggcatggcc atggcgggag
     1021 ccggcgtcat ctcaggcttc gacatgacat cggaggccgc cctggccaag ctatcgtatg
     1081 tgctgggcca gccagggctg agcctggatg tcaggaagga gctgctgacc aaggaccttc
     1141 ggggggagat gacgccaccc tcggtggaag agcgccggcc ctcactgcag ggcaacacgc
     1201 tgggcggtgg ggtctcctgg ctcctcagtc tgagcggcag ccaggaggca gatgccctgc
     1261 ggaatgccct ggtgcccagc ctggcctgtg ctgctgccca cgccggtgac gtggaggcgc
     1321 tgcaggcgct tgtggagctg ggcagtgacc tgggcctggt ggactttaac ggccaaaccc
     1381 cactgcacgc ggccgcccgg ggaggccaca cagaggcagt caccatgctg ctgcagagag
     1441 gtgtggacgt gaacacccgg gacacggatg gcttcagccc gctgctgctg gccgtgcggg
     1501 gcaggcatcc gggtgtcatt gggttgctgc gggaagccgg ggcctccctg tccacccagg
     1561 agctggagga agcagggacg gagctgtgca ggctggcata cagggccgac ctcgaaggcc
     1621 tgcaggtgtg gtggcaggca ggggctgacc tggggcagcc gggctatgac gggcacagcg
     1681 ccctgcacgt cgcagaggca gccgggaacc tggcagtggt ggcctttcta cagagcctgg
     1741 agggtgcggt tggtgcccag gccccatgcc cagaagtgct gcctggtgtc taacctgaag
     1801 gcgtcctgct gcagtataag ccattccttc ctcccatgac ctgctggagg ggtctcaggc
     1861 atgaccccac tgctggggct gcttcccagc ctgctctcat gtaaagcctg aaggcctttg
     1921 ttgggcagga cggcaataaa gtctctgaca tcccctcacc aggtctgtac agcctggctc
     1981 tgagaggctc tgtctgggtc cgggactgtg gatgtgtgtg gggagtcagg cccaggctct
     2041 gtggggtctc tgcgggggtc acttggccca tccttccggg ggcagctgtg cgtgtgagct
     2101 gggcagggtg gggtgcatgg acgtgacttg gccaagtggt cctttcccag ctgccactcc
     2161 cccttcctgc caccctgcct ttgcccaggc tggtccctcc tccagacacc ctctgcccat
     2221 ctccttacct cctgcgcctt cattctggag cttgaagtcc cctcccccag gcagccctcc
     2281 aacccctgga tgagcatgct gcccctgctt aactctttag tcctcacagt cccgtcccag
     2341 ttgctgtctg gttttctgtc accccagtat cgctgcaccc ggcccccctc tcaggccagg
     2401 gccacaccag ggctccatag ggctgcggaa gcctgtgagt gtcaaggggg cttcacactc
     2461 tggtggtgag cccctgggga ccattccagc ccctgctctg cctgaagctc ctgatgcagc
     2521 ctccaccgtg agccaggcca ccctgtaagg gaagaggtga gctgtgcctg cctggttgca
     2581 cagcctgggc aggtgggcgg gtggggcggg ccctgctgct gcccggtggg ggtggtgctc
     2641 caaggccaag ggcagcctcg tgccttcttt cagctggggc tgaaatcggg ggtgtggacc
     2701 cctggccccc atcatgtgtg catgcactgg cctgggatgg ccagggactc cctgctgctg
     2761 cccccagccc ctgacccctt cttgctgggt gtgaggagca tgggggctgc ctccctccag
     2821 gcctctctac ctgcccaggt gacggttcct tttgtccctc tgtttggctg gcctctggac
     2881 tggagcccca ggagggcaaa agcatgaccc cagagctggc ccttggcacc tgggcatacc
     2941 ccaggctcct cagcctggtt cccaagcctg gctggtactg ccgctggcat ccttgctgtc
     3001 ctcaccccga cacaggtgtg cctgggcctt gcacaacaag gtgacgccat gcgagcgtgg
     3061 cagggccggg gaagcctttg ctcctcaccc tggtgaggcc catgaggggc tgcaggtgga
     3121 gggagggtgg caccgggccc cagagccagg agcccctgct gtccccggat ggccgagacc
     3181 cttggagggc cttgggagag gcggtggggc atctggacaa atgcctggtg caggtgagaa
     3241 ggggtgggac tacctccggc atccctgggc tgagcccacc tggtctcccc ggttgctcct
     3301 gcacacggca gtgcccacct gcttccctca gctcctgagg ggctgggcag agcctgttgc
     3361 tcacgccacc ccactcagtg ctgctccggg ttgtgaaagc tgcaggtggc tcacgttagc
     3421 ccacatggca gcaccctccc agagcaggtg ccaacctgcc agtgtagaca caccgactga
     3481 tgccgggggt gagtgtcctg tagtgtagaa acgcccactc atcctgcttg tcccgcagag
     3541 agcatttgtc aagctggcct ttctccaact gggtcactga tggagttcgg cagagacggc
     3601 ttgggggcca cgggctgggc agatggggag agctcctcac aaggctgggc agccagaccc
     3661 ccattgtcag tcccctccaa accctcgtgg gatgtagggc cctaccacag ggagccccgg
     3721 gactgggcca gtgtctcagg aggctgtgaa gagtcatagg cttgagtctg ggagctttct
     3781 cgggggccgt tttgtgcccc ttggggagag gccagagggg ccctgggtaa ggggactcct
     3841 ctagaatcac ccagaagtgg caggaggtgc agggccactg ctcggggctc cctgggctct
     3901 gagctgggca gtgacctggc cccaaggcct ctggctctct ggcgtgcagt gggaacagcc
     3961 gggacagaca ggcttggagc gttcgtgtcc tgcgccgcgt tggtgtgttt cgtccccact
     4021 gtgcctgtag gagggcaccg ggtccccgtc tatcctactt catggctgag gaagctaagg
     4081 cacagagtgg ccccgtcctg cgcccagggc tctgcgttgg cacagggcag agctgggctg
     4141 tgaatcaggg ccgccttgtc tcttgctgtg gtgtgaggct gtgggagact caggaatgag
     4201 gcacagccag gtgcccagtg cccgtgggac ccgcctcccc acagtggggt ggcgccgctc
     4261 acacctggta atttcacgct gggggaggcc ttggttccta tagcccatct cgaatcacct
     4321 ggatcccagg gctatctctg gggaacatct cctctccatc acgactactg tctttttcac
     4381 cagcattttc tatcctaaaa tcagactcct cagctgaggg ccctccagga ccgcccttcc
     4441 ccagctggag gtaaccagat ttctcgtgta tgctaggaag ttccccttct ggtcacacct
     4501 caatatttcc tgctgctggt gggaactgca ggtgcttgta cttgtcacct tctccccgtc
     4561 ctctctcgtc agttcccttg gatgctggac acgggagcca cctgggcgag gggagcccat
     4621 ccaagtggct gtgcgtcggg aagagtctgc ccaggactgg gctagaccag aacttagaat
     4681 taaggagtgg tggccaggct tggtggctca cacctgcaat cccagcactt tgggaggccg
     4741 aggcgggtgg atagcttgag cctaggagtt tgagaacagc caacatggcg aaactctgtc
     4801 tcttaaaaaa taaataaaaa cattagccag g
//
