LOCUS       NM_183356               2663 bp    mRNA    linear   PRI 13-OCT-2024
DEFINITION  Homo sapiens asparagine synthetase (glutamine-hydrolyzing) (ASNS),
            transcript variant 3, mRNA.
ACCESSION   NM_183356
VERSION     NM_183356.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Jung,A.R., Shin,S., Kim,M.Y., Ha,U.S., Hong,S.H., Lee,J.Y.,
            Kim,S.W., Chung,Y.J. and Park,Y.H.
  TITLE     Integrated Bioinformatics Analysis Identified ASNS and DDIT3 as the
            Therapeutic Target in Castrate-Resistant Prostate Cancer
  JOURNAL   Int J Mol Sci 25 (5), 2836 (2024)
   PUBMED   38474084
  REMARK    GeneRIF: Integrated Bioinformatics Analysis Identified ASNS and
            DDIT3 as the Therapeutic Target in Castrate-Resistant Prostate
            Cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Wu,Z., Wu,Z., Zeng,J., Liu,Y., Wang,Y., Li,H., Xia,T., Liu,W.,
            Lin,Z. and Xu,W.
  TITLE     An endoplasmic reticulum stress-related signature featuring ASNS
            for predicting prognosis and immune landscape in prostate cancer
  JOURNAL   Aging (Albany NY) 16 (1), 43-65 (2024)
   PUBMED   38206293
  REMARK    GeneRIF: An endoplasmic reticulum stress-related signature
            featuring ASNS for predicting prognosis and immune landscape in
            prostate cancer.
REFERENCE   3  (bases 1 to 2663)
  AUTHORS   Cai,D.J., Zhang,Z.Y., Bu,Y., Li,L., Deng,Y.Z., Sun,L.Q., Hu,C.P.
            and Li,M.
  TITLE     Asparagine synthetase regulates lung-cancer metastasis by
            stabilizing the beta-catenin complex and modulating mitochondrial
            response
  JOURNAL   Cell Death Dis 13 (6), 566 (2022)
   PUBMED   35739087
  REMARK    GeneRIF: Asparagine synthetase regulates lung-cancer metastasis by
            stabilizing the beta-catenin complex and modulating mitochondrial
            response.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2663)
  AUTHORS   Liu,L., Wang,J., Li,H., Dong,Y., Li,Y., Xia,L., Yang,B., Wang,H.,
            Xu,Y., Cheng,G., Du,K., Zhang,X., Zhu,C., Cui,S. and Ren,C.
  TITLE     An intractable epilepsy phenotype of ASNS novel mutation in two
            patients with asparagine synthetase deficiency
  JOURNAL   Clin Chim Acta 531, 331-336 (2022)
   PUBMED   35469797
  REMARK    GeneRIF: An intractable epilepsy phenotype of ASNS novel mutation
            in two patients with asparagine synthetase deficiency.
REFERENCE   5  (bases 1 to 2663)
  AUTHORS   Heng,H.H., Shi,X.M., Scherer,S.W., Andrulis,I.L. and Tsui,L.C.
  TITLE     Refined localization of the asparagine synthetase gene (ASNS) to
            chromosome 7, region q21.3, and characterization of the somatic
            cell hybrid line 4AF/106/KO15
  JOURNAL   Cytogenet Cell Genet 66 (2), 135-138 (1994)
   PUBMED   7904551
REFERENCE   6  (bases 1 to 2663)
  AUTHORS   Alfadhel,M. and El-Hattab,A.W.
  TITLE     Asparagine Synthetase Deficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   30234940
REFERENCE   7  (bases 1 to 2663)
  AUTHORS   Van Heeke,G. and Schuster,S.M.
  TITLE     The N-terminal cysteine of human asparagine synthetase is essential
            for glutamine-dependent activity
  JOURNAL   J Biol Chem 264 (33), 19475-19477 (1989)
   PUBMED   2573597
REFERENCE   8  (bases 1 to 2663)
  AUTHORS   Greco,A., Gong,S.S., Ittmann,M. and Basilico,C.
  TITLE     Organization and expression of the cell cycle gene, ts11, that
            encodes asparagine synthetase
  JOURNAL   Mol Cell Biol 9 (6), 2350-2359 (1989)
   PUBMED   2569668
REFERENCE   9  (bases 1 to 2663)
  AUTHORS   Van Heeke,G. and Schuster,S.M.
  TITLE     Expression of human asparagine synthetase in Escherichia coli
  JOURNAL   J Biol Chem 264 (10), 5503-5509 (1989)
   PUBMED   2564390
REFERENCE   10 (bases 1 to 2663)
  AUTHORS   Zhang,Y.P., Lambert,M.A., Cairney,A.E., Wills,D., Ray,P.N. and
            Andrulis,I.L.
  TITLE     Molecular structure of the human asparagine synthetase gene
  JOURNAL   Genomics 4 (3), 259-265 (1989)
   PUBMED   2565875
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA160359.1, BC014621.2,
            AA948141.1 and AC005326.2.
            
            On Aug 14, 2020 this sequence version replaced NM_183356.3.
            
            Summary: The protein encoded by this gene is involved in the
            synthesis of asparagine. This gene complements a mutation in the
            temperature-sensitive hamster mutant ts11, which blocks progression
            through the G1 phase of the cell cycle at nonpermissive
            temperature. Alternatively spliced transcript variants have been
            described for this gene. [provided by RefSeq, May 2010].
            
            Transcript Variant: This variant (3) differs in the 5' UTR compared
            to variant 1. Variants 1, 2, 3, and 7 encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC008723.2, SRR11853566.25519.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-493               DA160359.1         46-538
            494-2212            BC014621.2         226-1944
            2213-2223           AA948141.1         6-16                c
            2224-2663           AC005326.2         78234-78673         c
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q21.3"
     gene            1..2663
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /note="asparagine synthetase (glutamine-hydrolyzing)"
                     /db_xref="GeneID:440"
                     /db_xref="HGNC:HGNC:753"
                     /db_xref="MIM:108370"
     exon            1..58
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            59..336
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            337..372
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            373..644
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     CDS             396..2081
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /EC_number="6.3.5.4"
                     /note="isoform a is encoded by transcript variant 3;
                     glutamine-dependent asparagine synthetase; TS11 cell cycle
                     control protein"
                     /codon_start=1
                     /product="asparagine synthetase [glutamine-hydrolyzing]
                     isoform a"
                     /protein_id="NP_899199.2"
                     /db_xref="CCDS:CCDS5652.1"
                     /db_xref="GeneID:440"
                     /db_xref="HGNC:HGNC:753"
                     /db_xref="MIM:108370"
                     /translation="MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCC
                     FGFHRLAVVDPLFGMQPIRVKKYPYLWLCYNGEIYNHKKMQQHFEFEYQTKVDGEIIL
                     HLYDKGGIEQTICMLDGVFAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSE
                     AKGLVTLKHSATPFLKVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDVPLHALYDN
                     VEKLFPGFEIETVKNNLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKE
                     AQVQYPLQTFAIGMEDSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETY
                     DITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESER
                     LLRELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPPEMRIPKNGIEKHLLRE
                     TFEDSNLIPKEILWRPKEAFSDGITSVKNSWFKILQEYVEHQVDDAMMANAAQKFPFN
                     TPKTKEGYYYRQVFERHYPGRADWLSHYWMPKWINATDPSARTLTHYKSAVKA"
     exon            645..882
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1068
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1170
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1298
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1425
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1532
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..1633
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1715
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1871
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1872..2663
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2192..2197
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /note="hexamer: AATAAA"
     polyA_site      2212
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /note="major polyA site"
     regulatory      2641..2646
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /note="hexamer: AATAAA"
     polyA_site      2663
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
ORIGIN      
        1 aagctgtcca catccctggc ctcagcccgc cacatcaccc tgacctgctt acgcccagcc
       61 tgtggacatt ttcgcggtcc gggtccctgc ggcggacggg tcggatcgtg ctcgtggcca
      121 gggcgcagtc aggtgtcgcg tggtcctgat ctaggaagag actgcggcct tatcaccgtc
      181 tcggtgtctg ctttcacctt caactgcccc cctcctcacc tggaccgctc accctcctcc
      241 tcccctctca ccccgccatc cccaccagtc cccacctgtc tctgtagact gcaagactca
      301 tcaagtgact tgctgagcaa accctgccga gaagagattt tcttcaatca catctgaata
      361 aatcacttga agaaagctta tagcttcatt gcaccatgtg tggcatttgg gcgctgtttg
      421 gcagtgatga ttgcctttct gttcagtgtc tgagtgctat gaagattgca cacagaggtc
      481 cagatgcatt ccgttttgag aatgtcaatg gatacaccaa ctgctgcttt ggatttcacc
      541 ggttggcggt agttgacccg ctgtttggaa tgcagccaat tcgagtgaag aaatatccgt
      601 atttgtggct ctgttacaat ggtgaaatct acaaccataa gaagatgcaa cagcattttg
      661 aatttgaata ccagaccaaa gtggatggtg agataatcct tcatctttat gacaaaggag
      721 gaattgagca aacaatttgt atgttggatg gtgtgtttgc atttgtttta ctggatactg
      781 ccaataagaa agtgttcctg ggtagagata catatggagt cagacctttg tttaaagcaa
      841 tgacagaaga tggatttttg gctgtatgtt cagaagctaa aggtcttgtt acattgaagc
      901 actccgcgac tcccttttta aaagtggagc cttttcttcc tggacactat gaagttttgg
      961 atttaaagcc aaatggcaaa gttgcatccg tggaaatggt taaatatcat cactgtcggg
     1021 atgtacccct gcacgccctc tatgacaatg tggagaaact ctttccaggt tttgagatag
     1081 aaactgtgaa gaacaacctc aggatccttt ttaataatgc tgtaaagaaa cgtttgatga
     1141 cagacagaag gattggctgc cttttatcag ggggcttgga ctccagcttg gttgctgcca
     1201 ctctgttgaa gcagctgaaa gaagcccaag tacagtatcc tctccagaca tttgcaattg
     1261 gcatggaaga cagccccgat ttactggctg ctagaaaggt ggcagatcat attggaagtg
     1321 aacattatga agtccttttt aactctgagg aaggcattca ggctctggat gaagtcatat
     1381 tttccttgga aacttatgac attacaacag ttcgtgcttc agtaggtatg tatttaattt
     1441 ccaagtatat tcggaagaac acagatagcg tggtgatctt ctctggagaa ggatcagatg
     1501 aacttacgca gggttacata tattttcaca aggctccttc tcctgaaaaa gccgaggagg
     1561 agagtgagag gcttctgagg gaactctatt tgtttgatgt tctccgcgca gatcgaacta
     1621 ctgctgccca tggtcttgaa ctgagagtcc catttctaga tcatcgattt tcttcctatt
     1681 acttgtctct gccaccagaa atgagaattc caaagaatgg gatagaaaaa catctcctga
     1741 gagagacgtt tgaggattcc aatctgatac ccaaagagat tctctggcga ccaaaagaag
     1801 ccttcagtga tggaataact tcagttaaga attcctggtt taagatttta caggaatacg
     1861 ttgaacatca ggttgatgat gcaatgatgg caaatgcagc ccagaaattt cccttcaata
     1921 ctcctaaaac caaagaagga tattactacc gtcaagtctt tgaacgccat tacccaggcc
     1981 gggctgactg gctgagccat tactggatgc ccaagtggat caatgccact gacccttctg
     2041 cccgcacgct gacccactac aagtcagctg tcaaagctta ggtggtcttt atgctgtaat
     2101 gtgaaagcaa atatttcttc gtgttggatg gggactgtgg gtagataggg gaacaatgag
     2161 agtcaactca ggctaacttg ggtgtgaaaa aaataaaagt cctaaatcta aaccattgct
     2221 gtagtcattt atgttcatgc agtctcttca taagaggtaa ctagaactgt tttgctgtat
     2281 cacagtaaag tggagggtaa gctaggaaaa gggaaaatga tcagatgtct gcaagaactc
     2341 acagccctct cctggcagag ctgctttaga tgacatttgc atcttgtgcc cgtgtggctt
     2401 ccttccagtc cttgcctcat cttagggacg tcatcaaagg actacactat ttttgttctg
     2461 cctacattcc taacagctgc ctaggcagac tcagacttga atcttctata ggagttcatc
     2521 acagctctat tttgcaagat agtagttcat ttttttctct tttatgttga gccaaagcca
     2581 cttctggaga cttgtcctag agcaggggtc aggctttttt tgaattttta atttgttttt
     2641 aataaagagc cagatagtaa ata
//
