LOCUS       NM_133436               2339 bp    mRNA    linear   PRI 06-OCT-2024
DEFINITION  Homo sapiens asparagine synthetase (glutamine-hydrolyzing) (ASNS),
            transcript variant 1, mRNA.
ACCESSION   NM_133436
VERSION     NM_133436.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Jung,A.R., Shin,S., Kim,M.Y., Ha,U.S., Hong,S.H., Lee,J.Y.,
            Kim,S.W., Chung,Y.J. and Park,Y.H.
  TITLE     Integrated Bioinformatics Analysis Identified ASNS and DDIT3 as the
            Therapeutic Target in Castrate-Resistant Prostate Cancer
  JOURNAL   Int J Mol Sci 25 (5), 2836 (2024)
   PUBMED   38474084
  REMARK    GeneRIF: Integrated Bioinformatics Analysis Identified ASNS and
            DDIT3 as the Therapeutic Target in Castrate-Resistant Prostate
            Cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Wu,Z., Wu,Z., Zeng,J., Liu,Y., Wang,Y., Li,H., Xia,T., Liu,W.,
            Lin,Z. and Xu,W.
  TITLE     An endoplasmic reticulum stress-related signature featuring ASNS
            for predicting prognosis and immune landscape in prostate cancer
  JOURNAL   Aging (Albany NY) 16 (1), 43-65 (2024)
   PUBMED   38206293
  REMARK    GeneRIF: An endoplasmic reticulum stress-related signature
            featuring ASNS for predicting prognosis and immune landscape in
            prostate cancer.
REFERENCE   3  (bases 1 to 2339)
  AUTHORS   Cai,D.J., Zhang,Z.Y., Bu,Y., Li,L., Deng,Y.Z., Sun,L.Q., Hu,C.P.
            and Li,M.
  TITLE     Asparagine synthetase regulates lung-cancer metastasis by
            stabilizing the beta-catenin complex and modulating mitochondrial
            response
  JOURNAL   Cell Death Dis 13 (6), 566 (2022)
   PUBMED   35739087
  REMARK    GeneRIF: Asparagine synthetase regulates lung-cancer metastasis by
            stabilizing the beta-catenin complex and modulating mitochondrial
            response.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2339)
  AUTHORS   Liu,L., Wang,J., Li,H., Dong,Y., Li,Y., Xia,L., Yang,B., Wang,H.,
            Xu,Y., Cheng,G., Du,K., Zhang,X., Zhu,C., Cui,S. and Ren,C.
  TITLE     An intractable epilepsy phenotype of ASNS novel mutation in two
            patients with asparagine synthetase deficiency
  JOURNAL   Clin Chim Acta 531, 331-336 (2022)
   PUBMED   35469797
  REMARK    GeneRIF: An intractable epilepsy phenotype of ASNS novel mutation
            in two patients with asparagine synthetase deficiency.
REFERENCE   5  (bases 1 to 2339)
  AUTHORS   Heng,H.H., Shi,X.M., Scherer,S.W., Andrulis,I.L. and Tsui,L.C.
  TITLE     Refined localization of the asparagine synthetase gene (ASNS) to
            chromosome 7, region q21.3, and characterization of the somatic
            cell hybrid line 4AF/106/KO15
  JOURNAL   Cytogenet Cell Genet 66 (2), 135-138 (1994)
   PUBMED   7904551
REFERENCE   6  (bases 1 to 2339)
  AUTHORS   Alfadhel,M. and El-Hattab,A.W.
  TITLE     Asparagine Synthetase Deficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   30234940
REFERENCE   7  (bases 1 to 2339)
  AUTHORS   Van Heeke,G. and Schuster,S.M.
  TITLE     The N-terminal cysteine of human asparagine synthetase is essential
            for glutamine-dependent activity
  JOURNAL   J Biol Chem 264 (33), 19475-19477 (1989)
   PUBMED   2573597
REFERENCE   8  (bases 1 to 2339)
  AUTHORS   Greco,A., Gong,S.S., Ittmann,M. and Basilico,C.
  TITLE     Organization and expression of the cell cycle gene, ts11, that
            encodes asparagine synthetase
  JOURNAL   Mol Cell Biol 9 (6), 2350-2359 (1989)
   PUBMED   2569668
REFERENCE   9  (bases 1 to 2339)
  AUTHORS   Van Heeke,G. and Schuster,S.M.
  TITLE     Expression of human asparagine synthetase in Escherichia coli
  JOURNAL   J Biol Chem 264 (10), 5503-5509 (1989)
   PUBMED   2564390
REFERENCE   10 (bases 1 to 2339)
  AUTHORS   Zhang,Y.P., Lambert,M.A., Cairney,A.E., Wills,D., Ray,P.N. and
            Andrulis,I.L.
  TITLE     Molecular structure of the human asparagine synthetase gene
  JOURNAL   Genomics 4 (3), 259-265 (1989)
   PUBMED   2565875
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA961373.1, BC014621.2 and
            AA948141.1.
            
            On May 13, 2010 this sequence version replaced NM_133436.2.
            
            Summary: The protein encoded by this gene is involved in the
            synthesis of asparagine. This gene complements a mutation in the
            temperature-sensitive hamster mutant ts11, which blocks progression
            through the G1 phase of the cell cycle at nonpermissive
            temperature. Alternatively spliced transcript variants have been
            described for this gene. [provided by RefSeq, May 2010].
            
            Transcript Variant: This variant (1) is the longest variant.
            Variants 1, 2, 3, and 7 encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK000379.1, SRR14038193.2960723.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-144               DA961373.1         1-144
            145-447             DA961373.1         147-449
            448-2323            BC014621.2         69-1944
            2324-2339           AA948141.1         1-16                c
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q21.3"
     gene            1..2339
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /note="asparagine synthetase (glutamine-hydrolyzing)"
                     /db_xref="GeneID:440"
                     /db_xref="HGNC:HGNC:753"
                     /db_xref="MIM:108370"
     exon            1..447
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            448..483
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            484..755
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     CDS             507..2192
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /EC_number="6.3.5.4"
                     /note="isoform a is encoded by transcript variant 1;
                     glutamine-dependent asparagine synthetase; TS11 cell cycle
                     control protein"
                     /codon_start=1
                     /product="asparagine synthetase [glutamine-hydrolyzing]
                     isoform a"
                     /protein_id="NP_597680.2"
                     /db_xref="CCDS:CCDS5652.1"
                     /db_xref="GeneID:440"
                     /db_xref="HGNC:HGNC:753"
                     /db_xref="MIM:108370"
                     /translation="MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCC
                     FGFHRLAVVDPLFGMQPIRVKKYPYLWLCYNGEIYNHKKMQQHFEFEYQTKVDGEIIL
                     HLYDKGGIEQTICMLDGVFAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSE
                     AKGLVTLKHSATPFLKVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDVPLHALYDN
                     VEKLFPGFEIETVKNNLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKE
                     AQVQYPLQTFAIGMEDSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETY
                     DITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESER
                     LLRELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPPEMRIPKNGIEKHLLRE
                     TFEDSNLIPKEILWRPKEAFSDGITSVKNSWFKILQEYVEHQVDDAMMANAAQKFPFN
                     TPKTKEGYYYRQVFERHYPGRADWLSHYWMPKWINATDPSARTLTHYKSAVKA"
     exon            756..993
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1179
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1281
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..1409
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1536
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1643
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1644..1744
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1745..1826
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1827..1982
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2334
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2303..2308
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /note="hexamer: AATAAA"
     polyA_site      2323
                     /gene="ASNS"
                     /gene_synonym="ASNSD; TS11"
                     /note="major polyA site"
ORIGIN      
        1 gattgttacg cacctgacag ctagcggaag gacctttctg tcctggttcc ctgaagcatc
       61 gagagggaac cgcggccggc aggccggacc gctccggaga ggaggcagcg gcagtttgag
      121 cccctgtaaa tgttatatgt gtactttttt ttactatgtg gcgtcgaagc ctgtggacat
      181 tttcgcggtc cgggtccctg cggcggacgg gtcggatcgt gctcgtggcc agggcgcagt
      241 caggtgtcgc gtggtcctga tctaggaaga gactgcggcc ttatcaccgt ctcggtgtct
      301 gctttcacct tcaactgccc ccctcctcac ctggaccgct caccctcctc ctcccctctc
      361 accccgccat ccccaccagt ccccacctgt ctctgtagac tgcaagactc atcaagtgac
      421 ttgctgagca aaccctgccg agaagagatt ttcttcaatc acatctgaat aaatcacttg
      481 aagaaagctt atagcttcat tgcaccatgt gtggcatttg ggcgctgttt ggcagtgatg
      541 attgcctttc tgttcagtgt ctgagtgcta tgaagattgc acacagaggt ccagatgcat
      601 tccgttttga gaatgtcaat ggatacacca actgctgctt tggatttcac cggttggcgg
      661 tagttgaccc gctgtttgga atgcagccaa ttcgagtgaa gaaatatccg tatttgtggc
      721 tctgttacaa tggtgaaatc tacaaccata agaagatgca acagcatttt gaatttgaat
      781 accagaccaa agtggatggt gagataatcc ttcatcttta tgacaaagga ggaattgagc
      841 aaacaatttg tatgttggat ggtgtgtttg catttgtttt actggatact gccaataaga
      901 aagtgttcct gggtagagat acatatggag tcagaccttt gtttaaagca atgacagaag
      961 atggattttt ggctgtatgt tcagaagcta aaggtcttgt tacattgaag cactccgcga
     1021 ctcccttttt aaaagtggag ccttttcttc ctggacacta tgaagttttg gatttaaagc
     1081 caaatggcaa agttgcatcc gtggaaatgg ttaaatatca tcactgtcgg gatgtacccc
     1141 tgcacgccct ctatgacaat gtggagaaac tctttccagg ttttgagata gaaactgtga
     1201 agaacaacct caggatcctt tttaataatg ctgtaaagaa acgtttgatg acagacagaa
     1261 ggattggctg ccttttatca gggggcttgg actccagctt ggttgctgcc actctgttga
     1321 agcagctgaa agaagcccaa gtacagtatc ctctccagac atttgcaatt ggcatggaag
     1381 acagccccga tttactggct gctagaaagg tggcagatca tattggaagt gaacattatg
     1441 aagtcctttt taactctgag gaaggcattc aggctctgga tgaagtcata ttttccttgg
     1501 aaacttatga cattacaaca gttcgtgctt cagtaggtat gtatttaatt tccaagtata
     1561 ttcggaagaa cacagatagc gtggtgatct tctctggaga aggatcagat gaacttacgc
     1621 agggttacat atattttcac aaggctcctt ctcctgaaaa agccgaggag gagagtgaga
     1681 ggcttctgag ggaactctat ttgtttgatg ttctccgcgc agatcgaact actgctgccc
     1741 atggtcttga actgagagtc ccatttctag atcatcgatt ttcttcctat tacttgtctc
     1801 tgccaccaga aatgagaatt ccaaagaatg ggatagaaaa acatctcctg agagagacgt
     1861 ttgaggattc caatctgata cccaaagaga ttctctggcg accaaaagaa gccttcagtg
     1921 atggaataac ttcagttaag aattcctggt ttaagatttt acaggaatac gttgaacatc
     1981 aggttgatga tgcaatgatg gcaaatgcag cccagaaatt tcccttcaat actcctaaaa
     2041 ccaaagaagg atattactac cgtcaagtct ttgaacgcca ttacccaggc cgggctgact
     2101 ggctgagcca ttactggatg cccaagtgga tcaatgccac tgacccttct gcccgcacgc
     2161 tgacccacta caagtcagct gtcaaagctt aggtggtctt tatgctgtaa tgtgaaagca
     2221 aatatttctt cgtgttggat ggggactgtg ggtagatagg ggaacaatga gagtcaactc
     2281 aggctaactt gggtgtgaaa aaaataaaag tcctaaatct aaaccattgc tgtaaaaaa
//
