LOCUS       NM_001261832            2956 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ASH2 like, histone lysine methyltransferase complex
            subunit (ASH2L), transcript variant 3, mRNA.
ACCESSION   NM_001261832
VERSION     NM_001261832.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Batbayar,G., Ishimura,A., Lyu,H., Wanna-Udom,S., Meguro-Horike,M.,
            Terashima,M., Horike,S.I., Takino,T. and Suzuki,T.
  TITLE     ASH2L, a COMPASS core subunit, is involved in the cell invasion and
            migration of triple-negative breast cancer cells through the
            epigenetic control of histone H3 lysine 4 methylation
  JOURNAL   Biochem Biophys Res Commun 669, 19-29 (2023)
   PUBMED   37262949
  REMARK    GeneRIF: ASH2L, a COMPASS core subunit, is involved in the cell
            invasion and migration of triple-negative breast cancer cells
            through the epigenetic control of histone H3 lysine 4 methylation.
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2956)
  AUTHORS   Lee,Y.T., Ayoub,A., Park,S.H., Sha,L., Xu,J., Mao,F., Zheng,W.,
            Zhang,Y., Cho,U.S. and Dou,Y.
  TITLE     Mechanism for DPY30 and ASH2L intrinsically disordered regions to
            modulate the MLL/SET1 activity on chromatin
  JOURNAL   Nat Commun 12 (1), 2953 (2021)
   PUBMED   34012049
  REMARK    GeneRIF: Mechanism for DPY30 and ASH2L intrinsically disordered
            regions to modulate the MLL/SET1 activity on chromatin.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2956)
  AUTHORS   Li,X., Qu,M., Zhang,J., Chen,K. and Ma,X.
  TITLE     CircASH2L facilitates tumor-like biologic behaviours and
            inflammation of fibroblast-like synoviocytes via miR-129-5p/HIPK2
            axis in rheumatoid arthritis
  JOURNAL   J Orthop Surg Res 16 (1), 302 (2021)
   PUBMED   33964939
  REMARK    GeneRIF: CircASH2L facilitates tumor-like biologic behaviours and
            inflammation of fibroblast-like synoviocytes via miR-129-5p/HIPK2
            axis in rheumatoid arthritis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2956)
  AUTHORS   Velot,L., Lessard,F., Berube-Simard,F.A., Tav,C., Neveu,B.,
            Teyssier,V., Boudaoud,I., Dionne,U., Lavoie,N., Bilodeau,S.,
            Pouliot,F. and Bisson,N.
  TITLE     Proximity-dependent Mapping of the Androgen Receptor Identifies
            Kruppel-like Factor 4 as a Functional Partner
  JOURNAL   Mol Cell Proteomics 20, 100064 (2021)
   PUBMED   33640491
REFERENCE   6  (bases 1 to 2956)
  AUTHORS   Hughes,C.M., Rozenblatt-Rosen,O., Milne,T.A., Copeland,T.D.,
            Levine,S.S., Lee,J.C., Hayes,D.N., Shanmugam,K.S.,
            Bhattacharjee,A., Biondi,C.A., Kay,G.F., Hayward,N.K., Hess,J.L.
            and Meyerson,M.
  TITLE     Menin associates with a trithorax family histone methyltransferase
            complex and with the hoxc8 locus
  JOURNAL   Mol Cell 13 (4), 587-597 (2004)
   PUBMED   14992727
REFERENCE   7  (bases 1 to 2956)
  AUTHORS   Wysocka,J., Myers,M.P., Laherty,C.D., Eisenman,R.N. and Herr,W.
  TITLE     Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone
            H3-K4 methyltransferase are tethered together selectively by the
            cell-proliferation factor HCF-1
  JOURNAL   Genes Dev 17 (7), 896-911 (2003)
   PUBMED   12670868
REFERENCE   8  (bases 1 to 2956)
  AUTHORS   Goo,Y.H., Sohn,Y.C., Kim,D.H., Kim,S.W., Kang,M.J., Jung,D.J.,
            Kwak,E., Barlev,N.A., Berger,S.L., Chow,V.T., Roeder,R.G.,
            Azorsa,D.O., Meltzer,P.S., Suh,P.G., Song,E.J., Lee,K.J., Lee,Y.C.
            and Lee,J.W.
  TITLE     Activating signal cointegrator 2 belongs to a novel steady-state
            complex that contains a subset of trithorax group proteins
  JOURNAL   Mol Cell Biol 23 (1), 140-149 (2003)
   PUBMED   12482968
REFERENCE   9  (bases 1 to 2956)
  AUTHORS   Wang,J., Zhou,Y., Yin,B., Du,G., Huang,X., Li,G., Shen,Y., Yuan,J.
            and Qiang,B.
  TITLE     ASH2L: alternative splicing and downregulation during induced
            megakaryocytic differentiation of multipotential leukemia cell
            lines
  JOURNAL   J Mol Med (Berl) 79 (7), 399-405 (2001)
   PUBMED   11466562
REFERENCE   10 (bases 1 to 2956)
  AUTHORS   Ikegawa,S., Isomura,M., Koshizuka,Y. and Nakamura,Y.
  TITLE     Cloning and characterization of ASH2L and Ash2l, human and mouse
            homologs of the Drosophila ash2 gene
  JOURNAL   Cytogenet Cell Genet 84 (3-4), 167-172 (1999)
   PUBMED   10393421
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA156139.1, AF056717.1 and AC084024.17.
            
            Transcript Variant: This variant (3) differs in the 5' UTR,
            initiates translation at a downstream, in-frame start codon and
            lacks an exon in the 3' coding region, but maintains the reading
            frame, compared to variant 1. The encoded isoform (c) is shorter
            than isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.182636.1, AF056717.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-495               DA156139.1         1-495
            496-1033            AF056717.1         350-887
            1034-1034           AC084024.17        4948-4948
            1035-1412           AF056717.1         889-1266
            1413-1413           AC084024.17        14036-14036
            1414-2143           AF056717.1         1268-1997
            2144-2956           AC084024.17        24925-25737
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p11.23"
     gene            1..2956
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /note="ASH2 like, histone lysine methyltransferase complex
                     subunit"
                     /db_xref="GeneID:9070"
                     /db_xref="HGNC:HGNC:744"
                     /db_xref="MIM:604782"
     exon            1..347
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /inference="alignment:Splign:2.1.0"
     exon            348..414
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /inference="alignment:Splign:2.1.0"
     exon            415..560
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /inference="alignment:Splign:2.1.0"
     CDS             442..1947
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /note="isoform c is encoded by transcript variant 3;
                     set1/Ash2 histone methyltransferase complex subunit ASH2;
                     ASH2-like protein; ash2 (absent, small, or homeotic)-like"
                     /codon_start=1
                     /product="set1/Ash2 histone methyltransferase complex
                     subunit ASH2 isoform c"
                     /protein_id="NP_001248761.1"
                     /db_xref="CCDS:CCDS59100.1"
                     /db_xref="GeneID:9070"
                     /db_xref="HGNC:HGNC:744"
                     /db_xref="MIM:604782"
                     /translation="MDTQAGSVDEENGRQLGEVELQCGICTKWFTADTFGIDTSSCLP
                     FMTNYSFHCNVCHHSGNTYFLRKQANLKEMCLSALANLTWQSRTQDEHPKTMFSKDKD
                     IIPFIDKYWECMTTRQRPGKMTWPNNIVKTMSKERDVFLVKEHPDPGSKDPEEDYPKF
                     GLLDQDLSNIGPAYDNQKQSSAVSTSGNLNGGIAAGSSGKGRGAKRKQQDGGTTGTTK
                     KARSDPLFSAQRLPPHGYPLEHPFNKDGYRYILAEPDPHAPDPEKLELDCWAGKPIPG
                     DLYRACLYERVLLALHDRAPQLKISDDRLTVVGEKGYSMVRASHGVRKGAWYFEITVD
                     EMPPDTAARLGWSQPLGNLQAPLGYDKFSYSWRSKKGTKFHQSIGKHYSSGYGQGDVL
                     GFYINLPEDTETAKSLPDTYKDKALIKFKSYLYFEEKDFVDKAEKSLKQTPHSEVSIN
                     FGPCFKYPPKDLTYRPMSDMGWGAVVEHTLADVLYHVETEVDGRRSPPWEP"
     exon            561..649
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /inference="alignment:Splign:2.1.0"
     exon            650..744
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /inference="alignment:Splign:2.1.0"
     exon            745..840
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /inference="alignment:Splign:2.1.0"
     exon            841..936
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1012
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1106
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1324
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1492
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1686
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1779
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..1839
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /inference="alignment:Splign:2.1.0"
     exon            1840..2956
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2557..2562
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /note="hexamer: AATAAA"
     polyA_site      2579
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /note="major polyA site"
     regulatory      2914..2919
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
                     /note="hexamer: ATTAAA"
     polyA_site      2932
                     /gene="ASH2L"
                     /gene_synonym="ASH2; ASH2L1; ASH2L2; Bre2"
ORIGIN      
        1 atcccgcggc tcctggagcc ctgccgcccg ccccctgcga acccaggccc cgccgccaat
       61 ggctcgccct gcctctgcgc cgcttggccc gtcccctctc aagcatatct cggataacgc
      121 cccttccgca cctttcacgg gcggtgggag ctgaggctcc tgtcgttatc tctgatcctt
      181 gcaccctggc aggaagctgg tagctcacac tttaacggga ggccttcaca tattccagaa
      241 aagaaaccac tttgcagtgc cagactggaa gaagtaacgg tcactctgaa aacagggtgg
      301 gagagctgcc tctctttgaa cctctcccag gaccaactct aacccaggga ggcaaacttg
      361 gtcgatgtaa gcggtggctt ggagacagaa tcatctaatg gaaaagatac actagaaggt
      421 gctggggata catcagaggt gatggatact caggcgggct ccgtggatga agagaatggc
      481 cgacagttgg gtgaggtaga gctgcaatgt gggatttgta caaaatggtt cacggctgac
      541 acatttggca tagatacctc atcctgtcta cctttcatga ccaactacag ttttcattgc
      601 aacgtctgcc atcacagtgg gaatacctat ttcctccgga agcaagcaaa cttgaaggaa
      661 atgtgcctta gtgctttggc caacctgaca tggcagtccc gaacacagga tgaacatccg
      721 aagacaatgt tctccaaaga taaggatatt ataccattta ttgataaata ctgggagtgc
      781 atgacaacca gacagagacc tgggaaaatg acttggccaa ataacattgt taaaacaatg
      841 agtaaagaaa gagatgtatt cttggtaaag gaacacccag atccaggcag taaagatcca
      901 gaagaagatt accccaaatt tggacttttg gatcaggacc ttagtaacat tggtcctgct
      961 tatgacaacc aaaaacagag cagtgctgtg tctactagtg ggaatttaaa tgggggaatt
     1021 gcagcaggaa gcagcggaaa aggacgagga gccaagcgca aacagcagga tggagggacc
     1081 acagggacca ccaagaaggc ccggagtgac cctttgtttt ctgctcagcg ccttccccct
     1141 catggctacc cattggaaca cccgtttaac aaagatggct atcggtatat tctagctgag
     1201 cctgatccgc acgcccctga ccccgagaag ctggaacttg actgctgggc aggaaaacct
     1261 attcctggag acctctacag agcctgcttg tatgaacggg ttttgttagc cctacatgat
     1321 cgagctcccc agttaaagat ctcagatgac cggctgactg tggttggaga gaagggctac
     1381 tctatggtga gggcctctca tggagtacgg aaaggtgcct ggtattttga aatcactgtg
     1441 gatgagatgc caccagatac cgctgccaga ctgggttggt cccagcccct aggaaacctt
     1501 caagctcctt taggttatga taaatttagc tattcttggc ggagcaaaaa gggaaccaag
     1561 ttccaccagt ccattggcaa acactactct tctggctatg gacagggaga cgtcctggga
     1621 ttttatatta atcttcctga agacacagag acagccaagt cattgccaga cacatacaaa
     1681 gataaggctt tgataaaatt caagagttat ttgtattttg aggaaaaaga ctttgtggat
     1741 aaagcagaga agagcctgaa gcagactccc catagtgagg tttccattaa ctttggacca
     1801 tgcttcaagt atcctccgaa ggatctcact taccgcccta tgagtgacat gggctggggc
     1861 gccgtggtag agcacaccct ggctgacgtc ttgtatcacg tggagacaga agtggatggg
     1921 aggcgcagtc ccccatggga accctgacca ggtccctctt ttctgtcaag gactttctgg
     1981 gaataatact gggggttttg tttttgtttt tgaactgtct caaatgttct cccaaagatg
     2041 ctaaaaacac agcctctcct tttagcaagt taaaaggctg ggtaggactg cgggagactg
     2101 cctgcctttc accattttct ccccacttcc agtgactgct cttattttgt gtaccataag
     2161 ccaacaaccg ctgactccag gattgcataa gccccctgtg aaatcggtgc tgtactgcat
     2221 accctgccag ctgtgacttg ttatcctact atattttcta aggagtgaat aatattgtcc
     2281 gagtaactaa cttatttaaa agacatttcc ttctgtgggc attgactgta tcccacctgt
     2341 tttccaagga aatggtaacc tgtttctgag aacacctgaa atcaatggct atacattcca
     2401 aaccaatcta aacgctattt ccttttggtg tgggtttggt tttgttcatt ttgaaataca
     2461 cttttgaaca ctgagatccg taaaactact agatctctgg aagtgtaatt gtgaaagaaa
     2521 cttgcttgca gctttaacaa aatgagaaac ttcccaaata aaacttgttt tgaagtttat
     2581 gtgacacttt gcttcccttc agattgggtg cctcttggtg acagtgttca gaaatgtaag
     2641 cagcacgagg aagggagctg gcactgggag gaagagccgg gtttctgagt tgtgttttgg
     2701 ctgctttcct attgctccca ttcttgccaa tcagccaccc cctttcctgt gaaaatctgc
     2761 caccttgagg agaggaacaa gagtttaaaa gggctaatga tctccctccc ggtcttccct
     2821 tggaacatgg atgttgatat atgtgcgggt ggtttcctgt cttgcttatc ttcctttgcc
     2881 ctgagctgat ggctaaaggg cagttttcgg actattaaag actgaaatgt aagaatgagc
     2941 cttctaggct gggcgc
//
