LOCUS       NM_018489              12037 bp    mRNA    linear   PRI 28-AUG-2024
DEFINITION  Homo sapiens ASH1 like histone lysine methyltransferase (ASH1L),
            transcript variant 2, mRNA.
ACCESSION   NM_018489
VERSION     NM_018489.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Yancoskie,M.N., Khaleghi,R., Gururajan,A., Raghunathan,A.,
            Gupta,A., Diethelm,S., Maritz,C., Sturla,S.J., Krishnan,M. and
            Naegeli,H.
  TITLE     ASH1L guards cis-regulatory elements against cyclobutane pyrimidine
            dimer induction
  JOURNAL   Nucleic Acids Res 52 (14), 8254-8270 (2024)
   PUBMED   38884271
  REMARK    GeneRIF: ASH1L guards cis-regulatory elements against cyclobutane
            pyrimidine dimer induction.
REFERENCE   2  (bases 1 to 12037)
  AUTHORS   Xie,M., Zhang,L., Han,L., Huang,L., Huang,Y., Yang,M. and Zhang,N.
  TITLE     The ASH1L-AS1-ASH1L axis controls NME1-mediated activation of the
            RAS signaling in gastric cancer
  JOURNAL   Oncogene 42 (46), 3435-3445 (2023)
   PUBMED   37805663
  REMARK    GeneRIF: The ASH1L-AS1-ASH1L axis controls NME1-mediated activation
            of the RAS signaling in gastric cancer.
REFERENCE   3  (bases 1 to 12037)
  AUTHORS   Al-Harthi,S., Li,H., Winkler,A., Szczepski,K., Deng,J.,
            Grembecka,J., Cierpicki,T. and Jaremko,L.
  TITLE     MRG15 activates histone methyltransferase activity of ASH1L by
            recruiting it to the nucleosomes
  JOURNAL   Structure 31 (10), 1200-1207 (2023)
   PUBMED   37527654
  REMARK    GeneRIF: MRG15 activates histone methyltransferase activity of
            ASH1L by recruiting it to the nucleosomes.
REFERENCE   4  (bases 1 to 12037)
  AUTHORS   Rothrock,A.T., Stewart,J. 3rd, Li,F., Racila,E. and Amin,K.
  TITLE     Exploration of INSM1 and hASH1 as additional markers in lung
            cytology samples of high-grade neuroendocrine carcinoma with
            indeterminate neuroendocrine differentiation
  JOURNAL   Diagn Cytopathol 50 (5), 230-234 (2022)
   PUBMED   35147301
  REMARK    GeneRIF: Exploration of INSM1 and hASH1 as additional markers in
            lung cytology samples of high-grade neuroendocrine carcinoma with
            indeterminate neuroendocrine differentiation.
REFERENCE   5  (bases 1 to 12037)
  AUTHORS   Liu,W., Xu,L., Zhang,C., Shen,L., Dong,J., Zhang,H., Liu,S., Che,F.
            and Zheng,X.
  TITLE     ASH1L may contribute to the risk of Tourette syndrome: Combination
            of family-based analysis and case-control study
  JOURNAL   Brain Behav 12 (4), e2539 (2022)
   PUBMED   35307981
  REMARK    GeneRIF: ASH1L may contribute to the risk of Tourette syndrome:
            Combination of family-based analysis and case-control study.
REFERENCE   6  (bases 1 to 12037)
  AUTHORS   Gregory,G.D., Vakoc,C.R., Rozovskaia,T., Zheng,X., Patel,S.,
            Nakamura,T., Canaani,E. and Blobel,G.A.
  TITLE     Mammalian ASH1L is a histone methyltransferase that occupies the
            transcribed region of active genes
  JOURNAL   Mol Cell Biol 27 (24), 8466-8479 (2007)
   PUBMED   17923682
  REMARK    GeneRIF: These data suggest that ASH1L occupies most, if not all,
            active genes and methylates histone H3 in a nonredundant fashion at
            a subset of genes.
REFERENCE   7  (bases 1 to 12037)
  AUTHORS   Vasilescu,J., Zweitzig,D.R., Denis,N.J., Smith,J.C., Ethier,M.,
            Haines,D.S. and Figeys,D.
  TITLE     The proteomic reactor facilitates the analysis of affinity-purified
            proteins by mass spectrometry: application for identifying
            ubiquitinated proteins in human cells
  JOURNAL   J Proteome Res 6 (1), 298-305 (2007)
   PUBMED   17203973
REFERENCE   8  (bases 1 to 12037)
  AUTHORS   Kuryshev,V.Y., Vorobyov,E., Zink,D., Schmitz,J.,
            Rozhdestvensky,T.S., Munstermann,E., Ernst,U., Wellenreuther,R.,
            Moosmayer,P., Bechtel,S., Schupp,I., Horst,J., Korn,B., Poustka,A.
            and Wiemann,S.
  TITLE     An anthropoid-specific segmental duplication on human chromosome
            1q22
  JOURNAL   Genomics 88 (2), 143-151 (2006)
   PUBMED   16545939
REFERENCE   9  (bases 1 to 12037)
  AUTHORS   Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
            Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
  TITLE     Functional proteomics mapping of a human signaling pathway
  JOURNAL   Genome Res 14 (7), 1324-1332 (2004)
   PUBMED   15231748
REFERENCE   10 (bases 1 to 12037)
  AUTHORS   Nakamura,T., Blechman,J., Tada,S., Rozovskaia,T., Itoyama,T.,
            Bullrich,F., Mazo,A., Croce,C.M., Geiger,B. and Canaani,E.
  TITLE     huASH1 protein, a putative transcription factor encoded by a human
            homologue of the Drosophila ash1 gene, localizes to both nuclei and
            cell-cell tight junctions
  JOURNAL   Proc Natl Acad Sci U S A 97 (13), 7284-7289 (2000)
   PUBMED   10860993
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL353807.18, BQ883186.1,
            AF257305.1, AL139410.20, CN365042.1 and DA236222.1.
            
            On Nov 23, 2018 this sequence version replaced NM_018489.2.
            
            Summary: This gene encodes a member of the trithorax group of
            transcriptional activators. The protein contains four AT hooks, a
            SET domain, a PHD-finger motif, and a bromodomain. It is localized
            to many small speckles in the nucleus, and also to cell-cell tight
            junctions. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice junction compared to variant 1. The resulting isoform (2)
            has the same N- and C-termini but is shorter compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.3350615.1,
                                           SRR14038197.1341747.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000392403.8/ ENSP00000376204.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-270               AL353807.18        93008-93277         c
            271-278             BQ883186.1         1-8
            279-279             AL353807.18        92999-92999         c
            280-441             BQ883186.1         10-171
            442-6060            AF257305.1         1-5619
            6061-6061           AL139410.20        133605-133605       c
            6062-6268           AF257305.1         5621-5827
            6269-6898           CN365042.1         3-632
            6899-8272           AF257305.1         6473-7846
            8273-8840           DA236222.1         21-588
            8841-10352          AF257305.1         8415-9926
            10353-12037         AL139410.20        30029-31713         c
FEATURES             Location/Qualifiers
     source          1..12037
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q22"
     gene            1..12037
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /note="ASH1 like histone lysine methyltransferase"
                     /db_xref="GeneID:55870"
                     /db_xref="HGNC:HGNC:19088"
                     /db_xref="MIM:607999"
     exon            1..651
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            652..1170
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     CDS             751..9645
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /EC_number="2.1.1.359"
                     /EC_number="2.1.1.367"
                     /note="isoform 2 is encoded by transcript variant 2;
                     probable histone-lysine N-methyltransferase ASH1L;
                     ASH1-like protein; lysine N-methyltransferase 2H; absent
                     small and homeotic disks protein 1 homolog; ash1 (absent,
                     small, or homeotic)-like"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase ASH1L isoform
                     2"
                     /protein_id="NP_060959.2"
                     /db_xref="CCDS:CCDS1113.2"
                     /db_xref="GeneID:55870"
                     /db_xref="HGNC:HGNC:19088"
                     /db_xref="MIM:607999"
                     /translation="MDPRNTAMLGLGSDSEGFSRKSPSAISTGTLVSKREVELEKNTK
                     EEEDLRKRNRERNIEAGKDDGLTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKN
                     LENYVCRPAIKTTIKHPRKALKSGKMTDEKNEHCPSKRDPSKLYKKADDVAAIECQSE
                     EVIRLHSQGENNPLSKKLSPVHSEMADYINATPSTLLGSRDPDLKDRALLNGGTSVTE
                     KLAQLIATCPPSKSSKTKPKKLGTGTTAGLVSKDLIRKAGVGSVAGIIHKDLIKKPTI
                     STAVGLVTKDPGKKPVFNAAVGLVNKDSVKKLGTGTTAVFINKNLGKKPGTITTVGLL
                     SKDSGKKLGIGIVPGLVHKESGKKLGLGTVVGLVNKDLGKKLGSTVGLVAKDCAKKIV
                     ASSAMGLVNKDIGKKLMSCPLAGLISKDAINLKAEALLPTQEPLKASCSTNINNQESQ
                     ELSESLKDSATSKTFEKNVVRQNKESILEKFSVRKEIINLEKEMFNEGTCIQQDSFSS
                     SEKGSYETSKHEKQPPVYCTSPDFKMGGASDVSTAKSPFSAVGESNLPSPSPTVSVNP
                     LTRSPPETSSQLAPNPLLLSSTTELIEEISESVGKNQFTSESTHLNVGHRSVGHSISI
                     ECKGIDKEVNDSKTTHIDIPRISSSLGKKPSLTSESSIHTITPSVVNFTSLFSNKPFL
                     KLGAVSASDKHCQVAESLSTSLQSKPLKKRKGRKPRWTKVVARSTCRSPKGLELERSE
                     LFKNVSCSSLSNSNSEPAKFMKNIGPPSFVDHDFLKRRLPKLSKSTAPSLALLADSEK
                     PSHKSFATHKLSSSMCVSSDLLSDIYKPKRGRPKSKEMPQLEGPPKRTLKIPASKVFS
                     LQSKEEQEPPILQPEIEIPSFKQGLSVSPFPKKRGRPKRQMRSPVKMKPPVLSVAPFV
                     ATESPSKLESESDNHRSSSDFFESEDQLQDPDDLDDSHRPSVCSMSDLEMEPDKKITK
                     RNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFG
                     SKLGQQINVSKKGTIYIGKRRGRKPKTVLNGILSGSPTSLAVLEQTAQQAAGSALGQI
                     LPPLLPSSASSSEILPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHLHSRQGSMI
                     QTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIG
                     TDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRNHDYLSY
                     DKMKRQKRKRKKKYPQLRNRQDPDFIAELEELISRLSEIRITHRSHHFIPRDLLPTIF
                     RINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGF
                     YPSYGMPYSPSPLTAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNP
                     AKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRS
                     SEQPQVSMDTGSSRSVLESLKRYRFGKDAVGERYKHKEKHRCHMSCPHLSPSKSLINR
                     EEQWVHREPSESSPLALGLQTPLQIDCSESSPSLSLGGFTPNSEPASSDEHTNLFTSA
                     IGSCRVSNPNSSGRKKLTDSPGLFSAQDTSLNRLHRKESLPSNERAVQTLAGSQPTSD
                     KPSQRPSESTNCSPTRKRSSSESTSSTVNGVPSRSPRLVASGDDSVDSLLQRMVQNED
                     QEPMEKSIDAVIATASAPPSSSPGRSHSKDRTLGKPDSLLVPAVTSDSCNNSISLLSE
                     KLTSSCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTG
                     NNFVKRRPGRPRKCPLQAVVSMQAFQAAQFVNPELNRDEEGAALHLSPDTVTDVIEAV
                     VQSVNLNPEHKKGLKRKGWLLEEQTRKKQKPLPEEEEQENNKSFNEAPVEIPSPSETP
                     AKPSEPESTLQPVLSLIPREKKPPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKL
                     EYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRS
                     NVYVDVKPLSGYEATTCNCKKPDDDTRKGCVDDCLNRMIFAECSPNTCPCGEQCCNQR
                     IQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNH
                     SDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAG
                     TELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRVNGLTSSKNSQPMATHKKSGR
                     SKEKRKSKHKLKKRRGHLSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVR
                     NWEKIRQKQEEVKHTSDNIHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRT
                     RRLAAAEENIEVARAARLAQIFKEICDGIISYKDSSRQALAAPLLNLPPKKKNADYYE
                     KISDPLDLITIEKQILTGYYKTVEAFDADMLKVFRNAEKYYGRKSPVGRDVCRLRKAY
                     YNARHEASAQIDEIVGETASEADSSETSVSEKENGHEKDDDVIRCICGLYKDEGLMIQ
                     CDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRD
                     DLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFA
                     FGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGV
                     KEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKKDFSPHYVPDNY
                     KRNGGRSSWKSERSKPPLKDLGQEDDALPLIEEVLASQEQAANEIPSLEEPEREGATA
                     NVSEGEKKTEESSQEPQSTCTPEERRHNQRERLNQILLNLLEKIPGKNAIDVTYLLEE
                     GSGRKLRRRTLFIPENSFRK"
     exon            1171..5734
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            5735..5836
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            5837..6578
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            6579..6758
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            6759..6853
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            6854..6927
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            6928..6973
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            6974..7082
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            7083..7289
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            7290..7436
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            7437..7545
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            7546..7710
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            7711..7805
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            7806..7963
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            7964..8116
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            8117..8171
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            8172..8304
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            8305..8553
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            8554..8640
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            8641..8731
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            8732..8870
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            8871..9043
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            9044..9210
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            9211..9251
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            9252..9553
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     exon            9554..12037
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /inference="alignment:Splign:2.1.0"
     regulatory      12013..12018
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /note="hexamer: ATTAAA"
     polyA_site      12037
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /note="major polyA site"
ORIGIN      
        1 gaggcttggg cttgcgccgt tgaaggtacg cgtgagggcc tgcgccggcg ccggcttgac
       61 agggcctgag gggtagtgag ggactggacg agggggttcc tgtgggtgag cgcgtacggg
      121 ctggctggcg cgcggggcgg ccgaaggtgg tggttggtgg gagcagccag cgacgagccc
      181 gtagacactc gtacgcgtgc gggcgggcgt gcgcgctacg cggacggagt cgggcggagc
      241 gggggacggt gggaaagaac gcaaggagga aggagtggaa ggttgagggg ggcgctaggc
      301 gcccttcgct ccctccctct ggaggagctg ccgccgccac cgccgccact ctgctgctgc
      361 cgccgccgcc gccgccgctc ccgccgccat tttgggttcg ctttgcggag gggagacgat
      421 cccagtctcg gttgcgggac ccgcctcccc tcagtttgcc ccctttagcc ttccaccttt
      481 cccttctcct ctctcgcatt tccgccagtc agcttacccg ctggccgcct cctgacaagc
      541 gggagggatc cgccgtggac ccagggaagc ggaggagcct ggcggccacc ccctcttccc
      601 cacttccctg cactctcatc gctctcggcc tcggcctcgg cctccgacac gagaaagatg
      661 ctggtttcga gttttggaga tccttgtttt ttatggaaca cagttctgta aaattttcat
      721 aagattcctt ggcaataaca tacgcttgtg atggacccta gaaatactgc tatgttagga
      781 ttgggttctg attccgaagg tttttcaaga aagagtcctt ctgccatcag tactggcaca
      841 ttggtcagta agagagaagt agagctagaa aaaaacacaa aggaggaaga ggaccttcgc
      901 aaacggaatc gagaaagaaa catcgaagct gggaaagatg atggtttgac tgatgcacag
      961 caacagtttt cagtgaaaga aacaaacttt tcagagggaa atttaaaatt gaaaattggc
     1021 ctccaggcta agagaactaa aaaacctcca aagaacttgg agaactatgt atgtcgacct
     1081 gccataaaaa caactattaa gcacccaagg aaagcactta aaagtggaaa gatgacggat
     1141 gaaaagaatg aacactgtcc ttcaaaacga gacccttcaa agttgtacaa gaaagcagat
     1201 gatgttgcag ccattgaatg ccagtctgaa gaagtcatcc gtcttcattc acagggagaa
     1261 aacaatcctt tgtctaagaa gctgtctcca gtacactcag aaatggcaga ttatattaat
     1321 gcaacgccat ctactcttct tggtagccgg gatcctgatt taaaggacag agcattactt
     1381 aatggaggaa ctagtgtaac agaaaagttg gcacagctga ttgctacctg tcctccttcc
     1441 aagtcttcca agacaaaacc gaagaagtta ggaactggca ctacagcagg attggttagc
     1501 aaggatttga tcaggaaagc aggtgttggc tctgtagctg gaataataca taaggactta
     1561 ataaaaaagc caaccatcag cacagcagtt ggattggtaa ctaaagatcc tgggaaaaag
     1621 ccagtgttta atgcagcagt aggattggtc aataaggact ctgtgaaaaa actgggaact
     1681 ggcactacag cggtattcat taataaaaac ttaggcaaaa agccaggaac tatcactaca
     1741 gtaggactgc taagcaaaga ttcaggaaag aagctaggaa ttggtattgt tccaggttta
     1801 gtgcataaag agtctggcaa gaagttagga cttggcactg tggttggact ggttaataaa
     1861 gatttgggaa agaaattggg ttctactgtt ggcctagtgg ccaaggactg tgcaaagaag
     1921 attgtagcaa gttcagcaat gggattggtt aataaggaca ttggaaagaa actaatgagt
     1981 tgtcctttgg caggtctgat cagtaaagat gccataaacc ttaaagccga agcactgctc
     2041 cccactcagg aaccgcttaa ggcttcttgt agtacaaaca tcaataatca ggaaagtcag
     2101 gaactttctg aatccctgaa agatagtgcc accagcaaaa cttttgaaaa gaatgttgta
     2161 cggcagaata aagaaagcat attggaaaag ttctcagtac gaaaagaaat cattaatttg
     2221 gagaaagaaa tgtttaatga aggaacatgc attcagcaag acagtttctc atccagtgaa
     2281 aagggatctt atgaaacctc aaagcatgaa aagcagcctc ctgtatattg cacttctccg
     2341 gactttaaaa tgggaggtgc ttctgatgta tctaccgcta aatccccatt cagtgcagta
     2401 ggagaaagca atctcccttc cccatcacct actgtatctg ttaatccttt aaccagaagt
     2461 ccccctgaaa cttcttcaca gttggctcct aatccattac ttttaagttc tactacagaa
     2521 ctaatcgaag aaatttctga atctgttgga aagaaccagt ttacttctga aagtacccac
     2581 ttgaacgttg gtcataggtc agttggtcat agtataagta ttgaatgtaa agggattgat
     2641 aaagaggtaa atgattcaaa aactacccat atagatattc caagaataag ctcttccctt
     2701 ggaaaaaagc caagtttgac ttctgaatcc agcattcata ctattactcc ttcagttgtt
     2761 aacttcacta gtttatttag taataagcct tttttaaaac tgggtgcagt atctgcatca
     2821 gacaaacact gccaagttgc tgaaagccta agtactagtt tgcagtccaa accattaaaa
     2881 aaaagaaaag gaagaaaacc tcggtggact aaagtggtgg caagaagcac atgccggtct
     2941 ccaaaagggc tagaattaga aagatcagag ctttttaaaa acgtttcatg tagctcacta
     3001 tcaaatagta attctgagcc agccaagttt atgaaaaaca ttggaccccc ttcatttgta
     3061 gatcatgact tccttaaacg ccgattgcca aagttgagca aatccacagc tccatctctt
     3121 gctctcttag ctgatagtga aaaaccatct cataagtctt ttgctactca caaactatcc
     3181 tccagtatgt gtgtctctag tgaccttttg tctgatattt ataagcccaa aagaggaagg
     3241 cctaaatcta aggagatgcc tcaactggaa gggccaccta aaaggacttt aaaaatccct
     3301 gcttctaaag tgttttcttt acagtctaag gaagaacaag aacccccaat tttacagcca
     3361 gaaattgaaa tcccttcctt caaacaaggt ctgtctgtgt ctccttttcc aaaaaagaga
     3421 ggcaggccta agaggcaaat gaggtcacca gtcaagatga agccacctgt actgtcagtg
     3481 gctccatttg ttgccactga aagtccaagc aagctagaat ctgaaagtga caaccataga
     3541 agtagcagtg atttctttga gagcgaggat caacttcagg atccagatga cctagatgac
     3601 agtcataggc caagtgtctg tagtatgagt gaccttgaga tggaaccaga taaaaaaatt
     3661 accaagagaa acaatggaca attaatgaaa acaattatcc gcaaaataaa taaaatgaag
     3721 actttaaaga gaaagaaact gttgaatcag attctttcaa gttctgtaga atcaagtaat
     3781 aaagggaaag tgcaatccaa actccataat acggtatcaa gtcttgctgc cacatttggc
     3841 tctaaattgg gccaacagat aaatgtcagc aagaaaggaa ccatttatat aggaaagaga
     3901 agaggtcgca aaccaaaaac tgtcttaaat ggtattcttt ctggtagtcc tactagcctt
     3961 gctgttcttg agcaaacagc tcaacaggca gctgggtcag cattaggaca gattcttccc
     4021 ccattactgc cttcatctgc tagtagttct gagattcttc catcacctat ttgctctcag
     4081 tcttctggga ctagtggagg tcagagccct gtaagtagtg atgcaggttt tgttgaaccc
     4141 agttcagtgc catatttgca tttacactcc agacagggca gtatgattca gactcttgca
     4201 atgaagaagg cctcaaaggg gaggaggcgg ttatctcctc ctactttgtt gccaaattct
     4261 ccttcgcact tgagtgaact cacatctcta aaagaagcta ctccttcccc aatcagtgag
     4321 tctcatagtg atgagaccat tcccagtgat agtggaattg gaacagataa taacagcaca
     4381 tcagacaggg cagagaaatt ttgtgggcaa aaaaagagga ggcattcttt tgagcatgtt
     4441 tctctgattc cccctgaaac ctctacagtg ctaagcagtc ttaaagaaaa acataaacac
     4501 aaatgtaagc gcaggaatca tgattacctc agctatgaca agatgaaaag gcagaaacga
     4561 aaacggaaaa agaaatatcc ccagcttcga aatagacagg atccagactt tattgcagag
     4621 ctggaggaac taataagtcg cctaagtgaa attcggatca ctcatcgaag tcatcatttt
     4681 atcccccgag atcttctgcc aactatcttt cgaatcaact ttaatagttt ctatacacat
     4741 ccttctttcc ccttagaccc tttgcactac attcgaaaac ctgacttaaa aaagaaaaga
     4801 gggagacccc ctaagatgag ggaggcaatg gctgaaatgc cttttatgca cagccttagt
     4861 tttcctcttt ctagtactgg attctatcca tcttatggta tgccttactc tccttcaccc
     4921 cttacagctg ctcccatagg attaggttac tatggaaggt atcctcccac tctttatcca
     4981 cctcctccat ctccttcttt caccacgcca cttccacctc cttcctatat gcatgctggt
     5041 catttacttc tcaatcctgc caaataccat aagaaaaagc ataagctact tcgacaggag
     5101 gcctttctta caaccagcag gactcccctc ctttccatga gtacctaccc cagtgttcct
     5161 cctgagatgg cctatggttg gatggttgag cacaaacaca ggcaccgtca caaacacaga
     5221 gaacaccgtt cttctgaaca accccaggtt tctatggaca ctggctcttc ccgatctgtc
     5281 ctggaatctt tgaagcgcta tagatttgga aaggatgctg ttggagagcg atataagcat
     5341 aaggaaaagc accgttgtca catgtcctgc cctcatctct ctccttcaaa aagcttaata
     5401 aacagagagg aacagtgggt ccaccgagag ccttcagaat ctagtccatt ggccttggga
     5461 ttgcagacac ctttacagat tgactgttca gaaagttctc caagcttatc ccttggagga
     5521 ttcactccca actctgagcc agccagcagt gatgaacata caaacctttt cacaagtgca
     5581 ataggcagct gcagagtttc aaaccctaac tccagtggcc ggaagaaatt aactgacagc
     5641 cctggactct tttctgcaca ggacacttca ctaaatcggc ttcacagaaa ggagtcactg
     5701 ccttctaacg aaagggcagt acagactttg gcaggctccc agccaacctc tgataaaccc
     5761 tcccagcggc catcagagag cacaaattgt agccctaccc ggaaaaggtc ttcatctgag
     5821 agtacttctt caacagtaaa cggagttccc tctcgaagtc caagattagt tgcttctggg
     5881 gatgactctg tggatagtct gctgcagcgg atggtacaaa atgaggacca agagcccatg
     5941 gagaaaagta ttgatgctgt gattgcaact gcctctgcac caccttcttc cagtccaggc
     6001 cgtagccaca gcaaggaccg aaccctggga aaaccagaca gccttttagt gcctgcagtc
     6061 acaagtgact cttgcaataa tagcatctca ctcctatctg aaaagttgac aagcagctgt
     6121 tccccccatc atatcaagag aagtgtagtg gaagctatgc aacgccaagc tcggaaaatg
     6181 tgcaattacg acaaaatctt ggccacaaag aaaaacctag accatgtcaa taaaatctta
     6241 aaagccaaaa aacttcaaag gcaggccagg acagggaata actttgtgaa acgtaggcca
     6301 ggtcgacctc ggaaatgtcc ccttcaggct gtcgtatcaa tgcaagcatt ccaggctgct
     6361 cagtttgtca acccagaatt gaacagagac gaggaaggag cagcactgca cctcagtcct
     6421 gacacagtta cagatgtaat tgaggctgtt gttcagagtg taaatctgaa cccagaacat
     6481 aaaaaggggt tgaagagaaa aggttggcta ttggaagaac agaccagaaa aaagcagaag
     6541 ccattaccag aggaagaaga gcaagagaat aataaaagct ttaatgaagc accagttgag
     6601 attcccagtc cttctgaaac cccagctaaa ccttctgaac ctgaaagtac cttgcagcct
     6661 gtgctttctc tcatcccaag ggaaaagaag cccccacgtc ccccaaagaa gaagtatcag
     6721 aaagcagggc tgtattctga cgtttacaaa actacagacc caaagagtcg attgatccaa
     6781 ttaaagaaag agaagctgga gtatactcca ggagagcatg aatatggatt atttccagcg
     6841 cccattcatg ttggaaagta tctaagacaa aagagaattg acttccagct tccttatgat
     6901 atcctttggc agtggaaaca caatcagcta tacaaaaagc cagatgtccc actatataag
     6961 aaaattcgtt caaatgtcta cgttgatgtc aaaccccttt ctggttacga agctaccacc
     7021 tgtaactgta agaagccaga tgatgacacc aggaagggct gtgttgatga ctgcctcaat
     7081 agaatgatct ttgctgagtg ttcccccaac acttgcccat gtggcgagca atgctgtaac
     7141 cagaggatac agaggcatga atgggtgcaa tgtctagaac gatttcgagc tgaggaaaaa
     7201 ggttggggaa tcagaaccaa agagccccta aaagctgggc agttcatcat tgaataccta
     7261 ggggaggtcg tcagtgaaca ggagttcagg aacaggatga ttgagcagta tcataatcac
     7321 agtgaccact actgcctgaa cctggatagt gggatggtga ttgacagtta ccgcatggga
     7381 aatgaggccc gattcatcaa ccatagctgt gacccaaatt gtgaaatgca gaaatggtct
     7441 gttaatggag tataccggat tggactctat gctcttaaag acatgccagc tgggactgaa
     7501 ctcacttatg attataactt tcattccttc aatgtggaaa aacagcaact ttgtaagtgt
     7561 ggctttgaga aatgtcgagg aatcatcgga ggcaagagtc agcgtgtgaa tggactcacc
     7621 agcagcaaaa acagccagcc catggccaca cacaaaaaat ctggacggtc aaaagagaag
     7681 agaaagtcta agcacaagct gaagaaaagg agaggccatc tctctgagga acccagtgaa
     7741 aatatcaaca ccccaactag attgaccccc caattacaga tgaagccaat gtccaatcgt
     7801 gaaaggaact ttgtgttaaa gcatcatgta ttcttggtcc gaaactggga gaagattcgt
     7861 caaaaacagg aggaagtaaa gcacaccagt gataatattc actcagcatc attatatacc
     7921 cgttggaatg ggatctgccg agatgatggg aatatcaagt ctgatgtctt catgacccag
     7981 ttctctgccc tgcagacagc tcgatctgtt cgaacaagac ggttggcagc tgcagaggaa
     8041 aatattgaag tggctcgggc agcccgccta gcccagatct tcaaagaaat ttgtgatggt
     8101 atcatctctt ataaagattc ttcccggcaa gcactggcag ctccactttt gaaccttccc
     8161 ccaaagaaaa agaatgctga ttattatgag aagatctctg atcccctaga tcttatcacc
     8221 atagagaagc agatcctcac tggttactat aagacagtgg aagcttttga tgctgacatg
     8281 ctcaaagtct ttcggaatgc tgagaagtac tatgggcgta aatccccagt tgggagagat
     8341 gtttgtcgtc tacgaaaggc ctattacaat gcccggcatg aggcatcagc ccagattgat
     8401 gagattgtgg gagagacagc aagtgaggca gacagcagtg agacctcagt ctctgaaaag
     8461 gagaatgggc atgagaagga cgacgatgtt attcgctgta tctgtggcct ctacaaggat
     8521 gaaggtctca tgatccagtg tgacaagtgc atggtatggc agcactgtga ttgtatggga
     8581 gtgaactcag atgtggagca ctacctttgt gagcagtgtg acccaaggcc tgtggacagg
     8641 gaggttccca tgatccctcg gccccactat gcccaacctg gctgtgtcta cttcatctgt
     8701 ttgctccgag atgacttgct gcttcgtcag ggtgactgtg tgtatctgat gagggatagt
     8761 cggcgcaccc ctgatggcca cccggtccgt cagtcctatc gactgttatc tcacattaac
     8821 cgagataaac ttgacatctt tcgcattgag aagctttgga agaatgaaaa agaggaacgg
     8881 tttgcctttg gtcaccatta tttccgtccc cacgaaacac accactctcc atcccgtcgg
     8941 ttctatcata atgaactatt tcgggtgcca ctctatgaga tcattccctt ggaggctgta
     9001 gtggggacct gctgtgtgtt ggacctttat acgtattgta aagggagacc caaaggagta
     9061 aaggagcaag atgtgtacat ctgtgattat cggcttgaca agtcagcaca cctgttttac
     9121 aagatccacc ggaaccgcta tcctgtctgc accaaaccct atgcttttga tcacttcccc
     9181 aagaagctca ctcccaaaaa agatttctcg cctcattacg tcccagacaa ctacaagagg
     9241 aatggaggac gatcatcctg gaagtctgag cgctcaaagc cacccctaaa agacttgggc
     9301 caggaggatg atgctctacc cttgattgaa gaggttctag ccagtcaaga gcaagcagcc
     9361 aatgagatac ccagcctgga ggagccagaa cgggaagggg ccactgctaa cgtcagtgag
     9421 ggtgaaaaaa aaacagagga aagtagtcaa gaaccccagt caacctgtac ccctgaggaa
     9481 cgacggcata accaacggga acgactcaac cagatcttgc tcaatctcct tgaaaaaatc
     9541 cctggaaaaa atgccattga tgtgacctac ttgctggagg aaggatcagg caggaaactg
     9601 cgaaggcgta ctttgtttat cccagaaaac agctttcgaa agtgaccctc aaagaatgag
     9661 aacctcaagc atctgggatc cagtggagct aatcagtcct gcctcctgct ctctgggtat
     9721 agacaggggt gggaagggtc catctgggca aggggaatgg ggccatgttg ttgacattag
     9781 gtacttaata agccttggag ctagtggaga gggagaggaa agggttctgt ccaagacagt
     9841 tcaggttaat taattttctt ctccattgct tcaccttaag ggttaataat gtagagagga
     9901 gggaggacca cattgatgac cagaacctac tggtacttta tagcatttgc cccaccccac
     9961 agcttaggtt tttctgtcat cctcagatcc cacaggcatt gcgaagaagc tgcttcctat
    10021 acccaggtat aactcaaaat ccaaagggat agggccagga tccctattcc taccccatct
    10081 attctctgtt ggctccaaga gctaccccag agaccttaaa cagaaacagt agctgaggct
    10141 tcttcctaga tacctgacta gggaagtttg tctctccttt cttgcccaac caggtcaaag
    10201 taaaatgtga gttgacagct caaagcactt gtaactgctg ccccctccct acctctactc
    10261 cccaaaatgg aatcatggga tagggaaggc ccccatgggg tcagaagggc acggtagttc
    10321 ttgcaattat ttttgtttta cccttcataa cctgtcaaac atattttttt ctaatgagaa
    10381 agccaggccc ccgccagcac acatgctgtt tttaatgcgc tgtagttctt gtgtgtctgc
    10441 tgtgctgtgc aaatggagat tcagttcaaa ataaaatcat ttaaaaacct acataaaaag
    10501 aactctaaac ccacccctgc aacaaaagtc actacataaa ctgttcagca gtattcacct
    10561 atcagagtat ttgttgtgag tatagattat caattgaaaa cactactctt gttttcttaa
    10621 ttgtacagtt ttcaatgtcc ctttcttaaa gagacagtat atttctcttc acccctagcc
    10681 catcttccct caccctcctg aatgacatca ggaggtatat ccagggtgtc tccttccttc
    10741 ctactctctt gaccagaagt taacagacta tactgtctct ttaaaaataa aatttaaaaa
    10801 gctttgttgt cttttcagac atacatatgc atatatgttt tagatgttct tataagagaa
    10861 aagatggttt ttaaatgtgc caagttgtgt gtgtgtgtgt atatatatgt gtgtatgtgt
    10921 gtgtatatat atatgtgtgt gtgtatatat atacacacac acacacacac ctgctgtgtg
    10981 attggtaagc aatacaatag taaacatgtc cccattactt ttttctaata ttggaccaat
    11041 gctgtcctaa ttgtacattt ccccttatgg tgacgatgct ctgactcgtt taggtagaca
    11101 cattgaccac cttccattcc attaaatatt ttttcctttt tcccctttct gtgtcattct
    11161 tgaggaaaaa acaaaagaga gaggggatgc caatgatccc cttgagcaga gaaaaagcaa
    11221 aataaatatt ttattaaaga aaaaagagaa ttaagaaaat agtttggagt attttcttac
    11281 tgtagagaag cactgtacat tactaagaga cctgggtata agatactcac atgtggagct
    11341 ggaaaaatcg catgtccaag cccgtttgag tggtttcttt tgtttttcat tgcagggagt
    11401 gggtgggagg gaggtgggac taggggcact ttgggggtct ccttttagtc aaaagcgaga
    11461 aaatgacaag aaagagatta aaattcaatg tttcctttat agtgttaaac actaaaattt
    11521 taaaaaagat gaaaaagaaa aaaaaacttt gtaaaatgcg agaacagaag caaaagacac
    11581 tacgctctgt cattttatct ttcttttgtt gaaagactaa aaaaaaactg aaatgttttt
    11641 tagacaatca aatgttaggt aagtgcaaaa acttgttttt tcttactggt gtagaaatta
    11701 atgccttttt ttatttttca gttattttat aataacgaaa taaaaagaac cccccagctg
    11761 ccaggcgggt tttggtgttt gaaatgcggg gcaaagcact acatcactgc aaatagatac
    11821 agagttagtc tgcatgtctg taggctgtgt gattgcggaa aatataaatg ctgctaatat
    11881 atttcctttt tacaaaagca tatctaaata gatgattgtt ttgatgttaa tctttgtaaa
    11941 ttatgtatta ccaattttaa cattggatgt aattgcatac aaagcttgca tctcaatcct
    12001 tgaaagtcta gtattaaatg gaaaaaactt ttcctaa
//
