LOCUS       NM_005170               1485 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens achaete-scute family bHLH transcription factor 2
            (ASCL2), mRNA.
ACCESSION   NM_005170
VERSION     NM_005170.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Zheng,E., Cai,Z., Li,W., Ni,C. and Fang,Q.
  TITLE     Achaete-scute complex-like 2 regulated inflammatory mechanism
            through Toll-like receptor 4 activating in stomach adenocarcinoma
  JOURNAL   World J Surg Oncol 20 (1), 266 (2022)
   PUBMED   36008864
  REMARK    GeneRIF: Achaete-scute complex-like 2 regulated inflammatory
            mechanism through Toll-like receptor 4 activating in stomach
            adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Shang,Y., Jiang,T., Ran,L., Hu,W., Wu,Y., Ye,J., Peng,Z., Chen,L.
            and Wang,R.
  TITLE     TET2-BCLAF1 transcription repression complex epigenetically
            regulates the expression of colorectal cancer gene Ascl2 via
            methylation of its promoter
  JOURNAL   J Biol Chem 298 (7), 102095 (2022)
   PUBMED   35660018
  REMARK    GeneRIF: TET2-BCLAF1 transcription repression complex
            epigenetically regulates the expression of colorectal cancer gene
            Ascl2 via methylation of its promoter.
REFERENCE   3  (bases 1 to 1485)
  AUTHORS   Wu,L., Sun,S., Qu,F., Liu,X., Sun,M., Pan,Y., Zheng,Y. and Su,G.
  TITLE     ASCL2 Affects the Efficacy of Immunotherapy in Colon Adenocarcinoma
            Based on Single-Cell RNA Sequencing Analysis
  JOURNAL   Front Immunol 13, 829640 (2022)
   PUBMED   35774798
  REMARK    GeneRIF: ASCL2 Affects the Efficacy of Immunotherapy in Colon
            Adenocarcinoma Based on Single-Cell RNA Sequencing Analysis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1485)
  AUTHORS   Varberg,K.M., Iqbal,K., Muto,M., Simon,M.E., Scott,R.L., Kozai,K.,
            Choudhury,R.H., Aplin,J.D., Biswell,R., Gibson,M., Okae,H.,
            Arima,T., Vivian,J.L., Grundberg,E. and Soares,M.J.
  TITLE     ASCL2 reciprocally controls key trophoblast lineage decisions
            during hemochorial placenta development
  JOURNAL   Proc Natl Acad Sci U S A 118 (10) (2021)
   PUBMED   33649217
  REMARK    GeneRIF: ASCL2 reciprocally controls key trophoblast lineage
            decisions during hemochorial placenta development.
REFERENCE   5  (bases 1 to 1485)
  AUTHORS   Yang,Q., Huang,G., Li,L., Li,E. and Xu,L.
  TITLE     Potential Mechanism of Immune Evasion Associated with the Master
            Regulator ASCL2 in Microsatellite Stability in Colorectal Cancer
  JOURNAL   J Immunol Res 2021, 5964752 (2021)
   PUBMED   33628843
  REMARK    GeneRIF: Potential Mechanism of Immune Evasion Associated with the
            Master Regulator ASCL2 in Microsatellite Stability in Colorectal
            Cancer.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1485)
  AUTHORS   Westerman,B.A., Poutsma,A., Looijenga,L.H., Wouters,D., van
            Wijk,I.J. and Oudejans,C.B.
  TITLE     The Human Achaete Scute Homolog 2 gene contains two promotors,
            generating overlapping transcripts and encoding two proteins with
            different nuclear localization
  JOURNAL   Placenta 22 (6), 511-518 (2001)
   PUBMED   11440538
REFERENCE   7  (bases 1 to 1485)
  AUTHORS   Onions,J., Hermann,S. and Grundstrom,T.
  TITLE     A novel type of calmodulin interaction in the inhibition of basic
            helix-loop-helix transcription factors
  JOURNAL   Biochemistry 39 (15), 4366-4374 (2000)
   PUBMED   10757985
REFERENCE   8  (bases 1 to 1485)
  AUTHORS   Miyamoto,T., Jinno,Y., Miura,K., Sengoku,K., Soejima,H., Yun,K.,
            Yaginuma,Y., Niikawa,N. and Ishikawa,M.
  TITLE     A SacII polymorphism in the human ASCL2 (HASH2) gene region
  JOURNAL   J Hum Genet 43 (1), 69-70 (1998)
   PUBMED   9610003
REFERENCE   9  (bases 1 to 1485)
  AUTHORS   Alders,M., Hodges,M., Hadjantonakis,A.K., Postmus,J., van Wijk,I.,
            Bliek,J., de Meulemeester,M., Westerveld,A., Guillemot,F.,
            Oudejans,C., Little,P. and Mannens,M.
  TITLE     The human Achaete-Scute homologue 2 (ASCL2,HASH2) maps to
            chromosome 11p15.5, close to IGF2 and is expressed in extravillus
            trophoblasts
  JOURNAL   Hum Mol Genet 6 (6), 859-867 (1997)
   PUBMED   9175731
REFERENCE   10 (bases 1 to 1485)
  AUTHORS   Miyamoto,T., Jinno,Y., Sasaki,T., Ikeda,Y., Masuzaki,H., Niikawa,N.
            and Ishikawa,M.
  TITLE     Genomic cloning and localization to chromosome 11p15.5 of the human
            achaete-scute homolog 2 (ASCL2)
  JOURNAL   Cytogenet Cell Genet 73 (4), 312-314 (1996)
   PUBMED   8751384
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC129929.5.
            
            On Jun 26, 2020 this sequence version replaced NM_005170.2.
            
            Summary: This gene is a member of the basic helix-loop-helix (BHLH)
            family of transcription factors. It activates transcription by
            binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH
            proteins is required for efficient DNA binding. Involved in the
            determination of the neuronal precursors in the peripheral nervous
            system and the central nervous system. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC057801.1, SRR14038193.9006.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000331289.5/ ENSP00000332293.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-856               AC129929.5         116509-117364
            857-1485            AC129929.5         117971-118599
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.5"
     gene            1..1485
                     /gene="ASCL2"
                     /gene_synonym="ASH2; bHLHa45; HASH2; MASH2"
                     /note="achaete-scute family bHLH transcription factor 2"
                     /db_xref="GeneID:430"
                     /db_xref="HGNC:HGNC:739"
                     /db_xref="MIM:601886"
     exon            1..856
                     /gene="ASCL2"
                     /gene_synonym="ASH2; bHLHa45; HASH2; MASH2"
                     /inference="alignment:Splign:2.1.0"
     CDS             257..838
                     /gene="ASCL2"
                     /gene_synonym="ASH2; bHLHa45; HASH2; MASH2"
                     /note="mammalian achaete/scute homologue 2; class A basic
                     helix-loop-helix protein 45; achaete-scute complex-like 2;
                     achaete-scute complex homolog 2"
                     /codon_start=1
                     /product="achaete-scute homolog 2"
                     /protein_id="NP_005161.1"
                     /db_xref="CCDS:CCDS7732.1"
                     /db_xref="GeneID:430"
                     /db_xref="HGNC:HGNC:739"
                     /db_xref="MIM:601886"
                     /translation="MDGGTLPRSAPPAPPVPVGCAARRRPASPELLRCSRRRRPATAE
                     TGGGAAAVARRNERERNRVKLVNLGFQALRQHVPHGGASKKLSKVETLRSAVEYIRAL
                     QRLLAEHDAVRNALAGGLRPQAVRPSAPRGPPGTTPVAASPSRASSSPGRGGSSEPGS
                     PRSAYSSDDSGCEGALSPAERELLDFSSWLGGY"
     exon            857..1485
                     /gene="ASCL2"
                     /gene_synonym="ASH2; bHLHa45; HASH2; MASH2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1262..1267
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASCL2"
                     /gene_synonym="ASH2; bHLHa45; HASH2; MASH2"
                     /note="hexamer: AATAAA"
     polyA_site      1291
                     /gene="ASCL2"
                     /gene_synonym="ASH2; bHLHa45; HASH2; MASH2"
                     /note="major polyA site"
     regulatory      1467..1472
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASCL2"
                     /gene_synonym="ASH2; bHLHa45; HASH2; MASH2"
                     /note="hexamer: AATAAA"
     polyA_site      1485
                     /gene="ASCL2"
                     /gene_synonym="ASH2; bHLHa45; HASH2; MASH2"
                     /note="major polyA site"
ORIGIN      
        1 ggagacctgg gagagtccgg ccgcacgcgc gggacacgag cgtcccacgc tccctggcgc
       61 gtacggcctg ccaccactag gcctcctatc cccgggctcc agacgaccta ggacgcgtgc
      121 cctggggagt tgcctggcgg cgccgtgcca gaagccccct tggggcgcca cagttttccc
      181 cgtcgcctcc ggttcctctg cctgcacctt cctgcggcgc gccgggacct ggagcgggcg
      241 ggtggatgca ggcgcgatgg acggcggcac actgcccagg tccgcgcccc ctgcgccccc
      301 cgtccctgtc ggctgcgctg cccggcggag acccgcgtcc ccggaactgt tgcgctgcag
      361 ccggcggcgg cgaccggcca ccgcagagac cggaggcggc gcagcggccg tagcgcggcg
      421 caatgagcgc gagcgcaacc gcgtgaagct ggtgaacttg ggcttccagg cgctgcggca
      481 gcacgtgccg cacggcggcg ccagcaagaa gctgagcaag gtggagacgc tgcgctcagc
      541 cgtggagtac atccgcgcgc tgcagcgcct gctggccgag cacgacgccg tgcgcaacgc
      601 gctggcggga gggctgaggc cgcaggccgt gcggccgtct gcgccccgcg ggccgccagg
      661 gaccaccccg gtcgccgcct cgccctcccg cgcttcttcg tccccgggcc gcgggggcag
      721 ctcggagccc ggctccccgc gttccgccta ctcgtcggac gacagcggct gcgaaggcgc
      781 gctgagtcct gcggagcgcg agctactcga cttctccagc tggttagggg gctactgagc
      841 gccctcgacc tatgagcctc agccccggaa gccgagcgag cggccggcgc gctcatcgcc
      901 ggggagcccg ccaggtggac cggcccgcgc tccgccccca gcgagccggg gacccaccca
      961 ccaccccccg caccgccgac gccgcctcgt tcgtccggcc cagcctgacc aatgccgcgg
     1021 tggaaacggg cttggagctg gccccataag ggctggcggc ttcctccgac gccgcccctc
     1081 cccacagctt ctcgactgca gtggggcggg gggcaccaac acttggagat ttttccggag
     1141 gggagaggat tttctaaggg cacagagaat ccattttcta cacattaact tgagctgctg
     1201 gagggacact gctggcaaac ggagacctat ttttgtacaa agaacccttg acctggggcg
     1261 taataaagat gacctggacc cctgccccca ctatctggag ttttccatgc tggccaagat
     1321 ctggacacga gcagtccctg aggggcgggg tccctggcgt gaggcccccg tgacagccca
     1381 ccctggggtg ggtttgtggg cactgctgct ctgctaggga gaagcctgtg tggggcacac
     1441 ctcttcaagg gagcgtgaac tttataaata aatcagttct gttta
//
