LOCUS       NM_024095               2534 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens ankyrin repeat and SOCS box containing 8 (ASB8),
            transcript variant 2, mRNA.
ACCESSION   NM_024095
VERSION     NM_024095.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Guo,Y., Li,R., Tan,Z., Shi,J., Fu,Y., Song,Y., Zhu,M., Zhang,L. and
            Huang,J.
  TITLE     E3 ubiquitin ligase ASB8 negatively regulates interferon via
            regulating TBK1/IKKi homeostasis
  JOURNAL   Mol Immunol 121, 195-203 (2020)
   PUBMED   32298923
  REMARK    GeneRIF: E3 ubiquitin ligase ankyrin repeat and SOCS box-containing
            8(ASB8) interacted with TBK1/IKKi and phosphorylation modification
            of ASB8 at site of Ser17 to further strengthen its ubiquitination
            activity were verified. Phosphorylated ASB8 accelerates K48-linked
            ubiquitination and degradation of TBK1/IKKi, which further reduces
            phosphorylation level of IRF3 and inhibits production of IFN-beta.
REFERENCE   3  (bases 1 to 2534)
  AUTHORS   Liu,Y., Li,J., Zhang,F., Qin,W., Yao,G., He,X., Xue,P., Ge,C.,
            Wan,D. and Gu,J.
  TITLE     Molecular cloning and characterization of the human ASB-8 gene
            encoding a novel member of ankyrin repeat and SOCS box containing
            protein family
  JOURNAL   Biochem Biophys Res Commun 300 (4), 972-979 (2003)
   PUBMED   12559969
  REMARK    GeneRIF: molecular cloning and characterization of the human ASB-8
            gene
REFERENCE   4  (bases 1 to 2534)
  AUTHORS   Kile,B.T., Schulman,B.A., Alexander,W.S., Nicola,N.A., Martin,H.M.
            and Hilton,D.J.
  TITLE     The SOCS box: a tale of destruction and degradation
  JOURNAL   Trends Biochem Sci 27 (5), 235-241 (2002)
   PUBMED   12076535
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BM837218.1, BC001321.1 and AF464877.1.
            
            On Nov 22, 2018 this sequence version replaced NM_024095.4.
            
            Transcript Variant: This variant (2) lacks an alternate exon in the
            5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same
            isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.215110.1,
                                           SRR1163655.98531.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000317697.8/ ENSP00000320893.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-556               BM837218.1         81-636
            557-1664            BC001321.1         544-1651
            1665-2534           AF464877.1         1636-2505
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.11"
     gene            1..2534
                     /gene="ASB8"
                     /gene_synonym="PP14212"
                     /note="ankyrin repeat and SOCS box containing 8"
                     /db_xref="GeneID:140461"
                     /db_xref="HGNC:HGNC:17183"
                     /db_xref="MIM:615053"
     exon            1..57
                     /gene="ASB8"
                     /gene_synonym="PP14212"
                     /inference="alignment:Splign:2.1.0"
     exon            58..219
                     /gene="ASB8"
                     /gene_synonym="PP14212"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..957
                     /gene="ASB8"
                     /gene_synonym="PP14212"
                     /note="isoform a is encoded by transcript variant 2;
                     ankyrin repeat and SOCS box protein 8"
                     /codon_start=1
                     /product="ankyrin repeat and SOCS box protein 8 isoform a"
                     /protein_id="NP_077000.1"
                     /db_xref="CCDS:CCDS8761.1"
                     /db_xref="GeneID:140461"
                     /db_xref="HGNC:HGNC:17183"
                     /db_xref="MIM:615053"
                     /translation="MSSSMWYIMQSIQSKYSLSERLIRTIAAIRSFPHDNVEDLIRGG
                     ADVNCTHGTLKPLHCACMVSDADCVELLLEKGAEVNALDGYNRTALHYAAEKDEACVE
                     VLLEYGANPNALDGNRDTPLHWAAFKNNAECVRALLESGASVNALDYNNDTPLSWAAM
                     KGNLESVSILLDYGAEVRVINLIGQTPISRLVALLVRGLGTEKEDSCFELLHRAVGHF
                     ELRKNGTMPREVARDPQLCEKLTVLCSAPGTLKTLARYAVRRSLGLQYLPDAVKGLPL
                     PASLKEYLLLLE"
     exon            220..324
                     /gene="ASB8"
                     /gene_synonym="PP14212"
                     /inference="alignment:Splign:2.1.0"
     exon            325..2534
                     /gene="ASB8"
                     /gene_synonym="PP14212"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2514..2519
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASB8"
                     /gene_synonym="PP14212"
                     /note="hexamer: AATAAA"
     polyA_site      2534
                     /gene="ASB8"
                     /gene_synonym="PP14212"
                     /note="major polyA site"
ORIGIN      
        1 gcttccgggt cacgccttga cagcggcttt caacccccac ctcagcccag caattcggca
       61 gtttggagca tgtgaacacc ttgagccttg atgagttcca gtatgtggta tattatgcag
      121 agcattcaga gcaaatactc tctctccgag cgcttaatcc gaacaattgc tgccatccgt
      181 tccttcccac atgataatgt agaggacctc atcagagggg gagcagatgt gaactgcact
      241 catggcacac tgaagccctt gcactgtgcc tgtatggtgt cagatgctga ctgtgtggag
      301 ttacttctgg aaaaaggagc cgaggtgaat gccctggatg ggtataaccg aacagccctc
      361 cactatgcag cagagaaaga tgaggcttgt gtggaggtcc tattggagta tggtgcaaac
      421 cccaatgctt tggatggcaa cagagatacc ccacttcact gggcagcctt taagaacaat
      481 gctgagtgtg tgcgggctct cctagagagc ggggcctctg tcaatgccct ggattacaac
      541 aatgatacac cgctcagctg ggctgccatg aagggaaatc ttgagagtgt cagcatcctt
      601 ctggattatg gcgcagaggt cagagtcatc aacctaatag gccagacacc catctcccgc
      661 ctggtggctc tgctagtcag gggacttgga acagagaaag aggactcttg ctttgagctc
      721 ctccacagag ctgttggaca ctttgaattg aggaaaaatg gcaccatgcc acgagaggtg
      781 gccagagacc cgcagctatg tgaaaaactg actgttctgt gctcagctcc aggaactcta
      841 aaaacactcg ctcgctatgc cgtgcgccgt agcctgggac tccagtatct ccccgatgca
      901 gtgaagggcc ttccactgcc agcttctttg aaggaatacc tgttactttt agaatagccg
      961 gagaagatgt ttgcaccatc gtgcaggcag ctctgggtga ggttgtccct gcagtactcc
     1021 ttgtcacaga aaacagaaaa acagttgttc ctgttgtgtg gtttatagat ttcgaagcaa
     1081 catgtcacaa caataacctc catagcacct ccccttccca aaccaaacaa cccaacaaaa
     1141 aaaatccctc acttttgttt tctgtttatt gcttacctgg ctttttatat tgcattttgc
     1201 aaaagaagag gtctccctca atcctcccct ttagggaagg agtcaacagt gtaactaaat
     1261 ttctctagga agatggaaag tacttaaata atgtgtgtgt ggttttcctt tggggacgtg
     1321 gttaacggtc cagaagaatc ccttctagaa agcattttag gccagccatg gtggctcacg
     1381 tctgtaatcc caggactttg ggaggctgag gcaggtggat cacctgaggt caggagttcg
     1441 agcccagcct gaccaatatg atgaaacccc gtctctacta aaaatacaaa aattagctgg
     1501 gcatggtggc atgcgcctgt aatcccagct actcaggagg ctgagacaga agaatcgctt
     1561 gaacctgtga ggcagaggtt gcagtgagcc aagatcgcgc cattgcactc cagcctggac
     1621 aacaagagca aaactgtctc aaaaaaaaaa aaaaaaaaaa aaaaccattt taattgatct
     1681 gtgaaaaaac ttaagaaaat cacaatttca gctaacagca attgtgtccc aaagatgaag
     1741 atactataac ctcaaatggt gcagatccag aactgggctg gatgacatcc ctactgtgcc
     1801 atgtcctggg gcatttggaa gggactggac ctctttcccc tcatcaaagg aaacagcagt
     1861 ctttgcctct ttctgttggt tgtgcccaag ggctacagta gctctgaaat aacaagagct
     1921 ctgtaataac agtaataaat agctctgaaa taacagtcct aagaactcct aaagtcctga
     1981 gaacttttct tgtaatgcag ctttttctct tcctgagaaa cagtgtgttc taatgggatt
     2041 cccaggcagt tcctacacct acggtgtgtg ttccagcagg gaggagttat gggctgggct
     2101 gccttttccc atgggtcttc attcccaatg gaaagttcac tctgcttagt ttggaattat
     2161 ttttctttca gttgttctgg aacctttgct ttttattgat ttatacaata caattggtgg
     2221 gagggtggac ttgggatggg agtgggaaaa gcatgtaaga gctccttttg tgatggtcca
     2281 tctacccaaa agagatctgc tttagtgaac gatactcttt catttttcta aattagatca
     2341 agttgttatt gattttagat gacttgtatg caaatttgaa aaactttttt ttttaaagct
     2401 gattgggaac tacaaacaat gaatggaatc tactgacaca gctaattgga aaacagatgt
     2461 cttcttctgt cctattgatg ctggtgttta aaaaacatca cttaaaaaaa aagaataaat
     2521 agttctaaaa gcaa
//
