LOCUS       NM_003887               5665 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
            (ASAP2), transcript variant 1, mRNA.
ACCESSION   NM_003887
VERSION     NM_003887.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 5665)
  AUTHORS   Fujii,A., Masuda,T., Iwata,M., Tobo,T., Wakiyama,H., Koike,K.,
            Kosai,K., Nakano,T., Kuramitsu,S., Kitagawa,A., Sato,K.,
            Kouyama,Y., Shimizu,D., Matsumoto,Y., Utsunomiya,T., Ohtsuka,T.,
            Yamanishi,Y., Nakamura,M. and Mimori,K.
  TITLE     The novel driver gene ASAP2 is a potential druggable target in
            pancreatic cancer
  JOURNAL   Cancer Sci 112 (4), 1655-1668 (2021)
   PUBMED   33605496
  REMARK    GeneRIF: The novel driver gene ASAP2 is a potential druggable
            target in pancreatic cancer.
REFERENCE   4  (bases 1 to 5665)
  AUTHORS   Kennedy,S.A., Jarboui,M.A., Srihari,S., Raso,C., Bryan,K.,
            Dernayka,L., Charitou,T., Bernal-Llinares,M., Herrera-Montavez,C.,
            Krstic,A., Matallanas,D., Kotlyar,M., Jurisica,I., Curak,J.,
            Wong,V., Stagljar,I., LeBihan,T., Imrie,L., Pillai,P., Lynn,M.A.,
            Fasterius,E., Al-Khalili Szigyarto,C., Breen,J., Kiel,C.,
            Serrano,L., Rauch,N., Rukhlenko,O., Kholodenko,B.N.,
            Iglesias-Martinez,L.F., Ryan,C.J., Pilkington,R., Cammareri,P.,
            Sansom,O., Shave,S., Auer,M., Horn,N., Klose,F., Ueffing,M.,
            Boldt,K., Lynn,D.J. and Kolch,W.
  TITLE     Extensive rewiring of the EGFR network in colorectal cancer cells
            expressing transforming levels of KRASG13D
  JOURNAL   Nat Commun 11 (1), 499 (2020)
   PUBMED   31980649
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5665)
  AUTHORS   Seuter,S., Ryynanen,J. and Carlberg,C.
  TITLE     The ASAP2 gene is a primary target of 1,25-dihydroxyvitamin D3 in
            human monocytes and macrophages
  JOURNAL   J Steroid Biochem Mol Biol 144 Pt A, 12-18 (2014)
   PUBMED   23999061
REFERENCE   6  (bases 1 to 5665)
  AUTHORS   Kim,H. and Lee,Y.
  TITLE     Interaction of poly(A) polymerase with the 25-kDa subunit of
            cleavage factor I
  JOURNAL   Biochem Biophys Res Commun 289 (2), 513-518 (2001)
   PUBMED   11716503
REFERENCE   7  (bases 1 to 5665)
  AUTHORS   Uchida,H., Kondo,A., Yoshimura,Y., Mazaki,Y. and Sabe,H.
  TITLE     PAG3/Papalpha/KIAA0400, a GTPase-activating protein for
            ADP-ribosylation factor (ARF), regulates ARF6 in Fcgamma
            receptor-mediated phagocytosis of macrophages
  JOURNAL   J Exp Med 193 (8), 955-966 (2001)
   PUBMED   11304556
REFERENCE   8  (bases 1 to 5665)
  AUTHORS   Kondo,A., Hashimoto,S., Yano,H., Nagayama,K., Mazaki,Y. and Sabe,H.
  TITLE     A new paxillin-binding protein, PAG3/Papalpha/KIAA0400, bearing an
            ADP-ribosylation factor GTPase-activating protein activity, is
            involved in paxillin recruitment to focal adhesions and cell
            migration
  JOURNAL   Mol Biol Cell 11 (4), 1315-1327 (2000)
   PUBMED   10749932
REFERENCE   9  (bases 1 to 5665)
  AUTHORS   Andreev,J., Simon,J.P., Sabatini,D.D., Kam,J., Plowman,G.,
            Randazzo,P.A. and Schlessinger,J.
  TITLE     Identification of a new Pyk2 target protein with Arf-GAP activity
  JOURNAL   Mol Cell Biol 19 (3), 2338-2350 (1999)
   PUBMED   10022920
REFERENCE   10 (bases 1 to 5665)
  AUTHORS   Brown,M.T., Andrade,J., Radhakrishna,H., Donaldson,J.G.,
            Cooper,J.A. and Randazzo,P.A.
  TITLE     ASAP1, a phospholipid-dependent arf GTPase-activating protein that
            associates with and is phosphorylated by Src
  JOURNAL   Mol Cell Biol 18 (12), 7038-7051 (1998)
   PUBMED   9819391
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB007860.1, AC079782.6 and
            AA918883.1.
            
            On Nov 23, 2018 this sequence version replaced NM_003887.2.
            
            Summary: This gene encodes a multidomain protein containing an
            N-terminal alpha-helical region with a coiled-coil motif, followed
            by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin
            homology region, a proline-rich region, and a C-terminal Src
            homology 3 (SH3) domain. The protein localizes in the Golgi
            apparatus and at the plasma membrane, where it colocalizes with
            protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a
            stable complex with PYK2 in vivo. This interaction appears to be
            mediated by binding of its SH3 domain to the C-terminal
            proline-rich domain of PYK2. The encoded protein is tyrosine
            phosphorylated by activated PYK2. It has catalytic activity for
            class I and II ArfGAPs in vitro, and can bind the class III Arf
            ARF6 without immediate GAP activity. The encoded protein is
            believed to function as an ARF GAP that controls ARF-mediated
            vesicle budding when recruited to Golgi membranes. In addition, it
            functions as a substrate and downstream target for PYK2 and SRC, a
            pathway that may be involved in the regulation of vesicular
            transport. Multiple transcript variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, Sep 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB007860.1, SRR7346977.2974628.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000281419.8/ ENSP00000281419.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1396              AB007860.1         48-1443
            1397-1397           AC079782.6         81360-81360
            1398-5664           AB007860.1         1445-5711
            5665-5665           AA918883.1         13-13               c
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p25.1"
     gene            1..5665
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /note="ArfGAP with SH3 domain, ankyrin repeat and PH
                     domain 2"
                     /db_xref="GeneID:8853"
                     /db_xref="HGNC:HGNC:2721"
                     /db_xref="MIM:603817"
     exon            1..419
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     CDS             294..3314
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /note="isoform a is encoded by transcript variant 1;
                     centaurin, beta 3; PYK2 C terminus-associated protein;
                     arf-GAP with SH3 domain, ANK repeat and PH
                     domain-containing protein 2; pyk2 C-terminus-associated
                     protein; development and differentiation-enhancing factor
                     2; paxillin-associated protein with ARF GAP activity 3"
                     /codon_start=1
                     /product="arf-GAP with SH3 domain, ANK repeat and PH
                     domain-containing protein 2 isoform a"
                     /protein_id="NP_003878.1"
                     /db_xref="CCDS:CCDS1661.1"
                     /db_xref="GeneID:8853"
                     /db_xref="HGNC:HGNC:2721"
                     /db_xref="MIM:603817"
                     /translation="MPDQISVSEFVAETHEDYKAPTASSFTTRTAQCRNTVAAIEEAL
                     DVDRMVLYKMKKSVKAINSSGLAHVENEEQYTQALEKFGGNCVCRDDPDLGSAFLKFS
                     VFTKELTALFKNLIQNMNNIISFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKITKI
                     EKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKIKKGVDLLQ
                     NLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTIKQAQDEERRQLIQLRDILKSA
                     LQVEQKEDSQIRQSTAYSLHQPQGNKEHGTERNGSLYKKSDGIRKVWQKRKCSVKNGF
                     LTISHGTANRPPAKLNLLTCQVKTNPEEKKCFDLISHDRTYHFQAEDEQECQIWMSVL
                     QNSKEEALNNAFKGDDNTGENNIVQELTKEIISEVQRMTGNDVCCDCGAPDPTWLSTN
                     LGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELLLAKNIGNAGFNEIMECCLPAE
                     DSVKPNPGSDMNARKDYITAKYIERRYARKKHADNAAKLHSLCEAVKTRDIFGLLQAY
                     ADGVDLTEKIPLANGHEPDETALHLAVRSVDRTSLHIVDFLVQNSGNLDKQTGKGSTA
                     LHYCCLTDNAECLKLLLRGKASIEIANESGETPLDIAKRLKHEHCEELLTQALSGRFN
                     SHVHVEYEWRLLHEDLDESDDDMDEKLQPSPNRREDRPISFYQLGSNQLQSNAVSLAR
                     DAANLAKEKQRAFMPSILQNETYGALLSGSPPPAQPAAPSTTSAPPLPPRNVGKVQTA
                     SSANTLWKTNSVSVDGGSRQRSSSDPPAVHPPLPPLRVTSTNPLTPTPPPPVAKTPSV
                     MEALSQPSKPAPPGISQIRPPPLPPQPPSRLPQKKPAPGADKSTPLTNKGQPRGPVDL
                     SATEALGPLSNAMVLQPPAPMPRKSQATKLKPKRVKALYNCVADNPDELTFSEGDVII
                     VDGEEDQEWWIGHIDGDPGRKGAFPVSFVHFIAD"
     exon            420..492
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            493..638
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            639..713
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            714..763
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            764..893
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            894..979
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1055
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1142
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1246
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1316
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1404
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1405..1453
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1620
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1754
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1849
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1850..2039
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2040..2125
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2126..2241
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2242..2309
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2310..2423
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2424..2676
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2677..2811
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2812..2977
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2978..3027
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            3028..3116
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            3117..3239
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            3240..5665
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5645..5650
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /note="hexamer: AATAAA"
     polyA_site      5665
                     /gene="ASAP2"
                     /gene_synonym="AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;
                     SHAG1"
                     /note="major polyA site"
ORIGIN      
        1 agttcccgcc ggctgtgcgc gcccgcctcg gggctcgctc tgagaggacg cggcggcagc
       61 ggactcggag ccctcggcgc gcaggcgggc ggaccggccg agctgcgcgg ggctgcgcgc
      121 cgcccctgct ccgccgccag gccccgcgcg gctcccgcgc ccggcgctcc cctttgtccg
      181 cgggccggag cggcggcggc agcggcggtg tccgagcggc ggtcggagcc tgctgcggca
      241 gttgaggcgg cggcgcccct gcggctgtgc gccagcgccc tcgcgccgag gcgatgccgg
      301 accagatctc cgtgtcggaa ttcgtggccg agacccatga ggactacaag gcgcccacgg
      361 cctccagctt caccacccgc acggcgcagt gccggaacac tgtggcggcc atcgaggagg
      421 ctttggacgt ggaccggatg gttctttaca aaatgaagaa atccgtgaaa gcaatcaaca
      481 gctctgggct ggctcacgtg gaaaatgaag agcagtacac ccaggctctg gagaagtttg
      541 gcggcaactg tgtatgcaga gatgacccag atttaggaag tgcgttcctg aagttctcag
      601 tgtttacaaa ggagttgaca gcacttttca aaaacctgat tcagaatatg aacaacataa
      661 tctccttccc tttggacagt ttgctgaagg gggacctgaa aggagtgaaa ggggatctga
      721 aaaagccttt tgataaagct tggaaggact atgaaacaaa aataaccaag atagaaaagg
      781 agaaaaagga acacgccaag ctccatggga tgattcggac tgaaataagc ggagcggaaa
      841 ttgccgaaga gatggaaaag gagaggcgct tcttccagct acagatgtgc gagtatctgc
      901 tgaaggtcaa cgaaatcaag attaaaaagg gagtagattt acttcagaat ctgatcaaat
      961 actttcatgc ccaatgcaat ttttttcagg atggactcaa agccgtggaa agcctcaaac
     1021 cttccattga aacgctgtct acggatcttc acacgatcaa acaggcccag gatgaagaaa
     1081 gaaggcagtt gatacagctt cgagatattt tgaaatccgc attgcaggtt gaacagaaag
     1141 aggactccca aattcgtcag agcacagctt atagcttaca tcagcctcag ggaaacaagg
     1201 aacatgggac cgagcggaac ggcagcctct acaagaagag tgacgggatc cgaaaagtgt
     1261 ggcagaaaag gaaatgttca gttaaaaatg gttttctgac catatcccat ggtaccgcta
     1321 accggcctcc tgcaaagctc aacctgctaa cctgccaggt gaagaccaac cctgaggaga
     1381 agaagtgctt tgacctcatt tcacatgaca gaacttacca ctttcaagct gaagatgaac
     1441 aggaatgtca aatatggatg tctgtgctgc aaaatagcaa agaagaagct ttaaacaatg
     1501 catttaaggg ggatgacaat actggagaaa ataacatcgt ccaagaactg acaaaggaga
     1561 tcatctcaga agtgcagagg atgacgggca atgacgtctg ctgtgactgt ggggcgccag
     1621 atcctacatg gctttccacc aacctgggca tcctgacctg catcgagtgt tccggaatcc
     1681 accgagagct gggggttcat tattccagga tgcagtccct gaccttagat gtactgggaa
     1741 catctgagct gctgctcgcc aagaatattg ggaatgcagg ctttaatgag atcatggaat
     1801 gttgcctacc agctgaggac tcagtcaaac ccaacccagg cagcgacatg aatgcaagaa
     1861 aggactacat cacagccaag tacatcgaga ggagatacgc aaggaagaag cacgcggata
     1921 acgcggcgaa gcttcacagt ctttgcgagg ccgtcaaaac gagagatatt tttggattgc
     1981 tccaagctta tgctgatggt gtggatctta cggaaaaaat cccactggcc aacggacatg
     2041 agccggatga aacggccctc caccttgcag tcagatccgt ggatcgaacc tctcttcaca
     2101 ttgtagactt tttagttcag aacagtggga acctggataa acagacaggg aaaggcagca
     2161 cagccctgca ctactgctgc ctgaccgaca atgccgagtg cctcaagttg ctcctgcggg
     2221 ggaaggcctc catcgagata gcaaacgagt caggagagac tccgctggac attgccaagc
     2281 gcctcaagca cgagcactgt gaggagctgc tgacccaagc cttatctgga agatttaatt
     2341 ctcacgttca cgttgaatat gaatggcgac tactccacga agacctggat gaaagtgatg
     2401 acgacatgga tgagaaattg cagcccagtc ccaaccggcg ggaagaccgg cccatcagct
     2461 tctaccagct gggctccaac cagcttcagt ctaacgctgt atctttggcc agagatgctg
     2521 caaaccttgc caaggagaag cagagggctt tcatgcccag catcttgcag aatgagactt
     2581 acggagccct cctgagtggc agcccacctc ccgcccagcc tgcagccccc agcaccacca
     2641 gcgccccccc gcttcctcca cggaatgttg gcaaagttca gacagcctcc tctgctaaca
     2701 ccctgtggaa gacaaactct gtaagtgtgg acggtggaag ccggcagcga tcttcgtcag
     2761 atccgccagc tgtccatcca ccgctgcccc ctcttcgcgt gacatctacc aatcccctga
     2821 cccccacgcc gcccccaccc gttgccaaga cgcccagcgt aatggaagcc ttgagccagc
     2881 cgagcaagcc tgccccgcct gggatctcac agatcaggcc cccacctctg cccccacagc
     2941 cgcccagccg cctcccgcag aagaagcctg cgccgggggc tgacaagtcc accccactga
     3001 ccaacaaagg ccaaccgaga ggacctgtgg atctctctgc aacggaagct ctgggtcctc
     3061 tgtccaatgc tatggtcctg cagccccctg cacccatgcc taggaagtcg caggcaacca
     3121 agttgaagcc taagcgggtg aaagcgctct ataactgtgt ggctgacaac cccgatgagc
     3181 tcaccttctc cgagggggat gtgatcatcg tggacgggga ggaggaccag gagtggtgga
     3241 ttggccacat tgatggagat cctggtcgca aaggcgcatt cccggtgtca tttgtgcact
     3301 ttatcgctga ctgaattgct actgaacaaa agcattaaca gttatgttcc tgtttcgtta
     3361 ttggtaccaa aactcttgcc agataaccag tttcatgaac tgtttgtatg gcagcccatg
     3421 ttctctaatg ccactgctct gttttaaaaa ctcagaggca atttttacat atcagtaatt
     3481 gtttttataa tttgtggttt tcatgaaaca ttgctatgca tttattagga aaaactgaat
     3541 ttcccaacag gtgaactgaa aagttatttt aactattata cataatcaag atcctgcctc
     3601 tacggaatta gctaaaccta aaaatgtttg cattaatgaa taaattcttc ctgcattcct
     3661 tggcccagtt ctggagttgg tgacctttat cacaattatt attttaggcg gccagtgaac
     3721 tgctgcttca gaagtccata gcccagctct gaactttctc gataaaatgc catcagttca
     3781 cctttaaaga cacacattcc tttgaaatcc acccagtgtt taaaaagcaa cttggaaatt
     3841 tacacattag cattgtactt tctagcccta atttgtgagg ttgcagctat cattatattc
     3901 tgcatgtatg tataacctgt tgtgaacaat catacttaac aaaactactg atggtttatg
     3961 acaacgtagg gtaactacag ttcattctgt tccaggttat ataaaactgc atttcctgaa
     4021 tttggttaaa aactaaggat gatggattgc aaaacagttc ttttaaatta gtttatatgc
     4081 tttaggtgtt ttggaatttg ccttcttgaa cttcctgagt cacacagaaa gcaactgtac
     4141 acagtagaat tctgtggcgc agaccatgct gtattaacac atcacttgct gtttcctact
     4201 gagtgtacca ctgccttccc ttctagccca ggagaatgtt tactcagttt agtgtcttgt
     4261 atttctataa tactccaaca ggaatggtag tcacactgtc ttgaaattga atctgtccat
     4321 ctgtttataa tcaagaacat atcagaaata tataggtccc aggtaatact cccaaacatc
     4381 ccacttttta ctgtttcagg ccatcatatc attcttaagc tacttggggt ggtagtagag
     4441 gattaggttg tctattataa aaccaaaact cattcgttta atgaacttga ctgtcatacc
     4501 tctatttagt aattgcgagg gtaagattca tagtaggaat attggaaatt ttggcactct
     4561 gagaataaat aggcatatga tacccacttg gacttttaac aaaagtaaag gaataaattt
     4621 gcatataggc ttggaaagtg aggcagcaat gctgttaact gcatttgttg tgatggtgca
     4681 tttgattgaa gcagcttgtc tttattatgc aagactgtgt agagtttttt ttttttttgg
     4741 cattgtactt tttgtttttg ttataaagga agacagaaca aactggaatg ttttatgatg
     4801 ttgtatagca atcgcttttt acctttcaaa gttccgggta aaaatgtgtt atatctgtag
     4861 ttttttgttt ttgttttttt ttaaagcact acatctgttt tcactaattg ttaatttctg
     4921 tttgaaccct tcatttaatt ttctcataga tttaagtaaa cagatgtatt ttgcacagtg
     4981 cacttatgtc tattttaaca atcctcctgc atctgtattt tatagtcagc cttttgacca
     5041 cctggtgcca gctatataag gaataaagtt gattcatatc aacattagaa ctccagtccc
     5101 aaactaatct gtcaggttca ctggtacata aatacctagg aaatattttt ccagtctaca
     5161 atttggtgct attgtgcagt aactaatagt actcttacca gaggagaaat tatattaacg
     5221 accctgctaa tatcctttct tagttatttg ctccttgcaa attaaaaaag caactaagag
     5281 aaagaaaaac attgtagata tctatttata tttaaagttt atgtttcatg aactgcagct
     5341 gcaggattct ggcattttgc atgccattct ccatcagatc tgggatgatg gctcagaaca
     5401 tgtacacaga ctaagagtaa ctgtgtgatc tgttaagggg tggataacat aatatgcagc
     5461 ttaggatgct attttgagat gtatgatatt cagttcattc acctgattac tttggttgca
     5521 gcacaactgt atatattgta taaccgaaat tgattatttt cattgtcctt aatgcagtga
     5581 tttataatta gagcatgttt aataagttta ctcttcttgt taactagtca tttgactgga
     5641 aaaaaataaa atacttttaa atgga
//
