LOCUS       NM_018482               6259 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens ArfGAP with SH3 domain, ankyrin repeat and PH domain 1
            (ASAP1), transcript variant 1, mRNA.
ACCESSION   NM_018482
VERSION     NM_018482.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Zhang,Y., Bai,H., Zhang,W., Gao,J., Gao,C., Deng,T., Liu,X.,
            Sun,X., Liu,Y., Wang,N. and Wu,Y.
  TITLE     miR-212/132 attenuates OVA-induced airway inflammation by
            inhibiting mast cells activation through MRGPRX2 and ASAP1
  JOURNAL   Exp Cell Res 433 (2), 113828 (2023)
   PUBMED   37875175
  REMARK    GeneRIF: miR-212/132 attenuates OVA-induced airway inflammation by
            inhibiting mast cells activation through MRGPRX2 and ASAP1.
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Park,H., Son,H., Cha,H., Song,K., Bang,S., Jee,S., Kim,H.,
            Myung,J., Shin,S.J., Cha,C., Chung,M.S. and Paik,S.
  TITLE     ASAP1 Expression in Invasive Breast Cancer and Its Prognostic Role
  JOURNAL   Int J Mol Sci 24 (18), 14355 (2023)
   PUBMED   37762658
  REMARK    GeneRIF: ASAP1 Expression in Invasive Breast Cancer and Its
            Prognostic Role.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6259)
  AUTHORS   Barbagallo,C., Stella,M., Di Mauro,S., Scamporrino,A.,
            Filippello,A., Scionti,F., Di Martino,M.T., Purrello,M., Ragusa,M.,
            Purrello,F. and Piro,S.
  TITLE     An Uncharacterised lncRNA Coded by the ASAP1 Locus Is Downregulated
            in Serum of Type 2 Diabetes Mellitus Patients
  JOURNAL   Int J Mol Sci 24 (17), 13485 (2023)
   PUBMED   37686290
  REMARK    GeneRIF: An Uncharacterised lncRNA Coded by the ASAP1 Locus Is
            Downregulated in Serum of Type 2 Diabetes Mellitus Patients.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6259)
  AUTHORS   Xie,W., Han,Z., Zuo,Z., Xin,D., Chen,H., Huang,J., Zhu,S., Lou,H.,
            Yu,Z., Chen,C., Chen,S., Hu,Y., Huang,J., Zhang,F., Ni,Z., Shen,X.,
            Xue,X. and Lin,K.
  TITLE     ASAP1 activates the IQGAP1/CDC42 pathway to promote tumor
            progression and chemotherapy resistance in gastric cancer
  JOURNAL   Cell Death Dis 14 (2), 124 (2023)
   PUBMED   36792578
  REMARK    GeneRIF: ASAP1 activates the IQGAP1/CDC42 pathway to promote tumor
            progression and chemotherapy resistance in gastric cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6259)
  AUTHORS   Cui,X., Yuan,T., Ning,P., Han,J., Liu,Y., Feng,J., Lian,F., Hao,M.,
            Dong,L., Hao,J., Xing,L. and Wu,C.
  TITLE     Polymorphisms in the ASAP1 and SP110 Genes and Its Association with
            the Susceptibility to Pulmonary Tuberculosis in a Mongolian
            Population
  JOURNAL   J Immunol Res 2022, 2713869 (2022)
   PUBMED   36249417
  REMARK    GeneRIF: Polymorphisms in the ASAP1 and SP110 Genes and Its
            Association with the Susceptibility to Pulmonary Tuberculosis in a
            Mongolian Population.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6259)
  AUTHORS   Oshiro,T., Koyama,S., Sugiyama,S., Kondo,A., Onodera,Y.,
            Asahara,T., Sabe,H. and Kikuchi,A.
  TITLE     Interaction of POB1, a downstream molecule of small G protein Ral,
            with PAG2, a paxillin-binding protein, is involved in cell
            migration
  JOURNAL   J Biol Chem 277 (41), 38618-38626 (2002)
   PUBMED   12149250
  REMARK    GeneRIF: These results suggest that POB1 interacts with PAG2
            through its proline-rich motif, thereby regulating cell migration
            (PAG2).
REFERENCE   7  (bases 1 to 6259)
  AUTHORS   Scott,M.P., Zappacosta,F., Kim,E.Y., Annan,R.S. and Miller,W.T.
  TITLE     Identification of novel SH3 domain ligands for the Src family
            kinase Hck. Wiskott-Aldrich syndrome protein (WASP),
            WASP-interacting protein (WIP), and ELMO1
  JOURNAL   J Biol Chem 277 (31), 28238-28246 (2002)
   PUBMED   12029088
REFERENCE   8  (bases 1 to 6259)
  AUTHORS   Liu,Y., Loijens,J.C., Martin,K.H., Karginov,A.V. and Parsons,J.T.
  TITLE     The association of ASAP1, an ADP ribosylation factor-GTPase
            activating protein, with focal adhesion kinase contributes to the
            process of focal adhesion assembly
  JOURNAL   Mol Biol Cell 13 (6), 2147-2156 (2002)
   PUBMED   12058076
REFERENCE   9  (bases 1 to 6259)
  AUTHORS   Furman,C., Short,S.M., Subramanian,R.R., Zetter,B.R. and
            Roberts,T.M.
  TITLE     DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that
            enhances cell motility through a GAP-dependent mechanism
  JOURNAL   J Biol Chem 277 (10), 7962-7969 (2002)
   PUBMED   11773070
  REMARK    GeneRIF: DEF-1 alters cell motility through the deactivation of
            ARF1. In contrast, the inhibition of cell spreading by DEF-1 was
            not dependent on GAP activity, indicating that spreading and
            motility are altered by DEF-1 through different pathways.
REFERENCE   10 (bases 1 to 6259)
  AUTHORS   Brown,M.T., Andrade,J., Radhakrishna,H., Donaldson,J.G.,
            Cooper,J.A. and Randazzo,P.A.
  TITLE     ASAP1, a phospholipid-dependent arf GTPase-activating protein that
            associates with and is phosphorylated by Src
  JOURNAL   Mol Cell Biol 18 (12), 7038-7051 (1998)
   PUBMED   9819391
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP005856.2, DA319233.1,
            BC137135.1, AB033075.1 and AI494058.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_018482.3.
            
            Summary: This gene encodes an ADP-ribosylation factor (ARF)
            GTPase-activating protein. The GTPase-activating activity is
            stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is
            greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe
            involved in regulation of membrane trafficking and cytoskeleton
            remodeling. Alternatively spliced transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Oct 2011].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript, and encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR3218372.153065.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000518721.6/ ENSP00000429900.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                AP005856.2         8759-8772           c
            15-217              DA319233.1         1-203
            218-5521            BC137135.1         1-5304
            5522-6256           AB033075.1         4741-5475
            6257-6259           AI494058.1         15-17               c
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.21-q24.22"
     gene            1..6259
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /note="ArfGAP with SH3 domain, ankyrin repeat and PH
                     domain 1"
                     /db_xref="GeneID:50807"
                     /db_xref="HGNC:HGNC:2720"
                     /db_xref="MIM:605953"
     exon            1..215
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            216..301
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     CDS             243..3632
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /note="isoform 1 is encoded by transcript variant 1;
                     centaurin, beta 4; arf-GAP with SH3 domain, ANK repeat and
                     PH domain-containing protein 1; DEF-1; PIP2-dependent ARF1
                     GAP; ARF GTPase-activating protein 1; development and
                     differentiation-enhancing factor 1; ADP-ribosylation
                     factor-directed GTPase-activating protein 1; 130 kDa
                     phosphatidylinositol 4,5-biphosphate-dependent ARF1
                     GTPase-activating protein; 130 kDa phosphatidylinositol
                     4,5-bisphosphate-dependent ARF1 GTPase-activating protein"
                     /codon_start=1
                     /product="arf-GAP with SH3 domain, ANK repeat and PH
                     domain-containing protein 1 isoform 1"
                     /protein_id="NP_060952.2"
                     /db_xref="CCDS:CCDS6362.1"
                     /db_xref="GeneID:50807"
                     /db_xref="HGNC:HGNC:2720"
                     /db_xref="MIM:605953"
                     /translation="MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTS
                     SFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKF
                     GSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKG
                     DLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQM
                     CEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIK
                     QTQDEEKKQLTALRDLIKSSLQLDQKEDSQSRQGGYSMHQLQGNKEYGSEKKGYLLKK
                     SDGIRKVWQRRKCSVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNR
                     TYHFQAEDEQDYVAWISVLTNSKEEALTMAFRGEQSAGENSLEDLTKAIIEDVQRLPG
                     NDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAK
                     NVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSTSSAKLNE
                     LLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLV
                     QNCGNLDKQTALGNTVLHYCSMYSKPECLKLLLRSKPTVDIVNQAGETALDIAKRLKA
                     TQCEDLLSQAKSGKFNPHVHVEYEWNLRQEEIDESDDDLDDKPSPIKKERSPRPQSFC
                     HSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPTTEAPPLPPRNA
                     GKGPTGPPSTLPLSTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAVP
                     WGNDGGPSSSSKTTNKFEGLSQQSSTSSAKTALGPRVLPKLPQKVALRKTDHLSLDKA
                     TIPPEIFQKSSQLAELPQKPPPGDLPPKPTELAPKPQIGDLPPKPGELPPKPQLGDLP
                     PKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQTGDVSPKAQQPSEVTLKSHPLDLSPNV
                     QSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTF
                     IEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD"
     exon            302..428
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            429..501
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            502..647
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            648..722
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            723..772
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            773..902
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            903..988
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1064
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1151
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1252
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1322
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1410
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1459
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1623
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1757
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1849
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            1850..2036
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            2037..2122
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            2123..2238
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            2239..2306
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            2307..2414
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            2415..2643
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            2644..2814
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            2815..2884
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            2885..2943
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            2944..3434
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            3435..3557
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     exon            3558..6259
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6232..6237
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /note="hexamer: AATAAA"
     polyA_site      6259
                     /gene="ASAP1"
                     /gene_synonym="AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P"
                     /note="major polyA site"
ORIGIN      
        1 acatggtcgc ccgccccttc ctgcgccgcg ctcggctccc gcgcctcgcc tggcctggcc
       61 gctgccgcgg gcgagcactg tgcgctgggc agccccgggc ccgcgcctcc cgcccagccg
      121 gcgcttcccg gtcgcagttc gctttcccac ccgagagccg cgagcgcccg gccgaacccg
      181 cgatggaata atgcccagcg gcccgccagg tcccggtcgt tttctgatgt gacggctgag
      241 acatgagatc ttcagcctcc aggctctcca gtttttcgtc gagagattca ctatggaatc
      301 ggatgccgga ccagatctct gtctcggagt tcatcgccga gaccaccgag gactacaact
      361 cgcccaccac gtccagcttc accacgcggc tgcacaactg caggaacacc gtcacgctgc
      421 tggaggaggc tctagaccaa gatagaacag cccttcagaa agtgaagaag tctgtaaaag
      481 caatatataa ttctggtcaa gatcatgtac aaaatgaaga aaactatgca caagttcttg
      541 ataagtttgg gagtaatttt ttaagtcgag acaaccccga ccttggcacc gcgtttgtca
      601 agttttctac tcttacaaag gaactgtcca cactgctgaa aaatctgctc cagggtttga
      661 gccacaatgt gatcttcacc ttggattctt tgttaaaagg agacctaaag ggagtcaaag
      721 gagatctcaa gaagccattt gacaaagcct ggaaagatta tgagacaaag tttacaaaaa
      781 ttgagaaaga gaaaagagag cacgcaaaac aacatgggat gatccgcaca gagataacag
      841 gagctgagat tgcggaagaa atggagaagg aaaggcgcct ctttcagctc caaatgtgtg
      901 aatatctcat taaagttaat gaaatcaaga ccaaaaaggg tgtggatctg ctgcagaatc
      961 ttataaagta ttaccatgca cagtgcaatt tctttcaaga tggcttgaaa acagctgata
     1021 agttgaaaca gtacattgaa aaactggctg ctgatttata taatataaaa cagacccagg
     1081 atgaagaaaa gaaacagcta actgcactcc gagacttaat aaaatcctct cttcaactgg
     1141 atcagaaaga agattctcag agccggcaag gaggatacag catgcatcag ctccagggca
     1201 ataaggaata tggcagtgaa aagaaggggt acctgctaaa gaaaagtgac gggatccgga
     1261 aagtatggca gaggaggaag tgttcagtca agaatgggat tctgaccatc tcacatgcca
     1321 catctaacag gcaaccagcc aagttgaacc ttctcacctg ccaagtaaaa cctaatgccg
     1381 aagacaaaaa atcttttgac ctgatatcac ataatagaac atatcacttt caggcagaag
     1441 atgagcagga ttatgtagca tggatatcag tattgacaaa tagcaaagaa gaggccctaa
     1501 ccatggcctt ccgtggagag cagagtgcgg gagagaacag cctggaagac ctgacaaaag
     1561 ccattattga ggatgtccag cggctcccag ggaatgacat ttgctgcgat tgtggctcat
     1621 cagaacccac ctggctttca accaacttgg gtattttgac ctgtatagaa tgttctggca
     1681 tccataggga aatgggggtt catatttctc gcattcagtc tttggaacta gacaaattag
     1741 gaacttctga actcttgctg gccaagaatg taggaaacaa tagttttaat gatattatgg
     1801 aagcaaattt acccagcccc tcaccaaaac ccaccccttc aagtgatatg actgtacgaa
     1861 aagaatatat cactgcaaag tatgtagatc ataggttttc aaggaagacc tgttcaactt
     1921 catcagctaa actaaatgaa ttgcttgagg ccatcaaatc cagggattta cttgcactaa
     1981 ttcaagtcta tgcagaaggg gtagagctaa tggaaccact gctggaacct gggcaggagc
     2041 ttggggagac agcccttcac cttgccgtcc gaactgcaga tcagacatct ctccatttgg
     2101 ttgacttcct tgtacaaaac tgtgggaacc tggataagca gacggccctg ggaaacacag
     2161 ttctacacta ctgtagtatg tacagtaaac ctgagtgttt gaagcttttg ctcaggagca
     2221 agcccactgt ggatatagtt aaccaggctg gagaaactgc cctagacata gcaaagagac
     2281 taaaagctac ccagtgtgaa gatctgcttt cccaggctaa atctggaaag ttcaatccac
     2341 acgtccacgt agaatatgag tggaatcttc gacaggagga gatagatgag agcgatgatg
     2401 atctggatga caaaccaagc cctatcaaga aagagcgctc acccagacct cagagcttct
     2461 gccactcctc cagcatctcc ccccaggaca agctggcact gccaggattc agcactccaa
     2521 gggacaaaca gcggctctcc tatggagcct tcaccaacca gatcttcgtt tccacaagca
     2581 cagactcgcc cacatcacca accacggagg ctccccctct gcctcctagg aacgccggga
     2641 aaggtccaac tggcccacct tcaacactcc ctctaagcac ccagacctct agtggcagct
     2701 ccaccctatc caagaagagg cctcctcccc caccacccgg acacaagaga accctatccg
     2761 accctcccag cccactacct catgggcccc caaacaaagg cgcagttcct tggggtaacg
     2821 atgggggtcc atcctcttca agtaagacta caaacaagtt tgagggacta tcccagcagt
     2881 cgagcaccag ttctgcaaag actgcccttg gcccaagagt tcttcctaaa ctacctcaga
     2941 aagtggcact aaggaaaaca gatcatctct ccctagacaa agccaccatc ccgcccgaaa
     3001 tctttcagaa atcatcacag ttggcagagt tgccacaaaa gccaccacct ggagacctgc
     3061 ccccaaagcc cacagaactg gcccccaagc cccaaattgg agatttgccg cctaagccag
     3121 gagaactgcc ccccaaacca cagctggggg acctgccacc caaaccccaa ctctcagact
     3181 tacctcccaa accacagatg aaggacctgc cccccaaacc acagctggga gacctgctag
     3241 caaaatccca gactggagat gtctcaccca aggctcagca accctctgag gtcacactga
     3301 agtcacaccc attggatcta tccccaaatg tgcagtccag agacgccatc caaaagcaag
     3361 catctgaaga ctccaacgac ctcacgccta ctctgccaga gacgcccgta ccactgccca
     3421 gaaaaatcaa tacggggaaa aataaagtga ggcgagtgaa gaccatttat gactgccagg
     3481 cagacaacga tgacgagctc acattcatcg agggagaagt gattatcgtc acaggggaag
     3541 aggaccagga gtggtggatt ggccacatcg aaggacagcc tgaaaggaag ggggtctttc
     3601 cagtgtcctt tgttcatatc ctgtctgact agcaaaacgc agaaccttaa gattgtccac
     3661 atccttcatg caagactgct gccttcatgt aaccctgggc acagtgtgta tatagctgct
     3721 gttacagagt aagaaactca tggaagggcc acctcaggag ggggatataa tgtgtgttgt
     3781 aaatatcctg tggttttctg ccttcaccag tatgagggta gcctcggacc cggcgcgcct
     3841 tactggtttg ccaaagccat ccttggcatc tagcacttac atctctctat gctgttctac
     3901 aagcaaacaa acaaaaatag gagtatagga actgctggct ttgcaaatag aagtggtctc
     3961 cagcaaccgt tgaaaggcat agaattgact ctgttcctaa caatgcagta ttctcaattg
     4021 tgttactgaa aatgcaacat tagcaaagag gtgggttctg ttttccaggt gaaactttta
     4081 gctccatgac agaccagcct gtagttatct gtgtacacag tttacagcta caaaaaccta
     4141 ctttggtatt tattacagaa aagtgctcag ttaaatgtaa gtgttattcc ttcagcaaaa
     4201 tattcactga cccaaaactc tttatggcat tttacaatgc acacagcctc atgcaagttt
     4261 agacaagtgg atttatactg tcttatgagt gcccgcccct gatatattac ctcattatgc
     4321 aaaaataaca tatctttcat gactattttg acaaaagttt aaaacacata tgaagttcaa
     4381 atttcaggaa ccaaggactg ccagaaaata ttagcctcta cattacgcat gcatttagaa
     4441 gcttacctga aatctgcctt ttataaagga atagtatgga taagtggaat tgtacatttt
     4501 ttaaacttga ttgccattaa agcagaaatt ataaggttgc aacaatattt gtttctaatc
     4561 actggctttc tcaagagtat ggattgacat attgtgttat gaatgcacat ctctcagatg
     4621 tgttgaagca tccattgcat ccatttttta ttattttctt agttttgttc ttggacaaat
     4681 ttaaactttt aaaagattat tcaagatgaa tttaaaagtc aacccttcac acagtttccc
     4741 tactgtatgt agaatccagg tgctgaaacc aagtgtttct tttcccatgc tctttgttaa
     4801 accccaatta tagataattt ttccagtctt aagctctgtc caccttcaag tcaattcata
     4861 accaagtttt tgaacgctgc tatgaattgc actgtgaaaa gcactcttcc ctctcagttt
     4921 tcttttcatc ccagccatgt ttatcagatc cttaagaaca ttgtatttca gtcttttaca
     4981 tcagtctgaa ttttggaaaa gaatgcaata gttgtactcc acagtcagtg gaactgttcc
     5041 ctgagtccga ggctcatgtg tcattctggc actacatttg cttaaattgc tattttggca
     5101 acagcacaga aaactaatat ttttaagcag agaatcttgg caatgagtga gagatgttaa
     5161 tttcacagaa gcacaactcc caacccaacc cttaggaaaa gccctcttcc atcgttacag
     5221 tgctcagtga atattaattt agttctgctt aagtggttgc tatacaaact ttgaatagcc
     5281 acctaataaa taaaccttgc atgacaaacc tgcaaaatat tttatcagct gttattggaa
     5341 agtgatttta agcaattgct tcctcagtgt cagggcacat gtgaatttcc acaccaaaca
     5401 gagcatgagg aaccagttga catgctgggt tgtgactggc agctttagca gcctcggtac
     5461 tgaagccaca ccagtgtccg gatggaagtc tgcatctgag gttgctcagt gtcccggtca
     5521 ttcatttaca cattttaact tgcattaaag agctgttctt ttctgtggcc tagactcttt
     5581 tcactgatct caaaataaac tggttttttt caaaaaaaaa aaaaaaacaa aaacaaaaaa
     5641 aaaacacaaa agctgcatgt ctaaaattac atggagttag tgtctattct ttttcccctt
     5701 ttgcagcaac ttacacagca tttttaacac cttttttttc tagttttttt gttcggtttt
     5761 gttttccatc aggaatttga gttctctcta acccagctta ctgtgggaca taggaaaact
     5821 cagtagaaat acctttggtg atcttgttga gtttaagtct gatcttgatc ttaaactcag
     5881 taagccacta tctgcaattt tgtacattat atagtatttt gaagatatgg aaccttatga
     5941 aaaaaaaata gcaaattagt tctttttccc ccagagggga aagttatgtt ctgcaaatag
     6001 tgtgtgtctt attttactgt tgaacagcaa ttgctattta tttttttatt gcctagaact
     6061 tcaacatgtt gtataggaat cctgtagtgc cactagttaa atgccgaatt ctcatctgga
     6121 tgttaccatc aaacatcagt acacttgtca tttcacatgt gtttaatgtg acagtttttc
     6181 agtactgtat gtgttaattt ctactttttt taatatttaa aattgctttt aaataaacat
     6241 attctcagtt gatcccaaa
//
