LOCUS       NM_019893               4955 bp    mRNA    linear   PRI 08-FEB-2025
DEFINITION  Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript
            variant 1, mRNA.
ACCESSION   NM_019893 XM_011539970 XM_936277
VERSION     NM_019893.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Simoes,M., Saleh,A., Choi,Y.M., Airola,M.V., Haley,J.D. and
            Coant,N.
  TITLE     Measurement of neutral ceramidase activity in vitro and in vivo
  JOURNAL   Anal Biochem 643, 114577 (2022)
   PUBMED   35134389
  REMARK    GeneRIF: Measurement of neutral ceramidase activity in vitro and in
            vivo.
            Review article
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Zhu,H., Klement,J.D., Lu,C., Redd,P.S., Yang,D., Smith,A.D.,
            Poschel,D.B., Zou,J., Liu,D., Wang,P.G., Ostrov,D., Coant,N.,
            Hannun,Y.A., Colby,A.H., Grinstaff,M.W. and Liu,K.
  TITLE     Asah2 Represses the p53-Hmox1 Axis to Protect Myeloid-Derived
            Suppressor Cells from Ferroptosis
  JOURNAL   J Immunol 206 (6), 1395-1404 (2021)
   PUBMED   33547170
  REMARK    GeneRIF: Asah2 Represses the p53-Hmox1 Axis to Protect
            Myeloid-Derived Suppressor Cells from Ferroptosis.
REFERENCE   3  (bases 1 to 4955)
  AUTHORS   Coant,N. and Hannun,Y.A.
  TITLE     Neutral ceramidase: Advances in mechanisms, cell regulation, and
            roles in cancer
  JOURNAL   Adv Biol Regul 71, 141-146 (2019)
   PUBMED   30389354
  REMARK    GeneRIF: Neutral ceramidase: Advances in mechanisms, cell
            regulation, and roles in cancer.
            Review article
REFERENCE   4  (bases 1 to 4955)
  AUTHORS   Ohlsson,L., Palmberg,C., Duan,R.D., Olsson,M., Bergman,T. and
            Nilsson,A.
  TITLE     Purification and characterization of human intestinal neutral
            ceramidase
  JOURNAL   Biochimie 89 (8), 950-960 (2007)
   PUBMED   17475390
  REMARK    GeneRIF: the purification and characterization of neutral
            ceramidase from human ileostomy content, using
            octanoyl-[(14)C]sphingosine as substrate
REFERENCE   5  (bases 1 to 4955)
  AUTHORS   Galadari,S., Wu,B.X., Mao,C., Roddy,P., El Bawab,S. and Hannun,Y.A.
  TITLE     Identification of a novel amidase motif in neutral ceramidase
  JOURNAL   Biochem J 393 (Pt 3), 687-695 (2006)
   PUBMED   16229686
  REMARK    GeneRIF: the present study identified a novel amidase sequence
            containing a critical serine residue that may function as a
            nucleophile in the hydrolytic attack on the amide bond present in
            ceramide
REFERENCE   6  (bases 1 to 4955)
  AUTHORS   Hwang,Y.H., Tani,M., Nakagawa,T., Okino,N. and Ito,M.
  TITLE     Subcellular localization of human neutral ceramidase expressed in
            HEK293 cells
  JOURNAL   Biochem Biophys Res Commun 331 (1), 37-42 (2005)
   PUBMED   15845354
  REMARK    GeneRIF: Neutral ceramidase is localized mainly in plasma
            membranes.
REFERENCE   7  (bases 1 to 4955)
  AUTHORS   Choi,M.S., Anderson,M.A., Zhang,Z., Zimonjic,D.B., Popescu,N. and
            Mukherjee,A.B.
  TITLE     Neutral ceramidase gene: role in regulating ceramide-induced
            apoptosis
  JOURNAL   Gene 315, 113-122 (2003)
   PUBMED   14557071
REFERENCE   8  (bases 1 to 4955)
  AUTHORS   Mitsutake,S., Tani,M., Okino,N., Mori,K., Ichinose,S., Omori,A.,
            Iida,H., Nakamura,T. and Ito,M.
  TITLE     Purification, characterization, molecular cloning, and subcellular
            distribution of neutral ceramidase of rat kidney
  JOURNAL   J Biol Chem 276 (28), 26249-26259 (2001)
   PUBMED   11328816
REFERENCE   9  (bases 1 to 4955)
  AUTHORS   El Bawab,S., Birbes,H., Roddy,P., Szulc,Z.M., Bielawska,A. and
            Hannun,Y.A.
  TITLE     Biochemical characterization of the reverse activity of rat brain
            ceramidase. A CoA-independent and fumonisin B1-insensitive ceramide
            synthase
  JOURNAL   J Biol Chem 276 (20), 16758-16766 (2001)
   PUBMED   11278489
REFERENCE   10 (bases 1 to 4955)
  AUTHORS   El Bawab,S., Roddy,P., Qian,T., Bielawska,A., Lemasters,J.J. and
            Hannun,Y.A.
  TITLE     Molecular cloning and characterization of a human mitochondrial
            ceramidase
  JOURNAL   J Biol Chem 275 (28), 21508-21513 (2000)
   PUBMED   10781606
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL450382.7.
            
            On or before Jun 23, 2020 this sequence version replaced
            XM_011539970.2, NM_019893.3.
            
            Summary: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze
            hydrolysis of the N-acyl linkage of ceramide, a second messenger in
            a variety of cellular events, to produce sphingosine. Sphingosine
            exerts both mitogenic and apoptosis-inducing activities, and its
            phosphorylated form functions as an intra- and intercellular second
            messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed
            11328816]).[supplied by OMIM, Mar 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.468560.1,
                                           SRR11853560.3480.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000682911.1/ ENSP00000506746.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-122               AL450382.7         126670-126791       c
            123-285             AL450382.7         123759-123921       c
            286-518             AL450382.7         120497-120729       c
            519-668             AL450382.7         118477-118626       c
            669-845             AL450382.7         111163-111339       c
            846-973             AL450382.7         109700-109827       c
            974-1051            AL450382.7         108459-108536       c
            1052-1172           AL450382.7         93785-93905         c
            1173-1298           AL450382.7         90018-90143         c
            1299-1385           AL450382.7         88247-88333         c
            1386-1490           AL450382.7         86305-86409         c
            1491-1572           AL450382.7         86098-86179         c
            1573-1688           AL450382.7         81257-81372         c
            1689-1783           AL450382.7         80136-80230         c
            1784-1823           AL450382.7         78915-78954         c
            1824-1919           AL450382.7         78104-78199         c
            1920-2015           AL450382.7         74326-74421         c
            2016-2162           AL450382.7         72048-72194         c
            2163-2222           AL450382.7         67928-67987         c
            2223-2311           AL450382.7         64774-64862         c
            2312-4955           AL450382.7         60136-62779         c
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q11.23"
     gene            1..4955
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /note="N-acylsphingosine amidohydrolase 2"
                     /db_xref="GeneID:56624"
                     /db_xref="HGNC:HGNC:18860"
                     /db_xref="MIM:611202"
     exon            1..122
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            123..285
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..2501
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /EC_number="3.5.1.23"
                     /note="isoform a is encoded by transcript variant 1;
                     mitochondrial ceramidase; neutral ceramidase;
                     non-lysosomal ceramidase; neutral/alkaline ceramidase;
                     N-acylsphingosine amidohydrolase (non-lysosomal
                     ceramidase) 2; acylsphingosine deacylase 2"
                     /codon_start=1
                     /product="neutral ceramidase isoform a"
                     /protein_id="NP_063946.2"
                     /db_xref="CCDS:CCDS7239.2"
                     /db_xref="GeneID:56624"
                     /db_xref="HGNC:HGNC:18860"
                     /db_xref="MIM:611202"
                     /translation="MAKRTFSNLETFLIFLLVMMSAITVALLSLLFITSGTIENHKDL
                     GGHFFSTTQSPPATQGSTAAQRSTATQHSTATQSSTATQTSPVPLTPESPLFQNFSGY
                     HIGVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSIDI
                     GMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQ
                     TFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSN
                     TDKEMIVLKMVDLNGDDLGLISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQEKNKGY
                     LPGQGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTCPIGGPSMCIAKGPGQD
                     MFDSTQIIGRAMYQRAKELYASASQEVTGPLASAHQWVDMTDVTVWLNSTHASKTCKP
                     ALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHT
                     GELSKPHPWHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQAEFASHGMQNMT
                     VVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRNLAKAIATDTVAN
                     LSRGPEPPFFKQLIVPLIPSIVDRAPKGRTFGDVLQPAKPEYRVGEVAEVIFVGANPK
                     NSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYWHKGLLGLSNATVEWHIPDT
                     AQPGIYRIRYFGHNRKQDILKPAVILSFEGTSPAFEVVTI"
     exon            286..518
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            519..668
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            669..845
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            846..973
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1051
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1172
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1298
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1385
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1490
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1572
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1688
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            1689..1783
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            1784..1823
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1919
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..2015
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            2016..2162
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            2163..2222
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            2223..2311
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     exon            2312..4955
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4929..4934
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /note="hexamer: AATAAA"
     polyA_site      4955
                     /gene="ASAH2"
                     /gene_synonym="BCDase; HNAC1; LCDase; N-CDase; NCDase"
                     /note="major polyA site"
ORIGIN      
        1 agtaggtttc agaaagtgct gagaggtgta agcccagcaa cgtgcaaggg gaaaggggac
       61 aggattctgg atggccattt gcttcactgg gatgcaaaac ctcttttgag tactagaatc
      121 agtatttctt cttccatctc tgctgtacct gagaagaaat ggccaaacgc accttctcta
      181 acttggagac attcctgatt ttcctccttg taatgatgag tgccatcaca gtggcccttc
      241 tcagcctctt gtttatcacc agtgggacca ttgaaaacca caaagattta ggaggccatt
      301 ttttttcaac cacccaaagc cctccagcca cccagggctc cacagctgcc caacgctcca
      361 cagccaccca gcattccaca gccacccaga gctccacagc cactcaaact tctccagtgc
      421 ctttaacccc agagtctcct ctatttcaga acttcagtgg ctaccatatt ggtgttggac
      481 gagctgactg cacaggacaa gtagcagata tcaatttgat gggctatggc aaatccggcc
      541 agaatgcaca gggcatcctc accaggctat acagtcgtgc cttcatcatg gcagaacctg
      601 atgggtccaa tcgaacagtg tttgtcagca tcgacatagg catggtatca caaaggctca
      661 ggctggaggt cctgaacaga ctgcagagta aatatggctc cctgtacaga agagataatg
      721 tcatcctgag tggcactcac actcattcag gtcctgcagg atatttccag tataccgtgt
      781 ttgtaattgc cagtgaagga tttagcaatc aaacttttca gcacatggtc actggtatct
      841 tgaagagcat tgacatagca cacacaaata tgaaaccagg caaaatcttc atcaataaag
      901 gaaatgtgga tggtgtgcag atcaacagaa gtccgtattc ttaccttcaa aatccgcagt
      961 cagagagagc aaggtattct tcaaatacag acaaggaaat gatagttttg aaaatggtag
     1021 atttgaatgg agatgacttg ggccttatca gctggtttgc catccacccg gtcagcatga
     1081 acaacagtaa ccatcttgta aacagtgaca atgtgggcta tgcatcttac ctgcttgagc
     1141 aagagaagaa caaaggatat ctacctggac aggggccatt tgtagcagcc tttgcttcat
     1201 caaacctagg agatgtgtcc cccaacattc ttggaccacg ttgcatcaac acaggagagt
     1261 cctgtgataa cgccaatagc acttgtccca ttggtgggcc tagcatgtgc attgctaagg
     1321 gacctggaca ggatatgttt gacagcacac aaattatagg acgggccatg tatcagagag
     1381 caaaggaact ctatgcctct gcctcccagg aggtaacagg accactggct tcagcacacc
     1441 agtgggtgga tatgacagat gtgactgtct ggctcaattc cacacatgca tcaaaaacat
     1501 gtaaaccagc attgggctac agttttgcag ctggcactat tgatggagtt ggaggcctca
     1561 attttacaca ggggaaaaca gaaggggatc cattttggga caccattcgg gaccagatcc
     1621 tgggaaagcc atctgaagaa attaaagaat gtcataaacc aaagcccatc cttcttcaca
     1681 ccggagaact atcaaaacct cacccctggc atccagacat tgttgatgtt cagattatta
     1741 cccttgggtc cttggccata actgccatcc ccggggagtt tacgaccatg tctggacgaa
     1801 gacttcgaga ggcagttcaa gcagaatttg catctcatgg gatgcagaac atgactgttg
     1861 ttatttcagg tctatgcaac gtctatacac attacattac cacttatgaa gaataccagg
     1921 ctcagcgata tgaggcagca tcgacaattt atggaccgca cacattatct gcttacattc
     1981 agctcttcag aaaccttgct aaggctattg ctacggacac ggtagccaac ctgagcagag
     2041 gtccagaacc tccctttttc aaacaattaa tagttccatt aattcctagt attgtggata
     2101 gagcaccaaa aggcagaact ttcggggatg tcctgcagcc agcaaaacct gaatacagag
     2161 tgggggaagt tgctgaagtt atatttgtag gtgctaaccc gaagaattca gtacaaaacc
     2221 agacccatca gaccttcctc actgtggaga aatatgaggc tacttcaaca tcgtggcaga
     2281 tagtgtgtaa tgatgcctcc tgggagactc gtttttattg gcacaaggga ctcctgggtc
     2341 tgagtaatgc aacagtggaa tggcatattc cagacactgc ccagcctgga atctacagaa
     2401 taagatattt tggacacaat cggaagcagg acattctgaa gcctgctgtc atactttcat
     2461 ttgaaggcac ttccccggct tttgaagttg taactattta gtgaaaagtt gatagatcat
     2521 ttaaagaaca gctttactct ctacacatta tataagtgat ttcaaatgaa tgtgaactag
     2581 tgaactacca tgttgacttc tataatcgtc cctgtttggg gacagatagt ttactgctaa
     2641 tggggtggag gggtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgaga
     2701 gagagagaga gagagagaga gagagagaga ggtttgtccc atatatcttg ctccagcagc
     2761 catatatctt gtggtctaca gcctaaagca tgatttccct tgaagtcttg gggttgttta
     2821 aaggagagtc ccttcaatat aaaacctctg aaatattagt gagaatggct cactaatgtg
     2881 aacaatgttt aaattattta tttatatata gaattactga atattagtac tgggaaaatt
     2941 tatagaaatc atctagtctt acccttcatc ttacatataa gaaaaatggt cttttcttct
     3001 aatcacattt acaaaatatg atataaacct tgaccatgaa tgtatgagcc taattagaga
     3061 aacagaaaat cagcatgtca gttttccttc attcaaaata acatagtctt tctaagcagt
     3121 cattctggag ttaacatgcc tagttcagag ccgctgtgga tgctcaaatc atttctggaa
     3181 ttgccttcag gacacaatta tgagaaagtc agacttccaa tattttgatc atgccttgtg
     3241 ttttcctaag tgtccttatc ccactggatt gtgtctctta ctcccaagac ttattccaaa
     3301 tgattttttg actcttttca ttaatcaaat tcaccccaaa ccagaaagtt ttgccattag
     3361 tgtagatatt aaaaatatta ctggctggga aagcactcct caaagcagag gttctaaatt
     3421 aatttatctg ttcttttacc atttgctcac tcactcactc atggacccat tcaatcatag
     3481 aatatttaag atttatatgc ccagaattat aatagtgcct aggccttgat aattagtgaa
     3541 tttgtacttg gggtgaatgt gtaagtgtca aaaggtaact attatgaagg agaaaataat
     3601 cgatttaata tgtaaattct ggggaagcat ttaaaaaatc agtaatcttc aagttatatc
     3661 ttgtgtatca tctgtgttgg atactctaat ttggggggaa agaggctcca gacagtttgc
     3721 taaatactat tttgaaagtt atttttgggc attgtaaaat accttgcttc ataccccaaa
     3781 gaataagata tgacagtctg acatcgattg tctgaaagta ctaattgcat ttaaaattct
     3841 ttaagttaac tacaacctcc cagactgcaa agcaaatttc caaaccccag gtcatacttt
     3901 tgtatgtatg taaaagcttt agcattttag ggaaaaaaat caatctgtat tttaaaaata
     3961 aactttgagg tttgaaaata ataacaatat tcataatggt agtattaaca tctattatgt
     4021 ataacttaat ctcaacataa tttattgaat tagaatgaac ctggaacact ttttgatgaa
     4081 ttggtatcct agaggctgtt tccttgggca ttaactgctg gaggattagg taatcaggga
     4141 ttccaagtgt tgtgatcttt tttctgatga tgccacagtc ttcagtgtaa aagttcagaa
     4201 gcctaaaaga acaagtgatc ctgaaagtag ctgattccag acaagaaaat tatattagaa
     4261 cttatgatat ggttttttaa agtatttgtt attactcaga agggttcatg aatcagtttc
     4321 cactacctct attccttcta ttgaagcttt cctcaaatct acttgtgatt cctgatagct
     4381 cactggcaac tgtaccaagg tggccactgt atcatctact gatcctactg cacctagcac
     4441 tcttggctcc agataacgca cagaaaccta ttttctcacc agctgtcatt tcttctaggg
     4501 catgcagttc tatgttcgca gttcatgtgt aaaaataaaa ccaaattcct ggattatcaa
     4561 tatcagttct ttacctgtgc ataaaagaat atcatgaagt aattgaactt atctgttgat
     4621 ataaaaacac agatccaatt catgtctgtg gatgctgcag ggactggctc actggagcca
     4681 aaccatgtgg cagaaactcc tgaaattggt ggaaaaagtg gaatatggca gattgggtag
     4741 gtaggagaac aattttggag attttgtttc tcatgttaaa atgtctgcat tttatttgct
     4801 gtaccctgcc tactctggtt ttaaggattt tttgacttct ttgaaaagca ctgaataatc
     4861 tcttttccag gtctagattt tttgatgagt actgtgtaaa attgtcaata ttcaatggat
     4921 tatttaataa taaattaaga attacactca taaaa
//
