LOCUS       NM_004041               7352 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens arrestin beta 1 (ARRB1), transcript variant 1, mRNA.
ACCESSION   NM_004041
VERSION     NM_004041.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Kim,K., Ashim,J., Ham,D., Yu,W. and Chung,K.Y.
  TITLE     Roles of the gate loop in beta-arrestin-1 conformational dynamics
            and phosphorylated receptor interaction
  JOURNAL   Structure 32 (9), 1358-1366 (2024)
   PUBMED   38889722
  REMARK    GeneRIF: Roles of the gate loop in beta-arrestin-1 conformational
            dynamics and phosphorylated receptor interaction.
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   Del Rio,D., Masi,I., Caprara,V., Ottavi,F., Albertini Petroni,G.,
            Salvati,E., Trisciuoglio,D., Giannitelli,S.M., Bagnato,A.,
            Mauri,E., Spadaro,F. and Rosano,L.
  TITLE     The beta-arrestin1/endothelin axis bolsters ovarian
            fibroblast-dependent invadosome activity and cancer cell metastatic
            potential
  JOURNAL   Cell Death Dis 15 (5), 358 (2024)
   PUBMED   38777849
  REMARK    GeneRIF: The beta-arrestin1/endothelin axis bolsters ovarian
            fibroblast-dependent invadosome activity and cancer cell metastatic
            potential.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7352)
  AUTHORS   Nayyar,S., Archibong,A. and Nayyar,T.
  TITLE     Testosterone and Prolactin Perturbations Possibly Associated with
            Reduced Levels of beta-Arrestin1 in Mononuclear Leukocytes of Women
            with Premenstrual Dysphoric Disorder
  JOURNAL   Int J Mol Sci 24 (20), 15449 (2023)
   PUBMED   37895130
  REMARK    GeneRIF: Testosterone and Prolactin Perturbations Possibly
            Associated with Reduced Levels of beta-Arrestin1 in Mononuclear
            Leukocytes of Women with Premenstrual Dysphoric Disorder.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7352)
  AUTHORS   Manning,J.J., Rawcliffe,G., Finlay,D.B. and Glass,M.
  TITLE     Cannabinoid 1 (CB1) receptor arrestin subtype-selectivity and
            phosphorylation dependence
  JOURNAL   Br J Pharmacol 180 (3), 369-382 (2023)
   PUBMED   36250246
  REMARK    GeneRIF: Cannabinoid 1 (CB1) receptor arrestin subtype-selectivity
            and phosphorylation dependence.
REFERENCE   5  (bases 1 to 7352)
  AUTHORS   Masi,I., Ottavi,F., Del Rio,D., Caprara,V., Vastarelli,C.,
            Giannitelli,S.M., Fianco,G., Mozetic,P., Buttarelli,M.,
            Ferrandina,G., Scambia,G., Gallo,D., Rainer,A., Bagnato,A.,
            Spadaro,F. and Rosano,L.
  TITLE     The interaction of beta-arrestin1 with talin1 driven by endothelin
            A receptor as a feature of alpha5beta1 integrin activation in
            high-grade serous ovarian cancer
  JOURNAL   Cell Death Dis 14 (1), 73 (2023)
   PUBMED   36717550
  REMARK    GeneRIF: The interaction of beta-arrestin1 with talin1 driven by
            endothelin A receptor as a feature of alpha5beta1 integrin
            activation in high-grade serous ovarian cancer.
            Erratum:[Cell Death Dis. 2024 Oct 21;15(10):766. doi:
            10.1038/s41419-024-07139-x.. PMID: 39433739]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7352)
  AUTHORS   Goodman,O.B. Jr., Krupnick,J.G., Gurevich,V.V., Benovic,J.L. and
            Keen,J.H.
  TITLE     Arrestin/clathrin interaction. Localization of the arrestin binding
            locus to the clathrin terminal domain
  JOURNAL   J Biol Chem 272 (23), 15017-15022 (1997)
   PUBMED   9169477
REFERENCE   7  (bases 1 to 7352)
  AUTHORS   Iacovelli,L., Franchetti,R., Masini,M. and De Blasi,A.
  TITLE     GRK2 and beta-arrestin 1 as negative regulators of thyrotropin
            receptor-stimulated response
  JOURNAL   Mol Endocrinol 10 (9), 1138-1146 (1996)
   PUBMED   8885248
REFERENCE   8  (bases 1 to 7352)
  AUTHORS   Calabrese,G., Sallese,M., Stornaiuolo,A., Morizio,E., Palka,G. and
            De Blasi,A.
  TITLE     Assignment of the beta-arrestin 1 gene (ARRB1) to human chromosome
            11q13
  JOURNAL   Genomics 24 (1), 169-171 (1994)
   PUBMED   7896272
REFERENCE   9  (bases 1 to 7352)
  AUTHORS   Parruti,G., Peracchia,F., Sallese,M., Ambrosini,G., Masini,M.,
            Rotilio,D. and De Blasi,A.
  TITLE     Molecular analysis of human beta-arrestin-1: cloning, tissue
            distribution, and regulation of expression. Identification of two
            isoforms generated by alternative splicing
  JOURNAL   J Biol Chem 268 (13), 9753-9761 (1993)
   PUBMED   8486659
REFERENCE   10 (bases 1 to 7352)
  AUTHORS   Lohse,M.J., Benovic,J.L., Codina,J., Caron,M.G. and Lefkowitz,R.J.
  TITLE     beta-Arrestin: a protein that regulates beta-adrenergic receptor
            function
  JOURNAL   Science 248 (4962), 1547-1550 (1990)
   PUBMED   2163110
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC003636.2, AP001972.4,
            AK293758.1, DA358665.1, AL157484.1 and BM681392.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_004041.4.
            
            Summary: Members of arrestin/beta-arrestin protein family are
            thought to participate in agonist-mediated desensitization of
            G-protein-coupled receptors and cause specific dampening of
            cellular responses to stimuli such as hormones, neurotransmitters,
            or sensory signals. Arrestin beta 1 is a cytosolic protein and acts
            as a cofactor in the beta-adrenergic receptor kinase (BARK)
            mediated desensitization of beta-adrenergic receptors. Besides the
            central nervous system, it is expressed at high levels in
            peripheral blood leukocytes, and thus the BARK/beta-arrestin system
            is believed to play a major role in regulating receptor-mediated
            immune functions. Alternatively spliced transcripts encoding
            different isoforms of arrestin beta 1 have been described.
            [provided by RefSeq, Jan 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript variant and encodes the longer isoform (A).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.2651536.1,
                                           SRR11853558.4522.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000420843.7/ ENSP00000409581.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1925              BC003636.2         24-1948
            1926-3319           AP001972.4         167564-168957       c
            3320-4862           AK293758.1         18-1560
            4863-5007           DA358665.1         35-179
            5008-6290           AL157484.1         1-1283
            6291-6292           AP001972.4         164591-164592       c
            6293-7345           AL157484.1         1284-2336
            7346-7352           BM681392.1         18-24               c
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.4"
     gene            1..7352
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /note="arrestin beta 1"
                     /db_xref="GeneID:408"
                     /db_xref="HGNC:HGNC:711"
                     /db_xref="MIM:107940"
     exon            1..74
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..1311
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /note="isoform A is encoded by transcript variant 1;
                     arrestin 2; non-visual arrestin-2"
                     /codon_start=1
                     /product="beta-arrestin-1 isoform A"
                     /protein_id="NP_004032.2"
                     /db_xref="CCDS:CCDS44684.1"
                     /db_xref="GeneID:408"
                     /db_xref="HGNC:HGNC:711"
                     /db_xref="MIM:107940"
                     /translation="MGDKGTRVFKKASPNGKLTVYLGKRDFVDHIDLVDPVDGVVLVD
                     PEYLKERRVYVTLTCAFRYGREDLDVLGLTFRKDLFVANVQSFPPAPEDKKPLTRLQE
                     RLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGKACGVDYEVKAFCAENLEEKIHK
                     RNSVRLVIRKVQYAPERPGPQPTAETTRQFLMSDKPLHLEASLDKEIYYHGEPISVNV
                     HVTNNTNKTVKKIKISVRQYADICLFNTAQYKCPVAMEEADDTVAPSSTFCKVYTLTP
                     FLANNREKRGLALDGKLKHEDTNLASSTLLREGANREILGIIVSYKVKVKLVVSRGGL
                     LGDLASSDVAVELPFTLMHPKPKEEPPHREVPENETPVDTNLIELDTNDDDIVFEDFA
                     RQRLKGMKDDKEEEEDGTGSPQLNNR"
     exon            75..105
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     exon            106..166
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     exon            167..211
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     exon            212..408
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     exon            409..468
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     exon            469..536
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     exon            537..672
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     exon            673..757
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     exon            758..830
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     exon            831..968
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1052
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1076
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1147
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1199
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..7352
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3076..3081
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /note="hexamer: AATATA"
     polyA_site      3094
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
     regulatory      7313..7318
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /note="hexamer: AATAAA"
     polyA_site      7352
                     /gene="ARRB1"
                     /gene_synonym="ARB1; ARR1"
                     /note="major polyA site"
ORIGIN      
        1 gattttccag cctgggcgct gacgccgcgg acctccctgc gaccgtcgcg gaccatgggc
       61 gacaaaggga cccgagtgtt caagaaggcc agtccaaatg gaaagctcac cgtctacctg
      121 ggaaagcggg actttgtgga ccacatcgac ctcgtggacc ctgtggatgg tgtggtcctg
      181 gtggatcctg agtatctcaa agagcggaga gtctatgtga cgctgacctg cgccttccgc
      241 tatggccggg aggacctgga tgtcctgggc ctgacctttc gcaaggacct gtttgtggcc
      301 aacgtacagt cgttcccacc ggcccccgag gacaagaagc ccctgacgcg gctgcaggaa
      361 cgcctcatca agaagctggg cgagcacgct taccctttca cctttgagat ccctccaaac
      421 cttccatgtt ctgtgacact gcagccgggg cccgaagaca cggggaaggc ttgcggtgtg
      481 gactatgaag tcaaagcctt ctgcgcggag aatttggagg agaagatcca caagcggaat
      541 tctgtgcgtc tggtcatccg gaaggttcag tatgccccag agaggcctgg cccccagccc
      601 acagccgaga ccaccaggca gttcctcatg tcggacaagc ccttgcacct agaagcctct
      661 ctggataagg agatctatta ccatggagaa cccatcagcg tcaacgtcca cgtcaccaac
      721 aacaccaaca agacggtgaa gaagatcaag atctcagtgc gccagtatgc agacatctgc
      781 cttttcaaca cagctcagta caagtgccct gttgccatgg aagaggctga tgacactgtg
      841 gcacccagct cgacgttctg caaggtctac acactgaccc ccttcctagc caataaccga
      901 gagaagcggg gcctcgcctt ggacgggaag ctcaagcacg aagacacgaa cttggcctct
      961 agcaccctgt tgagggaagg tgccaaccgt gagatcctgg ggatcattgt ttcctacaaa
     1021 gtgaaagtga agctggtggt gtctcggggc ggcctgttgg gagatcttgc atccagcgac
     1081 gtggccgtgg aactgccctt caccctaatg caccccaagc ccaaagagga acccccgcat
     1141 cgggaagttc cagagaacga gacgccagta gataccaatc tcatagaact tgacacaaat
     1201 gatgacgaca ttgtatttga ggactttgct cgccagagac tgaaaggcat gaaggatgac
     1261 aaggaggaag aggaggatgg taccggctct ccacagctca acaacagata gacgggccgg
     1321 ccctgcctcc acgtggctcc ggctccactc tcgtgcactc ggatgcttac tcgtcttctt
     1381 cctgttctgg tttcttttcc cctttgttct tccagtttct accagggggc cccgtgggct
     1441 tccagatcac ggtgatgaac ctctggcctc aggattggcc ccacatcacc acgccaacag
     1501 gaccacagcg cactggctcc accccatctc tgccatctcc actcccctcc ttttcatgct
     1561 gtctcccaga aaagctgcca gggctctggc cttggaattg gacttgagat ggggagcaga
     1621 caggggagga tggggaatgt gggacacggt gtggtgggca tgagggcttg gaggggtggg
     1681 gatgagggct caagacacga gagaagatgt ccacggtccc aggtggttaa caaagttctg
     1741 gcagctaaaa gatgaccgcg ttgaaggcca cctccttctg gctgggaggg gcagaactgt
     1801 ggacagattc tcaatgcctt tttgaagttc tgacccacca aagaccttct gccttcaccc
     1861 tcctccccac ctgatgtccc tctgtgtctg atagtgatgt tggtgaaagt tcgtagaccc
     1921 caggagtaga gaaaagcaac tggactgact ttcttaccag cagttaccta gactgaggca
     1981 agctgtgtgg actcacccaa gtatatttca gtactgtcag gctgtgacat cttagctacc
     2041 ttgtatttca agtgcatggc cctgtctaga gatatgcaca gagagaaaag cagagggagg
     2101 gagggagggg gtcttcagca agcaccagcc cccagcagag tgagctgaag ctcctgagga
     2161 gggttcccga aggggggcgc tcagagatgg gggcaggggg cggggagagg agagtctgcc
     2221 ttatgtccct tccttgtgga cttcacatgg tcatgcagga agtgaggatg ggtgtccagc
     2281 gggggccgag gccactagta tcctcctgct tccccctgcc attctccagg gctggactga
     2341 ccctatggac tgggagagag tgcctgaggc caccatgcca cagtcaaagg gggtcctatc
     2401 tcagaaggtg gcagcatcca ctgagatatc ctcacccgaa gggaaggagg ctgctgggta
     2461 gcaaataagc cccttctttt cttggtgagt tgatgacctc caatagctcc cagtgtcatg
     2521 ggtacccagt acgcattagc tggtgttggg ttgattgaga cctggggcag ttcctggggc
     2581 aagaagccag atgggagatg agatagaaag tgttaggagt tatcctcttt gcctggcctt
     2641 tgagaataac ttactgtgtg actttgggca agttccttcc ccactctggg cctcagtttc
     2701 tcacttggga aagcaaggag tttgaccaga tgatcacaat gggccttcct agctctggcc
     2761 accaagaatt tgtgaacatt agagctcctg gtctggtggg tagagccaga gctgctgact
     2821 ggtctctctg cctccagagg ggatttattg gacctcagag gtggcagggc cctatggagc
     2881 accaactgcc ctcaacccca ccctgtgccc aagactggga agggattgat gtcaggctgt
     2941 ggccataggt agcatgagtt gcccaaggag ggacagagca tatctttgct gaggcttggc
     3001 tgaggggctt atgatagggc ttgcagtacc tcacagcccc ctgtgggcac agacaccctg
     3061 aggtttaccc aggcaaatat attgattagc aggacaaggg ctctctcctc agtttctgct
     3121 cccttccatc tctctcccat acttgtctga aaagggagac aaaaaatctt atacaagtgg
     3181 catctcaatc ctttccagtc cagcacctcc tggggcaggc agtgtgactt atttcctgtg
     3241 gtgaaatcac ctgtttcaca agcctggcag cgcgacttct gagtctcatg accactcaac
     3301 ccaagggacc cctccccaga ccagaaccaa gtcagctggg ggctgtcgta ctaccctgtc
     3361 cagtcttgag ggcctagttg caggtccccc aggcatccag cccctcctag agcttgctgg
     3421 gcaggctgca cctcatctgg gcaggcgcag agctgatgaa atgctggagc aatgcatggc
     3481 aaacatatgc cctccagtgt cttctgaaac ctttggggct gacacaagat cctttagtgt
     3541 ttgggatgac ctctttcctg cagacttctt cccctatccc taactcatgc atggaaaacg
     3601 tttgtcaggc tggtttcccg agcctcctgc acctcaacat cacgctcacc cttttgggtt
     3661 tagcccagtg ttatttagca aatttctcca gctgcaggga aggatcagag cactatcttt
     3721 tttttttttt tttctcctgg agccaggact gcacaaggca atggccaaat ttagttgaat
     3781 tcagcctacc atcctttgct gatgactcag ctctatgcca agtactggag ccacagagat
     3841 gggtcagtcc cagcccctgt cctcaggaag cccatggtca gggaaacgtt gtagggataa
     3901 gtaatagagg gcagttgcct tcagggctcc tggtggctgc tggtccctat ggtgccttga
     3961 tgtgaattag aagacggtgc cctttccagg tggattcaga cctacactag aacgcacagc
     4021 tttgggagtg acacacaggt tggattttag caccccttgc cccttggcca gaggtgccct
     4081 gctgcacggc catacgctgc agcctcgagg gacacacagg ccaaagtgtt tccttcagcc
     4141 tcttcctgga gaggaagccg caggtcatgt ttccaagctt ctggtcaact ccaggcacca
     4201 cctcccagcc cccaaacatg gccgagccag tgcttctctg cagggcccct ttcctttctg
     4261 gagtcagcct gcccaggtga taccttgaag tgaaactagg ggtgggggag cctcactagg
     4321 ccccaggcac catcccacag tactggcggt ttctctgcca agtgtttggg gcctgcactg
     4381 gccaagccat ccgtcactgg gactttgcag tgtgccctgt gggtggcgtg tggacaccgg
     4441 tccgggcagg aaggaaaagc atgccccggt gaaaacccac cgagcagaga gccagcggga
     4501 cctcctcagc attccaccca ggggaagagt tcagtcccca tgaagataag gactgagtta
     4561 tttggctgca cctctcctca gcctcaaagc tcagacctgc tagagtgggt tcacctggct
     4621 gttggttctg tgggagagtt cctgacaagc tggttcctac atggtgcagt gaaaggaact
     4681 ctgtcctgga agtccccaat gccgcctctc agtcctgcct gtcaccaacc acctgggggg
     4741 cttgggcaag ctactcaacc tctggggctc aatttcccta actgtaaaat gggattgtaa
     4801 tcccttccct gcctgacgca ggaattgtta tgaggcccag atgagatgaa agtcagcggt
     4861 cagggctctg taagttgaaa atggctggat gtgtgtggcc ctgccactgc agggatggcc
     4921 caaggtcaca cagaaagttt gctgcaggag gagccctccc caccagtctt catcgcttgc
     4981 cctgcttctc acttaggcag atgtcaacca gaatgagata cagccaattt ggctttgatg
     5041 acccagctga gagggaaatt tagatacaat tcaggaatat catgcccaaa aatagtggtc
     5101 ccagcaacca ggacacagag ggaagacctg gatattgtca gtgattggtg gagggggaca
     5161 tcttggaccc tcctaggcct ggtgtcacat ctcccatctc tcagacccca cagaggccaa
     5221 ggggccttgg ctctgaccca ggttaaagag agccaccagc ccccaggctc agggagctga
     5281 aaccacccat ccgcagaaat ctaagggcaa ggagaaatag aaattgtctg ttccttccct
     5341 tcaaagtgta aggcctcttc caggagcaac tggagccacc acagcttctc agtgacattg
     5401 ggagggcttc gggcccagga aaaacccaag ccttttgtcc tgagagccga gccacagcgg
     5461 atgcagtggc gtcagcatcc cagagcggca gcttctctcc tgcctgtcac tggggactgg
     5521 ttgctctcca cccatccagg aggaaggggt taggtcccag tcttacactc ttatagggtc
     5581 tcatggacat aggcctatgc ctgttctgat atctataatt ttttaaactc aaaaggacca
     5641 aaacactcct gaaccctcat ctatacggcc tgcactgggc ttggtgtttc ctcctggccc
     5701 aggcagctcc atctcttctg ccattcctcc tggacgaaag tggcaggacc ccacctctac
     5761 cacttcccca cagggtcact gcacagcggc ttcaaagaca cgccctgttg aaggggaaac
     5821 atcctccccc catcacactc caactcgtca ccccaaaagg gaagtcaaag cacaggtccg
     5881 tggggcctat ggtttgtaga cactgacaca gtggttttca aaccaggtgc tgcggaaccc
     5941 ctgatattcc acagagcttt gtcaggggat gctaaagtgg gaagatggga gtggaggcaa
     6001 aggataggtt tccagccctc ctcccatttc cgccgccacc agaataactc tgcttttatc
     6061 tgtcttgcct actgagattt cattttcaag ctttctttga agaagaactt caaaccacca
     6121 tctcgaggtt tgatcttaga ccagccagca caggttctat tcttgctgtt catgttggaa
     6181 tggctccagc gttccagtac aaagtgtctt tgtgttggaa cttgggtcac agacccaggc
     6241 acaagcattt atacacacac acacacacac acacacacac acacacacac acgtacatag
     6301 ttgatggttt ttctagccaa cccaccctga agctctggcc aaggtttgat ttggacaggg
     6361 acctggtgct cagtggggat gggtcgtgac cctgatattg gcccatgaag catggggagc
     6421 tctggagcaa ggaaacaggc tcaatccccc actgaggact tgtgtctgca cccctatctg
     6481 gcaccccact ttcctggcct aggcccaagg gacacagcag gagaagcagt gtgataggag
     6541 agaggctagg gttaaattca tctttacaca gcgccggatg gatggaaatc ttggctcaca
     6601 taaaagacag actcagggag gattttatga caaaagtccc tcctttgagg gcaggattcc
     6661 cccaggaatc ctggacactt tagtgagaac tggaccagcc cttggcacaa aaccctgcct
     6721 gccctgtccc ccactcccac cccccaggga attctaggtg aaaatgtgaa gctcagggca
     6781 cagcaagcgc atgacacggg tgtattacat tcggtccgag tgtgtcctta gtttccctgt
     6841 gtgcaaaatg gggctagtcc aggctcttcc cacctcactg ggacaattgc aagggtcata
     6901 ggagaggtgg gtatgatagg tggtcatatg aggtggtgag tatgagaggt gggtgcctca
     6961 caaactcggg gtatggtcct gacctgcatg gcagatgagc agagcacttg ggaacgagca
     7021 taggaagtgc catcttcccg ggcatctctg ctcagtgtta aggtctagcc acagacactg
     7081 tgggacctgc ctcttcctat ggaatggggg cctgcatggg ttgtgtcctg gggcaggagt
     7141 gagggatggc tgggaagaat ctccaggtct gtggcccttg tgttgagtga ttattttgtg
     7201 acactctcat tcctggacca cctgccaggt ttatctgtta tacctgtggc attcttatta
     7261 tgtcagacaa gttattatag ttcattcaac tctaacaaat gaacaaaaga acaataaata
     7321 ggaggccatt aaaggtcttt tgtggcagag ga
//
