LOCUS       NM_001385562            4158 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens cAMP regulated phosphoprotein 21 (ARPP21), transcript
            variant 28, mRNA.
ACCESSION   NM_001385562 XM_017005576
VERSION     NM_001385562.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Chan Moi Fat,S., McCann,E.P., Williams,K.L., Henden,L., Twine,N.A.,
            Bauer,D.C., Pamphlett,R., Kiernan,M.C., Rowe,D.B., Nicholson,G.A.,
            Fifita,J.A. and Blair,I.P.
  TITLE     Genetic analysis of GLT8D1 and ARPP21 in Australian familial and
            sporadic amyotrophic lateral sclerosis
  JOURNAL   Neurobiol Aging 101, 297 (2021)
   PUBMED   33581934
  REMARK    GeneRIF: Genetic analysis of GLT8D1 and ARPP21 in Australian
            familial and sporadic amyotrophic lateral sclerosis.
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 4158)
  AUTHORS   Li,W., Liu,Z., Sun,W., Yuan,Y., Hu,Y., Ni,J., Jiao,B., Fang,L.,
            Li,J., Shen,L., Tang,B. and Wang,J.
  TITLE     Mutation analysis of GLT8D1 and ARPP21 genes in amyotrophic lateral
            sclerosis patients from mainland China
  JOURNAL   Neurobiol Aging 85, 156 (2020)
   PUBMED   31653410
  REMARK    GeneRIF: Mutation analysis of GLT8D1 and ARPP21 genes in
            amyotrophic lateral sclerosis patients from mainland China.
REFERENCE   4  (bases 1 to 4158)
  AUTHORS   Marangi,G., Orteschi,D., Milano,V., Mancano,G. and Zollino,M.
  TITLE     Interstitial deletion of 3p22.3p22.2 encompassing ARPP21 and CLASP2
            is a potential pathogenic factor for a syndromic form of
            intellectual disability: a co-morbidity model with additional copy
            number variations in a large family
  JOURNAL   Am J Med Genet A 161A (11), 2890-2893 (2013)
   PUBMED   24127197
  REMARK    GeneRIF: Interstitial deletion of 3p22.3p22.2 encompassing ARPP21
            and CLASP2 is a potential pathogenic factor for a syndromic form of
            intellectual disability.
REFERENCE   5  (bases 1 to 4158)
  AUTHORS   Need,A.C., Attix,D.K., McEvoy,J.M., Cirulli,E.T., Linney,K.L.,
            Hunt,P., Ge,D., Heinzen,E.L., Maia,J.M., Shianna,K.V., Weale,M.E.,
            Cherkas,L.F., Clement,G., Spector,T.D., Gibson,G. and
            Goldstein,D.B.
  TITLE     A genome-wide study of common SNPs and CNVs in cognitive
            performance in the CANTAB
  JOURNAL   Hum Mol Genet 18 (23), 4650-4661 (2009)
   PUBMED   19734545
REFERENCE   6  (bases 1 to 4158)
  AUTHORS   Rakhilin,S.V., Olson,P.A., Nishi,A., Starkova,N.N., Fienberg,A.A.,
            Nairn,A.C., Surmeier,D.J. and Greengard,P.
  TITLE     A network of control mediated by regulator of
            calcium/calmodulin-dependent signaling
  JOURNAL   Science 306 (5696), 698-701 (2004)
   PUBMED   15499021
REFERENCE   7  (bases 1 to 4158)
  AUTHORS   Brill,L.M., Salomon,A.R., Ficarro,S.B., Mukherji,M.,
            Stettler-Gill,M. and Peters,E.C.
  TITLE     Robust phosphoproteomic profiling of tyrosine phosphorylation sites
            from human T cells using immobilized metal affinity chromatography
            and tandem mass spectrometry
  JOURNAL   Anal Chem 76 (10), 2763-2772 (2004)
   PUBMED   15144186
REFERENCE   8  (bases 1 to 4158)
  AUTHORS   Hu,R.M., Han,Z.G., Song,H.D., Peng,Y.D., Huang,Q.H., Ren,S.X.,
            Gu,Y.J., Huang,C.H., Li,Y.B., Jiang,C.L., Fu,G., Zhang,Q.H.,
            Gu,B.W., Dai,M., Mao,Y.F., Gao,G.F., Rong,R., Ye,M., Zhou,J.,
            Xu,S.H., Gu,J., Shi,J.X., Jin,W.R., Zhang,C.K., Wu,T.M.,
            Huang,G.Y., Chen,Z., Chen,M.D. and Chen,J.L.
  TITLE     Gene expression profiling in the human
            hypothalamus-pituitary-adrenal axis and full-length cDNA cloning
  JOURNAL   Proc Natl Acad Sci U S A 97 (17), 9543-9548 (2000)
   PUBMED   10931946
REFERENCE   9  (bases 1 to 4158)
  AUTHORS   Caporaso,G.L., Bibb,J.A., Snyder,G.L., Valle,C., Rakhilin,S.,
            Fienberg,A.A., Hemmings,H.C., Nairn,A.C. and Greengard,P.
  TITLE     Drugs of abuse modulate the phosphorylation of ARPP-21, a cyclic
            AMP-regulated phosphoprotein enriched in the basal ganglia
  JOURNAL   Neuropharmacology 39 (9), 1637-1644 (2000)
   PUBMED   10854908
REFERENCE   10 (bases 1 to 4158)
  AUTHORS   Brene,S., Lindefors,N., Ehrlich,M., Taubes,T., Horiuchi,A.,
            Kopp,J., Hall,H., Sedvall,G., Greengard,P. and Persson,H.
  TITLE     Expression of mRNAs encoding ARPP-16/19, ARPP-21, and DARPP-32 in
            human brain tissue
  JOURNAL   J Neurosci 14 (3 Pt 1), 985-998 (1994)
   PUBMED   8120638
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC016943.7 and AC104308.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jul 20, 2020 this sequence version replaced XM_017005576.2.
            
            Summary: This gene encodes a cAMP-regulated phosphoprotein. The
            encoded protein is enriched in the caudate nucleus and cerebellar
            cortex. A similar protein in mouse may be involved in regulating
            the effects of dopamine in the basal ganglia. Alternate splicing
            results in multiple transcript variants. [provided by RefSeq, Jun
            2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.2064781.1,
                                           SRR14038191.2108708.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2154529
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000684406.1/ ENSP00000506922.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-874               AC016943.7         65878-66751
            875-1048            AC016943.7         106140-106313
            1049-1215           AC016943.7         108067-108233
            1216-1257           AC016943.7         109201-109242
            1258-1347           AC016943.7         110079-110168
            1348-1492           AC016943.7         114092-114236
            1493-1571           AC016943.7         115660-115738
            1572-1631           AC016943.7         116434-116493
            1632-1772           AC016943.7         117218-117358
            1773-1881           AC016943.7         133327-133435
            1882-1983           AC016943.7         135322-135423
            1984-2021           AC016943.7         141792-141829
            2022-2081           AC016943.7         143651-143710
            2082-2311           AC016943.7         147958-148187
            2312-2545           AC016943.7         155656-155889
            2546-2730           AC016943.7         163531-163715
            2731-2835           AC016943.7         164567-164671
            2836-3096           AC016943.7         165670-165930
            3097-3223           AC016943.7         170192-170318
            3224-3372           AC104308.3         38475-38623
            3373-4158           AC104308.3         39794-40579
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p22.3"
     gene            1..4158
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /note="cAMP regulated phosphoprotein 21"
                     /db_xref="GeneID:10777"
                     /db_xref="HGNC:HGNC:16968"
                     /db_xref="MIM:605488"
     exon            1..874
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            875..1048
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1215
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     CDS             1087..3630
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /note="isoform 6 is encoded by transcript variant 28;
                     cyclic AMP-regulated phosphoprotein, 21 kD; R3H domain
                     containing 3; thymocyte cAMP-regulated phosphoprotein;
                     cAMP regulated phosphoprotein 21kDa"
                     /codon_start=1
                     /product="cAMP-regulated phosphoprotein 21 isoform 6"
                     /protein_id="NP_001372491.1"
                     /db_xref="CCDS:CCDS93238.1"
                     /db_xref="GeneID:10777"
                     /db_xref="HGNC:HGNC:16968"
                     /db_xref="MIM:605488"
                     /translation="MSEQGDLNQAIAEEGGTEQETATPENGIVKSESLDEEEKLELQR
                     RLEAQNQERRKSKSGAGKGKLTRSLAVCEESSARPGGESLQDQESIHLQLSSFSSLQE
                     EDKSRKDDSEREKEKDKNKDKTSEKPKIRMLSKDCSQEYTDSTGIDLHEFLINTLKNN
                     SRDRMILLKMEQEIIDFIADNNNHYKKFPQMSSYQRMLVHRVAAYFGLDHNVDQTGKS
                     VIINKTSSTRIPEQRFCEHLKDEKGEESQKRFILKRDNSSIDKEDNQQNRMHPFRDDR
                     RSKSIEEREEEYQRVRERIFAHDSVCSQESLFVENSRLLEDSNICNETYKKRQLFRGN
                     RDGSGRTSGSRQSSSENELKWSDHQRAWSSTDSDSSNRNLKPAMTKTASFGGITVLTR
                     GDSTSSTRSTGKLSKAGSESSSSAGSSGSLSRTHPPLQSTPLVSGVAAGSPGCVPYPE
                     NGIGGQVAPSSTSYILLPLEAATGIPPGSILLNPHTGQPFVNPDGTPAIYNPPTSQQP
                     LRSAMVGQSQQQPPQQQPSPQPQQQVQPPQPQMAGPLVTQSVQGLQASSQSVQYPAVS
                     FPPQHLLPVSPTQHFPMRDDVATQFGQMTLSRQSSGETPEPPSGPVYPSSLMPQPAQQ
                     PSYVIASTGQQLPTGGFSGSGPPISQQVLQPPPSPQGFVQQPPPAQMPVYYYPSGQYP
                     TSTTQQYRPMAPVQYNAQRSQQMPQAAQQAGYQPVLSGQQGFQGLIGVQQPPQSQNVI
                     NNQQGTPVQSVMVSYPTMSSYQVPMTQGSQGLPQQSYQQPIMLPNQAGQGSLPATGMP
                     VYCNVTPPTPQNNLRLIGPHCPSSTVPVMSASCRTNCASMSNAGWQVKF"
     exon            1216..1257
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1258..1347
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1348..1492
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1571
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1631
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1772
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1773..1881
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1882..1983
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2021
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            2022..2081
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            2082..2311
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            2312..2545
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            2546..2730
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            2731..2835
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            2836..3096
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            3097..3223
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            3224..3372
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     exon            3373..4158
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4112..4117
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /note="hexamer: AATAAA"
     polyA_site      4158
                     /gene="ARPP21"
                     /gene_synonym="ARPP-21; R3HDM3; RCS; TARPP"
                     /note="major polyA site"
ORIGIN      
        1 agacgacgga ggcggccaag ctggaggaac gggaccgagg gatcgaccga ggctggctgg
       61 ctaggggtgg gactggcaga gctacggaca ggtgggagca gtcagacagc ctgagagagg
      121 gagaccagaa cgcaaagaga gcgagagcat taagtaggca gggagaggca cacagaaaga
      181 tagacagcaa cactcgcgtg ctccctccct ccacacgccc tcctcttacc gagcgaagcg
      241 gccaggggca cagagtggga gaggactgtg ggcacagaag accggaagag ggacgctgca
      301 ttgctggtgg gacggaggga ttcggacgga cggacagacc agcgcacaga ctggcagcct
      361 gcgagagtgg cagctttgag agcagagcgc agcaaagcga gaatcccgcg ccgagctgct
      421 agtcggaccc acagacggtc ggactgacag acgtgcagac ggacagaaga gcagacagga
      481 ggcgggggcg cgttcactcc cggcggccag ccggccagag aaggcggcgg tggcaccttc
      541 tgagcgcgtc cggagcagag accagcagca gcaggggcag cggggacaca agccgcgacc
      601 gagccgccgc cgcagccctg gctgccacag ctgtgtggga tcccagccga cactgcagaa
      661 agctcctttt actagcagtt aaataaatcg accaaaaaca aaacaaacaa acaaacccaa
      721 caacaacaac aacaacaaaa gccaagccaa aagccaaaac aaacaaatcc agccaaatca
      781 atcattgaga aacaataata ttaataaaat ccaaaactaa atcaaaccaa tcaaggcacc
      841 aagacgcagg ggggatatcc acagttcgat caaggttgac gatgtgtcac actgtgtaag
      901 ggaatcgcat ggagatgggc attccgaact gttaatgggg acatgggact ccagttgtct
      961 ctgatcactt gtgtggattt tcctggcgta gaacgacaga agccgctagt aagtcgccaa
     1021 gacctacagc aggaattctg caccaaaggg cataaaatct tgttatttta atttgcatct
     1081 gggagaatgt ctgagcaagg agacctgaat caggcaatag cagaggaagg agggactgag
     1141 caggagacgg ccactccaga gaacggcatt gttaaatcag aaagtctgga tgaagaggag
     1201 aaactggaac tgcagaggcg gctggaggct cagaatcaag aaagaagaaa atccaagtca
     1261 ggagcaggaa aaggtaaact gactcgcagc cttgctgtct gtgaggaatc ttctgccaga
     1321 ccaggaggtg aaagtcttca ggatcaggaa tcaattcatt tacagctttc cagtttttcc
     1381 agcctgcaag aggaggataa atctaggaaa gatgactctg aaagagaaaa agaaaaggat
     1441 aaaaacaaag ataaaacctc tgaaaaaccc aagatcagaa tgttatcaaa agattgcagc
     1501 caagaataca cggattctac aggcatagac ttacacgagt ttctgattaa cacattaaag
     1561 aataattcca gggacaggat gatacttttg aaaatggagc aggaaattat tgatttcatt
     1621 gctgacaaca ataatcatta taaaaagttc cctcagatgt catcgtatca gaggatgctt
     1681 gtccatcgag tggcagctta ttttggattg gatcacaatg tggatcaaac aggaaaatct
     1741 gttatcatca acaagaccag cagcaccaga ataccagagc aaaggttttg tgaacattta
     1801 aaagatgaaa aaggtgaaga atcccagaag cggtttatct tgaagcgaga taactctagt
     1861 attgataaag aagacaatca gcaaaacaga atgcatccat ttagagatga cagacgaagt
     1921 aaatcaattg aagagagaga agaggaatat cagagagtga gggagagaat atttgcacac
     1981 gattcagttt gctcccagga aagccttttt gtggaaaaca gtaggctctt ggaagacagt
     2041 aacatatgca atgagaccta taagaaaaga cagctctttc ggggcaacag agatggctca
     2101 gggagaacat ctgggagtcg acagagcagc tcagaaaatg aactcaagtg gtctgaccac
     2161 caaagggcct ggagcagcac agactccgac agttccaacc gcaatctaaa gcccgccatg
     2221 accaagacgg cgagttttgg gggcatcacg gtgctgacca ggggtgacag cacttccagt
     2281 actaggagta ccgggaagct gtccaaagca ggttccgagt cttccagcag tgcaggctcc
     2341 tcaggatcgc tgtcccgcac ccatccacct ctccagagca cacccctagt ctcaggtgtg
     2401 gcagctggct ctccaggctg tgtgccttat ccagagaatg gaataggggg ccaggttgct
     2461 cccagcagca ccagctacat cctccttcca cttgaagctg caacaggcat cccgcctgga
     2521 agcatccttc ttaatccaca cacaggccag ccctttgtga atcccgatgg aactcctgca
     2581 atatacaacc cacccaccag tcagcagccc ctgcgaagcg ccatggtggg gcagtcccaa
     2641 cagcagccac cacagcagca gccctccccg cagccccaac agcaggtcca gccaccgcag
     2701 ccacagatgg caggccctct ggtcactcag tctgtccagg ggctgcaggc ttcctcccag
     2761 tcagtgcaat atccagcagt ctcttttcct ccccagcacc tcctacctgt gtctccaacg
     2821 cagcactttc ccatgagaga tgatgtggca acacagtttg gccagatgac cctgagccgg
     2881 cagtcctcgg gggagactcc tgaaccccca tcaggtcctg tctacccatc ctcccttatg
     2941 ccacagccgg cccagcagcc cagctatgta atcgcctcta caggccagca gcttcctaca
     3001 ggaggattct caggctctgg ccctcccatc tcccagcagg tcctccagcc ccctccctca
     3061 ccacagggat ttgtgcaaca gcctccgcct gcacagatgc ctgtatatta ttacccatct
     3121 ggtcagtacc ctacctcaac cacgcaacag taccggccca tggccccggt tcagtacaac
     3181 gctcagagga gtcaacagat gccacaggca gcacagcaag caggttacca gccagtcttg
     3241 tctggtcaac agggattcca aggcctaata ggagtgcagc agccacctca gagtcagaac
     3301 gtgataaata accaacaagg aactccggtg caaagcgtga tggtttccta cccaacaatg
     3361 tcttcttatc aggtgccaat gacccagggt tctcaaggac tgccccagca gtcataccaa
     3421 cagccaatca tgctacctaa ccaggcaggt caagggtcac tcccagccac tggaatgcct
     3481 gtttactgta atgtcacacc gcccacccct cagaacaacc ttaggctgat tggcccacac
     3541 tgcccctcca gcactgtccc agtgatgtca gctagctgca gaacaaactg tgcaagtatg
     3601 agcaatgctg gttggcaggt caaattctga gagctctggc tgtggtacat ttcttcagat
     3661 atttctcatg gcctttgatg gaagaggaac aaggtgggaa aactggctga ggacttaagt
     3721 attcactcaa cactcaaatg attgctgctg gtattctgta aaaaataaac aaagactaat
     3781 atacacgtta gctggttaat ggtgcatatt tctgtcatgt ctgctaggta tgcctttata
     3841 gcttagctag tgacatgaat tcatcaaggt aagattttct cctaccactg aataccactg
     3901 tgtagattat aatatcccta atttggatta gttttgtact ttgtgttgag tttgtgatgc
     3961 taaaagtatt taaaaattat atactaaatc acattgtacc aaagctgtaa tggaaaagca
     4021 aagaagaatt gatgaattga aggaataatt tatatacatt atagagtttt cttttttaat
     4081 ggatatatac tgtattgtag tgtttaatca aaataaaact atttgacctt atggaggaag
     4141 gtcatgtttt taccacca
//
