LOCUS       NM_030978               2507 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens actin related protein 2/3 complex subunit 5 like
            (ARPC5L), mRNA.
ACCESSION   NM_030978 XM_005252250
VERSION     NM_030978.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Sindram,E., Caballero-Oteyza,A., Kogata,N., Chor Mei Huang,S.,
            Alizadeh,Z., Gamez-Diaz,L., Fazlollhi,M.R., Peng,X., Grimbacher,B.,
            Way,M. and Proietti,M.
  TITLE     ARPC5 deficiency leads to severe early-onset systemic inflammation
            and mortality
  JOURNAL   Dis Model Mech 16 (7) (2023)
   PUBMED   37382373
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2507)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2507)
  AUTHORS   Fasci,D., van Ingen,H., Scheltema,R.A. and Heck,A.J.R.
  TITLE     Histone Interaction Landscapes Visualized by Crosslinking Mass
            Spectrometry in Intact Cell Nuclei
  JOURNAL   Mol Cell Proteomics 17 (10), 2018-2033 (2018)
   PUBMED   30021884
REFERENCE   5  (bases 1 to 2507)
  AUTHORS   Abella,J.V., Galloni,C., Pernier,J., Barry,D.J., Kjaer,S.,
            Carlier,M.F. and Way,M.
  TITLE     Isoform diversity in the Arp2/3 complex determines actin filament
            dynamics
  JOURNAL   Nat Cell Biol 18 (1), 76-86 (2016)
   PUBMED   26655834
REFERENCE   6  (bases 1 to 2507)
  AUTHORS   Wang,J., Huo,K., Ma,L., Tang,L., Li,D., Huang,X., Yuan,Y., Li,C.,
            Wang,W., Guan,W., Chen,H., Jin,C., Wei,J., Zhang,W., Yang,Y.,
            Liu,Q., Zhou,Y., Zhang,C., Wu,Z., Xu,W., Zhang,Y., Liu,T., Yu,D.,
            Zhang,Y., Chen,L., Zhu,D., Zhong,X., Kang,L., Gan,X., Yu,X., Ma,Q.,
            Yan,J., Zhou,L., Liu,Z., Zhu,Y., Zhou,T., He,F. and Yang,X.
  TITLE     Toward an understanding of the protein interaction network of the
            human liver
  JOURNAL   Mol Syst Biol 7, 536 (2011)
   PUBMED   21988832
  REMARK    Erratum:[Mol Syst Biol. 2017 Dec 18;13(12):965. doi:
            10.15252/msb.20178107.. PMID: 29254952]
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2507)
  AUTHORS   Kuo,J.C., Han,X., Hsiao,C.T., Yates,J.R. 3rd and Waterman,C.M.
  TITLE     Analysis of the myosin-II-responsive focal adhesion proteome
            reveals a role for beta-Pix in negative regulation of focal
            adhesion maturation
  JOURNAL   Nat Cell Biol 13 (4), 383-393 (2011)
   PUBMED   21423176
REFERENCE   8  (bases 1 to 2507)
  AUTHORS   Buschow,S.I., van Balkom,B.W., Aalberts,M., Heck,A.J., Wauben,M.
            and Stoorvogel,W.
  TITLE     MHC class II-associated proteins in B-cell exosomes and potential
            functional implications for exosome biogenesis
  JOURNAL   Immunol Cell Biol 88 (8), 851-856 (2010)
   PUBMED   20458337
REFERENCE   9  (bases 1 to 2507)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   10 (bases 1 to 2507)
  AUTHORS   Millard,T.H., Behrendt,B., Launay,S., Futterer,K. and Machesky,L.M.
  TITLE     Identification and characterisation of a novel human isoform of
            Arp2/3 complex subunit p16-ARC/ARPC5
  JOURNAL   Cell Motil Cytoskeleton 54 (1), 81-90 (2003)
   PUBMED   12451597
  REMARK    Erratum:[Cell Motil Cytoskeleton. 2003 Mar;54(3):266]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK056174.1, AL354928.9 and AA731112.1.
            
            On Nov 22, 2018 this sequence version replaced NM_030978.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK056174.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142586, SAMEA2145544
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000353214.6/ ENSP00000345361.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-608               AK056174.1         1-608
            609-609             AL354928.9         128515-128515
            610-1414            AK056174.1         610-1414
            1415-1415           AL354928.9         133573-133573
            1416-2498           AK056174.1         1416-2498
            2499-2507           AA731112.1         13-21               c
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q33.3"
     gene            1..2507
                     /gene="ARPC5L"
                     /gene_synonym="ARC16-2"
                     /note="actin related protein 2/3 complex subunit 5 like"
                     /db_xref="GeneID:81873"
                     /db_xref="HGNC:HGNC:23366"
     exon            1..269
                     /gene="ARPC5L"
                     /gene_synonym="ARC16-2"
                     /inference="alignment:Splign:2.1.0"
     exon            270..389
                     /gene="ARPC5L"
                     /gene_synonym="ARC16-2"
                     /inference="alignment:Splign:2.1.0"
     exon            390..1401
                     /gene="ARPC5L"
                     /gene_synonym="ARC16-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             1253..1714
                     /gene="ARPC5L"
                     /gene_synonym="ARC16-2"
                     /note="arp2/3 complex 16 kDa subunit 2; epididymis
                     secretory sperm binding protein"
                     /codon_start=1
                     /product="actin-related protein 2/3 complex subunit 5-like
                     protein"
                     /protein_id="NP_112240.1"
                     /db_xref="CCDS:CCDS6859.1"
                     /db_xref="GeneID:81873"
                     /db_xref="HGNC:HGNC:23366"
                     /translation="MARNTLSSRFRRVDIDEFDENKFVDEQEEAAAAAAEPGPDPSEV
                     DGLLRQGDMLRAFHAALRNSPVNTKNQAVKERAQGVVLKVLTNFKSSEIEQAVQSLDR
                     NGVDLLMKYIYKGFEKPTENSSAVLLQWHEKALAVGGLGSIIRVLTARKTV"
     exon            1402..1474
                     /gene="ARPC5L"
                     /gene_synonym="ARC16-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1651
                     /gene="ARPC5L"
                     /gene_synonym="ARC16-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1652..2507
                     /gene="ARPC5L"
                     /gene_synonym="ARC16-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2162..2167
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARPC5L"
                     /gene_synonym="ARC16-2"
                     /note="hexamer: AATAAA"
     polyA_site      2189
                     /gene="ARPC5L"
                     /gene_synonym="ARC16-2"
                     /note="major polyA site"
     regulatory      2480..2485
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARPC5L"
                     /gene_synonym="ARC16-2"
                     /note="hexamer: AATAAA"
     polyA_site      2507
                     /gene="ARPC5L"
                     /gene_synonym="ARC16-2"
                     /note="major polyA site"
ORIGIN      
        1 attcacggca cccctgatag cgaggtcggc gtcacggtgt aggcgcgcag ttggggaaac
       61 cgaggcccaa cgtgggcggc agctaccaga gatggcacac ggaggactca aattcgtcca
      121 ttcagtcatt cgttcattca ttccttcggg aaaccttgca gatctcccaa tctgggcaac
      181 tctgagctgg gcgccagtag tctgggggtg ctgacgggag gaggggccag agttttcaaa
      241 gacgattaac cgaggctcac gtcgcggagc caacctagaa gttctctggg gacagaacct
      301 tggcggacaa ctgaattaat tgttctagtc tccaggccca gtacatggat gcttgttgga
      361 tgaattaatg aaccttgacc cacaccaaga ttctgagaat ttggtttctg tcaagatgtg
      421 cctctccagc caggcctctc ctgccaggac ggtggccaca gggccatctc cctctcctgg
      481 cgcccccaac tgcctggcca aggtgactgc tacacacgtg gggtcgcaga atccctcgag
      541 tcacaacgtc ttcaacgtct tgtcagtccc gtgatcgcac taaacgtatt taaagccccc
      601 tactccttta agagggtcac ctctgtgtac aggcggcctc ctcgtactaa gtgtggtggc
      661 gttcaaggct ccgagacaca ggcgcctccc gcgccactcc ccgggcacag gaactcctac
      721 tcatccctca gtcatcagct cgaaggtttc ttctccaaga agcctgcccg gcactcgaga
      781 agtagagccg cgagccttct cccgggggcc cagccttccc cagtaccggc agggatcacc
      841 ccgggagcac gcgcccggcc ccgctgaggc caggggctcc tcggaggcgc gggaaagccg
      901 gggaaccagc gcagttacgg cacacacgag gcggggcctg cacagatgcc gggcgcccga
      961 tcctcagtga caaaatagag actccgtgga agggacactg aggtggggga ggctgcggtg
     1021 atcccatacc gcactccagg tgccaggctc cgccccgccc ctgacggcgc ttccggatcc
     1081 ggcgggtgcc ggaagtgggc gggcggcggc ggctgcgcgc ggaggcggtg gaggaggtgc
     1141 tgggagcagc cgggcagccg cttcccgccc ccgagcagga gccggtgcga gcggagcaga
     1201 gccgaggtcg ggccgcgagc ggagccggct gagcgggcgc cgagctcccg ccatggcccg
     1261 gaacacgctg tcctcgcgct tccgccgggt ggacatcgac gaatttgacg agaacaaatt
     1321 tgtggacgag caggaggagg cggcggcggc ggcggcggag ccaggcccgg acccgagcga
     1381 ggtggacggg ctcctgcggc aaggggacat gcttcgggca ttccatgcag ccttgcggaa
     1441 ctctcccgtc aacaccaaga atcaagctgt gaaggagcga gcccagggcg tggtgctgaa
     1501 agtgctcaca aacttcaaga gcagtgagat tgagcaggct gtgcagtcac tggacagaaa
     1561 cggcgttgac ttgttaatga agtacattta taaaggcttt gagaagccca cagaaaatag
     1621 cagcgcagtg ttactccagt ggcacgaaaa ggccttagca gtaggaggac taggctccat
     1681 tataagagtt cttacagcaa gaaagactgt ttaaaaaaaa taaaaagact catgttacct
     1741 tgagaagaat tctggatgcc caggctggtg aagaagggat tgacaatgga ccatcttcct
     1801 aggaactccc aagtaaacta tttcaggaca tgtatctgct gaaatgtatt ttattttcaa
     1861 ggtggagggg aaaatcgtct gtttcctaaa tcctgtttag gatctgatag tctatgcctt
     1921 tgtctccgag tactgcagaa ctgacatttt gacggtctac cagcgtggcg gctggtgttg
     1981 gtcagatgca cctgtgtgca ctgggggagg gatggtttgg gcaggtgcag atccaagggc
     2041 tgtggtaaac gggagagctt gtgtttttga agtggaaaaa aacccaagag tttgtacaga
     2101 catcctgtct tcccagagaa ggtggacact cttgggctca ttgtaaagtg cctgctgcat
     2161 caataaagct cttggcttat tagtctatac attgcggtgt gtttcgtgta tgtaaaaaaa
     2221 aatggtaatg aatgggatgg taatgaatga gagttcagtt gttgttccgg aaacccgatg
     2281 tggaaggagt agacctgtgt ccctgttgag ccacccctgg gagcgagcat ggcaatccac
     2341 aggccctctg ccacaggacg ccagcctcgg cctcagagct gccggctgct gcagagaggt
     2401 gtttgctgaa taaactattt attgtttctt attcctttga tttgtatgta attaattttg
     2461 gagcttattt aattaattta ataaagtgcc aaacatttaa taattga
//
