LOCUS       NM_005718               1433 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens actin related protein 2/3 complex subunit 4 (ARPC4),
            transcript variant 1, mRNA.
ACCESSION   NM_005718
VERSION     NM_005718.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1433)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1433)
  AUTHORS   Xu,N., Qu,G.Y., Wu,Y.P., Lin,Y.Z., Chen,D.N., Li,X.D., Chen,S.H.,
            Huang,J.B., Zheng,Q.S., Xue,X.Y. and Wei,Y.
  TITLE     ARPC4 promotes bladder cancer cell invasion and is associated with
            lymph node metastasis
  JOURNAL   J Cell Biochem 121 (1), 231-243 (2020)
   PUBMED   31190401
  REMARK    GeneRIF: ARPC4 promotes bladder cancer cell invasion and is
            associated with lymph node metastasis.
REFERENCE   5  (bases 1 to 1433)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1433)
  AUTHORS   Robinson,R.C., Turbedsky,K., Kaiser,D.A., Marchand,J.B.,
            Higgs,H.N., Choe,S. and Pollard,T.D.
  TITLE     Crystal structure of Arp2/3 complex
  JOURNAL   Science 294 (5547), 1679-1684 (2001)
   PUBMED   11721045
REFERENCE   7  (bases 1 to 1433)
  AUTHORS   Gournier,H., Goley,E.D., Niederstrasser,H., Trinh,T. and Welch,M.D.
  TITLE     Reconstitution of human Arp2/3 complex reveals critical roles of
            individual subunits in complex structure and activity
  JOURNAL   Mol Cell 8 (5), 1041-1052 (2001)
   PUBMED   11741539
REFERENCE   8  (bases 1 to 1433)
  AUTHORS   Zhao,X., Yang,Z., Qian,M. and Zhu,X.
  TITLE     Interactions among subunits of human Arp2/3 complex: p20-Arc as the
            hub
  JOURNAL   Biochem Biophys Res Commun 280 (2), 513-517 (2001)
   PUBMED   11162547
REFERENCE   9  (bases 1 to 1433)
  AUTHORS   Machesky,L.M., Reeves,E., Wientjes,F., Mattheyse,F.J., Grogan,A.,
            Totty,N.F., Burlingame,A.L., Hsuan,J.J. and Segal,A.W.
  TITLE     Mammalian actin-related protein 2/3 complex localizes to regions of
            lamellipodial protrusion and is composed of evolutionarily
            conserved proteins
  JOURNAL   Biochem J 328 (Pt 1) (Pt 1), 105-112 (1997)
   PUBMED   9359840
REFERENCE   10 (bases 1 to 1433)
  AUTHORS   Welch,M.D., DePace,A.H., Verma,S., Iwamatsu,A. and Mitchison,T.J.
  TITLE     The human Arp2/3 complex is composed of evolutionarily conserved
            subunits and is localized to cellular regions of dynamic actin
            filament assembly
  JOURNAL   J Cell Biol 138 (2), 375-384 (1997)
   PUBMED   9230079
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA909066.1, AF006087.1,
            AC022382.4 and AI612891.1.
            
            On Aug 11, 2020 this sequence version replaced NM_005718.4.
            
            Summary: This gene encodes one of seven subunits of the human
            Arp2/3 protein complex. This complex controls actin polymerization
            in cells and has been conserved throughout eukaryotic evolution.
            This gene encodes the p20 subunit, which is necessary for actin
            nucleation and high-affinity binding to F-actin. Alternative
            splicing results in multiple transcript variants. Naturally
            occurring read-through transcription exists between this gene and
            the downstream tubulin tyrosine ligase-like family, member 3
            (TTLL3), which results in the production of a fusion protein.
            [provided by RefSeq, Nov 2010].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC065423.2, CD556183.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000397261.8/ ENSP00000380431.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                DA909066.1         513-537
            26-827              AF006087.1         1-802
            828-1021            AC022382.4         28841-29034         c
            1022-1433           AI612891.1         22-433              c
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.3"
     gene            1..1433
                     /gene="ARPC4"
                     /gene_synonym="ARC20; DEVLO; P20-ARC"
                     /note="actin related protein 2/3 complex subunit 4"
                     /db_xref="GeneID:10093"
                     /db_xref="HGNC:HGNC:707"
                     /db_xref="MIM:604226"
     exon            1..43
                     /gene="ARPC4"
                     /gene_synonym="ARC20; DEVLO; P20-ARC"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..547
                     /gene="ARPC4"
                     /gene_synonym="ARC20; DEVLO; P20-ARC"
                     /note="isoform a is encoded by transcript variant 1;
                     arp2/3 protein complex subunit p20; arp2/3 complex 20 kDa
                     subunit; actin related protein 2/3 complex, subunit 4,
                     20kDa"
                     /codon_start=1
                     /product="actin-related protein 2/3 complex subunit 4
                     isoform a"
                     /protein_id="NP_005709.1"
                     /db_xref="CCDS:CCDS43047.1"
                     /db_xref="GeneID:10093"
                     /db_xref="HGNC:HGNC:707"
                     /db_xref="MIM:604226"
                     /translation="MTATLRPYLSAVRATLQAALCLENFSSQVVERHNKPEVEVRSSK
                     ELLLQPVTISRNEKEKVLIEGSINSVRVSIAVKQADEIEKILCHKFMRFMMMRAENFF
                     ILRRKPVEGYDISFLITNFHTEQMYKHKLVDFVIHFMEEIDKEISEMKLSVNARARIV
                     AEEFLKNF"
     exon            44..162
                     /gene="ARPC4"
                     /gene_synonym="ARC20; DEVLO; P20-ARC"
                     /inference="alignment:Splign:2.1.0"
     exon            163..274
                     /gene="ARPC4"
                     /gene_synonym="ARC20; DEVLO; P20-ARC"
                     /inference="alignment:Splign:2.1.0"
     exon            275..370
                     /gene="ARPC4"
                     /gene_synonym="ARC20; DEVLO; P20-ARC"
                     /inference="alignment:Splign:2.1.0"
     exon            371..541
                     /gene="ARPC4"
                     /gene_synonym="ARC20; DEVLO; P20-ARC"
                     /inference="alignment:Splign:2.1.0"
     exon            542..1433
                     /gene="ARPC4"
                     /gene_synonym="ARC20; DEVLO; P20-ARC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1398..1403
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARPC4"
                     /gene_synonym="ARC20; DEVLO; P20-ARC"
                     /note="hexamer: ATTAAA"
     polyA_site      1433
                     /gene="ARPC4"
                     /gene_synonym="ARC20; DEVLO; P20-ARC"
                     /note="major polyA site"
ORIGIN      
        1 acttccgtac ttccgctttc cggcccagcc agcgcccgcg atgactgcca ctctccgccc
       61 ctacctgagt gccgtgcggg ccacattgca ggctgccctc tgcctggaga acttctcctc
      121 ccaggttgtg gaacgacaca acaagccgga agtggaagtc aggagtagca aagagctcct
      181 gttacaacct gtgaccatca gcaggaatga gaaggaaaag gttctgattg agggctccat
      241 caactctgtc cgggtcagca ttgctgtgaa acaggctgat gagatcgaga agattttgtg
      301 ccacaagttc atgcgcttca tgatgatgcg agcagagaac ttctttatcc ttcgaaggaa
      361 gcctgtggag gggtatgata tcagctttct gatcaccaac ttccacacag agcagatgta
      421 caaacacaag ttggtggact ttgtgatcca cttcatggag gagattgaca aggagatcag
      481 tgagatgaag ctgtcagtca atgcccgtgc ccgcattgtg gctgaagagt tccttaagaa
      541 tttttaaacc atctggctgg atctcgtggc cttccccctc agactaccca tgtctccacg
      601 aaggcgtcct ggagtcactc cccgagcagc gcggcggcgg cagggagttg ggttggggtg
      661 ggcatttgat gcgggaggtg ggtggtgtgc ttgctagctg ggcaagaaag cagcagtgga
      721 cctgccccaa ggccacacgt gcctggtcag gctggcttct gatgttcagt cccctgggcc
      781 gggacagatt ttttttaacg tcttgaaact taaactctgt gcttgtagga tactgtaacc
      841 tttttgtctt tttttttttt ttttttttta aacctccacc tccagtggct gtgactggtc
      901 ccagtgatta tacgttattg gttgctgtgg gtctgggcgg gcctggagcc agaaggcgac
      961 ttattttttc tctcctatgc caccccaggt ggggagggag ggaggtttca gactccagtt
     1021 tctcccttgc ccttttattc ccaagcctcc ttggccccag tataagtgtt gggaactctc
     1081 tgccagcttc ctgagttctg cctaaagatg gccccccagc actggttggg gacaagggcc
     1141 aaattggtga ccaccagtca tgcctaggac ctggggccca aaagccgggc aacctctggc
     1201 tacaggggcc attgggtgcc ctcccagttg agaggggccg ctgtttcgcc ttgtccgtca
     1261 gtgccagtgc ctgtgccaga ggtggcgagg tgagtcctcc agagcttcct ggccgggccc
     1321 tcctcctgcc tgttctggct cctctgctcc cacgctagct gtcccttttc tgattctggt
     1381 ggatcctccc tcccctaatt aaagtctctt tttgcccctt tggggctgca tga
//
