LOCUS       NM_001278556             946 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens actin related protein 2/3 complex subunit 3 (ARPC3),
            transcript variant 1, mRNA.
ACCESSION   NM_001278556 NM_005719
VERSION     NM_001278556.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Zhou,Y., Liu,Y., Gupta,S., Paramo,M.I., Hou,Y., Mao,C., Luo,Y.,
            Judd,J., Wierbowski,S., Bertolotti,M., Nerkar,M., Jehi,L.,
            Drayman,N., Nicolaescu,V., Gula,H., Tay,S., Randall,G., Wang,P.,
            Lis,J.T., Feschotte,C., Erzurum,S.C., Cheng,F. and Yu,H.
  TITLE     A comprehensive SARS-CoV-2-human protein-protein interactome
            reveals COVID-19 pathobiology and potential host therapeutic
            targets
  JOURNAL   Nat Biotechnol 41 (1), 128-139 (2023)
   PUBMED   36217030
REFERENCE   2  (bases 1 to 946)
  AUTHORS   Kim,D.K., Weller,B., Lin,C.W., Sheykhkarimli,D., Knapp,J.J.,
            Dugied,G., Zanzoni,A., Pons,C., Tofaute,M.J., Maseko,S.B.,
            Spirohn,K., Laval,F., Lambourne,L., Kishore,N., Rayhan,A.,
            Sauer,M., Young,V., Halder,H., la Rosa,N.M., Pogoutse,O.,
            Strobel,A., Schwehn,P., Li,R., Rothballer,S.T., Altmann,M.,
            Cassonnet,P., Cote,A.G., Vergara,L.E., Hazelwood,I., Liu,B.B.,
            Nguyen,M., Pandiarajan,R., Dohai,B., Coloma,P.A.R., Poirson,J.,
            Giuliana,P., Willems,L., Taipale,M., Jacob,Y., Hao,T., Hill,D.E.,
            Brun,C., Twizere,J.C., Krappmann,D., Heinig,M., Falter,C., Aloy,P.,
            Demeret,C., Vidal,M., Calderwood,M.A., Roth,F.P. and
            Falter-Braun,P.
  TITLE     A proteome-scale map of the SARS-CoV-2-human contactome
  JOURNAL   Nat Biotechnol 41 (1), 140-149 (2023)
   PUBMED   36217029
REFERENCE   3  (bases 1 to 946)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 946)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 946)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 946)
  AUTHORS   Zhao,X., Yang,Z., Qian,M. and Zhu,X.
  TITLE     Interactions among subunits of human Arp2/3 complex: p20-Arc as the
            hub
  JOURNAL   Biochem Biophys Res Commun 280 (2), 513-517 (2001)
   PUBMED   11162547
REFERENCE   7  (bases 1 to 946)
  AUTHORS   Marchand,J.B., Kaiser,D.A., Pollard,T.D. and Higgs,H.N.
  TITLE     Interaction of WASP/Scar proteins with actin and vertebrate Arp2/3
            complex
  JOURNAL   Nat Cell Biol 3 (1), 76-82 (2001)
   PUBMED   11146629
REFERENCE   8  (bases 1 to 946)
  AUTHORS   Machesky,L.M., Reeves,E., Wientjes,F., Mattheyse,F.J., Grogan,A.,
            Totty,N.F., Burlingame,A.L., Hsuan,J.J. and Segal,A.W.
  TITLE     Mammalian actin-related protein 2/3 complex localizes to regions of
            lamellipodial protrusion and is composed of evolutionarily
            conserved proteins
  JOURNAL   Biochem J 328 (Pt 1) (Pt 1), 105-112 (1997)
   PUBMED   9359840
REFERENCE   9  (bases 1 to 946)
  AUTHORS   Welch,M.D., DePace,A.H., Verma,S., Iwamatsu,A. and Mitchison,T.J.
  TITLE     The human Arp2/3 complex is composed of evolutionarily conserved
            subunits and is localized to cellular regions of dynamic actin
            filament assembly
  JOURNAL   J Cell Biol 138 (2), 375-384 (1997)
   PUBMED   9230079
REFERENCE   10 (bases 1 to 946)
  AUTHORS   Welch,M.D., Iwamatsu,A. and Mitchison,T.J.
  TITLE     Actin polymerization is induced by Arp2/3 protein complex at the
            surface of Listeria monocytogenes
  JOURNAL   Nature 385 (6613), 265-269 (1997)
   PUBMED   9000076
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BF434378.1, BC067747.1,
            AI144468.1 and AC144548.8.
            
            On Nov 22, 2018 this sequence version replaced NM_001278556.1.
            
            Summary: This gene encodes one of seven subunits of the human
            Arp2/3 protein complex. The Arp2/3 protein complex has been
            conserved through evolution and is implicated in the control of
            actin polymerization in cells. Alternatively spliced transcript
            variants have been found for this gene. [provided by RefSeq, Dec
            2013].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163655.121631.1,
                                           SRR5189658.196420.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000228825.12/ ENSP00000228825.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                BF434378.1         86-105
            21-646              BC067747.1         1-626
            647-879             AI144468.1         10-242              c
            880-946             AC144548.8         20822-20888
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.11"
     gene            1..946
                     /gene="ARPC3"
                     /gene_synonym="ARC21; p21-Arc"
                     /note="actin related protein 2/3 complex subunit 3"
                     /db_xref="GeneID:10094"
                     /db_xref="HGNC:HGNC:706"
                     /db_xref="MIM:604225"
     exon            1..83
                     /gene="ARPC3"
                     /gene_synonym="ARC21; p21-Arc"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..614
                     /gene="ARPC3"
                     /gene_synonym="ARC21; p21-Arc"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ARP2/3 protein complex subunit p21; arp2/3 complex 21 kDa
                     subunit; actin related protein 2/3 complex, subunit 3,
                     21kDa"
                     /codon_start=1
                     /product="actin-related protein 2/3 complex subunit 3
                     isoform 1"
                     /protein_id="NP_001265485.1"
                     /db_xref="CCDS:CCDS9146.1"
                     /db_xref="GeneID:10094"
                     /db_xref="HGNC:HGNC:706"
                     /db_xref="MIM:604225"
                     /translation="MPAYHSSLMDPDTKLIGNMALLPIRSQFKGPAPRETKDTDIVDE
                     AIYYFKANVFFKNYEIKNEADRTLIYITLYISECLKKLQKCNSKSQGEKEMYTLGITN
                     FPIPGEPGFPLNAIYAKPANKQEDEVMRAYLQQLRQETGLRLCEKVFDPQNDKPSKWW
                     TCFVKRQFMNKSLSGPGQ"
     exon            84..183
                     /gene="ARPC3"
                     /gene_synonym="ARC21; p21-Arc"
                     /inference="alignment:Splign:2.1.0"
     exon            184..260
                     /gene="ARPC3"
                     /gene_synonym="ARC21; p21-Arc"
                     /inference="alignment:Splign:2.1.0"
     exon            261..329
                     /gene="ARPC3"
                     /gene_synonym="ARC21; p21-Arc"
                     /inference="alignment:Splign:2.1.0"
     exon            330..456
                     /gene="ARPC3"
                     /gene_synonym="ARC21; p21-Arc"
                     /inference="alignment:Splign:2.1.0"
     exon            457..551
                     /gene="ARPC3"
                     /gene_synonym="ARC21; p21-Arc"
                     /inference="alignment:Splign:2.1.0"
     exon            552..946
                     /gene="ARPC3"
                     /gene_synonym="ARC21; p21-Arc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      845..850
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARPC3"
                     /gene_synonym="ARC21; p21-Arc"
                     /note="hexamer: AATAAA"
     regulatory      849..854
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARPC3"
                     /gene_synonym="ARC21; p21-Arc"
                     /note="hexamer: AAGAAA"
     polyA_site      868
                     /gene="ARPC3"
                     /gene_synonym="ARC21; p21-Arc"
                     /note="major polyA site"
     polyA_site      879
                     /gene="ARPC3"
                     /gene_synonym="ARC21; p21-Arc"
     regulatory      922..927
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARPC3"
                     /gene_synonym="ARC21; p21-Arc"
                     /note="hexamer: GATAAA"
     polyA_site      946
                     /gene="ARPC3"
                     /gene_synonym="ARC21; p21-Arc"
ORIGIN      
        1 gcccaggctg ccagaccgga agcgctccgc tgtacctgga tcctgctcct ctgggttgaa
       61 acccgggcgc cgccaagatg ccggcttacc actcttctct catggatcct gataccaaac
      121 tcatcggaaa catggcactg ttgcctatca gaagtcaatt caaaggacct gcccccagag
      181 agacaaaaga tacagatatt gtggatgaag ccatctatta cttcaaggcc aatgtcttct
      241 tcaaaaacta tgaaattaag aatgaagctg ataggacctt gatatatata actctctaca
      301 tttctgaatg tctgaagaaa ctgcaaaagt gcaattccaa aagccaaggt gagaaagaaa
      361 tgtatacgct gggaatcact aattttccca ttcctggaga gcctggtttt ccacttaacg
      421 caatttatgc caaacctgca aacaaacagg aagatgaagt gatgagagcc tatttacaac
      481 agctaaggca agagactgga ctgagacttt gtgagaaagt tttcgaccct cagaatgata
      541 aacccagcaa gtggtggact tgctttgtga agagacagtt catgaacaag agtctttcag
      601 gacctggaca gtgaagggag cccgggcagc caccgtctcc agagccctgg gcagcatttt
      661 ccagcaagat gtacacaatc ttttgccttt atttcgtaaa gttttataca gaagagagaa
      721 gagcatgtct ttacttgaaa aactcttgat caagaatttg ggtgggagaa aagaaagtgg
      781 gttatcaagg gtgatttgaa attttctgca gcattaaagc tggcgcttaa taagaataag
      841 taataataaa gaaatttcta acattccatg tcagaaaaac tttattgcca gtctttgaca
      901 ttcacttaca atttgttttg agataaaatt cacattccat aatgcc
//
