LOCUS       NM_152862               1410 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens actin related protein 2/3 complex subunit 2 (ARPC2),
            transcript variant 1, mRNA.
ACCESSION   NM_152862
VERSION     NM_152862.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Poh,J., Ponsford,A.H., Boyd,J., Woodsmith,J., Stelzl,U., Wanker,E.,
            Harper,N., MacEwan,D. and Sanderson,C.M.
  TITLE     A functionally defined high-density NRF2 interactome reveals new
            conditional regulators of ARE transactivation
  JOURNAL   Redox Biol 37, 101686 (2020)
   PUBMED   32911434
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Skruber,K., Warp,P.V., Shklyarov,R., Thomas,J.D., Swanson,M.S.,
            Henty-Ridilla,J.L., Read,T.A. and Vitriol,E.A.
  TITLE     Arp2/3 and Mena/VASP Require Profilin 1 for Actin Network Assembly
            at the Leading Edge
  JOURNAL   Curr Biol 30 (14), 2651-2664 (2020)
   PUBMED   32470361
  REMARK    GeneRIF: Arp2/3 and Mena/VASP Require Profilin 1 for Actin Network
            Assembly at the Leading Edge.
REFERENCE   3  (bases 1 to 1410)
  AUTHORS   Al-Zeer,M.A., Dutkiewicz,M., von Hacht,A., Kreuzmann,D., Rohrs,V.
            and Kurreck,J.
  TITLE     Alternatively spliced variants of the 5'-UTR of the ARPC2 mRNA
            regulate translation by an internal ribosome entry site (IRES)
            harboring a guanine-quadruplex motif
  JOURNAL   RNA Biol 16 (11), 1622-1632 (2019)
   PUBMED   31387452
  REMARK    GeneRIF: Study provides evidence that the 5'-UTR1 of ARPC2 has
            internal ribosome entry site activity and regulates translation
            during stressful cellular conditions such as high cell density.
REFERENCE   4  (bases 1 to 1410)
  AUTHORS   Cheng,Z., Wei,W., Wu,Z., Wang,J., Ding,X., Sheng,Y., Han,Y. and
            Wu,Q.
  TITLE     ARPC2 promotes breast cancer proliferation and metastasis
  JOURNAL   Oncol Rep 41 (6), 3189-3200 (2019)
   PUBMED   31002363
  REMARK    GeneRIF: These results collectively indicated that ARPC2 promoted
            the tumorigenesis of breast carcinoma and the initiation of
            epithelialmesenchymal transition. Therefore, ARPC2 was revealed to
            be a potential therapeutic target in patients with breast cancer
            (BrCa) .
REFERENCE   5  (bases 1 to 1410)
  AUTHORS   Chen,P., Yue,X., Xiong,H., Lu,X. and Ji,Z.
  TITLE     RBM3 upregulates ARPC2 by binding the 3'UTR and contributes to
            breast cancer progression
  JOURNAL   Int J Oncol 54 (4), 1387-1397 (2019)
   PUBMED   30720048
  REMARK    GeneRIF: Results find that ARPC2 expression is upregulated in
            breast cancer tissues and cell lines. ARPC2 promotes the
            proliferation and metastasis of human breast cancer cells. ARPC2 is
            regulated by RBM2 in a posttranscriptional 3'UTRbinding manner.
            Also, ARPC2 plays an oncogenic role and mediates the promoting role
            of RBM3 in the proliferation and metastasis of human breast cancer
            cells.
REFERENCE   6  (bases 1 to 1410)
  AUTHORS   Robinson,R.C., Turbedsky,K., Kaiser,D.A., Marchand,J.B.,
            Higgs,H.N., Choe,S. and Pollard,T.D.
  TITLE     Crystal structure of Arp2/3 complex
  JOURNAL   Science 294 (5547), 1679-1684 (2001)
   PUBMED   11721045
REFERENCE   7  (bases 1 to 1410)
  AUTHORS   Zhao,X., Yang,Z., Qian,M. and Zhu,X.
  TITLE     Interactions among subunits of human Arp2/3 complex: p20-Arc as the
            hub
  JOURNAL   Biochem Biophys Res Commun 280 (2), 513-517 (2001)
   PUBMED   11162547
REFERENCE   8  (bases 1 to 1410)
  AUTHORS   Naar,A.M., Beaurang,P.A., Zhou,S., Abraham,S., Solomon,W. and
            Tjian,R.
  TITLE     Composite co-activator ARC mediates chromatin-directed
            transcriptional activation
  JOURNAL   Nature 398 (6730), 828-832 (1999)
   PUBMED   10235267
REFERENCE   9  (bases 1 to 1410)
  AUTHORS   Machesky,L.M., Reeves,E., Wientjes,F., Mattheyse,F.J., Grogan,A.,
            Totty,N.F., Burlingame,A.L., Hsuan,J.J. and Segal,A.W.
  TITLE     Mammalian actin-related protein 2/3 complex localizes to regions of
            lamellipodial protrusion and is composed of evolutionarily
            conserved proteins
  JOURNAL   Biochem J 328 (Pt 1) (Pt 1), 105-112 (1997)
   PUBMED   9359840
REFERENCE   10 (bases 1 to 1410)
  AUTHORS   Welch,M.D., DePace,A.H., Verma,S., Iwamatsu,A. and Mitchison,T.J.
  TITLE     The human Arp2/3 complex is composed of evolutionarily conserved
            subunits and is localized to cellular regions of dynamic actin
            filament assembly
  JOURNAL   J Cell Biol 138 (2), 375-384 (1997)
   PUBMED   9230079
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG282181.1, AK290268.1 and
            CN291458.1.
            
            On Nov 23, 2018 this sequence version replaced NM_152862.2.
            
            Summary: This gene encodes one of seven subunits of the human
            Arp2/3 protein complex. The Arp2/3 protein complex has been
            implicated in the control of actin polymerization in cells and has
            been conserved through evolution. The exact role of the protein
            encoded by this gene, the p34 subunit, has yet to be determined.
            Two alternatively spliced variants have been characterized to date.
            Additional alternatively spliced variants have been described but
            their full length nature has not been determined. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 both encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.750238.1,
                                           SRR5189652.54299.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000315717.10/ ENSP00000327137.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 BG282181.1         96-101
            7-1160              AK290268.1         1-1154
            1161-1410           CN291458.1         153-402
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q35"
     gene            1..1410
                     /gene="ARPC2"
                     /gene_synonym="ARC34; p34-Arc; PNAS-139; PRO2446"
                     /note="actin related protein 2/3 complex subunit 2"
                     /db_xref="GeneID:10109"
                     /db_xref="HGNC:HGNC:705"
                     /db_xref="MIM:604224"
     exon            1..66
                     /gene="ARPC2"
                     /gene_synonym="ARC34; p34-Arc; PNAS-139; PRO2446"
                     /inference="alignment:Splign:2.1.0"
     exon            67..148
                     /gene="ARPC2"
                     /gene_synonym="ARC34; p34-Arc; PNAS-139; PRO2446"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..977
                     /gene="ARPC2"
                     /gene_synonym="ARC34; p34-Arc; PNAS-139; PRO2446"
                     /note="ARP2/3 protein complex subunit 34; arp2/3 complex
                     34 kDa subunit; actin related protein 2/3 complex, subunit
                     2, 34kDa; testis tissue sperm-binding protein Li 53e"
                     /codon_start=1
                     /product="actin-related protein 2/3 complex subunit 2"
                     /protein_id="NP_690601.1"
                     /db_xref="CCDS:CCDS2410.1"
                     /db_xref="GeneID:10109"
                     /db_xref="HGNC:HGNC:705"
                     /db_xref="MIM:604224"
                     /translation="MILLEVNNRIIEETLALKFENAAAGNKPEAVEVTFADFDGVLYH
                     ISNPNGDKTKVMVSISLKFYKELQAHGADELLKRVYGSFLVNPESGYNVSLLYDLENL
                     PASKDSIVHQAGMLKRNCFASVFEKYFQFQEEGKEGENRAVIHYRDDETMYVESKKDR
                     VTVVFSTVFKDDDDVVIGKVFMQEFKEGRRASHTAPQVLFSHREPPLELKDTDAAVGD
                     NIGYITFVLFPRHTNASARDNTINLIHTFRDYLHYHIKCSKAYIHTRMRAKTSDFLKV
                     LNRARPDAEKKEMKTITGKTFSSR"
     exon            149..183
                     /gene="ARPC2"
                     /gene_synonym="ARC34; p34-Arc; PNAS-139; PRO2446"
                     /inference="alignment:Splign:2.1.0"
     exon            184..296
                     /gene="ARPC2"
                     /gene_synonym="ARC34; p34-Arc; PNAS-139; PRO2446"
                     /inference="alignment:Splign:2.1.0"
     exon            297..342
                     /gene="ARPC2"
                     /gene_synonym="ARC34; p34-Arc; PNAS-139; PRO2446"
                     /inference="alignment:Splign:2.1.0"
     exon            343..529
                     /gene="ARPC2"
                     /gene_synonym="ARC34; p34-Arc; PNAS-139; PRO2446"
                     /inference="alignment:Splign:2.1.0"
     exon            530..623
                     /gene="ARPC2"
                     /gene_synonym="ARC34; p34-Arc; PNAS-139; PRO2446"
                     /inference="alignment:Splign:2.1.0"
     exon            624..750
                     /gene="ARPC2"
                     /gene_synonym="ARC34; p34-Arc; PNAS-139; PRO2446"
                     /inference="alignment:Splign:2.1.0"
     exon            751..851
                     /gene="ARPC2"
                     /gene_synonym="ARC34; p34-Arc; PNAS-139; PRO2446"
                     /inference="alignment:Splign:2.1.0"
     exon            852..952
                     /gene="ARPC2"
                     /gene_synonym="ARC34; p34-Arc; PNAS-139; PRO2446"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1410
                     /gene="ARPC2"
                     /gene_synonym="ARC34; p34-Arc; PNAS-139; PRO2446"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1387..1392
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARPC2"
                     /gene_synonym="ARC34; p34-Arc; PNAS-139; PRO2446"
                     /note="hexamer: AATAAA"
     polyA_site      1410
                     /gene="ARPC2"
                     /gene_synonym="ARC34; p34-Arc; PNAS-139; PRO2446"
                     /note="major polyA site"
ORIGIN      
        1 gtcggtgaag cggcagtggc ggcggcggcg gcggctcggc aggcgggttc aggcttcggg
       61 ggccagccgc cgccatgatc ctgctggagg tgaacaaccg catcatcgag gagacgctcg
      121 cgctcaagtt cgagaacgcg gccgccggaa acaaaccgga agcagtagaa gtaacatttg
      181 cagatttcga tggggtcctc tatcatattt caaatcctaa tggagacaaa acaaaagtga
      241 tggtcagtat ttctttgaaa ttctacaagg aacttcaggc acatggtgct gatgagttat
      301 taaagagggt gtacgggagt ttcttggtaa atccagaatc aggatacaat gtctctttgc
      361 tatatgacct tgaaaatctt ccggcatcca aggattccat tgtgcatcaa gctggcatgt
      421 tgaagcgaaa ttgttttgcc tctgtctttg aaaaatactt ccaattccaa gaagagggca
      481 aggaaggaga gaacagggca gttatccatt atagggatga tgagaccatg tatgttgagt
      541 ctaaaaagga cagagtcaca gtagtcttca gcacagtgtt taaggatgac gacgatgtgg
      601 tcattggaaa ggtgttcatg caggagttca aagaaggacg cagagccagc cacacagccc
      661 cacaggtcct ctttagccac agggaacctc ctctggagct gaaagacaca gacgccgctg
      721 tgggtgacaa cattggctac attacctttg tgctgttccc tcgtcacacc aatgccagtg
      781 ctcgagacaa caccatcaac ctgatccaca cgttccggga ctacctgcac taccacatca
      841 agtgctctaa ggcctatatt cacacacgta tgcgggcgaa aacgtctgac ttcctcaagg
      901 tgctgaaccg cgcacgccca gatgccgaga aaaaagaaat gaaaacaatc acggggaaga
      961 cgttttcatc ccgctaatct tgggaataag aggaggaagc ggctggcaac tgaaggctgg
     1021 aacacttgct actggataat cgtagctttt aatgttgcgc ctcttcaggt tcttaaggga
     1081 ttctccgttt tggttccatt ttgtacacgt ttggaaaata atctgcagaa acgagctgtg
     1141 cttgcaaaga cttcatagtt cccaagaatt aaaaaaaaaa aaaaaagaat tccacttgat
     1201 caacttaatt ccttttcttt atcttccctc cctcacttcc cttttctccc accctctttt
     1261 ccaagctgtt tcgctttgca atatattact ggtaatgagt tgcaggataa tgcagtcata
     1321 acttgttttc tcctaagtat ttgagttcaa aactcctgta tctaaagaaa tacggttggg
     1381 gtcattaata aagaaaatct ttctatctta
//
