LOCUS       NM_138795               1789 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens ARF like GTPase 8A (ARL8A), transcript variant 1,
            mRNA.
ACCESSION   NM_138795
VERSION     NM_138795.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1789)
  AUTHORS   Marwaha,R., Arya,S.B., Jagga,D., Kaur,H., Tuli,A. and Sharma,M.
  TITLE     The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to
            lysosomes
  JOURNAL   J Cell Biol 216 (4), 1051-1070 (2017)
   PUBMED   28325809
REFERENCE   4  (bases 1 to 1789)
  AUTHORS   Pu,J., Schindler,C., Jia,R., Jarnik,M., Backlund,P. and
            Bonifacino,J.S.
  TITLE     BORC, a multisubunit complex that regulates lysosome positioning
  JOURNAL   Dev Cell 33 (2), 176-188 (2015)
   PUBMED   25898167
REFERENCE   5  (bases 1 to 1789)
  AUTHORS   Yu,X., Bian,X., Throop,A., Song,L., Moral,L.D., Park,J., Seiler,C.,
            Fiacco,M., Steel,J., Hunter,P., Saul,J., Wang,J., Qiu,J.,
            Pipas,J.M. and LaBaer,J.
  TITLE     Exploration of panviral proteome: high-throughput cloning and
            functional implications in virus-host interactions
  JOURNAL   Theranostics 4 (8), 808-822 (2014)
   PUBMED   24955142
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1789)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   7  (bases 1 to 1789)
  AUTHORS   Schroder,B., Wrocklage,C., Pan,C., Jager,R., Kosters,B.,
            Schafer,H., Elsasser,H.P., Mann,M. and Hasilik,A.
  TITLE     Integral and associated lysosomal membrane proteins
  JOURNAL   Traffic 8 (12), 1676-1686 (2007)
   PUBMED   17897319
REFERENCE   8  (bases 1 to 1789)
  AUTHORS   Hofmann,I. and Munro,S.
  TITLE     An N-terminally acetylated Arf-like GTPase is localised to
            lysosomes and affects their motility
  JOURNAL   J Cell Sci 119 (Pt 8), 1494-1503 (2006)
   PUBMED   16537643
  REMARK    GeneRIF: Overexpression of Arl8a or Arl8b results in a
            microtubule-dependent redistribution of lysosomes towards the cell
            periphery.  Live cell imaging suggested a role for Arl8a and Arl8b
            as positive regulators of lysosomal transport.[Ar18a] [Ar18b]
REFERENCE   9  (bases 1 to 1789)
  AUTHORS   Okai,T., Araki,Y., Tada,M., Tateno,T., Kontani,K. and Katada,T.
  TITLE     Novel small GTPase subfamily capable of associating with tubulin is
            required for chromosome segregation
  JOURNAL   J Cell Sci 117 (Pt 20), 4705-4715 (2004)
   PUBMED   15331635
  REMARK    GeneRIF: Results suggest that the novel GTPases Gie1 and Gie2
            associate with microtubules, and might be involved in chromosome
            segregation.
REFERENCE   10 (bases 1 to 1789)
  AUTHORS   Secombe,J. and Parkhurst,S.M.
  TITLE     Drosophila Topors is a RING finger-containing protein that
            functions as a ubiquitin-protein isopeptide ligase for the hairy
            basic helix-loop-helix repressor protein
  JOURNAL   J Biol Chem 279 (17), 17126-17133 (2004)
   PUBMED   14871887
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP325806.1, BC015408.1, BC069053.1 and AW090198.1.
            
            On Nov 23, 2018 this sequence version replaced NM_138795.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK127138.1, SRR1163657.222254.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467146 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000272217.7/ ENSP00000272217.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-63                BP325806.1         7-69
            64-932              BC015408.1         1-869
            933-1786            BC069053.1         31-884
            1787-1789           AW090198.1         21-23               c
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.1"
     gene            1..1789
                     /gene="ARL8A"
                     /gene_synonym="ARL10B; GIE2"
                     /note="ARF like GTPase 8A"
                     /db_xref="GeneID:127829"
                     /db_xref="HGNC:HGNC:25192"
                     /db_xref="MIM:616597"
     exon            1..288
                     /gene="ARL8A"
                     /gene_synonym="ARL10B; GIE2"
                     /inference="alignment:Splign:2.1.0"
     CDS             166..726
                     /gene="ARL8A"
                     /gene_synonym="ARL10B; GIE2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ADP-ribosylation factor-like 10B; ADP-ribosylation
                     factor-like protein 8A; novel small G protein
                     indispensable for equal chromosome segregation 2; ADP
                     ribosylation factor like GTPase 8A; ADP-ribosylation
                     factor-like 8A; GTPase indispensable for equal segregation
                     of chromosomes 2"
                     /codon_start=1
                     /product="ADP-ribosylation factor-like protein 8A isoform
                     1"
                     /protein_id="NP_620150.1"
                     /db_xref="CCDS:CCDS1421.1"
                     /db_xref="GeneID:127829"
                     /db_xref="HGNC:HGNC:25192"
                     /db_xref="MIM:616597"
                     /translation="MIALFNKLLDWFKALFWKEEMELTLVGLQYSGKTTFVNVIASGQ
                     FNEDMIPTVGFNMRKITKGNVTIKLWDIGGQPRFRSMWERYCRGVSAIVYMVDAADQE
                     KIEASKNELHNLLDKPQLQGIPVLVLGNKRDLPGALDEKELIEKMNLSAIQDREICCY
                     SISCKEKDNIDITLQWLIQHSKSRRS"
     exon            289..369
                     /gene="ARL8A"
                     /gene_synonym="ARL10B; GIE2"
                     /inference="alignment:Splign:2.1.0"
     exon            370..443
                     /gene="ARL8A"
                     /gene_synonym="ARL10B; GIE2"
                     /inference="alignment:Splign:2.1.0"
     exon            444..537
                     /gene="ARL8A"
                     /gene_synonym="ARL10B; GIE2"
                     /inference="alignment:Splign:2.1.0"
     exon            538..605
                     /gene="ARL8A"
                     /gene_synonym="ARL10B; GIE2"
                     /inference="alignment:Splign:2.1.0"
     exon            606..676
                     /gene="ARL8A"
                     /gene_synonym="ARL10B; GIE2"
                     /inference="alignment:Splign:2.1.0"
     exon            677..1789
                     /gene="ARL8A"
                     /gene_synonym="ARL10B; GIE2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      903..908
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARL8A"
                     /gene_synonym="ARL10B; GIE2"
                     /note="hexamer: AATAAA"
     polyA_site      932
                     /gene="ARL8A"
                     /gene_synonym="ARL10B; GIE2"
                     /note="major polyA site"
     regulatory      1765..1770
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARL8A"
                     /gene_synonym="ARL10B; GIE2"
                     /note="hexamer: AATAAA"
     polyA_site      1789
                     /gene="ARL8A"
                     /gene_synonym="ARL10B; GIE2"
                     /note="major polyA site"
ORIGIN      
        1 gagccgattc cggggcagcg agtcgtcggc agccgcgctc gagcctccgc ccgcaccgag
       61 ccgcgggacc cgggccgtac cggggagggg ccgctccggg ccgcagcgcg agggcagcga
      121 ggggcggcgg ggacctggca ccgggcgggg ccggcggcag cgaccatgat cgctttgttc
      181 aacaagctgc tggactggtt caaggcccta ttctggaagg aggagatgga gctcacgctg
      241 gtcgggcttc agtactcggg caagaccacc ttcgtcaacg tgatcgcgtc aggacagttc
      301 aacgaggaca tgatccccac cgtgggtttc aacatgcgca aaatcaccaa agggaatgtg
      361 actatcaagc tctgggacat tgggggacag ccgcgtttcc gcagcatgtg ggagcgctac
      421 tgccgaggag tgagcgccat cgtgtacatg gtggatgctg ctgaccagga gaagattgag
      481 gcctctaaga acgagctcca caacctactg gacaaacctc agctgcaggg catcccggtc
      541 ttagtcctgg gtaacaagcg agaccttccg ggagcattgg atgagaagga gctgattgag
      601 aaaatgaatc tgtctgccat ccaggaccga gagatctgct gctactccat ctcttgcaaa
      661 gaaaaggaca acattgacat caccctacag tggcttattc aacactcgaa gtcacggaga
      721 agctgagact ccagcccttc tccctcagac cagggaccgt catcatctaa acctgaagcc
      781 gagctccccg cccacccctg tcgtccccct aagcccaccc ctcctcaccc agtgtgagga
      841 gggccctctg gggaccccag agtcctgttc tgctgaggtt tgaactcctg tttttattgt
      901 aaaataaatt gccccccatt ctggtcccct aacttctcac ccttccccgc tgcctttgtc
      961 ccatcaccca gccctgcctc cctcccagca gccctgggcc acagcccccg cccctggctt
     1021 ttccccggcc cgctcttgta cctccctttt caacactctc tgttattgtc ctgtgtgtac
     1081 agtatatata tgtatatata ttttaatttt ttaatttaag caaagactaa aatcaaccat
     1141 ttgatgctgc aggggccttt caggatctgg gagggggcag tctggagaga aggagggaga
     1201 cgcaggtgga cttggggcaa gttcagatca gaagaggtgc aggctggcac ctgcggcagg
     1261 taccagcctg ggcactggtg gccgcctccc tgtcccgtgt gtttccaccg cccaatctgg
     1321 cttgtcctgg cagtgcttga atgccacagg ctggcagggg cctctggggg cccctcccct
     1381 cgacccccag cctgggtaga gccaccaggt acgacgacca ggtaccagaa accaccaggc
     1441 acacggggca gaaagccagc gtccatgccc cagcagcccc ctcctgcctg ttcctggctc
     1501 ccagctcccg cccctcccca gggcccccac ctccacggcc cacttcattt tctgttctca
     1561 ttttgcagag ttgcacaagg agagaactca gcatgggggg ttggttcttt gggttctgtt
     1621 tgtttatttg tttaatttaa tgatttgtaa agtgatgttc ctcttccttt tttacacttt
     1681 tcagctcata tttaacctct gtttggaaaa tgattcttgt aactgtacat ttttttgctt
     1741 cctaataaca atgacaacaa aaaaaataaa tgaccagttt tgtgttggg
//
