LOCUS       NP_659492                404 aa            linear   PRI 12-JUN-2025
DEFINITION  protein ARK2N isoform a [Homo sapiens].
ACCESSION   NP_659492
VERSION     NP_659492.1
DBSOURCE    REFSEQ: accession NM_145055.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 404)
  AUTHORS   Blazev,R., Carl,C.S., Ng,Y.K., Molendijk,J., Voldstedlund,C.T.,
            Zhao,Y., Xiao,D., Kueh,A.J., Miotto,P.M., Haynes,V.R., Hardee,J.P.,
            Chung,J.D., McNamara,J.W., Qian,H., Gregorevic,P., Oakhill,J.S.,
            Herold,M.J., Jensen,T.E., Lisowski,L., Lynch,G.S., Dodd,G.T.,
            Watt,M.J., Yang,P., Kiens,B., Richter,E.A. and Parker,B.L.
  TITLE     Phosphoproteomics of three exercise modalities identifies canonical
            signaling and C18ORF25 as an AMPK substrate regulating skeletal
            muscle function
  JOURNAL   Cell Metab 34 (10), 1561-1577 (2022)
   PUBMED   35882232
  REMARK    GeneRIF: Phosphoproteomics of three exercise modalities identifies
            canonical signaling and C18ORF25 as an AMPK substrate regulating
            skeletal muscle function.
REFERENCE   2  (residues 1 to 404)
  AUTHORS   Bernaudat,F., Gustems,M., Gunther,J., Oliva,M.F., Buschle,A.,
            Gobel,C., Pagniez,P., Lupo,J., Signor,L., Muller,C.W., Morand,P.,
            Sattler,M., Hammerschmidt,W. and Petosa,C.
  TITLE     Structural basis of DNA methylation-dependent site selectivity of
            the Epstein-Barr virus lytic switch protein ZEBRA/Zta/BZLF1
  JOURNAL   Nucleic Acids Res 50 (1), 490-511 (2022)
   PUBMED   34893887
REFERENCE   3  (residues 1 to 404)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   4  (residues 1 to 404)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (residues 1 to 404)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (residues 1 to 404)
  AUTHORS   Siddiqi,U.Z., Vaidya,A.S., Li,X., Marcon,E., Tsao,S.W.,
            Greenblatt,J. and Frappier,L.
  TITLE     Identification of ARKL1 as a Negative Regulator of Epstein-Barr
            Virus Reactivation
  JOURNAL   J Virol 93 (20), e00989-19 (2019)
   PUBMED   31341047
  REMARK    Publication Status: Online-Only
REFERENCE   7  (residues 1 to 404)
  AUTHORS   Cao,J.Y., Shire,K., Landry,C., Gish,G.D., Pawson,T. and Frappier,L.
  TITLE     Identification of a novel protein interaction motif in the
            regulatory subunit of casein kinase 2
  JOURNAL   Mol Cell Biol 34 (2), 246-258 (2014)
   PUBMED   24216761
  REMARK    GeneRIF: ARKL1 binds CK2beta through the KSSR motif and this
            involves a polyserine sequence resembling the CK2beta binding
            sequence in EBNA1.
REFERENCE   8  (residues 1 to 404)
  AUTHORS   Trynka,G., Zhernakova,A., Romanos,J., Franke,L., Hunt,K.A.,
            Turner,G., Bruinenberg,M., Heap,G.A., Platteel,M., Ryan,A.W., de
            Kovel,C., Holmes,G.K., Howdle,P.D., Walters,J.R., Sanders,D.S.,
            Mulder,C.J., Mearin,M.L., Verbeek,W.H., Trimble,V., Stevens,F.M.,
            Kelleher,D., Barisani,D., Bardella,M.T., McManus,R., van Heel,D.A.
            and Wijmenga,C.
  TITLE     Coeliac disease-associated risk variants in TNFAIP3 and REL
            implicate altered NF-kappaB signalling
  JOURNAL   Gut 58 (8), 1078-1083 (2009)
   PUBMED   19240061
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (residues 1 to 404)
  AUTHORS   Pickard,B.S., Malloy,M.P., Clark,L., Lehellard,S., Ewald,H.L.,
            Mors,O., Porteous,D.J., Blackwood,D.H. and Muir,W.J.
  TITLE     Candidate psychiatric illness genes identified in patients with
            pericentric inversions of chromosome 18
  JOURNAL   Psychiatr Genet 15 (1), 37-44 (2005)
   PUBMED   15722956
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC118647.4, AC021763.10 and KF573673.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BK005125.1, SRR11853567.14461.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000615052.5/ ENSP00000481626.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.1"
     Protein         1..404
                     /product="protein ARK2N isoform a"
                     /note="ARKadia-like 1; uncharacterized protein C18orf25;
                     ARKadia-like protein 1; ARKadia (RNF111) N-terminal like
                     PKA signaling regulator protein 2N; protein ARK2N"
                     /calculated_mol_wt=43264
     Region          1..50
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q96B23.3)"
     Region          63..255
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q96B23.3)"
     Site            66
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:20068231,
                     ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163;
                     propagated from UniProtKB/Swiss-Prot (Q96B23.3)"
     Site            67
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by AMPK.
                     /evidence=ECO:0000269|PubMed:35882232,
                     ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q96B23.3)"
     Region          <88..225
                     /region_name="RNF111_N"
                     /note="E3 ubiquitin-protein ligase Arkadia N-terminus;
                     pfam15303"
                     /db_xref="CDD:464629"
     Site            143
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21406692; propagated from
                     UniProtKB/Swiss-Prot (Q96B23.3)"
     Site            145
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21406692; propagated from
                     UniProtKB/Swiss-Prot (Q96B23.3)"
     Site            147
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8BH50; propagated from
                     UniProtKB/Swiss-Prot (Q96B23.3)"
     Region          202..226
                     /region_name="Required for interaction with CSNK2B.
                     /evidence=ECO:0000269|PubMed:24216761"
                     /note="propagated from UniProtKB/Swiss-Prot (Q96B23.3)"
     Site            327
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648,
                     ECO:0007744|PubMed:21406692; propagated from
                     UniProtKB/Swiss-Prot (Q96B23.3)"
     Site            328
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648,
                     ECO:0007744|PubMed:21406692; propagated from
                     UniProtKB/Swiss-Prot (Q96B23.3)"
     Site            330
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648,
                     ECO:0007744|PubMed:21406692; propagated from
                     UniProtKB/Swiss-Prot (Q96B23.3)"
     Site            347
                     /site_type="methylation"
                     /note="Omega-N-methylarginine.
                     /evidence=ECO:0007744|PubMed:24129315; propagated from
                     UniProtKB/Swiss-Prot (Q96B23.3)"
     CDS             1..404
                     /gene="ARK2N"
                     /gene_synonym="ARKL1; C18orf25; RNF111L1"
                     /coded_by="NM_145055.5:368..1582"
                     /note="isoform a is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS42430.1"
                     /db_xref="GeneID:147339"
                     /db_xref="HGNC:HGNC:28172"
ORIGIN      
        1 mkmeeavgkv eelieseapp kaseqetake edgsvelesq vqkdgvadst vissmpcllm
       61 elrrdssesq lastesdkpt tgrvyesdss nhcmlspsss ghladsdtls saeenepsqa
      121 etavegdpsg vsgatvgrks rrsrsesets tmaakknrqs sdkqngrvak vkghrsqkhk
      181 erirllrqkr eaaarkkynl lqdsstsdsd ltcdsstsss dddeevsgss ktitaeipdg
      241 ppvvahydms dtnsdpevvn vdnllaaavv qehsnsvggq dtgatwrtsg lleelnaeag
      301 hldpgflasd ktsagnapln eeiniassds eveivgvqeh arcvhprggv iqsvsswkhg
      361 sgtqyvstrq tqswtavtpq qtwaspaevv dltldedsrr kyll
//
