LOCUS       NM_006321               4912 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens ariadne RBR E3 ubiquitin protein ligase 2 (ARIH2),
            transcript variant 2, mRNA.
ACCESSION   NM_006321
VERSION     NM_006321.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Xu,P., Liu,Y., Liu,C., Guey,B., Li,L., Melenec,P., Ricci,J. and
            Ablasser,A.
  TITLE     The CRL5-SPSB3 ubiquitin ligase targets nuclear cGAS for
            degradation
  JOURNAL   Nature 627 (8005), 873-879 (2024)
   PUBMED   38418882
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Geng,S., Peng,W., Wang,X., Hu,X., Liang,H., Hou,J., Wang,F.,
            Zhao,G., Lu,M. and Cui,H.
  TITLE     ARIH2 regulates the proliferation, DNA damage and chemosensitivity
            of gastric cancer cells by reducing the stability of p21 via
            ubiquitination
  JOURNAL   Cell Death Dis 13 (6), 564 (2022)
   PUBMED   35732617
  REMARK    GeneRIF: ARIH2 regulates the proliferation, DNA damage and
            chemosensitivity of gastric cancer cells by reducing the stability
            of p21 via ubiquitination.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4912)
  AUTHORS   Kostrhon,S., Prabu,J.R., Baek,K., Horn-Ghetko,D., von Gronau,S.,
            Klugel,M., Basquin,J., Alpi,A.F. and Schulman,B.A.
  TITLE     CUL5-ARIH2 E3-E3 ubiquitin ligase structure reveals cullin-specific
            NEDD8 activation
  JOURNAL   Nat Chem Biol 17 (10), 1075-1083 (2021)
   PUBMED   34518685
REFERENCE   4  (bases 1 to 4912)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 4912)
  AUTHORS   Lumpkin,R.J., Ahmad,A.S., Blake,R., Condon,C.J. and Komives,E.A.
  TITLE     The Mechanism of NEDD8 Activation of CUL5 Ubiquitin E3 Ligases
  JOURNAL   Mol Cell Proteomics 20, 100019 (2021)
   PUBMED   33268465
REFERENCE   6  (bases 1 to 4912)
  AUTHORS   Marteijn,J.A., van der Meer,L.T., van Emst,L., van Reijmersdal,S.,
            Wissink,W., de Witte,T., Jansen,J.H. and Van der Reijden,B.A.
  TITLE     Gfi1 ubiquitination and proteasomal degradation is inhibited by the
            ubiquitin ligase Triad1
  JOURNAL   Blood 110 (9), 3128-3135 (2007)
   PUBMED   17646546
  REMARK    GeneRIF: The fine-tuning of Gfi1 protein levels regulated by Triad1
            defines an unexpected role for this protein in hematopoiesis.
REFERENCE   7  (bases 1 to 4912)
  AUTHORS   Marteijn,J.A., van Emst,L., Erpelinck-Verschueren,C.A.,
            Nikoloski,G., Menke,A., de Witte,T., Lowenberg,B., Jansen,J.H. and
            van der Reijden,B.A.
  TITLE     The E3 ubiquitin-protein ligase Triad1 inhibits clonogenic growth
            of primary myeloid progenitor cells
  JOURNAL   Blood 106 (13), 4114-4123 (2005)
   PUBMED   16118314
  REMARK    GeneRIF: proteasomal degradation of proteins that are ubiquitinated
            by Triad1 affects the clonogenic growth of primary myeloid
            progenitor cells
REFERENCE   8  (bases 1 to 4912)
  AUTHORS   Hu,R.M., Han,Z.G., Song,H.D., Peng,Y.D., Huang,Q.H., Ren,S.X.,
            Gu,Y.J., Huang,C.H., Li,Y.B., Jiang,C.L., Fu,G., Zhang,Q.H.,
            Gu,B.W., Dai,M., Mao,Y.F., Gao,G.F., Rong,R., Ye,M., Zhou,J.,
            Xu,S.H., Gu,J., Shi,J.X., Jin,W.R., Zhang,C.K., Wu,T.M.,
            Huang,G.Y., Chen,Z., Chen,M.D. and Chen,J.L.
  TITLE     Gene expression profiling in the human
            hypothalamus-pituitary-adrenal axis and full-length cDNA cloning
  JOURNAL   Proc Natl Acad Sci U S A 97 (17), 9543-9548 (2000)
   PUBMED   10931946
REFERENCE   9  (bases 1 to 4912)
  AUTHORS   Aguilera,M., Oliveros,M., Martinez-Padron,M., Barbas,J.A. and
            Ferrus,A.
  TITLE     Ariadne-1: a vital Drosophila gene is required in development and
            defines a new conserved family of ring-finger proteins
  JOURNAL   Genetics 155 (3), 1231-1244 (2000)
   PUBMED   10880484
REFERENCE   10 (bases 1 to 4912)
  AUTHORS   van der Reijden,B.A., Erpelinck-Verschueren,C.A., Lowenberg,B. and
            Jansen,J.H.
  TITLE     TRIADs: a new class of proteins with a novel cysteine-rich
            signature
  JOURNAL   Protein Sci 8 (7), 1557-1561 (1999)
   PUBMED   10422847
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG476221.1, BC000422.2,
            AC137630.9 and AL110187.1.
            
            On Jun 2, 2019 this sequence version replaced NM_006321.3.
            
            Summary: The protein encoded by this gene is an E3
            ubiquitin-protein ligase that polyubiquitinates some proteins,
            tagging them for degradation. The encoded protein upregulates p53
            in some cancer cells and may inhibit myelopoiesis. Several
            transcript variants encoding different isoforms have been found for
            this gene, although the full-length nature of some of them have not
            been determined yet. [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (2), as well as variants 1, 4, 5,
            and 6, encodes isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853565.20346.1,
                                           SRR11853567.20871.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356401.9/ ENSP00000348769.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                BG476221.1         25-42
            19-2150             BC000422.2         1-2132
            2151-2176           AC137630.9         32090-32115
            2177-4071           AL110187.1         1-1895
            4072-4912           AC137630.9         34011-34851
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..4912
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /note="ariadne RBR E3 ubiquitin protein ligase 2"
                     /db_xref="GeneID:10425"
                     /db_xref="HGNC:HGNC:690"
                     /db_xref="MIM:605615"
     exon            1..157
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            158..221
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            222..573
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     CDS             319..1800
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /EC_number="2.3.2.31"
                     /note="isoform a is encoded by transcript variant 2;
                     ariadne homolog 2; all-trans retinoic acid inducible RING
                     finger; protein ariadne-2 homolog; E3 ubiquitin-protein
                     ligase ARIH2; RING-type E3 ubiquitin transferase ARIH2"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase ARIH2 isoform a"
                     /protein_id="NP_006312.1"
                     /db_xref="CCDS:CCDS2780.1"
                     /db_xref="GeneID:10425"
                     /db_xref="HGNC:HGNC:690"
                     /db_xref="MIM:605615"
                     /translation="MSVDMNSQGSDSNEEDYDPNCEEEEEEEEDDPGDIEDYYVGVAS
                     DVEQQGADAFDPEEYQFTCLTYKESEGALNEHMTSLASVLKVSHSVAKLILVNFHWQV
                     SEILDRYKSNSAQLLVEARVQPNPSKHVPTSHPPHHCAVCMQFVRKENLLSLACQHQF
                     CRSCWEQHCSVLVKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKYRRYLFRDY
                     VESHYQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCATIRKW
                     LTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDFCWMCLGDWKTHG
                     SEYYECSRYKENPDIVNQSQQAQAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEK
                     IQERVMNNLGTWIDWQYLQNAAKLLAKCRYTLQYTYPYAYYMESGPRKKLFEYQQAQL
                     EAEIENLSWKVERADSYDRGDLENQMHIAEQRRRTLLKDFHDT"
     exon            574..641
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            642..705
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            706..856
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            857..978
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1088
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1206
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1257
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1258..1279
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1431
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1575
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1644
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..1728
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1729..4912
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4043..4048
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /note="hexamer: AATAAA"
     polyA_site      4071
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /note="major polyA site"
ORIGIN      
        1 ggagctgtgg ggacgactct tctggaggaa gcagcgcggg cttgaccggc gtcggcccgc
       61 cgcctccgct gccgcttcgc cccaatccgg tccctctggc ccggcctgac ccggtctggc
      121 ttgttcgggc tcagcggccg cgaggccgca gctcccgatg gaaatcatat tatgtagaat
      181 acttgggtga catctgcctg agagatctcc aagaattaca gaagacaaaa atactaatgc
      241 atttgagaaa gcggtagttt tggggggagg gggaaaaagc aactgctttc ctgatctgca
      301 acttggctgg atgctaagat gtcagtggac atgaatagcc aggggtctga cagcaatgaa
      361 gaggactatg acccaaattg tgaggaagag gaagaagaag aagaagacga ccctggggac
      421 atagaggact attacgtggg agtagccagc gatgtggagc agcagggggc tgatgccttt
      481 gatcccgagg agtaccagtt cacttgcttg acctacaagg aatctgaggg tgccctcaat
      541 gagcacatga ccagcttagc ttctgtccta aaggtatctc attcagttgc taaacttata
      601 ttagttaatt tccactggca agtttcagag atattggaca gatacaagtc caattctgct
      661 caactgcttg ttgaggctcg agttcagcct aatccatcaa aacatgttcc cacatcccat
      721 ccccctcacc actgtgcagt gtgtatgcag tttgtgcgaa aggaaaacct actctctctg
      781 gcctgtcagc accagttttg ccgcagctgc tgggagcagc actgctcagt tctcgtcaag
      841 gacggcgtgg gcgtgggagt ctcttgcatg gctcaggact gtccactccg tacaccagag
      901 gactttgtgt ttccattgct tcccaatgaa gaattgagag agaaatacag gcgctacctc
      961 ttcagggact atgtggagag tcattaccag ctccagctgt gccctggtgc agactgcccc
     1021 atggttattc gggtacagga gcctagagct cgccgagtac agtgcaatcg gtgcaacgag
     1081 gtcttctgtt tcaagtgtcg tcagatgtat cacgcaccca cagactgtgc cacaatccgg
     1141 aaatggctca cgaagtgtgc agacgactct gaaacagcca actacattag tgctcacact
     1201 aaagactgtc ccaagtgcaa catctgcatt gagaagaatg gaggctgcaa tcacatgcaa
     1261 tgctccaaat gtaaacacga cttctgctgg atgtgtctag gagattggaa gactcatggc
     1321 agtgaatact atgagtgcag tcgttacaag gagaatcctg acatcgtgaa ccagagccaa
     1381 caagcccagg cgagggaagc cctcaagaag tacttattct actttgagag gtgggaaaac
     1441 cacaataaaa gcttgcagct agaggcacag acataccagc ggattcacga gaagattcag
     1501 gagagggtca tgaacaatct ggggacatgg atcgactggc agtacctaca gaatgctgcc
     1561 aagctcttgg ccaagtgtcg atacaccctg caatacacct acccatatgc atattacatg
     1621 gagtccggac ccaggaagaa gctgtttgaa taccagcagg ctcagctgga ggctgagatc
     1681 gaaaacctct catggaaagt ggagcgtgca gacagctatg acagagggga cttggagaac
     1741 cagatgcata tagcggagca gcggaggaga accctgctga aagatttcca tgacacctaa
     1801 gttgggatgt ggatgtgccg gggtgaggaa gatgtggctg caaggtctcc cggctgccat
     1861 actgcatgct gcaggctctg cctttcatga ccccaggcaa cagccagggc cccactcctg
     1921 agagacactg gcaacacctc ttagttgatt tctgttttct tctcttttca ctttttgttt
     1981 ctaccagggt agaggccatg ttgaactggc ctcttttcag gacttttatt tccccctgga
     2041 tggttgttgg gagggaggga aagtgttttc tgaatggcta ttaatagtat tagatcatta
     2101 caacttatgt aactttcaaa ggttgtacaa ttatacaaaa aaaaaaaaaa ggcaaactat
     2161 aggataacac agagcccttt ttgaaaataa attggcattg gagtgtttta ccctctagct
     2221 gttttactta gaatgtaaca tatgctgcct acccacctca aaatgtctgt actgcaagag
     2281 ggccctgggc ctctgctttc catattcacg tttggccaga gttgtagtcc caaagaagag
     2341 catgggtggc agatggtagg gaattgaact ggcctgtgca atgggcatgg agcacaaggg
     2401 gtcacagcat gcctcctgcc ttaccgtggc agtacggaga cagtccagaa catggtcttc
     2461 ttgccacggg gtgttgttgt ctctggtggt gctgcatgtc tgtggctcac ctttattctt
     2521 gaaactgagg tttacctgga tctggctact gaggctagag cccacagcag aatggggttg
     2581 ggcctgtggc cccccaaact agggggtgtg ggttcatcac agtgttgcct tttgtctcct
     2641 aaagataggg atctactttt gaagggaatt gttcctccca aataaatttg ctttaccttg
     2701 gtcctttctt ttgtgccagt attcaagtgg tatagctctg agcagggtca catttggcca
     2761 aacctgacac tgtcttgctg cattctcctt tggcaaacat cagggtcaga attcaggata
     2821 gcccttccta gggcactgga ctttctggca tgggggctgt gtttgcacaa gttattttca
     2881 tgttacctgg agagtgtcca gaggctgctc tgaggctgag gtgtgttccc ccttgcctgg
     2941 ttccagctgt cagagggata ccatcctagg gtctgggaat ccaaggccac gagactcctt
     3001 ggtttgtggt ccgagatcct gtactaagga gggtctggcc agaggaacag accagctttt
     3061 gcacaatgaa gcgcaaggga acaagtggtt tgcctggtgt cctacctgtc ctgaacctgg
     3121 tcctgtgggc cattgaaaag ttagatctgt gatctctggg gtttttgtgg ctttgttcaa
     3181 tgcttccact ctagggcagg cagagcagtc tatactctcc caagcctgct tgacctccaa
     3241 gtagagctga tacagagatc tgtgaatatt gtgatagaaa ttctttggta ttcatacatt
     3301 tcagctgcaa gtcagcaatt tcccaggtac catgtaagct ataaaacagt cattcttaaa
     3361 gacagaggat agctgtgact catgggatca tgaggtccat ggctggttgc aggttccctt
     3421 tttccttcct caggttttgt ctcttcctgt gttgtcccca gcaagggaga gactgtgggg
     3481 tggattggga gaacagatta ggagtatagc aaatgaaccc agaatggaac agtggggagc
     3541 taactgtgaa tgaggagagt acctgctgca ggacctggag gtcaggtgtg aatgctgtat
     3601 tggcacaggg aataaatatc ctggcgtctg gagccttcac ctctccgtca agtccttcct
     3661 gtgatactgc catggcacag gatctgagtt gcagctctgc accctaaatc acaccctggg
     3721 cattgtctgg gctgcagggc tgccaggttc tgtacttgtg tccagctgtg gccctggatg
     3781 ctggagctgg agggttttct gtgctcagac tgtagcctgt agctcttggc ctgtgtagag
     3841 ccccctcctg tgccctcagt ggctgtcgtt tgttaacatc atcaggaaga tgggaaaggt
     3901 caggcagaat ttttctgccc tacaaagggt ggaagagaaa ggacacagta ttttcatgaa
     3961 tttaccatat atctttgttt ttcttcaacg aaaaagttaa ttgaggcaat gtcatctgct
     4021 caaagttgag tggtttattc acaataaact gtaagtttct gattataaaa atgtgtctag
     4081 agtctttctt catggggtct aggtagtctt ctgagcccac aggggtctgg ccagtgaaca
     4141 ccagaggagt gccttcctat cagtcagccc cttcttccag agggctgtgg aggccccagt
     4201 ggccaaactc ggctctgttt tccctttctg gctgttgggg gagagggcac ctgtaagtga
     4261 tccctgtgga atgaagtaga aaatcctgcc agttttccgc tctcctctcc ctcagtacag
     4321 gctgaatcat tccccaccac agatagatcc cagggttgtg gtagagtact gatataaatg
     4381 tcccaacccc cccattctga gacctccgga cccctccaaa gcagacatgg gaatgtaggg
     4441 ctgtggaata tggcagggtg aactccagct tcacgggagc cctaagggtt atgaagagac
     4501 aacttggggc agaaacaagt gtggagtcta atctcaaggg tgaaggacag gaaatgaggt
     4561 ttggattgat taagggggtc cacatttgtg gtgtcagaag tggcagtagt gtcagtgata
     4621 agtagctcag gggtagagtg gaggctccat agctgtgggc aacatgcaca gggcacctgt
     4681 ggctagaaga ggcctcatac attgccagtc tgaactccgc agctcttcct ctggacctca
     4741 ggtgtgccaa gccccacgct tggcacctgg gcatacagtg gtatgcaagt gattcacgtg
     4801 ctctagcttc caggagcttc caatccatgg ggaacataga aggaattgat cactagtgaa
     4861 tgtttgtttt ttgttttgag acagtctcgc tctatcgccc agactggagt ac
//
