LOCUS       NM_001349209            4970 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ariadne RBR E3 ubiquitin protein ligase 2 (ARIH2),
            transcript variant 4, mRNA.
ACCESSION   NM_001349209 XM_017005531
VERSION     NM_001349209.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Xu,P., Liu,Y., Liu,C., Guey,B., Li,L., Melenec,P., Ricci,J. and
            Ablasser,A.
  TITLE     The CRL5-SPSB3 ubiquitin ligase targets nuclear cGAS for
            degradation
  JOURNAL   Nature 627 (8005), 873-879 (2024)
   PUBMED   38418882
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Geng,S., Peng,W., Wang,X., Hu,X., Liang,H., Hou,J., Wang,F.,
            Zhao,G., Lu,M. and Cui,H.
  TITLE     ARIH2 regulates the proliferation, DNA damage and chemosensitivity
            of gastric cancer cells by reducing the stability of p21 via
            ubiquitination
  JOURNAL   Cell Death Dis 13 (6), 564 (2022)
   PUBMED   35732617
  REMARK    GeneRIF: ARIH2 regulates the proliferation, DNA damage and
            chemosensitivity of gastric cancer cells by reducing the stability
            of p21 via ubiquitination.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4970)
  AUTHORS   Kostrhon,S., Prabu,J.R., Baek,K., Horn-Ghetko,D., von Gronau,S.,
            Klugel,M., Basquin,J., Alpi,A.F. and Schulman,B.A.
  TITLE     CUL5-ARIH2 E3-E3 ubiquitin ligase structure reveals cullin-specific
            NEDD8 activation
  JOURNAL   Nat Chem Biol 17 (10), 1075-1083 (2021)
   PUBMED   34518685
REFERENCE   4  (bases 1 to 4970)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 4970)
  AUTHORS   Lumpkin,R.J., Ahmad,A.S., Blake,R., Condon,C.J. and Komives,E.A.
  TITLE     The Mechanism of NEDD8 Activation of CUL5 Ubiquitin E3 Ligases
  JOURNAL   Mol Cell Proteomics 20, 100019 (2021)
   PUBMED   33268465
REFERENCE   6  (bases 1 to 4970)
  AUTHORS   Marteijn,J.A., van der Meer,L.T., van Emst,L., van Reijmersdal,S.,
            Wissink,W., de Witte,T., Jansen,J.H. and Van der Reijden,B.A.
  TITLE     Gfi1 ubiquitination and proteasomal degradation is inhibited by the
            ubiquitin ligase Triad1
  JOURNAL   Blood 110 (9), 3128-3135 (2007)
   PUBMED   17646546
  REMARK    GeneRIF: The fine-tuning of Gfi1 protein levels regulated by Triad1
            defines an unexpected role for this protein in hematopoiesis.
REFERENCE   7  (bases 1 to 4970)
  AUTHORS   Marteijn,J.A., van Emst,L., Erpelinck-Verschueren,C.A.,
            Nikoloski,G., Menke,A., de Witte,T., Lowenberg,B., Jansen,J.H. and
            van der Reijden,B.A.
  TITLE     The E3 ubiquitin-protein ligase Triad1 inhibits clonogenic growth
            of primary myeloid progenitor cells
  JOURNAL   Blood 106 (13), 4114-4123 (2005)
   PUBMED   16118314
  REMARK    GeneRIF: proteasomal degradation of proteins that are ubiquitinated
            by Triad1 affects the clonogenic growth of primary myeloid
            progenitor cells
REFERENCE   8  (bases 1 to 4970)
  AUTHORS   Hu,R.M., Han,Z.G., Song,H.D., Peng,Y.D., Huang,Q.H., Ren,S.X.,
            Gu,Y.J., Huang,C.H., Li,Y.B., Jiang,C.L., Fu,G., Zhang,Q.H.,
            Gu,B.W., Dai,M., Mao,Y.F., Gao,G.F., Rong,R., Ye,M., Zhou,J.,
            Xu,S.H., Gu,J., Shi,J.X., Jin,W.R., Zhang,C.K., Wu,T.M.,
            Huang,G.Y., Chen,Z., Chen,M.D. and Chen,J.L.
  TITLE     Gene expression profiling in the human
            hypothalamus-pituitary-adrenal axis and full-length cDNA cloning
  JOURNAL   Proc Natl Acad Sci U S A 97 (17), 9543-9548 (2000)
   PUBMED   10931946
REFERENCE   9  (bases 1 to 4970)
  AUTHORS   Aguilera,M., Oliveros,M., Martinez-Padron,M., Barbas,J.A. and
            Ferrus,A.
  TITLE     Ariadne-1: a vital Drosophila gene is required in development and
            defines a new conserved family of ring-finger proteins
  JOURNAL   Genetics 155 (3), 1231-1244 (2000)
   PUBMED   10880484
REFERENCE   10 (bases 1 to 4970)
  AUTHORS   van der Reijden,B.A., Erpelinck-Verschueren,C.A., Lowenberg,B. and
            Jansen,J.H.
  TITLE     TRIADs: a new class of proteins with a novel cysteine-rich
            signature
  JOURNAL   Protein Sci 8 (7), 1557-1561 (1999)
   PUBMED   10422847
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC134028.10 and AC137630.9.
            
            On Jun 2, 2019 this sequence version replaced NM_001349209.1.
            
            Summary: The protein encoded by this gene is an E3
            ubiquitin-protein ligase that polyubiquitinates some proteins,
            tagging them for degradation. The encoded protein upregulates p53
            in some cancer cells and may inhibit myelopoiesis. Several
            transcript variants encoding different isoforms have been found for
            this gene, although the full-length nature of some of them have not
            been determined yet. [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (4), as well as variants 1, 2, 5,
            and 6, encodes isoform a.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.200096.1,
                                           SRR1803617.138202.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-157               AC134028.10        71341-71497
            158-279             AC134028.10        77217-77338
            280-631             AC134028.10        79961-80312
            632-699             AC137630.9         10081-10148
            700-763             AC137630.9         13388-13451
            764-914             AC137630.9         15594-15744
            915-1036            AC137630.9         17003-17124
            1037-1146           AC137630.9         19064-19173
            1147-1264           AC137630.9         22168-22285
            1265-1315           AC137630.9         23286-23336
            1316-1337           AC137630.9         23427-23448
            1338-1489           AC137630.9         27951-28102
            1490-1633           AC137630.9         28822-28965
            1634-1702           AC137630.9         30129-30197
            1703-1786           AC137630.9         31365-31448
            1787-4970           AC137630.9         31668-34851
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..4970
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /note="ariadne RBR E3 ubiquitin protein ligase 2"
                     /db_xref="GeneID:10425"
                     /db_xref="HGNC:HGNC:690"
                     /db_xref="MIM:605615"
     exon            1..157
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            158..279
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            280..631
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     CDS             377..1858
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /EC_number="2.3.2.31"
                     /note="isoform a is encoded by transcript variant 4;
                     ariadne homolog 2; all-trans retinoic acid inducible RING
                     finger; protein ariadne-2 homolog; E3 ubiquitin-protein
                     ligase ARIH2; RING-type E3 ubiquitin transferase ARIH2"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase ARIH2 isoform a"
                     /protein_id="NP_001336138.1"
                     /db_xref="CCDS:CCDS2780.1"
                     /db_xref="GeneID:10425"
                     /db_xref="HGNC:HGNC:690"
                     /db_xref="MIM:605615"
                     /translation="MSVDMNSQGSDSNEEDYDPNCEEEEEEEEDDPGDIEDYYVGVAS
                     DVEQQGADAFDPEEYQFTCLTYKESEGALNEHMTSLASVLKVSHSVAKLILVNFHWQV
                     SEILDRYKSNSAQLLVEARVQPNPSKHVPTSHPPHHCAVCMQFVRKENLLSLACQHQF
                     CRSCWEQHCSVLVKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKYRRYLFRDY
                     VESHYQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCATIRKW
                     LTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDFCWMCLGDWKTHG
                     SEYYECSRYKENPDIVNQSQQAQAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEK
                     IQERVMNNLGTWIDWQYLQNAAKLLAKCRYTLQYTYPYAYYMESGPRKKLFEYQQAQL
                     EAEIENLSWKVERADSYDRGDLENQMHIAEQRRRTLLKDFHDT"
     exon            632..699
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            700..763
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            764..914
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1036
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1146
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1264
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1315
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1337
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1489
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..1633
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1702
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1786
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1787..4970
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4101..4106
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /note="hexamer: AATAAA"
     polyA_site      4129
                     /gene="ARIH2"
                     /gene_synonym="ARI2; TRIAD1"
                     /note="major polyA site"
ORIGIN      
        1 ggagctgtgg ggacgactct tctggaggaa gcagcgcggg cttgaccggc gtcggcccgc
       61 cgcctccgct gccgcttcgc cccaatccgg tccctctggc ccggcctgac ccggtctggc
      121 ttgttcgggc tcagcggccg cgaggccgca gctcccgatt gagtctcgct ctgttgccca
      181 ggctggagtg cagtggtgca atctctgctc actgcaacct ccgtctcccg agttcaagca
      241 gttctcctgc ctcagcctcc tgagtagctg ggattacaga agacaaaaat actaatgcat
      301 ttgagaaagc ggtagttttg gggggagggg gaaaaagcaa ctgctttcct gatctgcaac
      361 ttggctggat gctaagatgt cagtggacat gaatagccag gggtctgaca gcaatgaaga
      421 ggactatgac ccaaattgtg aggaagagga agaagaagaa gaagacgacc ctggggacat
      481 agaggactat tacgtgggag tagccagcga tgtggagcag cagggggctg atgcctttga
      541 tcccgaggag taccagttca cttgcttgac ctacaaggaa tctgagggtg ccctcaatga
      601 gcacatgacc agcttagctt ctgtcctaaa ggtatctcat tcagttgcta aacttatatt
      661 agttaatttc cactggcaag tttcagagat attggacaga tacaagtcca attctgctca
      721 actgcttgtt gaggctcgag ttcagcctaa tccatcaaaa catgttccca catcccatcc
      781 ccctcaccac tgtgcagtgt gtatgcagtt tgtgcgaaag gaaaacctac tctctctggc
      841 ctgtcagcac cagttttgcc gcagctgctg ggagcagcac tgctcagttc tcgtcaagga
      901 cggcgtgggc gtgggagtct cttgcatggc tcaggactgt ccactccgta caccagagga
      961 ctttgtgttt ccattgcttc ccaatgaaga attgagagag aaatacaggc gctacctctt
     1021 cagggactat gtggagagtc attaccagct ccagctgtgc cctggtgcag actgccccat
     1081 ggttattcgg gtacaggagc ctagagctcg ccgagtacag tgcaatcggt gcaacgaggt
     1141 cttctgtttc aagtgtcgtc agatgtatca cgcacccaca gactgtgcca caatccggaa
     1201 atggctcacg aagtgtgcag acgactctga aacagccaac tacattagtg ctcacactaa
     1261 agactgtccc aagtgcaaca tctgcattga gaagaatgga ggctgcaatc acatgcaatg
     1321 ctccaaatgt aaacacgact tctgctggat gtgtctagga gattggaaga ctcatggcag
     1381 tgaatactat gagtgcagtc gttacaagga gaatcctgac atcgtgaacc agagccaaca
     1441 agcccaggcg agggaagccc tcaagaagta cttattctac tttgagaggt gggaaaacca
     1501 caataaaagc ttgcagctag aggcacagac ataccagcgg attcacgaga agattcagga
     1561 gagggtcatg aacaatctgg ggacatggat cgactggcag tacctacaga atgctgccaa
     1621 gctcttggcc aagtgtcgat acaccctgca atacacctac ccatatgcat attacatgga
     1681 gtccggaccc aggaagaagc tgtttgaata ccagcaggct cagctggagg ctgagatcga
     1741 aaacctctca tggaaagtgg agcgtgcaga cagctatgac agaggggact tggagaacca
     1801 gatgcatata gcggagcagc ggaggagaac cctgctgaaa gatttccatg acacctaagt
     1861 tgggatgtgg atgtgccggg gtgaggaaga tgtggctgca aggtctcccg gctgccatac
     1921 tgcatgctgc aggctctgcc tttcatgacc ccaggcaaca gccagggccc cactcctgag
     1981 agacactggc aacacctctt agttgatttc tgttttcttc tcttttcact ttttgtttct
     2041 accagggtag aggccatgtt gaactggcct cttttcagga cttttatttc cccctggatg
     2101 gttgttggga gggagggaaa gtgttttctg aatggctatt aatagtatta gatcattaca
     2161 acttatgtaa ctttcaaagg ttgtacaatt atacaaaaaa aaaaaaaagg caaactatag
     2221 gataacacag agcccttttt gaaaataaat tggcattgga gtgttttacc ctctagctgt
     2281 tttacttaga atgtaacata tgctgcctac ccacctcaaa atgtctgtac tgcaagaggg
     2341 ccctgggcct ctgctttcca tattcacgtt tggccagagt tgtagtccca aagaagagca
     2401 tgggtggcag atggtaggga attgaactgg cctgtgcaat gggcatggag cacaaggggt
     2461 cacagcatgc ctcctgcctt accgtggcag tacggagaca gtccagaaca tggtcttctt
     2521 gccacggggt gttgttgtct ctggtggtgc tgcatgtctg tggctcacct ttattcttga
     2581 aactgaggtt tacctggatc tggctactga ggctagagcc cacagcagaa tggggttggg
     2641 cctgtggccc cccaaactag ggggtgtggg ttcatcacag tgttgccttt tgtctcctaa
     2701 agatagggat ctacttttga agggaattgt tcctcccaaa taaatttgct ttaccttggt
     2761 cctttctttt gtgccagtat tcaagtggta tagctctgag cagggtcaca tttggccaaa
     2821 cctgacactg tcttgctgca ttctcctttg gcaaacatca gggtcagaat tcaggatagc
     2881 ccttcctagg gcactggact ttctggcatg ggggctgtgt ttgcacaagt tattttcatg
     2941 ttacctggag agtgtccaga ggctgctctg aggctgaggt gtgttccccc ttgcctggtt
     3001 ccagctgtca gagggatacc atcctagggt ctgggaatcc aaggccacga gactccttgg
     3061 tttgtggtcc gagatcctgt actaaggagg gtctggccag aggaacagac cagcttttgc
     3121 acaatgaagc gcaagggaac aagtggtttg cctggtgtcc tacctgtcct gaacctggtc
     3181 ctgtgggcca ttgaaaagtt agatctgtga tctctggggt ttttgtggct ttgttcaatg
     3241 cttccactct agggcaggca gagcagtcta tactctccca agcctgcttg acctccaagt
     3301 agagctgata cagagatctg tgaatattgt gatagaaatt ctttggtatt catacatttc
     3361 agctgcaagt cagcaatttc ccaggtacca tgtaagctat aaaacagtca ttcttaaaga
     3421 cagaggatag ctgtgactca tgggatcatg aggtccatgg ctggttgcag gttccctttt
     3481 tccttcctca ggttttgtct cttcctgtgt tgtccccagc aagggagaga ctgtggggtg
     3541 gattgggaga acagattagg agtatagcaa atgaacccag aatggaacag tggggagcta
     3601 actgtgaatg aggagagtac ctgctgcagg acctggaggt caggtgtgaa tgctgtattg
     3661 gcacagggaa taaatatcct ggcgtctgga gccttcacct ctccgtcaag tccttcctgt
     3721 gatactgcca tggcacagga tctgagttgc agctctgcac cctaaatcac accctgggca
     3781 ttgtctgggc tgcagggctg ccaggttctg tacttgtgtc cagctgtggc cctggatgct
     3841 ggagctggag ggttttctgt gctcagactg tagcctgtag ctcttggcct gtgtagagcc
     3901 ccctcctgtg ccctcagtgg ctgtcgtttg ttaacatcat caggaagatg ggaaaggtca
     3961 ggcagaattt ttctgcccta caaagggtgg aagagaaagg acacagtatt ttcatgaatt
     4021 taccatatat ctttgttttt cttcaacgaa aaagttaatt gaggcaatgt catctgctca
     4081 aagttgagtg gtttattcac aataaactgt aagtttctga ttataaaaat gtgtctagag
     4141 tctttcttca tggggtctag gtagtcttct gagcccacag gggtctggcc agtgaacacc
     4201 agaggagtgc cttcctatca gtcagcccct tcttccagag ggctgtggag gccccagtgg
     4261 ccaaactcgg ctctgttttc cctttctggc tgttggggga gagggcacct gtaagtgatc
     4321 cctgtggaat gaagtagaaa atcctgccag ttttccgctc tcctctccct cagtacaggc
     4381 tgaatcattc cccaccacag atagatccca gggttgtggt agagtactga tataaatgtc
     4441 ccaacccccc cattctgaga cctccggacc cctccaaagc agacatggga atgtagggct
     4501 gtggaatatg gcagggtgaa ctccagcttc acgggagccc taagggttat gaagagacaa
     4561 cttggggcag aaacaagtgt ggagtctaat ctcaagggtg aaggacagga aatgaggttt
     4621 ggattgatta agggggtcca catttgtggt gtcagaagtg gcagtagtgt cagtgataag
     4681 tagctcaggg gtagagtgga ggctccatag ctgtgggcaa catgcacagg gcacctgtgg
     4741 ctagaagagg cctcatacat tgccagtctg aactccgcag ctcttcctct ggacctcagg
     4801 tgtgccaagc cccacgcttg gcacctgggc atacagtggt atgcaagtga ttcacgtgct
     4861 ctagcttcca ggagcttcca atccatgggg aacatagaag gaattgatca ctagtgaatg
     4921 tttgtttttt gttttgagac agtctcgctc tatcgcccag actggagtac
//
