LOCUS       NM_001354046            5425 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens Rho guanine nucleotide exchange factor 7 (ARHGEF7),
            transcript variant 12, mRNA.
ACCESSION   NM_001354046
VERSION     NM_001354046.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Kawase,N., Sugihara,A., Kajiwara,K., Hiroshima,M., Akamatsu,K.,
            Nada,S., Matsumoto,K., Ueda,M. and Okada,M.
  TITLE     SRC kinase activator CDCP1 promotes hepatocyte growth
            factor-induced cell migration/invasion of a subset of breast cancer
            cells
  JOURNAL   J Biol Chem 298 (3), 101630 (2022)
   PUBMED   35085554
  REMARK    GeneRIF: SRC kinase activator CDCP1 promotes hepatocyte growth
            factor-induced cell migration/invasion of a subset of breast cancer
            cells.
REFERENCE   3  (bases 1 to 5425)
  AUTHORS   Keum,S., Yang,S.J., Park,E., Kang,T., Choi,J.H., Jeong,J.,
            Hwang,Y.E., Kim,J.W., Park,D. and Rhee,S.
  TITLE     Beta-Pix-dynamin 2 complex promotes colorectal cancer progression
            by facilitating membrane dynamics
  JOURNAL   Cell Oncol (Dordr) 44 (6), 1287-1305 (2021)
   PUBMED   34582006
  REMARK    GeneRIF: Beta-Pix-dynamin 2 complex promotes colorectal cancer
            progression by facilitating membrane dynamics.
REFERENCE   4  (bases 1 to 5425)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 5425)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 5425)
  AUTHORS   Turner,C.E., Brown,M.C., Perrotta,J.A., Riedy,M.C.,
            Nikolopoulos,S.N., McDonald,A.R., Bagrodia,S., Thomas,S. and
            Leventhal,P.S.
  TITLE     Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin
            repeat, ARF-GAP protein: A role in cytoskeletal remodeling
  JOURNAL   J Cell Biol 145 (4), 851-863 (1999)
   PUBMED   10330411
REFERENCE   7  (bases 1 to 5425)
  AUTHORS   Aghazadeh,B., Zhu,K., Kubiseski,T.J., Liu,G.A., Pawson,T., Zheng,Y.
            and Rosen,M.K.
  TITLE     Structure and mutagenesis of the Dbl homology domain
  JOURNAL   Nat Struct Biol 5 (12), 1098-1107 (1998)
   PUBMED   9846881
REFERENCE   8  (bases 1 to 5425)
  AUTHORS   Bagrodia,S., Taylor,S.J., Jordon,K.A., Van Aelst,L. and
            Cerione,R.A.
  TITLE     A novel regulator of p21-activated kinases
  JOURNAL   J Biol Chem 273 (37), 23633-23636 (1998)
   PUBMED   9726964
REFERENCE   9  (bases 1 to 5425)
  AUTHORS   Manser,E., Loo,T.H., Koh,C.G., Zhao,Z.S., Chen,X.Q., Tan,L.,
            Tan,I., Leung,T. and Lim,L.
  TITLE     PAK kinases are directly coupled to the PIX family of nucleotide
            exchange factors
  JOURNAL   Mol Cell 1 (2), 183-192 (1998)
   PUBMED   9659915
REFERENCE   10 (bases 1 to 5425)
  AUTHORS   Oh,W.K., Yoo,J.C., Jo,D., Song,Y.H., Kim,M.G. and Park,D.
  TITLE     Cloning of a SH3 domain-containing proline-rich protein, p85SPR,
            and its localization in focal adhesion
  JOURNAL   Biochem Biophys Res Commun 235 (3), 794-798 (1997)
   PUBMED   9207241
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL353704.10, AL390754.18 and
            AL139086.12.
            
            On Sep 23, 2020 this sequence version replaced NM_001354046.1.
            
            Summary: This gene encodes a protein that belongs to a family of
            cytoplasmic proteins that activate the Ras-like family of Rho
            proteins by exchanging bound GDP for GTP. It forms a complex with
            the small GTP binding protein Rac1 and recruits Rac1 to membrane
            ruffles and to focal adhesions. Multiple alternatively spliced
            transcript variants encoding different isoforms have been observed
            for this gene. [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (12) encodes the longest isoform
            (i).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.57737.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2145743
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000646102.2/ ENSP00000495631.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-382               AL353704.10        78163-78544
            383-469             AL353704.10        116758-116844
            470-554             AL390754.18        52725-52809
            555-685             AL390754.18        57308-57438
            686-887             AL390754.18        65115-65316
            888-976             AL139086.12        1906-1994
            977-1071            AL139086.12        12573-12667
            1072-1167           AL139086.12        12900-12995
            1168-1290           AL139086.12        36249-36371
            1291-1429           AL139086.12        42515-42653
            1430-1489           AL139086.12        43432-43491
            1490-1636           AL139086.12        44233-44379
            1637-1723           AL139086.12        46288-46374
            1724-1802           AL139086.12        48973-49051
            1803-1942           AL139086.12        49239-49378
            1943-2167           AL139086.12        51840-52064
            2168-2261           AL139086.12        54848-54941
            2262-2351           AL139086.12        57055-57144
            2352-2528           AL139086.12        60819-60995
            2529-2628           AL139086.12        69449-69548
            2629-2683           AL139086.12        70179-70233
            2684-5425           AL139086.12        71692-74433
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q34"
     gene            1..5425
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /note="Rho guanine nucleotide exchange factor 7"
                     /db_xref="GeneID:8874"
                     /db_xref="HGNC:HGNC:15607"
                     /db_xref="MIM:605477"
     exon            1..382
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     CDS             218..2806
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /note="isoform i is encoded by transcript variant 12; SH3
                     domain-containing proline-rich protein; PAK-interacting
                     exchange factor beta; Rho guanine nucleotide exchange
                     factor (GEF) 7"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 7 isoform
                     i"
                     /protein_id="NP_001340975.1"
                     /db_xref="CCDS:CCDS86360.1"
                     /db_xref="GeneID:8874"
                     /db_xref="HGNC:HGNC:15607"
                     /db_xref="MIM:605477"
                     /translation="MNSAEQTVTWLITLGVLESPKKTISDPEGFLQASLKDGVVLCRL
                     LERLLPGTIEKVYPEPRSESECLSNIREFLRGCGASLRLETFDANDLYQGQNFNKVLS
                     SLVTLNKVTADIGLGSDSVCARPSSHRIKSFDSLGSQSLHTRTSKLFQGQYRSLDMTD
                     NSNNQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTLNGRTGWFPSNYVR
                     EVKASEKPVSPKSGTLKSPPKGFDTTAINKSYYNVVLQNILETENEYSKELQTVLSTY
                     LRPLQTSEKLSSANISYLMGNLEEICSFQQMLVQSLEECTKLPEAQQRVGGCFLNLMP
                     QMKTLYLTYCANHPSAVNVLTEHSEELGEFMETKGASSPGILVLTTGLSKPFMRLDKY
                     PTLLKELERHMEDYHTDRQDIQKSMAAFKNLSAQCQEVRKRKELELQILTEAIRNWEG
                     DDIKTLGNVTYMSQVLIQCAGSEEKNERYLLLFPNVLLMLSASPRMSGFIYQGKLPTT
                     GMTITKLEDSENHRNAFEISGSMIERILVSCNNQQDLQEWVEHLQKQTKVTSVGNPTI
                     KPHSVPSHTLPSHPVTPSSKHADSKPAPLTPAYHTLPHPSHHGTPHTTINWGPLEPPK
                     TPKPWSLSCLRPAPPLRPSAALCYKEDLSKSPKTMKKLLPKRKPERKPSDEEFASRKS
                     TAALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTDLMHNHVLADDDQPSLDSLGRRSS
                     LSRLEPSDLSEDSDYDSIWTAHSYRMGSTSRSRKESAPQVLLPEEEKIIVEETKSNGQ
                     TVIEEKSLVDTVYALKDEVQELRQDNKKMKKSLEEEQRARKDLEKLVRKVLKNMNDPA
                     WDETNL"
     exon            383..469
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            470..554
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            555..685
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            686..887
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            888..976
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1071
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1167
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1290
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1429
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1489
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..1636
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            1637..1723
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            1724..1802
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..1942
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            1943..2167
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            2168..2261
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            2262..2351
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            2352..2528
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            2529..2628
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            2629..2683
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     exon            2684..5425
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5399..5404
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /note="hexamer: ATTAAA"
     polyA_site      5425
                     /gene="ARHGEF7"
                     /gene_synonym="BETA-PIX; COOL-1; COOL1; Nbla10314; P50;
                     P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB"
                     /note="major polyA site"
ORIGIN      
        1 gcaggttccc gagccgctcc tgagaaggcg cctgacagcg ggccggggcg cacggagaag
       61 cgggccgggc cggacctgct gggccgcgcc gagccaatcg ccggcgccgg ccgctcgatg
      121 ggcgaggcgg cggcggcggc ggcgggggcc gcgggccggg ccgccgctcc gaggtgaagg
      181 cgcgcgcccc tccccgcctg cctcccgggc cgcagcgatg aattccgccg agcaaaccgt
      241 tacgtggctc atcactctgg gggtgctgga gtcgcccaaa aaaaccatct cggacccgga
      301 gggctttctg caggcgtcgc tgaaggatgg ggtggtcctc tgcaggctgc tggagcgcct
      361 gctccccggg accatcgaga aagtctaccc cgagccccgg agcgagagcg agtgcctgag
      421 caacatccgc gagttcctgc gcggctgcgg ggcttccctg cggctggaga cgtttgatgc
      481 aaatgatttg tatcaggggc agaattttaa caaggtcctc agttccttag tgactctaaa
      541 taaagtaaca gcagacatcg ggctggggag tgactccgtg tgtgcccggc cctcgtctca
      601 ccgcataaag tcttttgact cccttggatc acagtctttg cacactcgga cttcaaaact
      661 gttccagggc cagtatcgga gtttggacat gaccgataat agcaacaatc aactggtagt
      721 aagagcaaag tttaacttcc agcagaccaa tgaggacgag ctttccttct caaaaggaga
      781 cgtcatccat gtcacccgtg tggaagaggg aggctggtgg gagggcacac tcaacggccg
      841 gaccggctgg ttccccagca actacgtgcg cgaggtcaag gccagcgaga agcctgtgtc
      901 tcccaaatca ggaacactga agagccctcc caaaggattt gatacgactg ccataaacaa
      961 aagctattac aatgtggtgc tacagaatat tttagaaaca gaaaatgaat attctaaaga
     1021 acttcagact gtgctttcaa cgtacctacg gccattgcag accagtgaga agttaagttc
     1081 agcaaacatt tcatatttaa tgggaaatct agaagaaata tgttctttcc agcaaatgct
     1141 cgtacagtct ttagaagaat gcaccaagtt gcccgaagct cagcagagag tcggaggctg
     1201 ctttttaaac ctgatgccac agatgaaaac cctgtacctc acgtattgtg ccaatcaccc
     1261 ttctgcagtg aatgtcctca cggaacacag tgaggagttg ggggagttca tggagaccaa
     1321 aggtgccagc agccctggga ttctcgtgct gaccacgggc ctgagcaaac ccttcatgcg
     1381 cctggataaa taccctacgc tgctcaaaga gctcgagaga cacatggagg attatcatac
     1441 agatagacaa gatattcaaa aatccatggc tgccttcaaa aacctttcag cccaatgtca
     1501 agaagtccgg aagaggaaag agcttgagct gcagatcctg acggaagcca tccggaactg
     1561 ggagggcgat gacattaaaa ctctgggcaa cgtcacttac atgtcccagg tcctgattca
     1621 gtgtgccgga agtgaggaaa agaatgaaag atatcttcta ctcttcccaa atgttttgct
     1681 aatgttgtct gccagtccta ggatgagtgg ctttatctat cagggaaagc ttccaacgac
     1741 aggaatgaca atcacaaagc ttgaggacag tgaaaatcat agaaatgcat ttgaaatatc
     1801 agggagcatg attgagcgga tattagtgtc gtgcaacaac cagcaggatc tgcaggaatg
     1861 ggtggagcac ctacagaagc aaacgaaggt cacgtctgtg ggaaacccca ccataaagcc
     1921 tcattcagtg ccatctcata ccctcccctc ccacccggtc actccgtcca gcaagcacgc
     1981 agacagcaag cccgcgccgc tgacgcccgc ctaccacacg ctgccccacc cctcccacca
     2041 cggcaccccg cacaccacca tcaactgggg acccctggag cctccgaaaa cacccaagcc
     2101 ctggagcctg agctgcctgc ggcccgcgcc tcccctccgg ccctcagctg ctctctgcta
     2161 caaggaggat cttagtaaga gccctaagac catgaaaaag ctgctgccca agcgcaaacc
     2221 tgaacggaag ccttcagatg aggagttcgc gtcccggaaa agcacagctg ctttggaaga
     2281 agatgctcag attctgaaag tcattgaagc ttactgcacc agcgccaaaa caaggcaaac
     2341 actcaattca acatggcaag gcactgacct gatgcataat cacgtcttgg ctgatgatga
     2401 ccaaccaagc ctagactccc tggggcgtcg cagtagcctt tctcgtttgg agccttcaga
     2461 cctctcggaa gactctgact atgacagtat atggacagcc catagttaca gaatgggttc
     2521 tacatctcgt tcacgcaaag aatctgctcc acaagttttg cttccagaag aagagaaaat
     2581 tatagtggaa gaaactaaaa gtaatggtca gacagtgata gaagaaaaga gtcttgtgga
     2641 taccgtatat gcattaaagg atgaagttca agaattaaga caggacaaca aaaagatgaa
     2701 gaaatctcta gaggaagaac agagagcccg caaagacctg gagaagctgg tgaggaaagt
     2761 cctgaagaac atgaatgatc ctgcctggga tgagaccaat ctataaggga tgtcctcagt
     2821 tctttctgtt gaagaccagt tctgaggtga agctgggcac ccctgaccca agtcggggtg
     2881 cactcaggac cacagggcag ggctgggtgg ggcgccacct tgctctctgt atatagaaaa
     2941 gctggagctt attctgcgaa tggagacgat caaaccatga ctgatgaatc cagacaggag
     3001 ggattgactc tgaggacctg agctacatca atccactctg tgaacatctc agttacctca
     3061 ttctgcaata agttcagtga ctgactaaaa gtcttgtttt tccagacttt gaattgaata
     3121 tataaatatt atatatacat gtttcttgta aatatcccat tttgaatgca tacctgtggt
     3181 ggttctgtcc gggctaatcc ccatgctaga atgtcctttc cagctacgtg aataagaagt
     3241 cccatgcccg catccaccgg aagcagaagc ctggtggatg cctggttcgt tccgcagcac
     3301 cagggcctcc accgtgctgt ggcagcaccc cccatgtcgg tatttctaaa taaccttatt
     3361 tatacctgca gagatacact tcagtcccat tcagaagtct tctcttaaag cagcattaca
     3421 gtcccagacc tgcgggtttc tgagggcaac ttgctggctg acagactcag tcttgacctc
     3481 aaggaaggcc catacggcac tgccgcatcc acctagaggt gtttgctctt gtccgctgtc
     3541 tgagtactgt gattctcaga tgagtttgct gcgttttggg aggacacaga cggttctgta
     3601 taggctagtt cagtaacaac aaaatacact gttttgtctt ccctcaaaga gagatcttac
     3661 tagaacctgt aaatagaatg tattatttat tataagtcac tgcagctgat gaaaacagat
     3721 ggaggccatg ctgcaggctg atactgatgg gtggagtttt gtcatcaggc cagcctcatc
     3781 ccgaggtctc ctccaccatt ggccgtagcc agcaggcttc agtgctcacc gaaagtaaaa
     3841 tcccctcctt cagcaagaat aaagcaatat acaccttagg ttccactaag taacataggc
     3901 ataagcaggg aacgtttccc ccactgtgtt ccagtgcaga ggagacgaag cctgtcctca
     3961 ccgcggctcg ctgggcccag gctggctctg gaaagcctgt gcggtcctgg gcaggaagcc
     4021 cggcccgtgg agcaggtttt cgttctgctt cagcaataaa taagggtgac cacagggact
     4081 ttgcttttgg tttcctttcc tgtgaaaagg ttggttttaa agtgagatac acttttccgt
     4141 agaacaagtg ttctatcttt aaaaacccaa attgcagcac cgtggattac tggtctcaga
     4201 acaactcatt gcgcatcaga tttgactctc tgattttctg tctattggcc aaattgccct
     4261 ttaactgcac ctgaatcctt tgtgtactga tgcctttgag ctgggcacct tgggagagtg
     4321 ttgtgttgct gtttacggtt cttccttgcc cttgctaatt acagtctctg gtgcccagca
     4381 agcccctttg gcttccttcc gtgactggtc acgttgtctg cctgggctca gcgtggacct
     4441 gccccatgct gcagaacctg gcctcacctg gacttttcac tagaattgcc agcttcctca
     4501 acttagcaga tcattcactc atgcgggcac aagcaaagat caacactttc ttttttggta
     4561 agcttgagtt ttacaagtta ttttttggtg atgcgtaaga cattgcagtg ggaaaccatt
     4621 caacttgagt ttattggagt ttgctgttgt agcaggtttt aactcaggaa caactcttgt
     4681 ctgatctctc cgcccctctg ccgggaggcg acattaactg tcctctcgga gccggtagcg
     4741 ttgctgtccg agtccccagg acggatctcc tgcagacctg ccttaatgct cagatcgaag
     4801 tatttcacaa gaatacttgt gtttttaaca gcccttcccc tggacggtgc ggccatgagg
     4861 gcctcatgtt acggcattgc cttttctttc tgtggatcca gtatcttcct cggcttttta
     4921 gggagcagga aaaatgcgtc tgagagcaac tctttttaaa aacctgccct gttgtatata
     4981 actgtgtctg tttcaccgtg tgacctccca agggggtggg aacttgatat aaacgtttaa
     5041 aggggccacg atttgcccga gggttactcc tttgctctca ccttgtatgg atgaggagat
     5101 gaagccattt cttatcctgt agatgtgaag cactttcagt tttcagcgat gttggaatgt
     5161 agcatcagaa gctcgttcct tcacactcag tggcgtctgt gcttgtccac atgcgctggg
     5221 cgtctgggac cttgaatgcc tgccctggtt gtgtggactc cttaatgcca atcatttctt
     5281 cacttctctg ggacacccag ggcgcctgtt gacaagtgtg gagaaactcc taatttaaat
     5341 gtcacagaca atgtcctagt gttgactact acaatgttga tgctacactg ttgtaattat
     5401 taaactgatt atttttctta tgtca
//
