LOCUS       NM_001367493            6735 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4),
            transcript variant 3, mRNA.
ACCESSION   NM_001367493 XM_005263681
VERSION     NM_001367493.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   He,X., Huang,N., Qiu,Y., Zhang,J., Liu,Y., Yin,X.L. and Lu,S.
  TITLE     Conformational Selection Mechanism Provides Structural Insights
            into the Optimization of APC-Asef Inhibitors
  JOURNAL   Molecules 26 (4), 962 (2021)
   PUBMED   33670371
  REMARK    GeneRIF: Conformational Selection Mechanism Provides Structural
            Insights into the Optimization of APC-Asef Inhibitors.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 6735)
  AUTHORS   Taniuchi,K., Furihata,M., Naganuma,S. and Saibara,T.
  TITLE     ARHGEF4 predicts poor prognosis and promotes cell invasion by
            influencing ERK1/2 and GSK-3alpha/beta signaling in pancreatic
            cancer
  JOURNAL   Int J Oncol 53 (5), 2224-2240 (2018)
   PUBMED   30226582
  REMARK    GeneRIF: Evaluation of an immunohistochemical staining of 102
            resected pancreatic cancer samples demonstrated that high ARHGEF4
            expression was correlated with an independent predictor of worse
            overall survival. ARHGEF4 induces the formation of cell protrusions
            by increasing phosphorylated ERK1/2 and GSK-3alpha/beta, resulting
            in an increase in motility and invasiveness in pancreatic ductal
            adenocarcinoma cells.
REFERENCE   4  (bases 1 to 6735)
  AUTHORS   Kim,S.B., Zhang,L., Yoon,J., Lee,J., Min,J., Li,W., Grishin,N.V.,
            Moon,Y.A., Wright,W.E. and Shay,J.W.
  TITLE     Truncated Adenomatous Polyposis Coli Mutation Induces
            Asef-Activated Golgi Fragmentation
  JOURNAL   Mol Cell Biol 38 (17), e00135-18 (2018)
   PUBMED   29866653
  REMARK    GeneRIF: The armadillo repeats in full-length APC interact with the
            APC residues 1362 to 1540 (APC-2,3 repeats), and this interaction
            competes off and inhibits Asef. Deletion of APC-2,3 repeats permits
            Asef interactions leading to downstream signaling events, including
            the induction of Golgi fragmentation through the activation of the
            Asef-ROCK-MLC2.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6735)
  AUTHORS   Antunez-Ortiz,D.L., Flores-Alfaro,E., Burguete-Garcia,A.I.,
            Bonnefond,A., Peralta-Romero,J., Froguel,P., Espinoza-Rojo,M. and
            Cruz,M.
  TITLE     Copy Number Variations in Candidate Genes and Intergenic Regions
            Affect Body Mass Index and Abdominal Obesity in Mexican Children
  JOURNAL   Biomed Res Int 2017, 2432957 (2017)
   PUBMED   28428959
  REMARK    GeneRIF: Our results indicate a possible contribution of CNVs in
            LEPR, NEGR1, ARHGEF4, and CPXCR1 and the intergenic regions 12q15c,
            15q21.1a, and 22q11.21d to the development of obesity, particularly
            abdominal obesity in Mexican children.
REFERENCE   6  (bases 1 to 6735)
  AUTHORS   Kawasaki,Y., Sato,R. and Akiyama,T.
  TITLE     Mutated APC and Asef are involved in the migration of colorectal
            tumour cells
  JOURNAL   Nat Cell Biol 5 (3), 211-215 (2003)
   PUBMED   12598901
REFERENCE   7  (bases 1 to 6735)
  AUTHORS   Salehi,A.H., Xanthoudakis,S. and Barker,P.A.
  TITLE     NRAGE, a p75 neurotrophin receptor-interacting protein, induces
            caspase activation and cell death through a JNK-dependent
            mitochondrial pathway
  JOURNAL   J Biol Chem 277 (50), 48043-48050 (2002)
   PUBMED   12376548
REFERENCE   8  (bases 1 to 6735)
  AUTHORS   Harrington,A.W., Kim,J.Y. and Yoon,S.O.
  TITLE     Activation of Rac GTPase by p75 is necessary for c-jun N-terminal
            kinase-mediated apoptosis
  JOURNAL   J Neurosci 22 (1), 156-166 (2002)
   PUBMED   11756498
REFERENCE   9  (bases 1 to 6735)
  AUTHORS   Kawasaki,Y., Senda,T., Ishidate,T., Koyama,R., Morishita,T.,
            Iwayama,Y., Higuchi,O. and Akiyama,T.
  TITLE     Asef, a link between the tumor suppressor APC and G-protein
            signaling
  JOURNAL   Science 289 (5482), 1194-1197 (2000)
   PUBMED   10947987
REFERENCE   10 (bases 1 to 6735)
  AUTHORS   Thiesen,S., Kubart,S., Ropers,H.H. and Nothwang,H.G.
  TITLE     Isolation of two novel human RhoGEFs, ARHGEF3 and ARHGEF4, in
            3p13-21 and 2q22
  JOURNAL   Biochem Biophys Res Commun 273 (1), 364-369 (2000)
   PUBMED   10873612
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC133785.2, AC112786.4,
            BC139742.1, AB042199.1 and AC009477.4.
            
            On Dec 4, 2018 this sequence version replaced XM_005263681.2.
            
            Summary: Rho GTPases play a fundamental role in numerous cellular
            processes that are initiated by extracellular stimuli that work
            through G protein coupled receptors. The protein encoded by this
            gene may form complex with G proteins and stimulate Rho-dependent
            signals. Multiple alternatively spliced transcript variants
            encoding different isoforms have been found, but the full-length
            nature of some variants has not been determined. [provided by
            RefSeq, Jun 2013].
            
            Transcript Variant: This variant (3) represents the longest
            transcript and encodes the longest isoform (c).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1968968,
                              SAMEA2144120 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000409359.7/ ENSP00000386794.3
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation, expression, longest
                                         protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-79                AC133785.2         3986-4064
            80-3058             AC112786.4         9337-12315
            3059-4025           BC139742.1         2-968
            4026-5671           AB042199.1         227-1872
            5672-6735           AC009477.4         22281-23344
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q21.1"
     gene            1..6735
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /note="Rho guanine nucleotide exchange factor 4"
                     /db_xref="GeneID:50649"
                     /db_xref="HGNC:HGNC:684"
                     /db_xref="MIM:605216"
     exon            1..79
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..5671
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /note="isoform c is encoded by transcript variant 3;
                     APC-stimulated guanine nucleotide exchange factor 1; Rho
                     guanine nucleotide exchange factor (GEF) 4"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 4 isoform
                     c"
                     /protein_id="NP_001354422.1"
                     /db_xref="CCDS:CCDS92866.1"
                     /db_xref="GeneID:50649"
                     /db_xref="HGNC:HGNC:684"
                     /db_xref="MIM:605216"
                     /translation="MLSVVHFLRSFFKTPEPGAHLPGEGEIEDNQLPTSPAEQVEQGW
                     NQQTDRDDSETLSQQSESGSDTKTDPFESASDTESLSGYLPRGVFHPLRGTPVDIEAP
                     WEYPDVSATGPPQEQHLTSVPGLHAKEELDLSPSLEDDSCKNGWRAFATVAGEQEAGH
                     LWDCATSLERESLLAGVPRHTGCCLQRATDSSGPEPVQGVAVQDLRGLSSVSLQKSRS
                     ESYLGIPVVWPFLLWCCELGRSWPHIHNRARALVLPSRGPLDNTAPLGTRTKKESTLG
                     PAGDTELLWSQPHSDVPCQPPLRTSCLLRTNRHHSAPETTGDKNRASPRLNCGHMRAL
                     VRAHSIAGFSPECPEDPVGQNVVKSGTHVKEGAKNERDPRIQNIPSPAPTQLSGPIPA
                     FQSGAPHLQGPCKPGGFRLQRASQDTPSAGLLGENQLRQDSRSCLVASCLTSELVKLS
                     AEEVPEPAECKSEQSPESRTQEPQGTQLAPRAADERETQKHLWGISVQAGNQTSNYTS
                     KYVLSEESKSPTRAKFPRQPSSEGTQVWSGDLMGCLEVSDSSDAPETTQKSSAIDTSK
                     AAEEAMVLDPNYREQALQGLSEFKAATVSHCGPGAEEGEQGPGGAGGRQLEPKAGGEA
                     SRGRGALIIVAVEQKGLQASRSNAGPVPETLPRDFPKERPESPLSTGETPCESPTRGK
                     TPAGNECELPAAPIQGAGDGALQRVAQAAELGRVLVPQAASEETPSTEEPPGERLRGE
                     SRSSGSGERGPEEAPEGGAAAARGQRPRVPALEPPQPPRGLRKGAQEPGKRPTFSKVT
                     SFRKGRPLATESPGGVPAPTTEGRRWGSSGPEGLPRENPPAAAGRDAPPLHHGDASAW
                     PEFVPQAAGDRTAGPAGAGHTGTSGDLGSRGPSSESCNAKRLKTTEKKLRARLALAHK
                     TFSNFIESIVLEKENTHERSPSSPKGEKEKSRLRQGSWRAFLKSKDAGSPKKPTLVSL
                     PLGPEVLSPAETDSHCEERAEDKEGYVFSDHWAPPLASTPLSSSLVSPEHRRKSEPTI
                     KCTATQEGGRYLPSGIFPEKSWLASPGSPRAQQAGIAHTLPSSSACCLAYENPGTPCR
                     PTSPKPLSPRPSAQRMGLHYPGRGSAISMVSLGSYSYVDSSSGDPERPKIPKGQTSFL
                     LSLQTLNQDEQKEESREGGQGPRGLGTVPWLRDLPGSENHMPWEEPAGEKPSCSHSQK
                     AFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLWLEKTQR
                     KKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMG
                     WPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELIS
                     DGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRL
                     RVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICE
                     GYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFL
                     EHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPV
                     QKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQ
                     SSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDV
                     LYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWL
                     KAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPAL
                     PSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK"
     exon            80..3592
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /inference="alignment:Splign:2.1.0"
     exon            3593..3898
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /inference="alignment:Splign:2.1.0"
     exon            3899..4025
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /inference="alignment:Splign:2.1.0"
     exon            4026..4165
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /inference="alignment:Splign:2.1.0"
     exon            4166..4345
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /inference="alignment:Splign:2.1.0"
     exon            4346..4522
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /inference="alignment:Splign:2.1.0"
     exon            4523..4702
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /inference="alignment:Splign:2.1.0"
     exon            4703..4935
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /inference="alignment:Splign:2.1.0"
     exon            4936..5065
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /inference="alignment:Splign:2.1.0"
     exon            5066..5197
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /inference="alignment:Splign:2.1.0"
     exon            5198..5441
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /inference="alignment:Splign:2.1.0"
     exon            5442..5519
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /inference="alignment:Splign:2.1.0"
     exon            5520..6735
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6716..6721
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /note="hexamer: AATAAA"
     polyA_site      6735
                     /gene="ARHGEF4"
                     /gene_synonym="ASEF; ASEF1; GEF4; SMIM39; STM6"
                     /note="major polyA site"
ORIGIN      
        1 gtagtgctgc ggccgggctc cgggcgtccc ggcggccacc atgctcagcg tcgtgcactt
       61 cctccggagc ttcttcaaga ctccagagcc aggtgcacac ctgccaggtg aaggtgagat
      121 tgaagataac cagctcccca catcccctgc agaacaagtg gagcagggat ggaaccagca
      181 aacagaccgc gatgattctg aaacgctgtc ccagcagagt gaaagtggat cagacacaaa
      241 aactgacccc tttgaaagtg cctctgacac agagtccttg tctgggtacc tcccgagggg
      301 agtcttccac cctctaagag gtactcccgt agatatagaa gcaccttggg aataccctga
      361 tgtctcagca actggacccc ctcaggagca gcatttgacc agtgttcctg ggcttcatgc
      421 aaaggaagaa ctcgatttgt cccctagctt agaggatgac tcttgcaaaa atgggtggcg
      481 agcctttgcc acagttgctg gagaacagga ggcaggacac ctctgggact gcgcgaccag
      541 cctggagcga gagtctttgc tggcaggggt tccccgacac acagggtgct gcttacagag
      601 ggccacagac agcagtggtc ctgagccagt acagggggtg gctgttcaag acctcagagg
      661 gctctccagt gtttctcttc agaaatccag gtctgagagc tatctgggca tcccagtggt
      721 ctggcccttc cttctctggt gctgtgaact gggtcggagt tggccacata tccacaacag
      781 ggccagggca ctggtgctac ctagcagagg cccactggac aacacagccc ctctggggac
      841 caggacaaag aaggaaagta ctctaggccc tgcaggggac acagaattgc tctggtccca
      901 gccccactcg gatgtcccct gccagcctcc tttgaggaca tcttgcctcc tacgcaccaa
      961 ccgtcaccat agtgccccag aaactactgg tgacaagaac agggcatccc ccagactcaa
     1021 ctgtgggcac atgagggcac tggtcagggc ccattccata gcagggtttt caccagagtg
     1081 ccccgaggat cccgtgggac agaatgttgt gaagtctggg acccatgtga aggaaggggc
     1141 caaaaatgaa cgagatccaa gaatacaaaa catcccttcc cctgcaccca cccagctgtc
     1201 tggcccgatt cctgcttttc agagtggggc tccccatctg cagggtccct gcaagcctgg
     1261 tgggtttcgc ttgcaaaggg cctctcagga cactccttct gcaggtctcc tgggggaaaa
     1321 ccagttaaga caggattcca ggtcatgtct ggtggcttca tgcctcacct cagagttagt
     1381 gaagctcagt gcagaggaag tgcctgagcc cgctgagtgc aagtcagagc aaagcccaga
     1441 aagcagaacc caggaacccc aaggcaccca actcgcacca agagctgctg atgagagaga
     1501 gacacagaag cacctctggg gcatttctgt ccaggcagga aaccaaacca gtaattacac
     1561 atcaaagtat gtgctcagcg aggaaagcaa gtcacctacc agggccaagt tcccacggca
     1621 gcctagcagt gaggggaccc aggtgtggag tggagacttg atgggctgct tggaagtgag
     1681 tgacagttca gatgcccctg agaccaccca gaaatcaagc gcaatagaca cttcaaaggc
     1741 agccgaagaa gccatggttc tagaccccaa ctacagggaa caggctttgc aaggtctttc
     1801 agaattcaag gcagccacgg tgtctcactg cggccccggg gctgaggagg gtgaacaggg
     1861 gcctgggggt gccgggggcc ggcagctgga gcccaaagca ggcggcgagg cctcgagggg
     1921 caggggcgcc ctcatcattg tagctgtgga gcagaaaggt cttcaggcca gcaggagcaa
     1981 tgcggggcct gttccagaaa cactgccccg tgactttcct aaggaaagac cagaatctcc
     2041 cttgagcact ggcgagaccc cctgtgagtc tcccactagg gggaaaacac cagccggtaa
     2101 tgagtgtgag ttgccagcag cccccataca gggagctggc gatggggctc ttcagcgggt
     2161 ggcccaggcc gcagagcttg ggagagtgct ggtcccccaa gctgcttcgg aagagacgcc
     2221 gagcacagag gagcccccgg gagagagact gcgtggggag agccggagct ccgggtcagg
     2281 ggagcgtggc ccggaggagg cccccgaagg cggtgctgca gcagcccggg gccagcgccc
     2341 ccgcgtcccc gccttggagc cgccccagcc gccacgcggg ctccgcaagg gcgcgcagga
     2401 gcctgggaag cgcccgacgt tttccaaggt gacctccttc aggaagggca ggcccttggc
     2461 cactgagagc ccaggagggg tcccggcccc gaccaccgag ggtcgccgct ggggctcttc
     2521 aggccccgag gggctcccca gggagaatcc gcccgctgcg gccggtcggg acgcaccgcc
     2581 tctgcaccac ggggacgcca gcgcctggcc cgagtttgtc ccgcaggctg caggcgacag
     2641 gactgcaggg ccggcaggag cggggcacac ggggacctca ggggatcttg gtagccgagg
     2701 gccttcctct gagagctgca acgcaaagag actcaaaaca acggagaaaa aactcagggc
     2761 aaggttggcc ttggctcata agaccttttc aaactttatt gagtcaatag ttctagagaa
     2821 agagaacacc catgaacgtt ccccaagttc tcccaagggc gagaaggaga agagcaggct
     2881 gcgccagggt tcctggcggg cgtttctgaa aagcaaagat gccggaagcc ccaaaaagcc
     2941 caccctagtg agtctgcctc taggacccga agttctctcc ccagcagaga ccgacagcca
     3001 ctgtgaggaa cgggcggagg acaaagaggg ctatgttttt agcgatcact gggcaccccc
     3061 acttgcctcc acacctttgt cctccagttt agtttctcca gaacacagga ggaaaagtga
     3121 accgaccatc aagtgcacag ccacccagga aggcggtagg tacctacctt caggtatctt
     3181 tccggaaaag tcctggctgg cgtcccccgg cagccctcgg gcccagcagg ctggaatcgc
     3241 acacaccctg ccttccagct ctgcctgctg cctggcatat gaaaaccccg ggacgccctg
     3301 cagacccacg agccccaagc ccctgagtcc caggcctagt gctcagcgga tgggtctcca
     3361 ctaccccggg aggggtagcg ccatctccat ggtttctctt ggaagctaca gctacgtgga
     3421 cagcagttca ggggaccctg aaagacccaa gattcccaag ggccagacca gtttcctgct
     3481 ttctctgcag acgctaaacc aagatgagca gaaggaagag agcagggaag gaggccaggg
     3541 tccgcgcggc ttgggcacag tgccctggct cagggacctt cctgggagtg agaaccacat
     3601 gccctgggaa gaaccagcag gtgagaagcc cagttgctct cacagtcaga aggcgttcca
     3661 catggagcct gcccagaagc cctgcttcac cactgacatg gtgacatggg ccctcctctg
     3721 catctctgca gagactgtgc gtggggaggc tccttcacag cctaggggca tccctcaccg
     3781 ctcgcccgtc agtgtggatg acctgtggct ggagaagaca cagagaaaga agttgcagaa
     3841 gcaggcccac gtcgaaagga ggctgcacat aggggcagtg cacaaagatg gagtcaagtg
     3901 ctggagaaag acgatcatta cctctccaga gtctttgaat ctccctagaa gaagccatcc
     3961 actctcccag agtgctccaa cgggactgaa ccacatgggc tggccagagc acacaccagg
     4021 cactgccatg cctgatggag ctctggacac agctgtctgc gctgacgaag tggggagcga
     4081 ggaggacctg tatgatgacc tgcacagctc cagccaccac tacagccacc ctggaggggg
     4141 tggggagcag ctggctatca atgagctcat cagcgatggc agtgtggtct gcgctgaagc
     4201 actctgggac catgtcacca tggacgacca ggagctgggc ttcaaagctg gggacgtcat
     4261 cgaagtgatg gatgccacca acagagagtg gtggtggggc cgggtcgccg atggcgaggg
     4321 ctggtttcca gccagcttcg ttcggctgag ggtgaatcag gacgagcccg cggatgacga
     4381 cgcccctctg gccgggaaca gcggagcgga ggacggcggg gcggaggcgc agagcagcaa
     4441 ggaccagatg cggaccaacg tcatcaacga gatcctcagc actgagcggg actacatcaa
     4501 gcacctgcgc gacatctgcg agggctacgt ccggcagtgc cgcaagcgcg cagacatgtt
     4561 cagcgaggag cagctgcgta ccatcttcgg gaacatcgag gacatctacc gctgccagaa
     4621 ggccttcgtg aaggccctgg agcagaggtt caaccgcgag cgcccacacc tgagcgagct
     4681 gggtgcctgc ttcctggagc atcaagccga cttccagatc tactcggagt actgcaataa
     4741 ccaccccaac gcctgcgtgg agctctcccg gctcaccaag ctcagcaagt acgtgtactt
     4801 cttcgaggcc tgccggctgc tgcagaagat gattgacatc tccctggatg gcttcctgct
     4861 gactccggtg cagaagatct gcaagtaccc tctgcagctg gccgagctgc tcaaatacac
     4921 gcacccccag cacagggact tcaaggatgt tgaagccgcc ttgcatgcca tgaagaacgt
     4981 ggcccagctc atcaacgagc ggaagcggag acttgagaac atcgacaaga ttgctcagtg
     5041 gcagagctcc atagaggact gggagggaga agatctcttg gtcaggagct cagaactcat
     5101 ctactcgggg gagctgactc gagttacaca gcctcaagcc aaaagccagc agcgaatgtt
     5161 ctttctcttt gaccaccagc tcatctactg taagaaggac ctgctccgcc gcgacgtgtt
     5221 gtactacaag ggccggctgg acatggacgg cctggaggtg gtggacctgg aggacgggaa
     5281 ggacagagac ctccatgtga gcatcaagaa cgccttccgg ctgcaccgtg gcgccacagg
     5341 ggacagccac ctgctgtgca ccaggaagcc cgagcagaag cagcgctggc tcaaggcctt
     5401 tgccagggag agggagcagg tgcagctgga ccaggagaca ggcttctcca tcactgaact
     5461 gcagaggaag caggccatgc tgaatgccag caagcagcag gtcacaggga agcccaaagc
     5521 tgttggccgg ccctgctacc tgacgcgcca gaagcaccca gccctgccca gcaaccggcc
     5581 ccagcagcag gtcctggtgc tggcggagcc caggcgcaag ccatctacct tctggcacag
     5641 catcagccgg ctggcaccct tccgcaagtg aactggtccc tgcctgacag cacctgctgg
     5701 gccttcctgc cagtggcccc cagtttttct tccccgaggc ccactcggcc tggccttcct
     5761 ctgcctgcaa gtgagcaggg atgggctggg gagttgcttg tgccaccaag acgtgccagg
     5821 tctgtactcc tgttgtcttt ttccctgctc ctggtgccct gaagagacca gcaagggggc
     5881 agaccccgca ctcgccacac cgccgctgca gcttgggccc catccgccct ctggacctgt
     5941 gtagggcctc actgctggag cggggaaacc gcagctcagc ccaggcccag ctggggagaa
     6001 ggcgctacct gcgtgggacc ctcttctctg gaaacctaat cctcctttca tttcctctgg
     6061 gcaggactct ctggccttct gtggcctgca atgccaggcc atgtgcccct ctgccctcta
     6121 gttctccaag tccccagccc ggccagtggt gccaggcagc ttgccacttg ggagggcaga
     6181 agccaggaat tccacaccct tgtgttgcgc ccggagcccg cccttcgcct cccagcccct
     6241 caagacaccg ctggctgctg gacaccctct tcacttgtgt gtgtgtgtgt agcggaaaag
     6301 gacaagacgg tgcagtcggc tgcatactcc cagtcgggag tgtggtcagt ctgcctgctg
     6361 ctgtgcggta gctccagaac cacctcgttc ctggttttgt ttggattttg gcatcttgtt
     6421 tttctaacaa caaacaatgg agaaaaagaa ttgattctta gtgacacaga agattgcctt
     6481 acgctcgtga gcgtgagaag ccataagaga gagaccgaat tctgtggctc agcacacagg
     6541 actgacccac agcccaggca gcgggtgtgt ggagatggcg ccctgtcctg ccaaggggcg
     6601 ccaggagcag agccagggcc tggcgagctg gcgtggagcc cacaggattc agcagcatgg
     6661 acagtcactc ttgcactatt ccttctccaa gccagaaacc acatttaatt tcataaataa
     6721 atttatgaaa agtaa
//
