LOCUS       NM_019555               3582 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens Rho guanine nucleotide exchange factor 3 (ARHGEF3),
            transcript variant 3, mRNA.
ACCESSION   NM_019555
VERSION     NM_019555.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Zhou,F., Ai,W., Zhang,Y., Hu,Q., Gan,M., Wang,J.B. and Han,T.
  TITLE     ARHGEF3 regulates the stability of ACLY to promote the
            proliferation of lung cancer
  JOURNAL   Cell Death Dis 13 (10), 870 (2022)
   PUBMED   36241648
  REMARK    GeneRIF: ARHGEF3 regulates the stability of ACLY to promote the
            proliferation of lung cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Gong,J., Tang,W., Lv,B., Zhang,S., Fan,T., Gao,G., Chen,D. and
            Liu,Y.
  TITLE     ARHGEF3 Associated with Invasion, Metastasis, and Proliferation in
            Human Osteosarcoma
  JOURNAL   Biomed Res Int 2021, 3381957 (2021)
   PUBMED   34350290
  REMARK    GeneRIF: ARHGEF3 Associated with Invasion, Metastasis, and
            Proliferation in Human Osteosarcoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3582)
  AUTHORS   Singh,N., Reyes-Ordonez,A., Compagnone,M.A., Moreno,J.F.,
            Leslie,B.J., Ha,T. and Chen,J.
  TITLE     Redefining the specificity of phosphoinositide-binding by human PH
            domain-containing proteins
  JOURNAL   Nat Commun 12 (1), 4339 (2021)
   PUBMED   34267198
  REMARK    GeneRIF: Redefining the specificity of phosphoinositide-binding by
            human PH domain-containing proteins.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3582)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 3582)
  AUTHORS   Wang,Y., Jiang,Q., Cai,H., Xu,Z., Wu,W., Gu,B., Li,L. and Cai,W.
  TITLE     Genetic variants in RET, ARHGEF3 and CTNNAL1, and relevant
            interaction networks, contribute to the risk of Hirschsprung
            disease
  JOURNAL   Aging (Albany NY) 12 (5), 4379-4393 (2020)
   PUBMED   32139661
  REMARK    GeneRIF: Genetic variants in RET, ARHGEF3 and CTNNAL1, and relevant
            interaction networks, contribute to the risk of Hirschsprung
            disease.
REFERENCE   6  (bases 1 to 3582)
  AUTHORS   Salehi,A.H., Xanthoudakis,S. and Barker,P.A.
  TITLE     NRAGE, a p75 neurotrophin receptor-interacting protein, induces
            caspase activation and cell death through a JNK-dependent
            mitochondrial pathway
  JOURNAL   J Biol Chem 277 (50), 48043-48050 (2002)
   PUBMED   12376548
REFERENCE   7  (bases 1 to 3582)
  AUTHORS   Arthur,W.T., Ellerbroek,S.M., Der,C.J., Burridge,K. and
            Wennerberg,K.
  TITLE     XPLN, a guanine nucleotide exchange factor for RhoA and RhoB, but
            not RhoC
  JOURNAL   J Biol Chem 277 (45), 42964-42972 (2002)
   PUBMED   12221096
  REMARK    GeneRIF: a guanine nucleotide exchange factor for RhoA and RhoB,
            but not RhoC
REFERENCE   8  (bases 1 to 3582)
  AUTHORS   Harrington,A.W., Kim,J.Y. and Yoon,S.O.
  TITLE     Activation of Rac GTPase by p75 is necessary for c-jun N-terminal
            kinase-mediated apoptosis
  JOURNAL   J Neurosci 22 (1), 156-166 (2002)
   PUBMED   11756498
REFERENCE   9  (bases 1 to 3582)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   10 (bases 1 to 3582)
  AUTHORS   Thiesen,S., Kubart,S., Ropers,H.H. and Nothwang,H.G.
  TITLE     Isolation of two novel human RhoGEFs, ARHGEF3 and ARHGEF4, in
            3p13-21 and 2q22
  JOURNAL   Biochem Biophys Res Commun 273 (1), 364-369 (2000)
   PUBMED   10873612
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA401228.1 and AL136832.1.
            
            On Jun 2, 2019 this sequence version replaced NM_019555.2.
            
            Summary: Rho-like GTPases are involved in a variety of cellular
            processes, and they are activated by binding GTP and inactivated by
            conversion of GTP to GDP by their intrinsic GTPase activity.
            Guanine nucleotide exchange factors (GEFs) accelerate the GTPase
            activity of Rho GTPases by catalyzing their release of bound GDP.
            This gene encodes a guanine nucleotide exchange factor, which
            specifically activates two members of the Rho GTPase family: RHOA
            and RHOB, both of which have a role in bone cell biology. It has
            been identified that genetic variation in this gene plays a role in
            the determination of bone mineral density (BMD), indicating the
            implication of this gene in postmenopausal osteoporosis.
            Alternatively spliced transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (3) lacks several 5' exons but has
            an alternate 5' exon, as compared to variant 1. The resulting
            isoform (3) has a distinct and shorter N-terminus, as compared to
            isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL136832.1, BC068513.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000296315.8/ ENSP00000296315.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-47                DA401228.1         19-65
            48-3582             AL136832.1         1-3535
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p14.3"
     gene            1..3582
                     /gene="ARHGEF3"
                     /gene_synonym="GEF3; STA3; XPLN"
                     /note="Rho guanine nucleotide exchange factor 3"
                     /db_xref="GeneID:50650"
                     /db_xref="HGNC:HGNC:683"
                     /db_xref="MIM:612115"
     exon            1..247
                     /gene="ARHGEF3"
                     /gene_synonym="GEF3; STA3; XPLN"
                     /inference="alignment:Splign:2.1.0"
     CDS             152..1732
                     /gene="ARHGEF3"
                     /gene_synonym="GEF3; STA3; XPLN"
                     /note="isoform 3 is encoded by transcript variant 3;
                     exchange factor found in platelets and leukemic and
                     neuronal tissues, XPLN; RhoGEF protein; 59.8 kDA protein;
                     Rho guanine nucleotide exchange factor (GEF) 3"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 3 isoform
                     3"
                     /protein_id="NP_062455.1"
                     /db_xref="CCDS:CCDS2878.1"
                     /db_xref="GeneID:50650"
                     /db_xref="HGNC:HGNC:683"
                     /db_xref="MIM:612115"
                     /translation="MVAKDYPFYLTVKRANCSLELPPASGPAKDAEEPSNKRVKPLSR
                     VTSLANLIPPVKATPLKRFSQTLQRSISFRSESRPDILAPRPWSRNAAPSSTKRRDSK
                     LWSETFDVCVNQMLTSKEIKRQEAIFELSQGEEDLIEDLKLAKKAYHDPMLKLSIMTE
                     QELNQIFGTLDSLIPLHEELLSQLRDVRKPDGSTEHVGPILVGWLPCLSSYDSYCSNQ
                     VAAKALLDHKKQDHRVQDFLQRCLESPFSRKLDLWNFLDIPRSRLVKYPLLLREILRH
                     TPNDNPDQQHLEEAINIIQGIVAEINTKTGESECRYYKERLLYLEEGQKDSLIDSSRV
                     LCCHGELKNNRGVKLHVFLFQEVLVITRAVTHNEQLCYQLYRQPIPVKDLLLEDLQDG
                     EVRLGGSLRGAFSNNERIKNFFRVSFKNGSQSQTHSLQANDTFNKQQWLNCIRQAKET
                     VLCAAGQAGVLDSEGSFLNPTTGSRELQGETKLEQMDQSDSESDCSMDTSEVSLDCER
                     MEQTDSSCGNSRHGESNV"
     exon            248..355
                     /gene="ARHGEF3"
                     /gene_synonym="GEF3; STA3; XPLN"
                     /inference="alignment:Splign:2.1.0"
     exon            356..526
                     /gene="ARHGEF3"
                     /gene_synonym="GEF3; STA3; XPLN"
                     /inference="alignment:Splign:2.1.0"
     exon            527..589
                     /gene="ARHGEF3"
                     /gene_synonym="GEF3; STA3; XPLN"
                     /inference="alignment:Splign:2.1.0"
     exon            590..686
                     /gene="ARHGEF3"
                     /gene_synonym="GEF3; STA3; XPLN"
                     /inference="alignment:Splign:2.1.0"
     exon            687..763
                     /gene="ARHGEF3"
                     /gene_synonym="GEF3; STA3; XPLN"
                     /inference="alignment:Splign:2.1.0"
     exon            764..1021
                     /gene="ARHGEF3"
                     /gene_synonym="GEF3; STA3; XPLN"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1192
                     /gene="ARHGEF3"
                     /gene_synonym="GEF3; STA3; XPLN"
                     /inference="alignment:Splign:2.1.0"
     exon            1193..1379
                     /gene="ARHGEF3"
                     /gene_synonym="GEF3; STA3; XPLN"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..3582
                     /gene="ARHGEF3"
                     /gene_synonym="GEF3; STA3; XPLN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3561..3566
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGEF3"
                     /gene_synonym="GEF3; STA3; XPLN"
                     /note="hexamer: AATAAA"
     polyA_site      3582
                     /gene="ARHGEF3"
                     /gene_synonym="GEF3; STA3; XPLN"
                     /note="major polyA site"
ORIGIN      
        1 agtcgagtcc tgcccggcta gaagccggct gtcggtctcc gtgtcgccgc cgccgcccgg
       61 catcgtggag ctggggcccc cttttgcctg ggagttttgt agtcgcctag ggtcagcggt
      121 gacatcccaa agggcaggcc cggcagccgc catggtggcc aaggattacc ccttctacct
      181 cacggtcaag agagcgaact gcagcctgga gctacccccg gccagcggtc cggccaagga
      241 cgctgaggag cctagtaata aacgggtcaa acccctttcc cgagtcacgt cgctagcaaa
      301 cctcatcccg cccgtgaagg ccacgccatt aaagcgcttc agtcaaaccc tgcagcgctc
      361 cattagcttc cgcagtgaga gccgccctga catcctcgcc ccccgaccct ggtccagaaa
      421 tgccgccccc tcgagcacga aacggagaga tagcaagctg tggagtgaga ccttcgatgt
      481 gtgcgtcaat cagatgctta catccaagga aatcaaacgt caggaggcga tctttgagct
      541 ttcccaagga gaagaagact tgatagaaga cttgaaatta gcaaaaaagg cctatcatga
      601 ccccatgctg aaactctcca taatgacaga acaagagttg aatcaaattt ttggaacact
      661 ggactctcta attcctctac atgaagagct ccttagtcag cttcgagatg ttaggaagcc
      721 tgatggctcg actgaacatg ttggtcccat cctcgtgggc tggctccctt gcctcagctc
      781 ctatgatagc tactgcagca atcaagtagc cgccaaagct ctgctggacc acaaaaagca
      841 agatcaccga gtccaggatt tcctacagcg atgtttagaa tcccccttta gccgcaaact
      901 agatctctgg aatttcctcg atattccaag aagccgcctg gtaaaatacc ctctgcttct
      961 ccgagaaatc ttgaggcaca caccaaatga taatccagat cagcagcact tggaagaagc
     1021 tataaatatc attcagggaa ttgtggcaga aatcaacacc aagactggtg aatctgaatg
     1081 ccgctattat aaagagcggc ttctttactt ggaagaaggc cagaaagact ccctgatcga
     1141 cagctctcga gtcttgtgtt gtcatggtga actgaagaac aatcggggcg tgaaactgca
     1201 tgttttcctg ttccaagaag tgcttgtgat cactcgagcc gtcacccaca atgagcagct
     1261 ttgctaccag ctgtaccgtc agccaatccc cgtgaaagac ctcctgctgg aagacctcca
     1321 ggatggagaa gtgaggctgg gtggctccct gcgaggggca ttcagcaaca atgagagaat
     1381 taaaaacttc ttcagagtca gtttcaaaaa tggatcccaa agtcagaccc actcgctaca
     1441 agccaatgac actttcaaca aacagcagtg gcttaactgt attcgtcaag ccaaagaaac
     1501 agttttgtgt gctgccgggc aagctggggt gcttgactcc gagggatcgt tcctaaatcc
     1561 caccaccggg agcagagagc tacagggaga aacaaaactt gagcagatgg accaatcgga
     1621 cagtgagtca gactgtagta tggacacgag tgaggtcagc ctcgactgtg agcgcatgga
     1681 acagacagac tcttcctgtg gaaacagcag gcacggtgaa agtaacgtct gacagaagca
     1741 tgtgcacttc gggaagcagg cctgcatctt acctgtacag tatttgcatt ccacagatgg
     1801 aacggtttgg agaagcactt tttcatactt ttgtgaaagt atacatgttg gcccagtctc
     1861 tcgtatctgt acctttgtcc ctagtactgt aactgccaat ctgtctgtgt aagctggaat
     1921 ctgtggcaac tattaccctg tgttgtattt cccaagtgtc tggatggatg gagaggtact
     1981 caaacaagtt actttcagtt gtcctgctgg attttaaaaa aatagaaaaa gaatctcaaa
     2041 actactgttt tacatagatt gtttgaagag tccttcctct tgtgcttctg taccactttc
     2101 ccagctctta gatgtggtag ctaaaggcac ggaatttaga cggccttgta aatagggcat
     2161 gaggaactca tctgtgtatt gggatggtat tagagagaga atcaggaaag accaactcat
     2221 gaagtgaact tggtttgatc ttactcaact agaaagcttg aaaacatccc tggggattct
     2281 gaaggcttaa ttttgcaaag gaggatgcat tgtctgaact ttgcaacttc atccagtgca
     2341 agtttgatgc aagaatgtat taggacataa aatagaggct gaccttaaaa gggccaggac
     2401 agaagcggct gccagctctg aatctttaac tgaaatgcac atggcaccag gaggtgtctc
     2461 tcatagttgg ttgctagcct aaaacatcag aatagaaccc aaagggctta ggaaggcctg
     2521 ccaggataac aagaaggccc tgtattcatt gtgtttcatc tgcctaggcc tactcattat
     2581 tttagagaat gaatgaagca acaaggaaga gagaccatga ctctatcgat gacactgttt
     2641 atagaaacac aggagaggaa gaatttggaa tgaaaagcac ttcgtcagaa ccttctgtgg
     2701 gagccattga gagaaaagca tggtccagtg ccttctgaga aaggccagag ctttgggctt
     2761 tcctgctctg cttttgggtc gtcaatttgc catctctggt tctgtgctat aatcagaatt
     2821 gtaattatgt tctccagagg ccaatttcat taactctgat taattagaat cagctagcca
     2881 gattagtaac ctctttgtcc agccttgatt tacagtgcag ggtaaagtgc agaccttaaa
     2941 aacagctaag tacctagaag agctccctgc aagtgtaaat attaaggatg acctgtgcaa
     3001 aattataccc acaccagcac tagtggtaat tattctaaat tattgccaaa aagttttttt
     3061 taatctgtct ttcaagttta cagaaaagaa agcagtaaat gcattgatgt cattttatta
     3121 tgtacatata tcatgtgcat tcaagctgtg tgacaagata tatcaatata aaaacaaggt
     3181 atatacttta ttattttttg aaaacaagga tattgtgatc aattttaccc tgtaaaacat
     3241 atttctgtat ttataggtct taaacatgat gaattttttc tattacaagt ttatttaaaa
     3301 ctgctttctc aagtcgttat tgatacagca agtgaacctg ctgcagacag aagcagagga
     3361 aagccaagaa cagcctttat tggtgaagaa aagaatgaat gattctttgt aggcgccatc
     3421 agccactttt agaagccatc agccagtgtg ttgggaaaag aggtttgtca agtgttggcc
     3481 tatgggaagg tggtcaatga atgttttgat gaaatgaatg tttttgtata atggccttaa
     3541 acttttctgg aagtatttca aataaattac attattaagt ca
//
