LOCUS       NM_001177693            6233 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28),
            transcript variant 2, mRNA.
ACCESSION   NM_001177693
VERSION     NM_001177693.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Choi,S.Y., Ahn,Y.R., Lee,E.B., Yu,M.J. and Lee,J.R.
  TITLE     Expression of a RhoA-Specific Guanine Nucleotide Exchange Factor,
            p190RhoGEF, in Mouse Macrophages Negatively Affects M1 Polarization
            and Inflammatory Responses
  JOURNAL   Front Immunol 13, 782475 (2022)
   PUBMED   35422804
  REMARK    GeneRIF: Expression of a RhoA-Specific Guanine Nucleotide Exchange
            Factor, p190RhoGEF, in Mouse Macrophages Negatively Affects M1
            Polarization and Inflammatory Responses.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 6233)
  AUTHORS   Di Gregorio,S.E., Volkening,K., Strong,M.J. and Duennwald,M.L.
  TITLE     Inclusion Formation and Toxicity of the ALS Protein RGNEF and Its
            Association with the Microtubule Network
  JOURNAL   Int J Mol Sci 21 (16), 5597 (2020)
   PUBMED   32764283
  REMARK    GeneRIF: Inclusion Formation and Toxicity of the ALS Protein RGNEF
            and Its Association with the Microtubule Network.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6233)
  AUTHORS   Yang,C., Li,Q., Chen,X., Zhang,Z., Mou,Z., Ye,F., Jin,S., Jun,X.,
            Tang,F. and Jiang,H.
  TITLE     Circular RNA circRGNEF promotes bladder cancer progression via
            miR-548/KIF2C axis regulation
  JOURNAL   Aging (Albany NY) 12 (8), 6865-6879 (2020)
   PUBMED   32305958
  REMARK    GeneRIF: Circular RNA circRGNEF promotes bladder cancer progression
            via miR-548/KIF2C axis regulation.
            Erratum:[Aging (Albany NY). 2023 May 31;15(10):4563-4565. doi:
            10.18632/aging.204791.. PMID: 37264529]
REFERENCE   5  (bases 1 to 6233)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 6233)
  AUTHORS   Yu,H.G., Nam,J.O., Miller,N.L., Tanjoni,I., Walsh,C., Shi,L.,
            Kim,L., Chen,X.L., Tomar,A., Lim,S.T. and Schlaepfer,D.D.
  TITLE     p190RhoGEF (Rgnef) promotes colon carcinoma tumor progression via
            interaction with focal adhesion kinase
  JOURNAL   Cancer Res 71 (2), 360-370 (2011)
   PUBMED   21224360
  REMARK    GeneRIF: Studies identify Rgnef as a novel regulator of colon
            carcinoma motility and invasion, and they show that a Rgnef-FAK
            linkage promotes colon carcinoma progression in vivo.
REFERENCE   7  (bases 1 to 6233)
  AUTHORS   Volkening,K., Leystra-Lantz,C. and Strong,M.J.
  TITLE     Human low molecular weight neurofilament (NFL) mRNA interacts with
            a predicted p190RhoGEF homologue (RGNEF) in humans
  JOURNAL   Amyotroph Lateral Scler 11 (1-2), 97-103 (2010)
   PUBMED   19488899
  REMARK    GeneRIF: RGNEF is a human homologue of p190RhoGEF, and its RNA is
            expressed in both brain and spinal cord
REFERENCE   8  (bases 1 to 6233)
  AUTHORS   Ohara,O., Nagase,T., Mitsui,G., Kohga,H., Kikuno,R., Hiraoka,S.,
            Takahashi,Y., Kitajima,S., Saga,Y. and Koseki,H.
  TITLE     Characterization of size-fractionated cDNA libraries generated by
            the in vitro recombination-assisted method
  JOURNAL   DNA Res 9 (2), 47-57 (2002)
   PUBMED   12056414
REFERENCE   9  (bases 1 to 6233)
  AUTHORS   van Horck,F.P., Ahmadian,M.R., Haeusler,L.C., Moolenaar,W.H. and
            Kranenburg,O.
  TITLE     Characterization of p190RhoGEF, a RhoA-specific guanine nucleotide
            exchange factor that interacts with microtubules
  JOURNAL   J Biol Chem 276 (7), 4948-4956 (2001)
   PUBMED   11058585
REFERENCE   10 (bases 1 to 6233)
  AUTHORS   Gebbink,M.F., Kranenburg,O., Poland,M., van Horck,F.P., Houssa,B.
            and Moolenaar,W.H.
  TITLE     Identification of a novel, putative Rho-specific GDP/GTP exchange
            factor and a RhoA-binding protein: control of neuronal morphology
  JOURNAL   J Cell Biol 137 (7), 1603-1613 (1997)
   PUBMED   9199174
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK094713.1, BC171850.1,
            AC093283.3 and CN478799.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 13, 2020 this sequence version replaced NM_001177693.1.
            
            Summary: This gene encodes a member of the Rho guanine nucleotide
            exchange factor family. The encoded protein interacts with low
            molecular weight neurofilament mRNA and may be involved in the
            formation of amyotrophic lateral sclerosis neurofilament
            aggregates. Alternate splicing results in multiple transcript
            variants.[provided by RefSeq, Apr 2010].
            
            Transcript Variant: This variant (2) lacks an in-frame exon in the
            coding region, compared to variant 1. The encoded isoform (2) is
            shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853558.19449.1,
                                           SRR11853567.24141.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142586
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000513042.7/ ENSP00000441436.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-127               AK094713.1         39-165
            128-5057            BC171850.1         12-4941
            5058-5058           AC093283.3         128274-128274
            5059-5826           BC171850.1         4943-5710
            5827-6233           CN478799.1         21-427              c
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q13.2"
     gene            1..6233
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /note="Rho guanine nucleotide exchange factor 28"
                     /db_xref="GeneID:64283"
                     /db_xref="HGNC:HGNC:30322"
                     /db_xref="MIM:612790"
     exon            1..127
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            128..171
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..5256
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /note="isoform 2 is encoded by transcript variant 2; Rho
                     interacting protein 2; rho-guanine nucleotide exchange
                     factor; 190 kDa guanine nucleotide exchange factor; Rho
                     guanine nucleotide exchange factor (GEF) 28"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 28
                     isoform 2"
                     /protein_id="NP_001171164.1"
                     /db_xref="CCDS:CCDS54870.1"
                     /db_xref="GeneID:64283"
                     /db_xref="HGNC:HGNC:30322"
                     /db_xref="MIM:612790"
                     /translation="MELSCSEAPLYGQMMIYAKFDKNVYLPEDAEFYFTYDGSHQRHV
                     MIAERIEDNVLQSSVPGHGLQETVTVSVCLCSEGYSPVTMGSGSVTYVDNMACRLARL
                     LVTQANRLTACSHQTLLTPFALTAGALPALDEELVLALTHLELPLEWTVLGSSSLEVS
                     SHRESLLHLAMRWGLAKLSQFFLCLPGGVQALALPNEEGATPLDLALREGHSKLVEDV
                     TNFQGRWSPSFSRVQLSEEASLHYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRA
                     FLVKAFEPEARPEERTAMPSSGAETEEEIKNSVSSRSAAEKEDIKRVKSLVVQHNEHE
                     DQHSLDLDRSFDILKKSKPPSTLLAAGRLSDMLNGGDEVYANCMVIDQVGDLDISYIN
                     IEGITATTSPESRGCTLWPQSSKHTLPTETSPSVYPLSENVEGTAHTEAQQSFMSPSS
                     SCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEGHSEPSHICYTPGSQSSSR
                     TGIPSGDELDSFETNTEPDFNISRAESLPLSSNLQSKESLLSGVRSRSYSCSSPKISL
                     GKTRLVRELTVCSSSEEQRAYSLSEPPRENRIQEEEWDKYIIPAKSESEKYKVSRTFS
                     FLMNRMTSPRNKSKTKSKDAKDKEKLNRHQFAPGTFSGVLQCLVCDKTLLGKESLQCS
                     NCNANVHKGCKDAAPACTKKFQEKYNKNKPQTILGNSSFRDIPQPGLSLHPSSSVPVG
                     LPTGRRETVGQVHPLSRSVPGTTLESFRRSATSLESESDHNSCRSRSHSDELLQSMGS
                     SPSTESFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFCNRQEKDVIKRQDVIFE
                     LMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKER
                     RQESCAGSDRNFVIDRIGDILVQQFSEENASKMKKIYGEFCCHHKEAVNLFKELQQNK
                     KFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHKDLRKA
                     LCLIKDMIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALMSEERTLL
                     YDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREV
                     ANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGGRTSESDED
                     KRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKP
                     DPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPG
                     SPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQN
                     LTRLLYSLQAALTIQDSHIEIHRLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEE
                     LANVHQLQHQLQQEQRRWLRRCEQQQRAQATRESWLQERERECQSQEELLLRSRGELD
                     LQLQEYQHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLPAVLLPGGPEVME
                     LNRSESLCHENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQV
                     DLKVDPSQPSNVSHKLWTAAGSGHQILPFHESSKDSCKNDLDTSHTESPTPHDSNSHR
                     PQLQAFITEAKLNLPTRTMTRQDGETGDGAKENIVYL"
     exon            172..319
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            320..613
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            614..797
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            798..978
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1048
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1101
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1162
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1284
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1565
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1773
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1774..1885
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..1928
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..2052
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2185
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2186..2241
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2242..2290
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2291..2435
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2436..2563
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2564..2704
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2705..2952
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2953..3075
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3076..3193
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3194..3448
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3449..3525
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3526..3704
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3705..3796
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3797..3979
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3980..4111
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4112..4212
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4213..4251
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4252..4299
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4300..4785
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4786..5086
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            5087..6233
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5413..5418
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /note="hexamer: ATTAAA"
     polyA_site      5431
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /note="major polyA site"
     regulatory      6214..6219
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
                     /note="hexamer: AATAAA"
     polyA_site      6233
                     /gene="ARHGEF28"
                     /gene_synonym="p190RHOGEF; RGNEF; RIP2"
ORIGIN      
        1 atcgccgggg agagccttcc ggactccggg cggcggctgg ctgggggcgc gttccgaggc
       61 gccctcaggc agggcgcggg ggcagagcct cgcctgggtt ctggaggctg cggggccatc
      121 gctccagatg cgaaagccat ggagttgagc tgcagcgaag cacctcttta cgggcagatg
      181 atgatctatg cgaagtttga caaaaatgtg tatcttcctg aagatgctga gttttacttt
      241 acttatgacg gatctcatca gcgacatgtc atgattgcag agcgcatcga ggataacgtt
      301 ctccagtcca gcgtcccagg ccatgggctt caggagacgg tgacggtatc tgtgtgcctc
      361 tgctcggaag gttactctcc ggtgaccatg ggctctggct cagtgaccta cgtggacaac
      421 atggcttgca ggctggctcg tctgctggtg acgcaggcca atcgcctcac agcctgcagc
      481 caccagaccc tgctgacccc atttgccttg acggcaggag cactgcctgc cttggatgag
      541 gagctcgtgc tggctctgac ccatctggaa ttgcctctag agtggactgt gttgggaagt
      601 tcttcacttg aagtatcttc tcacagagaa tctcttctac acctggctat gagatggggc
      661 ctggctaaac tttcccagtt cttcttgtgt ctcccggggg gagtccaggc cttggcttta
      721 cccaacgaag agggtgccac accattagac ttagctttac gtgaaggaca ctccaagctg
      781 gtggaagacg tcacaaattt tcagggcaga tggtccccaa gcttctcccg agtgcagctc
      841 agtgaagaag cctccttgca ttacattcac tcatcggaaa cgctgaccct gaccctgaac
      901 cacacagccg agcatttgtt ggaggcagat attaaactct tccggaaata cttttgggat
      961 agagcctttc ttgtcaaggc ctttgagcca gaagccaggc cagaggaaag aacagctatg
     1021 ccctccagcg gtgcagaaac tgaagaagag attaagaatt cagtgtccag cagatcagca
     1081 gctgaaaagg aagatataaa gcgtgtcaaa agcctggtgg ttcaacacaa tgaacatgaa
     1141 gaccagcaca gcctagattt ggatcgctcc ttcgatatcc taaaaaaatc caagccgccc
     1201 tcgacattgc ttgctgcagg ccggctttca gacatgctga atggaggtga tgaagtctac
     1261 gctaactgta tggtgattga tcaggttggt gatttggata tcagctatat taatatagag
     1321 ggaatcactg ccactaccag ccctgaatcc agaggttgca ctctgtggcc tcagagcagc
     1381 aaacacaccc ttcctacaga aaccagtccc agtgtgtacc cacttagtga aaatgtcgaa
     1441 gggacagcac acactgaagc ccagcagtcc ttcatgtcac catcaagttc gtgtgcttcc
     1501 aacttgaatc tttcttttgg ttggcatgga tttgaaaagg aacaaagtca tctaaagaaa
     1561 agaagttcta gccttgatgc cttggacgcc gacagtgaag gggaagggca ttctgagcca
     1621 tcccacatct gttacactcc agggtctcag agctcctcaa gaactgggat tcctagtggg
     1681 gatgaattgg actcttttga gactaacact gaaccggatt ttaatatctc cagggctgaa
     1741 tcccttcctc tatcaagtaa tctacagtcg aaggaatcac tgctttctgg agttcgctca
     1801 cgttcttatt cttgctcatc acccaaaatt tctttaggaa aaactcgttt ggtgcgtgaa
     1861 ttaacagtat gcagttcaag tgaagagcaa agagcttaca gcttatcgga gccaccaaga
     1921 gaaaacagaa ttcaggaaga agaatgggat aaatacatca tacctgccaa atcagagtct
     1981 gaaaaatata aagtgagtcg aactttcagt ttcctcatga ataggatgac tagccctcgg
     2041 aataaatcaa agacaaaaag caaggatgcc aaagataaag agaagctgaa tcgacatcag
     2101 tttgccccag gaacattctc tggggttctg cagtgtttgg tttgtgataa aacactcctg
     2161 gggaaagagt cactgcagtg ttctaactgt aatgcaaatg tgcacaaagg ttgtaaagat
     2221 gctgcgcctg catgcaccaa gaaattccaa gagaaatata acaagaacaa accacagacc
     2281 atccttggaa attcttcatt tagagacatc ccacagcctg gtctctcctt gcacccttct
     2341 tcctccgtgc ctgttggatt gccgactgga aggagggaga ctgtgggaca ggtccatcca
     2401 ttgtccagaa gtgttccagg caccaccttg gaaagcttca ggaggtcagc cacatccttg
     2461 gagtctgaga gtgaccataa cagctgcaga agcaggtctc attctgatga gctgctacag
     2521 tccatgggct cttctccctc tacagagtct ttcataatgg aagatgttgt ggattcttct
     2581 ctgtggagtg acctcagcag tgatgcccag gagtttgaag cagaatcttg gagtcttgtg
     2641 gtggatccct cattttgtaa taggcaggag aaggatgtca tcaaaagaca ggatgtcatt
     2701 tttgagctaa tgcaaacaga gatgcatcac atccagaccc tgttcatcat gtctgagatc
     2761 ttcaggaaag gcatgaaaga ggagctgcag ctggaccaca gcaccgtgga taaaattttc
     2821 ccctgtttag atgagttgct tgaaatccac aggcatttct tctacagtat gaaggaacga
     2881 aggcaggaat cctgtgctgg cagcgacagg aattttgtga tcgaccgaat tggagatatt
     2941 ttggtacaac agttttcaga agaaaatgca agtaaaatga agaaaatata tggagaattc
     3001 tgttgccatc ataaagaagc tgttaacctc tttaaagaac tccagcagaa taaaaagttt
     3061 cagaatttta ttaagctccg aaatagtaat cttttggctc gacgccgagg aattccagaa
     3121 tgcattctgt tggtcactca gcgtattaca aaataccctg tcttggtgga aaggatattg
     3181 cagtacacaa aggaaagaac tgaggaacat aaagacttac gcaaagcgct ttgcttaatt
     3241 aaagacatga ttgcaacagt ggatttaaaa gtcaatgaat atgagaaaaa ccaaaaatgg
     3301 cttgagatcc taaataagat tgaaaacaaa acatacacga agctcaaaaa tggacatgtg
     3361 tttaggaagc aggcactgat gagtgaagaa aggactctgt tatatgatgg ccttgtttac
     3421 tggaaaactg ctacaggtcg tttcaaagat atcctagctc tacttctaac tgatgtgctg
     3481 ctctttttac aagaaaaaga ccagaaatac atctttgcag ccgttgatca gaagccatca
     3541 gttatttccc ttcaaaagct tattgctaga gaagttgcta atgaggagag aggaatgttt
     3601 ctgatcagtg cttcatctgc tggtcctgag atgtatgaaa ttcacaccaa ttccaaggag
     3661 gaacgcaata actggatgag acggatccag caggctgtag aaagttgtcc tgaagaaaaa
     3721 gggggaagga caagtgaatc tgatgaagac aagaggaaag ctgaagccag agtggccaaa
     3781 attcagcaat gtcaagaaat actcactaac caagaccaac aaatttgtgc gtatttggag
     3841 gagaagctgc atatctatgc tgaacttgga gaactgagcg gatttgagga cgtccatcta
     3901 gagccccacc tccttattaa acctgaccca ggcgagcctc cccaggcagc ctcattactg
     3961 gcagcagcac tgaaagaagc tgagagccta caagttgcag tgaaggcctc acagatgggc
     4021 gccgtgagtc aatcatgtga ggacagttgt ggagactctg tcttggcgga cacactcagt
     4081 tctcatgatg taccaggatc accgactgcc tcattagtca caggagggag agaaggaaga
     4141 ggctgttcgg atgtggatcc cgggatccag ggtgtggtaa ccgacttggc cgtctctgat
     4201 gcaggggaga aggtggaatg tagaaatttt ccaggttctt cacaatcaga gattatacaa
     4261 gccatacaga atttaacccg tctcttatac agccttcagg ccgccttgac cattcaggac
     4321 agccacattg agatccacag gctggttctc cagcagcagg agggcctgtc tctcggccac
     4381 tctatcctcc gaggcggccc cttgcaggac cagaagtctc gcgacgcgga caggcagcat
     4441 gaggagctgg ccaatgtgca ccagcttcag caccagctcc agcaggagca gcggcgctgg
     4501 ctgcgcaggt gtgagcagca gcagcgggcg caggcgacca gggagagctg gctgcaggag
     4561 cgggagcggg agtgccagtc gcaggaggag ctgctgctgc ggagccgggg cgagctggac
     4621 ctccagctcc aggagtacca gcacagcctg gagcggctga gggagggcca gcgcctggtg
     4681 gagagggagc aggcgaggat gcgggcccag cagagcctgc tgggccactg gaagcacggc
     4741 cggcagagga gcctgcccgc ggtgctcctt ccgggtggcc ccgaggtaat ggaacttaat
     4801 cgatctgaga gtttatgtca tgaaaactca ttcttcatca atgaagcttt agtacaaatg
     4861 tcatttaaca ctttcaacaa actgaatcca tcagttatcc atcaggatgc cacttaccct
     4921 acaactcaat ctcattctga cttggtgagg actagtgaac atcaagtaga cctcaaggtg
     4981 gacccttctc agccttcgaa tgtcagtcac aaactgtgga cagccgctgg ttccggccat
     5041 cagatacttc ctttccatga aagcagcaag gattcttgta aaaatgattt ggacacctcc
     5101 cacactgagt ccccaacccc ccatgactca aattcacacc gccctcaact gcaggcgttt
     5161 ataacagaag caaagctaaa tctaccgaca aggacaatga ccagacaaga tggggaaact
     5221 ggagatggag ccaaagaaaa tattgtttac ctctaattgt gttgtcattt ttccaaacaa
     5281 aacaaaacac tggcactttt gggagaaact ttttgtctcc attccttatg tatgtgtgat
     5341 tgtctgtgtc caaattgctt taagaataat atttaatatt tcctggaagc tcattttttt
     5401 ggcatgagtc taattaaatt attgaaagcc accctgtttg tataatcttt aacttatcaa
     5461 atctaatttc agatttctgg aggagaaact aacttgaata agcaggacta ttttaaaagt
     5521 tgttttgacg ctagagtaaa attccatgtc acattttcta cccaatcatc tggatttcaa
     5581 gattcctttt aagatctcaa tgaagcaatt tggatttaaa gagtggtatt cacaaggggt
     5641 gaactttcac agtcagggca gttgcctcag tgcccacata ggcagaggag gatgtgggaa
     5701 agggtttttc tcagctagtt tttgtgtgct catttcttct gggagcatta aaagtggtga
     5761 tctgttacag tcactattca actgggcacg tgttgtgatt ggtcagtcac tgagccaggg
     5821 atacagtccg gacttgctta gtacctaagc ctaatgctgg tggggtttca agacatggtt
     5881 cagcatcatc ttttaacaag gcccagaggc ccagagcccc catcaagtca ttttgatgta
     5941 aatagtgaac tttgttagag ccctcacttc tatcaatcag ctgtcctgtc cctgccagca
     6001 cctggagcac caactaccac tccctggaaa gaacccttcc ctgcagtttt ttaaggacaa
     6061 aactgcccac tcctcattaa gtttgctgcc tggatacact tttccacaaa ggaaaactgg
     6121 catatcctgc cttccgagta gtatgggtct ctgtgtgaga aaccaggaga tattttcatc
     6181 ttgttcggaa atacttgtat gtattttggt gtcaataaat atcttgtacc tca
//
