LOCUS       NM_182947               2554 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens Rho guanine nucleotide exchange factor 25 (ARHGEF25),
            transcript variant 1, mRNA.
ACCESSION   NM_182947
VERSION     NM_182947.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Meng,L., Shang,H., Liu,Q., Li,Z., Wang,X., Li,Q., Li,F., Zhao,Z.
            and Liu,C.
  TITLE     Lnc-PSMA8-1 activated by GEFT promotes rhabdomyosarcoma progression
            via upregulation of mTOR expression by sponging miR-144-3p
  JOURNAL   BMC Cancer 24 (1), 79 (2024)
   PUBMED   38225540
  REMARK    GeneRIF: Lnc-PSMA8-1 activated by GEFT promotes rhabdomyosarcoma
            progression via upregulation of mTOR expression by sponging
            miR-144-3p.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Wang,X., Zhang,X., Sun,J., Sun,Y., Zhang,Y., He,L., Wang,P., Li,F.
            and Sun,C.
  TITLE     Guanine nucleotide exchange factor T exerts the cancer-promoting
            function in cholangiocarcinoma by enhancing the
            Wnt-GSK-3beta-beta-catenin cascade via regulation of Rac1/Cdc42
  JOURNAL   Toxicol Appl Pharmacol 467, 116492 (2023)
   PUBMED   36977438
  REMARK    GeneRIF: Guanine nucleotide exchange factor T exerts the
            cancer-promoting function in cholangiocarcinoma by enhancing the
            Wnt-GSK-3beta-beta-catenin cascade via regulation of Rac1/Cdc42.
REFERENCE   3  (bases 1 to 2554)
  AUTHORS   van Unen,J., Yin,T., Wu,Y.I., Mastop,M., Gadella,T.W. Jr. and
            Goedhart,J.
  TITLE     Kinetics of recruitment and allosteric activation of ARHGEF25
            isoforms by the heterotrimeric G-protein Galphaq
  JOURNAL   Sci Rep 6, 36825 (2016)
   PUBMED   27833100
  REMARK    GeneRIF: p63RhoGEF(619) relocates to the plasma membrane upon
            activation of Galphaq coupled GPCRs, resembling the well-known
            activation mechanism of RhoGEFs activated by Galpha12/13.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2554)
  AUTHORS   van Unen,J., Reinhard,N.R., Yin,T., Wu,Y.I., Postma,M.,
            Gadella,T.W. and Goedhart,J.
  TITLE     Plasma membrane restricted RhoGEF activity is sufficient for
            RhoA-mediated actin polymerization
  JOURNAL   Sci Rep 5, 14693 (2015)
   PUBMED   26435194
  REMARK    GeneRIF: p63RhoGEF can rapidly activate RhoA through endogenous
            G-protein coupled receptors.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2554)
  AUTHORS   Sun,C., Liu,C., Li,S., Li,H., Wang,Y., Xie,Y., Li,B., Cui,X.,
            Chen,Y., Zhang,W. and Li,F.
  TITLE     Overexpression of GEFT, a Rho family guanine nucleotide exchange
            factor, predicts poor prognosis in patients with rhabdomyosarcoma
  JOURNAL   Int J Clin Exp Pathol 7 (4), 1606-1615 (2014)
   PUBMED   24817957
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2554)
  AUTHORS   Lutz,S., Freichel-Blomquist,A., Yang,Y., Rumenapp,U., Jakobs,K.H.,
            Schmidt,M. and Wieland,T.
  TITLE     The guanine nucleotide exchange factor p63RhoGEF, a specific link
            between Gq/11-coupled receptor signaling and RhoA
  JOURNAL   J Biol Chem 280 (12), 11134-11139 (2005)
   PUBMED   15632174
  REMARK    GeneRIF: a new signaling pathway by which G alpha(q/11)-coupled
            receptors specifically induce Rho signaling through a direct
            interaction of activated G alpha(q/11) subunits with p63RhoGEF.
REFERENCE   7  (bases 1 to 2554)
  AUTHORS   Bryan,B., Kumar,V., Stafford,L.J., Cai,Y., Wu,G. and Liu,M.
  TITLE     GEFT, a Rho family guanine nucleotide exchange factor, regulates
            neurite outgrowth and dendritic spine formation
  JOURNAL   J Biol Chem 279 (44), 45824-45832 (2004)
   PUBMED   15322108
  REMARK    GeneRIF: GEFT enhances dendritic spine formation and neurite
            outgrowth in primary neurons and neuroblastoma cells, respectively,
            through the activation of Rac/Cdc42-PAK signaling pathways.
REFERENCE   8  (bases 1 to 2554)
  AUTHORS   Lutz,S., Freichel-Blomquist,A., Rumenapp,U., Schmidt,M.,
            Jakobs,K.H. and Wieland,T.
  TITLE     p63RhoGEF and GEFT are Rho-specific guanine nucleotide exchange
            factors encoded by the same gene
  JOURNAL   Naunyn Schmiedebergs Arch Pharmacol 369 (5), 540-546 (2004)
   PUBMED   15069594
REFERENCE   9  (bases 1 to 2554)
  AUTHORS   Guo,X., Stafford,L.J., Bryan,B., Xia,C., Ma,W., Wu,X., Liu,D.,
            Songyang,Z. and Liu,M.
  TITLE     A Rac/Cdc42-specific exchange factor, GEFT, induces cell
            proliferation, transformation, and migration
  JOURNAL   J Biol Chem 278 (15), 13207-13215 (2003)
   PUBMED   12547822
REFERENCE   10 (bases 1 to 2554)
  AUTHORS   Souchet,M., Portales-Casamar,E., Mazurais,D., Schmidt,S., Leger,I.,
            Javre,J.L., Robert,P., Berrebi-Bertrand,I., Bril,A., Gout,B.,
            Debant,A. and Calmels,T.P.
  TITLE     Human p63RhoGEF, a novel RhoA-specific guanine nucleotide exchange
            factor, is localized in cardiac sarcomere
  JOURNAL   J Cell Sci 115 (Pt 3), 629-640 (2002)
   PUBMED   11861769
  REMARK    GeneRIF: Results show that p63RhoGEF is a RhoA-specific GEF that
            may play a key role in actin cytoskeleton reorganization in
            different tissues, especially in heart cellular morphology.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC025165.27.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_182947.3.
            
            Summary: Rho GTPases alternate between an inactive GDP-bound state
            and an active GTP-bound state, and GEFs facilitate GDP/GTP
            exchange. This gene encodes a guanine nucleotide exchange factor
            (GEF) which interacts with Rho GTPases involved in contraction of
            vascular smooth muscles, regulation of responses to angiotensin II
            and lens cell differentiation. Multiple transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jan 2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript (1) and encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC047559.1, SRR1660803.259832.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000286494.9/ ENSP00000286494.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-557               AC025165.27        5354-5910
            558-772             AC025165.27        6849-7063
            773-868             AC025165.27        7183-7278
            869-945             AC025165.27        7359-7435
            946-1012            AC025165.27        7613-7679
            1013-1116           AC025165.27        7935-8038
            1117-1186           AC025165.27        8250-8319
            1187-1276           AC025165.27        8435-8524
            1277-1369           AC025165.27        8608-8700
            1370-1420           AC025165.27        8886-8936
            1421-1498           AC025165.27        9156-9233
            1499-1699           AC025165.27        9431-9631
            1700-1880           AC025165.27        9756-9936
            1881-2092           AC025165.27        10203-10414
            2093-2554           AC025165.27        10703-11164
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.3"
     gene            1..2554
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /note="Rho guanine nucleotide exchange factor 25"
                     /db_xref="GeneID:115557"
                     /db_xref="HGNC:HGNC:30275"
                     /db_xref="MIM:610215"
     exon            1..557
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     CDS             461..2203
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /note="isoform 1 is encoded by transcript variant 1;
                     RAC/CDC42 exchange factor; guanine nucleotide exchange
                     factor GEFT; RhoA/RAC/CDC42 exchange factor; rac/Cdc42/Rho
                     exchange factor GEFT; rhoA/Rac/Cdc42 guanine nucleotide
                     exchange factor GEFT; Rho guanine nucleotide exchange
                     factor (GEF) 25"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 25
                     isoform 1"
                     /protein_id="NP_891992.3"
                     /db_xref="CCDS:CCDS8947.1"
                     /db_xref="GeneID:115557"
                     /db_xref="HGNC:HGNC:30275"
                     /db_xref="MIM:610215"
                     /translation="MRGGHKGGRCACPRVIRKVLAKCGCCFARGGRESYSIAGSEGSI
                     SASAASGLAAPSGPSSGLSSGPCSPGPPGPVSGLRRWLDHSKHCLSVETEADSGQAGP
                     YENWMLEPALATGEELPELTLLTTLLEGPGDKTQPPEEETLSQAPESEEEQKKKALER
                     SMYVLSELVETEKMYVDDLGQIVEGYMATMAAQGVPESLRGRDRIVFGNIQQIYEWHR
                     DYFLQELQRCLKDPDWLAQLFIKHERRLHMYVVYCQNKPKSEHVVSEFGDSYFEELRQ
                     QLGHRLQLNDLLIKPVQRIMKYQLLLKDFLKYYNRAGMDTADLEQAVEVMCFVPKRCN
                     DMMTLGRLRGFEGKLTAQGKLLGQDTFWVTEPEAGGLLSSRGRERRVFLFEQIIIFSE
                     ALGGGVRGGTQPGYVYKNSIKVSCLGLEGNLQGDPCRFALTSRGPEGGIQRYVLQAAD
                     PAISQAWIKHVAQILESQRDFLNALQSPIEYQRRESQTNSLGRPRGPGVGSPGRIQLG
                     DQAQGSTHTPINGSLPSLLLSPKGEVARALLPLDKQALGDIPQAPHDSPPVSPTPKTP
                     PCQARLAKLDEDEL"
     exon            558..772
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            773..868
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            869..945
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1012
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1116
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1117..1186
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1276
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1369
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1420
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1498
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1499..1699
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..1880
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1881..2092
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2093..2554
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2532..2537
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /note="hexamer: AATAAA"
     polyA_site      2554
                     /gene="ARHGEF25"
                     /gene_synonym="GEFT; p63RhoGEF"
                     /note="major polyA site"
ORIGIN      
        1 attcagctct ccgctccgct gggacccgca cagcgccagt ggctcggggg tcggccctcg
       61 cctcctcccc ggcccgggcc tagagccacc cctaaccaga ggccggagac agctggagcg
      121 gctgccgcat cccggcccag cctcccctac catccctccc ccttccactg gacatctgga
      181 ccctaggccc ccgcaccgac agggttccgg aaaccctccc cgcccagccc ggcggccggg
      241 ccccgcgcct ctctctctct agagccccca gccctcctca agactagact tccgcctacc
      301 cctggacacc tcctcccggt cccctccctc cccccctccc acagcctgga ccggggtagg
      361 agggaggggg ggtgtgcgcc cggcgccgtc cccgccccgc cccccgtgat tccccctgca
      421 tggccggccc gggtgggggg cgcggggggg cccgggcgcc atgcgggggg ggcacaaagg
      481 gggtcgctgt gcctgtcccc gtgtgatccg aaaagtgctg gcaaaatgcg gctgctgctt
      541 cgcccggggg ggacgtgaat cctattccat tgcgggcagt gaggggagta tatcggcttc
      601 tgctgcctcc ggtctggctg ccccctctgg ccccagctct ggcctcagct ctggcccctg
      661 ttccccaggc cccccagggc ccgtcagtgg cctgaggaga tggttggatc attccaaaca
      721 ttgtctcagt gtggaaactg aggcagacag tggtcaggca ggaccatatg agaactggat
      781 gttggagcca gctctagcca caggagagga gctgccggaa ctgaccttgc tgaccacact
      841 gttggagggc cctggagata agacgcagcc acctgaagag gagactttgt cccaagcccc
      901 tgagagtgag gaggaacaga agaagaaggc tctggaaagg agtatgtatg tcctgagtga
      961 actggtagaa acagagaaaa tgtacgtgga cgacttgggg cagattgtgg agggttatat
     1021 ggccaccatg gctgctcagg gggtccccga gagtcttcga ggccgtgaca ggattgtgtt
     1081 tgggaatatc cagcaaatct atgagtggca ccgagactat ttcttgcaag agctacaacg
     1141 gtgtctgaaa gatcctgatt ggctggctca gctattcatc aaacacgagc gccggctgca
     1201 tatgtatgtg gtgtactgtc agaataagcc caagtcagag catgtggtgt cagagtttgg
     1261 ggacagctac tttgaggagc tccggcagca gctggggcac cgcctgcagc tgaacgacct
     1321 cctcatcaaa cctgtgcagc ggatcatgaa ataccagctg ctgctcaagg attttctcaa
     1381 gtattacaat agagctggga tggatactgc agacctagag caagctgtgg aggtcatgtg
     1441 ctttgtgccc aagcgctgca acgatatgat gacgctgggg agattgcggg gatttgaggg
     1501 caaactgact gctcagggga agctcttggg ccaggacact ttctgggtca ccgagcctga
     1561 ggctggaggg ctgctgtctt cccgaggtcg agagaggcgc gtcttcctct ttgagcaaat
     1621 catcatcttc agtgaagccc tgggaggagg agtgagaggt ggaacacagc ctggatatgt
     1681 atacaagaac agcattaagg tgagctgcct gggactggag gggaacctcc aaggtgaccc
     1741 ttgccgcttt gcactgacct ccagagggcc agagggtggg atccagcgct atgtcctgca
     1801 ggctgcagac cctgctatca gtcaggcctg gatcaagcat gtggctcaga tcttggagag
     1861 ccaacgggac ttcctcaacg cattgcagtc acccattgag taccagagac gggagagcca
     1921 gaccaacagc ctggggcggc caagagggcc tggagtgggg agccctggaa gaattcagct
     1981 tggagatcag gcccagggca gcacacacac acccatcaat ggctctctcc cctctctgct
     2041 gctgtcaccc aaaggggagg tggccagagc cctcttgcca ctggataaac aggcccttgg
     2101 tgacatcccc caggctcccc atgactctcc tccagtctct ccaactccaa aaacccctcc
     2161 ctgccaagcc agacttgcca agctggatga agatgagctg taactggtga aaaccatggg
     2221 ggtggtgctg actcagccgc ctattcccca aggagcttca gggcagtcct tctggcactg
     2281 ctccagaatt cctccttctt ggtgtgtctg gagggtgggc aaggctggga gggatatcaa
     2341 cttggaggag aacacctaga cccaaggact tttttctgcc caaggaacac agtttccttc
     2401 agctcccatc cctatgcatg catcatggtc cccccaaaag gaggatatgt gggtgggtgg
     2461 gagggctggg gcaggggcca gatagaaatt attggttttg ttttttaatt ttgtttttcc
     2521 tgttttctga gaataaaggt tttgttatat cacc
//
