LOCUS       NM_001350110            4260 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens Rho/Rac guanine nucleotide exchange factor 2
            (ARHGEF2), transcript variant 4, mRNA.
ACCESSION   NM_001350110 XM_005245592
VERSION     NM_001350110.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Luan,Y., Deng,Z., Zhu,Y., Dai,L., Yang,Y. and Xia,Z.
  TITLE     Decoupling actin assembly from microtubule disassembly by
            TBC1D3C-mediated direct GEF-H1 activation
  JOURNAL   Life Sci Alliance 8 (1), e202402585 (2024)
   PUBMED   39467635
  REMARK    GeneRIF: Decoupling actin assembly from microtubule disassembly by
            TBC1D3C-mediated direct GEF-H1 activation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Smart,K., Kramer,A.H., Smart,S., Hodgson,L. and Sharp,D.J.
  TITLE     Fidgetin-like 2 depletion enhances cell migration by regulating
            GEF-H1, RhoA, and FAK
  JOURNAL   Biophys J 122 (18), 3600-3610 (2023)
   PUBMED   36523161
  REMARK    GeneRIF: Fidgetin-like 2 depletion enhances cell migration by
            regulating GEF-H1, RhoA, and FAK.
REFERENCE   3  (bases 1 to 4260)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   4  (bases 1 to 4260)
  AUTHORS   Hu,Q., Lai,J., Chen,H., Cai,Y., Yue,Z., Lin,H. and Sun,L.
  TITLE     Reducing GEF-H1 Expression Inhibits Renal Cyst Formation,
            Inflammation, and Fibrosis via RhoA Signaling in Nephronophthisis
  JOURNAL   Int J Mol Sci 24 (4), 3504 (2023)
   PUBMED   36834937
  REMARK    GeneRIF: Reducing GEF-H1 Expression Inhibits Renal Cyst Formation,
            Inflammation, and Fibrosis via RhoA Signaling in Nephronophthisis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4260)
  AUTHORS   Chen,X., Shao,Y., Wei,W., Zhu,S., Li,Y., Chen,Y., Li,H., Tian,H.,
            Sun,G., Niu,Y. and Shang,Z.
  TITLE     Androgen deprivation restores ARHGEF2 to promote neuroendocrine
            differentiation of prostate cancer
  JOURNAL   Cell Death Dis 13 (11), 927 (2022)
   PUBMED   36335093
  REMARK    GeneRIF: Androgen deprivation restores ARHGEF2 to promote
            neuroendocrine differentiation of prostate cancer.
            Erratum:[Cell Death Dis. 2023 Oct 2;14(10):647. doi:
            10.1038/s41419-023-06051-0.. PMID: 37783705]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4260)
  AUTHORS   Krendel,M., Zenke,F.T. and Bokoch,G.M.
  TITLE     Nucleotide exchange factor GEF-H1 mediates cross-talk between
            microtubules and the actin cytoskeleton
  JOURNAL   Nat Cell Biol 4 (4), 294-301 (2002)
   PUBMED   11912491
  REMARK    GeneRIF: GEF-H1 is regulated by an interaction with microtubules.
REFERENCE   7  (bases 1 to 4260)
  AUTHORS   Harrington,A.W., Kim,J.Y. and Yoon,S.O.
  TITLE     Activation of Rac GTPase by p75 is necessary for c-jun N-terminal
            kinase-mediated apoptosis
  JOURNAL   J Neurosci 22 (1), 156-166 (2002)
   PUBMED   11756498
REFERENCE   8  (bases 1 to 4260)
  AUTHORS   Gao,Y., Xing,J., Streuli,M., Leto,T.L. and Zheng,Y.
  TITLE     Trp(56) of rac1 specifies interaction with a subset of guanine
            nucleotide exchange factors
  JOURNAL   J Biol Chem 276 (50), 47530-47541 (2001)
   PUBMED   11595749
  REMARK    Erratum:[J Biol Chem. 2005 Jan 14;280(2):1704]
REFERENCE   9  (bases 1 to 4260)
  AUTHORS   Ren,Y., Li,R., Zheng,Y. and Busch,H.
  TITLE     Cloning and characterization of GEF-H1, a microtubule-associated
            guanine nucleotide exchange factor for Rac and Rho GTPases
  JOURNAL   J Biol Chem 273 (52), 34954-34960 (1998)
   PUBMED   9857026
REFERENCE   10 (bases 1 to 4260)
  AUTHORS   Reddy,A.B., Chatterjee,A., Rothblum,L.I., Black,A. and Busch,H.
  TITLE     Isolation and characterization of complementary DNA to
            proliferating cell nucleolar antigen P40
  JOURNAL   Cancer Res 49 (7), 1763-1767 (1989)
   PUBMED   2466560
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA344419.1, BM476546.1, AL527706.3, AL512715.1 and BM552445.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001350110.1.
            
            Summary: Rho GTPases play a fundamental role in numerous cellular
            processes that are initiated by extracellular stimuli that work
            through G protein coupled receptors. The encoded protein may form
            complex with G proteins and stimulate rho-dependent signals.
            Alternatively spliced transcript variants encoding different
            isoforms have been identified.[provided by RefSeq, Jun 2009].
            
            Transcript Variant: This variant (4) contains an alternate 5' exon,
            resulting in a distinct 5' UTR, use of a downstream start codon,
            and uses two alternate in-frame splice sites in the 5' and 3'
            coding regions, compared to variant 1. The resulting isoform (4)
            has a shorter N-terminus, and contains an internal segment compared
            to isoform 1. Variants 4 and 5 encode the same protein (isoform 4).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.1591994.1,
                                           SRR1163657.532285.1 [ECO:0000332]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-244               DA344419.1         135-378
            245-810             BM476546.1         168-733
            811-967             AL527706.3         767-923
            968-2381            AL512715.1         815-2228
            2382-3157           BM552445.1         1-776
            3158-4260           AL512715.1         2975-4077
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q22"
     gene            1..4260
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /note="Rho/Rac guanine nucleotide exchange factor 2"
                     /db_xref="GeneID:9181"
                     /db_xref="HGNC:HGNC:682"
                     /db_xref="MIM:607560"
     exon            1..244
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            245..389
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     CDS             263..3172
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /note="isoform 4 is encoded by transcript variant 4;
                     proliferating cell nucleolar antigen p40; rho guanine
                     nucleotide exchange factor 2; microtubule-regulated
                     Rho-GEF; guanine nucleotide exchange factor H1; Rho/Rac
                     guanine nucleotide exchange factor (GEF) 2"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 2 isoform
                     4"
                     /protein_id="NP_001337039.1"
                     /db_xref="GeneID:9181"
                     /db_xref="HGNC:HGNC:682"
                     /db_xref="MIM:607560"
                     /translation="MKEAKDARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTC
                     NVTIHNRCKDTLANCTKVKQKQQKAALLKNNTALQSVSLRSKTTIRERPSSAIYPSDS
                     FRQSLLGSRRGRSSLSLAKSVSTTNIAGHFNDESPLGLRRILSQSTDSLNMRNRTLSV
                     ESLIDEAEVIYSELMSDFEMDEKDFAADSWSLAVDSSFLQQHKKEVMKQQDVIYELIQ
                     TELHHVRTLKIMTRLFRTGMLEELHLEPGVVQGLFPCVDELSDIHTRFLSQLLERRRQ
                     ALCPGSTRNFVIHRLGDLLISQFSGPSAEQMCKTYSEFCSRHSKALKLYKELYARDKR
                     FQQFIRKVTRPAVLKRHGVQECILLVTQRITKYPLLISRILQHSHGIEEERQDLTTAL
                     GLVKELLSNVDEGIYQLEKGARLQEIYNRMDPRAQTPVPGKGPFGREELLRRKLIHDG
                     CLLWKTATGRFKDVLVLLMTDVLVFLQEKDQKYIFPTLDKPSVVSLQNLIVRDIANQE
                     KGMFLISAAPPEMYEVHTASRDDRSTWIRVIQQSVRTCPSREDFPLIETEDEAYLRRI
                     KMELQQKDRALVELLREKVGLFAEMTHFQAEEDGGSGMALPTLPRGLFRSESLESPRG
                     ERLLQDAIREVEGLKDLLVGPGVELLLTPREPALPLEPDSGGNTSPGVTANGEARTFN
                     GSIELCRADSDSSQRDRNGNQLRSPQEEALQRLVNLYGLLHGLQAAVAQQDTLMEARF
                     PEGPERREKLCRANSRDGEAGRAGAAPVAPEKQATELALLQRQHALLQEELRRCRRLG
                     EERATEAGSLEARLRESEQARALLEREAEEARRQLAALGQTEPLPAEAPWARRPVDPR
                     RRSLPAGDALYLSFNPPQPSRGTDRLDLPVTTRSVHRNFEDRERQELGSPEERLQDSS
                     DPDTGSEEEGSSRLSPPHSPRGETLAETWTRDFTRMQDIPEETESRDGEAVASES"
     exon            390..457
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            458..521
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            522..651
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            652..761
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            762..905
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            906..1156
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1282
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1400
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1649
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1726
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..1896
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            1897..1964
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            1965..2165
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            2166..2285
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            2286..2353
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            2354..2389
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            2390..2440
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            2441..2884
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            2885..3098
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     exon            3099..4260
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4241..4246
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /note="hexamer: ATTAAA"
     polyA_site      4260
                     /gene="ARHGEF2"
                     /gene_synonym="GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM;
                     P40"
                     /note="major polyA site"
ORIGIN      
        1 ctctcctctc tgttcttcga cctctgggag tgaatcctac ccttcccgtg tactgaagat
       61 ccagcgttta gttctccttt gatttcctct ccctctggcc acccctgccc ccaatcgtaa
      121 aaggggtcag tctgctcagg cctgctttga tgggaccccg aaggccagga aggaactggt
      181 cctgggtttt ctaagagctg ggggcggatg gaattctcct gcctgcgggt ctttaaagac
      241 tgagacccgg gaaaaggaga agatgaagga agccaaggat gcccgctata ccaatgggca
      301 cctcttcacc accatttcag tttcaggcat gaccatgtgc tatgcctgta acaagagcat
      361 cacagccaag gaagccctca tctgcccaac ctgcaatgtg actatccaca accgctgtaa
      421 agacaccctc gccaactgta ccaaggtcaa gcagaagcaa cagaaagcgg ccctgctgaa
      481 gaacaacacc gccttgcagt ccgtttctct tcgaagtaag acaaccatcc gggagcggcc
      541 aagctcggcc atctacccct ccgacagctt ccggcagtcc ctcctgggct cccgccgtgg
      601 ccgctcctcc ttgtctttag ccaagagtgt ttctaccacc aacattgctg gacatttcaa
      661 tgatgagtct cccctggggc tgcgccggat cctctcacag tccacagact ccctcaacat
      721 gcggaaccga accctatccg tggaatccct cattgacgaa gcagaggtaa tctacagtga
      781 gctgatgagt gactttgaga tggatgagaa ggactttgca gctgactctt ggagtcttgc
      841 tgtggacagc agcttcctgc agcagcataa aaaggaggtg atgaagcagc aagatgtcat
      901 ctatgagcta atccagacag agctgcacca tgtgaggaca ctgaagatca tgacccgcct
      961 cttccgcacg gggatgctgg aagagctaca cttggagcca ggagtggtcc agggcctgtt
     1021 cccctgcgtg gacgagctca gtgacatcca tacacgcttc ctcagccagc tattagaacg
     1081 ccgacgccag gccctgtgcc ctggcagcac ccggaacttt gtcatccatc gcttgggtga
     1141 tctgctcatc agccagttct caggtcctag tgcggagcag atgtgtaaga cctactcgga
     1201 gttctgcagc cgccacagca aggccttaaa gctctataag gagctgtacg cccgagacaa
     1261 acgcttccag caattcatcc ggaaagtgac ccgccccgcc gtgctcaagc ggcacggggt
     1321 acaggagtgc atcctgctgg tgactcagcg catcaccaag tacccgttac tcatcagccg
     1381 catcctgcag cattcccacg ggatcgagga ggagcgccag gacctgacca cagcactggg
     1441 gctagtgaag gagctgctgt ccaatgtgga cgagggtatt tatcagctgg agaaaggggc
     1501 ccgtctgcag gagatctaca accgcatgga ccctcgggcc caaaccccag tgcctggcaa
     1561 gggccccttt ggccgagagg aacttctgag gcgcaaactc atccacgatg gctgcctgct
     1621 ctggaagaca gcgacggggc gcttcaaaga tgtgctagtg ctgctgatga cagatgtact
     1681 ggtgtttctc caggaaaagg accagaagta catctttcct accctggaca agccttcagt
     1741 ggtatcgctg cagaatctaa tcgtacgaga cattgccaac caggagaaag ggatgtttct
     1801 gatcagcgca gccccacctg agatgtacga ggtgcacaca gcatcccggg atgaccggag
     1861 cacctggatc cgggtcattc agcagagcgt gcgcacatgc ccatccaggg aggacttccc
     1921 cctgattgag acagaggatg aggcttacct gcggcgaatt aagatggagt tgcagcagaa
     1981 ggaccgggca ctggtggagc tgctgcgaga gaaggtcggg ctgtttgctg agatgaccca
     2041 tttccaggcc gaagaggatg gtggcagtgg gatggccctg cccaccctgc ccaggggcct
     2101 tttccgctct gagtcccttg agtcccctcg tggcgagcgg ctgctgcagg atgccatccg
     2161 tgaggtggag ggtctgaaag acctgctggt ggggccagga gtggaactgc tcttgacacc
     2221 ccgagagcca gccctgccct tggaaccaga cagcggtggt aacacgagtc ctggggtcac
     2281 tgccaatggt gaggccagaa ccttcaatgg ctccattgaa ctctgcagag ctgactcaga
     2341 ctctagccag agggatcgaa atggaaatca gctgagatca ccgcaagagg aggcgttaca
     2401 gcgattggtc aatctctatg gacttctaca tggcctacag gcagctgtgg cccagcagga
     2461 cactctgatg gaagcccggt tccctgaggg ccctgagcgg cgggagaagc tgtgccgagc
     2521 caactctcgg gatggggagg ctggcagggc tggggctgcc cctgtggccc ctgaaaagca
     2581 ggccacggaa ctggcattac tgcagcggca acatgcgctg ctgcaggagg agctacggcg
     2641 ctgccggcgg ctaggtgaag aacgggcaac cgaagctggc agcctggagg cccggctccg
     2701 ggagagtgag caggcccggg cactgctgga gcgtgaggcc gaagaggctc gaaggcagct
     2761 ggccgccctg ggccagaccg agccactccc agctgaggcc ccctgggccc gcagacctgt
     2821 ggatcctcgg cggcgcagcc tccccgcagg cgatgccctg tacttgagtt tcaacccccc
     2881 acagcccagc cgaggcactg accgcctgga tctacctgtc actactcgct ctgtccatcg
     2941 aaactttgag gaccgagaga ggcaggaact ggggagcccc gaagagcggc tgcaagacag
     3001 cagtgaccct gacactggca gcgaggagga aggtagcagc cgtctgtctc cgccccacag
     3061 tccacgaggt gagaccctgg cggagacatg gaccagagac tttaccagaa tgcaggacat
     3121 cccggaggag acggagagcc gcgacgggga ggctgtagcc tccgagagct aagggggccc
     3181 ctcccccctg ccccgtgccc cactgaagaa cattactgag ggggctaacc ttggggactc
     3241 caatttgcca atgatgaggg aacatttgaa agaactgcaa attgtccttg ccagctcttg
     3301 ggatccttgg atacctgggg ccatttaaga agctagggga attaggccac aacaccccct
     3361 gggacatccg aaagctacac cacagatgcc agtggttcat gccttcttcc cgcaacttta
     3421 ggaaaattta tttatttatt gtttattagt tatgggggga gaggggagat ttaaaggacc
     3481 agggacatgg gaaccaagcc atagggatca gagggccttg tccttgaaca ctactggggt
     3541 atattcaggc tcatccacgc agctgctggg ttcttgccct aacggccctc ccctgcaaca
     3601 tccgtcttgg aggagaggct gcagccacag caccctactg ccctttaaat aaaggagggc
     3661 tgtgggcagg gccatgtccc tttctcctct cccctcaacc tcttactgct gttctccctt
     3721 tctccgtcct tcatggaagc cctgggagat aacctggctt cctggagttg atggaataaa
     3781 ggttggggtg gccataatgg tttgttgggg gtgagggaaa aaacccacag ggaccagaat
     3841 gttttgttgt tcttttgttt tcttttttgt accaaagtca actgcacgtg ttttatattt
     3901 ttaagagatc gtaggcaatt agagatcgaa gcctcctatc tccacatctc tgaagaagtt
     3961 gaggggtggg ggagagaatg acttctgcct tcatctgcag taacgggggg acctatactg
     4021 acctcttccc cagccattta gaaacaagtt ctagggtggg ttggaaaatc tccaagagcc
     4081 ctgacctcat cttccacctc agcaaccatg acctgaaacc tcagcgtgaa tttgggggat
     4141 ttttcagtgg aacccttgcc cccaaatgtc gaccagcccc caaatgtcga agaattttct
     4201 tcttgccaat tttgttgttt aaaaaaaaaa ttcagggaaa attaaaaacc tggaactcca
//
