LOCUS       NM_001367823            6681 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens Rho/Rac guanine nucleotide exchange factor 18
            (ARHGEF18), transcript variant 3, mRNA.
ACCESSION   NM_001367823 XM_006722705
VERSION     NM_001367823.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Li,D., Sun,Y., Kong,X., Luan,C., Yu,Y., Chen,F. and Chen,P.
  TITLE     Association between a Single Nucleotide Polymorphism in the 3'-UTR
            of ARHGEF18 and the Risk of Nonidiopathic Pulmonary Arterial
            Hypertension in Chinese Population
  JOURNAL   Dis Markers 2018, 2461845 (2018)
   PUBMED   30405854
  REMARK    GeneRIF: ARHGEF18 rs3745357 variant is associated with
            nonidiopathic pulmonary arterial hypertension susceptibility in
            Chinese population.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6681)
  AUTHORS   Turton,K.B., Wilkerson,E.M., Hebert,A.S., Fogerty,F.J.,
            Schira,H.M., Botros,F.E., Coon,J.J. and Mosher,D.F.
  TITLE     Expression of novel 'LOCGEF' isoforms of ARHGEF18 in eosinophils
  JOURNAL   J Leukoc Biol 104 (1), 135-145 (2018)
   PUBMED   29601110
  REMARK    GeneRIF: We suggest that a portion of a 1361-residue isoform of
            ARHGEF18, which is unlike any other protein domain, has unique
            functions in control of polarity in activated eosinophils and other
            leukocytes
REFERENCE   4  (bases 1 to 6681)
  AUTHORS   Arno,G., Carss,K.J., Hull,S., Zihni,C., Robson,A.G., Fiorentino,A.,
            Hardcastle,A.J., Holder,G.E., Cheetham,M.E., Plagnol,V.,
            Moore,A.T., Raymond,F.L., Matter,K., Balda,M.S. and Webster,A.R.
  CONSRTM   UK Inherited Retinal Disease Consortium; NIHR Bioresource - Rare
            Diseases Consortium
  TITLE     Biallelic Mutation of ARHGEF18, Involved in the Determination of
            Epithelial Apicobasal Polarity, Causes Adult-Onset Retinal
            Degeneration
  JOURNAL   Am J Hum Genet 100 (2), 334-342 (2017)
   PUBMED   28132693
  REMARK    GeneRIF: mutation of ARHGEF18 is a likely cause of inherited
            retinal dystrophy
REFERENCE   5  (bases 1 to 6681)
  AUTHORS   Loie,E., Charrier,L.E., Sollier,K., Masson,J.Y. and Laprise,P.
  TITLE     CRB3A Controls the Morphology and Cohesion of Cancer Cells through
            Ehm2/p114RhoGEF-Dependent Signaling
  JOURNAL   Mol Cell Biol 35 (19), 3423-3435 (2015)
   PUBMED   26217016
  REMARK    GeneRIF: CRB3A recruits p114RhoGEF and its activator Ehm2 to the
            cell periphery using both functional motifs of its cytoplasmic tail
            and increases RhoA activation levels.
REFERENCE   6  (bases 1 to 6681)
  AUTHORS   Rabizadeh,S. and Bredesen,D.E.
  TITLE     Ten years on: mediation of cell death by the common neurotrophin
            receptor p75(NTR)
  JOURNAL   Cytokine Growth Factor Rev 14 (3-4), 225-239 (2003)
   PUBMED   12787561
  REMARK    Review article
REFERENCE   7  (bases 1 to 6681)
  AUTHORS   Salehi,A.H., Xanthoudakis,S. and Barker,P.A.
  TITLE     NRAGE, a p75 neurotrophin receptor-interacting protein, induces
            caspase activation and cell death through a JNK-dependent
            mitochondrial pathway
  JOURNAL   J Biol Chem 277 (50), 48043-48050 (2002)
   PUBMED   12376548
REFERENCE   8  (bases 1 to 6681)
  AUTHORS   Harrington,A.W., Kim,J.Y. and Yoon,S.O.
  TITLE     Activation of Rac GTPase by p75 is necessary for c-jun N-terminal
            kinase-mediated apoptosis
  JOURNAL   J Neurosci 22 (1), 156-166 (2002)
   PUBMED   11756498
REFERENCE   9  (bases 1 to 6681)
  AUTHORS   Acierno,J.S. Jr., Kennedy,J.C., Falardeau,J.L., Leyne,M.,
            Bromley,M.C., Colman,M.W., Sun,M., Bove,C., Ashworth,L.K.,
            Chadwick,L.H., Schiripo,T., Ma,S., Goldin,E., Schiffmann,R. and
            Slaugenhaupt,S.A.
  TITLE     A physical and transcript map of the MCOLN1 gene region on human
            chromosome 19p13.3-p13.2
  JOURNAL   Genomics 73 (2), 203-210 (2001)
   PUBMED   11318610
REFERENCE   10 (bases 1 to 6681)
  AUTHORS   Blomquist,A., Schworer,G., Schablowski,H., Psoma,A., Lehnen,M.,
            Jakobs,K.H. and Rumenapp,U.
  TITLE     Identification and characterization of a novel Rho-specific guanine
            nucleotide exchange factor
  JOURNAL   Biochem J 352 Pt 2 (Pt 2), 319-325 (2000)
   PUBMED   11085924
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC119396.3 and AC008878.10.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Dec 19, 2018 this sequence version replaced XM_006722705.3.
            
            Summary: Rho GTPases are GTP binding proteins that regulate a wide
            spectrum of cellular functions. These cellular processes include
            cytoskeletal rearrangements, gene transcription, cell growth and
            motility. Activation of Rho GTPases is under the direct control of
            guanine nucleotide exchange factors (GEFs). The protein encoded by
            this gene is a guanine nucleotide exchange factor and belongs to
            the Rho GTPase GEF family. Family members share a common feature, a
            Dbl (DH) homology domain followed by a pleckstrin (PH) homology
            domain. Alternatively spliced transcript variants encoding
            different isoforms have been identified. [provided by RefSeq, Nov
            2018].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1965299,
                              SAMEA1966682 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000668164.2/ ENSP00000499655.2
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-305               AC119396.3         15069-15373
            306-430             AC119396.3         28913-29037
            431-690             AC119396.3         38944-39203
            691-841             AC119396.3         41852-42002
            842-956             AC119396.3         42775-42889
            957-1014            AC119396.3         44526-44583
            1015-1059           AC119396.3         45254-45298
            1060-1137           AC119396.3         47049-47126
            1138-1240           AC119396.3         48924-49026
            1241-1382           AC119396.3         49194-49335
            1383-1521           AC008878.10        54268-54406
            1522-1634           AC008878.10        55577-55689
            1635-1775           AC008878.10        55836-55976
            1776-2026           AC008878.10        58128-58378
            2027-2152           AC008878.10        60967-61092
            2153-2270           AC008878.10        65073-65190
            2271-2519           AC008878.10        67391-67639
            2520-2596           AC008878.10        70251-70327
            2597-2775           AC008878.10        72436-72614
            2776-2867           AC008878.10        73827-73918
            2868-3050           AC008878.10        76076-76258
            3051-3188           AC008878.10        77742-77879
            3189-3319           AC008878.10        78484-78614
            3320-3376           AC008878.10        80842-80898
            3377-3424           AC008878.10        80995-81042
            3425-3895           AC008878.10        81138-81608
            3896-4202           AC008878.10        82749-83055
            4203-4328           AC008878.10        83828-83953
            4329-6681           AC008878.10        84050-86402
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.2"
     gene            1..6681
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /note="Rho/Rac guanine nucleotide exchange factor 18"
                     /db_xref="GeneID:23370"
                     /db_xref="HGNC:HGNC:17090"
                     /db_xref="MIM:616432"
     exon            1..305
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            306..430
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     CDS             416..4501
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /note="isoform c is encoded by transcript variant 3;
                     Rho-specific guanine nucleotide exchange factor p114;
                     septin-associated RhoGEF; 114 kDa Rho-specific guanine
                     nucleotide exchange factor; Rho/Rac guanine nucleotide
                     exchange factor (GEF) 18"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 18
                     isoform c"
                     /protein_id="NP_001354752.1"
                     /db_xref="CCDS:CCDS92502.1"
                     /db_xref="GeneID:23370"
                     /db_xref="HGNC:HGNC:17090"
                     /db_xref="MIM:616432"
                     /translation="MGDDQEDDFPRRLSESMEDLSLDLGALQGSEYLQDLGLGAPSHS
                     QPGETPDSRPTGEEPGRDSLFSSLAGSQDLSRRRSWERSRSCSESWRRLSLDASAVDE
                     EPCLPRTLASLALNLPGGGLKTWTQGCLSGGGTPAESPGKECDSPKKRGRSRSVPVSF
                     YEIRSPEISPGLEVPTPPVQGLEPPVLECMEKDHVEPDHVLIVQQVLQELRQYHGARQ
                     RACMSASPGGAHSNLTWFEFLSESEDGAGKNEKSDKSTSVKRRLSCLRSRVTRQKEKG
                     KSPAHLKDKGQDARERRECVNGHQLLQGTFSGPSSCPLCGKPFLSSASLKEHPRGTLL
                     SDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQLGPITGEMDEADSAFLKFKQTADDS
                     LSLTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQD
                     VLYELMQTEVHHVRTLKIMLKVYSRALQEELQFSSKAIGRLFPCADDLLETHSHFLAR
                     LKERRQESLEEGSDRNYVIQKIGDLLVQQFSGENGERMKEKYGVFCSGHNEAVSHYKL
                     LLQQNKKFQNLIKKIGNFSIVRRLGVQECILLVTQRITKYPVLVERIIQNTEAGTEDY
                     EDLTQALNLIKDIISQVDAKVSECEKGQRLREIAGKMDLKSSSKLKNGLTFRKEDMLQ
                     RQLHLEGMLCWKTTSGRLKDILAILLTDVLLLLQEKDQKYVFASVDSKPPVISLQKLI
                     VREVANEEKAMFLISASLQGPEMYEIYTSSKEDRNAWMAHIQRAVESCPDEEEGPFSL
                     PEEERKVVEARATRLRDFQERLSMKDQLIAQSLLEKQQIYLEMAEMGGLEDLPQPRGL
                     FRGGDPSETLQGELILKSAMSEIEGIQSLICRQLGSANGQAEDGGSSTGPPRRAETFA
                     GYDCTNSPTKNGSFKKKVSSTDPRPRDWRGPPNSPDLKLSDSDIPGSSEESPQVVEAP
                     GTESDPRLPTVLESELVQRIQTLSQLLLNLQAVIAHQDSYVETQRAAIQEREKQFRLQ
                     STRGNLLLEQERQRNFEKQREERAALEKLQSQLRHEQQRWERERQWQHQELERAGARL
                     QEREGEARQLRERLEQERAELERQRQAYQHDLERLREAQRAVERERERLELLRRLKKQ
                     NTAPGALPPDTLAEAQPPSHPPSFNGEGLEGPRVSMLPSGVGPEYAERPEVARRDSAP
                     TENRLAKSDVPIQLLSATNQFQRQAAVQQQIPTKLAASTKGGKDKGGKSRGSQRWESS
                     ASFDLKQQLLLNKLMGKDESTSRNRRSLSPILPGRHSPAPPPDPGFPAPSPPPADSPS
                     EGFSLKAGGTALLPGPPAPSPLPATPLSAKEDASKEDVIFF"
     exon            431..690
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            691..841
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            842..956
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1014
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1059
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1137
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1240
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1382
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1521
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1634
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1775
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..2026
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2027..2152
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2270
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2271..2519
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2520..2596
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2597..2775
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2776..2867
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2868..3050
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3051..3188
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3189..3319
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3320..3376
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3377..3424
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3425..3895
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3896..4202
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4203..4328
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            4329..6681
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6662..6667
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /note="hexamer: AATAAA"
     polyA_site      6681
                     /gene="ARHGEF18"
                     /gene_synonym="P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF"
                     /note="major polyA site"
ORIGIN      
        1 aagacacctg catgcgcagg gctgaggggt gggagcaggg ctctctgcct ccagaacccc
       61 catcctctgg gctcttttta ggcccctaga ttggcctgca gctgggagca gagtggcaag
      121 catgcaggca ggcagttgag aaagaggaag tcgaggtgct attgggtcat tctgtggtta
      181 tcccaggctc tacccagcca gcggttccct tgggccagct cgaaacgctg gccgtaggtc
      241 atgggagctg caggagctgt tagccaggct aggtgcctgc aggttgaaga actgagctct
      301 atctggctct gagcccaagt catgtgtcca gccttttgac tctggagctg tggcttcagc
      361 caccaagagc agcagtggat cctggaaacc tgagaaccca gacttcttct ctgccatggg
      421 ggatgatcag gaggatgatt ttcccaggcg gctcagcgag agtatggagg acctcagcct
      481 ggatttgggg gcccttcagg gcagcgagta tctgcaggac ctgggccttg gggccccttc
      541 ccacagccag cctggggaga ccccagacag ccgccccacc ggtgaagaac caggaagaga
      601 ttctcttttc tccagcttgg cagggtccca agacctgtca aggcggcgca gctgggaaag
      661 gtcgcggagc tgctcagaga gctggcggag gctcagcctc gatgcctcag ctgtggatga
      721 ggaaccctgt ctcccccgaa cactggccag ccttgctttg aacctgccag gaggagggct
      781 gaagacctgg actcaagggt gtctctctgg gggcgggacc cccgcagaga gcccaggcaa
      841 ggaatgtgac agccccaaga aaagagggag gtcaaggtcc gttcctgtgt ccttctatga
      901 gatccgctct ccggaaatct ctccgggttt ggaagtgccc actccacctg ttcaaggcct
      961 ggaacctcca gtgctggagt gcatggaaaa agaccatgtg gaaccagatc acgtgctgat
     1021 tgtccagcag gtgcttcaag aacttcgaca gtaccatggg gcccggcaga gggcttgcat
     1081 gtcagccagc cccggaggag cccactcgaa cctgacctgg tttgaattcc tgtcggagag
     1141 cgaggacggt gctggcaaga acgagaagag tgacaagagt accagtgtga agcgcaggct
     1201 gagctgcctc cgcagtcgag tgaccaggca gaaggagaag gggaagagcc cagcacatct
     1261 gaaggacaag ggccaggatg cacgagagag gcgggagtgt gtcaatgggc accagctgtt
     1321 gcaagggacc ttctccggcc cctccagctg ccccctgtgt ggcaaacctt tcttgagctc
     1381 agcctcgctc aaggagcacc cccggggcac cctcctgtcc gatggcagcc cggccctgtc
     1441 caggaatgtc ggtatgacgg tctctcagaa agggggtccc cagccaacac cgagcccggc
     1501 tggccctggg acgcaactcg gaccaatcac aggagagatg gatgaagccg attctgcgtt
     1561 tttaaaattt aagcagacag ctgatgactc tctgtccctt acatctccaa acaccgagtc
     1621 catttttgta gaagatccct acaccgcctc gctgaggagt gagattgagt cagacggcca
     1681 cgagtttgaa gctgagtcct ggagcctcgc cgtggatgca gcctacgcca agaagcaaaa
     1741 gagggaggtg gtgaaaagac aagatgtcct ttatgagctg atgcagacag aggtgcacca
     1801 cgtgcggacg ctcaagatca tgctgaaggt gtactccagg gccctgcagg aggagctgca
     1861 gttcagcagc aaggccattg gccgcctctt cccatgcgct gacgacctgc tggagacgca
     1921 cagccacttc ctcgctcggc tcaaggagcg ccgccaggag tccctggagg agggcagtga
     1981 ccggaattat gtcatccaga aaatcggcga cctcctggtt cagcagtttt caggtgaaaa
     2041 tggggagaga atgaaagaaa agtacggtgt gttttgtagt ggccacaatg aagctgttag
     2101 tcattacaag ttgctgcttc agcaaaacaa gaaatttcaa aacttgatca agaaaattgg
     2161 caacttctcc atcgtgcggc ggcttggcgt gcaggagtgc attctcctgg ttacacaacg
     2221 cataaccaaa tacccagtgc tggtggagcg catcatccag aacacggaag ctggcactga
     2281 ggactatgaa gacctgaccc aggccttgaa cctcatcaaa gatatcatct cacaagtgga
     2341 cgccaaggtc agtgagtgtg agaagggcca gcgcctcagg gagatcgcag ggaagatgga
     2401 cctgaagtct tccagcaaac tcaagaacgg gctcaccttc cgcaaggaag acatgcttca
     2461 gcggcagctc cacctggagg gcatgctatg ctggaagacc acatcagggc gcttgaaaga
     2521 tatcctggct atcctgctga ccgacgtact tttgctgcta caagaaaaag atcagaaata
     2581 cgtctttgct tctgtggact caaagccacc cgtcatctcg ttacaaaagc tcatcgtgag
     2641 ggaagtggcc aacgaggaga aagcgatgtt tctgatcagc gcctccttgc aagggccgga
     2701 gatgtatgaa atctacacga gctccaaaga ggacaggaac gcctggatgg cccacatcca
     2761 aagggctgtg gagagctgcc ctgacgagga ggaggggccc ttcagcctgc ccgaagagga
     2821 aaggaaggtg gtcgaggccc gcgccacgag actccgggac tttcaagagc ggttgagcat
     2881 gaaagaccag ctgatcgcac agagcctcct agagaaacag cagatctacc tggagatggc
     2941 cgagatgggc ggcctcgaag acctgcccca gccccgaggc ctattccgtg gaggggaccc
     3001 atccgagacc ctgcaggggg agctaattct caagtcggcc atgagcgaga tcgagggcat
     3061 ccagagcctg atctgcaggc agctgggcag cgccaacggc caggcggaag acggaggcag
     3121 ctccacaggc ccgcccagga gggctgagac cttcgcgggc tacgactgca caaacagccc
     3181 caccaagaat ggcagtttca agaagaaagt cagcagcact gaccccaggc cccgagactg
     3241 gcgaggcccc ccaaacagcc cggacttgaa gctcagtgac agtgacattc ctgggagctc
     3301 tgaggaatcg ccgcaggtgg tggaggcgcc aggcacggaa tccgatcccc gtctgcccac
     3361 cgtcctggag tcggagcttg tccagcggat ccagacactg tcccagctgc tcctgaacct
     3421 tcaggcggta atcgcccacc aggacagcta tgtggagacg cagcgggctg ccatccagga
     3481 gcgggagaag cagttccggc tgcagtcgac gcgtgggaac ctgctgctgg agcaggagcg
     3541 gcaacgcaac ttcgagaagc agcgggagga gcgcgcggcc ctggagaagc tgcagagcca
     3601 gctgcggcac gagcagcagc gctgggagcg cgagcgccag tggcagcacc aggagctgga
     3661 gcgtgcgggc gcgcggctgc aggagcgcga gggcgaggcg cggcagctac gcgagcggct
     3721 ggagcaggag cgggccgagc tggagcgcca gcgccaggcc taccagcacg acctggagcg
     3781 gctgcgcgag gcccagcgtg ccgtggagcg cgagcgggag cgcctggagc tgctgcgccg
     3841 cctcaagaag cagaacaccg cgccaggcgc gctgccgccc gacacactgg ccgaggccca
     3901 gcccccaagc caccctccca gcttcaacgg ggaagggctg gagggccctc gtgtgagcat
     3961 gctgccatcc ggcgtggggc cagagtacgc agagcgcccc gaggtggctc gccgggacag
     4021 cgcccccacc gagaaccggc tggccaagag cgatgtgccc atccagctgc tcagcgccac
     4081 caaccagttc cagaggcagg cggccgtgca gcagcagatc cccaccaagc tggcggcctc
     4141 caccaagggt ggcaaggaca agggcggcaa gagcaggggc tctcagcgct gggagagctc
     4201 agcgtccttc gacctgaagc agcagctgct gctcaacaag ctcatgggga aagatgagag
     4261 cacctcacgg aaccgccgct cgctgagccc tatcctgccc ggcagacaca gtcctgcgcc
     4321 cccaccagac cctggcttcc ccgccccgag cccaccgcca gctgacagcc cctccgaggg
     4381 cttctctctc aaggccgggg gcacagccct cctgcccggg cccccagctc cctcgccact
     4441 gccggccaca ccactcagcg ccaaggagga cgccagcaaa gaagacgtca tcttcttcta
     4501 aaagggccgt gactcaaggt gcaaggcccc tccctgccct gcccaccctt cctgctctct
     4561 ggggaccccc atggggtcac catgcccacc cagctgtccc ctcctcttcc ctagcaaacc
     4621 actgatgacc gcctggcagg ggccagcctg tcggtgctct gggccttgca gctgtttctg
     4681 tagggttagc ggtggtgccg gggtcacttt ctgaatctct tttttttttt ttcaaaaagg
     4741 aaagttttta atggaaagtt gagccagaac taaaccaggg agctgtctga aatcatagca
     4801 ccccatccgg gtggcgggga gatcaactcc gagctgtttt tccgaggcag tgaggaacgg
     4861 tgccggctct gcacggagct gaggacagga cagaccttgc tttgagaagg agctgccggc
     4921 cggggccacg ctccacagcc gccgcgcgac agtggagcca agggttaggg caccaggagg
     4981 ggccaggtgg cgtcggcagc atctgtcccc agaatcaggc agaatccact tcccaaacag
     5041 agccccacgc aggttcacca tgaacctcag ggtcagggaa tgagccaggc acgggggcat
     5101 gggcagagag ggccacgggg cagggcccac tgagggaaca tcagtggccc tccagtcagg
     5161 ttctgtgggt ttggaagccc atcgtgaaag gggctgacct ttgccccttt ttacttggca
     5221 ttggttttga aaccagctgt ttcccaaact ctgcttccca agggcaaccg ttgctgttca
     5281 cacgctcagc ctgtctgggg gagcgggcct ctagcttcag ccagggcggg tacacaccct
     5341 gggcacaggg tcctcagccc ccgggaaatg agctcccagg gctggcgtcc caccttccag
     5401 gtgggggctg gcacatcaca gactgtcgag agcgccatgt cccagggcat gcagaggatg
     5461 cacctagaga cgttgcagca agtggacaag tggccgctgt gcgggcccct cgcttgtagt
     5521 gagctgttgc agcttacggt ccgttccctg gaggggtgga ggaaggaggt gttgggcagc
     5581 atcaaaggtg ctgggacatc ccagggtggt gagatccatc cacgatccag ctccggtgga
     5641 gaaagggccc atgtcaagcc ttgttctgca ccccaagcat tggtggtagg actgggtcct
     5701 ggctgatcgt ccttgttccc agtggggtac atgtgagccc ctgccagggc caagtccttc
     5761 tcccgaaccc agggtcctgg gaactgcaga tcccgggggg attcagccct tctcccactg
     5821 tgctggcaga ggcactcctg tgacgctgaa tacagtgaac agggacattc ccgccactcg
     5881 gggacagatg ggcacaaggg aggggaaact ccatcaggaa gtgctcccct gggcagaggc
     5941 gcccactggg tgctgtgggc tcaggagggg gcggggcagg agctggtgcc aaccgggaac
     6001 cagagcccca cagccataca gcccattggt gacaaggtcc tgagaacaca gtggccaggt
     6061 gtccccaggc tcctggcccc tccgacgacc tcaactctgc ccagcccggt ccctggccat
     6121 cagcgacgct gtccgccccc cgtcagatcc catgtgtgcc atgtttatca tcagtgtttt
     6181 gtatttttgt actgagtatc ggagcacttt acagaagctg actgtacatt cctgttctgt
     6241 tgtgaagaga acattcccag accctggcac cctcctgagc cggcgtgtgc cggtccagcc
     6301 ctccgagatg ccacaattcc ttggatgggg gagaagttca aggaatttct gctcggccac
     6361 gcggtgggaa ccccgcgtcc ccgccatgtg gcagaggggt ctcagtcgtg ctaggcatcg
     6421 ggcggcagcg ccgacagccc ttccctcgcc agtgcccctc ggccactcct gggttggagc
     6481 ccgattttat ttgtaaagtt gacagtcgag caaatgttcc tattttcgtg ggatctgcac
     6541 acgtctttgt cagttgtggt catgatctta gtcacctgct aattattttt acaatgatta
     6601 caacatttcc tcactgcggg atatttctga cccgctttag aacttaagac ctgattctag
     6661 caataaacgt gtccgagatg a
//
