LOCUS       NM_173728               4196 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens Rho guanine nucleotide exchange factor 15 (ARHGEF15),
            transcript variant 1, mRNA.
ACCESSION   NM_173728 NM_014958
VERSION     NM_173728.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Sell,G.L., Schaffer,T.B. and Margolis,S.S.
  TITLE     Reducing expression of synapse-restricting protein Ephexin5
            ameliorates Alzheimer's-like impairment in mice
  JOURNAL   J Clin Invest 127 (5), 1646-1650 (2017)
   PUBMED   28346227
  REMARK    GeneRIF: pathological elevation of Ephexin5 expression critically
            drives Abeta-induced memory impairment
REFERENCE   3  (bases 1 to 4196)
  AUTHORS   Fukushima,H., Yasumoto,M., Ogasawara,S., Akiba,J., Kitasato,Y.,
            Nakayama,M., Naito,Y., Ishida,Y., Okabe,Y., Yasunaga,M.,
            Horiuchi,H., Sakamoto,E., Itadani,H., Mizuarai,S., Oie,S. and
            Yano,H.
  TITLE     ARHGEF15 overexpression worsens the prognosis in patients with
            pancreatic ductal adenocarcinoma through enhancing the motility and
            proliferative activity of the cancer cells
  JOURNAL   Mol Cancer 15 (1), 32 (2016)
   PUBMED   27145964
  REMARK    GeneRIF: upregulation of ARHGEF15 contributes to the development of
            aggressive PDAC by increasing the growth and motility of the
            pancreatic cancer cells, thereby worsening the prognosis of these
            patients.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4196)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   5  (bases 1 to 4196)
  AUTHORS   Kusuhara,S., Fukushima,Y., Fukuhara,S., Jakt,L.M., Okada,M.,
            Shimizu,Y., Hata,M., Nishida,K., Negi,A., Hirashima,M.,
            Mochizuki,N., Nishikawa,S. and Uemura,A.
  TITLE     Arhgef15 promotes retinal angiogenesis by mediating VEGF-induced
            Cdc42 activation and potentiating RhoJ inactivation in endothelial
            cells
  JOURNAL   PLoS One 7 (9), e45858 (2012)
   PUBMED   23029280
  REMARK    GeneRIF: Arhgef15 acts as an endothelial cell-specific GEF to
            mediate VEGF-induced Cdc42 activation and potentiate RhoJ
            inactivation, thereby promoting actin polymerization and cell
            motility.
REFERENCE   6  (bases 1 to 4196)
  AUTHORS   Dalva,M.B.
  TITLE     Ephecting excitatory synapse development
  JOURNAL   Cell 143 (3), 341-342 (2010)
   PUBMED   21029857
REFERENCE   7  (bases 1 to 4196)
  AUTHORS   Ogita,H., Kunimoto,S., Kamioka,Y., Sawa,H., Masuda,M. and
            Mochizuki,N.
  TITLE     EphA4-mediated Rho activation via Vsm-RhoGEF expressed specifically
            in vascular smooth muscle cells
  JOURNAL   Circ Res 93 (1), 23-31 (2003)
   PUBMED   12775584
  REMARK    GeneRIF: This paper presents functional studies determined in rat,
            mouse, and human tissues and cell lines.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB279954.1, AB020722.1,
            BC036749.1 and BQ877184.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_173728.3.
            
            Summary: Rho GTPases play a fundamental role in numerous cellular
            processes that are initiated by extracellular stimuli that work
            through G protein-coupled receptors. This gene encodes a protein
            that functions as a specific guanine nucleotide exchange factor for
            RhoA. It also interacts with ephrin A4 in vascular smooth muscle
            cells. Two alternatively spliced transcripts variants that encode
            the same protein have been found for this gene. [provided by
            RefSeq, Aug 2010].
            
            Transcript Variant: This variant (1) is the longer transcript. It
            encodes the same protein as variant 2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC036749.1, AK291626.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361926.8/ ENSP00000355026.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-547               DB279954.1         4-550
            548-1086            AB020722.1         467-1005
            1087-2642           BC036749.1         1045-2600
            2643-3037           BQ877184.1         320-714
            3038-4196           BC036749.1         2996-4154
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.1"
     gene            1..4196
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /note="Rho guanine nucleotide exchange factor 15"
                     /db_xref="GeneID:22899"
                     /db_xref="HGNC:HGNC:15590"
                     /db_xref="MIM:608504"
     exon            1..103
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            104..753
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     CDS             153..2678
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /note="ephexin-5; Rho guanine nucleotide exchange factor
                     (GEF) 15"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 15"
                     /protein_id="NP_776089.2"
                     /db_xref="CCDS:CCDS11139.1"
                     /db_xref="GeneID:22899"
                     /db_xref="HGNC:HGNC:15590"
                     /db_xref="MIM:608504"
                     /translation="MSAQSLPAATPPTQKPPRIIRPRPPSRSRAAQSPGPPHNGSSPQ
                     ELPRNSNDAPTPMCTPIFWEPPAASLKPPALLPPSASRASLDSQTSPDSPSSTPTPSP
                     VSRRSASPEPAPRSPVPPPKPSGSPCTPLLPMAGVLAQNGSASAPGTVRRLAGRFEGG
                     AEGRAQDADAPEPGLQARADVNGEREAPLTGSGSQENGAPDAGLACPPCCPCVCHTTR
                     PGLELRWVPVGGYEEVPRVPRRASPLRTSRSRPHPPSIGHPAVVLTSYRSTAERKLLP
                     LLKPPKPTRVRQDATIFGDPPQPDLDLLSEDGIQTGDSPDEAPQNTPPATVEGREEEG
                     LEVLKEQNWELPLQDEPLYQTYRAAVLSEELWGVGEDGSPSPANAGDAPTFPRPPGPR
                     NTLWQELPAVQASGLLDTLSPQERRMQESLFEVVTSEASYLRSLRLLTDTFVLSQALR
                     DTLTPRDHHTLFSNVQRVQGVSERFLATLLSRVRSSPHISDLCDVVHAHAVGPFSVYV
                     DYVRNQQYQEETYSRLMDTNVRFSAELRRLQSLPKCERLPLPSFLLLPFQRITRLRML
                     LQNILRQTEEGSSRQENAQKALGAVSKIIERCSAEVGRMKQTEELIRLTQRLRFHKVK
                     ALPLVSWSRRLEFQGELTELGCRRGGVLFASRPRFTPLCLLLFSDLLLITQPKSGQRL
                     QVLDYAHRSLVQAQQVPDPSGPPTFRLSLLSNHQGRPTHRLLQASSLSDMQRWLGAFP
                     TPGPLPCSPDTIYEDCDCSQELCSESSAPAKTEGRSLESRAAPKHLHKTPEGWLKGLP
                     GAFPAQLVCEVTGEHERRRHLRQNQRLLEAVGSSSGTPNAPPP"
     exon            754..1086
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1141
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1200
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1412
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1573
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1726
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..1856
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1857..1931
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1932..2024
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2025..2185
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2186..2338
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2339..2421
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2422..2526
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2527..4196
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4175..4180
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /note="hexamer: AGTAAA"
     polyA_site      4196
                     /gene="ARHGEF15"
                     /gene_synonym="ARGEF15; E5; Ephexin5; Vsm-RhoGEF"
                     /note="major polyA site"
ORIGIN      
        1 agaatcacag gaacacacac ttggaaacct cagccctgca ttcctcgctc caaggggcag
       61 acaggacagg ctgaaaatag caactggttc caaaaagata aaggggatga ctccagcaga
      121 gcacctcact cctttgaaga gcacagagga agatgtcagc ccagtccctt cctgcagcaa
      181 caccccccac gcagaagccc cctcggatca tccgcccccg ccctccttct cgttccaggg
      241 ctgcccagtc cccagggcct ccccacaatg gctcctctcc acaagaacta ccccgaaact
      301 ccaatgatgc accaacccca atgtgcaccc ccatcttctg ggagccccca gctgcatccc
      361 tcaagccccc tgctcttttg cccccctcag cttctagagc cagcctcgac tcccagactt
      421 ccccagactc accttccagc acccccacac ctagtccagt gtcccggcgc tccgcctccc
      481 cagaacctgc tccccggtct ccagtccccc cacccaagcc gtctgggtca ccctgcacgc
      541 ctctgctccc catggctgga gtcctggctc agaatggctc tgcctcagct cctggcactg
      601 tgcggaggct ggctggcagg tttgaagggg gtgctgaagg ccgggctcag gatgcagatg
      661 ccccggagcc aggtctccaa gcgagagcag atgtgaatgg ggagagagaa gctcccctca
      721 ccgggagtgg gtcccaggag aacggtgctc cagatgctgg cctggcctgc cctccctgct
      781 gcccctgtgt ctgccacacc acccggcctg gcctggagct cagatgggtg cctgtggggg
      841 gctatgagga ggtccccagg gtcccccgtc gggcctcccc gctgcggacc tctcgctccc
      901 gcccccaccc tccaagcatc ggtcaccctg ccgttgtcct cacatcctac cgctccactg
      961 ctgagcgcaa actcctgcca ctcctcaagc ctcccaaacc aactcgtgtc aggcaggatg
     1021 ccaccatttt cggggacccc ccacagccag atcttgatct gctttctgaa gatggaatcc
     1081 aaacagggga cagtcctgat gaagctcctc agaatactcc tccagcaact gtggagggga
     1141 gggaagagga ggggctagag gtgctgaagg agcagaattg ggagctgccc ctgcaggatg
     1201 aacctctgta ccagacctac cgagcagccg tgctgtcaga ggagctgtgg ggggtgggtg
     1261 aggatgggag tccttctcca gcaaatgctg gagatgcacc caccttccca cgaccccctg
     1321 gacctcgcaa caccctgtgg caggagcttc cggctgtgca agccagcggt cttctggata
     1381 ccctcagccc ccaggagagg cgcatgcagg agagtctttt cgaggtggtg acgtccgagg
     1441 cttcctacct gcgctccctg cggctgctga ccgacacctt cgtgctgagc caggcactcc
     1501 gggacacgct caccccccgt gatcaccaca cactcttctc caatgtgcag cgagtccagg
     1561 gagtcagcga gcggtttcta gcaacgctcc tgtcccgtgt gcgctcttcc ccccacatca
     1621 gcgacttgtg tgatgtggtg catgcccacg ctgtggggcc tttctcggtg tatgtggatt
     1681 atgtgcggaa ccagcagtat caggaggaga cctacagccg cctcatggac accaacgtgc
     1741 gcttctccgc cgagctgcgc cggctgcaga gcctccctaa gtgtgagcgg ctcccgctgc
     1801 cgtccttcct gctactgccc ttccagcgca tcacccggct gcgcatgctg ctgcagaata
     1861 tcctgcgcca gacagaagag gggtccagcc gtcaggagaa tgcccagaag gccctgggtg
     1921 ctgtcagcaa gatcatcgag cgttgcagcg ctgaggtggg gcgcatgaag cagactgaag
     1981 agctgatccg gctcacccaa aggctgcgct tccacaaagt caaggccctg cccctggtct
     2041 cctggtcacg gcgcctggaa ttccagggag agctgactga gttagggtgc cggagggggg
     2101 gcgtgctctt tgcctcgcgc ccccgcttca cccctctttg cctgctgctc tttagcgacc
     2161 tgctgctcat cactcagcct aagagtgggc agcggttaca ggttctggac tatgcccatc
     2221 gctccctggt ccaggcccag caggttccgg atccatctgg accccctacc ttccgcctct
     2281 cccttctcag caaccaccag ggccgcccca cccaccgact actccaagct tcttccctat
     2341 cagacatgca gcgctggctg ggagccttcc caaccccagg cccccttccc tgctccccag
     2401 acaccatcta tgaggactgt gactgttccc aggaactgtg ttcagagtcg tctgcacctg
     2461 ccaagactga aggacggagt ctggagtcca gggctgcccc caaacacctg cacaagaccc
     2521 ctgaaggttg gctgaagggg cttcctgggg ccttccctgc ccagctggtg tgtgaagtca
     2581 caggggaaca cgaaaggagg aggcaccttc gccagaacca gaggcttctc gaggctgttg
     2641 gatcttcttc aggcaccccc aatgcccccc caccctaatg caggctgagg agggggcaca
     2701 tgttgggaga cacctaccag tgtggcacgg agagaacaaa gcccattcat ccattggatt
     2761 cactgtcagt ggagatacta cctctcgtgg caaccataga gatcgagctt caggacagag
     2821 cagccaatga aaacggccgc ctgaacccac agcaataaga atgaatgagg atgccttgaa
     2881 tgtgtggcca atggagacag aggcttagtg cagagcagcc aatgggtact gagctggctg
     2941 agcctatggc caatgagtat tcctgctatg ctcagggcca aggaagacaa atctaggtca
     3001 tggcagttga aaaagggcct cattggagat aaagtcgtag gataaaattg ggaacaggaa
     3061 tgagcaggaa gccaatcagc caaagaaaat ggtgatctgg acccaagaga ccagtagtca
     3121 ccctgcttgt ttctgcagca atgactggtc ctgtcttttg agtctgggaa atactagttt
     3181 ccattcctgg atgcttcctg tgccctctca agccagttct tctcttccag aagaattcag
     3241 agtatgtgtc tcagaaaatc tgtgtgtgta catgtgcatg tgtagatatg tgtgtatatg
     3301 tatcaggaaa ggcattctgc tgactgtggt gtgtgtgtgg tgattgtgct cctgacccac
     3361 aaatgactga gtgctccatt tcttccttta ccccccattt ttcctattat tcgctccaag
     3421 aaagatgcta agtctgagct ccagaagaga ctgtgctggg tgtggcttgg cacccagggg
     3481 atgagagccc tgagctttgg gtctcttgga ggctagggtt ctgtggcagt tgcagggcaa
     3541 tgttatggag cagccaacgg cctggcagag gagcccaagg gactgaagat ggccagtagc
     3601 tgggtcctga ggcccctgaa gtctgcagac ccttctcctt gccccaaaca ctggcctcca
     3661 taattcctgc ctgcagatct cccaacttga actataatcc accagccagc ctcagccttg
     3721 agctttggaa ccacattaga tcctgcatct gggtgaagaa acgggagctg tggaccacag
     3781 gccagccagt gaacctcctg ggctttcttg cctttgtcct gatcctctca cagaaacact
     3841 gggccaaaca gtggggagag attggagagc gggtgtggct gcccaacccc atccagagca
     3901 tctgcttcca gatgagccag tgcctcgcat gataccagag gaggcgaggg acagagacag
     3961 caaggcagac agtggctggc aggggggccc aggcccggga cgaggcctcc ccttcagctc
     4021 aggcacagca acttgcccag gactgacact gtcaccctga ctgcaggagg cacagggact
     4081 ccgggagact cagagggcga agagcactgg catttggcat gtccatgaca ttggagactc
     4141 ccctagcagg gtgcctgacg tgtggggaac cctcagtaaa tagtggtgca tttgta
//
