LOCUS       NM_181335               1615 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens Rho GTPase activating protein 8 (ARHGAP8), transcript
            variant 2, mRNA.
ACCESSION   NM_181335
VERSION     NM_181335.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Wong,D.C.P., Pan,C.Q., Er,S.Y., Thivakar,T., Rachel,T.Z.Y.,
            Seah,S.H., Chua,P.J., Jiang,T., Chew,T.W., Chaudhuri,P.K.,
            Mukherjee,S., Salim,A., Aye,T.A., Koh,C.G., Lim,C.T., Tan,P.H.,
            Bay,B.H., Ridley,A.J. and Low,B.C.
  TITLE     The scaffold RhoGAP protein ARHGAP8/BPGAP1 synchronizes Rac and Rho
            signaling to facilitate cell migration
  JOURNAL   Mol Biol Cell 34 (3), ar13 (2023)
   PUBMED   36598812
  REMARK    GeneRIF: The scaffold RhoGAP protein ARHGAP8/BPGAP1 synchronizes
            Rac and Rho signaling to facilitate cell migration.
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1615)
  AUTHORS   McElroy,S.L., Winham,S.J., Cuellar-Barboza,A.B., Colby,C.L.,
            Ho,A.M., Sicotte,H., Larrabee,B.R., Crow,S., Frye,M.A. and
            Biernacka,J.M.
  TITLE     Bipolar disorder with binge eating behavior: a genome-wide
            association study implicates PRR5-ARHGAP8
  JOURNAL   Transl Psychiatry 8 (1), 40 (2018)
   PUBMED   29391396
  REMARK    GeneRIF: Fusion protein PRR5-ARHGAP8 plays a role in bipolar
            disorder with binge eating behavior.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1615)
  AUTHORS   Jiang,T., Pan,C.Q. and Low,B.C.
  TITLE     BPGAP1 spatially integrates JNK/ERK signaling crosstalk in
            oncogenesis
  JOURNAL   Oncogene 36 (22), 3178-3192 (2017)
   PUBMED   28092672
  REMARK    GeneRIF: BPGAP1 provides a crucial spatiotemporal checkpoint where
            JNK and MP1/MEK1 work in concert to regulate endosomal and nuclear
            ERK signaling in cell proliferation control.
REFERENCE   5  (bases 1 to 1615)
  AUTHORS   Winham,S.J., Cuellar-Barboza,A.B., McElroy,S.L., Oliveros,A.,
            Crow,S., Colby,C.L., Choi,D.S., Chauhan,M., Frye,M.A. and
            Biernacka,J.M.
  TITLE     Bipolar disorder with comorbid binge eating history: a genome-wide
            association study implicates APOB
  JOURNAL   J Affect Disord 165, 151-158 (2014)
   PUBMED   24882193
REFERENCE   6  (bases 1 to 1615)
  AUTHORS   Lua,B.L. and Low,B.C.
  TITLE     BPGAP1 interacts with cortactin and facilitates its translocation
            to cell periphery for enhanced cell migration
  JOURNAL   Mol Biol Cell 15 (6), 2873-2883 (2004)
   PUBMED   15064355
  REMARK    GeneRIF: RhoGAP functionally interacts with cortactin and
            represents a novel determinant in the regulation of cell dynamics.
REFERENCE   7  (bases 1 to 1615)
  AUTHORS   Shang,X., Zhou,Y.T. and Low,B.C.
  TITLE     Concerted regulation of cell dynamics by BNIP-2 and Cdc42GAP
            homology/Sec14p-like, proline-rich, and GTPase-activating protein
            domains of a novel Rho GTPase-activating protein, BPGAP1
  JOURNAL   J Biol Chem 278 (46), 45903-45914 (2003)
   PUBMED   12944407
  REMARK    GeneRIF: BPGAP1 has a role in regulating cell dynamics with BNIP-2
            and Cdc42GAP homology/Sec14p-like, proline-rich, and
            GTPase-activating protein domains of BPGAP1
REFERENCE   8  (bases 1 to 1615)
  AUTHORS   Shan,Z., Haaf,T. and Popescu,N.C.
  TITLE     Identification and characterization of a gene encoding a putative
            mouse Rho GTPase activating protein gene 8, Arhgap8
  JOURNAL   Gene 303, 55-61 (2003)
   PUBMED   12559566
  REMARK    GeneRIF: This paper compares the protein sequences and genomic
            structures of human and mouse ARHGAP8.
REFERENCE   9  (bases 1 to 1615)
  AUTHORS   Peck,J., Douglas,G. 4th, Wu,C.H. and Burbelo,P.D.
  TITLE     Human RhoGAP domain-containing proteins: structure, function and
            evolutionary relationships
  JOURNAL   FEBS Lett 528 (1-3), 27-34 (2002)
   PUBMED   12297274
  REMARK    Review article
REFERENCE   10 (bases 1 to 1615)
  AUTHORS   Dunham,I., Shimizu,N., Roe,B.A., Chissoe,S., Hunt,A.R.,
            Collins,J.E., Bruskiewich,R., Beare,D.M., Clamp,M., Smink,L.J.,
            Ainscough,R., Almeida,J.P., Babbage,A., Bagguley,C., Bailey,J.,
            Barlow,K., Bates,K.N., Beasley,O., Bird,C.P., Blakey,S.,
            Bridgeman,A.M., Buck,D., Burgess,J., Burrill,W.D., O'Brien,K.P. et
            al.
  TITLE     The DNA sequence of human chromosome 22
  JOURNAL   Nature 402 (6761), 489-495 (1999)
   PUBMED   10591208
  REMARK    Erratum:[Nature 2000 Apr 20;404(6780):904]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK001306.1, AK022305.1,
            BC010490.2 and Z83838.2.
            
            On May 21, 2020 this sequence version replaced NM_181335.2.
            
            Summary: This gene encodes a member of the RHOGAP family. GAP
            (GTPase-activating) family proteins participate in signaling
            pathways that regulate cell processes involved in cytoskeletal
            changes. GAP proteins alternate between an active (GTP-bound) and
            inactive (GDP-bound) state based on the GTP:GDP ratio in the cell.
            This family member is a multidomain protein that functions to
            promote Erk activation and cell motility. Alternative splicing
            results in multiple transcript variants. Read-through transcripts
            from the upstream proline rich 5, renal (PRR5) gene into this gene
            also exist, which led to the original description of PRR5 and
            ARHGAP8 being a single gene. [provided by RefSeq, Nov 2010].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the central coding reigon, compared to variant 1, resulting
            in an isoform (2) that is shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.4515116.1,
                                           SRR18074967.2784257.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356099.11/ ENSP00000348407.6
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-203               AK001306.1         18-220
            204-291             AK022305.1         163-250
            292-423             AK001306.1         309-440
            424-609             AK001306.1         534-719
            610-720             AK022305.1         569-679
            721-1095            AK001306.1         831-1205
            1096-1407           BC010490.2         957-1268
            1408-1615           Z83838.2           48028-48235
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.31"
     gene            1..1615
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /note="Rho GTPase activating protein 8"
                     /db_xref="GeneID:23779"
                     /db_xref="HGNC:HGNC:677"
                     /db_xref="MIM:609405"
     exon            1..53
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /inference="alignment:Splign:2.1.0"
     exon            54..203
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..1426
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /note="isoform 2 is encoded by transcript variant 2; BCH
                     domain-containing Cdc42GAP-like protein; BNIP-2 and
                     Cdc42GAP homology domain-containing, proline-rich and
                     Cdc42GAP-like protein subtype-1; rho-type
                     GTPase-activating protein 8"
                     /codon_start=1
                     /product="rho GTPase-activating protein 8 isoform 2"
                     /protein_id="NP_851852.2"
                     /db_xref="CCDS:CCDS14060.2"
                     /db_xref="GeneID:23779"
                     /db_xref="HGNC:HGNC:677"
                     /db_xref="MIM:609405"
                     /translation="MAGQDPALSTSHPFYDVARHGILQVAGDDRFGRRVVTFSCCRMP
                     PSHELDHQRLLEYLKYTLDQYVENDYTIVYFHYGLNSRNKPSLGWLQSAYKEFDRKYK
                     KNLKALYVVHPTSFIKVLWNILKPLISHKFGKKVIYFNYLSELHEHLKYDQLVIPPEV
                     LRYDEKLQSLHEGRTPPPTKTPPPRPPLPTQQFGVSLQYLKDKNQGELIPPVLRFTVT
                     YLREKGLRTEGLFRRSASVQTVREIQRLYNQGKPVNFDDYGDIHIPAVILKTFLRELP
                     QPLLTFQAYEQILGITCVESSLRVTGCRQILRSLPEHNYVVLRYLMGFLHAVSRESIF
                     NKMNSSNLACVFGLNLIWPSQGVSSLSALVPLNMFTELLIEYYEKIFSTPEAPGEHGL
                     APWEQGSRAAPLQEAVPRTQATGLTKPTLPPSPLMAARRRL"
     exon            204..291
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /inference="alignment:Splign:2.1.0"
     exon            292..423
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /inference="alignment:Splign:2.1.0"
     exon            424..510
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /inference="alignment:Splign:2.1.0"
     exon            511..609
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /inference="alignment:Splign:2.1.0"
     exon            610..720
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /inference="alignment:Splign:2.1.0"
     exon            721..794
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /inference="alignment:Splign:2.1.0"
     exon            795..872
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /inference="alignment:Splign:2.1.0"
     exon            873..1001
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1105
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1615
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1551
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /note="major polyA site"
     polyA_site      1615
                     /gene="ARHGAP8"
                     /gene_synonym="BPGAP1; PP610"
                     /note="major polyA site"
ORIGIN      
        1 aggtgagcgg cagacccggc acgcaggtgg gggccggcgg ggtccgtggc cagagctgca
       61 gagagacaag gcggcggcgg ctgctgtgct gggtgcagtg aggaagaggc cctcggtggt
      121 gcccatggct ggccaggatc ctgcgctgag cacgagtcac ccgttctacg acgtggccag
      181 acatggcatt ctgcaggtgg caggggatga ccgctttgga agacgtgttg tcacgttcag
      241 ctgctgccgg atgccaccct cccacgagct ggaccaccag cggctgctgg agtatttgaa
      301 gtacacactg gaccaatacg ttgagaacga ttataccatc gtctatttcc actacgggct
      361 gaacagccgg aacaagcctt ccctgggctg gctccagagc gcatacaagg agttcgatag
      421 gaagtacaag aagaacttga aggccctcta cgtggtgcac cccaccagct tcatcaaggt
      481 cctgtggaac atcttgaagc ccctcatcag tcacaagttt gggaagaaag tcatctattt
      541 caactacctg agtgagctcc acgaacacct taaatacgac cagctggtca tccctcccga
      601 agttttgcgg tacgatgaga agctccagag cctgcacgag ggccggacgc cgcctcccac
      661 caagacacca ccgccgcggc ccccgctgcc cacacagcag tttggcgtca gtctgcaata
      721 cctcaaagac aaaaatcaag gcgaactcat cccccctgtg ctgaggttca cagtgacgta
      781 cctgagagag aaaggcctgc gcaccgaggg cctgttccgg agatccgcca gcgtgcagac
      841 cgtccgcgag atccagaggc tctacaacca agggaagccc gtgaactttg acgactacgg
      901 ggacattcac atccctgccg tgatcctgaa gaccttcctg cgagagctgc cccagccgct
      961 tctgaccttc caggcctacg agcagattct cgggatcacc tgtgtggaga gcagcctgcg
     1021 tgtcactggc tgccgccaga tcttacggag cctcccagag cacaactacg tcgtcctccg
     1081 ctacctcatg ggcttcctgc atgcggtgtc ccgggagagc atcttcaaca aaatgaacag
     1141 ctctaacctg gcctgtgtct tcgggctgaa tttgatctgg ccatcccagg gggtctcctc
     1201 cctgagtgcc cttgtgcccc tgaacatgtt cactgaactg ctgatcgagt actatgaaaa
     1261 gatcttcagc accccggagg cacctgggga gcacggcctg gcaccatggg aacaggggag
     1321 cagggcagcc cctttgcagg aggctgtgcc acggacacaa gccacgggcc tcaccaagcc
     1381 taccctacct ccgagtcccc tgatggcagc cagaagacgt ctctagtgtt gcgaacactc
     1441 tgtatatttc gagctacctc ccacacctgt ctgtgcactt gtatgttttg taaacttggc
     1501 atctgtaaaa ataaccagcc attagatgaa ttcagaacct tctaatgaaa actccatgcc
     1561 tctggtcctt ggactcttgt ccatggttcc tgagctgtgg accgggatag aataa
//
