LOCUS       NM_004491               9290 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), mRNA.
ACCESSION   NM_004491 XM_928685 XM_941252
VERSION     NM_004491.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Vish,K.J., Stiegler,A.L. and Boggon,T.J.
  TITLE     Diverse p120RasGAP interactions with doubly phosphorylated partners
            EphB4, p190RhoGAP, and Dok1
  JOURNAL   J Biol Chem 299 (9), 105098 (2023)
   PUBMED   37507023
  REMARK    GeneRIF: Diverse p120RasGAP interactions with doubly phosphorylated
            partners EphB4, p190RhoGAP, and Dok1.
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   Reis,L.M., Chassaing,N., Bardakjian,T., Thompson,S., Schneider,A.
            and Semina,E.V.
  TITLE     ARHGAP35 is a novel factor disrupted in human developmental eye
            phenotypes
  JOURNAL   Eur J Hum Genet 31 (3), 363-367 (2023)
   PUBMED   36450800
  REMARK    GeneRIF: ARHGAP35 is a novel factor disrupted in human
            developmental eye phenotypes.
REFERENCE   3  (bases 1 to 9290)
  AUTHORS   Yagi,H., Onoyama,I., Asanoma,K., Kawakami,M., Maenohara,S.,
            Kodama,K., Matsumura,Y., Hamada,N., Hori,E., Hachisuga,K.,
            Yasunaga,M., Ohgami,T., Okugawa,K., Yahata,H. and Kato,K.
  TITLE     Tumor-derived ARHGAP35 mutations enhance the Galpha13-Rho signaling
            axis in human endometrial cancer
  JOURNAL   Cancer Gene Ther 30 (2), 313-323 (2023)
   PUBMED   36257976
  REMARK    GeneRIF: Tumor-derived ARHGAP35 mutations enhance the Galpha13-Rho
            signaling axis in human endometrial cancer.
REFERENCE   4  (bases 1 to 9290)
  AUTHORS   Heraud,C., Pinault,M., Neaud,V., Saltel,F., Lagree,V. and Moreau,V.
  TITLE     Identification of an inhibitory domain in GTPase-activating protein
            p190RhoGAP responsible for masking its functional GAP domain
  JOURNAL   J Biol Chem 299 (1), 102792 (2023)
   PUBMED   36516886
  REMARK    GeneRIF: Identification of an inhibitory domain in
            GTPase-activating protein p190RhoGAP responsible for masking its
            functional GAP domain.
REFERENCE   5  (bases 1 to 9290)
  AUTHORS   Lippincott,M.F., Xu,W., Smith,A.A., Miao,X., Lafont,A., Shennib,O.,
            Farley,G.J., Sabbagh,R., Delaney,A., Stamou,M., Plummer,L.,
            Salnikov,K., Georgopoulos,N.A., Mericq,V., Quinton,R.,
            Mau-Them,F.T., Nambot,S., Hamad,A., Brittain,H., Tooze,R.S.,
            Calpena,E., Wilkie,A.O.M., Willems,M., Crowley,W.F.,
            Balasubramanian,R., Lamarche-Vane,N., Davis,E.E. and Seminara,S.B.
  TITLE     The p190 RhoGAPs, ARHGAP35, and ARHGAP5 are implicated in GnRH
            neuronal development: Evidence from patients with idiopathic
            hypogonadotropic hypogonadism, zebrafish, and in vitro GAP activity
            assay
  JOURNAL   Genet Med 24 (12), 2501-2515 (2022)
   PUBMED   36178483
  REMARK    GeneRIF: The p190 RhoGAPs, ARHGAP35, and ARHGAP5 are implicated in
            GnRH neuronal development: Evidence from patients with idiopathic
            hypogonadotropic hypogonadism, zebrafish, and in vitro GAP activity
            assay.
REFERENCE   6  (bases 1 to 9290)
  AUTHORS   Rush,J., Moritz,A., Lee,K.A., Guo,A., Goss,V.L., Spek,E.J.,
            Zhang,H., Zha,X.M., Polakiewicz,R.D. and Comb,M.J.
  TITLE     Immunoaffinity profiling of tyrosine phosphorylation in cancer
            cells
  JOURNAL   Nat Biotechnol 23 (1), 94-101 (2005)
   PUBMED   15592455
REFERENCE   7  (bases 1 to 9290)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   8  (bases 1 to 9290)
  AUTHORS   Tikoo,A., Czekay,S., Viars,C., White,S., Heath,J.K., Arden,K. and
            Maruta,H.
  TITLE     p190-A, a human tumor suppressor gene, maps to the chromosomal
            region 19q13.3 that is reportedly deleted in some gliomas
  JOURNAL   Gene 257 (1), 23-31 (2000)
   PUBMED   11054565
REFERENCE   9  (bases 1 to 9290)
  AUTHORS   Warriar,N., Page,N. and Govindan,M.V.
  TITLE     Expression of human glucocorticoid receptor gene and interaction of
            nuclear proteins with the transcriptional control element
  JOURNAL   J Biol Chem 271 (31), 18662-18671 (1996)
   PUBMED   8702520
REFERENCE   10 (bases 1 to 9290)
  AUTHORS   LeClerc,S., Palaniswami,R., Xie,B.X. and Govindan,M.V.
  TITLE     Molecular cloning and characterization of a factor that binds the
            human glucocorticoid receptor gene and represses its expression
  JOURNAL   J Biol Chem 266 (26), 17333-17340 (1991)
   PUBMED   1894621
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008895.7, AB051509.2,
            BE871213.1, CX163309.1, AC008755.8 and CA429690.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 8, 2019 this sequence version replaced NM_004491.4.
            
            Summary: The human glucocorticoid receptor DNA binding factor,
            which associates with the promoter region of the glucocorticoid
            receptor gene (hGR gene), is a repressor of glucocorticoid receptor
            transcription. The amino acid sequence deduced from the cDNA
            sequences show the presence of three sequence motifs characteristic
            of a zinc finger and one motif suggestive of a leucine zipper in
            which 1 cysteine is found instead of all leucines. The GRLF1
            enhances the homologous down-regulation of wild-type hGR gene
            expression. Biochemical analysis suggests that GRLF1 interaction is
            sequence specific and that transcriptional efficacy of GRLF1 is
            regulated through its interaction with specific sequence motif. The
            level of expression is regulated by glucocorticoids. [provided by
            RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments and orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.2616084.1,
                                           SRR1803612.13661.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000672722.1/ ENSP00000500409.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-213               AC008895.7         11176-11388
            214-401             AC008895.7         68667-68854
            402-5920            AB051509.2         1-5519
            5921-6166           BE871213.1         88-333
            6167-6921           CX163309.1         25-779
            6922-8130           AB051509.2         6098-7306
            8131-8660           AC008755.8         30749-31278
            8661-9290           CA429690.1         16-645              c
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.32"
     gene            1..9290
                     /gene="ARHGAP35"
                     /gene_synonym="GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP;
                     p190RhoGAP"
                     /note="Rho GTPase activating protein 35"
                     /db_xref="GeneID:2909"
                     /db_xref="HGNC:HGNC:4591"
                     /db_xref="MIM:605277"
     exon            1..213
                     /gene="ARHGAP35"
                     /gene_synonym="GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP;
                     p190RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            214..4082
                     /gene="ARHGAP35"
                     /gene_synonym="GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP;
                     p190RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             402..4901
                     /gene="ARHGAP35"
                     /gene_synonym="GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP;
                     p190RhoGAP"
                     /note="glucocorticoid receptor DNA-binding factor 1; rho
                     GAP p190A; glucocorticoid receptor repression factor 1"
                     /codon_start=1
                     /product="rho GTPase-activating protein 35"
                     /protein_id="NP_004482.4"
                     /db_xref="CCDS:CCDS46127.1"
                     /db_xref="GeneID:2909"
                     /db_xref="HGNC:HGNC:4591"
                     /db_xref="MIM:605277"
                     /translation="MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVR
                     PSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDD
                     QTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGF
                     LLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVVVLTKCDEGVERYIRDAHTFAL
                     SKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDK
                     YEWLVSRIVKNHNENWLSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIE
                     RRRKLYLAALPLAFEALIPNLDEIDHLSCIKAKKLLETKPEFLKWFVVLEETPWDATS
                     HIDNMENERIPFDLMDTVPAEQLYEAHLEKLRNERKRVEMRRAFKENLETSPFITPGK
                     PWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDKAKEEFQELLLEYSELFYELEL
                     DAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACV
                     DAKIEHLISSRFIRPSDRNQKNSLSDPNIDRINLVILGKDGLARELANEIRALCTNDD
                     KYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSR
                     ESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGI
                     GYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADVDLRIVMCLMCGDPFSADD
                     ILFPVLQSQTCKSSHCGSNNSVLLELPIGLHKKRIELSVLSYHSSFSIRKSRLVHGYI
                     VFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAVDVLDNDLSREQLTEGEEIAQE
                     IDGRFTSIPCSQPQHKLEIFHPFFKDVVEKKNIIEATHMYDNAAEACSTTEEVFNSPR
                     AGSPLCNSNLQDSEEDIEPSYSLFREDTSLPSLSKDHSKLSMELEGNDGLSFIMSNFE
                     SKLNNKVPPPVKPKPPVHFEITKGDLSYLDQGHRDGQRKSVSSSPWLPQDGFDPSDYA
                     EPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDTSSLERG
                     RKVSIVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKGDNAV
                     IPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKPIP
                     IFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGA
                     MKSFFSELPDPLVPYNMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISH
                     LNKVSHNNKVNLMTSENLSICFWPTLMRPDFSTMDALTATRTYQTIIELFIQQCPFFF
                     YNRPITEPPGARPSSPSAVASTVPFLTSTPVTSQPSPPQSPPPTPQSPMQPLLPSQLQ
                     AEHTL"
     exon            4083..4227
                     /gene="ARHGAP35"
                     /gene_synonym="GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP;
                     p190RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4228..4305
                     /gene="ARHGAP35"
                     /gene_synonym="GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP;
                     p190RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4306..4437
                     /gene="ARHGAP35"
                     /gene_synonym="GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP;
                     p190RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4438..4543
                     /gene="ARHGAP35"
                     /gene_synonym="GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP;
                     p190RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4544..9290
                     /gene="ARHGAP35"
                     /gene_synonym="GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP;
                     p190RhoGAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8748..8753
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGAP35"
                     /gene_synonym="GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP;
                     p190RhoGAP"
                     /note="hexamer: AATAAA"
     polyA_site      8773
                     /gene="ARHGAP35"
                     /gene_synonym="GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP;
                     p190RhoGAP"
     regulatory      9268..9273
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGAP35"
                     /gene_synonym="GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP;
                     p190RhoGAP"
                     /note="hexamer: AATGAA"
     polyA_site      9290
                     /gene="ARHGAP35"
                     /gene_synonym="GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP;
                     p190RhoGAP"
                     /note="major polyA site"
ORIGIN      
        1 gccccgaggg agagccgcgg cgcggcggca ggaggaggtg gaggaggcgg aggagggaac
       61 ccgcgaggga gggtccgccc ggccccccgc cgccggagcc gccgccgccg cctcagccgc
      121 cgctggacta ggagcagggg aacatggcgc cggggccccc gtcgttgctg ccgggatttt
      181 ggaggccggg gccgcgctga gggcgcccag ctggaagaaa tgttggctat ttggcgatga
      241 gaggtggtga acagtgacca tactggtata caacactatg ggacctggca tttttgctgc
      301 atgtccagcc cacccccact aataatgtag gaagctgtct ggtccattgg aaacactaat
      361 ctgatctcag aagtggctga tcgtggcagg atgtgtcgac gatgatgatg gcaagaaagc
      421 aagatgtccg aattcccacc tacaacatca gtgtggtggg attatctggg accgagaagg
      481 aaaagggcca gtgtgggatt ggaaagtctt gtttgtgcaa ccgcttcgtg cgcccgagtg
      541 ctgacgagtt tcacttggac catacctccg tcctcagcac cagtgacttt ggagggcgag
      601 tggtcaataa tgaccacttt ctctactggg gagaagttag ccgctccctg gaggattgtg
      661 tggaatgtaa gatgcacatt gtggagcaga ctgaatttat tgatgatcag acttttcaac
      721 ctcatcgaag cacggccctg cagccctata tcaagagagc tgctgcgacc aagcttgcat
      781 cagctgaaaa actcatgtac ttttgcactg accagctggg gctggagcag gactttgagc
      841 agaaacaaat gccagacgga aagctgctgg ttgatggttt tcttcttggt attgatgtta
      901 gcaggggcat gaataggaac tttgatgacc agctcaagtt tgtctccaat ctctacaatc
      961 agcttgcaaa aacaaaaaag cccatagtgg tggtcctgac taagtgtgac gaaggtgttg
     1021 agcggtacat tagagatgca catacttttg ccttaagcaa aaagaacctc caggttgtgg
     1081 agacctcagc gagatccaat gtaaacgtgg acttggcttt cagcacctta gtgcaactca
     1141 ttgataaaag tcggggaaag acaaaaatca ttccttattt tgaagctctc aagcagcaga
     1201 gtcagcagat agctacagca aaagacaagt atgagtggct ggtgagtcgc attgtgaaaa
     1261 accacaatga gaactggctg agtgtcagcc gaaagatgca ggcctctcca gaataccagg
     1321 actatgtcta cctggaaggg actcagaaag ccaagaagct gtttctacag cacatccacc
     1381 gcctcaagca tgagcatatc gagcgtagga gaaagctgta cctggcagcc ctgccattag
     1441 cttttgaagc tcttatacct aatctagatg aaatagacca cctaagctgc ataaaagcca
     1501 aaaagctctt agaaaccaag ccagaattct tgaagtggtt tgttgtgctt gaagagaccc
     1561 catgggatgc caccagtcac attgacaaca tggaaaacga acggattccc tttgatttaa
     1621 tggataccgt ccctgcagag cagctatacg aggcccactt agagaagctg aggaacgaaa
     1681 ggaaaagagt tgagatgcga agggcgttta aagaaaacct ggagacttct cctttcataa
     1741 ctcccggaaa gccttgggaa gaggcccgta gttttattat gaatgaggat ttctaccagt
     1801 ggctggagga atctgtatac atggatattt atggcaaaca ccaaaagcaa attatagata
     1861 aagcaaagga agaatttcag gagttgcttt tggaatattc agaattgttt tatgaactgg
     1921 agctggatgc taagcccagc aaggagaaga tgggtgttat tcaggatgtt ctgggagagg
     1981 aacagcgatt taaagcatta caaaagctcc aagcagagcg tgatgccctt attctgaaac
     2041 acattcattt tgtgtaccac ccaacaaagg agacatgccc cagctgccca gcttgtgtgg
     2101 acgctaagat tgagcacttg attagttctc ggtttatccg gccgtctgac cggaatcaga
     2161 aaaattcact ctctgaccct aacattgata gaatcaactt ggttatattg ggcaaagacg
     2221 gccttgcccg agagttggcc aatgagattc gagctctttg tacaaatgat gacaagtatg
     2281 tgatagatgg taaaatgtat gagctttccc tgaggccaat agaggggaat gtcaggcttc
     2341 ctgtgaactc tttccagacg ccaacatttc agccccacgg ctgtctctgc ctttacaatt
     2401 caaaggaatc gctatcctat gtagtggaaa gtatagagaa gagtagagag tccacgctgg
     2461 gccggcggga taatcattta gtccatctcc cccttacatt aattttggtt aacaagagag
     2521 gagacaccag tggagagact ctgcatagct taatacagca aggtcaacaa attgctagca
     2581 aacttcagtg tgtctttctc gaccctgctt ctgctggcat tggttacgga cgcaacatta
     2641 atgaaaagca aatcagtcaa gttttgaagg gactcctgga ctctaagcgt aacttaaacc
     2701 tggtcagttc tactgctagc atcaaagatt tggctgatgt tgatctgcga attgttatgt
     2761 gtctgatgtg tggagatcct tttagtgcag atgacatact ttttcctgtc cttcagtccc
     2821 aaacctgtaa atcttcccat tgtggaagca acaactctgt tttacttgaa ctaccaatcg
     2881 gactgcacaa gaagcggatt gaactgtctg ttctttcata ccattcctcc tttagcatca
     2941 gaaagagccg gttggttcat gggtacattg ttttttattc agccaaacgt aaggcctctt
     3001 tggctatgtt acgtgccttt ctttgtgaag tgcaggatat tatccctatt cagcttgtag
     3061 cactcactga tggcgctgta gatgtcctgg acaatgactt aagtagggaa cagctaactg
     3121 agggggagga gattgctcaa gaaattgacg gaaggttcac aagcatcccc tgtagccaac
     3181 cccagcataa acttgagatc tttcacccat tttttaaaga tgtggtggaa aaaaagaaca
     3241 taatcgaggc tactcatatg tacgataatg ctgccgaggc ctgtagcacc accgaagagg
     3301 tgtttaactc cccccgggca ggatcaccgc tctgcaactc aaacctgcag gattcagaag
     3361 aagatatcga gccatcttac agcctgtttc gagaagacac atcactgcct tctctgtcca
     3421 aagaccattc taagctctct atggaactgg agggaaatga tgggctgtct ttcattatga
     3481 gcaattttga gagtaaactg aacaacaaag tacctccgcc agtcaaacca aagcctcctg
     3541 tccattttga aattacaaag ggggatctat cttatttaga ccaaggccat agggatggac
     3601 agaggaagtc tgtgtcttct agcccctggc tgcctcagga tgggtttgat ccttctgact
     3661 atgctgaacc catggatgct gtggtgaagc caaggaatga agaagaaaac atatactccg
     3721 tgccccatga cagcacccaa ggcaaaatca tcaccattcg gaatatcaac aaagcccagt
     3781 ccaacggcag cgggaatggt tctgacagtg aaatggacac cagctctcta gagcgagggc
     3841 gcaaggtttc catcgtgagc aagccagtgc tgtacaggac gagatgcacc cggctggggc
     3901 ggtttgctag ttaccggacc agcttcagcg tggggagtga tgatgagctg gggcccatcc
     3961 ggaagaaaga ggaggatcag gcatcccagg gttataaagg ggacaatgct gtcattccat
     4021 acgaaacaga cgaagacccg cggaggagga atattcttcg cagcctaagg aggaacacta
     4081 agaaaccaaa gcccaaaccc cggccatcca tcacaaaggc aacctgggag agtaactatt
     4141 ttggggtgcc cttaacaact gtcgtgactc cagagaagcc gatccccatt tttattgaaa
     4201 gatgtattga gtacattgaa gccacaggac tgagcacgga aggcatctac cgggtcagcg
     4261 ggaacaagtc tgagatggag agtctgcaga gacagtttga tcaagaccac aacctggacc
     4321 tggcagagaa agactttacg gtgaataccg tggctggtgc catgaagagc tttttctcag
     4381 aactgcctga ccccctggtc ccgtataaca tgcagatcga cttggtggaa gcacacaaaa
     4441 tcaacgaccg ggagcagaag ttgcatgccc ttaaggaggt attaaagaaa tttccaaagg
     4501 aaaaccacga agtcttcaag tatgtcatct ctcacctaaa caaggtcagc cacaacaaca
     4561 aggtgaatct catgaccagc gagaacctct ccatctgctt ctggcccacc ttgatgagac
     4621 ctgatttcag cactatggac gccctcacag ccacgcgcac ctaccagaca atcattgaac
     4681 tctttatcca gcagtgcccc ttcttcttct acaatcggcc catcaccgag ccccccggcg
     4741 ccaggcccag ctccccctct gccgtggctt ccaccgtccc cttcctcact tccacgcctg
     4801 tcacaagtca gccgtcgccc ccacagtcgc ctccacccac cccccagtcc ccaatgcagc
     4861 cactgcttcc ctcccagctt caagccgaac acacgctgtg agccaccaag acctggggcg
     4921 acaggagaac cggtcctctc tctgacgggg tggcatttgg ccttgaacaa aaccaagtcc
     4981 actggggaca gaggcagggg caagtggctc tccccattac cttctcaaga cctcagtggg
     5041 agcaccagcc aatggtacca tcggctgggc tgccaggtac cctgggcctg gcgctgcaga
     5101 cctgagctgg cttggaccca tttgaggact gaactaggca ggcaatggct ccagtgccct
     5161 ccctctgttc cctggaccac caccccacgt agctgctcac accagcctcc gggtgcctcc
     5221 ctctgcttgt acagagccca tggtcgggac agtgccctgg cctttgccgg ggaggaggat
     5281 gctctgagat tcagggtggg gctggcaacc cctgaagaga acacttcctg ttggtctgtc
     5341 tcttcccacc ttccatctgc acacaccccc aaggtaaggg tacagcccgg ctggcggcct
     5401 ccttgggaac gtgtaggcca cggctctgcc accactaggt acctgctgag ggcgctggct
     5461 ctgcagatca gaacaacgga ggatagcttt gtgcctggac ccagagagtg tgggactccc
     5521 cgcttcatcc ccaccgtccc actccacagc cttcccgaaa cattccctgg caaacaaagg
     5581 aacactagga gaaaaaatgg aaaaaccctt ccagtaatta aaaaggaaga aaccacagaa
     5641 agaaaactac agacctcaag attccactct gtgcccgcct ctgccgggag ggagggaggc
     5701 acacaggtgg agctgaccct cgtctttgtg gcagcaaaac caggatgcct ggagctgtgg
     5761 cctgagggcc tgctggggtc ccactcaccc acttaggtct agtcgctaga tcccccgttt
     5821 tcccaagaag agggttcgag cccttggtgg ggacagctgg ggagatggca gtgcaggctg
     5881 gaacctgggc tgccccagaa cacagtccat tacgatagaa acactaattg agcatgtgcg
     5941 tggggtgggg gtgtgtgtgc acatgtgagt gtgagtgtgt gtgggcgctt ggtggggggt
     6001 tggggacagc tggaaggtgc caggtgcact tggggttggg gttggtgtgt tgggtgttga
     6061 agtggaatcg tttcatccca gccatggagg ccaccagcag gagtgttcat ggggatgtgg
     6121 gcgaggtggg gcactttgaa ggaatggcgg tctgctggtg ccctcgaagg ggcatccttc
     6181 ctggtcttcg ctgacccaga ggcgctgtgc ctgcatatca tccaccacca ccctagccca
     6241 gccttcccac tgccccagga aaagctcttc tcctggccac ctctgccccc cagcacctca
     6301 aacttgcatg gctgggctgt ggcctctgcg gccaggaagc ctgacactag gcacccccca
     6361 ggcgagagct agtggggtgc agagggcccc atgccagaca gcccttgggg ctcgttgcac
     6421 tttaagaaat aggatctgtg gtgtattcca gggggcctga tggacacctt tcccgggcgt
     6481 ctgcagctgc cctgcccgtg cccgcctgca gtggttggag acgggagtgg cccttcggct
     6541 cccgagctcc ctctggggac ggctggctca ctgtctccag ttctcaatgg ccaacgaagg
     6601 tgcttggaaa cacctaacct tgcaagtttt accgcctttt gaggaacaca aatcggagaa
     6661 caaacccagg gttcaggcgt gttttctgtg aatgttggat gatgaatttt tgtctcttct
     6721 ggtggagctg tgcctggccc tgtaggccca gggttggctg gaaggtgaca tctgtgtttc
     6781 gttttagctg aggttggcag aaacgttccc aaactccccc agccctggac cccagcagat
     6841 gaggaaacgg ccccatttac tgaccccgcc cccttttcga ggttatgctc acctggtcag
     6901 ctcctcacgt aattgggggt ggagggaaag catggtggtg ccctgggccg tccctgtgtg
     6961 aacgcaggca aaagcagccc agtccccctc actgcttgag ctaacactgc cacctctttt
     7021 gtgtgagcac aaaagccacg tcccaagcca cctggcccga ttccacagat gtatgtgcgg
     7081 ccagtgactt ccccaggagt gtggaggggg tggtgaggag gagcacctgg gctctctacc
     7141 cctctcctca cagaagtacc tgaaactagg tctggggcac tcccaatgca gcgccttgtc
     7201 agccaaggtg ggcaggcagg gactgtggca gcttatgtcc aaagggagcc cccatgcaca
     7261 ggaagccaca gggttcctct tgtttccccc gctaacttca gcctctcatc tgctgctccg
     7321 ggctgaggga ctagaggaca tctcggtcgt ttgaggggca tggccagtcg tggcaggccg
     7381 gccttcagcg tccggtcagg gaagcgtgca gcccaaatgg gcacttgcat gggagccaca
     7441 gaggagcgtc cctggggatt gttgggacca tgctgccccc actcccgctt ttgttggggc
     7501 tctaagttct ggaaggtgtg tgcacagagg gtgctcatgg gactcgcatg cagctctcag
     7561 cactgggtgg gagggcgttg gcttgtccag aatggggacg tggggcagcc acccctgccc
     7621 agcgagagcg cagacaccgt gtgaggggac agcagccctt ggtgcaaagc cagagactga
     7681 tcctggctct gacggctgaa gagggaagac ccaaggctgg gtggcgtggc tcgtgaatcc
     7741 acttagaatt cttggcttgt gtcgcatact gggtgtcacg gcacacattt actctgcatt
     7801 gtccccgtct ttcccatcgc ctagcgtttg gggaggaaca gggagagagc ttcggggcgt
     7861 ctgtctccgt gctctcctgc ctccaccgcc ttggttttgc ttcctgctgg aggcagggca
     7921 cctgctgcga cccagattct tctgcaggat gtgtctgtct ttgtcacggt ggacagaggg
     7981 tgacatcata ggagcagctc gctggccaga aggggatggg ggcatccctg tgcctcactc
     8041 agctcctgct gctcttaggg aaaggaggcc tgggtcaagc cagcatcccc ttggtaaaga
     8101 cccccgcagg ccaccaggca ttctggacac gcacacacac acacacacac acacacacac
     8161 acaaaacttc acagcaggcc agctgcagtg acttgtcatc aagagtcacc tcagctgcgc
     8221 ccccctccca tcctttccta tgagaagcca ctgctttggg ggcgccggct agaaaaagta
     8281 gggtgcggtg gccaggaggg cccctgccgc gcggggggct gggtctggtt gagtcgctgc
     8341 tttcccgagg gcagcgcagg gatccgggga agctgcggca gggagcgggc gccggcttcg
     8401 tggctctgag gtgtaacggg ggtgggctcc ctccctcgga ggacatcgtc tgtgtccagg
     8461 tcagaaagtg gcccaggaag ggggcagttt ctgtcgcggg tccggtgggg gcgcggccgc
     8521 ggtgcggtcg gtgcagcgtg gccaatgcgc ggcgcgcgcg ggggacagag caggaggcgg
     8581 tctgtcacct cggccactgc tgacctgggc tggcctcccc cagccctccc gtggcggagc
     8641 cggcagcgat gctacaggcc taagttattg tttgcataaa aagaatcatg ttccctgtgt
     8701 acatttaaga aaaaaacaaa aaaacggaaa tgtcagaatt gtatggaaat aaaacttgtt
     8761 tgaaaatttg gaatagtgct gctgccagct tatttttctg gtacttgtat tttcacatgt
     8821 taaatgatct ttatatatgt tgaattaaca aatattttga gtttctgaga aaaaacaaaa
     8881 catattaatg gtattgaaat gtgttagtag tctggctgtg tgcccaaaat tctgtttcgc
     8941 agcaaaagtg aagacctgta tgtaaagaaa gtataacaat tatttctttg tattttaggg
     9001 gctttaaccg gaacatcgtc tagctggtgt taggaatgtt tgcttaattt ccagactttt
     9061 ttttaaaaac acatcgtggg ttttttgagg ctccaacctg attagtgcat ggtcagccct
     9121 caatgaaggc tgaggcatct ctgactgagg tgtttttgtt tggttttgtt ttttaaaatc
     9181 atgtatttgc tacaaagtat tgtacttgtc tcaatgggaa tggtgtaaaa aacaaaaggc
     9241 cttatgtgat ctgtatcata gttaataaat gaatcttgta aaaaaccaaa
//
