LOCUS       NM_001378024           10280 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens Rho GTPase activating protein 32 (ARHGAP32),
            transcript variant 3, mRNA.
ACCESSION   NM_001378024 XM_005271736
VERSION     NM_001378024.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10280)
  AUTHORS   Akaishi,Y., Nawa,N., Kashimada,A., Itsui,Y., Okada,E. and
            Yamawaki,M.
  TITLE     Association between Grit and depressive symptoms at the timing of
            job start among medical residents during the COVID-19 pandemic in
            Japan: a cross-sectional study
  JOURNAL   Med Educ Online 28 (1), 2225886 (2023)
   PUBMED   37343594
  REMARK    GeneRIF: Association between Grit and depressive symptoms at the
            timing of job start among medical residents during the COVID-19
            pandemic in Japan: a cross-sectional study.
REFERENCE   2  (bases 1 to 10280)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   3  (bases 1 to 10280)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   4  (bases 1 to 10280)
  AUTHORS   Wang,Y., Pei,W. and Lu,P.
  TITLE     Circ_ARHGAP32 acts as miR-665 sponge to upregulate FGF2 to promote
            ox-LDL induced vascular smooth muscle cells proliferation and
            migration
  JOURNAL   Clin Hemorheol Microcirc 82 (2), 169-182 (2022)
   PUBMED   35662113
  REMARK    GeneRIF: Circ_ARHGAP32 acts as miR-665 sponge to upregulate FGF2 to
            promote ox-LDL induced vascular smooth muscle cells proliferation
            and migration.
REFERENCE   5  (bases 1 to 10280)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 10280)
  AUTHORS   Okabe,T., Nakamura,T., Nishimura,Y.N., Kohu,K., Ohwada,S.,
            Morishita,Y. and Akiyama,T.
  TITLE     RICS, a novel GTPase-activating protein for Cdc42 and Rac1, is
            involved in the beta-catenin-N-cadherin and N-methyl-D-aspartate
            receptor signaling
  JOURNAL   J Biol Chem 278 (11), 9920-9927 (2003)
   PUBMED   12531901
  REMARK    GeneRIF: RICS may regulate dendritic spine morphology and strength
            by modulating Rho GTPases
REFERENCE   7  (bases 1 to 10280)
  AUTHORS   Moon,S.Y., Zang,H. and Zheng,Y.
  TITLE     Characterization of a brain-specific Rho GTPase-activating protein,
            p200RhoGAP
  JOURNAL   J Biol Chem 278 (6), 4151-4159 (2003)
   PUBMED   12454018
  REMARK    GeneRIF: involvement in the regulation of neurite outgrowth by
            exerting its RhoGAP activity and that its cellular activity may be
            regulated through interaction with Src-like tyrosine kinases
REFERENCE   8  (bases 1 to 10280)
  AUTHORS   Gardiner,E.M., Pestonjamasp,K.N., Bohl,B.P., Chamberlain,C.,
            Hahn,K.M. and Bokoch,G.M.
  TITLE     Spatial and temporal analysis of Rac activation during live
            neutrophil chemotaxis
  JOURNAL   Curr Biol 12 (23), 2029-2034 (2002)
   PUBMED   12477392
  REMARK    GeneRIF: This article analyzes Rac activation during live
            neutrophil chemotaxis.
REFERENCE   9  (bases 1 to 10280)
  AUTHORS   Nakamura,T., Komiya,M., Sone,K., Hirose,E., Gotoh,N., Morii,H.,
            Ohta,Y. and Mori,N.
  TITLE     Grit, a GTPase-activating protein for the Rho family, regulates
            neurite extension through association with the TrkA receptor and
            N-Shc and CrkL/Crk adapter molecules
  JOURNAL   Mol Cell Biol 22 (24), 8721-8734 (2002)
   PUBMED   12446789
  REMARK    GeneRIF: These results suggest that Grit, a novel TrkA-interacting
            protein, regulates neurite outgrowth by modulating the Rho family
            of small GTPases.
REFERENCE   10 (bases 1 to 10280)
  AUTHORS   Nakayama,M., Kikuno,R. and Ohara,O.
  TITLE     Protein-protein interactions between large proteins: two-hybrid
            screening using a functionally classified library composed of long
            cDNAs
  JOURNAL   Genome Res 12 (11), 1773-1784 (2002)
   PUBMED   12421765
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP002886.3, AP000747.4 and AP000751.4.
            
            On Jan 24, 2020 this sequence version replaced XM_005271736.4.
            
            Summary: RICS is a neuron-associated GTPase-activating protein that
            may regulate dendritic spine morphology and strength by modulating
            Rho GTPase (see RHOA; MIM 165390) activity (Okabe et al., 2003
            [PubMed 12531901]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (3) represents the longest
            transcript and encodes the longest isoform (3).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000682385.1/ ENSP00000507720.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-243               AP002886.3         46818-47060         c
            244-352             AP002886.3         19054-19162         c
            353-444             AP000747.4         52048-52139         c
            445-486             AP000747.4         51133-51174         c
            487-571             AP000747.4         50691-50775         c
            572-658             AP000747.4         20866-20952         c
            659-796             AP000751.4         121743-121880       c
            797-889             AP000751.4         119853-119945       c
            890-1012            AP000751.4         118914-119036       c
            1013-1090           AP000751.4         117292-117369       c
            1091-1172           AP000751.4         95940-96021         c
            1173-1322           AP000751.4         53331-53480         c
            1323-1425           AP000751.4         43035-43137         c
            1426-1570           AP000751.4         41536-41680         c
            1571-1653           AP000751.4         41015-41097         c
            1654-1761           AP000751.4         36841-36948         c
            1762-1907           AP000751.4         36428-36573         c
            1908-2103           AP000751.4         35565-35760         c
            2104-2249           AP000751.4         33782-33927         c
            2250-2321           AP000751.4         31575-31646         c
            2322-3200           AP000751.4         29136-30014         c
            3201-4180           AP000751.4         27465-28444         c
            4181-10280          AP000751.4         20072-26171         c
FEATURES             Location/Qualifiers
     source          1..10280
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q24.3"
     gene            1..10280
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /note="Rho GTPase activating protein 32"
                     /db_xref="GeneID:9743"
                     /db_xref="HGNC:HGNC:17399"
                     /db_xref="MIM:608541"
     exon            1..243
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..6433
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /note="isoform 3 is encoded by transcript variant 3;
                     RhoGAP involved in the -catenin-N-cadherin and NMDA
                     receptor signaling; brain-specific Rho GTP-ase-activating
                     protein; rho/Cdc42/Rac GTPase-activating protein RICS; rac
                     GTPase activating protein; GAB-associated CDC42; rho
                     GTPase-activating protein 32; GTPase regulator interacting
                     with TrkA; brain-specific Rho GTPase-activating protein;
                     GAB-associated Cdc42/Rac GTPase-activating protein; rhoGAP
                     involved in the beta-catenin-N-cadherin and NMDA receptor
                     signaling; rho-type GTPase-activating protein 32;
                     GTPase-activating protein for Cdc42 and Rac1"
                     /codon_start=1
                     /product="rho GTPase-activating protein 32 isoform 3"
                     /protein_id="NP_001364953.1"
                     /db_xref="CCDS:CCDS91623.1"
                     /db_xref="GeneID:9743"
                     /db_xref="HGNC:HGNC:17399"
                     /db_xref="MIM:608541"
                     /translation="METESESSTLGDDSVFWLESEVIIQVTDCEEEEREEKFRKMKSS
                     VHSEEDDFVPELHRNVHPRERPDWEETLSAMARGADVPEIPGDLTLKTCGSTASMKVK
                     HVKKSTTPGLMGCDNIHRLPFTKGHFPKMAECAHFHYENVEFGSIQLSLSEEQNEVMK
                     NGCESKELVYLVQIACQGKSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDTL
                     KDSPESVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINTPAVG
                     AAHVIKRYTARAPDELTLEVGDIVSVIDMPPKVLSTWWRGKHGFQVGLFPGHCVELIN
                     QKVPQSVTNSVPKPVSKKHGKLITFLRTFMKSRPTKQKLKQRGILKERVFGCDLGEHL
                     LNSGFEVPQVLQSCTAFIERYGIVDGIYRLSGVASNIQRLRHEFDSEHVPDLTKEPYV
                     QDIHSVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLIKIHDVIQQLPPPHY
                     RTLEFLMRHLSLLADYCSITNMHAKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQ
                     SVVVEFILNHVDVLFSGRISMAMQEGAASLSRPKSLLVSSPSTKLLTLEEAQARTQAQ
                     VNSPIVTENKYIEVGEGPAALQGKFHTIIEFPLERKRPQNKMKKSPVGSWRSFFNLGK
                     SSSVSKRKLQRNESEPSEMKAMALKGGRAEGTLRSAKSEESLTSLHAVDGDSKLFRPR
                     RPRSSSDALSASFNGEMLGNRCNSYDNLPHDNESEEEGGLLHIPALMSPHSAEDVDLS
                     PPDIGVASLDFDPMSFQCSPPKAESECLESGASFLDSPGYSKDKPSANKKDAETGSSQ
                     CQTPGSTASSEPVSPLQEKLSPFFTLDLSPTEDKSSKPSSFTEKVVYAFSPKIGRKLS
                     KSPSMSISEPISVTLPPRVSEVIGTVSNTTAQNASSSTWDKCVEERDATNRSPTQIVK
                     MKTNETVAQEAYESEVQPLDQVAAEEVELPGKEDQSVSSSQSKAVASGQTQTGAVTHD
                     PPQDSVPVSSVSLIPPPPPPKNVARMLALALAESAQQASTQSLKRPGTSQAGYTNYGD
                     IAVATTEDNLSSSYSAVALDKAYFQTDRPAEQFHLQNNAPGNCDHPLPETTATGDPTH
                     SNTTESGEQHHQVDLTGNQPHQAYLSGDPEKARITSVPLDSEKSDDHVSFPEDQSGKN
                     SMPTVSFLDQDQSPPRFYSGDQPPSYLGASVDKLHHPLEFADKSPTPPNLPSDKIYPP
                     SGSPEENTSTATMTYMTTTPATAQMSTKEASWDVAEQPTTADFAAATLQRTHRTNRPL
                     PPPPSQRSAEQPPVVGQVQAATNIGLNNSHKVQGVVPVPERPPEPRAMDDPASAFISD
                     SGAAAAQCPMATAVQPGLPEKVRDGARVPLLHLRAESVPAHPCGFPAPLPPTRMMESK
                     MIAAIHSSSADATSSSNYHSFVTASSTSVDDALPLPLPVPQPKHASQKTVYSSFARPD
                     VTTEPFGPDNCLHFNMTPNCQYRPQSVPPHHNKLEQHQVYGARSEPPASMGLRYNTYV
                     APGRNASGHHSKPCSRVEYVSSLSSSVRNTCYPEDIPPYPTIRRVQSLHAPPSSMIRS
                     VPISRTEVPPDDEPAYCPRPLYQYKPYQSSQARSDYHVTQLQPYFENGRVHYRYSPYS
                     SSSSSYYSPDGALCDVDAYGTVQLRPLHRLPNRDFAFYNPRLQGKSLYSYAGLAPRPR
                     ANVTGYFSPNDHNVVSMPPAADVKHTYTSWDLEDMEKYRMQSIRRESRARQKVKGPVM
                     SQYDNMTPAVQDDLGGIYVIHLRSKSDPGKTGLLSVAEGKESRHAAKAISPEGEDRFY
                     RRHPEAEMDRAHHHGGHGSTQPEKPSLPQKQSSLRSRKLPDMGCSLPEHRAHQEASHR
                     QFCESKNGPPYPQGAGQLDYGSKGIPDTSEPVSYHNSGVKYAASGQESLRLNHKEVRL
                     SKEMERPWVRQPSAPEKHSRDCYKEEEHLTQSIVPPPKPERSHSLKLHHTQNVERDPS
                     VLYQYQPHGKRQSSVTVVSQYDNLEDYHSLPQHQRGVFGGGGMGTYVPPGFPHPQSRT
                     YATALGQGAFLPAELSLQHPETQIHAE"
     exon            244..352
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            353..444
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            445..486
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            487..571
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            572..658
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            659..796
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            797..889
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1012
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1090
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..1172
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1322
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1425
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1570
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1653
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..1761
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1907
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            1908..2103
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            2104..2249
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            2250..2321
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            2322..3200
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            3201..4180
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     exon            4181..10280
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      10280
                     /gene="ARHGAP32"
                     /gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS;
                     RICS"
                     /note="major polyA site"
ORIGIN      
        1 accagtacct gtcactaggg caccagacac acatctgagt atacatgagt agagctgaac
       61 aacctgaatg ttggtgcttt ttattccatg cctggaggtt tagttttttt tgttttttaa
      121 gtacaagatg gagactgaaa gtgagagtag cactttaggg gatgacagtg tcttctggtt
      181 ggagtctgaa gttataatcc aggtgactga ctgtgaagag gaagaaaggg aagagaagtt
      241 caggaagatg aagtcctcag tacattctga agaagatgat tttgttccag agctacatag
      301 aaatgtacac cctcgagagc ggcctgattg ggaagaaact cttagcgcaa tggcaagagg
      361 cgcagatgtt ccagagattc ctggagatct tactcttaag acgtgtggca gtacagccag
      421 tatgaaggtt aagcatgtga aaaagtccac cactccagga ctgatgggct gtgacaacat
      481 tcacaggtta cccttcacta aaggtcactt tccgaagatg gctgaatgtg cgcatttcca
      541 ttatgagaat gttgagttcg gcagcataca gctttcactt tcagaagaac agaatgaagt
      601 aatgaaaaat ggctgtgaat ctaaagagct ggtctacctc gtgcagattg cttgtcaggg
      661 aaaaagttgg attgttaaaa gaagttatga agattttcgg gtacttgata aacatcttca
      721 tctgtgtatt tatgaccgaa gattttccca gctctcagaa cttccccgtt ctgacaccct
      781 gaaggacagt ccagagtcgg tcactcagat gcttatggct tacctgtcac gcctttcagc
      841 tatcgctggc aacaagatca actgtgggcc cgcccttacc tggatggaga ttgataataa
      901 gggaaatcac cttttggttc atgaggagtc atccatcaac actcctgctg tcggtgctgc
      961 ccatgttatc aagaggtaca ctgctcgggc ccctgacgaa ctgaccttag aggtgggaga
     1021 cattgtttct gttattgaca tgcccccgaa agtgttaagc acatggtgga gaggcaagca
     1081 cggattccag gtgggactct tccctggaca ctgtgttgag ttaattaacc aaaaagttcc
     1141 ccagtcagtg accaactcag tgccaaaacc agtgtctaaa aagcacggca agctcattac
     1201 gttcttacga acattcatga agtctcgtcc aacaaaacag aagctgaagc agcggggaat
     1261 cttgaaagag agggtgtttg gttgtgacct gggggaacac cttctaaatt ctggttttga
     1321 agtgccgcag gttcttcaaa gctgcacagc attcattgag agatatggca tcgtggatgg
     1381 aatctatcgc ctttctggtg ttgcctccaa tatccagaga ctacgccatg aatttgactc
     1441 tgagcacgtc cccgacctga cgaaagaacc gtatgttcag gacatccatt ctgtgggttc
     1501 cctatgtaag ctgtacttcc gggaactccc aaaccctctg cttacctacc agctgtatga
     1561 gaaattttct gatgcagttt cagcagcaac agatgaagaa aggctgataa aaatccacga
     1621 tgtcatccag cagctccccc caccacacta cagaacactg gagttcctga tgagacactt
     1681 gtctcttcta gctgactatt gttccatcac aaatatgcat gcaaaaaatc tagcaattgt
     1741 ttgggctcca aacctgttaa gatcaaaaca gatagaatct gcctgcttca gtggaacagc
     1801 agctttcatg gaagtgagga ttcagtctgt ggttgttgag ttcatcctga atcacgttga
     1861 tgtgctgttc agcggcagaa tcagcatggc catgcaagag ggggcagctt ctctatcaag
     1921 gcccaagtcc ctcctggtat cctctccatc caccaaactg ctgacattgg aagaggccca
     1981 ggcacgaaca caagctcagg tcaattctcc aattgtgacg gaaaataaat atatcgaagt
     2041 aggagaagga cctgctgcac ttcaggggaa atttcatacc ataattgagt tcccacttga
     2101 aagaaagagg cctcaaaata agatgaaaaa gtctcctgtg ggtagctggc gttccttttt
     2161 caacttgggg aaatcatcat ctgtttctaa acgaaagctg cagcggaatg agagtgagcc
     2221 ttcagagatg aaagccatgg ctctgaaagg tggcagggca gaaggaaccc tccgttcagc
     2281 taaaagtgag gagtctctta catctctcca tgcagttgat ggtgattcta agctcttccg
     2341 acccagaaga cccagatctt ccagtgatgc actgtctgcc tcttttaatg gagaaatgct
     2401 ggggaaccgc tgtaactcct atgataatct gcctcatgac aatgagagtg aggaggaagg
     2461 agggctgctt catatcccag cccttatgtc tcctcattca gctgaggatg ttgacttgag
     2521 cccaccagac attggagtag ccagcctgga ttttgatcca atgtcatttc aatgtagtcc
     2581 tcctaaggcc gaatcagaat gtctggagag tggtgcttcc tttttagatt caccaggata
     2641 ctccaaggat aaaccaagtg ccaataaaaa ggatgcagaa acaggtagta gccaatgtca
     2701 gactccagga agcacagcaa gctctgaacc tgtctctcct cttcaggaga aactgagtcc
     2761 attctttacc ctggacttga gcccaactga agataaatca tctaagccat cctcctttac
     2821 tgaaaaggtc gtctatgctt tctctccgaa gataggacgg aaattaagca aatcaccttc
     2881 tatgagcata tctgagccaa tttcagtgac cctaccacca cgggtgtcag aagtcattgg
     2941 tacagtctca aataccacag ctcagaatgc atcatcttca acctgggaca aatgcgttga
     3001 agaaagggat gccacaaata gatcccccac ccagatagta aagatgaaaa caaatgagac
     3061 agttgcccaa gaagcatatg aatctgaagt ccagcccctg gaccaggtgg ctgctgaaga
     3121 agtagaattg ccagggaaag aggatcagtc tgtctcaagc agtcagagta aggctgtagc
     3181 ttctggacag actcagacag gagcagttac ccatgacccc cctcaggatt ccgttcctgt
     3241 cagttcagtc tctcttatcc caccaccacc gcctccgaaa aatgttgccc gaatgttggc
     3301 gctagcatta gctgagtccg cacagcaagc ctcaactcag tcattgaaga gaccagggac
     3361 ctctcaggct gggtatacaa attatggaga catagcggtg gctacaactg aagataatct
     3421 gtccagttct tactctgcag ttgctctaga taaggcctat ttccaaaccg atcgaccagc
     3481 agagcagttc cacctccaga ataatgcacc aggaaactgt gaccatcctc taccagagac
     3541 aacagctact ggggatccta cccattccaa cacaactgaa tctggggagc aacatcacca
     3601 agtagactta acagggaatc agccacatca agcatattta tctggggacc cagaaaaggc
     3661 cagaattact tcagttccct tagactcaga gaagtctgat gatcatgtaa gtttccctga
     3721 agaccagtct gggaagaaca gtatgccaac tgtctccttc ttggatcagg accagtctcc
     3781 accccgtttc tacagtggag atcagcctcc ttcttatctt ggtgcaagtg tggataaact
     3841 ccatcaccct ttagaatttg cagacaaatc tcccacacct cctaatttac ctagcgataa
     3901 aatctaccct ccttctgggt cccccgaaga gaataccagc acagccacca tgacttacat
     3961 gacaactact ccagcaacag cccaaatgag caccaaggaa gccagctggg atgtggctga
     4021 acaacccacc actgctgatt ttgctgctgc cacacttcag cgcacgcaca gaactaatcg
     4081 tccccttccc cctccgcctt cccagagatc tgcagagcag ccaccagttg tggggcaggt
     4141 acaagcagca accaatatag gattaaataa ttcccacaag gttcaaggag tagttccagt
     4201 tccagagagg ccacctgaac ctcgagccat ggatgaccct gcgtctgcct tcatcagtga
     4261 cagtggtgct gctgctgctc agtgtcccat ggctacagct gtccagccag gcctgcctga
     4321 gaaagtgcgg gacggtgccc gggtcccgct gctgcacctg cgcgccgagt ctgtccctgc
     4381 gcatccctgt ggctttcctg caccactgcc ccccaccagg atgatggaga gtaagatgat
     4441 tgctgccata cactccagca gtgcagatgc caccagcagt tcaaattatc attcctttgt
     4501 cactgcttca tccacctctg tggacgatgc attgccttta ccacttcctg tcccacaacc
     4561 taagcatgct tctcagaaaa cagtttactc ctcctttgct aggcccgatg tcaccactga
     4621 accctttggt ccagataact gtttgcattt caatatgact ccaaactgcc agtaccgtcc
     4681 ccagagtgta cctccccatc acaataaatt ggagcagcac caagtgtatg gtgccaggtc
     4741 agagccacca gcctccatgg gtcttcgtta taacacatat gtggccccag gaagaaacgc
     4801 atctggacac cactccaagc catgcagccg ggtcgagtat gtgtcttctt tgagctcctc
     4861 tgtcaggaat acctgttacc ccgaagacat tccaccgtac cctaccatcc ggagagtgca
     4921 gtctctccat gctccgccgt cttccatgat tcgctctgtt cccatttcac ggacagaagt
     4981 tcccccagat gatgagccag cctactgccc aagacctctg taccaatata agccatatca
     5041 gtcctcccag gcccgctcag attatcatgt cactcagctt cagccttact ttgagaatgg
     5101 ccgggtccac tacaggtata gcccatattc cagttcttct agttcctatt acagtccaga
     5161 tggggccctg tgtgatgtgg atgcctatgg cacagtccag ttgagacccc ttcaccgcct
     5221 tcccaatcga gactttgctt tctacaatcc taggctgcaa ggaaagagct tgtacagtta
     5281 tgctggtttg gctccacgtc cccgggccaa cgtgactggc tatttctctc ccaacgacca
     5341 taatgtagtc agcatgcctc cggctgctga tgtgaagcac acctacacct catgggatct
     5401 tgaggacatg gaaaaatacc gcatgcagtc catccggaga gagagccgtg ctcggcagaa
     5461 ggtgaaaggg cctgtcatgt cccaatatga taacatgacc ccggcggtgc aggacgactt
     5521 gggtgggatc tatgtcatcc atctgcgtag taaatcagat cctgggaaaa ctggacttct
     5581 ctcagtggca gaaggaaagg agagccgcca tgcagccaag gccatcagtc ccgagggaga
     5641 ggaccgcttc tataggaggc atcccgaggc agagatggac agagcccacc atcacggagg
     5701 ccatggtagc acgcagccgg agaagccatc cctgcctcag aagcagagca gcctgaggag
     5761 caggaagctt cctgacatgg gctgcagtct tcctgagcac agggcacacc aagaagcaag
     5821 ccataggcag ttctgtgagt caaagaatgg gcccccttat ccccagggag ctggccagtt
     5881 agattatggg tccaaaggga ttccagacac ttctgagcca gtcagctacc acaactctgg
     5941 agtaaaatat gctgcatccg ggcaagaatc tttaagactg aaccacaaag aggtaaggct
     6001 ctccaaagag atggagcgac cctgggttag gcagccttct gccccagaga aacactccag
     6061 agactgctac aaggaggaag aacacctcac tcagtcaatc gtcccacccc ctaaaccaga
     6121 gaggagtcat agcctcaaac tccatcatac ccagaacgtg gagagggacc ccagtgtgct
     6181 gtaccagtac caaccacacg gcaagcgcca gagcagtgtg actgttgtgt cccagtatga
     6241 taacctggaa gattaccact ccctgcctca gcaccagcga ggagtctttg gagggggcgg
     6301 catggggacg tatgtgcccc ctggctttcc ccatccacag agcaggacct atgctacagc
     6361 gttgggtcaa ggggccttcc tgcccgcaga gttgtccttg cagcatcctg aaacacagat
     6421 ccatgcagaa tgagccctgc gagcaataga gttgaagcag cctctgctgg acagtggact
     6481 gttctatttt tttcaataac caaaaagatt aaacaaaaaa tactataaaa cccctgacca
     6541 catttaaaaa atgataataa aagtaaacaa atcagcatct ttttcccctt ccctgcttca
     6601 ttaccccctc ttccatctat agactttgtc atttttgtct ttagaaaaga tctgaaggat
     6661 ggtaaagccc cgtgctgaaa cccagtagag aaacctgtct caggacacac ttgccatcta
     6721 gggctagctt gaaagagcct gaggactgcc tttaactgaa tttgaattca gcattgtcct
     6781 ttcttcttag tatttgctgc ataattgaga gcagttcaca tcgatttcct ggtaggcgtc
     6841 tgcattccct gttgtgttcc tgcttctcct tcagtagctg cacaacttgc gcagatcgac
     6901 acactgttgt cacttcattc tccccgtctg agaaggatct tgtgttcagt tagagtcgtg
     6961 gaaaaatccc tgatccttca aggtcagtca gacagttggc aacattataa ttaaaaataa
     7021 gaaattaaga ctttaaatta aacatttggt agagtcatca taaaacacca gaccacttag
     7081 actcaggctg aaccatactc tttctattct tatttttcat ccttgttcct cacggttcag
     7141 tgaacaggct catatcatga cagaatggac ttttaaaagt tagtacttaa ggaaacttct
     7201 ttaggtggaa gaaagtaaag ttcttattgt cagtgaactt tattagcacc agaaatctct
     7261 attgatgctt ttaatgcatt gcctgccttc aggttttctt cttaccccac ccctcaataa
     7321 gatttggtga attgtaattc tagtaaaaca tgtcatacca ttggttttcc taaattatca
     7381 actttctttc attaaaaaaa aaaaaaaaaa aaaaagccca gcatggtttg actggataga
     7441 cacgcataat ttattatgaa tataaatttc catgtttgtt tctgttccta aaccagagta
     7501 cgaggtccct gggaatttaa gtagctacgc attatctatt attagactgc aagttcctgc
     7561 aataactgct tagttcacag ccccgtttca ccagtggagt tctgggcagt tattgctgtc
     7621 ctaaggcatt actgtcgttt gcttactcta tacttgtgtg gtcacagtct tcttgtaatt
     7681 accatctaca ccagcatttc aggtatagct ctttataact ctggagacat gtaaaacatg
     7741 tttaacaccc acgagttttg aaagttgcat tccttattag agtaggaact ctctagccca
     7801 actccattct atgttctcag ctccctccac cccccaaaat acatcagact agcaaggcag
     7861 tcctatgttt acaaaacgag tttagattgt catttcattc cataactctt aataatactc
     7921 aagttttata cattcacgta ttttaaatgc tcggtctgta gaagacacta ggagaattgc
     7981 attccaatta ctggatggtt gctgctctgg ctttttagaa cttgaaatta atttttattt
     8041 agagcaaagg aggaaatctt ttaagaggct aaaatcatgc tgctattatt gctgtgaaat
     8101 tgtataaaga ttaggattca tgccagtttt tattttaaaa aataatgtgc attttaaggg
     8161 tttatattta gaaaaaaata aaatgtttca agaacaacac attgatatgt ggaaatattc
     8221 tataaggttt tcttttgttc ccttagaatt cattggaggg atgcagtaaa aactgtagta
     8281 gaaaccttga aacacccata tgtgaaaagg tctgtggaaa ttgaggcctc tacattaaaa
     8341 gtgcagaacc aactgtttta cagtcaaagt gctaggaaac ctgataggat acttcccttt
     8401 ggcacaaaaa caccctgggt gctacataca ggagtatgac ctttggtgaa tatgtggcac
     8461 taattttttt taccttaatc atattcttgt caagtaggca acccattgcc ccttggagac
     8521 cacaccagcc ctgtaagttc tcaccagcag catggagatt aggaagaggg gctgctgtga
     8581 ccaggagata cacacggctt taagtaactg agagcctaaa gaaagtaacc cagggagtcc
     8641 ggtccagttt taatatttgt ggatttgttg tcacacacat tgtttagtcc tgaaactaaa
     8701 acctatttta taaatagtag ggttaattcc tcgaaacaat ttctttatta ataaatgtcc
     8761 tgtgggttta gaaatatcag gtaaatattt gaatacagaa tgatgattgc aattactgtt
     8821 acaagcgtga aacacaaact tcagatcaaa tctagagttg cttcatttaa tgcatgctag
     8881 caacagcctt aactttggat tcagttattt gaaacacttt tccggcatct ttccctttct
     8941 aatgttgtgg ggtggaaacc ggatggcaaa tcactgtgag ccggatacct cagcacagtc
     9001 caccttgtgt gtgacttcac aaatggggga cttcacaaat ggggtaactg aatgttatta
     9061 ctttcaaatt ttgacatgga gcattatgat caaggaaatg gagctgcctt atacattaaa
     9121 cccgtgattt aatcctattg acattttcat agccatgcct ccagatttta tctttttggc
     9181 aaaattctga ttccacagtt tggtctgatt gaaataaata ttccctggac gtctggctaa
     9241 aaattttgct aacaatccca gaggtgccat tttcttatta ataaatttca ttggagcctt
     9301 atttcttact atattcaatt tcgtttcaaa cctgcaagtc cctgggatgg tcccacgact
     9361 agggcctgca catttcttac aatggcaaag cattttttaa aatttagggt caggttgaaa
     9421 aattctagga ctaattctgt agagaggagg gactgttaac taacgtgagt ggggacggag
     9481 gagtaggtta ccacatttgg agcagtaata gatgcaaacg atgtaaattt gaaatttgcc
     9541 cctttagtta aagaaggagc ctgcaaagtc catttctctg ttttcagccc tgtcagtcac
     9601 ccatttagga tgttggcaaa gtactgcttg agcagaatgt gtaagaaagt aataatgaaa
     9661 gcaaaagtat gtcagacagt tacttcttcc acatggttag aggcatgtga ttttcagcac
     9721 tgtgtgttac agaaatgtca ggaatggtgt attataacgt gtgcaagata atgtcagtgt
     9781 gcacagaggg tcttttttcc ttatctgatt agtactgtta atgttcaaag aataaaaatg
     9841 gttttacagt ttagattctg agatagcaaa acctgatttt tcaaccatga cctgcatgag
     9901 agaagcatcc taggaagtct tagatcatac ttttgagttt ttaattttaa tttatatagt
     9961 gtttttttat gtcttaatat ttttgtgaac tggtgtaaat tgttaatgca tataagcttg
    10021 tgtatttttg taaatagttt tgtgatttat ttcttgcccc atatgtaaat atttagagtc
    10081 tcatttcttg caaacttatt tgaagctgag ttgtgggttt gggttttgtt tgtttctttg
    10141 gttgcagggt ggggtggggg gtggcagggg agggaggaag ggatttttgt acctggagat
    10201 ggagatatct tgtggtttaa agcaaatgtc ccactgaaag tgattcaaat atcaacagaa
    10261 ttatttcagg ttaaaacaga
//
