LOCUS       NM_004815               8952 bp    mRNA    linear   PRI 23-JUL-2024
DEFINITION  Homo sapiens Rho GTPase activating protein 29 (ARHGAP29),
            transcript variant 1, mRNA.
ACCESSION   NM_004815
VERSION     NM_004815.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Kansy,M., Wert,K., Kolb,K., Gallwas,J. and Grundker,C.
  TITLE     ARHGAP29 Is Involved in Increased Invasiveness of
            Tamoxifen-resistant Breast Cancer Cells and its Expression Levels
            Correlate With Clinical Tumor Parameters of Breast Cancer Patients
  JOURNAL   Cancer Genomics Proteomics 21 (4), 368-379 (2024)
   PUBMED   38944420
  REMARK    GeneRIF: ARHGAP29 Is Involved in Increased Invasiveness of
            Tamoxifen-resistant Breast Cancer Cells and its Expression Levels
            Correlate With Clinical Tumor Parameters of Breast Cancer Patients.
REFERENCE   2  (bases 1 to 8952)
  AUTHORS   Jiang,X., Du,W., Yang,C., Wang,S., Li,Y., Shen,X., Yang,X., Yao,J.,
            Du,R., Zhang,X., Huang,Y. and Shen,W.
  TITLE     TBX21 attenuates colorectal cancer progression via an
            ARHGAP29/RSK/GSK3beta dependent manner
  JOURNAL   Cell Oncol (Dordr) 46 (5), 1269-1283 (2023)
   PUBMED   37067748
  REMARK    GeneRIF: TBX21 attenuates colorectal cancer progression via an
            ARHGAP29/RSK/GSK3beta dependent manner.
REFERENCE   3  (bases 1 to 8952)
  AUTHORS   Kolb,K., Hellinger,J., Kansy,M., Wegwitz,F., Bauerschmitz,G.,
            Emons,G. and Grundker,C.
  TITLE     Influence of ARHGAP29 on the Invasion of Mesenchymal-Transformed
            Breast Cancer Cells
  JOURNAL   Cells 9 (12), 2616 (2020)
   PUBMED   33291460
  REMARK    GeneRIF: Influence of ARHGAP29 on the Invasion of
            Mesenchymal-Transformed Breast Cancer Cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8952)
  AUTHORS   Shimizu,K., Matsumoto,H., Hirata,H., Ueno,K., Samoto,M., Mori,J.,
            Fujii,N., Kawai,Y., Inoue,R., Yamamoto,Y., Yano,S., Shimabukuro,T.,
            Furutani-Seiki,M. and Matsuyama,H.
  TITLE     ARHGAP29 expression may be a novel prognostic factor of cell
            proliferation and invasion in prostate cancer
  JOURNAL   Oncol Rep 44 (6), 2735-2745 (2020)
   PUBMED   33125156
  REMARK    GeneRIF: ARHGAP29 expression may be a novel prognostic factor of
            cell proliferation and invasion in prostate cancer.
REFERENCE   5  (bases 1 to 8952)
  AUTHORS   Tang,J.X., Xiao,X.S., Wang,K., Jin,J.Y., Fan,L.L. and Xiang,R.
  TITLE     Identification of a Novel Variant of ARHGAP29 in a Chinese Family
            with Nonsyndromic Cleft Lip and Palate
  JOURNAL   Biomed Res Int 2020, 8790531 (2020)
   PUBMED   33150183
  REMARK    GeneRIF: Identification of a Novel Variant of ARHGAP29 in a Chinese
            Family with Nonsyndromic Cleft Lip and Palate.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8952)
  AUTHORS   Leslie,E.J., Mansilla,M.A., Biggs,L.C., Schuette,K., Bullard,S.,
            Cooper,M., Dunnwald,M., Lidral,A.C., Marazita,M.L., Beaty,T.H. and
            Murray,J.C.
  TITLE     Expression and mutation analyses implicate ARHGAP29 as the
            etiologic gene for the cleft lip with or without cleft palate locus
            identified by genome-wide association on chromosome 1p22
  JOURNAL   Birth Defects Res A Clin Mol Teratol 94 (11), 934-942 (2012)
   PUBMED   23008150
  REMARK    GeneRIF: ARHGAP29 is the etiologic gene for the cleft lip with or
            without cleft palate locus identified by genome-wide association on
            chromosome 1p22.
REFERENCE   7  (bases 1 to 8952)
  AUTHORS   Takefuji,M., Asano,H., Mori,K., Amano,M., Kato,K., Watanabe,T.,
            Morita,Y., Katsumi,A., Itoh,T., Takenawa,T., Hirashiki,A.,
            Izawa,H., Nagata,K., Hirayama,H., Takatsu,F., Naoe,T., Yokota,M.
            and Kaibuchi,K.
  TITLE     Mutation of ARHGAP9 in patients with coronary spastic angina
  JOURNAL   J Hum Genet 55 (1), 42-49 (2010)
   PUBMED   19911011
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 8952)
  AUTHORS   Ripperger,T., von Neuhoff,N., Kamphues,K., Emura,M., Lehmann,U.,
            Tauscher,M., Schraders,M., Groenen,P., Skawran,B., Rudolph,C.,
            Callet-Bauchu,E., van Krieken,J.H., Schlegelberger,B. and
            Steinemann,D.
  TITLE     Promoter methylation of PARG1, a novel candidate tumor suppressor
            gene in mantle-cell lymphomas
  JOURNAL   Haematologica 92 (4), 460-468 (2007)
   PUBMED   17488656
  REMARK    GeneRIF: PARG1 expression was substantially reduced & it displayed
            at least partial promoter methylation in all investigated
            mantle-cell lines & in 31 primary cases. PARG1 is a strong
            candidate tumor suppressor gene in MCL.
REFERENCE   9  (bases 1 to 8952)
  AUTHORS   Myagmar,B.E., Umikawa,M., Asato,T., Taira,K., Oshiro,M., Hino,A.,
            Takei,K., Uezato,H. and Kariya,K.
  TITLE     PARG1, a protein-tyrosine phosphatase-associated RhoGAP, as a
            putative Rap2 effector
  JOURNAL   Biochem Biophys Res Commun 329 (3), 1046-1052 (2005)
   PUBMED   15752761
REFERENCE   10 (bases 1 to 8952)
  AUTHORS   Saras,J., Franzen,P., Aspenstrom,P., Hellman,U., Gonez,L.J. and
            Heldin,C.H.
  TITLE     A novel GTPase-activating protein for Rho interacts with a PDZ
            domain of the protein-tyrosine phosphatase PTPL1
  JOURNAL   J Biol Chem 272 (39), 24333-24338 (1997)
   PUBMED   9305890
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL162735.41, BC093767.1 and
            BF115799.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_004815.3.
            
            Summary: Rap1 is a small GTPase that, through effectors, regulates
            Rho GTPase signaling. These effectors- Rasip1, Radil, and the
            protein encoded by this gene- translocate to the cell membrane,
            where they form a multiprotein complex. This complex is necessary
            for Rap1-induced inhibition of Rho signaling. Defects in this gene
            may be a cause of nonsyndromic cleft lip with or without cleft
            palate. [provided by RefSeq, Jun 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a). Variants 1 and 2
            both encode the same isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.262007.1,
                                           SRR1660803.16576.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000260526.11/ ENSP00000260526.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-157               AL162735.41        77049-77205         c
            158-3965            BC093767.1         1-3808
            3966-8522           AL162735.41        8956-13512          c
            8523-8950           BF115799.1         5-432               c
            8951-8952           AL162735.41        8526-8527           c
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p22.1-p21.3"
     gene            1..8952
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /note="Rho GTPase activating protein 29"
                     /db_xref="GeneID:9411"
                     /db_xref="HGNC:HGNC:30207"
                     /db_xref="MIM:610496"
     exon            1..170
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            171..407
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     CDS             203..3988
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /note="isoform a is encoded by transcript variant 1;
                     PTPL1-associated RhoGAP 1 (PARG1); PTPL1-associated RhoGAP
                     protein 1; rho-type GTPase-activating protein 29"
                     /codon_start=1
                     /product="rho GTPase-activating protein 29 isoform a"
                     /protein_id="NP_004806.3"
                     /db_xref="CCDS:CCDS748.1"
                     /db_xref="GeneID:9411"
                     /db_xref="HGNC:HGNC:30207"
                     /db_xref="MIM:610496"
                     /translation="MIAHKQKKTKKKRAWASGQLSTDITTSEMGLKSLSSNSIFDPDY
                     IKELVNDIRKFSHMLLYLKEAIFSDCFKEVIHIRLEELLRVLKSIMNKHQNLNSVDLQ
                     NAAEMLTAKVKAVNFTEVNEENKNDLFQEVFSSIETLAFTFGNILTNFLMGDVGNDSL
                     LRLPVSRETKSFENVSVESVDSSSEKGNFSPLELDNVLLKNTDSIELALSYAKTWSKY
                     TKNIVSWVEKKLNLELESTRNMVKLAEATRTNIGIQEFMPLQSLFTNALLNDIESSHL
                     LQQTIAALQANKFVQPLLGRKNEMEKQRKEIKELWKQEQNKMLEAENALKKAKLLCMQ
                     RQDEYEKAKSSMFRAEEEHLSSSGGLAKNLNKQLEKKRRLEEEALQKVEEANELYKVC
                     VTNVEERRNDLENTKREILAQLRTLVFQCDLTLKAVTVNLFHMQHLQAASLADSLQSL
                     CDSAKLYDPGQEYSEFVKATNSTEEEKVDGNVNKHLNSSQPSGFGPANSLEDVVRLPD
                     SSNKIEEDRCSNSADITGPSFIRSWTFGMFSDSESTGGSSESRSLDSESISPGDFHRK
                     LPRTPSSGTMSSADDLDEREPPSPSETGPNSLGTFKKTLMSKAALTHKFRKLRSPTKC
                     RDCEGIVVFQGVECEECLLVCHRKCLENLVIICGHQKLPGKIHLFGAEFTQVAKKEPD
                     GIPFILKICASEIENRALCLQGIYRVCGNKIKTEKLCQALENGMHLVDISEFSSHDIC
                     DVLKLYLRQLPEPFILFRLYKEFIDLAKEIQHVNEEQETKKNSLEDKKWPNMCIEINR
                     ILLKSKDLLRQLPASNFNSLHFLIVHLKRVVDHAEENKMNSKNLGVIFGPSLIRPRPT
                     TAPITISSLAEYSNQARLVEFLITYSQKIFDGSLQPQDVMCSIGVVDQGCFPKPLLSP
                     EERDIERSMKSLFFSSKEDIHTSESESKIFERATSFEESERKQNALGKCDACLSDKAQ
                     LLLDQEAESASQKIEDGKTPKPLSLKSDRSTNNVERHTPRTKIRPVSLPVDRLLLASP
                     PNERNGRNMGNVNLDKFCKNPAFEGVNRKDAATTVCSKFNGFDQQTLQKIQDKQYEQN
                     SLTAKTTMIMPSALQEKGVTTSLQISGDHSINATQPSKPYAEPVRSVREASERRSSDS
                     YPLAPVRAPRTLQPQHWTTFYKPHAPIISIRGNEEKPASPSAAVPPGTDHDPHGLVVK
                     SMPDPDKASACPGQATGQPKEDSEELGLPDVNPMCQRPRLKRMQQFEDLEGEIPQFV"
     exon            408..542
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            543..639
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            640..712
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            713..761
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            762..899
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            900..964
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1075
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1156
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1345
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1483
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1641
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1642..1778
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1883
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..1982
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2122
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2123..2311
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2312..2449
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2450..2682
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2683..2998
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2999..3107
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     exon            3108..8952
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5224..5229
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /note="hexamer: AATATA"
     polyA_site      5259
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /note="major polyA site"
     regulatory      6068..6073
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /note="hexamer: AATAAA"
     polyA_site      6106
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
     regulatory      7093..7098
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /note="hexamer: AATAAA"
     polyA_site      7123
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
     polyA_site      7206
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
     regulatory      8931..8936
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
                     /note="hexamer: ATTAAA"
     polyA_site      8952
                     /gene="ARHGAP29"
                     /gene_synonym="PARG1"
ORIGIN      
        1 agtggtggtg gtgcctgtgg ctgtggctgc ggctgcggct gcggctgaga tttggccggg
       61 cgtccgcagg ccgtggggga tgggggcagc gagctccagc cctcggcggt ggcggcggcc
      121 gtaggtgtgg ggcgggcgtc cgcgtccggc acgcgagatg gagcgccgtg gatttcagtt
      181 tttctgactg ttacatgaaa ggatgattgc tcacaaacag aaaaagacaa agaaaaaacg
      241 tgcttgggca tcaggtcaac tctctactga tattacaact tctgaaatgg ggctcaagtc
      301 cttaagttcc aactctattt ttgatccgga ttacatcaag gagttggtga atgatatcag
      361 gaagttctcc cacatgttac tatatttgaa agaagccata ttttcagact gttttaaaga
      421 agttattcat atacgtctag aggaactgct ccgtgtttta aagtctataa tgaataaaca
      481 tcagaacctc aattctgttg atcttcaaaa tgctgcagaa atgctcactg caaaagtgaa
      541 agctgtgaac ttcacagaag ttaatgaaga aaacaaaaac gatctcttcc aggaagtgtt
      601 ttcttctatt gaaactttgg catttacctt tggaaatatc cttacaaact tccttatggg
      661 agatgtaggc aatgattcat tattgcgact gcctgtttct cgagaaacta agtcgtttga
      721 aaatgtttct gtggaatcag tggactcatc cagtgaaaaa ggaaattttt cccctttaga
      781 actagacaac gtgctgttaa agaacactga ctctatcgag ctggctttgt catatgctaa
      841 aacttggtca aaatatacta agaacatagt ttcatgggtt gaaaaaaagc ttaacttgga
      901 attggagtcc actagaaata tggtcaagtt ggcagaggca actagaacta acattggaat
      961 tcaggagttc atgccactgc agtctctgtt tactaatgct cttcttaatg atatagaaag
     1021 cagtcacctt ttacaacaaa caattgcagc tctccaggct aacaaatttg tgcagcctct
     1081 acttggaagg aaaaatgaaa tggaaaaaca aaggaaagaa ataaaagagc tttggaaaca
     1141 ggagcaaaat aaaatgcttg aagcagagaa tgctctcaaa aaggcaaaat tattatgcat
     1201 gcaacgtcaa gatgaatatg agaaagcaaa gtcttccatg tttcgtgcag aagaggagca
     1261 tctgtcttca agtggcggat tagcaaaaaa tctcaacaag caactagaaa aaaagcgaag
     1321 gttggaagag gaggctctcc aaaaagtaga agaagcaaat gaactttaca aagtttgtgt
     1381 gacaaatgtt gaagaaagaa gaaatgatct agaaaatacc aaaagagaaa ttttagcaca
     1441 actccggaca cttgttttcc agtgtgatct tacccttaaa gctgtaacag ttaacctctt
     1501 ccacatgcag catctgcagg ctgcttccct tgcagacagt ttacagtctc tctgtgatag
     1561 tgccaaactc tatgacccag gccaagagta cagtgaattt gtcaaggcca caaattcaac
     1621 tgaagaagaa aaagttgatg gaaatgtaaa taaacattta aatagttccc aaccttcagg
     1681 atttggacct gccaactctt tagaggatgt tgtacgcctt cctgacagtt ctaataaaat
     1741 tgaagaggac agatgctcta acagtgcaga tataacaggt ccttccttta taagatcatg
     1801 gacatttggg atgtttagtg attctgagag cactggaggg agcagcgaat ctagatctct
     1861 ggattcagaa tctataagtc caggagactt tcatcgaaaa cttccacgaa caccatccag
     1921 tggaactatg tcctctgcag atgatctaga tgaaagagag ccaccttccc cttcagaaac
     1981 tggacccaat tcccttggaa catttaagaa aacattgatg tcaaaggcag ctctcacaca
     2041 caagtttcgc aaattgagat cccccacgaa atgtagggat tgtgaaggca ttgtagtgtt
     2101 ccaaggtgtt gaatgtgaag agtgtctcct tgtttgtcat cgaaagtgtt tggaaaattt
     2161 agtcattatt tgtggtcatc agaaacttcc aggaaaaata cacttatttg gagcagaatt
     2221 cacacaagtt gcaaaaaagg aaccagatgg tatccctttt atactcaaaa tatgtgcctc
     2281 agagattgaa aatagagctt tgtgtctaca gggaatttat cgtgtgtgtg gaaacaaaat
     2341 aaaaactgaa aaattgtgtc aagctttgga aaatggaatg cacttggtag atatttcaga
     2401 atttagttca catgatatct gtgacgtctt gaaattatac cttcggcagc tcccagaacc
     2461 atttatttta tttcgattgt acaaggaatt tatagacctt gcaaaagaga tccaacatgt
     2521 aaatgaagaa caagagacaa aaaagaatag tcttgaagac aaaaaatggc caaatatgtg
     2581 tatagaaata aaccgaattc ttctaaaaag caaagacctt ctaagacaat tgccagcatc
     2641 aaattttaac agtcttcatt tccttatagt acatctaaag cgggtagtag atcatgcaga
     2701 agaaaacaag atgaactcca aaaacttggg ggtgatattt ggaccaagtc tcattaggcc
     2761 aaggcccaca actgctccta tcaccatctc ctcccttgca gagtattcaa atcaagcacg
     2821 cttggtagag tttctcatta cttactcaca gaagatcttc gatgggtccc tacaaccaca
     2881 agatgttatg tgtagcatag gtgttgttga tcaaggctgt tttccaaagc ctctgttatc
     2941 accagaagaa agagacattg aacgttccat gaagtcacta tttttttctt caaaggaaga
     3001 tatccatact tcagagagtg aaagcaaaat ttttgaacga gctacatcat ttgaggaatc
     3061 agaacgcaag caaaatgcgt taggaaaatg tgatgcatgt ctcagtgaca aagcacagtt
     3121 gcttctagac caagaggctg aatcagcatc ccaaaagata gaagatggta aaacccctaa
     3181 gccactttct ctgaaatctg ataggtcaac aaacaatgtg gagaggcata ctccaaggac
     3241 caagattaga cctgtaagtt tgcctgtaga tagactactt cttgcaagtc ctcctaatga
     3301 gagaaatggc agaaatatgg gaaatgtaaa tttagacaag ttttgcaaga atcctgcctt
     3361 tgaaggagtt aatagaaaag acgctgctac tactgtttgt tccaaattta atggctttga
     3421 ccagcaaact ctacagaaaa ttcaggacaa acagtatgaa caaaacagcc taactgccaa
     3481 gactacaatg atcatgccca gtgcactcca ggaaaaagga gtgacaacaa gcctccagat
     3541 tagtggggac cattctatca atgccactca acccagtaag ccatatgcag agccagtcag
     3601 gtcagtgaga gaggcatctg agagacggtc ttcagattcc taccctctcg ctcctgtcag
     3661 agcacccaga acactgcagc ctcaacattg gacaacattt tataaaccac atgctcccat
     3721 catcagtatc agggggaatg aggagaagcc agcttcaccc tcagcagcag tgcctcctgg
     3781 cacagatcac gatccccacg gtctcgtggt gaagtcaatg ccagacccag acaaagcatc
     3841 agcttgtcct gggcaagcaa ctggtcaacc taaagaagac tctgaggagc ttggcttgcc
     3901 tgatgtgaat ccaatgtgtc agagaccaag gctaaaacga atgcaacagt ttgaagacct
     3961 cgaaggtgaa attccacaat ttgtgtaggg atgtcaaatt tcagggtttt tttgttgttg
     4021 ttgtgttatt ttgtggtatt gtgcttgttt tgtgaaagaa tgttttgaca gggcctcttt
     4081 tgtataggac tgccaaatca tgggttttgc cttttgttgt tgtatttatc ctctgttggt
     4141 aatactgaat ggtagaatgt tttgataggg tcacatttgt gcctcactgg aattatcttt
     4201 aaattctgta tttttaaagt tgtgaataag ataggtggat tcgtattttt taaagttcag
     4261 ttgactttcc ccaccaaatg gtccatttga atgcatccct aatatatgat atagtctcaa
     4321 ctaataggtg caatttggga aaatcaggtt tattttttgg agtggaactg ttataagtgc
     4381 ttatttataa aaggaatgtt tctgaatgca agtgcctaaa aagatctttg ttggtatgca
     4441 tatgttttgt cacacaattt tatagtgcat ctttcaccat ttgtgctttt ttaagatacg
     4501 tatgtaagct cttatttttc aattggcaat tcagttaatt tttaaatgtt tacataatgg
     4561 ccagaaggct tgcaaatctg tatttaattg cattttaatt aattgccagt ttttacatgt
     4621 gatagtcagt tgtacaaaga aaatgcactt aaacctgttt ctaaattata tattcagtta
     4681 tattatattt ggctttagat ggttttaata catttgatag tttttcaccc cttggcttta
     4741 ttttatataa acttttgttt ttcagcagtt ctgaactttt tagtatttta taaatggtcc
     4801 aaaaaatgcc tgtttcagaa gtttttgaat tcagtgcatt tcctcttgat ttgtctgggt
     4861 taaaaccatt ccttttgtat gaaatgtttt gacttaggaa tcattttatg tacttgttct
     4921 acctggattg tcaacaactg aaagtacata tttcatccaa atcaagctaa aatttattta
     4981 agttgattct gagagtacag gtcagtaagc ctcattattt ggaatttgag agaaggtata
     5041 ggtgatcgga tctgtttcat ttataaaagg tccagttttt aggactagta cattcctgtt
     5101 attttctggg ttttatcatt ttgcctaaaa taggatataa aagggacaaa aaataagtag
     5161 actgttttta tgtgtgaatt atatttctac taaatgtttt tgtatgactg tgttatactt
     5221 gataatatat atatatatat atatcaactt gttaaattat ttcatgttcc cgtggcttct
     5281 tttcagttgt tgcctattac agtatgagag tttaaggtta ttaaccattg gcttagaagt
     5341 caacacctag gatcatacat ccgttgacta caatgtggaa tgaattttat ggaaacctgt
     5401 tttatagttc tatgtgatgt aaaggtctag ggagcaataa ccagcccttt tttttctgaa
     5461 ggcttgtttt tagtcctcat tgtacaaaat ataaaataat catattgcta tgaataccta
     5521 aaaagaaaat tagagcccat gtgtattgag ccttctctgt gtgccaggaa ctttgccaag
     5581 gtgctacata gattatttta tttcaagttc acagcaacct ataatgggtg ggtaagcatg
     5641 ttattatttc tgtgttacag ataaggaaac taaggcttag ttaaatgact tgcttcagtg
     5701 ttccacaggt gtaacaggtg gagtcaaatc tcaaacatag ggctgtcaga ttttgatggt
     5761 tagtgctctt aatgactgtg attactaaat aatatctggt agtttttaac aatagaaaat
     5821 gcaactttaa aaaattttta ttgtggtaaa atacacataa catttaccat ttttaagtat
     5881 attgtttgaa ccatttttaa gcatatagtt cagtggcatt aagtatattc acattgtttt
     5941 gcaaccatga gcacctattc atctccagaa cattatcatc atcctatact aaaacttaga
     6001 aaatgtactt ttaaacttct tcagttctct tttaatatat ggagcccacc caaaggttat
     6061 atcatgtaat aaactcattt ctctgttttc cctcacagct taaaaatgag aatttcactt
     6121 ttgggtcctt tctgccttta gggctccctg gcactttgtc cagcgtagag ccttaagcct
     6181 ctagatgctt tctagtttcc tggggccctg aggcaagtat ctcttgagaa gaggttgcta
     6241 tttctagagg atccacagtg tgcactgtgt tgtgctatct aatagtgtgg tggaaatcat
     6301 ttatccagaa gttgtttttt gcaacatgga aagatacgtg accaaaagga aagggcaaca
     6361 gcaggagccc tgttagtgct gccagaacac caggaagcct tgtgggaggc gtattgtcca
     6421 agatgatgcg tattgtccaa acgactcaga agaagtcatt tctgaagggt tgatcataac
     6481 ttccctagcc atgttttacc tacagagaac ttagttagaa tttatgagta cagtatgtta
     6541 aattactttt agtgtacctt aggcagtgta tttgttttga tacagagaca aagactatat
     6601 gatccctgag acttgttgcc tagtgaactc acaagcagat agaattgtac ctcagttatt
     6661 tgggtgattt aaagatggta ctatggctac actattccca gctatcgttg tgttacagta
     6721 tttatacaac tggaaacatc tctctaaatg acaaccattg tatattgtta aataacttat
     6781 agtcagtgga attctgtggg tttttctttc ctacaggcag gaaattatgg tgatgtgttg
     6841 atatgattat atatgtagac tattcagtta acatctagga gttagcaaat ttgctacatt
     6901 cacaatcagt atgtaagtca gttgtattcc tagatatcag caactaacag aaatggaagt
     6961 ttttagaaag atctcttcaa tatcattaaa aaatacaaaa tactctacct aagaataagt
     7021 ctaacaaaag atgtacaaga tcttgatgga gaaaatgtta gaccatgtca ggaagcattt
     7081 aaggagatct taaataaatg tagtactcca taatgtacac gaattagaag actcagtcgt
     7141 gtaaagatgt tcatttacat ttgtagattc aatgtaatcc caaccaaaat attttttgca
     7201 ttattacctc tatctactgt gtgtttcctt aagacaaggg cattcctacg taaccatagt
     7261 acaaccatca aacttaggag attgacattg acatcatgat atctaatcca tagaccacat
     7321 tcaatctgct gattttgatt gcctcagtaa tgttcctgtg tttcagcatc ccaatccagg
     7381 accacatagt gcatttagtt gccctatctc ttgtttcatt cagtccagaa catcttttca
     7441 gacttgtctt tgaccttgac acttgaggag gattggccag tttgtagaaa gttcctcagt
     7501 tttggtttat cattagataa gacccataag gtttaggata gacatttttg tccaatacta
     7561 cagaaatgat gctctatact caaatgtgtc atgccaggat gcacacaatg tcaatttgtc
     7621 ttatacctga tagtattaat tttggtcact tggttcagat agtatatacc aggtttctcc
     7681 attgtaaaat tagaattagg aagtaatttg tgaggagatt cttaaagatt gtaaatgtat
     7741 tgtttctcat cgaattgtca cgggctagtc tattgacaat tcttgactga accaattact
     7801 attttggttg tcaaatggtg agtccgtggc ttctgccttg gttggcatcc tactactgta
     7861 aggaagagct cttccttctc tattccttca ttcacttaaa tcagtataga ttcatgaatg
     7921 cctgctttat tcagtgacag tgtattattg tcacttattt tcatgctgaa attttccccg
     7981 atttggtttt tgggagtctc ttcaagctgg cttctgtgtt ccttagagat gcctcattgc
     8041 tttttcccct cttatttttt ttacgagctc atcttacact ttttcctact ccctacccca
     8101 agtctagaat tagccctttt ttccaaggag ccctggtttc tttaaatggg aaatggtata
     8161 tggaaatgaa gacctgagaa aggctcactg tcttattggt gaacagctca aaggaaaacg
     8221 tgtgtctaca cacattcaag aatagtcacc tacttctata tctatttata tacatacgta
     8281 ttgaaaactt tgagttcaca ttgacacctt caattcaatc ctttgaggtt aatctagtct
     8341 ccctttccat ctttgtaact tccttctctc atagaaacct gactcccata atcctaaata
     8401 atttgttcat tcttcctccc tccctcaacc tcttgacatg ccagctgctg tctcatcaca
     8461 cccagtccca aatatgccaa ataaatccgc agtcatagca gcgctggtcc tgaccacacc
     8521 aatccctaac atcctcccca gcctggagcc tggaccatgg cagctgaatc tttgacccca
     8581 gtgatgtcag tcctgagcac accattggaa tcctaggctc ctgtcatctg gctggaagag
     8641 agacaaattc acaacctttt gataatgagc aattattgaa tactcactga atttatcaga
     8701 gaaagtttaa aagtgtacga ttatacatat ttaccaggaa tgtttaaaca tagacattta
     8761 tgaaatttat agaaaatatt gaattgtggt tctcactttt tggtcttggc tgtaacaatt
     8821 tttaaatatt aatacttcct acaataactg tatcatgcta tttttaagtg tcctcacact
     8881 caaccaatct actctgcatc cataaaataa gcatgcagtt cagaaaatag attaaacgac
     8941 taattggcta tg
//
