LOCUS       NM_001366230            5858 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens Rho GTPase activating protein 28 (ARHGAP28),
            transcript variant 3, mRNA.
ACCESSION   NM_001366230 XM_005258144
VERSION     NM_001366230.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Wetherill,L., Agrawal,A., Kapoor,M., Bertelsen,S., Bierut,L.J.,
            Brooks,A., Dick,D., Hesselbrock,M., Hesselbrock,V., Koller,D.L.,
            Le,N., Nurnberger,J.I. Jr., Salvatore,J.E., Schuckit,M.,
            Tischfield,J.A., Wang,J.C., Xuei,X., Edenberg,H.J., Porjesz,B.,
            Bucholz,K., Goate,A.M. and Foroud,T.
  TITLE     Association of substance dependence phenotypes in the COGA sample
  JOURNAL   Addict Biol 20 (3), 617-627 (2015)
   PUBMED   24832863
  REMARK    GeneRIF: The results of this study provide evidence that there are
            common variants within this protein that contribute to the risk for
            a general liability to substance dependence
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Yeung,C.Y., Taylor,S.H., Garva,R., Holmes,D.F., Zeef,L.A.,
            Soininen,R., Boot-Handford,R.P. and Kadler,K.E.
  TITLE     Arhgap28 is a RhoGAP that inactivates RhoA and downregulates stress
            fibers
  JOURNAL   PLoS One 9 (9), e107036 (2014)
   PUBMED   25211221
  REMARK    GeneRIF: these data suggest that the regulation of Rho by RhoGAPs,
            including Arhgap28, during the assembly and development of
            mechanically strong tissues is complex and may involve multiple
            RhoGAPs.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5858)
  AUTHORS   Comuzzie,A.G., Cole,S.A., Laston,S.L., Voruganti,V.S., Haack,K.,
            Gibbs,R.A. and Butte,N.F.
  TITLE     Novel genetic loci identified for the pathophysiology of childhood
            obesity in the Hispanic population
  JOURNAL   PLoS One 7 (12), e51954 (2012)
   PUBMED   23251661
REFERENCE   4  (bases 1 to 5858)
  AUTHORS   Nusbaum,C., Zody,M.C., Borowsky,M.L., Kamal,M., Kodira,C.D.,
            Taylor,T.D., Whittaker,C.A., Chang,J.L., Cuomo,C.A., Dewar,K.,
            FitzGerald,M.G., Yang,X., Abouelleil,A., Allen,N.R., Anderson,S.,
            Bloom,T., Bugalter,B., Butler,J., Cook,A., DeCaprio,D., Engels,R.,
            Garber,M., Gnirke,A., Hafez,N., Hall,J.L., Norman,C.H., Itoh,T.,
            Jaffe,D.B., Kuroki,Y., Lehoczky,J., Lui,A., Macdonald,P.,
            Mauceli,E., Mikkelsen,T.S., Naylor,J.W., Nicol,R., Nguyen,C.,
            Noguchi,H., O'Leary,S.B., O'Neill,K., Piqani,B., Smith,C.L.,
            Talamas,J.A., Topham,K., Totoki,Y., Toyoda,A., Wain,H.M.,
            Young,S.K., Zeng,Q., Zimmer,A.R., Fujiyama,A., Hattori,M.,
            Birren,B.W., Sakaki,Y. and Lander,E.S.
  TITLE     DNA sequence and analysis of human chromosome 18
  JOURNAL   Nature 437 (7058), 551-555 (2005)
   PUBMED   16177791
  REMARK    Erratum:[Nature. 2005 Dec 1;438(7068):696. O'Neill, Keith [added]]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP005205.3 and AP005210.2.
            
            On Sep 26, 2018 this sequence version replaced XM_005258144.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.47690.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: lack of evidence for use of
                                                upstream AUG
            MANE Ensembl match               :: ENST00000383472.9/
                                                ENSP00000372964.4
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-228               AP005205.3         93346-93573
            229-431             AP005210.2         61853-62055
            432-649             AP005210.2         74288-74505
            650-742             AP005210.2         88125-88217
            743-832             AP005210.2         96899-96988
            833-917             AP005210.2         105241-105325
            918-1060            AP005210.2         107681-107823
            1061-1226           AP005210.2         110500-110665
            1227-1318           AP005210.2         110775-110866
            1319-1396           AP005210.2         113222-113299
            1397-1559           AP005210.2         119228-119390
            1560-1642           AP005210.2         124248-124330
            1643-1840           AP005210.2         126979-127176
            1841-1954           AP005210.2         127521-127634
            1955-2011           AP005210.2         131926-131982
            2012-2136           AP005210.2         133593-133717
            2137-2201           AP005210.2         146051-146115
            2202-5858           AP005210.2         149151-152807
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.31"
     gene            1..5858
                     /gene="ARHGAP28"
                     /note="Rho GTPase activating protein 28"
                     /db_xref="GeneID:79822"
                     /db_xref="HGNC:HGNC:25509"
                     /db_xref="MIM:610592"
     exon            1..228
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     CDS             107..2296
                     /gene="ARHGAP28"
                     /note="isoform c is encoded by transcript variant 3;
                     rho-type GTPase-activating protein 28"
                     /codon_start=1
                     /product="rho GTPase-activating protein 28 isoform c"
                     /protein_id="NP_001353159.1"
                     /db_xref="CCDS:CCDS92429.1"
                     /db_xref="GeneID:79822"
                     /db_xref="HGNC:HGNC:25509"
                     /db_xref="MIM:610592"
                     /translation="MEVEDSGGVVLTAYHSYARAQPPNAESRCAPRAAASHPLSRKSI
                     PRCRRINRMLSNESLHPPAFSRSNSEASVDSASMEDFWREIESIKDSSMGGQEEPPPA
                     EVTPVDEGELEAEWLQDVGLSTLISGDEEEDGKALLSTLTRTQAAAVQKRYHTYTQTM
                     RKKDKQSIRDVRDIFGVSESPPRDTCGNHTNQLDGTKEERELPRVIKTSGSMPDDASL
                     NSTTLSDASQDKEGSFAVPRSDSVAILETIPVLPVHSNGSPEPGQPVQNAISDDDFLE
                     KNIPPEAEELSFEVSYSEMVTEALKRNKLKKSEIKKEDYVLTKFNVQKTRFGLTEAGD
                     LSAEDMKKIRHLSLIELTAFFDAFGIQLKRNKTEKVKGRDNGIFGVPLTVLLDGDRKK
                     DPGVKVPLVLQKFFEKVEESGLESEGIFRLSGCTAKVKQYREELDAKFNADKFKWDKM
                     CHREAAVMLKAFFRELPTSLFPVEYIPAFISLMERGPHVKVQFQALHLMVMALPDANR
                     DAAQALMTFFNKVIANESKNRMSLWNISTVMAPNLFFSRSKHSDYEELLLANTAAHII
                     RLMLKYQKILWKVPSFLITQVRRMNEATMLLKKQLPSVRKLLRRKTLERETASPKTSK
                     VLQKSPSARRMSDVPEGVIRVHAPLLSKVSMAIQLNNQTKAKDILAKFQYENSHGSSE
                     CIKIQNQRLYEIGGNIGEHCLDPDAYILDVYRINPQAEWVIKPQQSS"
     exon            229..431
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            432..649
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            650..742
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            743..832
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            833..917
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1060
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1226
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..1318
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1396
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1559
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..1642
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1840
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1841..1954
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1955..2011
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            2012..2136
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            2137..2201
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     exon            2202..5858
                     /gene="ARHGAP28"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5836..5841
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGAP28"
                     /note="hexamer: AATAAA"
     polyA_site      5837
                     /gene="ARHGAP28"
     polyA_site      5858
                     /gene="ARHGAP28"
                     /note="major polyA site"
ORIGIN      
        1 gcacctcggg ccgcgccgcc cagctgctga cagcctcccg gcgcgccggt ccatgctggt
       61 cccggtcttt gttctggggc cggcgccgag acatgcgcgg ctgacgatgg aggtggagga
      121 ctcgggcggc gtggtgctga ccgcctacca ctcgtacgcg cgcgcccagc cccccaacgc
      181 cgagtcgcgc tgcgcgcccc gcgccgcagc cagccacccg ctcagcagaa aatccattcc
      241 tcgctgccga agaattaaca ggatgctctc caatgaatcc ctccatcctc ctgccttcag
      301 ccgttccaac tcagaagcct ccgtagacag cgcctccatg gaggatttct ggcgggaaat
      361 agaaagtatt aaagacagca gcatgggagg gcaagaagag ccaccgccag ctgaggtcac
      421 acctgtggat gaaggagaac ttgaagcaga atggctgcaa gatgtgggtt tatcaactct
      481 gatctcaggt gatgaagagg aagatggcaa agccttgctc tccacattga ctcgaaccca
      541 agcagctgcc gtgcaaaaga gataccatac ctatacccaa accatgagga aaaaggataa
      601 gcaatctatc agggatgtca gagacatttt tggagtcagt gaatctcctc ctcgtgatac
      661 ctgtggcaac cacactaatc agctggatgg caccaaggaa gaaagagagc ttccaagagt
      721 tatcaagaca agtggttcca tgccagatga tgcttctctc aacagtacta ccctgtctga
      781 cgcatcccag gataaagaag ggagttttgc ggttcccagg agtgactctg tggctatact
      841 tgagaccatt ccagttctac cagttcattc caatggatca ccggagcctg gacagccagt
      901 tcagaatgcg ataagtgatg atgattttct ggaaaagaac attccaccag aggctgaaga
      961 gctgtcattt gaagtgtctt attcagaaat ggttacggag gctctaaaaa gaaataaact
     1021 taagaaatca gagattaaga aagaagacta tgttttaact aaatttaatg ttcagaaaac
     1081 cagatttggc ttaactgaag caggagatct gtctgctgaa gacatgaaga aaatccgcca
     1141 tctctctctg attgaattga ctgccttttt tgatgccttt ggaattcaac tgaaaaggaa
     1201 caaaacagag aaagtaaaag gacgagacaa tgggattttt ggagttccac ttacagtcct
     1261 cctggacggt gaccgaaaga aagaccctgg agtgaaagtt cccctggtat tacaaaaatt
     1321 ttttgagaaa gttgaggaat caggtctgga atctgaagga atttttcgac tttcaggatg
     1381 tactgctaaa gtcaagcaat accgtgaaga acttgatgcc aagtttaatg ctgataaatt
     1441 taaatgggac aaaatgtgcc atagagaagc tgcagtaatg ttgaaagcgt ttttcagaga
     1501 actacccacc tctctcttcc ctgtggaata tatacctgcc ttcatcagtc taatggaaag
     1561 agggcctcac gtcaaagtac agtttcaagc cttacacctc atggtcatgg cgctgcctga
     1621 tgccaacaga gatgcagctc aggccctcat gacattcttc aataaagtga ttgccaatga
     1681 atcaaaaaac cgaatgagtc tgtggaacat ttctacagtg atggcaccaa accttttctt
     1741 cagtagaagc aaacactctg attatgaaga attactgtta gcaaacactg cggcccacat
     1801 catccgccta atgcttaagt accagaagat tttgtggaag gttccatctt tcttaatcac
     1861 tcaagtaaga agaatgaatg aagccacgat gctattgaag aagcagctcc caagtgtcag
     1921 gaagctgctc aggaggaaga ccctcgagcg ggagactgca agccccaaga cttcaaaggt
     1981 actgcaaaaa tcaccctcgg caagacgaat gtctgacgtg ccggaaggag tcatacgggt
     2041 ccatgctcca cttctctcca aggtgtccat ggccattcaa ctcaacaatc aaaccaaagc
     2101 caaagacata ttggcaaaat ttcaatatga aaacagtcat ggttcatcag aatgtattaa
     2161 gattcagaac caaaggttat atgaaattgg aggaaatata ggagagcatt gcttggatcc
     2221 agatgcttat atattggatg tatatcgtat aaatcctcaa gcagaatggg tgattaaacc
     2281 ccaacaaagt tcttaaaata tcctcgagag agctgctatc atgtattata tgccaaaaag
     2341 atcctacatt ttggtaggga aaaaacaaca ctgtgtttga cgtatttgtt cctacagcat
     2401 tctcagtatt tctggccctc agcagtgggc attgtaagca tgaactatgg aagaggcgcc
     2461 ggttcaccaa ttcaactgaa gctttctcat gacttttttt tttataaaag taaaggaaag
     2521 atgatgtggt ccttccagaa gaaagaccaa tctagaatat ggaactctaa tcacttctag
     2581 tatttcaact tcctagcaga aatgaacttg gccctagacc tagggataag caatgttctt
     2641 tatgtagcca atgctacgaa acaaaagagg tgaaagagac ccttttttat acttaatgta
     2701 catatattga ctttttgagc aagaatgcca gaaatagcct tcatttctac cctgcaaaat
     2761 aatccagatc tgctttctaa aatgaatcag tttctaaagt gaaacatgca atatttatgc
     2821 tctgactgac tcctgaattg gaagaggaag aacttctgtt tacagaaaac tgtattgtta
     2881 tatatgtcag gctgtgtatt gtgactatca gcattctggt gcaaatgaac ttttctccat
     2941 catcgactgt ggaaaattga tacttttaaa gcatattctt ctatgagcac aggtcctcct
     3001 agtgaagctt agtttgacaa agggtgtcat atgctttcct aacctgattt gtagttaaca
     3061 ttcacagagc ctacattttc tcattacggt tatgatgctc agtatctttc caagtgccag
     3121 gcacgggctt ccttttctga tcaaacatac cattttttat atttcacaac tatagacagt
     3181 cacttctgca gtcccaattt aaaaatgcag aactgcttta tccaagaatg ctgaaaaata
     3241 ctgttctatc caggtttcct aaactataaa agcagatttt gcttttgttt gttaatcata
     3301 ggcatggccg agcattgtgg attagcctga ggcttaaaat cagatgcatg tctggtaaga
     3361 tgaccactgt ctcactatca agagcctgca gagccatttt ccagacctgt gattgcccag
     3421 aacacatagt ccccacgttt ctaatttgga gcaaatctaa aaggtgctga gggattggac
     3481 agctctgact ttcctcgaga ctatggatat agtccttctc agattagctg gaaatgggga
     3541 cagagttctc atttatccca gatatataca ttggcttttt tggaatctat agattgagat
     3601 tttgatgaat ttatattggt tttaatgaga cccaaatgac tctctcttaa caattcaagc
     3661 agtagacaaa gttgctcaca aatacagaaa acatcttttt gtctacttcg caagtctcaa
     3721 aacgtgataa tatttaagcc catttgtaga agaaatcttg tatatactat tattacacct
     3781 tgatatctat acaaaacatc acttgtatta aaatatatag ataactttct ggcatttaga
     3841 attttgttcc attcatttaa tttgttcatc tgagaatttt tttttttgcc tgaaaaccca
     3901 ttaaaagaaa acgtgccttc tgtataagtt tatgtttatt cctacatttt attcattttt
     3961 aatgtatggg taattggtgg cacatgactt tacataatga ctttctagac tctgaaaaaa
     4021 atgcttttat catttttgca attcagacaa gggtaaacat tttactttat gtaattgcca
     4081 catcccaaga tgtaaaacgg cagtaattta tgaccacgta tggaagaaat tcttcaaata
     4141 gctaccttac atttctgcgt atatatgaaa actgaatgat atttctcagt gtatttcctt
     4201 gcatttgaat ggttcaattc tgttgtgttt atgtagaaaa ttcacaaatc tgttcattta
     4261 attttttaat ttgagaattt ttatttcttt tgactgaaaa cacattagaa cctatgagta
     4321 aattctgaag gttttaagat ttacttcagc ttgtcaccag tttcaaataa atccctaata
     4381 ggtaacaagt aaaatacaaa ttcttgtcta ctttcatgtg gttttaaatg gcagggactt
     4441 cgctgagtca gtaagtataa tcactctagt ttattcaagg atgtgtggca actttcaact
     4501 tccatacgta tatatgtatg tatggaaggc catgtcaata ctagtatcat tggatataac
     4561 tttgattctt aatcagaggg caaattcatt agagaagaat ttttagtaga tacacagact
     4621 aatatttgtg tggaggttct tttgaccaat tttattccta agaataaaca aacccctaca
     4681 gttaaaatgc aaagatgcct gtcatctaag tattgaaaga atttttgcct tatcaagagt
     4741 tgttttttaa aaatttaata aattcataaa aaagactcat atctttgcaa acaaaagaag
     4801 ggcagatact gggcttctac atgctatcct taagagcttc tccccctcac ccattcctag
     4861 tgctcttggt atacacttgg gaaaataatg tcctccagga gaaagtgtta gagggaacta
     4921 acattttagg aatgcttatt cagaaaaaaa tcctagagtt gattcattgt tttcctccat
     4981 cctgcaaaag aagatccctt tgttaagcag ctcacttgga aatatgattc ttggagtcaa
     5041 tgatctctaa acaaactgga ttatcaacta tttacaacgt ctatacagta taaactacct
     5101 cacttgcctt tcttgggaaa aaaaaaatga atggacttta acaattgttg ttacaaactg
     5161 tgcctgaatc ttattattta aatcacttca gttagctttc agtgtatgtt taataatata
     5221 catttaatga tgaaaaatat tttcagcaaa gctttaaaac cagaaatact gtgtaactgt
     5281 gatctatgtg gtatactctg agagaattct gtgtcctgct cattgtcttg agtttctaac
     5341 catgtgcaga tgcaagcgtt caggagtagg aattaatgtc catcttttct ttcaggcatt
     5401 ttcatctgct gtccacgtat ttcacttgac tgaaagctca tatgagttaa aatgtccctt
     5461 cttcctagcg agcatatttc aactgttctt cataaatgtt tatgtgcctt aaaagacttt
     5521 atgttaaagg attaaatagt ttctctgaca ggcagttttt aactgttttc cacaaataaa
     5581 aataatatgt catgggatta aaatgtttgt tttcaagcat ttttaacagt tttacacact
     5641 tacatacacc ttaattaaaa atttttttct gtcagacatt taccaactat tttctaaaac
     5701 taatttgaca aatcatgaca ctagaaaacg ccaatgtttt atgtctttgc ccatctcaaa
     5761 agctaatatt gattcttctg ttccatcagc tttcattgtt aagtagaata tgtatgttgc
     5821 attttatcca taagaaataa agagaaaaaa actaaaaa
//
