LOCUS       NM_020824               7381 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens Rho GTPase activating protein 21 (ARHGAP21),
            transcript variant 1, mRNA.
ACCESSION   NM_020824
VERSION     NM_020824.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Xie,Z., Liu,L., Fang,J., Zhong,X., Lin,J. and Chen,F.
  TITLE     [ARHGAP21 inhibits epithelial-mesenchymal transition by
            inactivating the WNT signaling pathway in non-small cell lung
            cancer]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 43 (8), 1322-1332 (2023)
   PUBMED   37712268
  REMARK    GeneRIF: [ARHGAP21 inhibits epithelial-mesenchymal transition by
            inactivating the WNT signaling pathway in non-small cell lung
            cancer].
REFERENCE   2  (bases 1 to 7381)
  AUTHORS   Lazarini,M., Assis-Mendonca,G.R., Machado-Neto,J.A.,
            Latuf-Filho,P., Bezerra,S.M., Vieira,K.P. and Saad,S.T.O.
  TITLE     Silencing of ARHGAP21, a Rho GTPase activating protein (RhoGAP),
            reduces the growth of prostate cancer xenografts in NOD/SCID mice
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1870 (4), 119439 (2023)
   PUBMED   36764390
  REMARK    GeneRIF: Silencing of ARHGAP21, a Rho GTPase activating protein
            (RhoGAP), reduces the growth of prostate cancer xenografts in
            NOD/SCID mice.
REFERENCE   3  (bases 1 to 7381)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   4  (bases 1 to 7381)
  AUTHORS   Wang,Z., Wu,S., Wang,G., Yang,Z., Zhang,Y., Zhu,C. and Qin,X.
  TITLE     ARHGAP21 Is Involved in the Carcinogenic Mechanism of
            Cholangiocarcinoma: A Study Based on Bioinformatic Analyses and
            Experimental Validation
  JOURNAL   Medicina (Kaunas) 59 (1), 139 (2023)
   PUBMED   36676763
  REMARK    GeneRIF: ARHGAP21 Is Involved in the Carcinogenic Mechanism of
            Cholangiocarcinoma: A Study Based on Bioinformatic Analyses and
            Experimental Validation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7381)
  AUTHORS   Menetrey,J., Perderiset,M., Cicolari,J., Dubois,T., Elkhatib,N., El
            Khadali,F., Franco,M., Chavrier,P. and Houdusse,A.
  TITLE     Structural basis for ARF1-mediated recruitment of ARHGAP21 to Golgi
            membranes
  JOURNAL   EMBO J 26 (7), 1953-1962 (2007)
   PUBMED   17347647
  REMARK    GeneRIF: The crystal structure of the activated GTP-bound form of
            ARF1 in a complex with the Arf-binding domain (ArfBD) of ARHGAP21
            at 2.1 A resolution, is presented.
REFERENCE   6  (bases 1 to 7381)
  AUTHORS   Benzinger,A., Muster,N., Koch,H.B., Yates,J.R. 3rd and Hermeking,H.
  TITLE     Targeted proteomic analysis of 14-3-3 sigma, a p53 effector
            commonly silenced in cancer
  JOURNAL   Mol Cell Proteomics 4 (6), 785-795 (2005)
   PUBMED   15778465
REFERENCE   7  (bases 1 to 7381)
  AUTHORS   Dubois,T., Paleotti,O., Mironov,A.A., Fraisier,V., Stradal,T.E., De
            Matteis,M.A., Franco,M. and Chavrier,P.
  TITLE     Golgi-localized GAP for Cdc42 functions downstream of ARF1 to
            control Arp2/3 complex and F-actin dynamics
  JOURNAL   Nat Cell Biol 7 (4), 353-364 (2005)
   PUBMED   15793564
  REMARK    GeneRIF: The ARHGAP10 protein is a novel Rho GTPase-activating
            protein (Rho-GAP) that is recruited to Golgi membranes through
            binding to GTP-ARF1.
REFERENCE   8  (bases 1 to 7381)
  AUTHORS   Meek,S.E., Lane,W.S. and Piwnica-Worms,H.
  TITLE     Comprehensive proteomic analysis of interphase and mitotic
            14-3-3-binding proteins
  JOURNAL   J Biol Chem 279 (31), 32046-32054 (2004)
   PUBMED   15161933
REFERENCE   9  (bases 1 to 7381)
  AUTHORS   Basseres,D.S., Tizzei,E.V., Duarte,A.A., Costa,F.F. and Saad,S.T.
  TITLE     ARHGAP10, a novel human gene coding for a potentially cytoskeletal
            Rho-GTPase activating protein
  JOURNAL   Biochem Biophys Res Commun 294 (3), 579-585 (2002)
   PUBMED   12056806
  REMARK    GeneRIF: ARHGAP10 gene is highly expressed in muscle and brain,
            which are highly differentiated tissues. It supports the hypothesis
            that ARHGAP10 is important for cell differentiation
REFERENCE   10 (bases 1 to 7381)
  AUTHORS   Robertson,N.G., Khetarpal,U., Gutierrez-Espeleta,G.A., Bieber,F.R.
            and Morton,C.C.
  TITLE     Isolation of novel and known genes from a human fetal cochlear cDNA
            library using subtractive hybridization and differential screening
  JOURNAL   Genomics 23 (1), 42-50 (1994)
   PUBMED   7829101
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL355979.11, AV709499.1, AF480466.1, AB037845.2, BI491305.1 and
            BQ026288.1.
            
            On Nov 22, 2018 this sequence version replaced NM_020824.3.
            
            Summary: ARHGAP21 functions preferentially as a GTPase-activating
            protein (GAP) for CDC42 (MIM 116952) and regulates the ARP2/3
            complex (MIM 604221) and F-actin dynamics at the Golgi through
            control of CDC42 activity (Dubois et al., 2005 [PubMed
            15793564]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a). Variants 1, 2, and 3 all encode the same isoform (a).
            
            Sequence Note: The 5'-most in-frame translation start codon is
            selected for this RefSeq and is well-conserved among mammalian
            species. An alternative start codon that would reduce the protein
            length by 1 aa is also present. The use of the downstream start
            codon is assumed in the literature, including PMIDs:12056806,
            15793564 and 17347647.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF480466.1, SRR1803615.86871.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000396432.7/ ENSP00000379709.2
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-219               AL355979.11        16268-16486         c
            220-313             AV709499.1         1-94
            314-1622            AF480466.1         42-1350
            1623-6771           AB037845.2         877-6025
            6772-7378           BI491305.1         22-628              c
            7379-7381           BQ026288.1         24-26               c
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p12.1"
     gene            1..7381
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /note="Rho GTPase activating protein 21"
                     /db_xref="GeneID:57584"
                     /db_xref="HGNC:HGNC:23725"
                     /db_xref="MIM:609870"
     exon            1..326
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            327..769
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     CDS             707..6583
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /note="isoform a is encoded by transcript variant 1; rho
                     GTPase-activating protein 21; Rho-GTPase activating
                     protein 10; rho GTPase-activating protein 10; rho-type
                     GTPase-activating protein 21"
                     /codon_start=1
                     /product="rho GTPase-activating protein 21 isoform a"
                     /protein_id="NP_065875.3"
                     /db_xref="CCDS:CCDS7144.2"
                     /db_xref="GeneID:57584"
                     /db_xref="HGNC:HGNC:23725"
                     /db_xref="MIM:609870"
                     /translation="MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFS
                     WPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTI
                     FVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDE
                     DILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPV
                     EISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIV
                     PSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPA
                     GSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTY
                     KEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQS
                     VQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLEN
                     VNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVN
                     SDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSN
                     SLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKS
                     PSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREK
                     PPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPG
                     DSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQL
                     SHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKS
                     SEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYL
                     YKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLA
                     WIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGA
                     KSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFG
                     VRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMA
                     DIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRL
                     IHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTH
                     MPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVS
                     PGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDE
                     LDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLG
                     KESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLST
                     SSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQS
                     VAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRN
                     SEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKE
                     APSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVG
                     NHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKC
                     SAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSS
                     STLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL
                     "
     exon            770..949
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            950..974
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1067
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1146
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1201
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1231
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..3128
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3129..3287
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3288..3390
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3391..3427
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3428..3553
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3554..3838
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3839..3903
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3904..4040
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4041..4183
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4184..4339
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4340..4418
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4419..4492
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4493..4582
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4583..4708
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4709..4750
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4751..4856
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4857..4888
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4889..7381
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7361..7366
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /note="hexamer: AATAAA"
     polyA_site      7381
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /note="major polyA site"
ORIGIN      
        1 gcttctgtca cttggcgttc gcgggcgccg gagctgggcc ccggcccggc ccggcgaggc
       61 gaggggagag tccggcggcc gccgccccgc cccacgccct cctgcggcgg ccgagagccc
      121 ggggccggcc ggggcaggtc gcggcggtgc ctgggagcgg ccggccgggg gcggcggcct
      181 cggagcgcgg cggcggcggc ggcggcggcg gcgggcagcg cgggatcgtc cggctgcggg
      241 gtccggctca cgccgggcgg gcgggcgggg cgcgctgtcc cggagccggc cagccccgtg
      301 cggatccact cgtcccgctt cctctggatc tctctaggag ctgacactcg aaccttcacg
      361 acccattcgg atttttccag gactcaggag tggcactggg aagaagggga ccgctttctg
      421 caattggcct cgacactggc tgccaagaag acctgtcgcc tttgttttta agtctccaga
      481 aatggaagaa gaaggcgaag tcagttgaag tcacgagaaa tcagcgaggc atttgaaggc
      541 gcttcctgaa aacctttatt ccctggcacg ttgtctgttt caacagccct gcccctcctc
      601 ggagcctgct tgtggaattc ttccccttcg ggtgtgtggt ggcattcccc gccacgtcca
      661 atgtggactc caaaggattt gtcccttctt tgtcatttga aatgaaatga tggccacgcg
      721 tcggactggt ctgtctgagg gagatggtga caagctcaag gcctgcgagg tctcaaaaaa
      781 taaagatgga aaagaacaaa gtgaaactgt atcactgtct gaagatgaaa cattctcctg
      841 gccaggtccc aaaacagtta cgttgaaaag aacatctcaa ggctttggtt ttacattaag
      901 acattttatt gtttatcccc cagagtctgc aattcaattt tcatataagg atgaagaaaa
      961 tggaaacaga ggaggaaaac aaagaaaccg cttggaacca atggatacca tatttgttaa
     1021 gcaagttaaa gaaggaggac ctgcttttga agctggatta tgtacaggtg accgaattat
     1081 aaaagtcaat ggagaaagtg ttattggcaa aacctattcc caagtaattg ctttaattca
     1141 aaacagtgat acaacattgg aacttagtgt tatgccaaaa gatgaagaca ttctccaagt
     1201 gctacagttt acaaaggatg tcacagcact ggcatattct caagatgcct acctgaaagg
     1261 caacgaagct tatagcggca atgcccgcaa tatacctgaa cctccaccaa tctgctatcc
     1321 ctggctgcca tctgccccat cagccatggc acagccagtt gaaatatctc ctcctgactc
     1381 atcattgagc aaacagcaaa ccagtacacc agtactgaca caacctggta gggcctatag
     1441 aatggaaata caagtgcctc catcaccaac agatgttgca aaatcaaaca cagcagtgtg
     1501 tgtttgcaat gaaagtgtaa ggactgtcat tgtgccttct gagaaggttg tagatttgtt
     1561 atccaataga aacaaccata caggtccttc acatagaact gaagaagtga ggtatggcgt
     1621 gagtgagcag acctctttaa aaacagtgtc aagaaccaca tcaccaccat tatcaattcc
     1681 caccactcat ctaattcatc agcctgcagg ctccagatca ctggaacctt ctggaatttt
     1741 acttaagtct ggaaattaca gtggacattc tgatggaatc tcaagcagca gatctcaagc
     1801 tgtggaggct ccctctgtat ctgttaatca ctattcgcca aattcccatc agcacataga
     1861 ctggaaaaac tataaaactt acaaagagta tattgataac agacgattgc acataggttg
     1921 tcggacaata caagaaagat tagatagttt aagagcagca tctcaaagca cgacagatta
     1981 taaccaggtc gtccccaacc gcactacttt gcagggacga cgtcgaagca cctctcatga
     2041 tcgagtgccc cagtctgtcc agatacggca acgcagtgtg tcccaagaaa gactggaaga
     2101 ttctgtgcta atgaagtatt gtccaagaag tgcatctcaa ggagcactga cgtctccatc
     2161 tgttagtttt agtaatcata gaactcgttc atgggattat attgagggac aggatgaaac
     2221 cttagaaaat gtcaattctg gaactccaat acctgattcc aatggagaga aaaaacagac
     2281 ttacaagtgg agtgggttta ctgaacagga tgatagacga ggtatttgtg aaagacctag
     2341 gcagcaagaa attcataaat cttttcgagg ttccaatttt actgtggctc caagcgttgt
     2401 taattctgat aacaggcgaa tgagtggtag aggagtggga tctgtgtcgc agtttaaaaa
     2461 aattccacca gatctaaaaa cattgcagtc aaacagaaat tttcagacta cttgtggaat
     2521 gtcactgcct cggggtattt cacaagacag gtcacctctt gtgaaagtcc gaagtaattc
     2581 tctgaaagct ccttccacgc atgtcacaaa accatcattt agccagaaat catttgtttc
     2641 tatcaaagac caaagaccag taaatcactt gcatcagaac agtctgttga atcagcagac
     2701 atgggtaagg actgacagtg cccccgatca gcaagtggag actgggaaat ccccctcttt
     2761 atctggagcc tctgccaagc ctgcccctca gtcgagtgaa aacgctggta cttcagattt
     2821 agaactacct gtcagtcaaa ggaatcaaga tttaagttta caagaggctg aaactgagca
     2881 atcagatact ttagataata aagaagctgt catcctaagg gaaaaacctc catctggacg
     2941 ccagacaccg cagcctttaa ggcatcagtc ttacatcttg gcagtaaatg accaggagac
     3001 cgggtcagac actacctgct ggctgcccaa tgatgcacgt cgagaggtcc acataaaaag
     3061 aatggaggaa agaaaagcct cgagtaccag tccgcctggc gattctttgg cttccatccc
     3121 atttatagat gaaccaacta gccctagcat tgatcatgat attgcacata tccctgcctc
     3181 tgctgttata tcagcctcta cctctcaggt cccctccata gcaacagttc ctccttgcct
     3241 cacaacttca gctccattaa ttcgccgtca gctctcacat gaccacgaat ctgttggccc
     3301 tcctagcctg gatgctcagc ccaactcaaa gacagaaaga tcaaaatcat atgatgaggg
     3361 tctggatgat tacagagaag atgcaaaatt gtcctttaag cacgtatcta gtctgaaggg
     3421 aatcaagatc gcagacagcc aaaagtcatc agaagactct gggtccagaa aagattcttc
     3481 ctcagaggtc ttcagtgatg ctgccaagga agggtggctt catttccgac cccttgtcac
     3541 cgataagggc aagcgagttg gtggaagtat tcggccatgg aaacagatgt atgttgtcct
     3601 tcggggtcat tcactttacc tgtacaaaga taaaagagag cagacgactc cgtctgagga
     3661 agagcagccc atcagtgtta atgcttgctt gatagacatc tcttacagtg agaccaagag
     3721 gaaaaatgtg tttcgactca ccacgtccga ctgtgaatgc ctgtttcagg ctgaagacag
     3781 agatgatatg ctagcttgga tcaagacgat ccaggagagc agcaacctaa acgaagagga
     3841 cactggagtc actaacaggg atctaattag tcgaagaata aaagaataca acaatctgat
     3901 gagcaaagca gaacagttgc caaaaacacc tcgccagagt ctcagcatca ggcaaacttt
     3961 gcttggtgct aaatcagagc caaagactca aagcccacac tctccgaagg aagagtcgga
     4021 aaggaaactt ctcagtaaag atgataccag tcccccaaaa gacaaaggca catggagaaa
     4081 aggcattcca agtatcatga gaaagacatt tgagaaaaag ccaactgcta caggaacttt
     4141 cggcgtccga ctagatgact gcccaccagc tcatactaat cggtatattc cattaatagt
     4201 tgacatatgt tgcaaattag ttgaagaaag aggtcttgaa tatacaggta tttatagagt
     4261 tcctggaaat aatgcagcca tctcaagtat gcaagaagaa ctcaacaagg gaatggctga
     4321 tattgatata caagatgata aatggcgaga tttgaatgtg ataagcagtt tactaaaatc
     4381 cttcttcaga aaactccctg agcctctctt cacaaatgat aaatatgctg attttattga
     4441 agccaatcgt aaagaagatc ctctagatcg tctgaaaaca ttaaaaagac taattcacga
     4501 tttgcctgaa catcattatg aaacacttaa gttcctttca gctcatctga agacagtggc
     4561 agaaaattca gaaaaaaata agatggaacc aagaaaccta gcaatagtgt ttggtcccac
     4621 ccttgttcga acatcagaag acaacatgac ccacatggtc acccacatgc ctgaccagta
     4681 caagattgta gaaacgctca tccagcacca tgactggttt ttcacagaag aaggtgctga
     4741 agagcctctt acaacagtgc aggaggaaag cacagtagac tcccagccag tgccaaacat
     4801 agatcattta ctcaccaaca ttggaaggac aggagtctcc ccaggagatg tatcagattc
     4861 agctactagt gactcaacaa aatctaaggg ttcttgggga tctggaaagg atcagtatag
     4921 cagggaactg cttgtgtcct ccatctttgc agctgctagt cgcaagagga agaagccgaa
     4981 agaaaaagca cagcctagca gctcagaaga tgaactggac aatgtatttt ttaagaaaga
     5041 aaatgtggaa cagtgtcaca atgatactaa agaggagtcc aaaaaagaaa gtgagacact
     5101 gggcagaaaa cagaagatca tcattgccaa agaaaacagc actaggaaag accccagcac
     5161 gacaaaagat gaaaagatat cactaggaaa agagagcacg ccttctgaag aaccctcacc
     5221 accacacaac tcaaaacaca acaagtcacc aactctcagc tgtcgctttg ccatcctgaa
     5281 agagagcccc aggtcacttc tggcacagaa gtcctcccac cttgaagaga caggctctga
     5341 ctctggcact ttgctcagca cgtcttccca ggcctccctg gcaaggtttt ccatgaagaa
     5401 atcaaccagt ccagaaacga aacatagcga gtttttggcc aacgtcagca ccatcacctc
     5461 agattattcc accacatcgt ctgctacata cttgactagc ctggactcca gtcgactgag
     5521 ccctgaggtg caatccgtgg cagagagcaa gggggacgag gcagatgacg agagaagcga
     5581 actcatcagt gaagggcggc ctgtggaaac cgacagcgag agcgagtttc ccgtgttccc
     5641 cacagccttg acttcagaga ggcttttccg aggaaaactg caagaagtga ctaagagcag
     5701 ccggagaaat tctgaaggaa gtgaattaag ttgcaccgag ggaagtttaa catcaagttt
     5761 agatagccgg agacagctct tcagttccca taaactcatc gaatgtgata ctctttccag
     5821 gaaaaaatca gctagattca agtcagatag tggaagtcta ggagatgcca agaatgagaa
     5881 agaagcacct tcgttaacta aagtgtttga tgttatgaaa aaaggaaagt caactgggag
     5941 tttactgaca cccaccagag gcgaatccga aaaacaggaa cccacatgga aaacgaaaat
     6001 agcagatcgg ttaaaactga gacccagagc ccctgcggat gacatgtttg gagtagggaa
     6061 tcacaaagtg aatgccgaga ctgctaaaag gaaaagcatc cggcgcagac atacactagg
     6121 agggcacaga gatgctaccg aaatcagcgt tttgaatttt tggaaagtgc atgagcagag
     6181 cggggagaga gaatctgaac tttcagctgt aaaccggtta aaaccaaaat gctcagccca
     6241 ggacctttcc atctcagact ggctggccag ggaacgccta cgcaccagta cctctgacct
     6301 tagcagagga gaaatcggag atccccagac agagaaccca agcacacgag aaatagccac
     6361 gaccgacaca cctttgtctc ttcattgcaa cacaggcagt tcttccagca ccttggcttc
     6421 aacaaacagg ccccttcttt ccataccacc acagtcacct gaccaaataa acggagaaag
     6481 cttccagaac gtgagcaaaa atgctagttc tgcagcgaat gcccaacctc ataaactgtc
     6541 tgaaacccca ggcagtaaag cagagtttca tccctgtctt taaactgggg gtatgtccac
     6601 tctagcaagt aaaaaaacta ctgttacacg ttccagtaac tctgtcaata ttttcttgta
     6661 tcagaattgt tattatgcag ccttcatttg ggctggtttc atcattttgc actgtgaaat
     6721 agctttacag tgcattacta cagccagaag aacatatata tatatatata tatttaaaaa
     6781 tatatcggat agttgtatac aaatgagcaa ggtatttgtt gcaacttact acatagcata
     6841 tacccaaaat cactgaagaa aatcgctggc atcagtgtgc agcaaatttg ttcttttggt
     6901 ttcatcacta acaaaagtgc ctcatcataa aaatacagtt ggtttttagg gtgccatatt
     6961 gttaaaatta gataacttac ttacattgaa taaacgaatg cgttttattg gtaacagata
     7021 tcattacatt taccagtttt aacacaggtg gatacagaac ttccattctt tagtcattcc
     7081 aggtggatct gagttttata ttcaaacttt taatacagtt tttgagtttt gtgtgacttg
     7141 aatttttaat ctttctgtaa aatacgtaac ttaaatgaac atattaaatg tgtatctttt
     7201 cttcagatac cagatttgat ataatgttgt aacataggtg tgtagatagt ggatcctgga
     7261 tggaactggc ttctttatcg agaagaatat aattctgcat gaggacttaa tgaatccaaa
     7321 cctgtgtcat gcctgtgtgc atacccaatt aaacactgga aataaaaatt gttttggtga
     7381 a
//
