LOCUS       NM_001367451            7090 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens Rho GTPase activating protein 21 (ARHGAP21),
            transcript variant 5, mRNA.
ACCESSION   NM_001367451 XM_017016461
VERSION     NM_001367451.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Xie,Z., Liu,L., Fang,J., Zhong,X., Lin,J. and Chen,F.
  TITLE     [ARHGAP21 inhibits epithelial-mesenchymal transition by
            inactivating the WNT signaling pathway in non-small cell lung
            cancer]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 43 (8), 1322-1332 (2023)
   PUBMED   37712268
  REMARK    GeneRIF: [ARHGAP21 inhibits epithelial-mesenchymal transition by
            inactivating the WNT signaling pathway in non-small cell lung
            cancer].
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Lazarini,M., Assis-Mendonca,G.R., Machado-Neto,J.A.,
            Latuf-Filho,P., Bezerra,S.M., Vieira,K.P. and Saad,S.T.O.
  TITLE     Silencing of ARHGAP21, a Rho GTPase activating protein (RhoGAP),
            reduces the growth of prostate cancer xenografts in NOD/SCID mice
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1870 (4), 119439 (2023)
   PUBMED   36764390
  REMARK    GeneRIF: Silencing of ARHGAP21, a Rho GTPase activating protein
            (RhoGAP), reduces the growth of prostate cancer xenografts in
            NOD/SCID mice.
REFERENCE   3  (bases 1 to 7090)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   4  (bases 1 to 7090)
  AUTHORS   Wang,Z., Wu,S., Wang,G., Yang,Z., Zhang,Y., Zhu,C. and Qin,X.
  TITLE     ARHGAP21 Is Involved in the Carcinogenic Mechanism of
            Cholangiocarcinoma: A Study Based on Bioinformatic Analyses and
            Experimental Validation
  JOURNAL   Medicina (Kaunas) 59 (1), 139 (2023)
   PUBMED   36676763
  REMARK    GeneRIF: ARHGAP21 Is Involved in the Carcinogenic Mechanism of
            Cholangiocarcinoma: A Study Based on Bioinformatic Analyses and
            Experimental Validation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7090)
  AUTHORS   Menetrey,J., Perderiset,M., Cicolari,J., Dubois,T., Elkhatib,N., El
            Khadali,F., Franco,M., Chavrier,P. and Houdusse,A.
  TITLE     Structural basis for ARF1-mediated recruitment of ARHGAP21 to Golgi
            membranes
  JOURNAL   EMBO J 26 (7), 1953-1962 (2007)
   PUBMED   17347647
  REMARK    GeneRIF: The crystal structure of the activated GTP-bound form of
            ARF1 in a complex with the Arf-binding domain (ArfBD) of ARHGAP21
            at 2.1 A resolution, is presented.
REFERENCE   6  (bases 1 to 7090)
  AUTHORS   Benzinger,A., Muster,N., Koch,H.B., Yates,J.R. 3rd and Hermeking,H.
  TITLE     Targeted proteomic analysis of 14-3-3 sigma, a p53 effector
            commonly silenced in cancer
  JOURNAL   Mol Cell Proteomics 4 (6), 785-795 (2005)
   PUBMED   15778465
REFERENCE   7  (bases 1 to 7090)
  AUTHORS   Dubois,T., Paleotti,O., Mironov,A.A., Fraisier,V., Stradal,T.E., De
            Matteis,M.A., Franco,M. and Chavrier,P.
  TITLE     Golgi-localized GAP for Cdc42 functions downstream of ARF1 to
            control Arp2/3 complex and F-actin dynamics
  JOURNAL   Nat Cell Biol 7 (4), 353-364 (2005)
   PUBMED   15793564
  REMARK    GeneRIF: The ARHGAP10 protein is a novel Rho GTPase-activating
            protein (Rho-GAP) that is recruited to Golgi membranes through
            binding to GTP-ARF1.
REFERENCE   8  (bases 1 to 7090)
  AUTHORS   Meek,S.E., Lane,W.S. and Piwnica-Worms,H.
  TITLE     Comprehensive proteomic analysis of interphase and mitotic
            14-3-3-binding proteins
  JOURNAL   J Biol Chem 279 (31), 32046-32054 (2004)
   PUBMED   15161933
REFERENCE   9  (bases 1 to 7090)
  AUTHORS   Basseres,D.S., Tizzei,E.V., Duarte,A.A., Costa,F.F. and Saad,S.T.
  TITLE     ARHGAP10, a novel human gene coding for a potentially cytoskeletal
            Rho-GTPase activating protein
  JOURNAL   Biochem Biophys Res Commun 294 (3), 579-585 (2002)
   PUBMED   12056806
  REMARK    GeneRIF: ARHGAP10 gene is highly expressed in muscle and brain,
            which are highly differentiated tissues. It supports the hypothesis
            that ARHGAP10 is important for cell differentiation
REFERENCE   10 (bases 1 to 7090)
  AUTHORS   Robertson,N.G., Khetarpal,U., Gutierrez-Espeleta,G.A., Bieber,F.R.
            and Morton,C.C.
  TITLE     Isolation of novel and known genes from a human fetal cochlear cDNA
            library using subtractive hybridization and differential screening
  JOURNAL   Genomics 23 (1), 42-50 (1994)
   PUBMED   7829101
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL355979.11 and AL392104.19.
            
            On Nov 30, 2018 this sequence version replaced XM_017016461.1.
            
            Summary: ARHGAP21 functions preferentially as a GTPase-activating
            protein (GAP) for CDC42 (MIM 116952) and regulates the ARP2/3
            complex (MIM 604221) and F-actin dynamics at the Golgi through
            control of CDC42 activity (Dubois et al., 2005 [PubMed
            15793564]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (5), as well as variants 4 and 6,
            encodes isoform b.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.231187.1,
                                           SRR1803612.126703.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                AL355979.11        15756-15820         c
            66-508              AL355979.11        14436-14878         c
            509-688             AL392104.19        127460-127639       c
            689-713             AL392104.19        124227-124251       c
            714-806             AL392104.19        92253-92345         c
            807-885             AL392104.19        90644-90722         c
            886-940             AL392104.19        87238-87292         c
            941-2837            AL392104.19        76715-78611         c
            2838-2996           AL392104.19        64987-65145         c
            2997-3099           AL392104.19        64741-64843         c
            3100-3136           AL392104.19        61554-61590         c
            3137-3262           AL392104.19        59220-59345         c
            3263-3547           AL392104.19        57888-58172         c
            3548-3612           AL392104.19        55187-55251         c
            3613-3749           AL392104.19        54689-54825         c
            3750-3892           AL392104.19        53982-54124         c
            3893-4048           AL392104.19        53130-53285         c
            4049-4127           AL392104.19        52959-53037         c
            4128-4201           AL392104.19        52359-52432         c
            4202-4291           AL392104.19        52192-52281         c
            4292-4417           AL392104.19        49129-49254         c
            4418-4459           AL392104.19        48884-48925         c
            4460-4565           AL392104.19        48467-48572         c
            4566-4597           AL392104.19        46513-46544         c
            4598-7090           AL392104.19        40856-43348         c
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p12.1"
     gene            1..7090
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /note="Rho GTPase activating protein 21"
                     /db_xref="GeneID:57584"
                     /db_xref="HGNC:HGNC:23725"
                     /db_xref="MIM:609870"
     exon            1..65
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            66..508
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     CDS             446..6292
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /note="isoform b is encoded by transcript variant 5; rho
                     GTPase-activating protein 21; Rho-GTPase activating
                     protein 10; rho GTPase-activating protein 10; rho-type
                     GTPase-activating protein 21"
                     /codon_start=1
                     /product="rho GTPase-activating protein 21 isoform b"
                     /protein_id="NP_001354380.1"
                     /db_xref="CCDS:CCDS91222.1"
                     /db_xref="GeneID:57584"
                     /db_xref="HGNC:HGNC:23725"
                     /db_xref="MIM:609870"
                     /translation="MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFS
                     WPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTI
                     FVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDE
                     DILQVAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLS
                     KQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLS
                     NRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGI
                     LLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLH
                     IGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQ
                     ERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDS
                     NGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRG
                     VGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVT
                     KPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKP
                     APQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQP
                     LRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFID
                     EPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPP
                     SLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDS
                     SSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTP
                     SEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSN
                     LNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPH
                     SPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAH
                     TNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWR
                     DLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYE
                     TLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVET
                     LIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATS
                     DSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKEN
                     VEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPS
                     PPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFS
                     MKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEAD
                     DERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTE
                     GSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDV
                     MKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAK
                     RKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDW
                     LARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPL
                     LSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL"
     exon            509..688
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            689..713
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            714..806
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            807..885
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            886..940
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            941..2837
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            2838..2996
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            2997..3099
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3100..3136
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3137..3262
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3263..3547
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3548..3612
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3613..3749
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3750..3892
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3893..4048
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4049..4127
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4128..4201
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4202..4291
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4292..4417
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4418..4459
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4460..4565
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4566..4597
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4598..7090
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7070..7075
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /note="hexamer: AATAAA"
     polyA_site      7090
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /note="major polyA site"
ORIGIN      
        1 cggccagcgt ctccgcttca gctcgccgcc gcgcttcggc cgccgggccc gagcgggcgg
       61 gaagggatct ctctaggagc tgacactcga accttcacga cccattcgga tttttccagg
      121 actcaggagt ggcactggga agaaggggac cgctttctgc aattggcctc gacactggct
      181 gccaagaaga cctgtcgcct ttgtttttaa gtctccagaa atggaagaag aaggcgaagt
      241 cagttgaagt cacgagaaat cagcgaggca tttgaaggcg cttcctgaaa acctttattc
      301 cctggcacgt tgtctgtttc aacagccctg cccctcctcg gagcctgctt gtggaattct
      361 tccccttcgg gtgtgtggtg gcattccccg ccacgtccaa tgtggactcc aaaggatttg
      421 tcccttcttt gtcatttgaa atgaaatgat ggccacgcgt cggactggtc tgtctgaggg
      481 agatggtgac aagctcaagg cctgcgaggt ctcaaaaaat aaagatggaa aagaacaaag
      541 tgaaactgta tcactgtctg aagatgaaac attctcctgg ccaggtccca aaacagttac
      601 gttgaaaaga acatctcaag gctttggttt tacattaaga cattttattg tttatccccc
      661 agagtctgca attcaatttt catataagga tgaagaaaat ggaaacagag gaggaaaaca
      721 aagaaaccgc ttggaaccaa tggataccat atttgttaag caagttaaag aaggaggacc
      781 tgcttttgaa gctggattat gtacaggtga ccgaattata aaagtcaatg gagaaagtgt
      841 tattggcaaa acctattccc aagtaattgc tttaattcaa aacagtgata caacattgga
      901 acttagtgtt atgccaaaag atgaagacat tctccaagtg gcatattctc aagatgccta
      961 cctgaaaggc aacgaagctt atagcggcaa tgcccgcaat atacctgaac ctccaccaat
     1021 ctgctatccc tggctgccat ctgccccatc agccatggca cagccagttg aaatatctcc
     1081 tcctgactca tcattgagca aacagcaaac cagtacacca gtactgacac aacctggtag
     1141 ggcctataga atggaaatac aagtgcctcc atcaccaaca gatgttgcaa aatcaaacac
     1201 agcagtgtgt gtttgcaatg aaagtgtaag gactgtcatt gtgccttctg agaaggttgt
     1261 agatttgtta tccaatagaa acaaccatac aggtccttca catagaactg aagaagtgag
     1321 gtatggcgtg agtgagcaga cctctttaaa aacagtgtca agaaccacat caccaccatt
     1381 atcaattccc accactcatc taattcatca gcctgcaggc tccagatcac tggaaccttc
     1441 tggaatttta cttaagtctg gaaattacag tggacattct gatggaatct caagcagcag
     1501 atctcaagct gtggaggctc cctctgtatc tgttaatcac tattcgccaa attcccatca
     1561 gcacatagac tggaaaaact ataaaactta caaagagtat attgataaca gacgattgca
     1621 cataggttgt cggacaatac aagaaagatt agatagttta agagcagcat ctcaaagcac
     1681 gacagattat aaccaggtcg tccccaaccg cactactttg cagggacgac gtcgaagcac
     1741 ctctcatgat cgagtgcccc agtctgtcca gatacggcaa cgcagtgtgt cccaagaaag
     1801 actggaagat tctgtgctaa tgaagtattg tccaagaagt gcatctcaag gagcactgac
     1861 gtctccatct gttagtttta gtaatcatag aactcgttca tgggattata ttgagggaca
     1921 ggatgaaacc ttagaaaatg tcaattctgg aactccaata cctgattcca atggagagaa
     1981 aaaacagact tacaagtgga gtgggtttac tgaacaggat gatagacgag gtatttgtga
     2041 aagacctagg cagcaagaaa ttcataaatc ttttcgaggt tccaatttta ctgtggctcc
     2101 aagcgttgtt aattctgata acaggcgaat gagtggtaga ggagtgggat ctgtgtcgca
     2161 gtttaaaaaa attccaccag atctaaaaac attgcagtca aacagaaatt ttcagactac
     2221 ttgtggaatg tcactgcctc ggggtatttc acaagacagg tcacctcttg tgaaagtccg
     2281 aagtaattct ctgaaagctc cttccacgca tgtcacaaaa ccatcattta gccagaaatc
     2341 atttgtttct atcaaagacc aaagaccagt aaatcacttg catcagaaca gtctgttgaa
     2401 tcagcagaca tgggtaagga ctgacagtgc ccccgatcag caagtggaga ctgggaaatc
     2461 cccctcttta tctggagcct ctgccaagcc tgcccctcag tcgagtgaaa acgctggtac
     2521 ttcagattta gaactacctg tcagtcaaag gaatcaagat ttaagtttac aagaggctga
     2581 aactgagcaa tcagatactt tagataataa agaagctgtc atcctaaggg aaaaacctcc
     2641 atctggacgc cagacaccgc agcctttaag gcatcagtct tacatcttgg cagtaaatga
     2701 ccaggagacc gggtcagaca ctacctgctg gctgcccaat gatgcacgtc gagaggtcca
     2761 cataaaaaga atggaggaaa gaaaagcctc gagtaccagt ccgcctggcg attctttggc
     2821 ttccatccca tttatagatg aaccaactag ccctagcatt gatcatgata ttgcacatat
     2881 ccctgcctct gctgttatat cagcctctac ctctcaggtc ccctccatag caacagttcc
     2941 tccttgcctc acaacttcag ctccattaat tcgccgtcag ctctcacatg accacgaatc
     3001 tgttggccct cctagcctgg atgctcagcc caactcaaag acagaaagat caaaatcata
     3061 tgatgagggt ctggatgatt acagagaaga tgcaaaattg tcctttaagc acgtatctag
     3121 tctgaaggga atcaagatcg cagacagcca aaagtcatca gaagactctg ggtccagaaa
     3181 agattcttcc tcagaggtct tcagtgatgc tgccaaggaa gggtggcttc atttccgacc
     3241 ccttgtcacc gataagggca agcgagttgg tggaagtatt cggccatgga aacagatgta
     3301 tgttgtcctt cggggtcatt cactttacct gtacaaagat aaaagagagc agacgactcc
     3361 gtctgaggaa gagcagccca tcagtgttaa tgcttgcttg atagacatct cttacagtga
     3421 gaccaagagg aaaaatgtgt ttcgactcac cacgtccgac tgtgaatgcc tgtttcaggc
     3481 tgaagacaga gatgatatgc tagcttggat caagacgatc caggagagca gcaacctaaa
     3541 cgaagaggac actggagtca ctaacaggga tctaattagt cgaagaataa aagaatacaa
     3601 caatctgatg agcaaagcag aacagttgcc aaaaacacct cgccagagtc tcagcatcag
     3661 gcaaactttg cttggtgcta aatcagagcc aaagactcaa agcccacact ctccgaagga
     3721 agagtcggaa aggaaacttc tcagtaaaga tgataccagt cccccaaaag acaaaggcac
     3781 atggagaaaa ggcattccaa gtatcatgag aaagacattt gagaaaaagc caactgctac
     3841 aggaactttc ggcgtccgac tagatgactg cccaccagct catactaatc ggtatattcc
     3901 attaatagtt gacatatgtt gcaaattagt tgaagaaaga ggtcttgaat atacaggtat
     3961 ttatagagtt cctggaaata atgcagccat ctcaagtatg caagaagaac tcaacaaggg
     4021 aatggctgat attgatatac aagatgataa atggcgagat ttgaatgtga taagcagttt
     4081 actaaaatcc ttcttcagaa aactccctga gcctctcttc acaaatgata aatatgctga
     4141 ttttattgaa gccaatcgta aagaagatcc tctagatcgt ctgaaaacat taaaaagact
     4201 aattcacgat ttgcctgaac atcattatga aacacttaag ttcctttcag ctcatctgaa
     4261 gacagtggca gaaaattcag aaaaaaataa gatggaacca agaaacctag caatagtgtt
     4321 tggtcccacc cttgttcgaa catcagaaga caacatgacc cacatggtca cccacatgcc
     4381 tgaccagtac aagattgtag aaacgctcat ccagcaccat gactggtttt tcacagaaga
     4441 aggtgctgaa gagcctctta caacagtgca ggaggaaagc acagtagact cccagccagt
     4501 gccaaacata gatcatttac tcaccaacat tggaaggaca ggagtctccc caggagatgt
     4561 atcagattca gctactagtg actcaacaaa atctaagggt tcttggggat ctggaaagga
     4621 tcagtatagc agggaactgc ttgtgtcctc catctttgca gctgctagtc gcaagaggaa
     4681 gaagccgaaa gaaaaagcac agcctagcag ctcagaagat gaactggaca atgtattttt
     4741 taagaaagaa aatgtggaac agtgtcacaa tgatactaaa gaggagtcca aaaaagaaag
     4801 tgagacactg ggcagaaaac agaagatcat cattgccaaa gaaaacagca ctaggaaaga
     4861 ccccagcacg acaaaagatg aaaagatatc actaggaaaa gagagcacgc cttctgaaga
     4921 accctcacca ccacacaact caaaacacaa caagtcacca actctcagct gtcgctttgc
     4981 catcctgaaa gagagcccca ggtcacttct ggcacagaag tcctcccacc ttgaagagac
     5041 aggctctgac tctggcactt tgctcagcac gtcttcccag gcctccctgg caaggttttc
     5101 catgaagaaa tcaaccagtc cagaaacgaa acatagcgag tttttggcca acgtcagcac
     5161 catcacctca gattattcca ccacatcgtc tgctacatac ttgactagcc tggactccag
     5221 tcgactgagc cctgaggtgc aatccgtggc agagagcaag ggggacgagg cagatgacga
     5281 gagaagcgaa ctcatcagtg aagggcggcc tgtggaaacc gacagcgaga gcgagtttcc
     5341 cgtgttcccc acagccttga cttcagagag gcttttccga ggaaaactgc aagaagtgac
     5401 taagagcagc cggagaaatt ctgaaggaag tgaattaagt tgcaccgagg gaagtttaac
     5461 atcaagttta gatagccgga gacagctctt cagttcccat aaactcatcg aatgtgatac
     5521 tctttccagg aaaaaatcag ctagattcaa gtcagatagt ggaagtctag gagatgccaa
     5581 gaatgagaaa gaagcacctt cgttaactaa agtgtttgat gttatgaaaa aaggaaagtc
     5641 aactgggagt ttactgacac ccaccagagg cgaatccgaa aaacaggaac ccacatggaa
     5701 aacgaaaata gcagatcggt taaaactgag acccagagcc cctgcggatg acatgtttgg
     5761 agtagggaat cacaaagtga atgccgagac tgctaaaagg aaaagcatcc ggcgcagaca
     5821 tacactagga gggcacagag atgctaccga aatcagcgtt ttgaattttt ggaaagtgca
     5881 tgagcagagc ggggagagag aatctgaact ttcagctgta aaccggttaa aaccaaaatg
     5941 ctcagcccag gacctttcca tctcagactg gctggccagg gaacgcctac gcaccagtac
     6001 ctctgacctt agcagaggag aaatcggaga tccccagaca gagaacccaa gcacacgaga
     6061 aatagccacg accgacacac ctttgtctct tcattgcaac acaggcagtt cttccagcac
     6121 cttggcttca acaaacaggc cccttctttc cataccacca cagtcacctg accaaataaa
     6181 cggagaaagc ttccagaacg tgagcaaaaa tgctagttct gcagcgaatg cccaacctca
     6241 taaactgtct gaaaccccag gcagtaaagc agagtttcat ccctgtcttt aaactggggg
     6301 tatgtccact ctagcaagta aaaaaactac tgttacacgt tccagtaact ctgtcaatat
     6361 tttcttgtat cagaattgtt attatgcagc cttcatttgg gctggtttca tcattttgca
     6421 ctgtgaaata gctttacagt gcattactac agccagaaga acatatatat atatatatat
     6481 atttaaaaat atatcggata gttgtataca aatgagcaag gtatttgttg caacttacta
     6541 catagcatat acccaaaatc actgaagaaa atcgctggca tcagtgtgca gcaaatttgt
     6601 tcttttggtt tcatcactaa caaaagtgcc tcatcataaa aatacagttg gtttttaggg
     6661 tgccatattg ttaaaattag ataacttact tacattgaat aaacgaatgc gttttattgg
     6721 taacagatat cattacattt accagtttta acacaggtgg atacagaact tccattcttt
     6781 agtcattcca ggtggatctg agttttatat tcaaactttt aatacagttt ttgagttttg
     6841 tgtgacttga atttttaatc tttctgtaaa atacgtaact taaatgaaca tattaaatgt
     6901 gtatcttttc ttcagatacc agatttgata taatgttgta acataggtgt gtagatagtg
     6961 gatcctggat ggaactggct tctttatcga gaagaatata attctgcatg aggacttaat
     7021 gaatccaaac ctgtgtcatg cctgtgtgca tacccaatta aacactggaa ataaaaattg
     7081 ttttggtgaa
//
