LOCUS       NM_001367447            7351 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens Rho GTPase activating protein 21 (ARHGAP21),
            transcript variant 4, mRNA.
ACCESSION   NM_001367447 XM_005252544
VERSION     NM_001367447.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Xie,Z., Liu,L., Fang,J., Zhong,X., Lin,J. and Chen,F.
  TITLE     [ARHGAP21 inhibits epithelial-mesenchymal transition by
            inactivating the WNT signaling pathway in non-small cell lung
            cancer]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 43 (8), 1322-1332 (2023)
   PUBMED   37712268
  REMARK    GeneRIF: [ARHGAP21 inhibits epithelial-mesenchymal transition by
            inactivating the WNT signaling pathway in non-small cell lung
            cancer].
REFERENCE   2  (bases 1 to 7351)
  AUTHORS   Lazarini,M., Assis-Mendonca,G.R., Machado-Neto,J.A.,
            Latuf-Filho,P., Bezerra,S.M., Vieira,K.P. and Saad,S.T.O.
  TITLE     Silencing of ARHGAP21, a Rho GTPase activating protein (RhoGAP),
            reduces the growth of prostate cancer xenografts in NOD/SCID mice
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1870 (4), 119439 (2023)
   PUBMED   36764390
  REMARK    GeneRIF: Silencing of ARHGAP21, a Rho GTPase activating protein
            (RhoGAP), reduces the growth of prostate cancer xenografts in
            NOD/SCID mice.
REFERENCE   3  (bases 1 to 7351)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   4  (bases 1 to 7351)
  AUTHORS   Wang,Z., Wu,S., Wang,G., Yang,Z., Zhang,Y., Zhu,C. and Qin,X.
  TITLE     ARHGAP21 Is Involved in the Carcinogenic Mechanism of
            Cholangiocarcinoma: A Study Based on Bioinformatic Analyses and
            Experimental Validation
  JOURNAL   Medicina (Kaunas) 59 (1), 139 (2023)
   PUBMED   36676763
  REMARK    GeneRIF: ARHGAP21 Is Involved in the Carcinogenic Mechanism of
            Cholangiocarcinoma: A Study Based on Bioinformatic Analyses and
            Experimental Validation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7351)
  AUTHORS   Menetrey,J., Perderiset,M., Cicolari,J., Dubois,T., Elkhatib,N., El
            Khadali,F., Franco,M., Chavrier,P. and Houdusse,A.
  TITLE     Structural basis for ARF1-mediated recruitment of ARHGAP21 to Golgi
            membranes
  JOURNAL   EMBO J 26 (7), 1953-1962 (2007)
   PUBMED   17347647
  REMARK    GeneRIF: The crystal structure of the activated GTP-bound form of
            ARF1 in a complex with the Arf-binding domain (ArfBD) of ARHGAP21
            at 2.1 A resolution, is presented.
REFERENCE   6  (bases 1 to 7351)
  AUTHORS   Benzinger,A., Muster,N., Koch,H.B., Yates,J.R. 3rd and Hermeking,H.
  TITLE     Targeted proteomic analysis of 14-3-3 sigma, a p53 effector
            commonly silenced in cancer
  JOURNAL   Mol Cell Proteomics 4 (6), 785-795 (2005)
   PUBMED   15778465
REFERENCE   7  (bases 1 to 7351)
  AUTHORS   Dubois,T., Paleotti,O., Mironov,A.A., Fraisier,V., Stradal,T.E., De
            Matteis,M.A., Franco,M. and Chavrier,P.
  TITLE     Golgi-localized GAP for Cdc42 functions downstream of ARF1 to
            control Arp2/3 complex and F-actin dynamics
  JOURNAL   Nat Cell Biol 7 (4), 353-364 (2005)
   PUBMED   15793564
  REMARK    GeneRIF: The ARHGAP10 protein is a novel Rho GTPase-activating
            protein (Rho-GAP) that is recruited to Golgi membranes through
            binding to GTP-ARF1.
REFERENCE   8  (bases 1 to 7351)
  AUTHORS   Meek,S.E., Lane,W.S. and Piwnica-Worms,H.
  TITLE     Comprehensive proteomic analysis of interphase and mitotic
            14-3-3-binding proteins
  JOURNAL   J Biol Chem 279 (31), 32046-32054 (2004)
   PUBMED   15161933
REFERENCE   9  (bases 1 to 7351)
  AUTHORS   Basseres,D.S., Tizzei,E.V., Duarte,A.A., Costa,F.F. and Saad,S.T.
  TITLE     ARHGAP10, a novel human gene coding for a potentially cytoskeletal
            Rho-GTPase activating protein
  JOURNAL   Biochem Biophys Res Commun 294 (3), 579-585 (2002)
   PUBMED   12056806
  REMARK    GeneRIF: ARHGAP10 gene is highly expressed in muscle and brain,
            which are highly differentiated tissues. It supports the hypothesis
            that ARHGAP10 is important for cell differentiation
REFERENCE   10 (bases 1 to 7351)
  AUTHORS   Robertson,N.G., Khetarpal,U., Gutierrez-Espeleta,G.A., Bieber,F.R.
            and Morton,C.C.
  TITLE     Isolation of novel and known genes from a human fetal cochlear cDNA
            library using subtractive hybridization and differential screening
  JOURNAL   Genomics 23 (1), 42-50 (1994)
   PUBMED   7829101
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL355979.11 and AL392104.19.
            
            On Nov 30, 2018 this sequence version replaced XM_005252544.4.
            
            Summary: ARHGAP21 functions preferentially as a GTPase-activating
            protein (GAP) for CDC42 (MIM 116952) and regulates the ARP2/3
            complex (MIM 604221) and F-actin dynamics at the Golgi through
            control of CDC42 activity (Dubois et al., 2005 [PubMed
            15793564]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (4), as well as variants 5 and 6,
            encodes isoform b.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: SRR1803611.167940.1, SRR1803615.7900.1
                                    [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1965299,
                                    SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-326               AL355979.11        16161-16486         c
            327-769             AL355979.11        14436-14878         c
            770-949             AL392104.19        127460-127639       c
            950-974             AL392104.19        124227-124251       c
            975-1067            AL392104.19        92253-92345         c
            1068-1146           AL392104.19        90644-90722         c
            1147-1201           AL392104.19        87238-87292         c
            1202-3098           AL392104.19        76715-78611         c
            3099-3257           AL392104.19        64987-65145         c
            3258-3360           AL392104.19        64741-64843         c
            3361-3397           AL392104.19        61554-61590         c
            3398-3523           AL392104.19        59220-59345         c
            3524-3808           AL392104.19        57888-58172         c
            3809-3873           AL392104.19        55187-55251         c
            3874-4010           AL392104.19        54689-54825         c
            4011-4153           AL392104.19        53982-54124         c
            4154-4309           AL392104.19        53130-53285         c
            4310-4388           AL392104.19        52959-53037         c
            4389-4462           AL392104.19        52359-52432         c
            4463-4552           AL392104.19        52192-52281         c
            4553-4678           AL392104.19        49129-49254         c
            4679-4720           AL392104.19        48884-48925         c
            4721-4826           AL392104.19        48467-48572         c
            4827-4858           AL392104.19        46513-46544         c
            4859-7351           AL392104.19        40856-43348         c
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p12.1"
     gene            1..7351
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /note="Rho GTPase activating protein 21"
                     /db_xref="GeneID:57584"
                     /db_xref="HGNC:HGNC:23725"
                     /db_xref="MIM:609870"
     exon            1..326
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            327..769
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     CDS             707..6553
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /note="isoform b is encoded by transcript variant 4; rho
                     GTPase-activating protein 21; Rho-GTPase activating
                     protein 10; rho GTPase-activating protein 10; rho-type
                     GTPase-activating protein 21"
                     /codon_start=1
                     /product="rho GTPase-activating protein 21 isoform b"
                     /protein_id="NP_001354376.1"
                     /db_xref="CCDS:CCDS91222.1"
                     /db_xref="GeneID:57584"
                     /db_xref="HGNC:HGNC:23725"
                     /db_xref="MIM:609870"
                     /translation="MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFS
                     WPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTI
                     FVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDE
                     DILQVAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLS
                     KQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLS
                     NRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGI
                     LLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLH
                     IGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQ
                     ERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDS
                     NGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRG
                     VGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVT
                     KPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKP
                     APQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQP
                     LRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFID
                     EPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPP
                     SLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDS
                     SSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTP
                     SEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSN
                     LNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPH
                     SPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAH
                     TNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWR
                     DLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYE
                     TLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVET
                     LIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATS
                     DSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKEN
                     VEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPS
                     PPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFS
                     MKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEAD
                     DERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTE
                     GSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDV
                     MKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAK
                     RKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDW
                     LARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPL
                     LSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL"
     exon            770..949
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            950..974
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1067
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1146
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1201
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..3098
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3099..3257
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3258..3360
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3361..3397
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3398..3523
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3524..3808
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3809..3873
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            3874..4010
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4011..4153
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4154..4309
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4310..4388
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4389..4462
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4463..4552
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4553..4678
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4679..4720
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4721..4826
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4827..4858
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     exon            4859..7351
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7331..7336
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /note="hexamer: AATAAA"
     polyA_site      7351
                     /gene="ARHGAP21"
                     /gene_synonym="ARHGAP10"
                     /note="major polyA site"
ORIGIN      
        1 gcttctgtca cttggcgttc gcgggcgccg gagctgggcc ccggcccggc ccggcgaggc
       61 gaggggagag tccggcggcc gccgccccgc cccacgccct cctgcggcgg ccgagagccc
      121 ggggccggcc ggggcaggtc gcggcggtgc ctgggagcgg ccggccgggg gcggcggcct
      181 cggagcgcgg cggcggcggc ggcggcggcg gcgggcagcg cgggatcgtc cggctgcggg
      241 gtccggctca cgccgggcgg gcgggcgggg cgcgctgtcc cggagccggc cagccccgtg
      301 cggatccact cgtcccgctt cctctggatc tctctaggag ctgacactcg aaccttcacg
      361 acccattcgg atttttccag gactcaggag tggcactggg aagaagggga ccgctttctg
      421 caattggcct cgacactggc tgccaagaag acctgtcgcc tttgttttta agtctccaga
      481 aatggaagaa gaaggcgaag tcagttgaag tcacgagaaa tcagcgaggc atttgaaggc
      541 gcttcctgaa aacctttatt ccctggcacg ttgtctgttt caacagccct gcccctcctc
      601 ggagcctgct tgtggaattc ttccccttcg ggtgtgtggt ggcattcccc gccacgtcca
      661 atgtggactc caaaggattt gtcccttctt tgtcatttga aatgaaatga tggccacgcg
      721 tcggactggt ctgtctgagg gagatggtga caagctcaag gcctgcgagg tctcaaaaaa
      781 taaagatgga aaagaacaaa gtgaaactgt atcactgtct gaagatgaaa cattctcctg
      841 gccaggtccc aaaacagtta cgttgaaaag aacatctcaa ggctttggtt ttacattaag
      901 acattttatt gtttatcccc cagagtctgc aattcaattt tcatataagg atgaagaaaa
      961 tggaaacaga ggaggaaaac aaagaaaccg cttggaacca atggatacca tatttgttaa
     1021 gcaagttaaa gaaggaggac ctgcttttga agctggatta tgtacaggtg accgaattat
     1081 aaaagtcaat ggagaaagtg ttattggcaa aacctattcc caagtaattg ctttaattca
     1141 aaacagtgat acaacattgg aacttagtgt tatgccaaaa gatgaagaca ttctccaagt
     1201 ggcatattct caagatgcct acctgaaagg caacgaagct tatagcggca atgcccgcaa
     1261 tatacctgaa cctccaccaa tctgctatcc ctggctgcca tctgccccat cagccatggc
     1321 acagccagtt gaaatatctc ctcctgactc atcattgagc aaacagcaaa ccagtacacc
     1381 agtactgaca caacctggta gggcctatag aatggaaata caagtgcctc catcaccaac
     1441 agatgttgca aaatcaaaca cagcagtgtg tgtttgcaat gaaagtgtaa ggactgtcat
     1501 tgtgccttct gagaaggttg tagatttgtt atccaataga aacaaccata caggtccttc
     1561 acatagaact gaagaagtga ggtatggcgt gagtgagcag acctctttaa aaacagtgtc
     1621 aagaaccaca tcaccaccat tatcaattcc caccactcat ctaattcatc agcctgcagg
     1681 ctccagatca ctggaacctt ctggaatttt acttaagtct ggaaattaca gtggacattc
     1741 tgatggaatc tcaagcagca gatctcaagc tgtggaggct ccctctgtat ctgttaatca
     1801 ctattcgcca aattcccatc agcacataga ctggaaaaac tataaaactt acaaagagta
     1861 tattgataac agacgattgc acataggttg tcggacaata caagaaagat tagatagttt
     1921 aagagcagca tctcaaagca cgacagatta taaccaggtc gtccccaacc gcactacttt
     1981 gcagggacga cgtcgaagca cctctcatga tcgagtgccc cagtctgtcc agatacggca
     2041 acgcagtgtg tcccaagaaa gactggaaga ttctgtgcta atgaagtatt gtccaagaag
     2101 tgcatctcaa ggagcactga cgtctccatc tgttagtttt agtaatcata gaactcgttc
     2161 atgggattat attgagggac aggatgaaac cttagaaaat gtcaattctg gaactccaat
     2221 acctgattcc aatggagaga aaaaacagac ttacaagtgg agtgggttta ctgaacagga
     2281 tgatagacga ggtatttgtg aaagacctag gcagcaagaa attcataaat cttttcgagg
     2341 ttccaatttt actgtggctc caagcgttgt taattctgat aacaggcgaa tgagtggtag
     2401 aggagtggga tctgtgtcgc agtttaaaaa aattccacca gatctaaaaa cattgcagtc
     2461 aaacagaaat tttcagacta cttgtggaat gtcactgcct cggggtattt cacaagacag
     2521 gtcacctctt gtgaaagtcc gaagtaattc tctgaaagct ccttccacgc atgtcacaaa
     2581 accatcattt agccagaaat catttgtttc tatcaaagac caaagaccag taaatcactt
     2641 gcatcagaac agtctgttga atcagcagac atgggtaagg actgacagtg cccccgatca
     2701 gcaagtggag actgggaaat ccccctcttt atctggagcc tctgccaagc ctgcccctca
     2761 gtcgagtgaa aacgctggta cttcagattt agaactacct gtcagtcaaa ggaatcaaga
     2821 tttaagttta caagaggctg aaactgagca atcagatact ttagataata aagaagctgt
     2881 catcctaagg gaaaaacctc catctggacg ccagacaccg cagcctttaa ggcatcagtc
     2941 ttacatcttg gcagtaaatg accaggagac cgggtcagac actacctgct ggctgcccaa
     3001 tgatgcacgt cgagaggtcc acataaaaag aatggaggaa agaaaagcct cgagtaccag
     3061 tccgcctggc gattctttgg cttccatccc atttatagat gaaccaacta gccctagcat
     3121 tgatcatgat attgcacata tccctgcctc tgctgttata tcagcctcta cctctcaggt
     3181 cccctccata gcaacagttc ctccttgcct cacaacttca gctccattaa ttcgccgtca
     3241 gctctcacat gaccacgaat ctgttggccc tcctagcctg gatgctcagc ccaactcaaa
     3301 gacagaaaga tcaaaatcat atgatgaggg tctggatgat tacagagaag atgcaaaatt
     3361 gtcctttaag cacgtatcta gtctgaaggg aatcaagatc gcagacagcc aaaagtcatc
     3421 agaagactct gggtccagaa aagattcttc ctcagaggtc ttcagtgatg ctgccaagga
     3481 agggtggctt catttccgac cccttgtcac cgataagggc aagcgagttg gtggaagtat
     3541 tcggccatgg aaacagatgt atgttgtcct tcggggtcat tcactttacc tgtacaaaga
     3601 taaaagagag cagacgactc cgtctgagga agagcagccc atcagtgtta atgcttgctt
     3661 gatagacatc tcttacagtg agaccaagag gaaaaatgtg tttcgactca ccacgtccga
     3721 ctgtgaatgc ctgtttcagg ctgaagacag agatgatatg ctagcttgga tcaagacgat
     3781 ccaggagagc agcaacctaa acgaagagga cactggagtc actaacaggg atctaattag
     3841 tcgaagaata aaagaataca acaatctgat gagcaaagca gaacagttgc caaaaacacc
     3901 tcgccagagt ctcagcatca ggcaaacttt gcttggtgct aaatcagagc caaagactca
     3961 aagcccacac tctccgaagg aagagtcgga aaggaaactt ctcagtaaag atgataccag
     4021 tcccccaaaa gacaaaggca catggagaaa aggcattcca agtatcatga gaaagacatt
     4081 tgagaaaaag ccaactgcta caggaacttt cggcgtccga ctagatgact gcccaccagc
     4141 tcatactaat cggtatattc cattaatagt tgacatatgt tgcaaattag ttgaagaaag
     4201 aggtcttgaa tatacaggta tttatagagt tcctggaaat aatgcagcca tctcaagtat
     4261 gcaagaagaa ctcaacaagg gaatggctga tattgatata caagatgata aatggcgaga
     4321 tttgaatgtg ataagcagtt tactaaaatc cttcttcaga aaactccctg agcctctctt
     4381 cacaaatgat aaatatgctg attttattga agccaatcgt aaagaagatc ctctagatcg
     4441 tctgaaaaca ttaaaaagac taattcacga tttgcctgaa catcattatg aaacacttaa
     4501 gttcctttca gctcatctga agacagtggc agaaaattca gaaaaaaata agatggaacc
     4561 aagaaaccta gcaatagtgt ttggtcccac ccttgttcga acatcagaag acaacatgac
     4621 ccacatggtc acccacatgc ctgaccagta caagattgta gaaacgctca tccagcacca
     4681 tgactggttt ttcacagaag aaggtgctga agagcctctt acaacagtgc aggaggaaag
     4741 cacagtagac tcccagccag tgccaaacat agatcattta ctcaccaaca ttggaaggac
     4801 aggagtctcc ccaggagatg tatcagattc agctactagt gactcaacaa aatctaaggg
     4861 ttcttgggga tctggaaagg atcagtatag cagggaactg cttgtgtcct ccatctttgc
     4921 agctgctagt cgcaagagga agaagccgaa agaaaaagca cagcctagca gctcagaaga
     4981 tgaactggac aatgtatttt ttaagaaaga aaatgtggaa cagtgtcaca atgatactaa
     5041 agaggagtcc aaaaaagaaa gtgagacact gggcagaaaa cagaagatca tcattgccaa
     5101 agaaaacagc actaggaaag accccagcac gacaaaagat gaaaagatat cactaggaaa
     5161 agagagcacg ccttctgaag aaccctcacc accacacaac tcaaaacaca acaagtcacc
     5221 aactctcagc tgtcgctttg ccatcctgaa agagagcccc aggtcacttc tggcacagaa
     5281 gtcctcccac cttgaagaga caggctctga ctctggcact ttgctcagca cgtcttccca
     5341 ggcctccctg gcaaggtttt ccatgaagaa atcaaccagt ccagaaacga aacatagcga
     5401 gtttttggcc aacgtcagca ccatcacctc agattattcc accacatcgt ctgctacata
     5461 cttgactagc ctggactcca gtcgactgag ccctgaggtg caatccgtgg cagagagcaa
     5521 gggggacgag gcagatgacg agagaagcga actcatcagt gaagggcggc ctgtggaaac
     5581 cgacagcgag agcgagtttc ccgtgttccc cacagccttg acttcagaga ggcttttccg
     5641 aggaaaactg caagaagtga ctaagagcag ccggagaaat tctgaaggaa gtgaattaag
     5701 ttgcaccgag ggaagtttaa catcaagttt agatagccgg agacagctct tcagttccca
     5761 taaactcatc gaatgtgata ctctttccag gaaaaaatca gctagattca agtcagatag
     5821 tggaagtcta ggagatgcca agaatgagaa agaagcacct tcgttaacta aagtgtttga
     5881 tgttatgaaa aaaggaaagt caactgggag tttactgaca cccaccagag gcgaatccga
     5941 aaaacaggaa cccacatgga aaacgaaaat agcagatcgg ttaaaactga gacccagagc
     6001 ccctgcggat gacatgtttg gagtagggaa tcacaaagtg aatgccgaga ctgctaaaag
     6061 gaaaagcatc cggcgcagac atacactagg agggcacaga gatgctaccg aaatcagcgt
     6121 tttgaatttt tggaaagtgc atgagcagag cggggagaga gaatctgaac tttcagctgt
     6181 aaaccggtta aaaccaaaat gctcagccca ggacctttcc atctcagact ggctggccag
     6241 ggaacgccta cgcaccagta cctctgacct tagcagagga gaaatcggag atccccagac
     6301 agagaaccca agcacacgag aaatagccac gaccgacaca cctttgtctc ttcattgcaa
     6361 cacaggcagt tcttccagca ccttggcttc aacaaacagg ccccttcttt ccataccacc
     6421 acagtcacct gaccaaataa acggagaaag cttccagaac gtgagcaaaa atgctagttc
     6481 tgcagcgaat gcccaacctc ataaactgtc tgaaacccca ggcagtaaag cagagtttca
     6541 tccctgtctt taaactgggg gtatgtccac tctagcaagt aaaaaaacta ctgttacacg
     6601 ttccagtaac tctgtcaata ttttcttgta tcagaattgt tattatgcag ccttcatttg
     6661 ggctggtttc atcattttgc actgtgaaat agctttacag tgcattacta cagccagaag
     6721 aacatatata tatatatata tatttaaaaa tatatcggat agttgtatac aaatgagcaa
     6781 ggtatttgtt gcaacttact acatagcata tacccaaaat cactgaagaa aatcgctggc
     6841 atcagtgtgc agcaaatttg ttcttttggt ttcatcacta acaaaagtgc ctcatcataa
     6901 aaatacagtt ggtttttagg gtgccatatt gttaaaatta gataacttac ttacattgaa
     6961 taaacgaatg cgttttattg gtaacagata tcattacatt taccagtttt aacacaggtg
     7021 gatacagaac ttccattctt tagtcattcc aggtggatct gagttttata ttcaaacttt
     7081 taatacagtt tttgagtttt gtgtgacttg aatttttaat ctttctgtaa aatacgtaac
     7141 ttaaatgaac atattaaatg tgtatctttt cttcagatac cagatttgat ataatgttgt
     7201 aacataggtg tgtagatagt ggatcctgga tggaactggc ttctttatcg agaagaatat
     7261 aattctgcat gaggacttaa tgaatccaaa cctgtgtcat gcctgtgtgc atacccaatt
     7321 aaacactgga aataaaaatt gttttggtga a
//
