LOCUS       NM_006420               9031 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens ARF guanine nucleotide exchange factor 2 (ARFGEF2),
            transcript variant 1, mRNA.
ACCESSION   NM_006420
VERSION     NM_006420.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 9031)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 9031)
  AUTHORS   Lu,F.I., Wang,Y.T., Wang,Y.S., Wu,C.Y. and Li,C.C.
  TITLE     Involvement of BIG1 and BIG2 in regulating VEGF expression and
            angiogenesis
  JOURNAL   FASEB J 33 (9), 9959-9973 (2019)
   PUBMED   31199673
  REMARK    GeneRIF: BIG1 and BIG2 knockdown significantly decreased the levels
            of VEGF mRNA and protein in glioblastoma U251 cells and HUVECs.
            Furthermore, depletion of BIG1 and BIG2 inhibited HUVEC
            angiogenesis by diminishing cell migration.
REFERENCE   4  (bases 1 to 9031)
  AUTHORS   Li,C.C., Le,K., Kato,J., Moss,J. and Vaughan,M.
  TITLE     Enhancement of beta-catenin activity by BIG1 plus BIG2 via Arf
            activation and cAMP signals
  JOURNAL   Proc Natl Acad Sci U S A 113 (21), 5946-5951 (2016)
   PUBMED   27162341
  REMARK    GeneRIF: Both phospholipase D activity and vesicular trafficking
            were required for effects of BIG1 and BIG2 on beta-catenin
            activation. Levels of PKA-phosphorylated beta-catenin S675 and
            beta-catenin association with PKA, BIG1, and BIG2 were also
            diminished after BIG1/BIG2 depletion.
REFERENCE   5  (bases 1 to 9031)
  AUTHORS   Mick,E., McGough,J., Deutsch,C.K., Frazier,J.A., Kennedy,D. and
            Goldberg,R.J.
  TITLE     Genome-wide association study of proneness to anger
  JOURNAL   PLoS One 9 (1), e87257 (2014)
   PUBMED   24489884
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9031)
  AUTHORS   Shinotsuka,C., Yoshida,Y., Kawamoto,K., Takatsu,H. and Nakayama,K.
  TITLE     Overexpression of an ADP-ribosylation factor-guanine nucleotide
            exchange factor, BIG2, uncouples brefeldin A-induced adaptor
            protein-1 coat dissociation and membrane tubulation
  JOURNAL   J Biol Chem 277 (11), 9468-9473 (2002)
   PUBMED   11777925
REFERENCE   7  (bases 1 to 9031)
  AUTHORS   Pacheco-Rodriguez,G., Moss,J. and Vaughan,M.
  TITLE     BIG1 and BIG2: brefeldin A-inhibited guanine nucleotide-exchange
            proteins for ADP-ribosylation factors
  JOURNAL   Methods Enzymol 345, 397-404 (2002)
   PUBMED   11665623
  REMARK    GeneRIF: involvement in molecular mechanisms of vesicular transport
REFERENCE   8  (bases 1 to 9031)
  AUTHORS   Kamei,Y., Takeda,Y., Teramoto,K., Tsutsumi,O., Taketani,Y. and
            Watanabe,K.
  TITLE     Human NB-2 of the contactin subgroup molecules: chromosomal
            localization of the gene (CNTN5) and distinct expression pattern
            from other subgroup members
  JOURNAL   Genomics 69 (1), 113-119 (2000)
   PUBMED   11013081
REFERENCE   9  (bases 1 to 9031)
  AUTHORS   Yamaji,R., Adamik,R., Takeda,K., Togawa,A., Pacheco-Rodriguez,G.,
            Ferrans,V.J., Moss,J. and Vaughan,M.
  TITLE     Identification and localization of two brefeldin A-inhibited
            guanine nucleotide-exchange proteins for ADP-ribosylation factors
            in a macromolecular complex
  JOURNAL   Proc Natl Acad Sci U S A 97 (6), 2567-2572 (2000)
   PUBMED   10716990
REFERENCE   10 (bases 1 to 9031)
  AUTHORS   Togawa,A., Morinaga,N., Ogasawara,M., Moss,J. and Vaughan,M.
  TITLE     Purification and cloning of a brefeldin A-inhibited guanine
            nucleotide-exchange protein for ADP-ribosylation factors
  JOURNAL   J Biol Chem 274 (18), 12308-12315 (1999)
   PUBMED   10212200
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL049537.48, BC050449.1,
            AK074303.1, AB209397.1 and AL121903.13.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_006420.2.
            
            Summary: ADP-ribosylation factors (ARFs) play an important role in
            intracellular vesicular trafficking. The protein encoded by this
            gene is involved in the activation of ARFs by accelerating
            replacement of bound GDP with GTP and is involved in Golgi
            transport. It contains a Sec7 domain, which may be responsible for
            its guanine-nucleotide exchange activity and also brefeldin A
            inhibition. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.230691.1, AF084521.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000371917.5/ ENSP00000360985.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                AL049537.48        32211-32237
            28-2664             BC050449.1         1-2637
            2665-3841           AK074303.1         14-1190
            3842-5689           AB209397.1         771-2618
            5690-9031           AL121903.13        33924-37265
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.13"
     gene            1..9031
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /note="ARF guanine nucleotide exchange factor 2"
                     /db_xref="GeneID:10564"
                     /db_xref="HGNC:HGNC:15853"
                     /db_xref="MIM:605371"
     exon            1..300
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     CDS             180..5537
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     brefeldin A-inhibited guanine nucleotide-exchange protein
                     2; brefeldin A-inhibited GEP 2; ADP-ribosylation factor
                     guanine nucleotide-exchange factor 2 (brefeldin
                     A-inhibited); ADP ribosylation factor guanine nucleotide
                     exchange factor 2"
                     /codon_start=1
                     /product="brefeldin A-inhibited guanine
                     nucleotide-exchange protein 2 isoform 1"
                     /protein_id="NP_006411.2"
                     /db_xref="CCDS:CCDS13411.1"
                     /db_xref="GeneID:10564"
                     /db_xref="HGNC:HGNC:15853"
                     /db_xref="MIM:605371"
                     /translation="MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIK
                     AEIEKQRLGTAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITG
                     NAPDSGAPGKRLIDRIVETICSCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTIL
                     QTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPIQSKPQS
                     PVIQAAAVSPKFVRLKHSQAQSKPTTPEKTDLTNGEHARSDSGKVSTENGDAPRERGS
                     SLSGTDDGAQEVVKDILEDVVTSAIKEAAEKHGLTEPERVLGELECQECAIPPGVDEN
                     SQTNGIADDRQSLSSADNLESDAQGHQVAARFSHVLQKDAFLVFRSLCKLSMKPLGEG
                     PPDPKSHELRSKVVSLQLLLSVLQNAGPVFRTHEMFINAIKQYLCVALSKNGVSSVPD
                     VFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICA
                     DAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLEC
                     LVSILKCMVEWSKDLYVNPNHQTSLGQERLTDQEIGDGKGLDMARRCSVTSMESTVSS
                     GTQTTVQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTSVEDIAQFL
                     HQEERLDSTQVGDFLGDSARFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQ
                     KIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYI
                     KMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKELTIATKSTKQNVASEKQRRLLY
                     NLEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDD
                     TEVASLCLEGIRCAIRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIK
                     TLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTL
                     AGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIV
                     DFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVG
                     CNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMAI
                     RCIAQMVNSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTCHIVTTIFQHHFPA
                     AIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAP
                     GDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWQDLFRIV
                     FRIFDNMKLPEQLSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSDVFAQLQWCV
                     KQDNEQLARSGTNCLENLVISNGEKFSPEVWDETCNCMLDIFKTTIPHVLLTWRPVGM
                     EEDSSEKHLDVDLDRQSLSSIDKNPSERGQSQLSNPTDDSWKGRPYANQKLFASLLIK
                     CVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETEDQGMYKYMSSQH
                     LFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFR
                     MYVDENRRDSWEEIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKINDE
                     KFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGVVYKIWIPEEPSQVPAALSPV
                     W"
     exon            301..331
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            332..455
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            456..602
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            603..782
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            783..1017
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1086
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1238
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1369
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1604
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1704
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1844
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..1953
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1954..2137
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2138..2249
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2250..2455
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2456..2540
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2541..2712
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2713..2864
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2865..2993
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2994..3152
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            3153..3300
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            3301..3400
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            3401..3441
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            3442..3611
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            3612..3763
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            3764..3936
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            3937..4097
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4098..4228
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4229..4358
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4359..4494
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4495..4633
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4634..4688
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4689..4803
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4804..4934
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4935..5103
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5104..5242
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5243..5360
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5361..9031
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5934..5939
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /note="hexamer: AATAAA"
     polyA_site      5970
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
     regulatory      9013..9018
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /note="hexamer: AATAAA"
     polyA_site      9031
                     /gene="ARFGEF2"
                     /gene_synonym="BIG2; dJ1164I10.1; PVNH2"
                     /note="major polyA site"
ORIGIN      
        1 acgtggtcac gtgacgcgct ccaacatggc ggcgccgtgg ggccgaggtg tcgcttcctg
       61 acggggcggc gcggacggac gcggccggtg ccggccggga cgccgggccc gcagcctagc
      121 tcgccatctc gctcacgccg cccgcccgcg gggccgtcag cccccgccgg gccggggcca
      181 tgcaggagag ccagaccaag agcatgttcg tgtcccgggc cctggagaag atcctagccg
      241 acaaggaggt gaagcggccc cagcactccc agctgcgcag ggcctgccag gtggcgctcg
      301 atgaaattaa agcagaaata gaaaagcaga ggcttggcac tgctgcacca ccaaaggcaa
      361 acttcattga agctgacaag tattttcttc cattcgagct agcttgccag tccaagtccc
      421 caagggtagt cagcacatcc cttgactgct tgcagaaact catcgcatac gggcacatca
      481 ctggcaacgc ccctgacagt ggagcccctg ggaagcggct gatcgacaga attgttgaaa
      541 ccatttgcag ttgttttcag ggccctcaga ctgatgaagg ggttcagtta caaataatta
      601 aggctcttct gactgcagtg acttccccac acattgaaat tcatgagggt actatcctgc
      661 agacagtgag aacatgttac aatatctatt tggccagcaa aaatctcatc aatcaaacca
      721 ctgccaaggc tacccttact cagatgctga acgtcatttt cacccgcatg gaaaaccaag
      781 tgttgcagga ggccagagaa ctggaaaaac caatccagtc aaaaccccag tcccctgtga
      841 tccaagctgc agcagtatcc ccaaagttcg ttcgtttgaa gcacagtcag gcacaaagca
      901 aaccaacaac tcccgaaaaa acagatttaa ccaacggtga acatgccagg agtgattctg
      961 gaaaagtaag cacagaaaat ggagacgcac ccagagaaag aggctcatca ctgtcaggga
     1021 ctgatgacgg agcccaggag gtggtgaagg acatcttgga agatgtagtc acatctgcca
     1081 ttaaagaagc agcggaaaag catggtctga cagaacctga gagagttcta ggtgaactgg
     1141 agtgccagga atgtgctatt cccccaggag ttgatgaaaa ctcacagacc aacgggatag
     1201 ccgatgacag gcagtccttg tcgtcagcag ataatctgga atcggatgca caaggacatc
     1261 aagtggctgc caggttctcc cacgttctgc agaaggatgc cttccttgtg ttccgctccc
     1321 tgtgcaagct gtccatgaaa ccccttggtg aaggccctcc agacccaaaa tcccatgagc
     1381 tgcgttccaa ggtggtttcc ctgcagctgc tcctctctgt gttgcaaaat gctggccccg
     1441 tattcaggac tcacgagatg ttcatcaatg caatcaagca atatctctgt gtggccttgt
     1501 ccaaaaacgg cgtctcttca gtgcctgatg tctttgagct ctctcttgcc atttttctta
     1561 ctcttctttc aaactttaaa atgcacttga aaatgcagat agaggtcttt ttcaaagaga
     1621 ttttcctgaa cattttagaa acatcaacaa gttcttttga gcacaggtgg atggtcattc
     1681 agactctgac gaggatctgt gcagatgccc agtgtgttgt ggatatttat gtcaactacg
     1741 actgtgattt aaatgctgct aacatttttg agcgccttgt aaatgattta tccaaaattg
     1801 ctcagggaag aagtggacat gagctgggaa tgacacctct gcaggagctc agcctgagga
     1861 agaaaggcct ggagtgcctc gtgtccattc tcaagtgcat ggtggagtgg agcaaagacc
     1921 tgtatgtgaa tcccaaccac cagaccagcc tcggtcagga gaggctcacg gatcaggaaa
     1981 taggggatgg gaaaggcctt gacatggcaa gacggtgtag tgtgacgtcc atggagtcca
     2041 cagtgtcctc ggggacccag acaactgttc aggatgaccc tgagcaattt gaggtcatca
     2101 agcaacaaaa agaaatcatt gaacacggca tcgagctgtt caacaagaaa cccaagaggg
     2161 ggatccagtt tctccaggag cagggcatgc tgggaacgtc agttgaagac atagcccaat
     2221 tcctgcacca ggaggagcgc ctggattcca cccaagtagg cgattttctg ggagatagcg
     2281 caaggttcaa caaggaggtg atgtatgcct acgtggacca acttgacttc tgtgaaaaag
     2341 aatttgtctc agccctgcgg acattcctag aaggtttccg cctacctgga gaagcccaaa
     2401 agattgaccg attaatggag aagtttgccg caagatacat agaatgcaac caagggcaaa
     2461 ctctgtttgc tagtgctgac actgcttatg tcctagcgta ttcaattatt atgctgacta
     2521 cagacttgca cagtcctcag gtaaaaaata aaatgacgaa agagcagtat attaaaatga
     2581 atcggggtat caatgatagt aaagatctgc cagaagagta tctctcaagc atctatgaag
     2641 agatagaagg caagaaaatt gcaatgaaag aaacaaaaga gctaacgatt gcaaccaaat
     2701 ctactaagca gaatgtagct agtgaaaagc agcggcggct gctgtacaac ttagagatgg
     2761 agcaaatggc taaaacagcc aaagctctga tggaggctgt gagccatgcc aaagccccgt
     2821 ttaccagtgc cactcacctg gaccatgtcc ggccaatgtt caaactggtg tggacgccac
     2881 tattggcagc ctacagcatc ggactccaga actgtgatga cactgaagtg gcctccttgt
     2941 gtttggaagg catccgatgt gcaatccgaa tcgcctgcat ctttggaatg cagctggaac
     3001 gagatgccta tgttcaggct cttgctcgct tctccctact cacagccagc tccagcatca
     3061 cagaaatgaa gcagaaaaac atcgacacca ttaagacgct tatcacagtg gctcacaccg
     3121 atggcaacta ccttgggaat tcctggcatg agatcttgaa atgcatcagc cagctggagc
     3181 tcgctcagct gataggaacc ggtgtgaaga cgcgctacct gtctggatct gggcgtgaaa
     3241 gagaagggag cctgaagggc cacacattgg caggagaaga gttcatgggc cttggcctcg
     3301 gtaatttggt gagtggcgga gtggataaaa gacagatggc cagcttccaa gaatcggttg
     3361 gtgagaccag ctcgcagagt gtggttgtag ctgtggacag gatttttact gggtctacca
     3421 gactggatgg aaatgcaata gttgactttg tccgctggct gtgtgctgtg tccatggatg
     3481 aactggcttc cccccaccat cctcgcatgt tcagcttgca gaagattgtg gagatatcat
     3541 actacaacat gaatcggatc cgactacagt ggtctcgaat atggcatgtg attggagatc
     3601 acttcaataa ggttggctgc aaccctaatg aagatgtggc tatctttgct gttgactcat
     3661 taaggcaact ctccatgaag tttcttgaga agggtgaatt agccaacttc cgtttccaga
     3721 aagattttct gaggcccttt gagcatatta tgaagaaaaa caggtctccc accatccggg
     3781 acatggcgat ccgctgcatt gcccagatgg tgaactccca ggcggccaac atccgctcag
     3841 gttggaagaa catctttgcc gtgttccacc aggcagcctc tgatcatgat gggaacattg
     3901 tggagctggc cttccagacc acttgccaca ttgtcacaac tattttccag caccattttc
     3961 ctgcagccat cgattccttt caggatgctg tgaagtgctt atcagagttc gcctgcaacg
     4021 ccgctttccc tgacacgagc atggaagcga ttcggctcat ccgcttctgt ggcaaatacg
     4081 tctctgagag gcctcgggtg ctacaagaat acacaagtga tgacatgaat gtagctcctg
     4141 gtgacagagt ctgggtccga ggctggttcc ccatcttatt cgaactctcc tgcatcatta
     4201 atagatgcaa gttagatgta cgaacaaggg gactcacagt catgtttgag atcatgaaga
     4261 gctatggcca cacctttgaa aagcactggt ggcaggacct gttcagaatc gtgtttcgga
     4321 tttttgacaa tatgaaactc cctgagcaac tgtcagagaa atctgagtgg atgacaacaa
     4381 cctgcaatca cgcactttat gctatttgtg atgtttttac ccagttttat gaagctttga
     4441 atgaagttct tctttctgat gtatttgcac aattgcagtg gtgtgtcaaa caagataatg
     4501 aacagttggc gcgatcaggt acaaattgct tagaaaactt agtaatatcc aatggagaga
     4561 aattcagtcc tgaagtctgg gatgaaacct gcaactgtat gttggatatt ttcaaaacaa
     4621 ccatcccaca tgttttgctg acatggagac ctgtaggaat ggaggaagat tcatcagaaa
     4681 agcatttgga tgtggatctg gaccgccagt ctttaagcag catagataaa aatccctctg
     4741 agaggggaca gagccagctc tctaacccaa cagatgacag ctggaagggt agaccatacg
     4801 caaatcagaa actgtttgcc agcctcctca tcaagtgtgt ggtccagttg gaattgatac
     4861 agaccattga caacattgtg ttctaccctg cgacgagcaa aaaggaggat gcagagcaca
     4921 tggttgccgc ccagcaagac acgctggatg cagatatcca catagagacg gaggatcagg
     4981 gcatgtataa gtacatgtct tcccagcacc tcttcaagct gttggactgt ttgcaggaat
     5041 cccattcatt ctcaaaggcc ttcaactcca attacgagca gcggactgtc ctgtggcgag
     5101 caggttttaa gggcaagtct aaacccaatc ttctaaaaca agaaaccagc agcctggcct
     5161 gttgtttgag gatcctgttt cgaatgtatg ttgatgagaa ccgcagggat tcctgggaag
     5221 aaatacagca gagactttta actgtttgca gtgaagctct tgcctatttc atcactgtga
     5281 attctgagag ccatcgggag gcctggacaa gtctcttgtt gttacttcta actaaaaccc
     5341 tcaaaataaa tgatgaaaag ttcaaagcac atgcttcaat gtactacccc tacttgtgtg
     5401 aaattatgca gtttgacctg atccctgagc tccgagcagt tctgcggaag ttcttcctac
     5461 ggataggtgt tgtgtataag atatggatac cagaagagcc atcacaggta ccagcagcac
     5521 tgtcaccagt gtggtagccc tggctgccca ggccagtgct gcagctctgc agaatgttca
     5581 gcatgccatt tctgactggc acatctcgtg aagtttcata gaaacaagga gttggcatct
     5641 tggatctcag aatggcctgg aaacggatgg cctctacgct gttccatcac agtctccaac
     5701 taaggcttat ggtatttcat taaactgttg catacccagt tagcacagta ggtggggagt
     5761 ctgcttcatt tctatcattc catttttctg attaaactgt caaatctgtc attgcatatg
     5821 ccatcgtttt ctagcaaaat cccatgattg gctataaacg ttttgtaaga agtcactctc
     5881 cttgaaaata ctgaacatag ctgtataggt ttgtgattat tagagaatat gttaataaaa
     5941 cttcttgtaa cggctaactg ccacctaaaa tatgctgggt tttctgttgt ttgagtgtgt
     6001 tagagaaatt tgaatgtttt tgtcagttac gagtcagccg taattagatt agtttaagga
     6061 caggtcagga ttagagaaga gttgtttttg tgttttgtta atgtctgagt gatttttaaa
     6121 gtattttaca aaaagatatt gaaaatttgg ttgaaggcag agtttagtaa ttaagttaga
     6181 attaagagtt ttgcgaggtt aaaaaaatgt gcctcgtgga tctccctgtt ttagtaacat
     6241 ggagagaaaa agtctacacg aaaaagtgaa caatttaatg aagatgatta gccttccttg
     6301 aaataagtat ttgtggatgg gtgttaaatt aaaatttcca gaatacactg tccatctcac
     6361 acactctgaa atctaatata tgaagtagta atgaaaatga agtagtaatt taaccagagt
     6421 tcatttatcc ttgaataaac ctttttattt tcacctcaga aaagtgagtg tactggcagt
     6481 tagtgtcact gctttgcaca gtccccatta aaggaccctc cagagaggga cagtaactgt
     6541 gcatgagaag ccgctcccat aagcctcctc agccagatgt catgggtgga actggagctg
     6601 tgtcggggcc agcacagctg aactgtgaca atggcaggag gtggcatgtg cccagcactt
     6661 ccattaatct gagcctagga gttgaattct ttggcaaggt tggattctga ggtccttatt
     6721 atgttaatga tggtgcaata ctctcacctg cagtagaact gagttctgct gcagcttgtg
     6781 taaaagtggg cagtacacaa gtacgtccca aagcctgtga gcagtatacg tggatgctca
     6841 cccatgagaa ggagcacaca cgcctcattc tctgccctca cccactgctc acctagagca
     6901 tcgctgagcg ttagacaaag tgttacacag aatgattaaa actttcagac ttctacctat
     6961 gctctttagt cctgtaaatt gggttgtatt gatgtcaact ctggtgcctt agaagttagt
     7021 agtttgggaa cctatctgta aaatcagatg ttttttcttt gtagagaagg atttctggtg
     7081 cttttgctta ctaagagacc gatattctta agttgttttc ttgttttaac agccttgaga
     7141 aatgtttggt tttggccagc agaattcttg tctacttttt tctttcccaa aaagtgtttt
     7201 ttaatttctc taccaaagaa aaaatgagca ggtttaggtt tttacatgac ttatatacat
     7261 tagataaaag gagctgtata atttagcagg aagggactat gggagaatac tttactgtga
     7321 gtggaaaatg ttagcacatt tgactggttt gccctggaat ccactgcacc tttacactgc
     7381 accatgaaac ctacactccc tggtatcata gcgcgtcatc acctcaacaa gtcagtcgtc
     7441 tccattgata tttgtacaaa aggtatacat ggggaacacg tgttcattca ttaagtccat
     7501 cttgcgtgca gctatatccc tgattggtta tttttccttt ccttctgagg ttctcatgtc
     7561 attttcttca tcggatgtga ctaaaatttt tctggtgtct tctgccctct ctttaatttt
     7621 gcctcttgag gggtagcaga tgtgtcagtg cattttatta cttgctgaaa cattcaggct
     7681 tacatttctt attagtttag tattttaaaa gatttaattt tctgaatgag gcatttgaat
     7741 tgtaccagca atggactttt aaaaaattgg atgtaaaacc attcagggtg atttttcttg
     7801 tcagtggaca gtgacgaaca gagatttgaa atcccttacc tccaataata agccattcag
     7861 cctaaattca tttttatgaa taaatctctt ctttctcatg gtaaatgtgg cttgtgccac
     7921 tcaaacacta gtgaaagggt atgtacaacc gcaacatcag gccaggacac catttattta
     7981 accaagtaat ggaggagagt gaaacatttt ccaaggcctt atttcttttt cagaatgctt
     8041 taagtgttga ttatatgtgc tgggtctcta gagaagtttt tatttgttac aatactgctg
     8101 ctttgagcaa ttttgtttct cttcattgtc tgttagggaa atcaccagct ttggcatctt
     8161 aacaacaaac agtggatggg taatttttat ttctgatacg catctttaga gtcaaatata
     8221 tcttttccct gtactcctca tgtacaacca aagaacatac attatgaaaa ttgtattata
     8281 atatctggaa acacaacatt ttcctctggc aggtgacttt tgtacgaaat gaaaaaaaaa
     8341 aaacctgtat tttttttgtt gttcttttgt gattaaggat atacatttag tagtctttgt
     8401 tattaaagga acctgctgat aagtacaagt gtgaccatct cattcaaatg tttgttgtta
     8461 tgatgcaaat ggatattggt ttcaatagaa gctggatcct taatactgca ttttctgaat
     8521 tctgttttaa tatttgtgtt aaagtagaca tagctgaact cacatggaat cctggagttt
     8581 tgttatcagc agctttgcag tttgggaaac aaagaaacca aagctgagtg ttttaaagaa
     8641 tgaacatatc tggaaatcct tgctctcaaa acaaaacaat ataatctatc aaaggtgttt
     8701 tactcagtgt tctaattttt aaaaaatttt atctgcatat ttgcatcaaa tatttatgtg
     8761 taaaatgtat gttttacctc cttaaaatgc ctaaaagtta gttaatagtt actttggttt
     8821 aacctataca caatgattaa gtgcatttaa tattggtaat ttaatgaaaa ccattccttg
     8881 cccaatggat gtataaattt ttgaaagaat aagcagaatt atataatatg aaatgctatg
     8941 taaagttttc tatgtaaaaa tattatgtat aatactatta aaatatccca tgctttgatc
     9001 aggtggtaca tcaataaaat tttaaaaagt a
//
