LOCUS       NM_006421               7320 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens ARF guanine nucleotide exchange factor 1 (ARFGEF1),
            transcript variant 1, mRNA.
ACCESSION   NM_006421
VERSION     NM_006421.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Jiang,L. and Wang,X.
  TITLE     The miR-133b/brefeldin A-inhibited guanine nucleotide-exchange
            protein 1 (ARFGEF1) axis represses proliferation, invasion, and
            migration in cervical cancer cells
  JOURNAL   Bioengineered 13 (2), 3323-3332 (2022)
   PUBMED   35048795
  REMARK    GeneRIF: The miR-133b/brefeldin A-inhibited guanine
            nucleotide-exchange protein 1 (ARFGEF1) axis represses
            proliferation, invasion, and migration in cervical cancer cells.
REFERENCE   3  (bases 1 to 7320)
  AUTHORS   Thomas,Q., Gautier,T., Marafi,D., Besnard,T., Willems,M.,
            Moutton,S., Isidor,B., Cogne,B., Conrad,S., Tenconi,R., Iascone,M.,
            Sorlin,A., Masurel,A., Dabir,T., Jackson,A., Banka,S., Delanne,J.,
            Lupski,J.R., Saadi,N.W., Alkuraya,F.S., Zahrani,F.A., Agrawal,P.B.,
            England,E., Madden,J.A., Posey,J.E., Burglen,L., Rodriguez,D.,
            Chevarin,M., Nguyen,S., Mau-Them,F.T., Duffourd,Y., Garret,P.,
            Bruel,A.L., Callier,P., Marle,N., Denomme-Pichon,A.S., Duplomb,L.,
            Philippe,C., Thauvin-Robinet,C., Govin,J., Faivre,L. and
            Vitobello,A.
  TITLE     Haploinsufficiency of ARFGEF1 is associated with developmental
            delay, intellectual disability, and epilepsy with variable
            expressivity
  JOURNAL   Genet Med 23 (10), 1901-1911 (2021)
   PUBMED   34113008
  REMARK    GeneRIF: Haploinsufficiency of ARFGEF1 is associated with
            developmental delay, intellectual disability, and epilepsy with
            variable expressivity.
REFERENCE   4  (bases 1 to 7320)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 7320)
  AUTHORS   Liu,L., Zhang,S., Wang,Y., Bao,W., Zhou,Y., Dang,W., Wang,X.,
            Li,H., Cao,X., You,Y., Fang,H. and Shen,X.
  TITLE     BIG1 controls macrophage pro-inflammatory responses through
            ARF3-mediated PI(4,5)P2 synthesis
  JOURNAL   Cell Death Dis 11 (5), 374 (2020)
   PUBMED   32415087
  REMARK    GeneRIF: BIG1 controls macrophage pro-inflammatory responses
            through ARF3-mediated PI(4,5)P2 synthesis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7320)
  AUTHORS   Kamei,Y., Takeda,Y., Teramoto,K., Tsutsumi,O., Taketani,Y. and
            Watanabe,K.
  TITLE     Human NB-2 of the contactin subgroup molecules: chromosomal
            localization of the gene (CNTN5) and distinct expression pattern
            from other subgroup members
  JOURNAL   Genomics 69 (1), 113-119 (2000)
   PUBMED   11013081
REFERENCE   7  (bases 1 to 7320)
  AUTHORS   Yamaji,R., Adamik,R., Takeda,K., Togawa,A., Pacheco-Rodriguez,G.,
            Ferrans,V.J., Moss,J. and Vaughan,M.
  TITLE     Identification and localization of two brefeldin A-inhibited
            guanine nucleotide-exchange proteins for ADP-ribosylation factors
            in a macromolecular complex
  JOURNAL   Proc Natl Acad Sci U S A 97 (6), 2567-2572 (2000)
   PUBMED   10716990
REFERENCE   8  (bases 1 to 7320)
  AUTHORS   Mansour,S.J., Skaug,J., Zhao,X.H., Giordano,J., Scherer,S.W. and
            Melancon,P.
  TITLE     p200 ARF-GEP1: a Golgi-localized guanine nucleotide exchange
            protein whose Sec7 domain is targeted by the drug brefeldin A
  JOURNAL   Proc Natl Acad Sci U S A 96 (14), 7968-7973 (1999)
   PUBMED   10393931
REFERENCE   9  (bases 1 to 7320)
  AUTHORS   Togawa,A., Morinaga,N., Ogasawara,M., Moss,J. and Vaughan,M.
  TITLE     Purification and cloning of a brefeldin A-inhibited guanine
            nucleotide-exchange protein for ADP-ribosylation factors
  JOURNAL   J Biol Chem 274 (18), 12308-12315 (1999)
   PUBMED   10212200
REFERENCE   10 (bases 1 to 7320)
  AUTHORS   Morinaga,N., Tsai,S.C., Moss,J. and Vaughan,M.
  TITLE     Isolation of a brefeldin A-inhibited guanine nucleotide-exchange
            protein for ADP ribosylation factor (ARF) 1 and ARF3 that contains
            a Sec7-like domain
  JOURNAL   Proc Natl Acad Sci U S A 93 (23), 12856-12860 (1996)
   PUBMED   8917509
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC021321.11, AI692863.1,
            AF111162.1, AL117446.1 and CA314908.1.
            
            On Nov 24, 2018 this sequence version replaced NM_006421.4.
            
            Summary: ADP-ribosylation factors (ARFs) play an important role in
            intracellular vesicular trafficking. The protein encoded by this
            gene is involved in the activation of ARFs by accelerating
            replacement of bound GDP with GTP. It contains a Sec7 domain, which
            may be responsible for guanine-nucleotide exchange activity and
            also brefeldin A inhibition. [provided by RefSeq, Aug 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.152129.1,
                                           SRR1803614.267959.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262215.8/ ENSP00000262215.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-104               AC021321.11        153347-153450       c
            105-476             AI692863.1         1-372
            477-6503            AF111162.1         124-6150
            6504-6698           AL117446.1         1103-1297
            6699-7320           CA314908.1         24-645              c
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q13.2"
     gene            1..7320
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /note="ARF guanine nucleotide exchange factor 1"
                     /db_xref="GeneID:10565"
                     /db_xref="HGNC:HGNC:15772"
                     /db_xref="MIM:604141"
     exon            1..618
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     CDS             495..6044
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /note="isoform 1 is encoded by transcript variant 1;
                     brefeldin A-inhibited guanine nucleotide-exchange protein
                     1; p200 ARF guanine nucleotide exchange factor; ADP
                     ribosylation factor guanine nucleotide exchange factor 1;
                     ADP-ribosylation factor guanine nucleotide-exchange factor
                     1 (brefeldin A-inhibited)"
                     /codon_start=1
                     /product="brefeldin A-inhibited guanine
                     nucleotide-exchange protein 1 isoform 1"
                     /protein_id="NP_006412.2"
                     /db_xref="CCDS:CCDS6199.1"
                     /db_xref="GeneID:10565"
                     /db_xref="HGNC:HGNC:15772"
                     /db_xref="MIM:604141"
                     /translation="MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEI
                     KAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDC
                     LQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVT
                     SQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAK
                     QMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSL
                     QDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVE
                     EMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDR
                     LSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHEL
                     RSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIF
                     LTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIY
                     VNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMV
                     EWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETINRYGSLNSLESTSSSGIGSYSTQ
                     MSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQE
                     ERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKID
                     RLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMN
                     RGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQNVASEKQRRLLYNLE
                     MEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEV
                     ASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLI
                     TVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAP
                     DEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRW
                     LCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNE
                     DVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQ
                     MVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSF
                     QDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVW
                     VRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFD
                     NMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNE
                     QLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPNSGETAPP
                     PPSPVSEKPLDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPE
                     QKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQ
                     GMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSS
                     LACGLRILFRMYMDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLF
                     LTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQ
                     ELGINKQ"
     exon            619..649
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            650..806
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            807..953
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1133
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1410
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1521
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1697
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..1831
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..2066
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..2166
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2306
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            2307..2415
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            2416..2617
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            2618..2729
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            2730..2935
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            2936..3020
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3021..3192
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3193..3344
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3345..3473
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3474..3632
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3633..3783
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3784..3874
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3875..3915
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3916..4085
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            4086..4237
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            4238..4410
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            4411..4571
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            4572..4702
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            4703..4832
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            4833..4968
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            4969..5107
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            5108..5180
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            5181..5313
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            5314..5453
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            5454..5622
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            5623..5761
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            5762..5879
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            5880..7320
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6595..6600
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /note="hexamer: TATAAA"
     polyA_site      6615
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
     regulatory      7283..7288
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /note="hexamer: AATAAA"
     polyA_site      7320
                     /gene="ARFGEF1"
                     /gene_synonym="ARFGEP1; BIG1; DEDISB; P200"
                     /note="major polyA site"
ORIGIN      
        1 gtttcctccc aacatggcgg ccggggcagg tcggcggtga tgggggccag ttcgcggccg
       61 cggcgggtgc taatccccac agtagccagg gtagcccttc tctcgcggtg cctctgagag
      121 ccgccgccac ctcgtccctc agttcccggc agacagccgc ccgggccggg ctccctcacc
      181 gccaccaggg ccggagggac gagtgagagg ggcggccggg acgcgacccc cgggccggga
      241 gaagcggagg cccgacgggc gcggccctcg aggtacggct tcttgggggc ggcgaggacc
      301 tcgacgccct tcgccgacac cgcggctggt cccgctcgcc cgccccttcc tgatcctgcc
      361 gctgccctcc acgcctccaa tcccccacct ccaccccctc tcctttctct cttctcttcc
      421 ttcctctcct cctcctcccc tccagccgct gggagccgcg ggtcggacga gccgccgcct
      481 ccttctctgc gtccatgtat gaggggaaga agacgaagaa catgttcctg acccgggctc
      541 tggagaagat attggccgac aaggaagtga agaaggcgca tcactcccag ctgcgcaaag
      601 cttgcgaggt ggcgttagag gaaataaaag cggaaactga aaaacagagt cctcctcatg
      661 gagaagcaaa agctggatca agcacccttc caccagtgaa atcaaagaca aattttattg
      721 aagcagacaa gtacttcttg ccttttgagt tggcatgcca gtccaaatgt cctcgcatag
      781 ttagtacatc tctagattgc ttacagaaac ttattgctta tgggcacttg actggcaatg
      841 ctccagatag tacaacacca ggcaaaaaat taattgatag aattattgaa acaatatgtg
      901 gctgctttca gggccctcag acagatgaag gagttcagct gcagataata aaggctttac
      961 ttactgcagt aacatcacaa cacatagaaa ttcatgaagg gactgtactg caagctgtga
     1021 gaacatgtta caatatctac ttagcaagca aaaatctcat caatcagaca acagccaaag
     1081 ctactctcac tcagatgcta aatgttatct ttgcacgcat ggaaaaccaa gcattacagg
     1141 aagcaaaaca aatggaaaaa gaaaggcatc ggcagcatca tcatctgtta cagtctccag
     1201 taagccatca cgagcctgaa tcacctcaac ttagatattt gccacctcag actgttgatc
     1261 atatatccca agaacacgaa ggggaccttg acctccatac aaatgatgta gataaaagtc
     1321 ttcaggatga cacagagcct gaaaatggat ctgatatttc cagtgcagaa aatgaacaga
     1381 ctgaagctga tcaggcaact gcagctgaaa cattatctaa aaatgaagtg ttatatgacg
     1441 gagaaaacca tgattgtgag gaaaagccac aagacattgt acagaacatt gtagaagaaa
     1501 tggtgaacat tgttgttgga gatatgggag aagggactac tataaatgca agtgcagatg
     1561 gcaacattgg aactatagag gatggtagtg acagtgaaaa tattcaagca aatggaattc
     1621 caggaacacc aatttctgtt gcatatacac catccttacc tgatgataga ttgtcagtct
     1681 cttccaatga tactcaggaa tctggaaatt cttcaggacc ttcacctggt gctaagtttt
     1741 cccacatttt acaaaaggat gcctttctag tattcaggtc attgtgtaaa ctgtcaatga
     1801 aaccactgtc agatggacca ccagatccaa agtctcatga actacgatcc aagattcttt
     1861 cattgcagtt acttctatcc attctgcaga atgcaggacc tattttcagg acaaatgaga
     1921 tgtttattaa tgctattaag cagtatcttt gtgttgcact ctcaaaaaat ggagtctcat
     1981 ctgttccaga ggtttttgag ctttctcttt ctatatttct tactttgttg tcaaatttca
     2041 agacacatct gaagatgcaa attgaggtgt tctttaaaga aattttctta tacattttgg
     2101 aaacttctac cagctcattt gatcacaaat ggatggttat tcagacactg acgaggattt
     2161 gtgcagatgc tcagagtgta gtggatattt atgtaaacta tgactgtgac ttaaatgcag
     2221 ccaatatatt tgaaagacta gtaaatgatc tatcaaaaat tgctcaagga aggggcagtc
     2281 aagaacttgg tatgagtaat gttcaggaat tgagcctgag gaaaaaaggt ttagaatgct
     2341 tagtgtcgat tttgaagtgt atggttgaat ggagtaagga tcagtatgtg aatcccaact
     2401 cccagacaac tcttggtcag gaaaaaccct cagagcaaga gatgagtgaa atcaaacacc
     2461 ctgagacaat aaacagatac ggaagtttaa attccctgga gtcaacatca tcatcaggaa
     2521 taggcagcta cagtacacag atgtctggca ctgataatcc agaacaattt gaggtcctaa
     2581 agcaacaaaa agaaataata gaacaaggga tagatttatt taataagaaa ccaaagagag
     2641 gaatacagta cctccaagaa caagggatgc ttggcaccac acctgaagat attgcccaat
     2701 tcttacatca agaggaaaga ttagactcta ctcaagtggg tgagttcctg ggagataatg
     2761 ataaatttaa caaagaagtc atgtatgcat atgtggacca acatgacttt tcaggaaaag
     2821 acttcgtttc agcccttcgt atgtttctag aaggatttcg tcttccaggg gaagctcaga
     2881 aaatcgatcg attaatggaa aaatttgctg caagatacct agaatgcaac caaggacaaa
     2941 ctctctttgc tagtgcggat acagcttatg ttttggctta ttcaattatc atgttgacca
     3001 cagaccttca cagtccacag gtgaaaaata aaatgacaaa ggaacaatac attaagatga
     3061 atagaggtat caatgacagt aaagaccttc ctgaagagta tctatcagcc atctataatg
     3121 aaatagctgg gaaaaagata tcaatgaaag aaacaaaaga actaacaatc cctacaaaat
     3181 caagtaaaca gaatgtagcc agtgaaaaac aaagaagact tctgtataac ttagaaatgg
     3241 agcagatggc caagacagct aaagcactca tggaggccgt gagccatgtt caggcaccct
     3301 ttacaagtgc aacacatttg gagcatgtga ggcccatgtt taagttggct tggacgcctt
     3361 ttctggctgc attcagtgtg ggtctacaag attgtgatga tactgaagta gcctctcttt
     3421 gcctggaagg tataagatgt gcaatcagaa ttgcatgcat tttcagcatt cagctggaga
     3481 gagatgcata tgtccaggca ctagcaagat ttaccttact cacagtgagt tccggtatta
     3541 ctgaaatgaa acagaagaac attgacacaa taaaaacact tatcacagtg gctcatacag
     3601 atggaaatta tttaggaaat tcatggcatg agattctgaa gtgcatcagt cagttggagc
     3661 tggcacagct tataggaact ggagtgaaac ctcgatacat ttctggaaca gtgcgaggca
     3721 gagaaggatc tcttactgga acaaaagatc aggctcctga tgaatttgtg ggtttagggc
     3781 tagttggagg aaatgtggac tggaaacaga tagcaagtat tcaggaatcc attggagaaa
     3841 ccagttctca gagtgttgtt gttgctgtag atagaatatt cacaggatct acaaggctag
     3901 atggaaatgc cattgtggat tttgtccgtt ggctctgtgc tgtgtctatg gatgaattac
     3961 tttccacgac acacccaaga atgtttagtc tacaaaaaat agtagaaata tcatattaca
     4021 acatgggaag aataagatta cagtggtctc gaatttggga agttattgga gatcatttta
     4081 ataaggttgg gtgtaatcct aatgaagatg tagctatttt tgcagtagac tccttgaggc
     4141 agttgtcaat gaagttctta gagaaagggg agcttgctaa cttcagattc cagaaggatt
     4201 tcttaagacc ttttgaacat ataatgaaac ggaacaggtc tccaacaatt cgagatatgg
     4261 ttgtacggtg tatagcacag atggttaatt ctcaagctgc taacattcga tctggatgga
     4321 agaacatttt ctctgtattt catctagctg catctgatca agatgaaagc atagtggaac
     4381 ttgcattcca aacaaccggg cacattgtca cccttgtatt tgaaaaacac tttccagcga
     4441 ccattgattc tttccaggat gcagtgaagt gtttgtctga atttgcgtgc aatgcagctt
     4501 tcccagacac aagtatggaa gcaattcgac ttattcgcca ttgtgcaaaa tatgtgtctg
     4561 atagacctca ggctttcaag gaatacacaa gcgatgatat gaacgtagca cctgaagaca
     4621 gggtgtgggt gagaggatgg ttcccaattc tctttgagtt atcctgtatc atcaatagat
     4681 gcaaattaga tgtaagaacc aggggtttaa cagtaatgtt tgaaataatg aaaacatatg
     4741 gccacactta tgagaaacac tggtggcagg atttatttag aattgttttc agaatctttg
     4801 acaatatgaa attgccagaa caacagacag agaaagctga atggatgaca acaacttgca
     4861 atcatgcact ttatgcaatc tgtgatgtat tcactcagta tttagaagta ctcagtgatg
     4921 tacttttgga tgacattttt gctcagctct actggtgtgt gcagcaagac aatgagcagt
     4981 tagcgcgatc tggtacaaac tgtttagaga atgttgttat tctgaatggt gaaaaattta
     5041 ccctagaaat ctgggataaa acttgcaact gcacactgga tatcttcaaa accacaatcc
     5101 cacatgcgct gttgacctgg cgacccaatt ctggagaaac tgccccccca cctccatctc
     5161 ctgtaagtga aaagccattg gatacaatat cacagaagtc tgtagatatt catgattcta
     5221 ttcaaccaag gtctgtggat aacagaccac aagcaccact ggtttctgcg tctgctgtta
     5281 atgaagaagt cagcaaaatt aaatctacag caaaatttcc agaacaaaaa ttgtttgctg
     5341 ccctgttgat taaatgtgtt gtgcagctgg aactcatcca gactatcgac aacattgtct
     5401 tcttcccagc cacaagtaag aaagaagatg cagaaaactt agctgcagca cagagagatg
     5461 cggtggactt tgatgttcgc gttgatactc aagaccaagg aatgtaccgc tttttaacat
     5521 cacaacaact ttttaagcta ctggactgct tattagagtc acatagattt gcaaaagcgt
     5581 ttaattccaa caacgaacag aggactgccc tgtggaaagc aggattcaaa ggcaaatcca
     5641 agcccaacct tctgaagcag gagaccagca gcctggcctg tgggctgcgc attctcttcc
     5701 ggatgtacat ggatgagagc cgcgttagtg cctgggagga ggtccagcag aggcttttga
     5761 atgtctgcag tgaagcacta agttacttcc tcactctaac atcagaaagt catcgagaag
     5821 cctggactaa cttactgctt ttgtttctaa ctaaagttct aaagataagt gataataggt
     5881 ttaaagctca tgcatcattc tactaccctc tcttatgtga aattatgcaa tttgacttga
     5941 ttcctgaact tcgtgctgtt cttagaagat tttttctgcg aatcggagta gtttttcaga
     6001 tatcacaacc acctgaacag gaacttggaa taaacaagca atgatgggaa cttaatattt
     6061 ttgttggcat ttacatccct ctgctcttta aaaggacgct ggagctgagg tttcctacct
     6121 gaaaaatgat ttctctggat tgcagtgtct gagttactgg taaagatgct tagaagtctt
     6181 actcaaactt gcaacactcc agtccctttt agtgctggtg gattttgtgt gttatattgg
     6241 cctcatgttg agcagaaagc ctgtttaaac agtgtcagct catgctcacg ggtccttccc
     6301 tgtcttccac ggcaggaaaa gccccacgtt tttggcaggt gtggaagtga aacttaccca
     6361 aagaactata gatgtaaaga ttgaacttct acaagaagta caactcagga gagctgtatt
     6421 ttgaaggata aaatgtttat aattggggag tggggagaga agaagaaatt attgttcatg
     6481 gtaaaagata tttagcaact atggtattct tattctgaag atttttgcac actcaggcta
     6541 tctgagatac tggtaatcat cctgtgaaaa atgtacagag atgcaggtct gtaatataaa
     6601 aatcttaaaa cattatatag ttcttcctgc actgttttct ttattttctt attcatttgc
     6661 taaataccca taatattttg tcaaatgcac taaacatttg ggtggaactt tcttttttat
     6721 tttataggga tttttagttt tgcccttttt ggtaggtggt gattttgagg ctgtaacatg
     6781 cccagaagct gttgtggccg acacttcaac aatagggaaa aaaaggtaga aaatatccct
     6841 actgacagta actacctgtc acatatttct cttaggactt ttaaagatga gccattaaaa
     6901 tagaatgatc ctttatggac caaaacttga atcactgcaa aatgaatcca gattgctgtc
     6961 attttctttt cttttgggtg gtggggcttg atgtagattt tactctatgt acagaattta
     7021 acgttgaata tattaaaata acaaatctgg catggtttgc ggaggttaga tttactggaa
     7081 atgtattcat actgtgaatt gtgctctgat ggttaaaaga caagattgtc aagcattccg
     7141 tattaacagt ggatgtagaa aattttttca gatggacaaa atgtatatgg tacagatgta
     7201 aagttttcta tgtaaaaaat tctgtacaac tttctgtaca atattgattc ccatctggca
     7261 tattctaatc aggttatagg tcaataaagt ttttgaatta tttcatcagt gcaatcaaaa
//
