LOCUS       NM_015230               7513 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens ArfGAP with RhoGAP domain, ankyrin repeat and PH
            domain 2 (ARAP2), transcript variant 1, mRNA.
ACCESSION   NM_015230
VERSION     NM_015230.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Xu,P., Wang,L., Liu,Q., Gao,P., Hu,F., Xie,X., Jiang,L., Bi,R.,
            Ding,F., Yang,Q. and Xiao,H.
  TITLE     The abnormal expression of circ-ARAP2 promotes ESCC progression
            through regulating miR-761/FOXM1 axis-mediated stemness and the
            endothelial-mesenchymal transition
  JOURNAL   J Transl Med 20 (1), 318 (2022)
   PUBMED   35842667
  REMARK    GeneRIF: The abnormal expression of circ-ARAP2 promotes ESCC
            progression through regulating miR-761/FOXM1 axis-mediated stemness
            and the endothelial-mesenchymal transition.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7513)
  AUTHORS   Luo,R., Chen,P.W., Kuo,J.C., Jenkins,L., Jian,X., Waterman,C.M. and
            Randazzo,P.A.
  TITLE     ARAP2 inhibits Akt independently of its effects on focal adhesions
  JOURNAL   Biol Cell 110 (12), 257-270 (2018)
   PUBMED   30144359
  REMARK    GeneRIF: These results highlight an Arf GAP-independent function of
            ARAP2 in regulating Akt activity.
REFERENCE   3  (bases 1 to 7513)
  AUTHORS   Chen,P.W., Luo,R., Jian,X. and Randazzo,P.A.
  TITLE     The Arf6 GTPase-activating proteins ARAP2 and ACAP1 define distinct
            endosomal compartments that regulate integrin alpha5beta1 traffic
  JOURNAL   J Biol Chem 289 (44), 30237-30248 (2014)
   PUBMED   25225293
  REMARK    GeneRIF: ACAP1 and ARAP2 each colocalize with Arf6 but they did not
            colocalize with each other and have opposing effects on focal
            adhesions.
REFERENCE   4  (bases 1 to 7513)
  AUTHORS   Cai,D.C., Fonteijn,H., Guadalupe,T., Zwiers,M., Wittfeld,K.,
            Teumer,A., Hoogman,M., Arias-Vasquez,A., Yang,Y., Buitelaar,J.,
            Fernandez,G., Brunner,H.G., van Bokhoven,H., Franke,B.,
            Hegenscheid,K., Homuth,G., Fisher,S.E., Grabe,H.J., Francks,C. and
            Hagoort,P.
  TITLE     A genome-wide search for quantitative trait loci affecting the
            cortical surface area and thickness of Heschl's gyrus
  JOURNAL   Genes Brain Behav 13 (7), 675-685 (2014)
   PUBMED   25130324
REFERENCE   5  (bases 1 to 7513)
  AUTHORS   Low,S.K., Chung,S., Takahashi,A., Zembutsu,H., Mushiroda,T.,
            Kubo,M. and Nakamura,Y.
  TITLE     Genome-wide association study of chemotherapeutic agent-induced
            severe neutropenia/leucopenia for patients in Biobank Japan
  JOURNAL   Cancer Sci 104 (8), 1074-1082 (2013)
   PUBMED   23648065
REFERENCE   6  (bases 1 to 7513)
  AUTHORS   Gavicherla,B., Ritchey,L., Gianfelice,A., Kolokoltsov,A.A.,
            Davey,R.A. and Ireton,K.
  TITLE     Critical role for the host GTPase-activating protein ARAP2 in
            InlB-mediated entry of Listeria monocytogenes
  JOURNAL   Infect Immun 78 (11), 4532-4541 (2010)
   PUBMED   20823205
  REMARK    GeneRIF: Our findings indicate that ARAP2 promotes InlB-mediated
            entry of Listeria monocytogenes in part, by antagonizing the host
            GTPase Arf6.
REFERENCE   7  (bases 1 to 7513)
  AUTHORS   Liu,C., Batliwalla,F., Li,W., Lee,A., Roubenoff,R., Beckman,E.,
            Khalili,H., Damle,A., Kern,M., Furie,R., Dupuis,J., Plenge,R.M.,
            Coenen,M.J., Behrens,T.W., Carulli,J.P. and Gregersen,P.K.
  TITLE     Genome-wide association scan identifies candidate polymorphisms
            associated with differential response to anti-TNF treatment in
            rheumatoid arthritis
  JOURNAL   Mol Med 14 (9-10), 575-581 (2008)
   PUBMED   18615156
REFERENCE   8  (bases 1 to 7513)
  AUTHORS   Yoon,H.Y., Miura,K., Cuthbert,E.J., Davis,K.K., Ahvazi,B.,
            Casanova,J.E. and Randazzo,P.A.
  TITLE     ARAP2 effects on the actin cytoskeleton are dependent on
            Arf6-specific GTPase-activating-protein activity and binding to
            RhoA-GTP
  JOURNAL   J Cell Sci 119 (Pt 22), 4650-4666 (2006)
   PUBMED   17077126
  REMARK    GeneRIF: ARAP2 is an Arf6GAP that functions downstream of RhoA to
            regulate focal adhesion dynamics.
REFERENCE   9  (bases 1 to 7513)
  AUTHORS   Miura,K., Jacques,K.M., Stauffer,S., Kubosaki,A., Zhu,K.,
            Hirsch,D.S., Resau,J., Zheng,Y. and Randazzo,P.A.
  TITLE     ARAP1: a point of convergence for Arf and Rho signaling
  JOURNAL   Mol Cell 9 (1), 109-119 (2002)
   PUBMED   11804590
REFERENCE   10 (bases 1 to 7513)
  AUTHORS   Krugmann,S., Anderson,K.E., Ridley,S.H., Risso,N., McGregor,A.,
            Coadwell,J., Davidson,K., Eguinoa,A., Ellson,C.D., Lipp,P.,
            Manifava,M., Ktistakis,N., Painter,G., Thuring,J.W., Cooper,M.A.,
            Lim,Z.Y., Holmes,A.B., Dove,S.K., Michell,R.H., Grewal,A.,
            Nazarian,A., Erdjument-Bromage,H., Tempst,P., Stephens,L.R. and
            Hawkins,P.T.
  TITLE     Identification of ARAP3, a novel PI3K effector regulating both Arf
            and Rho GTPases, by selective capture on phosphoinositide affinity
            matrices
  JOURNAL   Mol Cell 9 (1), 95-108 (2002)
   PUBMED   11804589
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC104078.3, AY049733.1,
            AC098827.3 and BX104334.1.
            
            On Nov 22, 2018 this sequence version replaced NM_015230.3.
            
            Summary: The protein encoded by this gene contains ARF-GAP,
            RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology
            domains. The protein is a phosphatidylinositol
            (3,4,5)-trisphosphate-dependent Arf6 GAP that binds RhoA-GTP, but
            it lacks the predicted catalytic arginine in the RHO-GAP domain and
            does not have RHO-GAP activity. The protein associates with focal
            adhesions and functions downstream of RhoA to regulate focal
            adhesion dynamics. [provided by RefSeq, Sep 2008].
            
            Transcript Variant: This variant (1) represents the protein-coding
            transcript and encodes the supported protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY049733.1, SRR18074967.813857.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000303965.9/ ENSP00000302895.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-242               AC104078.3         27053-27294         c
            243-5062            AY049733.1         1-4820
            5063-5103           AC098827.3         190813-190853       c
            5104-6852           AY049733.1         4862-6610
            6853-7513           BX104334.1         14-674              c
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p14"
     gene            1..7513
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /note="ArfGAP with RhoGAP domain, ankyrin repeat and PH
                     domain 2"
                     /db_xref="GeneID:116984"
                     /db_xref="HGNC:HGNC:16924"
                     /db_xref="MIM:606645"
     exon            1..336
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            337..1400
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     CDS             496..5610
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /note="Arf and Rho GAP adapter protein 2;
                     centaurin-delta-1"
                     /codon_start=1
                     /product="arf-GAP with Rho-GAP domain, ANK repeat and PH
                     domain-containing protein 2"
                     /protein_id="NP_056045.2"
                     /db_xref="CCDS:CCDS3441.1"
                     /db_xref="GeneID:116984"
                     /db_xref="HGNC:HGNC:16924"
                     /db_xref="MIM:606645"
                     /translation="MSSVSEVNVDIKDFLMSINLEQYLLHFHESGFTTVKDCAAINDS
                     LLQKIGISPTGHRRRILKQLQIILSKMQDIPIYANVHKTKKNDDPSKDYHVPSSDQNI
                     CIELSNSGSVQTSSPPQLETVRKNLEDSDASVERSQYPQSDDKLSPPKRDFPTAEEPH
                     LNLGSLNDSLFGSDNIKIESLITKKTVDHTVEEQQTEKVKLITENLSKLPNADSECLS
                     FVGCSTSGTNSGNGTNGLLEGSPPSPFFKFQGEMIVNDLYVPSSPILAPVRSRSKLVS
                     RPSRSFLLRHRPVPEIPGSTKGVSGSYFRERRNVATSTEKSVAWQNSNEENSSSIFPY
                     GETFLFQRLENSKKRSIKNEFLTQGEALKGEAATATNSFIIKSSIYDNRKEKISEDKV
                     EDIWIPREDKNNFLIDTASESEYSTVEECFQSLRRKNSKASKSRTQKALILDSVNRHS
                     YPLSSTSGNADSSAVSSQAISPYACFYGASAKKVKSGWLDKLSPQGKRMFQKRWVKFD
                     GLSISYYNNEKEMYSKGIIPLSAISTVRVQGDNKFEVVTTQRTFVFRVEKEEERNDWI
                     SILLNALKSQSLTSQSQAVVTPEKCGYLELRGYKAKIFTVLSGNSVWLCKNEQDFKSG
                     LGITIIPMNVANVKQVDRTVKQSFEIITPYRSFSFTAETEKEKQDWIEAVQQSIAETL
                     SDYEVAEKIWFNESNRSCADCKAPDPDWASINLCVVICKKCAGQHRSLGPKDSKVRSL
                     KMDASIWSNELIELFIVIGNKRANDFWAGNLQKDEELHMDSPVEKRKNFITQKYKEGK
                     FRKTLLASLTKEELNKALCAAVVKPDVLETMALLFSGADVMCATGDPVHSTPYLLAKK
                     AGQSLQMEFLYHNKFSDFPQHDIHSEGVLSQESSQSTFLCDFLYQAPSAASKLSSEKK
                     LLEETNKKWCVLEGGFLSYYENDKSTTPNGTININEVICLAIHKEDFYLNTGPIFIFE
                     IYLPSERVFLFGAETSQAQRKWTEAIAKHFVPLFAENLTEADYDLIGQLFYKDCHALD
                     QWRKGWFAMDKSSLHFCLQMQEVQGDRMHLRRLQELTISTMVQNGEKLDVLLLVEKGR
                     TLYIHGHTKLDFTVWHTAIEKAAGTDGNALQDQQLSKNDVPIIVNSCIAFVTQYGLGC
                     KYIYQKNGDPLHISELLESFKKDARSFKLRAGKHQLEDVTAVLKSFLSDIDDALLTKE
                     LYPYWISALDTQDDKERIKKYGAFIRSLPGVNRATLAAIIEHLYRVQKCSEINHMNAH
                     NLALVFSSCLFQTKGQTSEEVNVIEDLINNYVEIFEVKEDQVKQMDIENSFITKWKDT
                     QVSQAGDLLIEVYVERKEPDCSIIIRISPVMEAEELTNDILAIKNIIPTKGDIWATFE
                     VIENEELERPLHYKENVLEQVLRWSSLAEPGSAYLVVKRFLTADTIKHCSDRSTLGSI
                     KEGILKIKEEPSKILSGNKFQDRYFVLRDGFLFLYKDVKSSKHDKMFSLSSMKFYRGV
                     KKKMKPPTSWGLTAYSEKHHWHLCCDSSRTQTEWMTSIFIAQHEYDIWPPAGKERKRS
                     ITKNPKIGGLPLIPIQHEGNATLARKNIESARAELERLRLSEKCDKESVDSSLKERAS
                     MVAHCLEHKDDKLRNRPRKHRSFNCLEDTEPEAPLGQPKGHKGLKTLRKTEDRNSKAT
                     LDSDHKLPSRVIEELNVVLQRSRTLPKELQDEQILK"
     exon            1401..1459
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1536
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1628
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1982
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2052
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2173
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            2174..2352
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            2353..2468
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            2469..2668
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            2669..2754
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            2755..2937
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            2938..3112
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            3113..3247
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            3248..3392
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            3393..3495
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            3496..3694
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            3695..3758
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            3759..3922
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            3923..4135
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            4136..4241
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            4242..4389
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            4390..4458
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            4459..4533
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            4534..4651
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            4652..4780
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            4781..4920
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            4921..5003
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            5004..5039
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            5040..5103
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            5104..5238
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     exon            5239..7513
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7491..7496
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /note="hexamer: ATTAAA"
     polyA_site      7513
                     /gene="ARAP2"
                     /gene_synonym="CENTD1; PARX"
                     /note="major polyA site"
ORIGIN      
        1 gaggaagcgg cggccgaggc gaggcgcggg agcacctgag caaggcgcgg gcgccgggct
       61 cggcgtttcc ggagctgagc gcgggtccgc ggcccgcgct ccccgcctcc aggtgcggcg
      121 gccgcgacac gccggcccca ctaacagcgc cgcccgcgcg gccgccgcca ggcagcagag
      181 gcgactgcgg acagccggcg gggaccggga aagaggaggc ctggcgcctc cggcagaggg
      241 gcgaggaccg agcgcccaag gctgggagag acgccggcgc tgagcttccc agcgccgcct
      301 cacctcgctt ttcgaactcc gcgacccgag cgcaaggctt aagcaggtaa agcagtcact
      361 gtgaagaaaa taacatttta agaaactttt ctgtcacaat tgatagagga aaagccagta
      421 gagaagtaca tcttcttgtg tgtgtgtctc atcgtgccac tttttgtaac tcagcttagt
      481 aatgaagcca ccattatgtc ctcagtcagt gaagtaaatg tggatataaa agatttccta
      541 atgagcatta atttggagca gtatctctta catttccatg agtctggttt tactactgtg
      601 aaggactgtg cagcaataaa tgacagcctg ctgcagaaaa ttggaatatc acctacaggt
      661 caccgtagga ggatacttaa acagttacag ataatcttgt caaaaatgca agatattcca
      721 atatatgcaa atgttcataa aactaagaag aatgatgacc cttcaaagga ttaccatgtt
      781 ccatcttctg atcagaatat ctgcatagaa ctttccaatt ctggtagtgt tcagacatct
      841 agcccaccgc agttggagac tgttagaaaa aatcttgaag acagtgatgc aagtgtggaa
      901 agaagccagt atcctcaatc agatgataag ctgtctcctc ctaaacgcga cttccccact
      961 gcagaggaac cacacctgaa tttgggttct ttgaatgatt ctttatttgg tagtgacaat
     1021 attaaaatag aatcattgat tacaaagaag actgtggatc acacagttga agaacaacaa
     1081 acagaaaaag ttaaattgat cacagaaaat ctcagtaagc tccctaatgc agactctgaa
     1141 tgcctttctt ttgttggctg ttcaacatca ggaacaaatt ctggaaatgg aacaaatggt
     1201 ttattagaag gatcaccacc atccccattc tttaagtttc aaggagaaat gattgtaaat
     1261 gacttgtatg ttccatcatc accaatccta gcacctgtga gaagtcgtag caagttggtt
     1321 tcaagaccat ctcgatcttt tctgctaaga catcgacctg taccagagat tccagggtca
     1381 acaaaaggag tttctgggag ctatttccgt gaaagaagaa atgttgctac ctcaactgaa
     1441 aaatctgtgg catggcaaaa ttcaaatgag gagaattcat cttccatctt tccttatgga
     1501 gagacctttc tcttccagag actagaaaat tccaagaagc gatctataaa gaatgaattt
     1561 ttgacccagg gagaagcact caaaggggaa gcagctactg caacaaactc ttttatcatc
     1621 aaatcaagca tatacgataa cagaaaggag aaaataagcg aggacaaggt ggaagatatt
     1681 tggatacctc gagaggacaa aaacaatttt ttgatagaca ctgcttctga atcagaatac
     1741 tcaacagtag aagaatgctt tcagagttta agaagaaaaa attcaaaggc atctaaatct
     1801 aggactcaaa aagccttgat tttggactcc gttaataggc acagttatcc gttaagctca
     1861 acaagtggaa atgctgattc atcagccgtt tcttcacagg caatatctcc ctatgcctgc
     1921 ttttatggag catctgcaaa gaaggttaaa tcaggatggc tggataaact ctctcctcaa
     1981 ggaaaacgca tgtttcaaaa gagatgggtg aaatttgatg gccttagcat ttcttactac
     2041 aataatgaga aggagatgta ttcgaaagga ataattcccc tttctgctat atcaacagta
     2101 cgagttcaag gagacaacaa atttgaagtt gttacaacac aaagaacttt tgtttttaga
     2161 gtagaaaaag aagaggagag aaatgactgg atcagcatac tattaaatgc actgaaatca
     2221 caatccctta cctcgcagtc tcaagctgtt gttacacctg agaaatgtgg atatcttgaa
     2281 ttgagaggct ataaggcaaa aatttttact gtgttaagtg gaaacagtgt gtggctttgc
     2341 aaaaacgaac aggattttaa gagtggactt ggtattacca taattcctat gaatgtagca
     2401 aatgtaaagc aagtggaccg aactgtgaaa caatcttttg aaataatcac tccctacagg
     2461 agtttcagct ttacagccga gactgaaaag gagaaacaag actggattga agctgtgcag
     2521 caatcaatag cagaaactct ctctgattat gaagtagctg agaagatttg gttcaatgaa
     2581 tccaacagga gctgtgcaga ttgtaaagcc ccagatcctg actgggcatc catcaatctc
     2641 tgtgttgtca tctgtaagaa gtgtgcagga cagcatagat ctttaggacc aaaagattcc
     2701 aaggttagaa gtctaaaaat ggatgctagc atttggagca atgaactcat cgagcttttt
     2761 attgtcattg gaaacaaaag agcaaatgac ttttgggctg gtaatcttca aaaggatgaa
     2821 gaattacata tggactcacc agtagaaaag agaaaaaact ttattactca gaaatataaa
     2881 gaaggaaaat tcagaaaaac tcttttggca tctctcacca aagaagaatt aaataaggct
     2941 ctatgtgctg ctgtagtgaa accggatgtt ctagaaacaa tggctttgct gttcagtgga
     3001 gcagatgtca tgtgtgccac cggagacccc gtgcatagca ccccctatct gctagccaag
     3061 aaagctgggc aaagtctgca aatggaattt ctctaccata acaaattctc agatttccct
     3121 caacatgata ttcattccga gggtgtatta agtcaagagt cttcccagtc cacattcctc
     3181 tgtgactttt tatatcaagc tccttctgct gcttctaaac tctcttcaga gaaaaaactg
     3241 cttgaagaga caaataaaaa atggtgtgtt ttggaaggag gcttcttgag ttactatgaa
     3301 aatgataagt ctaccacacc taatggcacc attaatatca atgaagttat ctgcctggct
     3361 atacacaaag aggacttcta tttaaatact gggcccatct ttatctttga gatctactta
     3421 ccctccgaac gtgtgttttt atttggagct gaaacatctc aagctcaaag aaaatggaca
     3481 gaggcaatag ccaagcattt tgttccctta tttgctgaaa acttaacaga agctgactat
     3541 gatttgattg gtcaactctt ctacaaagac tgccatgccc tggatcagtg gagaaaaggc
     3601 tggtttgcta tggacaaatc cagcttgcat ttttgccttc aaatgcaaga agttcaggga
     3661 gatagaatgc acttaagaag actgcaagag ctaacaatca gcacaatggt tcaaaatggg
     3721 gaaaaactgg atgttttact cttggtagaa aaagggagaa cattatacat ccatgggcat
     3781 accaagttgg atttcacagt ctggcatact gcaattgaaa aagcagcagg tacagatggt
     3841 aatgctttac aagatcagca gctcagcaaa aatgacgttc ccattatagt gaacagctgt
     3901 atagcatttg ttacacagta tggtttagga tgcaaatata tctatcaaaa gaatggtgat
     3961 cctttgcata taagtgaact cctggagagt ttcaaaaagg atgcaagaag ctttaaattg
     4021 agggctggaa aacatcagct tgaagatgtg acggctgtgt tgaaaagttt tctctctgac
     4081 attgatgatg cactgcttac taaggagctc tacccatatt ggatctctgc tttagatacg
     4141 caagatgaca aggaaagaat taaaaaatat ggagcattta tacgttctct tccaggggtc
     4201 aaccgagcaa cactagcagc tatcattgaa cacctgtata gggttcagaa atgctcagaa
     4261 atcaatcaca tgaatgccca taatttggcc ttggtctttt catcctgttt gtttcaaacg
     4321 aagggacaaa ctagtgaaga agtgaatgta attgaggacc taattaataa ttatgtagaa
     4381 atatttgagg ttaaagaaga tcaagtcaaa caaatggaca tagaaaatag ctttattacc
     4441 aagtggaaag acacccaagt ttcccaggct ggagatttgt taattgaagt atatgtagaa
     4501 aggaaggaac ccgactgtag tattataatt cggatatctc ctgtgatgga agcagaagaa
     4561 ttaactaatg atatattagc gataaaaaat attattccta caaaaggtga tatttgggcc
     4621 acatttgaag tcattgaaaa tgaagagcta gagcgtcctc ttcactacaa ggaaaatgta
     4681 ctggagcagg tgcttcggtg gagttcatta gctgaacctg gctctgctta cctggtggtg
     4741 aagagattct taaccgctga cacaattaaa cactgcagtg accggagtac actgggaagc
     4801 atcaaagaag gaatcttgaa aatcaaagaa gaaccatcca aaatactatc tggaaataag
     4861 tttcaagacc ggtattttgt tttacgagat gggtttctct ttctttacaa ggatgtgaag
     4921 agtagtaaac atgacaagat gttttctctc agttccatga agttttatcg tggagtgaaa
     4981 aagaaaatga agcctccaac aagctgggga ttgaccgcat attctgagaa acatcactgg
     5041 cacctgtgtt gtgatagttc acgaactcag acggagtgga tgaccagtat ctttattgcc
     5101 cagcatgaat atgatatatg gccaccagct ggaaaggaac gaaaacgttc aataaccaaa
     5161 aatcccaaaa ttggaggttt gcctctgatt cctatacagc atgaggggaa tgcaaccttg
     5221 gcccggaaaa atattgagag tgcaagagca gaacttgaaa ggctgcggct cagtgaaaag
     5281 tgtgataaag agtccgtgga ctctagctta aaggagagag cttccatggt ggcccactgc
     5341 ctggagcaca aggacgataa acttcgaaat cgaccccgaa aacatcggag tttcaactgc
     5401 ctggaggaca cagagcctga agccccactt gggcaaccaa aaggccataa aggcctaaag
     5461 acattgagga agactgagga caggaatagc aaagctactt tggactctga tcataagtta
     5521 ccatcaaggg taattgaaga acttaatgtg gttctacaac ggtcaagaac ccttccaaaa
     5581 gaattacagg atgagcagat tttgaagtag gaaataaaat gaattgctcc ccagattatt
     5641 tttttaatat tgtatgcaat ctttatgtgt aactccaaaa ctgaactata agataattca
     5701 taagaatttg cctattgata ggcaaatttg tctatgctta ggcatttaag gagcatttaa
     5761 aaatatgtat atatgtgact gaatgtaaga ttaactttat tttggtctga acaaaccttg
     5821 taaagttcca ttgtattaac gtgtgtaaga agacattggg ttacttaggt ggtcagtcct
     5881 tttaaagtag ttgtctgtta agtggtataa tttatgagca gaaatctcaa actgtactgt
     5941 attgtataaa atgctgtgtt taaatgaagc ctaagaaact atctgtaaca tattttgcac
     6001 tttgagaaac cttgtatatt aaattaccat atgtttttag gtttacagaa gttctacctt
     6061 agggaaaaga aagggaagta aattgaaaaa ggggggagaa tttattgtag tggctgccta
     6121 aaaaccactg gagtttttaa aagaagcaag gagttaaatg tcctgcaaaa caaggaagtg
     6181 agctaagatt cattctagtt cactaatgac cagaacttgt caatgagaaa ccacttgtta
     6241 agaaaaagtc cagtgtttat ttctcaagag taatattcgg ccaacagatg taaataatag
     6301 cactagccac tcttattaat atttatagtg ttagaaaacc agccacatta cagattcact
     6361 acgtttgagc tcattgttgg tggctgctaa gaggaaactt acactgcatt ggagaggttg
     6421 tgtacacagg acctggcctg gatgctgatt gcactgtcat tgtatgcaag cattttgtct
     6481 tttttcagaa tttctgacta tacttttggt acgaacaaaa ttcatcacca cagggtcctg
     6541 ttgttttgtt tgatttttag atttttttaa attattatta aggttgtaat gtttttctcc
     6601 gagttgtcag aaataactgg aagaatacaa tatatggcca ttatggagcc aggggctagt
     6661 tggtggttta ctatacattt gggggctacc tggtcacatt taatctaggt ttttttttct
     6721 ttttttaagt aaaggtgtgg tctcttccct gtacttatga ataaggacaa agccctattt
     6781 ttgtttccac ataaagatca gctactgaat tggtgaattt cctgacagac aatgctatct
     6841 aaccatagtt catgttaggg gaagcatttt tccatcagtt tctttaaaat tggattgtct
     6901 ggtgagtttt gatattaaac attatgtagc taagtttttt ttttttagca tttctttttt
     6961 atttttcaat ctatattcac aggcccatac ttcagtcagt ccaatcatag tacagtgatg
     7021 tggtggtaga tgctatgaat ttcttcattg taaaatacgg atcatttgta ttttggggtg
     7081 ataaaatagt tcaccatggg tatgagatat ttattcttta aatcaaagta aattagaatt
     7141 tttaaaaagc acaaaactgc aggacagttt atgaaatagg tggcactatt agggaatctt
     7201 cctttaaagc aagaaatcat gttatttaga aagaaaaact aatcttaaac atactatttc
     7261 taataaatat ttatattttt atgaaataaa gaggtatgtg gaaattaata tttggtgatg
     7321 ttggacagtg gaaaagtatc tagagttttt acctgcctta tctgaattct tcttgaaact
     7381 tgagcttaaa ctctaatagc tgtttccctt tctattctga acaactgtct ccatttttca
     7441 agtgtgagag ataagggcta atgatgacat ctcatttatt ttgagtaaac attaaaattg
     7501 ttttacaagt cta
//
