LOCUS       NM_001654               2378 bp    mRNA    linear   PRI 05-DEC-2024
DEFINITION  Homo sapiens A-Raf proto-oncogene, serine/threonine kinase (ARAF),
            transcript variant 1, mRNA.
ACCESSION   NM_001654
VERSION     NM_001654.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Liu,R., Guo,Y., Han,L., Feng,S., Cao,J., Sun,Y., Cao,Z. and Cui,X.
  TITLE     Somatic ARAF mutations in pediatric Langerhans cell histiocytosis:
            clinicopathologic, genetic and functional profiling
  JOURNAL   Clin Exp Med 23 (8), 5269-5279 (2023)
   PUBMED   37572153
  REMARK    GeneRIF: Somatic ARAF mutations in pediatric Langerhans cell
            histiocytosis: clinicopathologic, genetic and functional profiling.
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   3  (bases 1 to 2378)
  AUTHORS   Su,W., Mukherjee,R., Yaeger,R., Son,J., Xu,J., Na,N., Merna
            Timaul,N., Hechtman,J., Paroder,V., Lin,M., Mattar,M., Qiu,J.,
            Chang,Q., Zhao,H., Zhang,J., Little,M., Adachi,Y., Han,S.W.,
            Taylor,B.S., Ebi,H., Abdel-Wahab,O., de Stanchina,E., Rudin,C.M.,
            Janne,P.A., McCormick,F., Yao,Z. and Rosen,N.
  TITLE     ARAF protein kinase activates RAS by antagonizing its binding to
            RASGAP NF1
  JOURNAL   Mol Cell 82 (13), 2443-2457 (2022)
   PUBMED   35613620
  REMARK    GeneRIF: ARAF protein kinase activates RAS by antagonizing its
            binding to RASGAP NF1.
REFERENCE   4  (bases 1 to 2378)
  AUTHORS   Mooz,J., Riegel,K., Ps,H., Sadanandam,A., Marini,F., Klein,M.,
            Werner,U., Roth,W., Wilken-Schmitz,A., Tegeder,I. and Rajalingam,K.
  TITLE     ARAF suppresses ERBB3 expression and metastasis in a subset of lung
            cancers
  JOURNAL   Sci Adv 8 (11), eabk1538 (2022)
   PUBMED   35302851
  REMARK    GeneRIF: ARAF suppresses ERBB3 expression and metastasis in a
            subset of lung cancers.
REFERENCE   5  (bases 1 to 2378)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 2378)
  AUTHORS   Papin,C., Eychene,A., Brunet,A., Pages,G., Pouyssegur,J.,
            Calothy,G. and Barnier,J.V.
  TITLE     B-Raf protein isoforms interact with and phosphorylate Mek-1 on
            serine residues 218 and 222
  JOURNAL   Oncogene 10 (8), 1647-1651 (1995)
   PUBMED   7731720
REFERENCE   7  (bases 1 to 2378)
  AUTHORS   Popescu,N.C. and Mark,G.E.
  TITLE     Localization of the pKs gene, a raf related sequence on human
            chromosomes X and 7
  JOURNAL   Oncogene 4 (4), 517-519 (1989)
   PUBMED   2717185
REFERENCE   8  (bases 1 to 2378)
  AUTHORS   Beck,T.W., Huleihel,M., Gunnell,M., Bonner,T.I. and Rapp,U.R.
  TITLE     The complete coding sequence of the human A-raf-1 oncogene and
            transforming activity of a human A-raf carrying retrovirus
  JOURNAL   Nucleic Acids Res 15 (2), 595-609 (1987)
   PUBMED   3029685
REFERENCE   9  (bases 1 to 2378)
  AUTHORS   Mark,G.E., Seeley,T.W., Shows,T.B. and Mountz,J.D.
  TITLE     Pks, a raf-related sequence in humans
  JOURNAL   Proc Natl Acad Sci U S A 83 (17), 6312-6316 (1986)
   PUBMED   3529082
REFERENCE   10 (bases 1 to 2378)
  AUTHORS   Belanger,B.F., Williams,W.J. and Yin,T.C.
  TITLE     A flexible renewal process simulator for neural spike trains
  JOURNAL   IEEE Trans Biomed Eng 23 (3), 262-266 (1976)
   PUBMED   1262038
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC362427.1 and BC002466.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001654.4.
            
            Transcript Variant: This variant (1) encodes the predominant
            isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.62376.1,
                                           SRR1803617.204242.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000377045.9/ ENSP00000366244.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                DC362427.1         106-120
            16-2378             BC002466.2         1-2363
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp11.3"
     gene            1..2378
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /note="A-Raf proto-oncogene, serine/threonine kinase"
                     /db_xref="GeneID:369"
                     /db_xref="HGNC:HGNC:646"
                     /db_xref="MIM:311010"
     exon            1..47
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     exon            48..202
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     CDS             107..1927
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Oncogene ARAF1; v-raf murine sarcoma 3611 viral oncogene
                     homolog 1; A-Raf proto-oncogene serine/threonine-protein
                     kinase; Ras-binding protein DA-Raf; proto-oncogene Pks;
                     proto-oncogene A-Raf-1; v-raf murine sarcoma 3611 viral
                     oncogene-like protein"
                     /codon_start=1
                     /product="serine/threonine-protein kinase A-Raf isoform 1"
                     /protein_id="NP_001645.1"
                     /db_xref="CCDS:CCDS35232.1"
                     /db_xref="GeneID:369"
                     /db_xref="HGNC:HGNC:646"
                     /db_xref="MIM:311010"
                     /translation="MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDS
                     LDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFV
                     RKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRQQFYHSVQD
                     LSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNV
                     HMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPA
                     EQRERKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVA
                     VKVLKVSQPTAEQAQAFKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYH
                     HLHVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGL
                     ATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLP
                     YSHIGCRDQIIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLFPQILAT
                     IELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP"
     exon            203..306
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     exon            307..409
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     exon            410..564
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     exon            565..663
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     exon            664..805
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     exon            806..833
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     exon            834..979
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1182
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1183..1359
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1406
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1525
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1657
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..1792
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1793..2378
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2378
                     /gene="ARAF"
                     /gene_synonym="A-RAF; ARAF1; PKS2; RAFA1"
                     /note="major polyA site"
ORIGIN      
        1 aagagaggcc caagatggag acggcggcgg ctgtagcggc gtgacaggag ccccatggca
       61 cctgcccagc cccacctcag cccatcttga caaaatctaa ggctccatgg agccaccacg
      121 gggcccccct gccaatgggg ccgagccatc ccgggcagtg ggcaccgtca aagtatacct
      181 gcccaacaag caacgcacgg tggtgactgt ccgggatggc atgagtgtct acgactctct
      241 agacaaggcc ctgaaggtgc ggggtctaaa tcaggactgc tgtgtggtct accgactcat
      301 caagggacga aagacggtca ctgcctggga cacagccatt gctcccctgg atggcgagga
      361 gctcattgtc gaggtccttg aagatgtccc gctgaccatg cacaattttg tacggaagac
      421 cttcttcagc ctggcgttct gtgacttctg ccttaagttt ctgttccatg gcttccgttg
      481 ccaaacctgt ggctacaagt tccaccagca ttgttcctcc aaggtcccca cagtctgtgt
      541 tgacatgagt accaaccgcc aacagttcta ccacagtgtc caggatttgt ccggaggctc
      601 cagacagcat gaggctccct cgaaccgccc cctgaatgag ttgctaaccc cccagggtcc
      661 cagcccccgc acccagcact gtgacccgga gcacttcccc ttccctgccc cagccaatgc
      721 ccccctacag cgcatccgct ccacgtccac tcccaacgtc catatggtca gcaccacggc
      781 ccccatggac tccaacctca tccagctcac tggccagagt ttcagcactg atgctgccgg
      841 tagtagagga ggtagtgatg gaaccccccg ggggagcccc agcccagcca gcgtgtcctc
      901 ggggaggaag tccccacatt ccaagtcacc agcagagcag cgcgagcgga agtccttggc
      961 cgatgacaag aagaaagtga agaacctggg gtaccgggac tcaggctatt actgggaggt
     1021 accacccagt gaggtgcagc tgctgaagag gatcgggacg ggctcgtttg gcaccgtgtt
     1081 tcgagggcgg tggcatggcg atgtggccgt gaaggtgctc aaggtgtccc agcccacagc
     1141 tgagcaggcc caggctttca agaatgagat gcaggtgctc aggaagacgc gacatgtcaa
     1201 catcttgctg tttatgggct tcatgacccg gccgggattt gccatcatca cacagtggtg
     1261 tgagggctcc agcctctacc atcacctgca tgtggccgac acacgcttcg acatggtcca
     1321 gctcatcgac gtggcccggc agactgccca gggcatggac tacctccatg ccaagaacat
     1381 catccaccga gatctcaagt ctaacaacat cttcctacat gaggggctca cggtgaagat
     1441 cggtgacttt ggcttggcca cagtgaagac tcgatggagc ggggcccagc ccttggagca
     1501 gccctcagga tctgtgctgt ggatggcagc tgaggtgatc cgtatgcagg acccgaaccc
     1561 ctacagcttc cagtcagacg tctatgccta cggggttgtg ctctacgagc ttatgactgg
     1621 ctcactgcct tacagccaca ttggctgccg tgaccagatt atctttatgg tgggccgtgg
     1681 ctatctgtcc ccggacctca gcaaaatctc cagcaactgc cccaaggcca tgcggcgcct
     1741 gctgtctgac tgcctcaagt tccagcggga ggagcggccc ctcttccccc agatcctggc
     1801 cacaattgag ctgctgcaac ggtcactccc caagattgag cggagtgcct cggaaccctc
     1861 cttgcaccgc acccaggccg atgagttgcc tgcctgccta ctcagcgcag cccgccttgt
     1921 gccttaggcc ccgcccaagc caccagggag ccaatctcag ccctccacgc caaggagcct
     1981 tgcccaccag ccaatcaatg ttcgtctctg ccctgatgct gcctcaggat cccccattcc
     2041 ccaccctggg agatgagggg gtccccatgt gcttttccag ttcttctgga attgggggac
     2101 ccccgccaaa gactgagccc cctgtctcct ccatcatttg gtttcctctt ggctttgggg
     2161 atacttctaa attttgggag ctcctccatc tccaatggct gggatttgtg gcagggattc
     2221 cactcagaac ctctctggaa tttgtgcctg atgtgccttc cactggattt tggggttccc
     2281 agcaccccat gtggattttg gggggtccct tttgtgtctc ccccgccatt caaggactcc
     2341 tctctttctt caccaagaag cacagaattc tgctgggc
//
