LOCUS       NM_012096               6069 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens adaptor protein, phosphotyrosine interacting with PH
            domain and leucine zipper 1 (APPL1), mRNA.
ACCESSION   NM_012096
VERSION     NM_012096.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Du,Y., Duan,Y., Zhao,J., Liu,C., Zhang,Z., Zhang,J., Meng,Z.,
            Wang,X., Lau,W.B., Xie,D., Lopez,B.L., Christopher,T.A., Gao,E.,
            Koch,W.W., Liu,H., Liu,D., Ma,X.L., Gu,G. and Wang,Y.
  TITLE     Dysfunctional APPL1-Mediated Epigenetic Regulation in Diabetic
            Vascular Injury
  JOURNAL   Arterioscler Thromb Vasc Biol 43 (12), e491-e508 (2023)
   PUBMED   37795615
  REMARK    GeneRIF: Dysfunctional APPL1-Mediated Epigenetic Regulation in
            Diabetic Vascular Injury.
            Erratum:[Arterioscler Thromb Vasc Biol. 2024 May;44(5):e171. doi:
            10.1161/ATV.0000000000000171.. PMID: 38657036]
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Yuan,W., Liu,W., Zhang,Y., Wang,X., Xu,C., Li,Q., Ji,P., Wang,J.,
            Feng,P., Wu,Y., Shen,H. and Wang,P.
  TITLE     Reduced APPL1 impairs osteogenic differentiation of mesenchymal
            stem cells by facilitating MGP expression to disrupt the BMP2
            pathway in osteoporosis
  JOURNAL   J Biol Chem 299 (6), 104823 (2023)
   PUBMED   37187293
  REMARK    GeneRIF: Reduced APPL1 impairs osteogenic differentiation of
            mesenchymal stem cells by facilitating MGP expression to disrupt
            the BMP2 pathway in osteoporosis.
REFERENCE   3  (bases 1 to 6069)
  AUTHORS   Yang,M., Gong,C., Song,K., Huang,N., Chen,H., Gong,H., Yang,Y.,
            Guo,S. and Xiao,H.
  TITLE     APPL1 Is a Prognostic Biomarker and Correlated with Treg Cell
            Infiltration via Oxygen-Consuming Metabolism in Renal Clear Cell
            Carcinoma
  JOURNAL   Oxid Med Cell Longev 2023, 5885203 (2023)
   PUBMED   36846720
  REMARK    GeneRIF: APPL1 Is a Prognostic Biomarker and Correlated with Treg
            Cell Infiltration via Oxygen-Consuming Metabolism in Renal Clear
            Cell Carcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6069)
  AUTHORS   Martini,C., Logan,J.M., Sorvina,A., Gordon,C., Beck,A.R., S-Y
            Ung,B., Caruso,M.C., Moore,C., Hocking,A., Johnson,I.R.D., Li,K.L.,
            Karageorgos,L., Hopkins,A.M., Esterman,A.J., Huzzell,C.,
            Brooks,R.D., Lazniewska,J., Hickey,S.M., Bader,C.,
            Parkinson-Lawrence,E., Weigert,R., Sorich,M.J., Tewari,P.,
            Martin,C., O'Toole,S., Bates,M., Ward,M., Mohammed,B., Keegan,H.,
            Watson,W., Prendergast,S., Heffernan,S., NiMhaolcatha,S.,
            O'Connor,R., Malone,V., Carter,M., Ryan,K., Brady,N., Clarke,A.,
            Sokol,F., Prabhakaran,S., Stahl,J., Klebe,S., Samaratunga,H.,
            Delahunt,B., Selemidis,S., Moretti,K.L., Butler,L.M., O'Leary,J.J.
            and Brooks,D.A.
  TITLE     Aberrant protein expression of Appl1, Sortilin and Syndecan-1
            during the biological progression of prostate cancer
  JOURNAL   Pathology 55 (1), 40-51 (2023)
   PUBMED   36089417
  REMARK    GeneRIF: Aberrant protein expression of Appl1, Sortilin and
            Syndecan-1 during the biological progression of prostate cancer.
REFERENCE   5  (bases 1 to 6069)
  AUTHORS   Zhang,Y., Liu,W., Yuan,W., Cai,Z., Ye,G., Zheng,G., Xu,C., Wang,X.,
            Zeng,C., Mi,R., Feng,P., Chen,F., Wu,Y., Shen,H. and Wang,P.
  TITLE     Impairment of APPL1/Myoferlin facilitates adipogenic
            differentiation of mesenchymal stem cells by blocking autophagy
            flux in osteoporosis
  JOURNAL   Cell Mol Life Sci 79 (9), 488 (2022)
   PUBMED   35984564
  REMARK    GeneRIF: Impairment of APPL1/Myoferlin facilitates adipogenic
            differentiation of mesenchymal stem cells by blocking autophagy
            flux in osteoporosis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6069)
  AUTHORS   Miaczynska,M., Christoforidis,S., Giner,A., Shevchenko,A.,
            Uttenweiler-Joseph,S., Habermann,B., Wilm,M., Parton,R.G. and
            Zerial,M.
  TITLE     APPL proteins link Rab5 to nuclear signal transduction via an
            endosomal compartment
  JOURNAL   Cell 116 (3), 445-456 (2004)
   PUBMED   15016378
  REMARK    GeneRIF: identification of a pathway directly linking the small
            GTPase Rab5, a key regulator of endocytosis, to signal transduction
            and mitogenesis via APPL1 and APPL2, two Rab5 effectors
REFERENCE   7  (bases 1 to 6069)
  AUTHORS   Yang,L., Lin,H.K., Altuwaijri,S., Xie,S., Wang,L. and Chang,C.
  TITLE     APPL suppresses androgen receptor transactivation via potentiating
            Akt activity
  JOURNAL   J Biol Chem 278 (19), 16820-16827 (2003)
   PUBMED   12621049
REFERENCE   8  (bases 1 to 6069)
  AUTHORS   Liu,J., Yao,F., Wu,R., Morgan,M., Thorburn,A., Finley,R.L. Jr. and
            Chen,Y.Q.
  TITLE     Mediation of the DCC apoptotic signal by DIP13 alpha
  JOURNAL   J Biol Chem 277 (29), 26281-26285 (2002)
   PUBMED   12011067
REFERENCE   9  (bases 1 to 6069)
  AUTHORS   Mitsuuchi,Y., Johnson,S.W., Sonoda,G., Tanno,S., Golemis,E.A. and
            Testa,J.R.
  TITLE     Identification of a chromosome 3p14.3-21.1 gene, APPL, encoding an
            adaptor molecule that interacts with the
            oncoprotein-serine/threonine kinase AKT2
  JOURNAL   Oncogene 18 (35), 4891-4898 (1999)
   PUBMED   10490823
REFERENCE   10 (bases 1 to 6069)
  AUTHORS   Naylor,R., Knight Johnson,A. and del Gaudio,D.
  TITLE     Maturity-Onset Diabetes of the Young Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   29792621
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC093928.2, BC028599.2 and
            AF169797.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_012096.2.
            
            Summary: The protein encoded by this gene has been shown to be
            involved in the regulation of cell proliferation, and in the
            crosstalk between the adiponectin signalling and insulin signalling
            pathways. The encoded protein binds many other proteins, including
            RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of
            the NuRD/MeCP1 complex. This protein is found associated with
            endosomal membranes, but can be released by EGF and translocated to
            the nucleus. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.53167.1,
                                           SRR1660805.136161.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000288266.8/ ENSP00000288266.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 AC093928.2         57483-57490
            9-223               BC028599.2         1-215
            224-3812            AF169797.1         127-3715
            3813-6069           AC093928.2         100969-103225
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p14.3"
     gene            1..6069
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /note="adaptor protein, phosphotyrosine interacting with
                     PH domain and leucine zipper 1"
                     /db_xref="GeneID:26060"
                     /db_xref="HGNC:HGNC:24035"
                     /db_xref="MIM:604299"
     exon            1..209
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     CDS             156..2285
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /note="signaling adaptor protein DIP13alpha; adaptor
                     protein containing pH domain, PTB domain and leucine
                     zipper motif 1; AKT2 interactor; adapter protein
                     containing PH domain, PTB domain and leucine zipper motif
                     1; dip13-alpha; adaptor protein, phosphotyrosine
                     interaction, PH domain and leucine zipper containing 1"
                     /codon_start=1
                     /product="DCC-interacting protein 13-alpha"
                     /protein_id="NP_036228.1"
                     /db_xref="CCDS:CCDS2882.1"
                     /db_xref="GeneID:26060"
                     /db_xref="HGNC:HGNC:24035"
                     /db_xref="MIM:604299"
                     /translation="MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAM
                     HRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAV
                     LSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVK
                     YEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENL
                     NEQLEEFLANIGTSVQNVRREMDSDIETMQQTIEDLEVASDPLYVPDPDPTKFPVNRN
                     LTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVD
                     CEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQ
                     SALEAVTPSPSFQQRHESLRPAAGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPD
                     TPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLG
                     SMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTF
                     PLPCVVLYATHQENKRLFGFVLRTSSGRSESNLSSVCYIFESNNEGEKICDSVGLAKQ
                     IALHAELDRRASEKQKEIERVKEKQQKELNKQKQIEKDLEEQSRLIAASSRPNQASSE
                     GQFVVLSSSQSEESDLGEGGKKRESEA"
     exon            210..308
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            309..368
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            369..440
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            441..528
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            529..570
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            571..629
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            630..776
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            777..859
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            860..1018
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1207
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1250
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1307
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            1308..1402
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1585
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            1586..1638
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..1813
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..1850
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            1851..1997
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            1998..2048
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            2049..2138
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     exon            2139..6069
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2440
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /note="major polyA site"
     regulatory      3950..3955
                     /regulatory_class="polyA_signal_sequence"
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /note="hexamer: AGTAAA"
     polyA_site      3974
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /note="major polyA site"
     regulatory      6030..6035
                     /regulatory_class="polyA_signal_sequence"
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /note="hexamer: AATAAA"
     polyA_site      6069
                     /gene="APPL1"
                     /gene_synonym="APPL; DIP13alpha; MODY14"
                     /note="major polyA site"
ORIGIN      
        1 gcacggccgc tcggcgcctg gagaaggctg tgcgggcggg gacggctgca gcccttgccg
       61 gagagggcgg gccggggtca gctgcggcgg gcgggccggc gcggggagct gtgggcggca
      121 gctgcgtctc ctgccaccgc cctccctccg ccacgatgcc ggggatcgac aagctgccca
      181 tcgaggagac gctggaggac agcccgcaga caaggtcttt actaggtgta tttgaagaag
      241 atgccacagc tatttccaac tatatgaacc agttgtatca agctatgcat cggatttatg
      301 atgcacagaa tgaattaagt gcagcaacac acctgacctc aaaactttta aaagaatatg
      361 aaaaacagcg ttttccattg ggaggtgatg atgaagttat gagctctaca ttgcaacagt
      421 tttcaaaagt tatagatgag cttagctctt gtcatgcagt gctttcaact caacttgctg
      481 atgccatgat gttccccatt acccagttta aagaaagaga tctgaaagaa atactaacat
      541 taaaggaagt atttcagatt gcaagtaatg atcatgatgc tgcgattaat agatatagcc
      601 gtttatcaaa aaaaagagaa aatgacaagg tgaagtatga agtaacagaa gatgtgtaca
      661 catccagaaa gaaacaacac cagaccatga tgcattattt ttgtgcatta aatactcttc
      721 agtacaagaa gaaaatagca ttgttagaac ctctacttgg gtacatgcaa gctcagataa
      781 gtttctttaa gatgggttct gaaaatctta atgaacaact ggaagaattt ttagctaata
      841 ttggaacaag cgttcagaat gttcgcaggg aaatggacag tgatatagag accatgcaac
      901 agacaataga ggatttggaa gtagccagtg atcccttata tgtgcctgac ccagacccca
      961 ccaaatttcc tgttaatcga aatttaaccc gaaaggctgg ataccttaat gctaggaata
     1021 aaacaggctt ggtgtcatct acctgggaca gacagtttta cttcacgcag ggtggaaatt
     1081 taatgagtca ggcccgtggg gatgtagcag gaggcctggc catggacata gacaactgtt
     1141 cagtgatggc tgtggactgt gaagacagac gatattgttt tcagatcacc tctttcgatg
     1201 gaaaaaaatc ttcaattttg caagcagaga gtaaaaaaga tcatgaagag tggatctgta
     1261 caataaacaa catatctaaa caaatatact taagtgaaaa tccagaggaa actgctgcac
     1321 gagtaaatca atcagctctg gaagctgtca ctccttcccc atctttccag cagaggcacg
     1381 agagcctgcg gccagcagca ggacaatctc ggccaccgac agctcgaacc agcagttcag
     1441 gatccttagg atctgagtct acaaatttgg ctgccctctc tctagattct cttgttgccc
     1501 cagacacccc aatacagttt gacataattt ctcctgtgtg tgaagatcag cctggccagg
     1561 caaaagcctt tggccaggga ggcaggcgta caaatccatt tggagaatct ggaggaagta
     1621 caaaatctga aactgaagat tctattcttc atcagttatt tattgtccga ttccttggtt
     1681 caatggaggt gaaatcagat gaccatccag atgttgttta tgaaactatg cgccaaatct
     1741 tagctgcccg ggccatccat aacatctttc gtatgacaga atcgcattta ttagtcactt
     1801 gtgactgttt aaagttaatt gatccacaga cacaagttac aaggctcacg tttccattac
     1861 cttgtgtagt tttgtatgct acacaccagg aaaataagcg cctttttgga tttgttcttc
     1921 ggacatcaag cgggagaagt gaaagtaatc tgtcatcagt ctgctatata tttgagtcaa
     1981 acaatgaggg ggaaaagata tgtgattctg ttggactggc aaaacagata gctttgcatg
     2041 ctgaactgga tcgtagggca tcagaaaaac aaaaagaaat agagagagta aaagagaagc
     2101 aacagaaaga actcaataaa caaaaacaga ttgaaaagga cttggaagaa caaagtcggt
     2161 tgatagctgc ttccagtaga ccaaaccaag ccagtagtga ggggcagttt gttgtcctta
     2221 gcagtagcca gtcagaagag agtgatttgg gagaaggagg aaagaagaga gaatcagaag
     2281 cataagctta tacttttggt agatattccc ccttggaatt tgacagtttc tatggtgaaa
     2341 tggcagaagg taacaactat gttgaaatat caaggaggag attaagcttt atatttgctt
     2401 atttgttgta gctacatttt aaaaaaaaga ttgaacttga tgacttaggt ttgcattgat
     2461 cttttttccc ccttaaacat aatgtactat gtattaacat ctaaaggaaa cctgctcatc
     2521 tccctgaagc agactgctga ggaattacat ttgctcaaga attttttccg tcaaattgtg
     2581 aacttttaat tcttgcattg taattggctg tgtccataag aaatcattta ttcttcaggc
     2641 ttagacattc atggatatgc ttttctttca ttaggaacat tttgctggtg atgaggaagc
     2701 atttagctga ataagtttaa agccctttat acagagaatt ctacaagttt gcaaatattt
     2761 taacaatatt aaatgtgcaa tagaactttt ataaaataat tagaacagag attttacagt
     2821 taaggtttca aattgtggca ggtggtactg ttgatctcag ggtactttct gggattgctc
     2881 acatttctct aatgtactgc acttgatgcc agtaggaaag aagcttaagt gtcttcagtt
     2941 caagattgat agagcccttg gcattttatt atcacattct tagttctcag gttgggactt
     3001 caattactgc tgcagagcag tagtggttaa aaataagata ttggaattta ttaaaagatt
     3061 tttgttcaat acattttaga ttaggattga caagtaaaga tactgctatg gaatgataca
     3121 ttgtattttc tgcattgtgt gaaatagttt ttattgaaag tcaagtgaca tttcaaaaga
     3181 agttctataa caattatgtt tcatgcttaa agtaaaaatt cccagagttt agtttagaaa
     3241 atgtaatctt ttaaatttca gactgatata ttcccagtat tttcataatg ccatgttttg
     3301 ataaagtact gaatcaccac tttatatcca caaaggcaaa taactaatgt atgataatag
     3361 aataatttgc actaattatt gtaaatatgt ctactcttca aatgagtaaa aggtccaatt
     3421 ttgtgaaaga ttgaaaatga attgaatgcc tagtagagta taggagaagt tgaccaagga
     3481 aggatttaac ctggtgccaa ggttaaaata acatttctct actgcctatt gtttatgtta
     3541 aaccttaatt ttttttctgt aatcactttt aacactgcta atagaaatta tgttttcatg
     3601 tgtttcacat gaaaaagtat atatatacaa agttaatata gtgggtaaaa tactttacat
     3661 agtcacacat ttacaaattt ttcaagaggt tagccactaa gactttaata attttacaag
     3721 ggaaaaagcc tttttttttc tttgatatac agttttttct tcttagttct gcattagaaa
     3781 tggcatctgt tttaggtctc aaaatataac tcagctgttt cacactgtat atgtacattg
     3841 ttttctgtag gaataggata atgatatata ggatcatgat attccttcta tccatgtgcc
     3901 aaatgggtgt aatgtttatt tactgatgct ttatgttacc aaaacataca gtaaaaaagt
     3961 agaaatttat gaaatacttt tgataaaaag tttattttgt gcttaccaaa aggaatgctt
     4021 tcacaatagt gtatcagttc ttttgttttg ttaaagttgg aatttattct gttgccagca
     4081 tttaagtagt catggcaagt cctgttttta agaccttttg gagactggag ctttctgttc
     4141 cattaagtct tttgtttata ctacaaattg tcacctcact tagttcagat gaaatctgtt
     4201 actctacaag gaaggtgttc atcattagga ggcagcttta ctaagctggt gctttgcatg
     4261 gtagcaagtg ctgcccttta tcagcaccct gggtcatagt gtaggctaga gttaaggcac
     4321 tggcagactt agggatgctg gacagacctg tagttcgttt taagtcatgt tcacaggaat
     4381 ttctacaata ataaacccat catctccata ggtcagatcg aagtgcattc caatgctaaa
     4441 tagaagttat gagtgggttt aacaatttta gatgattcag cttttgttcc attactgttg
     4501 aactatatga actattccat tactgcagag atttaagtat ctgttttaat aagctctttt
     4561 tgttatttaa aggctgccca tgggtttctg cctagtggta aagctgattg ttaccctcct
     4621 ttgaaatccc ttctagttct gagatgcttt gagggtaact ggattcgatt ttgggatatc
     4681 ttttctcaca ttcagactta cacttaatgg tgttagaaat caacaaaact cctttttaaa
     4741 aagaaaagat attaagcctg cctacttcta caatgcattc tgttacctat ttgaacagta
     4801 tgtttgtaac tatggcaatg aagtcagtag ataggaaacc agttattcct tctaccttta
     4861 aaaattttga gaacttgcca accagggatt aaagctatta tcttgaacag agtccctaaa
     4921 gctagtctag tttttgccac atctgcaatg attattgttt aatttcaaaa gaatcctcag
     4981 gctctacaat ctaggggtgg taaatgtgtt tccactatac ttgggaaaag gtcagtagga
     5041 tgtgcatcct agggaagata aaatcgtata tggtaaaggc atttgagtta attttgcatt
     5101 atatctagga accatattat ttaaaatttg aatcctatta atgctgagag atcctaagag
     5161 ctagtatgtt gtaaaacctg ccacctgaat aaaatgaaaa aaaaagtgtt tttttgagac
     5221 agagtcttgc tctgttgccc aggctggagt gcagtggtgt gatcttgggt cactgcaaac
     5281 tccgcctccc aggttcacgc cattctcctg cctcagcctc ccgagtagct gggaccacag
     5341 gggcccacca ccgcgcccgg ctaatttttt gtatttttag tagagacggg gtttcaccgt
     5401 gttagccagg atgttctcga tctcctgacc tcatgatccg cccgcctcgg cctcccaaag
     5461 tgctgggatt acaggcgtga gccaccgtgc ccggccctaa aatgaaatta ttaatgttgg
     5521 ttctgaaaca gcctcttagc attcagattg tattttaaat acttaaacac accaattgta
     5581 gaaagcaacc ctttattttt agcaagtttg gtttactctt ctcccatgag attttgtctt
     5641 tctacattaa ctagtaagac ataaagattt gaaactggaa ctatattata ctttttaacc
     5701 aatctctgtg gcttcactca tgaaatttac ttcagttaat atgagactgg gggttaagtt
     5761 gtaactaata tgcaaaattg ctttgtatat ttggtgattt tgtaatgtaa gtgaattgat
     5821 tcttatttca ctgatactat taatcatgca gtgtacaatt caaaatcatg caatgtttca
     5881 ctttagaatt ggatttgaag aactcgactt tatgtgatca tggtattggt atacatgtgg
     5941 ggtggagaac ttattttttc attttgtcac aataggtata tactgacaag gcttcaggaa
     6001 aaaagttgtt agaagatttt ttaatgtata ataaagtcca tgatttttgt acagtgtttt
     6061 ttaatgaaa
//
