LOCUS       NM_181861               7201 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens apoptotic peptidase activating factor 1 (APAF1),
            transcript variant 3, mRNA.
ACCESSION   NM_181861
VERSION     NM_181861.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Yuan,Z., Xiong,B., Liu,L., Lu,Y., Liu,Y., Wang,G., Qian,Y., Diao,B.
            and Tu,M.
  TITLE     Exosomal circ_0037104 derived from Hu-MSCs inhibits
            cholangiocarcinoma progression by sponging miR-620 and targeting
            AFAP1
  JOURNAL   J Biochem Mol Toxicol 38 (2), e23656 (2024)
   PUBMED   38348717
  REMARK    GeneRIF: Exosomal circ_0037104 derived from Hu-MSCs inhibits
            cholangiocarcinoma progression by sponging miR-620 and targeting
            AFAP1.
REFERENCE   2  (bases 1 to 7201)
  AUTHORS   Chang,K.H., Liu,C.H., Wang,Y.R., Lo,Y.S., Chang,C.W., Wu,H.C. and
            Chen,C.M.
  TITLE     Upregulation of APAF1 and CSF1R in Peripheral Blood Mononuclear
            Cells of Parkinson's Disease
  JOURNAL   Int J Mol Sci 24 (8), 7095 (2023)
   PUBMED   37108258
  REMARK    GeneRIF: Upregulation of APAF1 and CSF1R in Peripheral Blood
            Mononuclear Cells of Parkinson's Disease.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7201)
  AUTHORS   Wang,Q., Chen,C., Xu,X., Shu,C., Cao,C., Wang,Z., Fu,Y., Xu,L.,
            Xu,K., Xu,J., Xia,A., Wang,B., Xu,G., Zou,X., Su,R., Kang,W.,
            Xue,Y., Mo,R., Sun,B. and Wang,S.
  TITLE     APAF1-Binding Long Noncoding RNA Promotes Tumor Growth and
            Multidrug Resistance in Gastric Cancer by Blocking Apoptosome
            Assembly
  JOURNAL   Adv Sci (Weinh) 9 (28), e2201889 (2022)
   PUBMED   35975461
  REMARK    GeneRIF: APAF1-Binding Long Noncoding RNA Promotes Tumor Growth and
            Multidrug Resistance in Gastric Cancer by Blocking Apoptosome
            Assembly.
REFERENCE   4  (bases 1 to 7201)
  AUTHORS   Mohamed,D.A.W., Selim,H.M., Elmazny,A., Genena,A. and Nabil,M.M.
  TITLE     Apoptotic protease activating factor-1 gene and MicroRNA-484: A
            possible interplay in relapsing remitting multiple sclerosis
  JOURNAL   Mult Scler Relat Disord 58, 103502 (2022)
   PUBMED   35030371
  REMARK    GeneRIF: Apoptotic protease activating factor-1 gene and
            MicroRNA-484: A possible interplay in relapsing remitting multiple
            sclerosis.
REFERENCE   5  (bases 1 to 7201)
  AUTHORS   Shen,C., Tao,C., Zhang,A., Li,X., Guo,Y., Wei,H., Yin,Q., Li,Q. and
            Jin,P.
  TITLE     Exosomal microRNA-93-3p secreted by bone marrow mesenchymal stem
            cells downregulates apoptotic peptidase activating factor 1 to
            promote wound healing
  JOURNAL   Bioengineered 13 (1), 27-37 (2022)
   PUBMED   34898374
  REMARK    GeneRIF: Exosomal microRNA rectangle93 rectangle3p secreted by bone
            marrow mesenchymal stem cells downregulates apoptotic peptidase
            activating factor 1 to promote wound healing.
REFERENCE   6  (bases 1 to 7201)
  AUTHORS   Srinivasula,S.M., Ahmad,M., Fernandes-Alnemri,T. and Alnemri,E.S.
  TITLE     Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization
  JOURNAL   Mol Cell 1 (7), 949-957 (1998)
   PUBMED   9651578
REFERENCE   7  (bases 1 to 7201)
  AUTHORS   Hu,Y., Benedict,M.A., Wu,D., Inohara,N. and Nunez,G.
  TITLE     Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent
            caspase-9 activation
  JOURNAL   Proc Natl Acad Sci U S A 95 (8), 4386-4391 (1998)
   PUBMED   9539746
REFERENCE   8  (bases 1 to 7201)
  AUTHORS   Pan,G., O'Rourke,K. and Dixit,V.M.
  TITLE     Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex
  JOURNAL   J Biol Chem 273 (10), 5841-5845 (1998)
   PUBMED   9488720
REFERENCE   9  (bases 1 to 7201)
  AUTHORS   Li,P., Nijhawan,D., Budihardjo,I., Srinivasula,S.M., Ahmad,M.,
            Alnemri,E.S. and Wang,X.
  TITLE     Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9
            complex initiates an apoptotic protease cascade
  JOURNAL   Cell 91 (4), 479-489 (1997)
   PUBMED   9390557
REFERENCE   10 (bases 1 to 7201)
  AUTHORS   Zou,H., Henzel,W.J., Liu,X., Lutschg,A. and Wang,X.
  TITLE     Apaf-1, a human protein homologous to C. elegans CED-4,
            participates in cytochrome c-dependent activation of caspase-3
  JOURNAL   Cell 90 (3), 405-413 (1997)
   PUBMED   9267021
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC013283.23, AF013263.1,
            AF149794.1 and AC011248.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_181861.1.
            
            Summary: This gene encodes a cytoplasmic protein that initiates
            apoptosis. This protein contains several copies of the WD-40
            domain, a caspase recruitment domain (CARD), and an ATPase domain
            (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an
            oligomeric apoptosome. The apoptosome binds and cleaves caspase 9
            preproprotein, releasing its mature, activated form. Activated
            caspase 9 stimulates the subsequent caspase cascade that commits
            the cell to apoptosis. Alternative splicing results in several
            transcript variants encoding different isoforms. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (3), also known as Apaf-1L,
            encodes the longest isoform (c) of this protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.187595.1,
                                           SRR1803614.51709.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000551964.6/ ENSP00000448165.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                AC013283.23        91900-91909
            11-587              AF013263.1         1-577
            588-4334            AF149794.1         1-3747
            4335-5339           AF013263.1         4163-5167
            5340-5340           AC011248.8         25332-25332         c
            5341-6760           AF013263.1         5169-6588
            6761-6761           AC011248.8         23911-23911         c
            6762-6781           AF013263.1         6590-6609
            6782-6782           AC011248.8         23890-23890         c
            6783-7201           AF013263.1         6611-7029
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q23.1"
     gene            1..7201
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /note="apoptotic peptidase activating factor 1"
                     /db_xref="GeneID:317"
                     /db_xref="HGNC:HGNC:576"
                     /db_xref="MIM:602233"
     exon            1..546
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            547..725
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     CDS             588..4334
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /note="isoform c is encoded by transcript variant 3;
                     apoptotic protease-activating factor 1"
                     /codon_start=1
                     /product="apoptotic protease-activating factor 1 isoform
                     c"
                     /protein_id="NP_863651.1"
                     /db_xref="CCDS:CCDS9069.1"
                     /db_xref="GeneID:317"
                     /db_xref="HGNC:HGNC:576"
                     /db_xref="MIM:602233"
                     /translation="MDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLTISEEEKVR
                     NEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAALLHDGIPVVSSSSGKDS
                     VSGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGK
                     SVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPL
                     NIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVTDSVMGP
                     KYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDF
                     PNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKD
                     VKVPTKVLCILWDMETEEVEDILQEFVNKSLLFCDRNGKSFRYYLHDLQVDFLTEKNC
                     SQLQDLHKKIITQFQRYHQPHTLSPDQEDCMYWYNFLAYHMASAKMHKELCALMFSLD
                     WIKAKTELVGPAHLIHEFVEYRHILDEKDCAVSENFQEFLSLNGHLLGRQPFPNIVQL
                     GLCEPETSEVYQQAKLQAKQEVDNGMLYLEWINKKNITNLSRLVVRPHTDAVYHACFS
                     EDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSTDDRFIATCSVDKKVK
                     IWNSMTGELVHTYDEHSEQVNCCHFTNSSHHLLLATGSSDCFLKLWDLNQKECRNTMF
                     GHTNSVNHCRFSPDDKLLASCSADGTLKLWDATSANERKSINVKQFFLNLEDPQEDME
                     VIVKCCSWSADGARIMVAAKNKIFLFDIHTSGLLGEIHTGHHSTIQYCDFSPQNHLAV
                     VALSQYCVELWNTDSRSKVADCRGHLSWVHGVMFSPDGSSFLTSSDDQTIRLWETKKV
                     CKNSAVMLKQEVDVVFQENEVMVLAVDHIRRLQLINGRTGQIDYLTEAQVSCCCLSPH
                     LQYIAFGDENGAIEILELVNNRIFQSRFQHKKTVWHIQFTADEKTLISSSDDAEIQVW
                     NWQLDKCIFLRGHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITGNKEKDFVCHQGTV
                     LSCDISHDATKFSSTSADKTAKIWSFDLLLPLHELRGHNGCVRCSAFSVDSTLLATGD
                     DNGEIRIWNVSNGELLHLCAPLSEEGAATHGGWVTDLCFSPDGKMLISAGGYIKWWNV
                     VTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILQTLE"
     exon            726..915
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            916..1113
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1297
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1410
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1542
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1781
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            1782..1949
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..2081
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            2082..2195
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            2196..2380
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            2381..2507
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            2508..2633
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            2634..2765
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            2766..2891
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            2892..3053
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            3054..3182
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            3183..3308
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            3309..3428
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            3429..3545
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            3546..3671
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            3672..3791
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            3792..3917
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            3918..4043
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            4044..4187
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     exon            4188..7201
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7171..7176
                     /regulatory_class="polyA_signal_sequence"
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /note="hexamer: AATAAA"
     polyA_site      7201
                     /gene="APAF1"
                     /gene_synonym="APAF-1; CED4"
                     /note="major polyA site"
ORIGIN      
        1 agaggcggag aagaagaggt agcgagtgga cgtgactgct ctatcccggg caaaagggat
       61 agaaccagag gtggggagtc tgggcagtcg gcgacccgcg aagacttgag gtgccgcagc
      121 ggcatccgga gtagcgccgg gctccctccg gggtgcagcc gccgtcgggg gaagggcgcc
      181 acaggccggg aagacctcct ccctttgtgt ccagtagtgg ggtccaccgg agggcggccc
      241 gtgggccggg cctcaccgcg gcgctccggg actgtggggt caggctgcgt tgggtggacg
      301 cccacctcgc caaccttcgg aggtccctgg gggtcttcgt gcgccccggg gctgcagaga
      361 tccaggggag gcgcctgtga ggcccggacc tgccccgggg cgaagggtat gtggcgagac
      421 agagccctgc acccctaatt cccggtggaa aactcctgtt gccgtttccc tccaccggcc
      481 tggagtctcc cagtcttgtc ccggcagtgc cgccctcccc actaagacct aggcgcaaag
      541 gcttggctca tggttgacag ctcagagaga gaaagatctg agggaagatg gatgcaaaag
      601 ctcgaaattg tttgcttcaa catagagaag ctctggaaaa ggacatcaag acatcctaca
      661 tcatggatca catgattagt gatggatttt taacaatatc agaagaggaa aaagtaagaa
      721 atgagcccac tcaacagcaa agagcagcta tgctgattaa aatgatactt aaaaaagata
      781 atgattccta cgtatcattc tacaatgctc tactacatga aggatataaa gatcttgctg
      841 cccttctcca tgatggcatt cctgttgtct cttcttccag tggtaaagat tcagttagtg
      901 gaataacttc gtatgtaagg acagtcctgt gtgaaggtgg agtaccacag aggccagttg
      961 tttttgtcac aaggaagaag ctggtgaatg caattcagca gaagctctcc aaattgaaag
     1021 gtgaaccagg atgggtcacc atacatggaa tggcaggctg tgggaagtct gtattagctg
     1081 cagaagctgt tagagatcat tcccttttag aaggttgttt cccaggggga gtgcattggg
     1141 tttcagttgg gaaacaagac aaatctgggc ttctgatgaa actgcagaat ctttgcacac
     1201 ggttggatca ggatgagagt ttttcccaga ggcttccact taatattgaa gaggctaaag
     1261 accgtctccg cattctgatg cttcgcaaac acccaaggtc tctcttgatc ttggatgatg
     1321 tttgggactc ttgggtgttg aaagcttttg acagtcagtg tcagattctt cttacaacca
     1381 gagacaagag tgttacagat tcagtaatgg gtcctaaata tgtagtccct gtggagagtt
     1441 ccttaggaaa ggaaaaagga cttgaaattt tatccctttt tgttaatatg aagaaggcag
     1501 atttgccaga acaagctcat agtattataa aagaatgtaa aggctctccc cttgtagtat
     1561 ctttaattgg tgcactttta cgtgattttc ccaatcgctg ggagtactac ctcaaacagc
     1621 ttcagaataa gcagtttaag agaataagga aatcttcgtc ttatgattat gaggctctag
     1681 atgaagccat gtctataagt gttgaaatgc tcagagaaga catcaaagat tattacacag
     1741 atctttccat ccttcagaag gacgttaagg tgcctacaaa ggtgttatgt attctctggg
     1801 acatggaaac tgaagaagtt gaagacatac tgcaggagtt tgtaaataag tctcttttat
     1861 tctgtgatcg gaatggaaag tcgtttcgtt attatttaca tgatcttcaa gtagattttc
     1921 ttacagagaa gaattgcagc cagcttcagg atctacataa gaagataatc actcagtttc
     1981 agagatatca ccagccgcat actctttcac cagatcagga agactgtatg tattggtaca
     2041 actttctggc ctatcacatg gccagtgcca agatgcacaa ggaactttgt gctttaatgt
     2101 tttccctgga ttggattaaa gcaaaaacag aacttgtagg ccctgctcat ctgattcatg
     2161 aatttgtgga atacagacat atactagatg aaaaggattg tgcagtcagt gagaattttc
     2221 aggagttttt atctttaaat ggacaccttc ttggacgaca gccatttcct aatattgtac
     2281 aactgggtct ctgtgagccg gaaacttcag aagtttatca gcaagctaag ctgcaggcca
     2341 agcaggaggt cgataatgga atgctttacc tggaatggat aaacaaaaaa aacatcacga
     2401 atctttcccg cttagttgtc cgcccccaca cagatgctgt ttaccatgcc tgcttttctg
     2461 aggatggtca gagaatagct tcttgtggag ctgataaaac cttacaggtg ttcaaagctg
     2521 aaacaggaga gaaacttcta gaaatcaagg ctcatgagga tgaagtgctt tgttgtgcat
     2581 tctctacaga tgacagattt atagcaacct gctcagtgga taaaaaagtg aagatttgga
     2641 attctatgac tggggaacta gtacacacct atgatgagca ctcagagcaa gtcaattgct
     2701 gccatttcac caacagtagt catcatcttc tcttagccac tgggtcaagt gactgcttcc
     2761 tcaaactttg ggatttgaat caaaaagaat gtcgaaatac catgtttggt catacaaatt
     2821 cagtcaatca ctgcagattt tcaccagatg ataagctttt ggctagttgt tcagctgatg
     2881 gaaccttaaa gctttgggat gcgacatcag caaatgagag gaaaagcatt aatgtgaaac
     2941 agttcttcct aaatttggag gaccctcaag aggatatgga agtgatagtg aagtgttgtt
     3001 cgtggtctgc tgatggtgca aggataatgg tggcagcaaa aaataaaatc tttctttttg
     3061 acattcatac tagtggccta ttgggagaaa tccacacggg ccatcacagc accatccagt
     3121 actgtgactt ctccccacaa aaccatttgg cagtggttgc tttgtcccag tactgtgtag
     3181 agttgtggaa tacagactca cgttcaaagg tggctgattg cagaggacat ttaagttggg
     3241 ttcatggtgt gatgttttct cctgatggat catcattttt gacatcttct gatgaccaga
     3301 caatcaggct ctgggagaca aagaaagtat gtaagaactc tgctgtaatg ttaaagcaag
     3361 aagtagatgt tgtgtttcaa gaaaatgaag tgatggtcct tgcagttgac catataagac
     3421 gtctgcaact cattaatgga agaacaggtc agattgatta tctgactgaa gctcaagtta
     3481 gctgctgttg cttaagtcca catcttcagt acattgcatt tggagatgaa aatggagcca
     3541 ttgagatttt agaacttgta aacaatagaa tcttccagtc caggtttcag cacaagaaaa
     3601 ctgtatggca catccagttc acagccgatg agaagactct tatttcaagt tctgatgatg
     3661 ctgaaattca ggtatggaat tggcaattgg acaaatgtat ctttctacga ggccatcagg
     3721 aaacagtgaa agactttaga ctcttgaaaa attcaagact gctttcttgg tcatttgatg
     3781 gaacagtgaa ggtatggaat attattactg gaaataaaga aaaagacttt gtctgtcacc
     3841 agggtacagt actttcttgt gacatttctc acgatgctac caagttttca tctacctctg
     3901 ctgacaagac tgcaaagatc tggagttttg atctcctttt gccacttcat gaattgaggg
     3961 gccacaacgg ctgtgtgcgc tgctctgcct tctctgtgga cagtaccctg ctggcaacgg
     4021 gagatgacaa tggagaaatc aggatatgga atgtctcaaa cggtgagctt cttcatttgt
     4081 gtgctccgct ttcagaagaa ggagctgcta cccatggagg ctgggtgact gacctttgct
     4141 tttctccaga tggcaaaatg cttatctctg ctggaggata tattaagtgg tggaacgttg
     4201 tcactgggga atcctcacag accttctaca caaatggaac caatcttaag aaaatacacg
     4261 tgtcccctga cttcaaaaca tatgtgactg tggataatct tggtatttta tatattttac
     4321 agactttaga ataaaatagt taagcattaa tgtagttgaa ctttttaaat ttttgaattg
     4381 gaaaaaaatt ctaatgaaac cctgatatca actttttata aagctcttaa ttgttgtgca
     4441 gtattgcatt cattacaaaa gtgtttgtgg ttggatgaat aatattaatg tagctttttc
     4501 ccaaatgaac atacctttaa tcttgttttt catgatcatc attaacagtt tgtccttagg
     4561 atgcaaatga aaatgtgaat acataccttg ttgtactgtt ggtaaaattc tgtcttgatg
     4621 cattcaaaat ggttgacata attaatgaga agaatttgga agaaattggt attttaatac
     4681 tgtctgtatt tattactgtt atgcaggctg tgcctcaggg tagcagtggc ctgctttttg
     4741 aaccacactt accccaaggg ggttttgttc tcctaaatac aatcttagag gttttttgca
     4801 ctctttaaat ttgctttaaa aatattgtgt ctgtgtgcat agtctgcagc atttccttta
     4861 attgactcaa taagtgagtc ttggatttag caggcccccc cacctttttt ttttgttttt
     4921 ggagacagag tcttgctttg ttgccaggct ggagtgcagt ggcgcgatct cggctcacca
     4981 caatcgctgc ctcctgggtt caagcaattc tcctgcctca gcctcccgag tagctgggac
     5041 tacaggtgtg cgcacatgcc aggctaattt ttgtattttt agtagagacg gggtttcacc
     5101 atgttggccg ggatggtctc gatctcttga cctcatgatc tacccgcctt ggcctcccaa
     5161 agtgctgaga ttacaggcgt gagccaccgt gcctggccag gccccttctc ttttaatgga
     5221 gacagggtct tgcactatca cccaggctgg agtgcagtgg cataatcata cctcattgca
     5281 gcctcagact cctgggttca agcaatcctc ctgcctcagc ctcccaagta gctgagacta
     5341 caggcacgag ccaccacacc cagctaattt ttaagttttc ttgtagagac agggtctcac
     5401 tatgttgtct aggctggtct tgaactcttg gcctcaagta atcctcctgc ctcagcctcc
     5461 caaagtgttg ggattgcaga tatgagccac tggcctggcc ttcagcagtt ctttttgtga
     5521 agtaaaactt gtatgttgga aagagtagat tttattggtc tacccttttc tcactgtagc
     5581 tgctggcagc cctgtgccat atctggactc tagttgtcag tatctgagtt ggacactatt
     5641 cctgctccct cttgtttctt acatatcaga cttcttactt gaatgaaacc tgatctttcc
     5701 taatcctcac ttttttcttt tttaaaaagc agtttctcca ctgctaaatg ttagtcattg
     5761 aggtggggcc aattttaatc ataagcctta ataagatttt tctaagaaat gtgaaataga
     5821 acaattttca tctaattcca tttactttta gatgaatggc attgtgaatg ccattctttt
     5881 aatgaatttc aagagaattc tctggttttc tgtgtaattc cagatgagtc actgtaactc
     5941 tagaagatta accttccagc caacctattt tcctttccct tgtctctctc atcctctttt
     6001 ccttccttct ttcctttctc ttcttttatc tccaaggtta atcaggaaaa atagcttttg
     6061 acaggggaaa aaactcaata actagctatt tttgacctcc tgatcaggaa ctttagttga
     6121 agcgtaaatc taaagaaaca ttttctctga aatatattat taagggcaat ggagataaat
     6181 taatagtaga tgtggttccc agaaaatata atcaaaattc aaagattttt tttgtttctg
     6241 taactggaac taaatcaaat gattactagt gttaatagta gataacttgt ttttattgtt
     6301 ggtgcatatt agtataactg tggggtaggt cggggagagg gtaagggaat agatcactca
     6361 gatgtatttt agataagcta tttagccttt gatggaatca taaatacagt gaatacaatc
     6421 ctttgcattg ttaaggaggt tttttgtttt taaatggtgg gtcaaggagc tagtttacag
     6481 gcttactgtg atttaagcaa atgtgaaaag tgaaacctta attttatcaa aagaaatttc
     6541 tgtaaatggt atgtctcctt agaataccca aatcataatt ttatttgtac acactgttag
     6601 gggctcatct catgtaggca gagtataaag tattaccttt tggaattaaa agccactgac
     6661 tgttataaag tataacaaca cacatcaggt tttaaaaagc cttgaatggc ccttgtctta
     6721 aaaagaaatt aggagccagg tgcggtggca cgtgcctgta atcccagctc cttgggaggc
     6781 taagacagga ggattccttg agccctggag tttgagtcca gcctgggtga catagcaaga
     6841 ccctgtctta aaagaaaaat gggaagaaag acaaggtaac atgaagaaag aagagatacc
     6901 tagtatgatg gagctgcaaa tttcatggca gttcatgcag tcggtcaaga ggaggatttt
     6961 gttttgtagt ttgcagatga gcatttctaa agcattttcc cttgctgtat ttttttgtat
     7021 tataaattac attggacttc atatatataa ttttttttta cattatatgt ctcttgtatg
     7081 ttttgaaact cttgtattta tgatatagct tatatgattt ttttgccttg gtatacattt
     7141 taaaatatga atttaaaaaa tttttgtaaa aataaaattc acaaaattgt tttgaaaaac
     7201 a
//
