LOCUS       NM_014855               5529 bp    mRNA    linear   PRI 06-OCT-2024
DEFINITION  Homo sapiens adaptor related protein complex 5 subunit zeta 1
            (AP5Z1), transcript variant 1, mRNA.
ACCESSION   NM_014855 XM_166527 XM_379771 XM_499251 XM_499396
VERSION     NM_014855.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Denton,K., Mou,Y., Xu,C.C., Shah,D., Chang,J., Blackstone,C. and
            Li,X.J.
  TITLE     Impaired mitochondrial dynamics underlie axonal defects in
            hereditary spastic paraplegias
  JOURNAL   Hum Mol Genet 27 (14), 2517-2530 (2018)
   PUBMED   29726929
  REMARK    GeneRIF: Here, we have generated induced pluripotent stem cells
            (iPSCs) from patients with two autosomal recessive forms of
            hereditary spastic paraplegia (HSP) , SPG15 and SPG48, which are
            caused by mutations in the ZFYVE26 and AP5Z1 genes encoding
            proteins in the same complex, the spastizin and AP5Z1 proteins,
            respectively.
REFERENCE   3  (bases 1 to 5529)
  AUTHORS   Hirst,J., Barlow,L.D., Francisco,G.C., Sahlender,D.A., Seaman,M.N.,
            Dacks,J.B. and Robinson,M.S.
  TITLE     The fifth adaptor protein complex
  JOURNAL   PLoS Biol 9 (10), e1001170 (2011)
   PUBMED   22022230
  REMARK    Erratum:[PLoS Biol. 2012 Mar;10(3). doi:
            10.1371/annotation/89dff893-c156-44bb-a731-bfcc91843583]
REFERENCE   4  (bases 1 to 5529)
  AUTHORS   Slabicki,M., Theis,M., Krastev,D.B., Samsonov,S., Mundwiller,E.,
            Junqueira,M., Paszkowski-Rogacz,M., Teyra,J., Heninger,A.K.,
            Poser,I., Prieur,F., Truchetto,J., Confavreux,C., Marelli,C.,
            Durr,A., Camdessanche,J.P., Brice,A., Shevchenko,A.,
            Pisabarro,M.T., Stevanin,G. and Buchholz,F.
  TITLE     A genome-scale DNA repair RNAi screen identifies SPG48 as a novel
            gene associated with hereditary spastic paraplegia
  JOURNAL   PLoS Biol 8 (6), e1000408 (2010)
   PUBMED   20613862
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5529)
  AUTHORS   Wistow,G., Bernstein,S.L., Ray,S., Wyatt,M.K., Behal,A.,
            Touchman,J.W., Bouffard,G., Smith,D. and Peterson,K.
  TITLE     Expressed sequence tag analysis of adult human iris for the NEIBank
            Project: steroid-response factors and similarities with retinal
            pigment epithelium
  JOURNAL   Mol Vis 8, 185-195 (2002)
   PUBMED   12107412
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5529)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY145300.1, BI766915.1,
            CD671081.1, BQ228738.1, BC008841.2, AB007875.1, AC092610.1 and
            AI927641.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jul 11, 2018 this sequence version replaced NM_014855.2.
            
            Summary: This gene was identified by genome-wide screen for genes
            involved in homologous recombination DNA double-strand break repair
            (HR-DSBR). The encoded protein was found in a complex with other
            proteins that have a role in HR-DSBR. Knockdown of this gene
            reduced homologous recombination, and mutations in this gene were
            found in patients with spastic paraplegia. It was concluded that
            this gene likely encodes a helicase (PMID:20613862). [provided by
            RefSeq, Jan 2011].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.81952.1,
                                           SRR1660805.176643.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000649063.2/ ENSP00000497815.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 HY145300.1         3-10
            9-638               BI766915.1         1-630
            639-1061            CD671081.1         55-477
            1062-1665           BQ228738.1         80-683
            1666-2888           BC008841.2         326-1548
            2889-5357           AB007875.1         3105-5573
            5358-5358           AC092610.1         49383-49383
            5359-5527           AB007875.1         5575-5743
            5528-5529           AI927641.1         3-4                 c
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p22.1"
     gene            1..5529
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /note="adaptor related protein complex 5 subunit zeta 1"
                     /db_xref="GeneID:9907"
                     /db_xref="HGNC:HGNC:22197"
                     /db_xref="MIM:613653"
     exon            1..134
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     CDS             94..2517
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /note="isoform 1 is encoded by transcript variant 1;
                     zeta5; adapter-related protein complex 5 zeta subunit;
                     AP-5 complex subunit zeta-1; adaptor related protein
                     complex 5 zeta 1 subunit; adaptor-related protein complex
                     5 zeta subunit"
                     /codon_start=1
                     /product="AP-5 complex subunit zeta-1 isoform 1"
                     /protein_id="NP_055670.1"
                     /db_xref="CCDS:CCDS47528.1"
                     /db_xref="GeneID:9907"
                     /db_xref="HGNC:HGNC:22197"
                     /db_xref="MIM:613653"
                     /translation="MFSAGAESLLHQAREIQDEELKKFCSRICKLLQAEDLGPDTLDS
                     LQRLFLIISATKYSRRLEKTCVDLLQATLGLPACPEQLQVLCAAILREMSPSDSLSLA
                     WDHTQNSRQLSLVASVLLAQGDRNEEVRAVGQGVLRALESRQPEGPSLRHLLPVMAKV
                     VVLSPGTLQEDQATLLSKRLVDWLRYASLQQGLPHSGGFFSTPRARQPGPVTEVDGAV
                     ATDFFTVLSSGHRFTDDQWLNVQAFSMLRAWLLHSGPEGPGTLDTDDRSEQEGSTLSV
                     ISATSSAGRLLPPRERLREVAFEYCQRLIEQSNRRALRKGDSDLQKACLVEAVLVLDV
                     LCRQDPSFLYRSLSCLKALHGRVRGDPASVRVLLPLAHFFLSHGEAAAVDSEAVYQHL
                     FTRIPVEQFHSPMLAFEFIQFCRDNLHLFSGHLSTLRLSFPNLFKFLAWNSPPLTSEF
                     VALLPALVDAGTALEMLHALLDLPCLTAVLDLQLRSAPAASERPLWDTSLRAPSCLEA
                     FRDPQFQGLFQYLLRPKASGATERLAPLHQLLQPMAGCARVAQCAQAVPTLLQAFFSA
                     VTQVADGSLINQLALLLLGRSDSLYPAPGYAAGVHSVLSSQFLALCTLKPSLVVELAR
                     DLLEFLGSVNGLCSRASLVTSVVWAIGEYLSVTYDRRCTVEQINKFFEALEALLFEVT
                     QCRPSAALPRCPPQVVTVLMTTLTKLASRSQDLIPRASLLLSKMRTLAHSPATSSTHS
                     EEGAEAIRTRATELLTLLKMPSVAQFVLTPSTEVCSPRYHRDANTALPLALRTVSRLV
                     EREAGLMPG"
     exon            135..272
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            273..459
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            460..604
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            605..714
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            715..883
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            884..1024
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1025..1062
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1225
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1404
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1405..1547
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1688
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1689..1800
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..1898
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..2031
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2032..2246
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2247..5529
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2863..2868
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /note="hexamer: AGTAAA"
     regulatory      2869..2874
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /note="hexamer: AGTAAA"
     polyA_site      2884
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /note="major polyA site"
     polyA_site      2888
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
     regulatory      5508..5513
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
                     /note="hexamer: AATAAA"
     polyA_site      5528
                     /gene="AP5Z1"
                     /gene_synonym="KIAA0415; SPG48; zeta"
ORIGIN      
        1 gacgcggtcc cggaagttga ccggggtgcg gagctcctgg gctgcagctc ctggagtttc
       61 cgaggttcgt gcgcgtctgg tggcggcggc gtgatgttct cggcaggagc ggagagtttg
      121 ctccaccagg ccagggagat ccaggacgag gagctgaaga agttctgttc ccggatctgt
      181 aaactgctgc aggcggagga cttggggccg gacaccctcg actccctgca gaggctcttc
      241 ctcatcatct cagccacgaa gtacagccgg aggctggaga agacatgcgt agacctgctg
      301 caggccaccc tcggcctgcc tgcatgcccc gagcagctcc aggtgctttg cgccgccatc
      361 ctgcgagaga tgtccccctc tgacagcctc agcctggcct gggaccacac gcagaacagc
      421 cggcagctga gcctggtggc ctccgttctc ttggcccagg gtgacagaaa cgaggaggtc
      481 agagccgtgg gccagggcgt gctacgagcg ctggagagcc ggcagcctga gggacccagc
      541 ctcagacacc tcctccccgt catggccaag gtcgtggtcc tcagcccggg caccctccag
      601 gaggaccagg ccaccctgct cagcaagcgg ctggtcgact ggctgcgcta cgccagcctc
      661 cagcaagggc tcccacactc cggcggcttc ttctccacgc ccagggcccg gcagccgggc
      721 cccgtcaccg aggtggacgg ggcggtagcc acagacttct tcacggtgct ctccagcggc
      781 caccgcttca cagacgacca gtggctgaac gtgcaggcct tctctatgct gcgggcgtgg
      841 ctgctgcaca gcggccccga gggcccgggc accctggaca cagatgacag gtcagagcag
      901 gagggctcca ctctgtcggt gatctccgcc acctcctctg ccggccgcct gctgccgccc
      961 cgggagcggc ttcgggaggt ggccttcgag tactgccagc gcctcattga gcaaagtaac
     1021 cgacgagccc tgaggaaggg ggactccgac ctgcagaaag cttgcctggt ggaggccgtg
     1081 ctggtgctgg acgtgctgtg ccggcaggac ccgtccttcc tgtaccgaag tctctcctgc
     1141 ctgaaggccc tgcacgggcg ggtgcgcggg gacccggcct ctgtgcgggt gctgctgccc
     1201 ctcgcccact tcttcctgag ccacggggaa gcggctgcag tggactcgga agccgtctac
     1261 cagcacctgt tcaccaggat cccggtggag cagttccaca gccccatgct ggcctttgaa
     1321 ttcatccagt tctgcaggga caacctccac ctgttcagcg ggcacctcag caccctcaga
     1381 ttgagcttcc ccaacctctt taagttcctg gcctggaaca gcccacccct cacctccgag
     1441 tttgtggcgc tcctcccggc cctggtggac gctggcacag ccctggagat gctgcacgcg
     1501 ctgctggacc tgccctgctt gacggcggtg ctggacctgc agctcaggtc agcaccggct
     1561 gcatccgaga ggccactctg ggacacctct ctcagggccc ccagctgcct ggaggccttc
     1621 cgggacccgc agttccaggg tcttttccaa tacctgctgc gccccaaggc cagtggcgcc
     1681 actgagaggt tggcgccact ccaccagctg ctgcagccca tggccggctg tgcccgcgtg
     1741 gcccagtgtg cccaggccgt gcccacgctg ctgcaggcat tcttctcagc agtgacccag
     1801 gtggctgacg ggtccctgat caaccagctg gcgctgctgc tcctgggcag gagcgactcg
     1861 ctctacccgg ccccagggta cgctgccggt gtgcacagtg tgctgagttc tcagttcctg
     1921 gccctgtgta cgctgaaacc ctccctggtg gtggagctgg caagagacct gctggagttc
     1981 ctgggcagcg tgaatggtct ctgcagcagg gcgagcctcg tcaccagcgt ggtgtgggcc
     2041 atcggcgagt acctgtcggt gacctacgat cggaggtgca ccgtggagca gatcaacaag
     2101 ttcttcgaag ccctggaggc tctgctattc gaggtcaccc agtgccgccc ctctgctgcc
     2161 ctgcccaggt gtccccccca ggtggtcacc gtgctgatga ccacgctgac gaagctggcc
     2221 tcccggagcc aagatctgat ccccagggcc tctttattgc tgtcaaagat gaggaccctg
     2281 gctcacagtc cagccaccag ctccacgcac agcgaggagg gcgcggaagc catccgtacc
     2341 cgggccacag agctgctgac cctgctgaag atgcctagcg tggcccagtt tgtgctcaca
     2401 cccagcacgg aggtgtgcag cccccgctat caccgcgatg ccaacacggc cctgcccctg
     2461 gccctgcgca cggtcagccg gctggtggag agggaggccg gcctcatgcc agggtgaagg
     2521 gacagtggcc agggacttcg gtgcagatta agagcctggg cagccagctt gctactgagg
     2581 ccaggctgat aggagctcag gagggcgcgg gagtcctggg agaggaggca aggcccacgg
     2641 tgggcttggc accctcacag acacgcgggg ctggcccccc tgctcaccct ctgggctttg
     2701 tctccgagcc ttttgctccc aggcaacact gagctgagct gaggggtgcc atggagcggc
     2761 tctgattgga ggcttgaggc cctgtggctg ggtcgggtgg aggctgctgg gtctgtttcc
     2821 tagtcttttg tttttggaca gttgatgggc aagaagagct gcagtaaaag taaatctcct
     2881 ttaataagcg tctgtatgaa gagtgcgcga tcagttccgt tacctgccct gcaccctggg
     2941 gagaggacca gggatgggaa cccctggcag ctgctgccag tcctggaggc tcagcccggc
     3001 gtgccactgc tgctacagaa accaggtcat ttgtgcacag cccggaaggc agcggcgcga
     3061 gttcccgggt gtggtcaccg ctctgggtgg tgcgcttgtc aatgcccctt ttgatgccct
     3121 tgaatagcct gaagatgaag gggaccgggg gagggttgca agcgccatgg cctatgcagg
     3181 ctgcggcctc tggccattcg gagggaaggc cccagggagc cacctgagtg gagctcagaa
     3241 cactggattc gcgcgaaggg gaggtgtctg ggcgtgcggc cccagccccg ccaccttcct
     3301 gggccctgtg gtcccaccct ccggactacc aaggcacagc tgtgtcgcac gttccgcccg
     3361 gtgcctggga cgggctcagc cgccaggggg cgccgccgcc ccgagagcct cacgccccgc
     3421 ccccaggctc aaactcttcc ccctccccac tctggctccg cccccggtct cctcttttgc
     3481 tctggccccg cccacctccc ctccggcctc ggccccgccc ccaatccccc atagctctgc
     3541 gactaagaaa ccacaggctg aaggcgactg cagggtcctg ccccttgccc agcctcagga
     3601 ctatggaggg ggcgagtgac gcgcaaggaa cggagcaggg gacaccgtgc gggctcagga
     3661 gctccccaga tgcccgagcc gaaccgcggg gtcctcaccg ccaggttcca gcccaggaag
     3721 cgctcgcctg ccccacagcc tgggccttgg gtcgcgttcc gagctccagg acggctggtg
     3781 tcccccgcgg cctgcgatga cagcccaggg ccgctgagcc ggggccgcag gaaaggctgg
     3841 cgctggcagg cgcttccgtc ggcagcactg tgtttgtgtt tccacgtgga aacagcagcg
     3901 cacgtgtaca actgtgcata ccaaggcgtg cgtgccagtg cgcgggtctc gggatcactt
     3961 cccagtgcgg tgcacgaccc cgcctgcagc agtgggtgtg ggggcgctgc tgggctcatc
     4021 ccgaagctca gagagcgtgg ggctgcccct aggctcggaa tgagtctccc actgagtccg
     4081 cagctcccct cctgccctgg ggcggggctt ccctgacctg aaggcgtcgg gggtgtcctg
     4141 gctcccagct cccaggcact cgtggggcca ccgcccaggc cagctggtgc taagtccgca
     4201 gcctgtgcag cagcgcccgg cttaggactg ggagtgtgac ttgaagggcc gtcccgctca
     4261 tccaagggac aaggaggagc tcatggccac agggcctcgg agggcatgac cccagcccag
     4321 ggaggagcaa ccttcaggtg gcctccagga ggcagctggg aaaagggcaa agctcaccag
     4381 ctctcaactt tttttttaaa taaactgaaa ttttagaata gtttttcaga tttacagaaa
     4441 agatgataaa ccttagaaac attatatgct aagtgaaagt gagaagtgac agcctgctgg
     4501 cagtcctcac agccctcgct cgctcttggc gcctcctctg cctgggctcc cactttggca
     4561 gcacttgagg agcccttcag cccgccactg cactgtggga gcccctttca gggctggcca
     4621 aggccggagc cggctccctc agcttgcggg gaggtgtgga gggagaggcg cggtgggaac
     4681 cagggctgca cgcggtgctt gcgggccagc gcgagttccg ggtgggcgtg ggctctgtgg
     4741 gccccgcact cggagccgcc ggctagcccc gccagcctgg gcagtgaggg gcttagcacc
     4801 tggaccagca gctgctgtgc tcgatttctc actgggcctt agctgccttc ctgcggggca
     4861 gggctgggga cctgcagccc gccatgcctg agcctccccc ctcccctccg tgggttcctg
     4921 cgcagtccga gcctccccaa cgagtgttgc cacctgctca gggcacccag tcccatcgac
     4981 cacccaaggg ctgaggagtg cctttggagc agggcacggg actggcaggc agctctacct
     5041 gcagcccctg tgcaggatcc actgggtgaa agccagctgg gctcctgagt ctggtgggga
     5101 cttggagaac ctttatgtct agctaaggga ttgtaaataa accaattggc actctgtatc
     5161 tagctcaagg tttgtaaaca caccaatcag caccttgtgt ctagctcagg gtttgtgaat
     5221 gcaccaatag acactctgta tctagctact ctggtgggga cttggaaaac ctttttgtca
     5281 acactctgta tctagttaat ctggtgggga catggagaac ctttgtgtct agctcaggga
     5341 ttgtaaacgc accaatcagc accctgtcaa aacagaccac tcggctctac cagtcagcag
     5401 gatgtgggtg gggccagatg agaataaaag caggctgccc gagccagcag tgacaaccca
     5461 ctcgggtccc cttccacacc gggatgcttt gtttgttcgc tctttgcaat aaatcttgct
     5521 gctgctcac
//
