LOCUS       NM_007347               6743 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens adaptor related protein complex 4 subunit epsilon 1
            (AP4E1), transcript variant 1, mRNA.
ACCESSION   NM_007347
VERSION     NM_007347.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Caracci,M.O., Pizarro,H., Alarcon-Godoy,C., Fuentealba,L.M.,
            Farfan,P., De Pace,R., Santibanez,N., Cavieres,V.A., Pastor,T.P.,
            Bonifacino,J.S., Mardones,G.A. and Marzolo,M.P.
  TITLE     The Reelin receptor ApoER2 is a cargo for the adaptor protein
            complex AP-4: Implications for Hereditary Spastic Paraplegia
  JOURNAL   Prog Neurobiol 234, 102575 (2024)
   PUBMED   38281682
  REMARK    GeneRIF: The Reelin receptor ApoER2 is a cargo for the adaptor
            protein complex AP-4: Implications for Hereditary Spastic
            Paraplegia.
            Erratum:[Prog Neurobiol. 2024 Oct;241:102662. doi:
            10.1016/j.pneurobio.2024.102662.. PMID: 39294072]
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 6743)
  AUTHORS   Winkler,I., Miotla,P., Lejman,M., Pietrzyk,A., Kacprzak,M.,
            Kubiak,M., Sobczynska-Tomaszewska,A., Skrzypczak,M. and Jaszczuk,I.
  TITLE     A new family with spastic paraplegia type 51 and novel mutations in
            AP4E1
  JOURNAL   BMC Med Genomics 14 (1), 131 (2021)
   PUBMED   34006278
  REMARK    GeneRIF: A new family with spastic paraplegia type 51 and novel
            mutations in AP4E1.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6743)
  AUTHORS   Moreno-De-Luca,A., Helmers,S.L., Mao,H., Burns,T.G., Melton,A.M.,
            Schmidt,K.R., Fernhoff,P.M., Ledbetter,D.H. and Martin,C.L.
  TITLE     Adaptor protein complex-4 (AP-4) deficiency causes a novel
            autosomal recessive cerebral palsy syndrome with microcephaly and
            intellectual disability
  JOURNAL   J Med Genet 48 (2), 141-144 (2011)
   PUBMED   20972249
  REMARK    GeneRIF: An autosomal recessive form of spastic tetraplegic
            cerebral palsy with profound intellectual disability, microcephaly,
            epilepsy and white matter loss in a consanguineous family resulting
            from a homozygous deletion involving AP4E1.
REFERENCE   5  (bases 1 to 6743)
  AUTHORS   Boehm,M., Aguilar,R.C. and Bonifacino,J.S.
  TITLE     Functional and physical interactions of the adaptor protein complex
            AP-4 with ADP-ribosylation factors (ARFs)
  JOURNAL   EMBO J 20 (22), 6265-6276 (2001)
   PUBMED   11707398
REFERENCE   6  (bases 1 to 6743)
  AUTHORS   Takatsu,H., Futatsumori,M., Yoshino,K., Yoshida,Y., Shin,H.W. and
            Nakayama,K.
  TITLE     Similar subunit interactions contribute to assembly of clathrin
            adaptor complexes and COPI complex: analysis using yeast
            three-hybrid system
  JOURNAL   Biochem Biophys Res Commun 284 (4), 1083-1089 (2001)
   PUBMED   11409905
REFERENCE   7  (bases 1 to 6743)
  AUTHORS   Hirst,J., Bright,N.A., Rous,B. and Robinson,M.S.
  TITLE     Characterization of a fourth adaptor-related protein complex
  JOURNAL   Mol Biol Cell 10 (8), 2787-2802 (1999)
   PUBMED   10436028
REFERENCE   8  (bases 1 to 6743)
  AUTHORS   Dell'Angelica,E.C., Mullins,C. and Bonifacino,J.S.
  TITLE     AP-4, a novel protein complex related to clathrin adaptors
  JOURNAL   J Biol Chem 274 (11), 7278-7285 (1999)
   PUBMED   10066790
REFERENCE   9  (bases 1 to 6743)
  AUTHORS   Alecu,J., Schierbaum,L. and Ebrahimi-Fakhari,D.
  TITLE     AP-4-Associated Hereditary Spastic Paraplegia
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   30543385
REFERENCE   10 (bases 1 to 6743)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC130466.1, AK308644.1,
            CR749604.1, CR627314.1 and AI143020.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_007347.4.
            
            Summary: This gene encodes a member of the adaptor complexes large
            subunit protein family. These proteins are components of the
            heterotetrameric adaptor protein complexes, which play important
            roles in the secretory and endocytic pathways by mediating vesicle
            formation and sorting of integral membrane proteins. The encoded
            protein is a large subunit of adaptor protein complex-4, which is
            associated with both clathrin- and nonclathrin-coated vesicles.
            Disruption of this gene may be associated with cerebral palsy.
            Alternatively spliced transcript variants encoding multiple
            isoforms have been observed for this gene. [provided by RefSeq, Nov
            2011].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.18933.1,
                                           SRR1660803.65652.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261842.10/ ENSP00000261842.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3645              BC130466.1         11-3655
            3646-4276           AK308644.1         590-1220
            4277-5057           CR749604.1         4188-4968
            5058-6356           CR627314.1         487-1785
            6357-6743           AI143020.1         15-401              c
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q21.2"
     gene            1..6743
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /note="adaptor related protein complex 4 subunit epsilon
                     1"
                     /db_xref="GeneID:23431"
                     /db_xref="HGNC:HGNC:573"
                     /db_xref="MIM:607244"
     exon            1..246
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             97..3510
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     adaptor-related protein complex AP-4 epsilon;
                     epsilon-adaptin; AP-4 adaptor complex subunit epsilon;
                     adaptor related protein complex 4 epsilon 1 subunit"
                     /codon_start=1
                     /product="AP-4 complex subunit epsilon-1 isoform 1"
                     /protein_id="NP_031373.2"
                     /db_xref="CCDS:CCDS32240.1"
                     /db_xref="GeneID:23431"
                     /db_xref="HGNC:HGNC:573"
                     /db_xref="MIM:607244"
                     /translation="MSDIVEKTLTALPGLFLQNQPGGGPAAAKASFSSRLGSLVRGIT
                     ALTSKHEEEKLIQQELSSLKATVSAPTTTLKMMKECMVRLIYCEMLGYDASFGYIHAI
                     KLAQQGNLLEKRVGYLAVSLFLHESHELLLLLVNTVVKDLQSTNLVEVCMALTVVSQI
                     FPCEMIPAVLPLIEDKLQHSKEIVRRKAVLALYKFHLIAPNQVQHIHIKFRKALCDRD
                     VGVMAASLHIYLRMIKENSSGYKDLTGSFVTILKQVVGGKLPVEFNYHSVPAPWLQIQ
                     LLRILGLLGKDDQRTSELMYDVLDESLRRAELNHNVTYAILFECVHTVYSIYPKSELL
                     EKAAKCIGKFVLSPKINLKYLGLKALTYVIQQDPTLALQHQMTIIECLDHPDPIIKRE
                     TLELLYRITNAQNITVIVQKMLEYLHQSKEEYVIVNLVGKIAELAEKYAPDNAWFIQT
                     MNAVFSVGGDVMHPDIPNNFLRLLAEGFDDETEDQQLRLYAVQSYLTLLDMENVFYPQ
                     RFLQVMSWVLGEYSYLLDKETPEEVIAKLYKLLMNDSVSSETKAWLIAAVTKLTSQAH
                     SSNTVERLIHEFTISLDTCMRQHAFELKHLHENVELMKSLLPVDRSCEDLVVDASLSF
                     LDGFVAEGLSQGAAPYKPPHQRQEEKLSQEKVLNFEPYGLSFSSSGFTGRQSPAGISL
                     GSDVSGNSAETGLKETNSLKLEGIKKLWGKEGYLPKKESKTGDESGALPVPQESIMEN
                     VDQAITKKDQSQVLTQSKEEKEKQLLASSLFVGLGSESTINLLGKADTVSHKFRRKSK
                     VKEAKSGETTSTHNMTCSSFSSLSNVAYEDDYYSNTLHDTGDKELKKFSLTSELLDSE
                     SLTELPLVEKFSYCSLSTPSLFANNNMEIFHPPQSTAASVAKESSLASSFLEETTEYI
                     HSNAMEVCNNETISVSSYKIWKDDCLLMVWSVTNKSGLELKSADLEIFPAENFKVTEQ
                     PGCCLPVMEAESTKSFQYSVQIEKPFTEGNLTGFISYHMMDTHSAQLEFSVNLSLLDF
                     IRPLKISSDDFGKLWLSFANDVKQNVKMSESQAALPSALKTLQQKLRLHIIEIIGNEG
                     LLACQLLPSIPCLLHCRVHADVLALWFRSSCSTLPDYLLYQCQKVMEGS"
     exon            247..318
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            319..442
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            443..516
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            517..638
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            639..798
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            799..965
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1039
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1162
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1272
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1412
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1525
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1644
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..1947
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..2062
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2063..2186
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2187..2442
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2443..3000
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3001..3191
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3192..3349
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3350..6743
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6722..6727
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /note="hexamer: AATAAA"
     polyA_site      6743
                     /gene="AP4E1"
                     /gene_synonym="CPSQ4; SPG51; STUT1"
                     /note="major polyA site"
ORIGIN      
        1 agtgcctacg gaggccgggc cggcagcggc ggccgggcat gaagccgggc ggctacggga
       61 tcgcgggcgg cggcggcatc gcgggcggcg gcggcgatga gcgacatagt ggagaagacg
      121 ctgacggcgc tgccgggact ctttctgcag aaccagcccg gtggtgggcc cgcggccgcc
      181 aaggcgtcct tctcctcgag gctgggcagc cttgtccgcg gcatcacagc cctcacctcc
      241 aagcacgaag aagaaaaatt aatccagcag gaactgagta gtctgaaagc gactgtttct
      301 gctcctacta caacactgaa aatgatgaag gaatgtatgg tgagacttat atattgtgaa
      361 atgcttggat atgatgcttc ctttggctat attcatgcaa tcaagttagc ccaacaagga
      421 aacctcttag aaaaaagagt aggttatttg gctgtttcct tatttctaca tgaaagtcat
      481 gaattattgc ttctccttgt gaatacagtt gtaaaggatc tgcagagcac taacctagta
      541 gaagtgtgta tggcactgac tgttgttagc cagattttcc cctgcgaaat gattccagct
      601 gttcttccat taatagaaga taaacttcaa cattctaagg agattgtacg aagaaaagct
      661 gttctggcat tatacaaatt ccatctcatt gctcctaatc aagtacaaca tattcatatt
      721 aagtttcgga aagcactttg tgacagagat gttggggtca tggctgcctc cttgcatata
      781 tatcttagaa tgattaagga gaattcatct ggatataaag acttgactgg gagttttgta
      841 accattttga agcaagtagt tggaggaaag ctcccagtag aattcaatta ccacagtgtg
      901 ccagcaccat ggttacaaat tcagctcttg agaatactgg gacttctagg aaaagatgat
      961 caaaggacaa gtgaattaat gtatgatgtt cttgatgaat ccttacgaag agctgagtta
     1021 aatcacaatg tcacatatgc tattttgttt gaatgtgtgc atacagtcta ttctatttat
     1081 cctaaatcgg aattacttga gaaggctgcc aagtgcattg gaaaatttgt tctgtcacct
     1141 aaaataaatc taaaatattt aggactgaag gctcttacct atgttatcca acaggatcct
     1201 actctggctc ttcaacacca gatgacaata attgaatgtt tagatcatcc tgatcccatt
     1261 attaaaagag agactctgga acttctttac agaattacta atgcacagaa tataacagtt
     1321 attgtccaga aaatgcttga atatttacat cagagcaaag aagagtatgt catcgtcaat
     1381 ttggtcggca aaatagcaga gctggctgag aaatatgctc ctgataatgc atggtttatt
     1441 cagacaatga atgctgtgtt ttcagtagga ggagatgtaa tgcatcctga tattcccaat
     1501 aactttctga gactactagc ggaaggtttt gatgatgaaa cagaagatca gcaattaaga
     1561 ctctatgcag ttcagtctta tctcacttta ctggatatgg aaaatgtgtt ctatccacag
     1621 agatttcttc aagttatgag ttgggtatta ggggaatatt cctacctctt agataaggaa
     1681 acgccagagg aagttatagc taagctctac aagttactta tgaatgactc tgtgtcttca
     1741 gaaacaaaag cctggttaat tgctgctgtg accaaattga catctcaggc gcactcttct
     1801 aatacagttg agagattaat ccatgaattt accatatctt tggatacttg tatgagacaa
     1861 catgcatttg aattaaaaca tttgcatgag aatgtggaac ttatgaagag cttgcttcca
     1921 gttgacagga gttgtgaaga cttggtggta gatgcttctt tatcttttct ggatggtttt
     1981 gtggctgaag gactcagtca gggtgcagcg ccttacaaac ctccccatca acgccaggag
     2041 gaaaagcttt ctcaggaaaa agttctcaat tttgaaccat atggactctc cttttcttca
     2101 tctggcttca ctggacgaca gtctcctgct ggcatttctc ttggttcaga tgtatctggg
     2161 aatagtgctg agacaggact gaaagagaca aatagcttga agctggaagg tataaagaaa
     2221 ttgtggggga aagaaggcta tcttcccaag aaggaaagca aaactggtga tgaaagtgga
     2281 gctctgcctg ttcctcaaga gagtataatg gagaatgtag atcaagctat aactaaaaag
     2341 gatcaatctc aagttcttac ccaatctaaa gaggagaaag aaaagcagct gctggcatca
     2401 tcattatttg ttggtctagg atcagaaagt acaatcaacc tgctgggaaa agcagatact
     2461 gtctctcaca agttcagaag gaaatcaaaa gtcaaagaag ctaaaagtgg cgaaacaacc
     2521 agtactcata atatgacctg ttcttccttt agttctttgt caaatgtggc atatgaagat
     2581 gattattatt cgaatacttt gcacgataca ggagacaagg aattaaagaa attttctctc
     2641 acttcagaac ttttggattc tgagtcactc acagaactgc ccttggttga gaaattctca
     2701 tattgtagtc tgtctacacc ttcattgttt gctaataaca acatggaaat ttttcaccct
     2761 cctcaatcta ctgcagcctc agttgccaag gaaagctctt tagcttcatc ttttttggaa
     2821 gaaactactg aatacataca ctcaaatgct atggaagtct gtaataatga aactatatca
     2881 gtgtcttctt ataaaatttg gaaagatgat tgtttattga tggtctggtc agtcactaat
     2941 aagagtggtt tggaattgaa aagtgctgac ttagaaattt ttcctgcaga aaatttcaag
     3001 gtgactgagc aacctggatg ctgtttgcct gtaatggaag cagaaagcac caaaagcttt
     3061 caatatagtg tgcagataga aaaacctttt acagaaggaa atcttactgg ttttattagt
     3121 tatcatatga tggatactca ttctgctcag ctggaatttt ctgtaaactt atcactatta
     3181 gatttcatta gaccattaaa aatctcaagt gacgactttg ggaaactctg gttatccttc
     3241 gcaaatgatg tgaaacaaaa tgtaaaaatg tcagaatctc aagctgcact tccttctgca
     3301 ctaaagactc tgcaacagaa actaagactc catattattg agattatagg caatgaaggg
     3361 ctattggcct gtcagctgct cccatccatc ccctgcttac tgcattgccg agttcatgca
     3421 gatgtattag ccctgtggtt cagatcctcc tgttctactc ttcctgacta tttactgtat
     3481 cagtgtcaaa aggtgatgga gggatcctag cagaagccct gctaaatttt actccatcaa
     3541 gatcaatggt ttacatagat aaacttattt accaaagtaa aaagaactca tggtacttct
     3601 aatgaaaatg gggattatta caagtgtggt ttatatgttt tctttgtgat tcctggtcaa
     3661 gaaagatccc caaaactgta tccctaacct ttaactcagg attgtacagt atgtttaggt
     3721 ccctcaaaaa gtgacctaag ctaatgttat aaactgctaa tgatttatat atcacttagt
     3781 gtgtagaggg actgaaaata tttattttgt tataaataat tttatagcac gtttactcta
     3841 gtgctagcta atttgtaata aagccaagtc tcagttttct gcattaaatg gaagggagac
     3901 atgaaattga taatctcaaa cttaattcat atttggcttt ggaatgtagt gtatggtttt
     3961 tggtagggaa gtcaatattt tcgattatgt tttgcttaga tcagtgttga actaagttgg
     4021 catagcacac actaacagtt gtaggagatc catgagcatg ctggatattg aggggatcca
     4081 atctgtgatc tattttttat tatagataac tgttacacat aatttcagat cgtttggctg
     4141 taaaatttgt ctgttttcca cttggagtta ttttaaaatt caaagtaaat cactctattc
     4201 ttattttgaa aactcagagc cattttatat agaaacaaaa cccataagag cacacagtaa
     4261 caaagcaaac agatacaaaa atgacttttt agttatgaca aatattttag ttggttacta
     4321 cttaggtcct tttggcaaat gaaaatttgt gattagagta taaatggcaa aactatcttt
     4381 tcctggcctt tgaatccact agtacttaat ggatatagct gaagcagaga ctaagtataa
     4441 catacaggtt cttcagtaaa atatatcatt ttcatgttat agttatgcct gtgtggcctc
     4501 tgtcagaact attatcagta aaagaggtta agcctatata tgaatgatgg accaaaacct
     4561 tgaggttgta cattaactga aatagtattt tataaaatct tatggtgctg tggaaaatat
     4621 ttattatttc accttttctg acacttcacc atggagacta ttcaggtaat acaattttaa
     4681 gtcattgcag ttaatactct ggttaattgg tactgttaat cacccaggtc aggaattctg
     4741 attttaggtt atctcattaa tcatggctga taaagaagct aaggggattc tccagatcta
     4801 atagatttca ttagtcattt gtgtcgagta ttcctcccta aaaacaaaag cagtattttc
     4861 tttctcaatt aatttttaag ttattcatta aaaaatatat aacatcttaa ttttattctt
     4921 ttaaaaacat acagtgaagc cttgctacaa gagtcataag tgactggaat ttcttgcata
     4981 ataggttcct ccttttgaat agatcagatt catccccagg ctctcagcag tgtcatgatg
     5041 aaatgagtgt gctgacttca ggggtgtggt ggacactggt agctagcctt atcagctccc
     5101 tccttattcc tacctctgtc agcagcatgc ctcgcccctt acctaattct gctgtgactc
     5161 tgacagagag gaagtgcctt ctcactttgc aaaggaatgc agcatgtcac aaaggaggtg
     5221 ctccaatttt ttgtgtcctt acttccctat gtttgcctag ttactgaggc taggtcgagt
     5281 gtgaagcaat aggtgtaaga tgctgagagc acatggacag ctaccaggga gatctgttgc
     5341 ttggttacag ggtgtattca tgagtggtag ctgcagttgt cgtgagagac atgcttggca
     5401 tacagagctg tctaatgggt gggagacatg taatggtaga tttagggctg ggagcatgat
     5461 tttgggtctg cccaaaggaa catggtgtca cttttgttct ttctgaccca ctaaataaaa
     5521 agtagtgtaa cagggtttca ctggaatgtt tattagaaag tttacacttt ttatattttt
     5581 atgattttaa aaacaaacaa taatagaaaa acagatttta aggaacttca gagcagtata
     5641 tattctgtct aaactcactg aatattttaa gttgtaaatc aggtgtccac caacctttat
     5701 ttttgcattg caggagatgt gactgtaaag tagggagttt ttttttttga gacagagttt
     5761 cacccttgtt gcccaggcta gagtgtaatg gtgcaatctc cgctcactgc aacctctgcc
     5821 tcccaggttc aagcgattct cctgcctcag cctccctagt agctgggatt acaggcatgc
     5881 accaccacac ccggctaatt tttttatatt tttagtagag acaaggtttc tccatgttgg
     5941 tcaggctggt ctcgaactcc cgacctcagg tgatctgccc gcctcagcct cccaaagtgc
     6001 tgggattaca ggcgtgagcc actgcgcccg gccaaattat ttttgtgggt agattcattc
     6061 tctttaccta atagtcataa tttagtcagt gaagggacaa catctggggt aagagctatg
     6121 gaggaggtgc tcagctttgt gagttttgtg ccaggcatct ttgctgaaaa tggagatggc
     6181 agcaagtgtg aatctggggg aatataaggg aagacagaaa agccttaaga gtagacggag
     6241 gaagaagggt accagaatag tccctgtgga ggcaggcaga tcagagctca gggtttgaaa
     6301 gtgggcctga gtaagggtcc tgctagagaa actgcagatg gaagctgagc agttaccaac
     6361 tgctaatgtg ccaagtatac caagcacagc atgtgtcctg tagaggagca ttattttaaa
     6421 caatgttcta ctatcatcta ccaaaataat tgtggtaaaa gtgtagtatt ttattttttc
     6481 cttttatgtc agcaatattt taaaatattg atctgtttat cttatatttt tatggataaa
     6541 tgtagtattt gcctcatgat tgtgagccac tgagagtgca ctgaacctat tcacatgtct
     6601 ttaagatgtt ttgctgtgtt aaaattttag gagagaaaaa tacaagtgaa taattattta
     6661 ataggcattt attactttat tttggagttg aattctgtca tttgaaaaag ttcagtaatg
     6721 taataaatac aaagtgatct gca
//
