LOCUS       NM_032493              12859 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens adaptor related protein complex 1 subunit mu 1
            (AP1M1), transcript variant 2, mRNA.
ACCESSION   NM_032493
VERSION     NM_032493.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12859)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 12859)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 12859)
  AUTHORS   Singh,R., Stoneham,C., Lim,C., Jia,X., Guenaga,J., Wyatt,R.,
            Wertheim,J.O., Xiong,Y. and Guatelli,J.
  TITLE     Phosphoserine acidic cluster motifs bind distinct basic regions on
            the mu subunits of clathrin adaptor protein complexes
  JOURNAL   J Biol Chem 293 (40), 15678-15690 (2018)
   PUBMED   30135209
  REMARK    GeneRIF: It has been shown that the cytoplasmic domains of furin
            bind the mu subunits of AP-1 and AP-2 in a
            phosphorylation-dependent manner.
REFERENCE   4  (bases 1 to 12859)
  AUTHORS   Lebrun,M., Lambert,J., Riva,L., Thelen,N., Rambout,X., Blondeau,C.,
            Thiry,M., Snoeck,R., Twizere,J.C., Dequiedt,F., Andrei,G. and
            Sadzot-Delvaux,C.
  TITLE     Varicella-Zoster Virus ORF9p Binding to Cellular Adaptor Protein
            Complex 1 Is Important for Viral Infectivity
  JOURNAL   J Virol 92 (15), e00295-18 (2018)
   PUBMED   29793951
  REMARK    GeneRIF: found 31 ORF9p interaction partners, among which was
            AP1M1, the mu subunit of the adaptor protein complex 1 (AP-1). AP-1
            is a heterotetramer involved in intracellular vesicle-mediated
            transport and regulates the shuttling of cargo proteins between
            endosomes and the trans-Golgi network via clathrin-coated vesicles.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 12859)
  AUTHORS   Xiao,F., Wang,S., Barouch-Bentov,R., Neveu,G., Pu,S., Beer,M.,
            Schor,S., Kumar,S., Nicolaescu,V., Lindenbach,B.D., Randall,G. and
            Einav,S.
  TITLE     Interactions between the Hepatitis C Virus Nonstructural 2 Protein
            and Host Adaptor Proteins 1 and 4 Orchestrate Virus Release
  JOURNAL   mBio 9 (2), e02233-17 (2018)
   PUBMED   29535204
  REMARK    GeneRIF: Here, the authors demonstrate that dileucine motifs in the
            hepatitis C virus NS2 protein mediate AP-1A, AP-1B, and AP-4
            binding and cell-free virus release. Moreover, they reveal that
            AP-4, an adaptor not previously implicated in viral infections,
            mediates cell-to-cell spread and hepatitis C virus trafficking.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 12859)
  AUTHORS   Peyrard,M., Parveneh,S., Lagercrantz,S., Ekman,M., Fransson,I.,
            Sahlen,S. and Dumanski,J.P.
  TITLE     Cloning, expression pattern, and chromosomal assignment to 16q23 of
            the human gamma-adaptin gene (ADTG)
  JOURNAL   Genomics 50 (2), 275-280 (1998)
   PUBMED   9653655
REFERENCE   7  (bases 1 to 12859)
  AUTHORS   Aguilar,R.C., Ohno,H., Roche,K.W. and Bonifacino,J.S.
  TITLE     Functional domain mapping of the clathrin-associated adaptor medium
            chains mu1 and mu2
  JOURNAL   J Biol Chem 272 (43), 27160-27166 (1997)
   PUBMED   9341158
REFERENCE   8  (bases 1 to 12859)
  AUTHORS   Seaman,M.N., Sowerby,P.J. and Robinson,M.S.
  TITLE     Cytosolic and membrane-associated proteins involved in the
            recruitment of AP-1 adaptors onto the trans-Golgi network
  JOURNAL   J Biol Chem 271 (41), 25446-25451 (1996)
   PUBMED   8810314
REFERENCE   9  (bases 1 to 12859)
  AUTHORS   Page,L.J. and Robinson,M.S.
  TITLE     Targeting signals and subunit interactions in coated vesicle
            adaptor complexes
  JOURNAL   J Cell Biol 131 (3), 619-630 (1995)
   PUBMED   7593184
REFERENCE   10 (bases 1 to 12859)
  AUTHORS   Ohno,H., Stewart,J., Fournier,M.C., Bosshart,H., Rhee,I.,
            Miyatake,S., Saito,T., Gallusser,A., Kirchhausen,T. and
            Bonifacino,J.S.
  TITLE     Interaction of tyrosine-based sorting signals with
            clathrin-associated proteins
  JOURNAL   Science 269 (5232), 1872-1875 (1995)
   PUBMED   7569928
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC386056.1, BC017469.2,
            AK027528.1, BU729646.1, BQ014582.1 and AC020911.9.
            
            On Jun 2, 2019 this sequence version replaced NM_032493.3.
            
            Summary: The protein encoded by this gene is the medium chain of
            the trans-Golgi network clathrin-associated protein complex AP-1.
            The other components of this complex are beta-prime-adaptin,
            gamma-adaptin, and the small chain AP1S1. This complex is located
            at the Golgi vesicle and links clathrin to receptors in coated
            vesicles. These vesicles are involved in endocytosis and Golgi
            processing. Alternatively spliced transcript variants encoding
            distinct protein isoforms have been found for this gene. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) lacks a coding exon in the
            middle region, as compared to variant 1. The reading frame is not
            affected and the resulting isoform (2) lacks an internal segment,
            as compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.577764.1, BC017469.2
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000291439.8/ ENSP00000291439.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                DC386056.1         58-67
            11-1138             BC017469.2         13-1140
            1139-1963           AK027528.1         1104-1928
            1964-2245           BU729646.1         69-350              c
            2246-2298           BQ014582.1         19-71               c
            2299-12859          AC020911.9         115307-125867
FEATURES             Location/Qualifiers
     source          1..12859
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.11"
     gene            1..12859
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /note="adaptor related protein complex 1 subunit mu 1"
                     /db_xref="GeneID:8907"
                     /db_xref="HGNC:HGNC:13667"
                     /db_xref="MIM:603535"
     exon            1..158
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..1388
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /note="isoform 2 is encoded by transcript variant 2;
                     clathrin assembly protein complex 1, medium chain;
                     clathrin coat assembly protein AP47; golgi adaptor AP-1 47
                     kDa protein; HA1 47 kDa subunit; clathrin assembly protein
                     complex AP1, mu subunit; clathrin adaptor protein AP47;
                     AP-1 complex subunit mu-1; mu-adaptin 1; mu1A-adaptin;
                     AP-mu chain family member mu1A; clathrin coat-associated
                     protein AP47; adaptor protein complex AP-1 mu-1 subunit;
                     golgi adaptor HA1/AP1 adaptin mu-1 subunit; clathrin
                     assembly protein complex 1 medium chain 1; adaptor protein
                     complex AP-1 subunit mu-1; adapter-related protein complex
                     1 subunit mu-1; clathrin assembly protein complex 1 mu-1
                     medium chain 1; adaptor related protein complex 1 mu 1
                     subunit"
                     /codon_start=1
                     /product="AP-1 complex subunit mu-1 isoform 2"
                     /protein_id="NP_115882.1"
                     /db_xref="CCDS:CCDS12342.1"
                     /db_xref="GeneID:8907"
                     /db_xref="HGNC:HGNC:13667"
                     /db_xref="MIM:603535"
                     /translation="MSASAVYVLDLKGKVLICRNYRGDVDMSEVEHFMPILMEKEEEG
                     MLSPILAHGGVRFMWIKHNNLYLVATSKKNACVSLVFSFLYKVVQVFSEYFKELEEES
                     IRDNFVIIYELLDELMDFGYPQTTDSKILQEYITQEGHKLETGAPRPPATVTNAVSWR
                     SEGIKYRKNEVFLDVIESVNLLVSANGNVLRSEIVGSIKMRVFLSGMPELRLGLNDKV
                     LFDNTGRGKSKSVELEDVKFHQCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPL
                     IWIESVIEKHSHSRIEYMIKAKSQFKRRSTANNVEIHIPVPNDADSPKFKTTVGSVKW
                     VPENSEIVWSIKSFPGGKEYLMRAHFGLPSVEAEDKEGKPPISVKFEIPYFTTSGIQV
                     RYLKIIEKSGYQALPWVRYITQNGDYQLRTQ"
     exon            159..315
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /inference="alignment:Splign:2.1.0"
     exon            316..383
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /inference="alignment:Splign:2.1.0"
     exon            384..514
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /inference="alignment:Splign:2.1.0"
     exon            515..662
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /inference="alignment:Splign:2.1.0"
     exon            663..789
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /inference="alignment:Splign:2.1.0"
     exon            790..932
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1004
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1163
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1289
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1365
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..12859
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2274..2279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /note="hexamer: ATTAAA"
     polyA_site      2298
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /note="major polyA site"
     regulatory      12840..12845
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
                     /note="hexamer: AATAAT"
     polyA_site      12859
                     /gene="AP1M1"
                     /gene_synonym="AP47; CLAPM2; CLTNM; MU-1A; mu1A"
ORIGIN      
        1 ggaagtggag gtgagctgtc gcgggcggcg cccggccttg ctcaacgccc agcagtcccc
       61 accgtcgctg ccgccgccac cgccctcggc cgctgccgag gcctcctgca gccatcatgt
      121 ccgccagcgc cgtctacgtg ctggacctga agggcaaggt gctcatctgc cggaactacc
      181 gtggcgacgt ggacatgtca gaggtggagc acttcatgcc catcctgatg gagaaggagg
      241 aggaggggat gctgtcgccc atcctggccc acgggggggt ccgtttcatg tggatcaaac
      301 acaacaacct gtatctggtt gccacatcca agaagaacgc gtgcgtgtcg ctggtctttt
      361 ctttcctcta taaggtggtg caggtgtttt ccgagtactt caaggagctg gaggaggaga
      421 gcatccggga caactttgtt atcatctacg agctgctgga cgagctcatg gacttcggct
      481 acccccagac caccgacagc aagatcctgc aggagtacat cactcaggaa ggccacaagc
      541 tggaaacagg ggccccgcgg ccaccagcca ccgtcaccaa cgcggtgtcc tggcggtccg
      601 aaggcatcaa gtatcggaag aatgaggtgt tcttggacgt catcgagtct gtcaacctct
      661 tggtcagcgc caacggcaat gtcctgcgca gcgagatcgt gggctccatc aagatgcgag
      721 tcttcctctc gggcatgccc gagctgcgcc tgggcctcaa cgacaaggtc ctctttgaca
      781 acacgggccg cggcaaaagc aaatccgtgg agctggagga tgtgaagttc caccagtgtg
      841 tgcggctatc acgcttcgag aatgaccgca ccatctcctt catcccaccc gacggcgagt
      901 tcgagctcat gtcctaccgt ctcaacaccc acgtcaagcc tttgatatgg atcgagtcgg
      961 tgatcgagaa gcactcccac agccgcatcg agtacatgat caaggccaaa agccagttca
     1021 agcggcggtc aacagccaac aacgtggaga tccacattcc cgtgcccaat gatgccgact
     1081 cacccaagtt caagacgacg gtggggagcg ttaagtgggt ccccgagaac agcgagatcg
     1141 tgtggtccat caagtccttc ccgggcggca aggagtacct gatgcgggcc cacttcggcc
     1201 tgcctagtgt ggaggccgaa gacaaggagg gcaagccccc gatcagtgtc aagttcgaga
     1261 tcccttactt cactacctcc ggcatccagg tgcgctacct gaagatcatt gagaagagtg
     1321 ggtaccaggc cctgccctgg gtgcgttata tcacgcagaa tggagattac cagctccgga
     1381 cccagtgagg ggctgtcgca gccaacaccc cggcctcggg gctcctggtg gcagcaccag
     1441 gggacacacc tgccaaaccc accagatgga ggggccctcc ctggtctctg gccaccctcc
     1501 cagcctctgc ccagggaccc ctgccttccc caggccatct gctctgccgt cgacactcgt
     1561 ctcagaagcc cctttcccag aagaggctgg tcttcaagaa gtctcgtttc tttgcccctg
     1621 aagtcagttt caggggaagg atgtgaaatt tttccgtgta gaggttacag ccttttatgc
     1681 tgttgagctc ccaggtacca aaaagcttgg ccaacgcttg ccagccagcc agctgcaggt
     1741 ggcatctgcc acgaaggaag cgccagcctc gccaggccag caggggcgtc gttttgttgc
     1801 cattttgttg aacgttatgg gtttatgggt gttcctggaa cttgtctttg tgcattcgtt
     1861 gctgtttgtg ttaccctcac tgtccccatg tcccacccac gtcctacggc actcaggaag
     1921 cacttggtga ggacgagccc tcacccttct tgtcttcctt cccggcagcg cccgcagcgg
     1981 gccatttaca cgtcgaggct ggcacctggc gcgctcgggg gccactgtag cgtctgcctg
     2041 ctccctggac tcgcaggcct cgcctgtggc gccttcccag ggccagcctg ggtcacgaga
     2101 tgctgtcact cagccagatc agtattgacc caccagggga ggtggggttt ggtgagagac
     2161 gccagcctca gactttttcc cactgagggt ccagagagcg gggccacgtg tcacccacgt
     2221 ctgcgcttgg tcacccgtcc tccccaccct gtgtgtgttt atgtcatagt tacattaaat
     2281 tccattcatt gaatactcca cagccttcct ctgatgcagc gtggtcccaa ctcctcctgc
     2341 gtgaccacag ggcttgcctg gttttgtcgc cgaccttccc tgtggcccca cgaggtgtca
     2401 ccagctccct ctgcttcccc agctgaaaca caggaaaagc agctgcggga agaggcagct
     2461 gcagtgctgg accatgggcc accactcctg ggccacacag aggaggccac agaggcagag
     2521 tcttctctca tccaatctgc tgcaccctca gatggccaac agcttgtccc cacctctccc
     2581 caaactgatg gcccactcag agcatggtcc ttgtgtccac agcagcatcc cctgtgtcct
     2641 agctagaaat gcaggttcgt gggccagccc tgacctgcca aagtggaggc tctggggcca
     2701 gggtcccgga aaacattaac aaggccccca gatgcttctt ctgtgaactg ctacttggtc
     2761 catgggggaa atagcacata tgcaggaagt gctgcctgct cttggccccc tccccgacca
     2821 gtgctgatgg ccctgcctgg ccagcaccct cttgccttca ttctccccct ccctctcctg
     2881 tcttgccgag ctcgtcctca ggtgtctagt gggcatccga cctaacagcc tgagtagaac
     2941 cttccgccca cgtaggtctt ccccatctca gtcaatgacg ctgccccagc ccaggcctca
     3001 gaccaaagcc ctgaaggcat ccctggggcc cctcccctca ctgtccacac ccatatcagc
     3061 agccagccct catggcccct ccagaaaacc ctggggctga ccccatttgc catcctcgct
     3121 gccacccccg gggcctagct ccagtgtggg ctgtcactgt ccccaccctg tggagccccc
     3181 atgctgggac accgcaggga caggagaggg ctggtccaca ggacagaaga gagacttcag
     3241 tttccagcaa cttatcagat aagacctgca aaccctccac tacagactgt agaaatgcag
     3301 gaaatgaagc agacgcacct agccgtgccc ataaaaaagc aaggagaccc acaggagccc
     3361 aagacgaagg aagtagacgc ggaagacacc gtgggggctc acaccggagt tgctcttgga
     3421 aaccaccggg tttaggtttg ggggccaggt ggcagatagg actttgggca tcgggaagat
     3481 ggtgaaatgc agtggagatg actcctgcgt gaagctggga tcctcgaggg tcatgatact
     3541 taacagtaaa aagatggact agaaaaaaca tttgccctcc acccctgaga ataagcactg
     3601 tgtcctctct ctgcctgaga tccacaggag aaaaagtcac cactcattta gaccccacgc
     3661 ttgtaatatc aatctggttt gcagacaacc aggccaagat gtttatgaca gaaaacctcc
     3721 ctggagggac ctaacttcat gccttgttac ctaagatcct cgcacacaat agaccctcaa
     3781 catgagggca catcctgaaa attacaaaac acgtgcatga aagcaaactt gaaacagcag
     3841 gatcagatcc tcacgaactt caaagattat cggcatgtaa acaaatacac tatgcagtca
     3901 atctgttgca agtgcctaaa ggcacagaga ctctgaatag aaaagagcaa aatgctgtta
     3961 agacagagct gttacaacat ccaacaaggc aaataatttg catgcgggtt ataacgtcta
     4021 caaatcagaa aaagagaacc taacaaaatg ggctgtgtgt gaacagacag tatgaagaag
     4081 atgaaataca aatggcgacc gttcggccag gcgtggtggc tcacgcccgt gagcccaata
     4141 ctttgggaag ccgaggcaga ccgatcactg gaggtcagga gttcgagacc agcatggcca
     4201 gcatggtgaa gccccatcta tactaaaaat acaagaatta gccgggtgtg gtggtgcgtg
     4261 cctgtaatcc cagctactca gtatgctgag gcaggagaat ggcttgaacc caggaggtgg
     4321 aggttgcagt gagccaagat agtgttactg cactccagcc tgggcaacag agcgagactc
     4381 cgtctcaaaa cacaacaaaa accggcctag cgcagtggct tatgcctgta atcccagcac
     4441 tttgggaggc tgagacgggt ggatcacctg aggtcaggag tccgagacca acctggccaa
     4501 catggcgaga ccccatctct actaaaaata ccaaaattag ccaggcatgg tggcgggcgc
     4561 ctgtaatccc agctacttgg gaggctgagg catcagaatc atttgaaccc caggaggcag
     4621 aggttgcagt gagccgagat cataccacct gcactccagc ctgggcaaca gagcaagact
     4681 tcatctcaaa agaaaaaaaa aaggcaaacg ttcaaccatg tagctattca ggaaatatgt
     4741 acacataaac caagaccaca gttagatgct atttgcatca tgtcggcccc ccccaaaaaa
     4801 attttttttt ttgagacagg gtcttgctat gttacccagg ctggagtgca gtggcgtgat
     4861 cacggctcac tgcagcctcg agctgccagg ctcagccaat cctgcctcag cctcttgagg
     4921 agctgggact acaagcacat gccaccatgc tgggccaatt tttcatattt tttatagaga
     4981 tggggtctcg tcattttgtc cacactggtc tccaactcct gggcttgagc catcatcctg
     5041 ccttggcctc ccaaagtgct gggattacag gcgtgagcca cctcgccacg cctcctgaac
     5101 atctagaacg tgctagctat gtactgactg tgggaaagtt gagaacatcc tccctgaagt
     5161 catctttatg ccagaattct cttgcagaac tctagttgag aaaagccctg agaagtccat
     5221 gcaccaatat atagagaaat atcaggtgta cagttacact tacagcagca tagttcatga
     5281 tagcaggaaa ttggaaagca accaaaagtc cttcaatagg aaaagggacc actgtagtcc
     5341 attcatgcaa gagattacag cagtgaaaat gaattcaact agagcagtga tatggctcag
     5401 aatacaactt aaacaacatt gagcgggaaa aatgtttaca aaagaaaatg cagtaatcta
     5461 gtataaaata cggaaataat attttgcgtg cttagggatc tatatatatc cagcaaaagt
     5521 ctaacgagat gcttgagaat ttgaaaaaat tcacatccct ggttatctct gaaaggcaaa
     5581 aacaggaagg gggtatgact ggggcctctg ctgtgttggc aatttcctta agcttggtat
     5641 tgcttagtgg cttgttctgt attctttctt tatgcttttt tttttttttt ttgacagggt
     5701 gtcactctgt tgcccaggcc aaagtgcagt ggcatggtca cggctcactg cagcttcaaa
     5761 ctcctgggct caagtgatcc tcccaccttg gcctcccaaa gtgctgggat tacaggcatg
     5821 agccacagtg cccagccctt tttgtagttc ttaatatact ttttgtagtt tttttgtttt
     5881 ttgtttttgt ttttgttttt tttgagacag agttttgctc ttgttgccca ggctggagtg
     5941 caatggtgtg atctcggctc actgcaacct ccgccttctg ggttcaagca attctcctgc
     6001 ctcatcctcc tgagtagctg agattacagg catgcgccac catgcctggc taattttgta
     6061 tttttggtag agacgggatt tctccatgtt ggtcaggctg gtctcgaact cccggcttca
     6121 ggtgatccgc ccccctcacc ttcccaaagt gctgggatta caggcatgag ccaccgcgcc
     6181 cggccagttc tctttttttt tttttttttt tttttttttt tttttttttt ttaagactgc
     6241 aaagctgggc atggtagtat gcacctgtag tcccaactac ttgggaggct gaggctagag
     6301 gattacttga gcccaggagt ttaaggacag cctgggcaac atagtgagac cctgtctcta
     6361 aaacaaacca acaaaacacc ccccaaaaat gacaatgcag atttggaaga aaaaaataga
     6421 ttaaaaatgt agtcatagga attaacaact tggggccggg catggtggct cacgcctata
     6481 atcccagcac tttgggaggc tgaggcggcc agatcatttt aggtcaggag tttgagacca
     6541 gcctggccaa catggtaaaa cctgattgct actaaaaata caaaagaatt agctgggcgt
     6601 agtggctcat acctgtaatc ccagctactc aggtggctgg ggcaggagat tcgcttgaac
     6661 ccgggagatg gcgctcgcag tgagtcaaga tggcaccgtt gtactcttgc ctgagcgaca
     6721 cagtgagaca ctgtctcaaa aaaaaaaatt aacaacttgg ttggagggtg ttaaaataca
     6781 agtgagacat agatgaagag aattagtgca ctagaagaga gctgtctgag gcagttaccc
     6841 gggacatagc acagctaatg agacatatat atatttacag tcaaccctcc acatccatgg
     6901 gttctgcatc catggattca accaagagtg agttgaaaat atttgaaaaa caattgtgtc
     6961 tgtgctggac atgtacaggt tttctttttt attgtcatta ttccttaaat aatacagtat
     7021 aatgattatg tagcatctac attgtattag gtaatctaga gatgatttaa agatgatgtg
     7081 cataggatat atgcaaatat tgtgccattt tatataaaag acttgagcat cctcagattt
     7141 tggtatccaa ggggagtcct ggaagcaatc ccttgaggat atggagggac gactctgtgt
     7201 gtgtgtgtgt gtgtgtgtgt gtgtgtatgt gtgtgtgtgt gtgtgtgtgt gtgtgatgtg
     7261 cgtgtgtggt tagcacaaga caataagaag gcccaacatg ccagcaagta tctaacacaa
     7321 accaaaaaat gagaactggg gagcagccac atgcaaagag atcgtggcca gaattttcca
     7381 aaattaatga aaatcttgaa gtgttttttg tttttcattt tgacggagtt tcgcgtttgt
     7441 tgcccaggct ggagtgcaat ggcgaaatct cggctcatta caacctctgc ctcccaggtt
     7501 caagcaattc tcatatctca gcctcccaag cagctgggat tacaggtgca caccaccacg
     7561 cccggctaat ttttgtattt tttgtagaga tggggtttca ctatgttggt caagatggtc
     7621 tcgaactcct gacctcaggt gatctgcctg cttcggcctc ccaaagtgct gggattacag
     7681 gcatgagcca ctgcgcccag cctgaaaatc ttgaaatttt aagatccagg aaaccgttga
     7741 gtccaaagca tgatacacaa acctaaattc gtaactaaaa taatcatagt cgaaccacag
     7801 aacatatcag actcagggaa gatcctaaca gcaacactaa gagattaaaa tgtctctcca
     7861 aagagcaatt ggacagagaa ctggtttcac aactggaaca acaaaagtcc gaagatagag
     7921 catggcttca aattactgag agaagtgcag tctagaattc tgagcccacc tcatctgtaa
     7981 aaggtaggac aagccgggca tggtggctcc cgcctgtaat cccagcactc tgggaggccg
     8041 aggcgggcag aggtcaggag tttgagacca gccggaccaa caaggagaaa ccccatctct
     8101 actagaaata caaaattagc caggtgtggt ggcgggcacc tgtgttccca gctacttggg
     8161 agactgaggc agacagaatt gcttgaacca gggagacaga ggttgcagtg agccgagatc
     8221 gcgccactgt actccagctt gggcaaagtg agattctgtc tcaaaaaaaa aaaaagacaa
     8281 gataaaaaca atttatcaat aacagattcc ccgaaaaaag gaacttgtgt acataaggaa
     8341 aggaagaaag atgtgagaag gaatgccgga aaaccttaga aaccattgga tctgtctaaa
     8401 ctgtcattga ctgtgtaaag caataataac agtgactaac gtatagagta gcgacaaaag
     8461 gcgcaactga agtactagac aacatcctgg tgttcatgcc tttcaaggtc actatgctat
     8521 ttgggaggag attcggctaa agtccacgag ggccacgtat ttatatataa tttctagacc
     8581 agtggttgga aaagggttga aagaaaactt ttaacagatt aataggaaga aggaaaagag
     8641 aaggaggcgt agaaaaatca ggaggcactg ggcgcggtgg ctcacgcctg taatcccagc
     8701 actttgggag gctgaggcag gcggatcacc tgaggttggg agttcgagat cagcctgact
     8761 aacatgagaa accccgtctc tactaaaaat ataaaattag ccgggtgtgg tggtgcatgc
     8821 ctataatccc agctactcgg gagggtgagg caggagaatc gcttgaacct gggaggcaga
     8881 ggttgtggtg agctgagatc gcgccattgc actccagcct gggcaatgag cgaaactcca
     8941 tctcaaaaaa aaaaagaaag aaaaagaaaa agaacaatca ggaggccagg ttcagtggct
     9001 catgcctgta atcccagtgc tttgggaggc tgaggctgag acaggtgaat tgcttgagcc
     9061 taggagtttg agaccagcct gggcaacatg gagaaactct gtctctacaa aaattagcca
     9121 ggcatggtgg cgcacacctg tagtccaaac tactcaggag gctgagttgg gaggatcacc
     9181 tgagttcagg aagctgaggc tgcagtgagc tgtgattgca ccactgcact ccaggttggg
     9241 tgacagagtg acaccctgcc tcaaaaaaaa aaaataaaat aaaggaaaaa tcaggataaa
     9301 tgcagggatg ttctaaactt tttaaaataa taaataatag tacttaaaac tgcactggga
     9361 cttttgggac acccctagca tctctggctg cagaaagcat aaaataagat tgtagaaata
     9421 aatccagata ttaggaacca caacaggtgt aaatgaagca aagctgctag ttgaagatgt
     9481 tgtcggattg aactgaaata caaaatcctg tgatatcctg tttatacgcg atacatctaa
     9541 aatgtaagta cacaacacaa ttgaaagtaa aaaaacatta aaagatgtga cacttaaaat
     9601 ataagcacac aacacagtta aaagtaaaaa gacattaaaa gatgccaaaa gaaatctcat
     9661 atggttatat taattccaga cagaatagat gtatttattt atttattcat tcatttaatt
     9721 ttttgagatg gagtctcgct ctgtcgccca ggctggagtg cagtggcgcg atctttgctt
     9781 actgcaacct ccgcctcccg ggttcaagtg attctcctgc ctcagtctcc tcagtagctg
     9841 ggattacagg catgcaccac catgcttggc taaatttttt tatttttgta ttttttttat
     9901 ttttagtaga gacagggttt tgccatgttg gccaggctgg tcttgaaccc tgacctcagg
     9961 tgatccaccc ccactggcct cccaaagtgc tagtggcagg tgtgagccac cacacacggc
    10021 cagaatagac tttaaagcaa gaaagcaaaa gaaaaaatgt attagatccc cgagaagttt
    10081 ccacacaact ccaagaatta ttattagagt tcccactctc tgaggaattt tcaagagaaa
    10141 ttagttagaa ttcaacaaga aataaattac tttaaaatat ttccaaatat gagaggtttt
    10201 ttttgttttt gtttttgttt ttgtttttgt ttttgttttg agaaagggtc tctgttgccc
    10261 aggctggagt gcagtcagtg gcacgatgtc agctcactgc aaccttggcc tcctaggctg
    10321 aaatagtcct cccatctcag tctcacaagt agctgggatt acaggtgagc accaccaagc
    10381 tcagctattt tttttttttt tttgtatttt tagtagagat ggagttttgc catgttgccc
    10441 aggctggtct tgcacttggg ctcaagtgat ccacctgcct cagcctccac agtgctggaa
    10501 ttacaggtgt gagtcaccat gcccagccaa tatgagagat tttaaagcac atttttcaat
    10561 caccatgtgt caaagaagaa aatataatgg aagttagaaa atattagaga atggccaggt
    10621 gcagtggctc atgtctataa tcccagcact ttaggaggct gaagcaggtg aatcacttga
    10681 gctcaggagt tcgagaccag cctggccaac atggcgaaaa tccatctcta ctaaaaatac
    10741 aaaaattaac caggtgtagt ggcacacgcc tgtaaatccc agctactcag gaggctgagg
    10801 cccaagaatg gcttgaaccc tggaggcaga ggttgcagtg agctgagatt gtgccactgc
    10861 actccagcct gggcaacgaa acgagagtct gtctccaaat aaataattag agaatatttt
    10921 tcaatctgga tgcaggtgac atgggtgtgc tctcgagaat ccatcaagca ttgatgattt
    10981 gcacactctt ttgtgtatat gttacacttc agcaaaaaca ttcacaaagg ggaaaaaggc
    11041 aaaagatact taaagcagcc agaggtaaag acacatcacc ttcagacatg acaaccagac
    11101 agacagacag ctggcttttc aaaggaaccc agaaaacagt tgggtgacat tttcaacatg
    11161 ctaaaagaaa accactaacc caaatcagag acccagcaaa agtatttcaa gaatgaggtt
    11221 acagtaaaaa cattttgcag acaaataacg aaagagtttg ccagcagcag gctaacacta
    11281 aagaaaattg taaacagtgt actcacatca ggcacaagga aagtgatctc agacggaagg
    11341 gcatgatgca agaagaaata aaagcaaaga agtagaaaat acatgagaaa atctagacaa
    11401 agattgcata aacagtttct ggagtttttt taagtgatag aattatacaa caggaaaaaa
    11461 agtctgcagg gagtaaactg agttaaaatg ttccaagtta atgactgtct gggaggagaa
    11521 taaataacat ggatacactt tgggaggccg aggcgggcgg atcacgaggt caggagatcg
    11581 agaccatcct ggctaacacg gtgaaacccc gtctctacta aaaatacaaa aaattagccg
    11641 ggcgcggtgg cgggcgcctg tagtcccagc tactccggag gctgaggcag gagaatggcg
    11701 tgaacccggg aagcggagct tgcagtgagc cgagattgcg ccactgcagt ccgcagtccg
    11761 gcctgggcga cagagcgaga ctccgtctca aaataaataa ataaataaat aaataacatg
    11821 gataaaattt gttgtttgtt gttgttgttg ttgttgttgt tgttgttgag acggagtttc
    11881 gctcttgttg cccaggctgg agtgcaatgg cgcggtcttt gctcactgca acctctgcat
    11941 cccgggttca agctattctc ctgcctcagc ctcctgagta gctgggatta cacgatgcgc
    12001 caccaggcct gcctaacttt gtatttttgg tagaggcggg gtttctccat gttggtcagg
    12061 ctggtctcaa actcccgacc tcaggtgatc cgcccgcctc ggcctcccaa agtgctggga
    12121 ttacaggcat gagccactgc gcccagccaa atctgcattt tgataagtat gcattttact
    12181 atctaaggga gctaaaacat ttttattatt ttagcactgt aactttgctt ttcttctttg
    12241 ttttgttttt ttgagacaga gtctcactct gtcgcccagg ctggagtgca gtggcgtgat
    12301 ctcagctcac tgcaacctcc acctcctggg ttcaagcaat tctcctgcct cagcctcctg
    12361 agtagccagg attacaggtg cacgccacca cacccagcta atttatgtaa ttttatagag
    12421 ttgtggtttc accatattgg ccaagctggt ctagaactcc tggcctcaag tgatccacct
    12481 gcctcggcct cccaaagtgc ggggagacca gaattccacc cagaatgacc tctctgttcc
    12541 taggagtgtg acagcatttg ggggggaaaa tagcgtagag ttggaatttt taaaattcag
    12601 tttgacaaaa tgacttttag ctggagcatt aagtcccttt acatttaatg taataactga
    12661 tattacattt aagtataaat ttaaatctac cgtcttactt tgttttctat ttttgtcctt
    12721 tctatgttct cttttatgtc ctctctggat ttattacaga ttgtttgtgg cttttaaaaa
    12781 aagtcattgc ttgaatgatg agagcttata tttaaaaagg cagggggcta aaataaataa
    12841 ataatttaaa aaataaaaa
//
