LOCUS       NM_003917               2598 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens adaptor related protein complex 1 subunit gamma 2
            (AP1G2), transcript variant 1, mRNA.
ACCESSION   NM_003917
VERSION     NM_003917.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Tavares,L.A., da Silva,E.M., da Silva-Januario,M.E., Januario,Y.C.,
            de Cavalho,J.V., Czernisz,E.S., Mardones,G.A. and daSilva,L.L.
  TITLE     CD4 downregulation by the HIV-1 protein Nef reveals distinct roles
            for the gamma1 and gamma2 subunits of the AP-1 complex in protein
            trafficking
  JOURNAL   J Cell Sci 130 (2), 429-443 (2017)
   PUBMED   27909244
  REMARK    GeneRIF: Depletion of the gamma2 or mu1A (AP1M1) subunits of AP-1,
            but not of gamma1 (AP1G1), precludes Nef-mediated lysosomal
            degradation of CD4.
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Jurgens,M.C., Voros,J., Rautureau,G.J., Shepherd,D.A., Pye,V.E.,
            Muldoon,J., Johnson,C.M., Ashcroft,A.E., Freund,S.M. and
            Ferguson,N.
  TITLE     The hepatitis B virus preS1 domain hijacks host trafficking
            proteins by motif mimicry
  JOURNAL   Nat Chem Biol 9 (9), 540-547 (2013)
   PUBMED   23851574
REFERENCE   3  (bases 1 to 2598)
  AUTHORS   Aouizerat,B.E., Vittinghoff,E., Musone,S.L., Pawlikowska,L.,
            Kwok,P.Y., Olgin,J.E. and Tseng,Z.H.
  TITLE     GWAS for discovery and replication of genetic loci associated with
            sudden cardiac arrest in patients with coronary artery disease
  JOURNAL   BMC Cardiovasc Disord 11, 29 (2011)
   PUBMED   21658281
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2598)
  AUTHORS   Doring,T., Gotthardt,K., Stieler,J. and Prange,R.
  TITLE     gamma2-Adaptin is functioning in the late endosomal sorting pathway
            and interacts with ESCRT-I and -III subunits
  JOURNAL   Biochim Biophys Acta 1803 (11), 1252-1264 (2010)
   PUBMED   20708039
  REMARK    GeneRIF: Data show that gamma2-adaptin in MVB sorting specifically
            interacts with the ESCRT subunits Vps28 and CHMP2A.
REFERENCE   5  (bases 1 to 2598)
  AUTHORS   Rost,M., Doring,T. and Prange,R.
  TITLE     gamma2-Adaptin, a ubiquitin-interacting adaptor, is a substrate to
            coupled ubiquitination by the ubiquitin ligase Nedd4 and functions
            in the endosomal pathway
  JOURNAL   J Biol Chem 283 (46), 32119-32130 (2008)
   PUBMED   18772139
  REMARK    GeneRIF: gamma2-adaptin's ubiquitin-interacting motif mediates a
            specific physical interaction with the ubiquitin ligase Nedd4 and
            promotes ubiquitination of gamma2-adaptin
REFERENCE   6  (bases 1 to 2598)
  AUTHORS   Mattera,R., Ritter,B., Sidhu,S.S., McPherson,P.S. and
            Bonifacino,J.S.
  TITLE     Definition of the consensus motif recognized by gamma-adaptin ear
            domains
  JOURNAL   J Biol Chem 279 (9), 8018-8028 (2004)
   PUBMED   14665628
REFERENCE   7  (bases 1 to 2598)
  AUTHORS   Mattera,R., Arighi,C.N., Lodge,R., Zerial,M. and Bonifacino,J.S.
  TITLE     Divalent interaction of the GGAs with the Rabaptin-5-Rabex-5
            complex
  JOURNAL   EMBO J 22 (1), 78-88 (2003)
   PUBMED   12505986
REFERENCE   8  (bases 1 to 2598)
  AUTHORS   Page,L.J., Sowerby,P.J., Lui,W.W. and Robinson,M.S.
  TITLE     Gamma-synergin: an EH domain-containing protein that interacts with
            gamma-adaptin
  JOURNAL   J Cell Biol 146 (5), 993-1004 (1999)
   PUBMED   10477754
REFERENCE   9  (bases 1 to 2598)
  AUTHORS   Lewin,D.A., Sheff,D., Ooi,C.E., Whitney,J.A., Yamamoto,E.,
            Chicione,L.M., Webster,P., Bonifacino,J.S. and Mellman,I.
  TITLE     Cloning, expression, and localization of a novel gamma-adaptin-like
            molecule
  JOURNAL   FEBS Lett 435 (2-3), 263-268 (1998)
   PUBMED   9762922
REFERENCE   10 (bases 1 to 2598)
  AUTHORS   Takatsu,H., Sakurai,M., Shin,H.W., Murakami,K. and Nakayama,K.
  TITLE     Identification and characterization of novel clathrin
            adaptor-related proteins
  JOURNAL   J Biol Chem 273 (38), 24693-24700 (1998)
   PUBMED   9733768
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG701521.1, AB015318.1 and
            BQ073124.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_003917.4.
            
            Summary: Adaptins are important components of clathrin-coated
            vesicles transporting ligand-receptor complexes from the plasma
            membrane or from the trans-Golgi network to lysosomes. The adaptin
            family of proteins is composed of four classes of molecules named
            alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together
            with medium and small subunits, form a heterotetrameric complex
            called an adaptor, whose role is to promote the formation of
            clathrin-coated pits and vesicles. The protein encoded by this gene
            is a gamma-adaptin protein and it belongs to the adaptor complexes
            large subunits family. This protein along with the complex is
            thought to function at some trafficking step in the complex
            pathways between the trans-Golgi network and the cell surface.
            [provided by RefSeq, Aug 2017].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.1212482.1,
                                           SRR7410570.534190.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000397120.8/ ENSP00000380309.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                BG701521.1         49-62
            15-2535             AB015318.1         2-2522
            2536-2598           BQ073124.1         222-284
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q11.2"
     gene            1..2598
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /note="adaptor related protein complex 1 subunit gamma 2"
                     /db_xref="GeneID:8906"
                     /db_xref="HGNC:HGNC:556"
                     /db_xref="MIM:603534"
     exon            1..53
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            54..262
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..2416
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /note="isoform 1 is encoded by transcript variant 1;
                     gamma2-adaptin; clathrin-associated/assembly/adaptor
                     protein, large, gamma-2; AP-1 complex subunit gamma-like
                     2; adaptor related protein complex 1 gamma 2 subunit"
                     /codon_start=1
                     /product="AP-1 complex subunit gamma-like 2 isoform 1"
                     /protein_id="NP_003908.1"
                     /db_xref="CCDS:CCDS9602.1"
                     /db_xref="GeneID:8906"
                     /db_xref="HGNC:HGNC:556"
                     /db_xref="MIM:603534"
                     /translation="MVVPSLKLQDLIEEIRGAKTQAQEREVIQKECAHIRASFRDGDP
                     VHRHRQLAKLLYVHMLGYPAHFGQMECLKLIASSRFTDKRVGYLGAMLLLDERHDAHL
                     LITNSIKNDLSQGIQPVQGLALCTLSTMGSAEMCRDLAPEVEKLLLQPSPYVRKKAIL
                     TAVHMIRKVPELSSVFLPPCAQLLHERHHGILLGTITLITELCERSPAALRHFRKVVP
                     QLVHILRTLVTMGYSTEHSISGVSDPFLQVQILRLLRILGRNHEESSETMNDLLAQVA
                     TNTDTSRNAGNAVLFETVLTIMDIRSAAGLRVLAVNILGRFLLNSDRNIRYVALTSLL
                     RLVQSDHSAVQRHRPTVVECLRETDASLSRRALELSLALVNSSNVRAMMQELQAFLES
                     CPPDLRADCASGILLAAERFAPTKRWHIDTILHVLTTAGTHVRDDAVANLTQLIGGAQ
                     ELHAYSVRRLYNALAEDISQQPLVQVAAWCIGEYGDLLLAGNCEEIEPLQVDEEEVLA
                     LLEKVLQSHMSLPATRGYALTALMKLSTRLCGDNNRIRQVVSIYGSCLDVELQQRAVE
                     YDTLFRKYDHMRAAILEKMPLVERDGPQADEEAKESKEAAQLSEAAPVPTEPQASQLL
                     DLLDLLDGASGDVQHPPHLDPSPGGALVHLLDLPCVPPPPAPIPDLKVFEREGVQLNL
                     SFIRPPENPALLLITITATNFSEGDVTHFICQAAVPKSLQLQLQAPSGNTVPARGGLP
                     ITQLFRILNPNKAPLRLKLRLTYDHFHQSVQEIFEVNNLPVESWQ"
     exon            263..387
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            388..529
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            530..626
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            627..703
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            704..799
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            800..880
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            881..979
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1035
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1149
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1290
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1345
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1468
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1558
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            1559..1686
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1791
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1915
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..2051
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            2052..2215
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            2216..2314
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     exon            2315..2598
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2561..2566
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /note="hexamer: AATAAA"
     regulatory      2571..2576
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /note="hexamer: AATAAA"
     polyA_site      2582
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /note="major polyA site"
     polyA_site      2598
                     /gene="AP1G2"
                     /gene_synonym="G2AD"
                     /note="major polyA site"
ORIGIN      
        1 cccgtaccag gaagcgctgg ggggcagagg agcggagttg aggcagaagc cagtcaggat
       61 ggtggtgcct tcgctgaagc ttcaggacct catcgaagag attcgcgggg ccaagactca
      121 ggcccaggag cgggaggtga tccaaaagga gtgtgcccac atccgggcct ccttccgcga
      181 cggggaccca gtgcacaggc accggcagct ggccaaactg ctctacgtcc acatgttggg
      241 ctaccccgcc cactttggac agatggagtg cctgaaactg atcgcctcct ccagattcac
      301 agacaagagg gtgggctacc tgggggccat gcttctattg gatgagaggc acgatgccca
      361 cctgctcatt accaacagca tcaagaatga cctgagccag gggattcagc cagtacaagg
      421 cctggccttg tgcactttga gcaccatggg ctctgctgag atgtgccgag acctggcccc
      481 agaggtggag aaactgctcc tgcagcccag tccctacgtg cgcaagaagg ctattctgac
      541 tgcagtgcac atgatccgga aggtccctga actctccagt gtcttcctcc caccctgtgc
      601 ccaactgctt catgagcgtc accatggcat cctgctgggc accatcacgc tgatcacgga
      661 gctctgcgaa cgaagccctg cagccctcag gcacttccga aaggtggtac cccagctggt
      721 acacatcctc cggactctgg tgacaatggg atactccaca gaacacagca tatctggagt
      781 cagcgacccc ttcctgcagg tccagatact tcgtctgctt cggatcctgg gccggaacca
      841 cgaggagagc agtgagacca tgaatgactt gctggcccag gtggccacta acacggacac
      901 cagccgaaat gccggaaatg cggtcctgtt tgagacagta ctcaccatca tggatatccg
      961 ctctgcagct ggcctacggg ttctagctgt caacattctt ggtcgcttcc tactcaacag
     1021 tgacaggaac attaggtatg tagccctgac atcactgctt cgactggtgc agtctgatca
     1081 cagtgctgtg cagcggcatc ggcccactgt ggtggaatgt ctacgggaaa ctgatgcctc
     1141 cctcagccgg agagccctgg aactaagcct ggctctggta aatagctcca atgtgcgagc
     1201 catgatgcaa gagctgcagg cctttctgga gtcctgccct cctgacctac gggctgactg
     1261 tgcctcaggc atcctgctgg ctgcagagag gtttgctcca accaaacgct ggcacataga
     1321 caccatcctg catgtgctga caacggcggg cacccatgtg cgggatgatg cagtggccaa
     1381 cctgacccag ctgattgggg gggcccagga gctacatgcc tactctgtgc gccgcctcta
     1441 caatgccctg gcagaagaca tttcccagca accactggtg caggtggcag cctggtgcat
     1501 tggggagtat ggggacctcc tgctggcagg gaactgcgag gagattgagc cccttcaggt
     1561 ggacgaagag gaagtgctgg cattgctgga aaaggtgctg cagtcccaca tgtccctgcc
     1621 agccactcga ggatatgccc tcacagccct catgaagctc agcactcgcc tctgtgggga
     1681 caacaaccgc atccgccagg tggtgtccat ctacgggagc tgcttggacg tggagctgca
     1741 gcagcgggct gtggagtatg acacactctt ccggaaatac gaccacatga gggctgccat
     1801 cctggaaaaa atgcctcttg tggagcgaga tggccctcag gctgatgagg aagcaaagga
     1861 aagcaaagaa gcagcccagc tttcagaagc agccccagtg cccacagagc cccaggcctc
     1921 acagctcctg gatctgctag atctcctgga tggggcttct ggggatgtcc agcatcctcc
     1981 ccatctggac ccctccccag gaggtgccct ggtacacctg cttgaccttc cctgtgtacc
     2041 tccaccccca gctcccatcc cagatctcaa agtgtttgag cgtgagggag tacagctgaa
     2101 tctgtctttc attcgacccc ctgaaaaccc tgctttgctg ttaatcacca tcactgccac
     2161 caacttctca gagggtgatg tcacccattt catctgccag gctgctgtgc ccaagagtct
     2221 ccagctgcag ctgcaggccc ccagtgggaa cacagttcca gctcggggtg gccttcctat
     2281 cacccagctc ttcagaatcc tcaatcctaa caaggccccc ctgcggctaa agctgcgcct
     2341 cacctacgac cactttcacc agtcggtgca ggagatcttt gaggtgaaca acttgcctgt
     2401 ggaatcgtgg cagtaactgt ctccactcac agcctgaaat tctcctgtgt cccaaacccc
     2461 agggggcccc agcagcttcg aacctacacc tgagggctac cagcaggtgg cgctctggct
     2521 ttgcactgca aaaactgggg accagccccc ttctcccaca aataaagccc aataaagcct
     2581 gagaagtgag gaaagcca
//
